著者
佐野 元昭
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.23, no.2, pp.117-120, 2016 (Released:2016-08-31)
参考文献数
6

Early application of therapeutic hypothermia, rapid achievement of target cooling temperature were key factors for improving survival and neurological outcomes. In the present situation, targeted Temperature Management (TTM) is applied to only a minority of post-cardiac arrest patients. We have been looking forward to seeing widely applicable therapeutic approaches. We previously demonstrated that both inhalation of 2% hydrogen gas and TTM (33°C), starting at the beginning of cardiopulmonary resuscitation and given for 2 hours after return of spontaneous circulation (ROSC), yielded comparable improvement in survival and neurological deficit after ROSC in a rat model of cardiac arrest. However, in a clinical setting, hypothermia is applied after ROSC. We have confirmed that the benefit of hydrogen inhalation is similar when begun after ROSC.We would like to emphases a salutary effect of hydrogen gas inhalation on the neurological outcome after cardiopulmonary resuscitation independently of TTM in rats. Inhalation hydrogen gas therapy, either alone or in the combined with TTM, could represent a promising strategy to improve brain resuscitation for post cardiac arrest patients in the near future.
著者
木須 伊織
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.25, no.1, pp.35-40, 2018 (Released:2018-03-14)
参考文献数
13

Uterus transplantation (UTx) has become an alternative to gestational surrogacy and adoption for women with uterine factor infertility. Brännström et al. achieved the first human delivery after UTx in 2014 and to date a total of 8 babies have been born after UTx from living donors. This outcome attracted much attention worldwide and many countries have recently prepared for UTx. However, this procedure has many medical, ethical and social issues that require discussion prior to clinical application. Moreover, UTx is still in the experimental stage overseas and the safety and efficacy remain unclear despite several clinical applications. Despite the many issues to be resolved, this new organ transplantation technology will provide new hope for women with uterine factor infertility and further development of the technology is important for future reproductive and transplant medicine.
著者
本間 真人 平野 俊彦 湯沢 賢治 大河内 信弘 剣持 敬
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.24, no.1, pp.76-80, 2017 (Released:2017-03-31)
参考文献数
7

Questionnaire survey concerning practical use of generic immunosuppressive agents and the European Society for Organ Transplantation (ESOT) guideline was conducted in 130 clinical institutions including transplant hospitals where the members of The Japan Society for Organ Preservation and Biology were working. Forty five institutions answered the questionnaire survey (the recovery rate: 34.6%). Use of generic immunosuppressive agents in the institutions has been different among the agents; tacrolimus (6.7%), cyclosporin (8.9%) and mycophenorate mofetil (8.9%) and corticosteroid injection (51.1%). The results indicated that use of generic immunosuppressive agents, especially in the agents that require therapeutic drug monitoring (TDM) for dose adjustment, have not been spread in Japan. The most of the institutions (greater than 62%) agreed with the ESOT guideline for generic substitution of immunosuppressive agents. It is considered that the Japanese guideline is also required to prepare according to ESOT guideline, though the use of generic products have not been popular yet Japan.
著者
山田 陽城
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.25, no.1, pp.56-70, 2018 (Released:2018-03-14)
参考文献数
46

Nature is a very important resource for not only new drug discovery but also application of traditional medicines. Kampo (Japanese traditional) medicines have been used as the traditional formula in which multi-active ingredients may attack multiple target sites to recover complicated symptoms caused by disturbance of the body systems such as immune, neural and endocrine systems. Therefore, their multilateral actions are suitable to treat multifactorial diseases. Kampo medicines are using for the treatment depending on the patient’s clinical situation, either separately or to complement modern western medicine in Japan. Present review describes the concept and characterization of Kampo medicines, and introduces our pharmacological studies of Kampo medicines to clarify their action mechanism and active ingredients. The results may contribute for more evidence-based clinical applications of Kampo medicines.
著者
大見 和宏
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.20, no.1, pp.36-44, 2013-01-30 (Released:2014-11-26)
参考文献数
33

The blood brain barrier has important roles for physiological and pathological state in the brain. The molecules involved in barrier function have been identified. Now we can analyze these molecules by genomics and proteomics methods. The blood brain barrier is also called neurovascular unit which is composed of brain capillary endothelial cells, astrocytes, pericytes, neurons and microglia. They closely interacted with each other for the development and maintenance of blood brain barrier functions. In this review I will summarize some basic concepts and recent new findings about blood brain barrier:structure and function, neurological diseases, proteomics method, in vitro experimental system, and enzyme replacement therapy for lysosomal storage diseases/mucopolysaccharidosis.
著者
剣持 敬 伊藤 泰平 星長 清隆
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.20, no.2, pp.159-164, 2013-07-10 (Released:2014-11-26)
参考文献数
3

The organ procurement team must evaluate whether the organs such as liver, small intestine, pancreas, kidney can be procured or not by the donor conditions, past history, present illness, blood chemistry and ultrasonography. Before donor operation, multiorgan procurement teams should discuss about the order of organ procurement, crossclamp, blood drainage, perfusion technique and the division of the vessels. Donation of the abdominal organs from brain-dead donor is performed with the procedure of multiorgan donation technique. In the abdominal organs, small intestine is first extracted followed by the extraction of the liver and pancreas separately or en bloc. When the liver is solitary procured, en bloc procurement of pancreas and kidneys is popular in Japan. In the back table, the pancreas was isolated from the kidney grafts followed by the separation of both kidney grafts. Both kidneys are divided by the division of the aorta, the vena cava and the connective tissues. The kidney grafts are perfused with UW solution before packing on ice.
著者
半田 宏 伊藤 拓水 安藤 秀樹
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.21, no.2, pp.134-140, 2014-07-10 (Released:2014-11-10)
参考文献数
9

Half a century ago, the sedative thalidomide caused one of the worst notorious drug disasters in history, with more than 10,000 babies born with deformities. The drug is now used in the treatment of multiple myeloma. Recently new thalidomide derivatives called immunomodulatory drugs (IMiDs) have been developed. Among them, lenalidomide and pomalidomide have excellent anti-cancer activity. However, the use of them is limited due to its potent teratogenic activity. The mechanism by which IMiDs including thalidomide induce birth defects and therapeutic effects was a long-standing question. Using an affinity beads technology we originally developed, we have identified cereblon(CRBN)as a primary target of IMiDs. CRBN forms an E3 ubiquitin ligase complex. IMiDs alter the activity and induce various biological effects such as teratogenicity, anti-cancer and immunomodulation.Understanding IMiDs and CRBN may lead to uncover new therapeutic pathways for overcoming refractory cancer diseases.
著者
若杉 安希乃
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.23, no.1, pp.46-52, 2016 (Released:2016-06-22)
参考文献数
8

Of 627 physicians targeted by Japan Kampo Medicines Manufacturers Association (JKMA) as part of “Factual Investigation on Prescription of Kampo Medicines 2011,” 89% prescribed Kampo medicines on a daily basis. The department that ranked the highest in prescribing Kampo medicines was Obstetrics and Gynecology (97%), followed by Internal Medicine (96%), Surgery (95%), Neuropsychiatry (92%), and Orthopedics (90%). Thus, the majority of the departments ranked high (above 90%). However, the results of a questionnaire survey administered by Nikkei TRENDY to 1000 doctors asking, “Will you take Kampo medicines when you fall sick?” 31% of them take Kampo medicine positively, 39% - not basically, and17%- not at all. That is, majority of the doctors answered, “No, I do not take Kampo medicines.” The reasons were “poor evidence” and “ambiguous diagnoses.” Although Kampo is a traditional Japanese medicine, it is not completely appreciated owing to negative impressions such as “poor evidence”, “unscientific”, and so on. On the other hand, manufacturers often promote health foods or cosmetics, taking advantage of their positive impression such as “less side effects”, “mild effects”, and so on. In this review, the outline the details of Kampo medicine and highlight the accumulated evidence concerning Kampo in an attempt to clearing the misunderstanding about Kampo. It is required that doctors and pharmacists promote high-quality clinical practice and research by adopting a viewpoint on Kampo medicine to uphold this traditional medicine.
著者
竹内 朋代 野口 雅之 川上 康 大河内 信弘
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.23, no.1, pp.21-28, 2016 (Released:2016-06-22)
参考文献数
9

Biobank is the facility for preserving and providing biospecimens such as human cells, tissues and blood with their clinical data. Every year the establishment biobank has increased all over the world. The human biospecimens are rarely provided for researchers who want to use them for life-science research in Japan; the preservation is done well, though. Recently the ethical guidelines for life-science research using of human biospecimens have been amended. Through this revision, researchers can be easy to use these human biospecimens than before. It is expected this will provide the chances to progress life-science research. The next step of banking the human biospecimens is to examine the quality of the samples and to prepare the standard operating procedure. It is also extremely expected that biobank will be the basis for research toward the highly advanced medical technology.
著者
陳 豊史 本山 秀樹 土屋 恭子 高萩 亮宏 齊藤 正男 田中 里奈 宮本 英 大畑 恵資 高橋 守 近藤 健 青山 晃博 伊達 洋至
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.23, no.2, pp.164-167, 2016 (Released:2016-08-31)
参考文献数
15

To overcome the severe donor shortage in lung transplantation, it is crucial to use the marginal donor lungs and lungs from cardiac-death donors, which are supposed to be actually or potentially damaged. To utilize these damaged donor lungs safely, it is important to evaluate such donor lungs objectively before lung transplantation. Recently, ex vivo lung perfusion (EVLP) was developed and has been introduced internationally. Several clinical trials are also ongoing in Europe and North America. In this situation, we also established EVLP in small and large animals. Then, we have investigated the quality of donor lungs using EVLP and tried to treat the donor lungs during EVLP before lung transplantation. We also established several injured lung models and performed various experiments using EVLP, proving that damaged donor lungs could be treated with several drugs using EVLP. Furthermore, we investigated the detection of the regional lung damage using thermography. Herein, we demonstrated our findings and discussed the future of EVLP.
著者
小林 英司
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.19, no.1, pp.107-108, 2012-07-10 (Released:2014-11-26)
参考文献数
3
著者
田上 辰秋 尾関 哲也
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.24, no.1, pp.54-60, 2017 (Released:2017-03-31)
参考文献数
24

Drug delivery systems (DDS) which deal with nanoparticles or micro-particles have been extensively developing to meet the needs of patients. In this review, we introduced various kinds of drug carriers. Liposome is a phospholipid-based biocompatible nanocarrier which is one of well-studied for several decades. Liposomes can encapsulate the drugs with narrow therapeutics window to prevent the severe side effects. Polyethylene glycol (PEG)-loaded liposomes can prolong the blood circulation time and shows passive accumulation into tumor tissue (Passive targeting), which is called as enhanced permeability and retention effect. Specific ligand-conjugated liposomes have the ability to make the liposomes home the target cells and tissues (Active targeting). Specific stimuli-responsive liposomes can be used to control the drug release to target site by the stimulation as trigger (Triggered release). In addition, the characteristics of other nanoparticles including albumin-based nanoparticles, micelles, dendrimer, emulsions, metal nanoparticles and polymer particles were introduced. Current progress of DDS technology would result in the emergence of new nano- and micro carriers with different platforms. The information about functional nanoparticles and microparticles will be useful for the medical staffs to understand the current and future particle-based medicine.
著者
深井 原 島田 慎吾 若山 顕治 嶋村 剛 山下 健一郎 藤堂 省 武冨 紹信
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology (ISSN:13405152)
巻号頁・発行日
vol.20, no.2, pp.176-180, 2013-07-10 (Released:2014-11-26)
参考文献数
17
被引用文献数
1

Extended criteria donor(ECD)グラフトの安全な利用のために,新しい臓器修復,保存法の実用化が求められている.単純冷保存に対する臓器灌流の優位性は腎移植,肝移植で示された.心停止グラフトは,摘出後ただちに酸素化灌流すれば灌流温度によらず修復されるが,灌流前に冷保存が加わると修復されがたい.本稿では,腎臓,肝臓の単純冷保存と臓器灌流の知見を整理し,実用性の高い方法論を確立するために克服しなければならない課題を明らかにすることを目的とする.
著者
圷 尚武 丸山 通広 大月 和宣 石田 健倫 齋藤 友永 西郷 健一 長谷川 正行 青山 博道 剣持 敬 野口 洋文
出版者
一般社団法人 日本臓器保存生物医学会
雑誌
Organ Biology
巻号頁・発行日
vol.24, no.2, pp.175-179, 2017

1型糖尿病に対する膵島移植が国内で始まったものの,依然として大きな問題はドナー不足である.その解決手段のひとつとして,心停止下ドナーのような,条件の悪いいわゆるマージナルドナーより採取された膵臓の有効な利用である.LifePort<sup>TM</sup>(LP)はOrgan Recovery Systems社により開発された臓器搬送用の持続冷却灌流装置であり,現在欧米において障害を伴った腎臓の搬送に臨床応用され,その有効性が確認されている.本装置はマージナルドナーから採取された臓器移植,特に虚血再潅流障害を受けやすい膵臓の保存に有用で,膵島移植の安全性や成績の向上に有効であると考えられる.本研究では,ビーグル犬を使用し,障害膵モデルより膵臓を摘出し,LPにより持続冷却灌流保存することにより,膵臓の障害が最小限に抑えられ,従来法であるUW液や2層法による浸漬保存に比べて分離膵島の収量や機能が改善でき,臨床膵島移植に応用し,成績向上に寄与することを目的とした.[方法]ビーグル犬から30分の温阻血時間をおいて全膵を摘出し,LPにて24時間持続冷却灌流保存(LP群),UW液にて24時間冷却浸漬保存(UW群),2層法にて24時間冷却浸漬保存(2層法群)し,膵島分離を行い,その形態・機能を比較検討した.[結果]形態的には,LP群でより大きく,形の良い膵島が分離できた.また,膵島収量については,純化前後でLP群においてUW群や2層法群に比べて良好であった.また,分離膵島のインスリン分泌能を評価するStatic Incubationの結果でも,LP群でUW群や2層法群に比べて良好な結果であった.[結論]心停止下ドナーよりの膵島移植を想定したモデルにおいて,LPによる障害膵の持続冷却灌流保存は,UW液や2層法による冷却浸漬保存に比べて,分離膵島の収量や機能保持に優れていることが示唆された.しかし,臨床応用のためには,さらなる保存条件や膵島分離法の改善が必要であると考えられた.