著者

庫本 高志 横江 繭子 矢ヶ崎 佳代子 KAWAGUCHI Tatsuya KUMAFUJI Kenta SERIKAWA Tadao

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.59, no.2, pp.147-155, 2010

被引用文献数

1 23

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To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (<i>am</i>), Black eye (<i>Be</i>), grey (<i>g</i>), Pearl (<i>Pel</i>), siamese (<i>sia</i>); 1 coat pattern, head spot (<i>hs</i>); 2 coat textures, Rex (<i>Re</i>), satin (<i>sat</i>); and an ear pinnae malformation, dumbo (<i>dmbo</i>). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): <i>am</i> to Chr 1, <i>sia</i> to Chr 1, <i>sat</i> to Chr 3, <i>Re</i> to Chr 7, <i>g</i> to Chr 8, <i>dmbo</i> to Chr 14, and <i>hs</i> to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for <i>sia</i>. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.<br>

著者

Tamio OHNO Yuki MIYASAKA Masako KUGA Kaori USHIDA Miyoko MATSUSHIMA Tsutomu KAWABE Yoshiaki KIKKAWA Masashi MIZUNO Masahide TAKAHASHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0185, (Released:2019-03-15)

被引用文献数

3

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Malaria is caused by Plasmodium parasites and is one of the most life-threatening infectious diseases in humans. Infection can result in severe complications such as cerebral malaria, acute lung injury/acute respiratory distress syndrome, and acute renal injury. These complications are mainly caused by P. falciparum infection and are major causes of death associated with malaria. There are a few species of rodent-infective malaria parasites, and mice infected with such parasites are now widely used for screening candidate drugs and vaccines and for studying host immune responses and pathogenesis associated with disease-related complications. We found that mice of the NC/Jic strain infected with rodent malarial parasites exhibit distinctive disease-related complications such as cerebral malaria and nephrotic syndrome, in addition to a rapid increase in parasitemia. Here, we focus on the analysis of host genetic factors that affect malarial pathogenesis and describe the characteristic features, utility, and future prospects for exploitation of the NC/Jic strain as a novel mouse model for malaria research.

著者

Jiyuan SI Ranran MENG Peng GAO Feifei HUI Yu LI Xianhu LIU Bin YANG

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0089, (Released:2018-10-23)

被引用文献数

12

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Percutaneous coronary intervention (PCI) is main treatment for acute coronary syndrome (ACS). However, restenosis caused by PCI-induced injury influences the outcome of patients. Linagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, has been reported to ameliorate intimal hyperplasia post vascular injury. The underlying mechanisms by which linagliptin protects against balloon injury are unclear and require to be explored. Herein, Wistar rats with carotid artery balloon injury were given 1, 2 or 3 mg/kg/day linagliprin for 6 weeks. We found that linagliptin attenuated vascular injury-mediated neointima formation in rats without affecting body weight and blood glucose levels. ELISA results indicated that linagliptin significantly reduced overproduction of cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 post balloon injury. By detecting the level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), we found that linagliptin prevented balloon injury-induced oxidative stress. Additionally, linagliptin decreased the level of Kelch ECH-associating protein 1 (KEAP1) compared with injury group. Results of Western blots and electrophoretic mobility shift assay (EMSA) demonstrated that linagliptin augmented nuclear accumulation of nuclear factor-E2-related factor 2 (NRF2) and its binding ability to target genes in rats with balloon injury. Moreover, heme oxygenase-1 (HO-1) and NAD(P)H quinine oxidoreductase 1 (NQO1), two downstream targets of NRF2, were further up-regulated after linagliptin treatment compared with injury group. In conclusion, our data suggest that linagliptin protects carotid artery from balloon injury-induced neointima formation and activates the NRF2 antioxidant pathway.

著者

Hao ZHANG Zhong-Li LI Xiang-Zheng SU Li DING Ji LI Heng ZHU

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.17-0137, (Released:2018-03-08)

被引用文献数

4

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Rabbit mesenchymal stem cells (MSCs) are important seed cells in regenerative medicine research, particularly in translational research. In the current study, we showed that rabbit subchondral bone is a reliable source of MSCs. First, we harvested subchondral bone (SCB) from the rabbit knee-joint and initiated the MSC culture by cultivating enzyme-treated SCB. Adherent fibroblast-like cells that outgrew from SCB fulfill the common immuno-phenotypic criteria for defining MSCs, but with low contamination of CD45+ hematopoietic cells. Interestingly, differentiated SCB-MSCs expressed osteogenic and chondrogenic markers at significantly higher levels than those in bone marrow cell suspension-derived MSCs (BMS-MSCs) (P<0.05). No differences in the expression of adipogenic markers between SCB-MSC and BMS-MSC (P>0.05) were observed. Moreover, the results of the colony forming unit-fibroblast assay and sphere formation assay demonstrated that the SCB-MSCs had increased self-renewal potential. SCB-MSCs expressed higher levels of the stemness markers Nanog, OCT4, and Sox-2 compared to in BMS-MSCs (P<0.05). Furthermore, the results of both the CCK-8-based assay and CFSE dilution assay showed that SCB-MSCs exhibited enhanced proliferative capacity. In addition, SCB-MSCs exhibited higher phosphorylation of extracellular signal-related kinase/mitogen-activated protein kinase signaling, which is closely related to MSC proliferation. In conclusion, we identified SCB-MSCs as a novel stem cell population that met the requirements of MSCs; the unique properties of SCB-MSC are important for the potential treatment of tissue damage resulting from disease and trauma.

著者

都築 政起 若杉 昇

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.37, no.2, pp.137-144, 1988-04-01 (Released:2010-08-25)

参考文献数

9

被引用文献数

3 3

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ニホンウズラの新しい突然変異「back-drawer」は, 腹部を覗き込むようにして首を腹側に強く曲げ, しゃがみ込んだ姿勢で, 常時後ずさりをする行動を示し, 時折前方に回転する。この異常は, 孵化時から8週齢の間に発症し, 症状は一時的な発作として出現するのではなく, 一定期間持続した。発症個体は3つのタイプに分けられ, 1つは, 孵化時既に症状を示しており5日齢以前に死亡するもので, 他の2つは2週齢時以降に発症し, 発症後早期に死亡するもの, および長期間生存するものであった。後者では, 症状が漸次軽減し, やがて正常個体との区別が困難となるものもあった。雄は, 外見的に性成熟に達した後も, 異常行動を示す期間は繁殖力をもたないが, 回復後は繁殖力をもつようになる。一方, 雌はほとんど産卵せず早期に死亡する傾向がある。後ずさり形質は, 常染色体性の2対の劣性遺伝子によって支配されていると考えられる。

著者

田嶋 嘉雄

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.33, no.1, pp.1-23, 1984-01-01 (Released:2010-08-25)

被引用文献数

1 1

著者

Tomoyuki SATO Tomoyo OCHIISHI Sayaka HIGO-YAMAMOTO Katsutaka OISHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0104, (Released:2023-12-14)

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Disturbances in sleep-wake and circadian rhythms may reportedly precede the onset of cognitive symptoms in the early stages of Alzheimer’s disease (AD); however, the underlying mechanisms of these AD-induced sleep disturbances remain unelucidated. To specifically evaluate the involvement of amyloid β (Aβ) oligomers in AD-induced sleep disturbances, we examined circadian and sleep phenotypes using an Aβ-GFP transgenic (Aβ-GFP Tg) mouse characterized by intracellular accumulation of Aβ oligomers. The circadian rhythm and free-running period of wheel running activity were identical between Aβ-GFP Tg and littermate wild-type mice. The durations of rapid eye movement (REM) sleep were elongated in Aβ-GFP Tg mice; however, the durations of non-REM sleep and wakefulness were unaffected. The Aβ-GFP Tg mice exhibited shifts in the electroencephalogram (EEG) power spectra toward higher frequencies in the inactive light phase. These findings suggest that the intracellular accumulation of Aβ oligomers might be associated with sleep quality; however, its impact on circadian systems is limited.

著者

Mohammad Abdul AWAL Mohammad ASADUZZAMAN Mohammad Khairul ANAM Mohammad Abdul Aziz PRODHAN Masamichi KUROHMARU

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.50, no.4, pp.349-352, 2001 (Released:2003-11-06)

参考文献数

18

被引用文献数

1 1

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Arterial supply to the stomach of dogs indigenous to Bangladesh was investigated by using latex. The hepatic, left gastric and splenic arteries sent their major branches to the stomach. The cranial and caudal branches of the left gastric artery supplied the lesser curvature of the stomach. The right gastric, and right and left gastroepiploic arteries also sent their branches to both the lesser and greater curvatures. Six or seven short gastric arteries from the splenic artery supplied the greater curvature. Anastomoses between the left and right gastric, between the left and right gastroepiploic, and between short gastric arteries and left gastric arteries were observed.

著者

矢沢 肇 梅沢 英彦 倉益 茂実 宮嶋 正康

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.35, no.2, pp.203-206, 1986-04-01 (Released:2010-08-25)

参考文献数

6

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日本生物科学研究所付属実験動物研究所において全兄妹交配により, JWY-NIBS及びNWY-NIBSの2近交系が確立された。それらの起源, 確立過程, 成熟時月齢, 成熟時体重及び標識遺伝子は下記の如く要約される。1.JWY-NIBS: 起源: 東京都の高尾山付近で繁殖され, その後府中市の農家で維持されていたウサギを起源とする。近交開始年月: 1964年4月。近交系確立年月: 1981年6月。成熟時月齢: メス7カ月, オス7.5カ月。成熟時体重: メス2.8~3.0kg, オス2.7~2.9kg。生化学的標識遺伝子: ヘモペキシンHxs型, エステラーゼEst-1s型, α-プロテインはF型にそれぞれ固定していた。2.NWY-NIBS: 起源: 米国ジャクソン研究所より1967年に導入された系統III, ニュージーランドホワイト種を超源とする。近交開始年月: 1967年11月。近交系確立年月: 1982年7月。成熟時月齢: メス7.5カ月, オス8カ月。成熟時体重: メス2.8~3.0kg, オス2.9~3.1kg。生化学的標識遺伝子: ヘモペキシンHxF型。エステラーゼEst-1s型及びEst-2f型, α-プロテインはS型にそれぞれ固定していた。

著者

Yutaka MASUDA Minoru SUZUKI Yusuke AKAGAWA Takaubu TAKEMURA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.48, no.3, pp.209-211, 1999 (Released:2003-11-22)

参考文献数

4

被引用文献数

9 9

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We demonstrated emotional piloerection in mice given conditioned fear stress by means of a pass-through apparatus. The emotional piloerection was first assessed in mice of different ages. The results showed that the piloerection changed with age. Pharmacological studies showed that the piloerection was inhibited by an alpha 1-adrenoceptor antagonist prazosin, but, surprisingly, was not inhibited by anxiolytic diazepam. These findings strongly suggest that the neuronal system of piloerection is different from that of freezing behavior, and that the neuronal system of piloerection develops with age.

著者

Michiko HIGASHI Saori TAHARABARU Yushi U. ADACHI Maiko SATOMOTO Takahiro TAMURA Naoyuki MATSUDA Aiji SATO-BOKU Masahiro OKUDA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0010, (Released:2023-06-02)

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Administration in a lipid emulsion can modify the pharmacodynamics of drugs via a process known as lipid resuscitation. However, the detailed mechanism remains unclear. We studied the volume and another pharmacodynamic effect, the lipid sink, using propofol and thiamylal. Male adult mice (ddY) were intravenously administered 10 mL/kg propofol or thiamylal diluted with physiological saline, 10% soybean oil, or 20% soybean oil. The 50% effective dose (ED50) for achieving hypnosis was calculated using probit analysis. To investigate the volume effect, 0, 10, or 20 mL/kg of saline or soybean oil was administered, either simultaneously or beforehand. Next, a two- or three-fold dose of the anesthetics was administered and the durations of anesthesia were measured. Finally, at 30 s after the first injection, supplemental soybean oil was administered. The mean (± standard error) ED50 values of propofol and thiamylal were 5.79 mg/kg (0.61) and 8.83 mg/kg (0.84), respectively. Lipid dilution increased the ED50 values of both anesthetics. After injection of a dose two-fold the ED50 value, the respective mean (± standard deviation) durations of anesthesia were 125 ± 35 s and 102 ± 38 s. Supplemental administration of soybean oil significantly shortened the duration of anesthesia of propofol, but not that of thiamylal. The results indicate that administration of a lipid emulsion vitiated the anesthetic effect of propofol by reducing the non-emulsified free fraction in the aqueous phase, which may elucidate the lipid resuscitation likely caused by the lipid sink mechanism.

著者

Natalia GOGOLEVA Zeynab Javanfekr SHAHRI Atsushi NODA Ching-Wei LIAO Arata WAKIMOTO Yuri INOUE Hyojung JEON Satoru TAKAHASHI Michito HAMADA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0044, (Released:2023-05-12)

被引用文献数

1

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Adeno-associated virus serotype 9 (AAV9) has become a popular tool for gene transfer because of its ability to cross the blood-brain barrier and efficiently transduce genetic material into a variety of cell types. The study utilized GRR (Green-to-Red Reporter) mouse embryos, in which the expression of iCre results in the disappearance of Green Fluorescent Protein (GFP) expression and the detection of Discosoma sp. Red Fluorescent Protein (DsRed) expression by intraplacental injection. Our results demonstrate that AAV9-CMV-iCre can transduce multiple organs in embryos at developmental stages E9.5–E11.5, including the liver, heart, brain, thymus, and intestine. These findings suggest that intraplacental injection of AAV9-CMV-iCre is a viable method for the widespread transduction of GRR mouse embryos.

著者

松崎 哲也 安田 幸雄 野中 衆一

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.38, no.4, pp.337-341, 1989-10-01 (Released:2010-08-25)

参考文献数

6

被引用文献数

5 8

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スナネズミの毛色に関する遺伝学的研究を行った。まず, 白色について遺伝分析を行うために, 白色と野生色とを交配しF1, F2およびバッククロス個体を得た。その結果, F1はすべて野生色, F2では野生色と白色が3: 1に, またバッククロスでは1: 1に分離した。次に, 本研究で発見された黒色 (非野生色) の個体をもちいて野生色の遺伝分析を行った。野生色と黒色との交配でF1およびF2を得た。F1はすべて野生色を, またF2では野生色と黒色が3: 1に分離した。なお, 交配実験で得られた各世代における毛色の性比を調べたが偏りはなかった。以上の結果は, 白色は常染色体性の劣性遺伝子支配を, また野生色は非野生色に対して優性で常染色体性の遺伝子支配を受けることを示した。また, 黒色個体の出現によって, brown遺伝子座の存在が示唆された。以上を総合すると, スナネズミの毛色はマウス・ラットと同様に3種類の基本遺伝子座, a (non-agouti/agouti) , b (brown/black) およびc (albino/colored) で説明できることを強く示唆した。

著者

Koji KAWAKAMI Kenji KOJIMA Ikuyo MAKINO Ikuo KATO Masaharu ONOUE

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.56, no.4, pp.301-307, 2007 (Released:2007-07-27)

参考文献数

23

被引用文献数

7 12

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p-Cresol is a metabolite of aromatic amino acid metabolism produced by intestinal microflora, and its formation is influenced by intestinal conditions. Fasting drastically changes intestinal conditions. However, the effect of fasting on p-cresol production is unclear. In this study, serum and cecal p-cresol levels were determined in non-fasted rats and in rats fasting for either 12 or 18 h. Serum p-cresol increased significantly with 12-h fasting (3.44 ± 2.15 nmol/ml; P<0.05) and 18-h fasting (5.40 ± 2.20; P<0.001) as compared to the level in the non-fasted rats (1.02 ± 0.50). Cecal p-cresol levels of the 12-h fasted (272.6 ± 313.2 nmol/cecum) and 18-h fasted rats (436.6 ± 190.8; P<0.01) were higher than those in non-fasted rats (27.1 ± 21.9). The total cecal protein in content did not change with 18-h fasting. However, the cecal protein concentration increased significantly with fasting (P<0.001), and correlated closely with total cecal p-cresol contents (P<0.001). These results indicate that fasting enhances p-cresol production in the rat cecum, resulting in accumulation of serum p-cresol. We presume that the increase in p-cresol produced by fasting is related to the enhancement of bacterial nitrogen metabolism via an increased concentration of endogenous protein in the cecum.

著者

Masahiro TAGAWA Yasushi HARA Hiroyasu EJIMA Shinobu KOMORI Toru R. SAITO Kazuaki W. TAKAHASHI Kyoji HIOKI Susumu EBUKURO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.44, no.1, pp.49-55, 1995 (Released:2003-12-23)

参考文献数

19

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This study was undertaken to evaluate the sedative effect of medetomidine, an α2-adrenoceptor agonist, and the counteractive effect of atipamezole, an antagonist to medetomidine, in house musk shrews (Suncus murinus). Two hundred, 300, 400, or 600 μg/kg of medetomidine was intraperitoneal injected into 89 house musk shrews. A sedative effect was produced in one to two minutes after injection. The dose-dependent prolongation of the sedative duration and the dose-dependent appearance of a hypothermic effect were demonstrated. With 200 μg/kg of medetomidine, the sedative effect obtained was not adequate in some of the animals. With 300 μg/kg and above, a stable sedative state was induced in all the animals. The duration of sedation in the house musk shrews was much longer (p<0.01) in males than in females. This suggested the higher susceptibility of male house musk shrews to this drug. The sedative effect and hypothermia obtained with 400 μg/kg of medetomidine were completely counteracted by more than 2.0 mg/kg of atipamezole. With 0.5 and 1.0 mg/kg of atipamezole, only a partial antagonistic action was produced. Transient vomiting appeared in 4.5% of the house musk shrews at approximately one minute after injection of medetomidine. This side-effect had occurred before the sedative effect was obtained, and was not serious enough to be a problem. None of the 89 house musk shrews died in this experiment. The above results show that the combination of medetomidine and atipamezole is a highly effective and safe anesthetic treatment which permits easy handling of house musk shrews.

著者

Sarah Annie GUÉNETTE Marie-Chantal GIROUX Pascal VACHON

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.62, no.2, pp.87-92, 2013 (Released:2013-04-25)

参考文献数

52

被引用文献数

20 27

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Frogs possess pain receptors and pathways that support processing and perception of noxious stimuli however the level of organization is less well structured compared to mammals. It was long believed that the experience of pain was limited to ‘higher’ phylums of the animal kingdom. However, it is now commonly accepted that amphibians possess neuro-anatomical pathways conductive of a complete nociceptive experience. Xenopus laevis frogs have been one of the most popular aquatic research models for developmental studies and genetic research. These frogs have been extensively use in research for their eggs, that can be collected following hormonal stimulation either naturally or by surgical intervention. Many anaesthetics have been used in amphibians such as bath solutions of MS-222, benzocaine and eugenol as well as systemic injections of ketamine or tiletamine, barbiturates, propofol and gas administrations of methoxyflurane, halothane and isoflurane. Most of these anaesthetic drugs produce variability in depth and duration of anaesthesia. MS-222 appears to be one of the most reliable anaesthetics. This review will focus on the evidence of pain perception in frogs and will compare the effectiveness and limitations of different anaesthetics used in Xenopus leavis frogs.

著者

海老野 耕一 首藤 康文 高橋 和明

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.42, no.4, pp.611-613, 1993-10-01 (Released:2010-08-25)

参考文献数

12

被引用文献数

4 7

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ウサギは食糞行動を示す代表的動物種である。従来よりウサギは軟糞・硬糞の2種類の糞を排泄し, その内軟糞だけを摂取すると報告されてきた。しかし, ウサギの食糞行動を詳細に観察したところ, ウサギは軟糞同様に硬糞も摂取していた。従来, ウサギはその栄養価値が高いゆえ軟糞のみを摂取すると言われていたが, 栄養価値の低い硬糞も食べていることが本実験で明らかになった。得られた成績から, ウサギの食糞行動は結腸もしくは直腸内にあってそれらの壁を内側より伸展する糞そのものの刺激によって誘起される可能性があると考えられた。

著者

伊藤 豊志雄 岩井 浤

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.30, no.4, pp.491-495, 1981-10-20 (Released:2010-08-25)

参考文献数

12

被引用文献数

1 1

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センダイウイルスに感染したICRマウスの体重および体温は減少し, その減少の程度は投与ウイルス量に比例した。重症例においては, ヘモグロビン, ヘマトクリット, 赤血球数, 白血球数とロイシンアミノペプチダーゼの上昇と, 網赤血球と乳酸脱水素酵素の減少をみた。

著者

Yating LUO Ruyue CHENG Huijing LIANG Zhonghua MIAO Jiani WANG Qingqing ZHOU Jianguo CHEN Fang HE Xi SHEN

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.21-0182, (Released:2022-07-06)

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In this study, C57BL/6J male mice were fed normal chow (NC; control) or a high-fat diet (HFD) for 12 weeks, and HFD mice were supplemented with oral administration of Streptococcus thermophilus MN-ZLW-002 (HFD + MN002; n=20/group. Body weight, visceral fat, blood glucose, blood lipids and liver lipid deposition increased in the HFD group, and the composition of gut microbiota, cecum short-chain fatty acids and fecal bile acids (BAs) also changed. Oral-fed MN-002 increased the relative abundances of Ruminococcaceae, Lachnospiraceae and Streptococcaceae and improved blood glucose, liver cholesterol deposition, and serum IL-10, CCL-3 and the fecal BAs composition. In conclusion, the high-fat diet changed the composition of bile acids by shaping the gut microbiota into an obese type, leading to metabolic disturbances. Streptococcus thermophilus MN-ZLW-002 regulated gut microbiota by adjusting the composition of bile acids and improved the perturbation caused by high-fat diets. However, the effect of MN002 observed in animal experiments needs to be verified by long-term clinical trials.

著者

小野 孝浩 鈴木 通弘 成田 勇人 長 文昭

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.38, no.4, pp.293-296, 1989-10-01 (Released:2010-08-25)

参考文献数

14

被引用文献数

1 1

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カニクイザル新生仔の健康管理上の一指標として体温 (直腸温) を取り上げ, 新生仔における体温の変動についていくつかの基礎的検討を試みた。生後0日齢の新生仔ザルの体温は, 母ザルにより上手に哺育されていたもの183頭では33.0~37.7℃, 哺育されていなかったもの21頭では24.1~34.8℃の範囲にあった。母ザルの哺育能が良好である場合の新生仔の娩出直後からの体温変化をみると, 娩出時は約36℃と母ザルの体温に近似していたが, その後急激に下降し40~50分後に32~33℃で最低となった。その後, 新生仔の体温は上昇に転じ, 生後180~240分で36~37℃となり安定した。一方, 帝王切開術にて娩出し保温せずに個別ケージに収容された新生仔の体温は, 娩出直後37~38℃であったものが, 120分後には29~32℃にまで低下した。