著者

大塚 潤一 豊満 義邦 西中川 駿

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.29, no.4, pp.441-455, 1980

被引用文献数

1

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キュウシュウノウサギ (以下<I>Lepus</I>) , アマミノクロウサギ (<I>Pentalagus</I>) および家兎JW-NIBSの頭蓋骨・胴骨を形態学的ならびに骨計測学的に検索した。<BR>1) 尾椎数は<I>Lepus</I>が10~12個, <I>Pentalagus</I>が10~11個およびJW-NIBSが15~17個である。<BR>2) <I>Pentalagus</I>の上顎臼歯数が5個という従来の記載は, この種属の識別用形質とは認められない。<BR>3) <I>Pentalagus</I>の眼窩および鼓室胞は極端に小さい。<BR>4) JW-NIBSの脳頭蓋の長さおよび頸椎, 胸椎, 腰椎の椎孔の幅は, 他の二者より小さい傾向を示す。<BR>5) 腰椎の棘突起および横突起の形状および大きさは, 三者間に明白な差異がある。<BR>6) 骨の計測値の変異係数は, JW-NIBSが最も小さい。

著者

Yui SHIMOMI Yasuhiko KONDO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.19-0161, (Released:2020-06-30)

被引用文献数

3

---

Among the intact male rats, a subpopulation has been found to show little or no sexual behavior, even after experiencing several mating sessions. This study investigated whether sexually sluggish (SS) males show behavioral differences from normal copulatory (NC) males, other than those concerning sexual behavior. The olfactory preference of males was measured through the time spent displaying nose-poking behavior directed at sexually active males and estrous females for odor exploration in a three-chamber apparatus. Both the NC and SS males showed a significant preference for the odor of estrous females compared with that of male odors. However, SS males spent significantly less time nose-poking estrous females than NC males. The food-finding test was performed after overnight fasting. Our findings showed that all the NC males found the buried pellet within 5 min, whereas over 60% of the SS males failed to find it. The males were also tested for their ability to find a buried bag containing soiled bedding from estrous female cages. The bag was found by 80% of NC males, but only by 20% of SS males. Our results suggest that SS and NC male rats differ not only in sexual behavior but also in other functions such as olfaction.

著者

武下 政一 芝谷 光治 子林 孝司 新比恵 啓志 周参見 正行 有行 史男

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.42, no.3, pp.405-410, 1993

被引用文献数

1

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50kGy電子線照射滅菌飼料をラットに給餌し, 親ラットの生殖機能ならびに第一世代および第二世代の発生・発育に及ぼす影響を検討した.その結果, 電子線照射滅菌飼料群に特異的な変化は認められず, 今回用いた50kGy照射条件下では, 電子線滅菌飼料は既存のγ線照射滅菌飼料と同様, ラットの繁殖・育成用飼料として使用可能と判断された。

著者

青木 忍 天尾 弘実 斎藤 徹 斎藤 学 高橋 和明 多川 政弘

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.40, no.4, pp.569-572, 1991-10-01 (Released:2010-08-25)

参考文献数

6

---

モルモットの精巣摘出手術を腹部正中線および陰嚢の2ルートから行って, 手術時間, 術後の経過, 術創の治癒状況を比較し, いずれの手術ルートがモルモットに負担をかけずに, かつ安全に実施できるかを検討した。その結果, 手術時間には, 両者間に有意差は認められなかった。腹部正中線切開法では, 術後の経過が良く, 1週後の術創の治癒状況も良好であった。しかし, 陰嚢切開法では, 鞘膜臓側板や精巣白膜が薄く手術時に精巣実質を傷つけて精巣内容物を周囲に飛散させる危険性が高かった。そして, 治癒状況も不良であり, 5例中4例に陰嚢内化膿が見られた。以上の結果より, モルモットの精巣摘出手術法としては, 腹部正中線切開法が陰嚢切開法より適していた。

著者

坪井 基枝 林 良敬 城ヶ原 貴通 小倉 剛 村田 善晴 織田 銑一

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental animals (ISSN:13411357)

巻号頁・発行日

vol.58, no.1, pp.31-40, 2009-01-01

著者

織田 銑一 近藤 恭司

出版者

Japanese Association for Laboratory Animal Science

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.26, no.3, pp.273-280, 1977

被引用文献数

1

著者

Suvd BYAMBAA Hideki UOSAKI Hiromasa HARA Yasumitsu NAGAO Tomoyuki ABE Hiroaki SHIBATA Osamu NUREKI Tsukasa OHMORI Yutaka HANAZONO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.19-0120, (Released:2019-12-04)

被引用文献数

4

---

X-linked severe combined immunodeficiency (X-SCID) is an inherited genetic disorder. A majority of X-SCID subjects carries point mutations in the Interleukin-2 receptor gamma chain (IL2RG) gene. In contrast, Il2rg-knockout mice recapitulating X-SCID phenotype lack a large part of Il2rg instead of point mutations. In this study, we generated novel X-SCID mouse strains with small insertion and deletion (InDel) mutations in Il2rg by using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9. To this end, we injected Streptococcus pyogenes Cas9 (SpCas9) mRNA and single guide RNA targeting the exon 2, 3 or 4 of Il2rg into mouse zygotes. In the F0 generation, we obtained 35 pups and 25 out of them were positive for Surveyor assay, and most of mutants displayed dramatic reductions of T and B lymphocytes in the peripheral blood. By amplicon sequencing, 15 out of 31 founder mice were determined as monoallelic mutants with possible minor mosaicisms while 10 mice were mosaic. Finally, we established new strains with 7-nucleotide deletion and 1-nucleotide insertions in the exon 2 and the exons 3 and 4, respectively. Although no IL2RG protein was detected on T cells of exons 3 and 4 mutants, IL2RG protein was unexpectedly detected in the exon 2 mutants. These data indicated that CRISPR/Cas9 targeting Il2rg causes InDel mutations effectively and generates genetically X-SCID mice. Genetic mutations, however, did not necessarily grant phenotypical alteration, which requires an intensive analysis after establishing a strain to confirm their phenotypes.

著者

Yu-Hsin CHANG Megumi C. KATOH Ahmed M. ABDELLATIF Guli XIAFUKAITI Abdelaziz ELZEFTAWY Masami OJIMA Seiya MIZUNO Akihiro KUNO Satoru TAKAHASHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.19-0105, (Released:2019-12-02)

被引用文献数

1

---

Cre/loxP is a site-specific recombination system extensively used to enable the conditional deletion or activation of target genes in a spatial- and/or temporal-specific manner. A number of pancreatic-specific Cre driver mouse lines have been broadly established for studying the development, function and pathology of pancreatic cells. However, only a few models are currently available for glucagon-producing α-cells. Disagreement exists over the role of the MAFB transcription factor in glucagon expression during postnatal life, which might be due to the lack of α-cell-specific Cre driver mice. In the present study, we established a novel Gcg-Cre knock-in mouse line with the Cre transgene expressed under the control of the preproglucagon (Gcg) promoter without disrupting the endogenous Gcg gene expression. Then, we applied this newly developed Gcg-Cre mouse line to generate a new α-cell-specific Mafb conditional knockout mouse model (MafbΔGcg). Not only α-cell number but also glucagon production were significantly decreased in MafbΔGcg mice compared to control littermates, suggesting an indispensable role of MAFB in both α-cell development and function. Taken together, our newly developed Gcg-Cre mouse line, which was successfully utilized to uncover the role of MAFB in α-cells, is a useful tool for genetic manipulation in pancreatic α-cells, providing a new platform for future studies in this field.

著者

Qing-Shan GAO Mei-Fu XUAN Zhao-Bo LUO Hyo-Jin PAEK Jin-Dan KANG Xi-Jun YIN

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.19-0018, (Released:2019-07-15)

被引用文献数

3

---

The nuclear receptor corepressor Hairless (HR) interacts with nuclear receptors and controls expression of specific target genes involved in hair morphogenesis and hair follicle cycling. Patients with HR gene mutations exhibit atrichia, and in rare cases, immunodeficiency. Pigs with HR gene mutations may provide a useful model for developing therapeutic strategies because pigs are highly similar to humans in terms of anatomy, genetics, and physiology. The present study aimed to knockout the HR gene in pigs using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated-9 system and to investigate the molecular and structural alterations in the skin and thymus. We introduced a biallelic mutation into the HR gene in porcine fetal fibroblasts and generated nine piglets via somatic cell nuclear transfer. These piglets exhibited a lack of hair on the eyelids, abnormalities in the thymus and peripheral blood, and altered expression of several signaling factors regulated by HR. Our results indicate that introduction of the biallelic mutation successfully knocked out the HR gene, resulting in several molecular and structural changes in the skin and thymus. These pigs will provide a useful model for studying human hair disorders associated with HR gene mutations and the underlying molecular mechanisms.

著者

大野 民生 石川 明 山縣 高宏 並河 鷹夫 富田 武

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.41, no.2, pp.123-129, 1992-04-01 (Released:2010-08-25)

参考文献数

8

被引用文献数

9 9

---

行動異常変異形質がスンクスの実験室系統において発見された。行動異常個体は左右両方向への旋回行動と水平方向の首振り行動を特徴としており, 少なくとも生後10日齢において, 明確に判別できた。これら異常個体は生涯, 安定的かつ継続的に異常行動を示した。交配実験により, この行動異常形質は常染色体性の単一遺伝子座の劣性遣伝子により支配され, 浸透度は完全であることが判明した。さらに家系解析により, この遺伝子は沖縄県宜野湾市で捕獲した1匹の野性雄個体に由来することが判明した。そこで, この行動異常形質を「Waltzing」と命名し, 遺伝子記号wzを提唱した。Waltzing個体は旋回行動や首振り行動のほかに, 尾を持って持ち上げると体を頻繁にくねらせたり, 水中で頭部をまったく水面上に維持できない行動を示す。それ故, 一連の異常行動は平衡感覚器の異常と関係があると予想された。しかし, 行動異常個体は, 妊娠期間・産仔数・離乳率および体重についてほぼ正常であり, 毎世代30匹以上から成る閉鎖集団 (WZライン) として育成されている。

著者

Saki TOME Tetsuya SASAKI Satoru TAKAHASHI Yosuke TAKEI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.19-0038, (Released:2019-06-10)

被引用文献数

3

---

T helper 17 (Th17) cells have been suggested to play a crucial role in various complications during pregnancy by participating in maternal immune activation (MIA). To test a possible role for Th17 cells in MIA-mediated abortion, we analyzed transgenic mice overexpressing retinoic acid-related orphan receptor gamma-t (RORγt), a master regulator of IL-17 producing cell development. These mutant mice (RORγt Tg mice) exhibited a constitutive upregulation of serum interleukin-17A (IL-17A) and decreased E-cadherin expression in cell–cell junctions of placental tissues. Abortion after the administration of a viral-mimicking synthetic double-stranded RNA polyinosinic–polycytidylic acid was more frequent in RORγt Tg mice than wild-type mice. These results suggest that excessive Th17 cell activity alters immune responsiveness and increases the rate of abortion during gestation.

著者

Shayida MAIMAITI Ryusuke KOSHIDA Masami OJIMA Kaushalya KULATHUNGA Hisashi OISHI Satoru TAKAHASHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0182, (Released:2019-05-16)

被引用文献数

3

---

Mammalian postnatal growth is regulated primarily by the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis. MafB is a basic leucine zipper (bZip) transcription factor that has pleiotropic functions. Although MafB plays a critical role in fetal brain development, such as in guidance for hindbrain segmentation, its postnatal role in neurons remains to be elucidated. To investigate this, we used neuron-specific Mafb conditional knockout (cKO) mice. In addition to an approximately 50% neonatal viability, the Mafb cKO mice exhibited growth retardation without apparent signs of low energy intake. Notably, serum IGF-I levels of these mice in the postnatal stage were lower than those of control mice. They seemed to have a neuroendocrine dysregulation, as shown by the upregulation of serum GH levels in the resting state and an inconsistent secretory response of GH upon administration of growth hormone-releasing hormone. These findings reveal that neuronal MafB plays an important role in postnatal development regulated by the GH/IGF-I axis.

著者

山口 孝雄 斎藤 徹

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.36, no.2, pp.209-212, 1987-04-01 (Released:2010-08-25)

参考文献数

7

---

自然発情雌に関する交尾未経験雄ハムスターの交尾行動を夜間 (1900~2000時) に赤色ランプ下で30分間観察した。7例中5例の動物に射精に到る一連の交尾行動が見られた。さらに, これらの動物の交尾行動を再び観察したが, 交尾行動の促進は認められなかった。

著者

寺田 英司 中山 太郎 日置 恭司 斎藤 宗雄 奥平 博一

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.29, no.3, pp.365-367, 1980-07-10 (Released:2010-08-25)

参考文献数

7

被引用文献数

2 3

---

Outbred hooded由来であるヌードラット (rnu/rnu) の, 免疫学的性格を検討した。胸腺依存性抗原 (ヒツジ赤血球) に対する抗体産生は, ヘテロ型 (+/rnu) ではIgM, IgG抗体ともに, 産生されるにもかかわらず, ホモ型 (rnu/rnu) ではIgM, IgG抗体とも, 一次反応, 二次反応でも検出されなかった。マウスsarcoma180の移植能を検討したところ, ヘテロ型では腫瘍の生着はみられなかったが, ホモ型では30日間以上生着し, 増殖することがわかった。T細胞にmitogenであるCoA, PHA-Pに対して, ホモ型の脾細胞は反応を示さなかった。以上のことから, rnu遺伝子をホモ型にもつこのヌードラットにおいては, T細胞機能が欠損していると結論した。

著者

Shunichi SUZUKI Daiichiro FUCHIMOTO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0142, (Released:2019-01-14)

被引用文献数

4

---

Tonsils are mucosa-associated lymphoid tissues located at the openings of the gastrointestinal and respiratory tracts, which play a key role in the surveillance of inhaled or ingested pathogens and can concurrently be reservoirs of infectious agents. Therefore, tonsils are important for the immunology and hygiene management of domestic animals, including pigs. However, the process of their fetal developmental has been poorly described, at least in part, because rodents lack tonsils. Therefore, we performed a histological analysis of porcine tonsils of the soft palate from 60 to 100 days of gestation (DG) and from 2 to 14 days post partum (DP). This analysis showed that lymphoid aggregations first appear at DG65, gradually develop during the fetal stage, and expand after birth. In addition, the mRNA expression of chemokine genes involved in lymphoid aggregation and localization was analyzed. CCL19 expression showed the most marked increase and a sharp peak after birth. CCL21 expression changed moderately but showed an interesting bimodal pattern. CXCL13 expression steadily increased throughout the study period. Thus, we demonstrated the mRNA expression of chemokine characteristically changed accompanying tonsillar development.

著者

後藤 一雄 伊藤 豊志雄

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.43, no.3, pp.389-394, 1994

被引用文献数

2

---

ティザー菌をPCR法を用いて, 高感度かつ特異的に検出する目的で, マウス由来ティザー菌 (MSK株) の16SリボゾームDNA (rDNA) の塩基配列を決定し, その配列をRJ株 (ラット由来) の配列と比較した。決定された配列はMSK株およびRJ株で相同性が高かったが (97%以上) , 他の菌種との相同性は低かった (70~83%) ことからティザー菌種に特異的配列を含んでいると考えられた。そこで, PCR法でTyzzer菌種を特異的に検出するプライマーをこれらの塩基配列をもとに選択した。選択されたプライマーを用いたPCR法では, ティザー菌2株 (MSK株およびRJ株) の他, ハムスター由来のHN株を検出できたが, ティザー菌以外の細菌は検出されず, ティザー菌種に対して高い特異性を示した。また, 1個以上のティザー菌 (RJ株) が検出可能であった。さらに, 9匹のJc1: Wistarラットに2×10<SUP>4</SUP>の菌を経口的に実験感染させ, 接種後1, 3および5日目にそれぞれ3匹ずつ肝臓, 心臓, 盲腸, 脾臓および腸間膜リンパ節を採材しPCR法および蛍光抗体法を用いて菌検索を行った。接種後1および3日目の材料からはいずれの方法においても菌は検出されなかった。しかし, 接種後5日目のラットのうち1匹は肝臓および心臓から, 他1匹は盲腸からそれぞれ両方法によって菌が検出された。これらの結果から, 今回明らかにされたMSK株16SrDNAの塩基配列はティザー菌種間でよく保存されていること, さらに選択されたプライマーを用いたPCR法が高感度かつ特異的なティザー菌診断法として有用であることが示唆された。

著者

沢崎 徹 森 裕司 加納 康彦

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.28, no.1, pp.23-38, 1979-01-31 (Released:2010-12-09)

参考文献数

8

---

1.シバヤギに関する形態学的資料を整備する目的で, できる限り自然に近い状態に保定した雄を用い, 断面解剖学的に検討を加えた。2.胸部, 腹部, 骨盤腔部の各臓器, および筋の解剖学的位置関係を明らかにした。3.これらの成績の詳細は, Fig.2~Fig.14に示した。

著者

Abdulsamad Alsalahi Mohammed A. Alshawsh Zamri Chik Zahurin Mohamed

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.67, no.4, pp.517-526, 2018 (Released:2018-11-01)

参考文献数

42

被引用文献数

9

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People consume Catha edulis (khat) for its euphoric effect, and type 1 diabetics have claimed that khat could reduce elevated levels of blood sugar. However, khat has been suggested to provoke diabetes mellitus through destruction of pancreatic β-cells. This study investigated the effect of an ethanolic khat extract on pancreatic functions in type 1 diabetes (T1DM)-induced male Sprague-Dawley rats and to assess its in vitro cytotoxicity in rat pancreatic β-cells (RIN-14B). T1DM was induced in a total of 20 rats with a single intraperitoneal injection of 75 mg/kg of streptozotocin. The rats were distributed into four groups (n=5): the diabetic control, 8 IU insulin-treated, 200 mg/kg khat-treated, and 400 mg/kg khat-treated groups. Another 5 rats were included as a nondiabetic control. Body weight, fasting blood sugar, and caloric intake were recorded weekly. Four weeks after treatment, the rats were sacrificed, and blood was collected for insulin, lipid profile, total protein, amylase, and lipase analysis, while pancreases were harvested for histopathology. In vitro, khat exerted moderate cytotoxicity against RIN-14B cells after 24 and 48 h but demonstrated greater inhibition against RIN-14B cells after 72 h. Neither 200 mg/kg nor 400 mg/kg of khat produced any significant reduction in blood sugar; however, 200 mg/kg khat extract provoked more destruction of pancreatic β-cells as compared with the diabetic control. Ultimately, neither 200 mg/kg nor 400 mg/kg of khat extract could produce a hypoglycemic effect in T1DM-induced rats. However, 200 mg/kg of khat caused greater destruction of pancreatic β-cells, implying that khat may cause a direct cytotoxic effect on pancreatic β-cells in vitro.

著者

Jun IWAMOTO Azusa SEKI Tsuyoshi TAKEDA Yoshihiro SATO Harumoto YAMADA James K. YEH

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.67, no.3, pp.R1, 2018 (Released:2018-07-30)

被引用文献数

1

---

The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.

著者

Jun IWAMOTO Azusa SEKI Tsuyoshi TAKEDA Yoshihiro SATO Harumoto YAMADA James K. YEH

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.67, no.3, pp.R2, 2018 (Released:2018-07-30)

被引用文献数

1

---

The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.