著者

Kotaro Koiwai Kana Morohashi Kazue Inaba Kana Ebihara Hirotatsu Kojima Takayoshi Okabe Ryunosuke Yoshino Takatsugu Hirokawa Taiki Nampo Yuuta Fujikawa Hideshi Inoue Fumiaki Yumoto Toshiya Senda Ryusuke Niwa

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.D20-072, (Released:2021-02-04)

参考文献数

40

被引用文献数

6

---

Insect growth regulators (IGRs) can be developed by elucidating the molecular mechanisms of insect-specific biological events. Because insect molting, and metamorphosis are controlled by ecdysteroids, their biosynthetic pathways can serve as targets for IGR development. The glutathione S-transferase Noppera-bo (Nobo), which is conserved in dipteran and lepidopteran species, plays an essential role in ecdysteroid biosynthesis. Our previous study using 17β-estradiol as a molecular probe revealed that Asp113 of Drosophila melanogaster Nobo (DmNobo) is essential for its biological function. However, to develop IGRs with a greater Nobo inhibitory activity than 17β-estradiol, further structural information is warranted. Here, we report five novel non-steroidal DmNobo inhibitors. Analysis of crystal structures of complexes revealed that DmNobo binds these inhibitors in an Asp113-independent manner. Among amino acid residues at the substrate-recognition site, conformation of conserved Phe39 was dynamically altered upon inhibitor binding. Therefore, these inhibitors can serve as seed compounds for IGR development.

著者

木村 清

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.36, no.1, pp.112-114, 2011-02-25 (Released:2012-11-10)

参考文献数

6

著者

Atsushi Okazawa Takatoshi Wakabayashi Toshiya Muranaka Yukihiro Sugimoto Daisaku Ohta

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.45, no.4, pp.230-237, 2020-11-20 (Released:2020-11-20)

参考文献数

48

被引用文献数

5

---

Orobanchaceae root parasitic weeds cause serious agricultural damage worldwide. Although numerous studies have been conducted to establish an effective control strategy for the growth and spread of root parasitic weeds, no practical method has been developed so far. Previously, metabolomic analyses were conducted on germinating seeds of a broomrape, Orobanche minor, to find novel targets for its selective control. Interestingly, planteose metabolism was identified as a possible target, and nojirimycin (NJ) selectively inhibited the germination of O. minor by intercepting planteose metabolism, although its precise mode of action was unclear. Here, transcriptome analysis by RNA-Seq was conducted to obtain molecular insight into the effects of NJ on germinating O. minor seeds. Differential gene expression analysis results suggest that NJ alters sugar metabolism and/or signaling, which is required to promote seed germination. This finding will contribute to understanding the effect of NJ and establishing a novel strategy for parasitic weed control.

著者

笠原 博幸

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.36, no.4, pp.524-526, 2011-11-25 (Released:2012-11-10)

参考文献数

3

被引用文献数

1

著者

Tetsu Ando Masanobu Yamamoto

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.D20-046, (Released:2020-10-15)

参考文献数

99

被引用文献数

16

---

Since the first identification of bombykol, sex pheromones of about 700 moth species have been elucidated. Additionally, field evaluations of synthetic pheromones and their related compounds have revealed the male attraction of another 1,300 species. These pheromones and attractants are listed on the web-sites, “Pheromone Database, Part I.” Pheromone components are classified according to their chemical structures into two major groups (Types I and II) and miscellaneous. Based on our previous review published in 2004, studies reported during the last two decades are highlighted here to provide information on the structure characteristics of newly identified pheromones, current techniques for structure determination, new enantioselective syntheses of methyl-branched pheromones, and the progress of biosynthetic research. Besides the moth sex pheromones, various pheromones and allomones from many arthropod species have been uncovered. These semiochemicals are being collected in the “Pheromone Database, Part II.” The chemical diversity provides a wonderland for natural product chemists.

著者

伊左治 俊策 吉永 直子 寺石 政義 小川 大輔 加藤 悦子 奥本 裕 土生 芳樹 森 直樹

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.D18-036, (Released:2018-08-03)

参考文献数

29

被引用文献数

1 10

---

イネ(Oryza sativa)は干ばつで枯死するとその後に給水しても復活することはないが,酢酸水溶液を与えたイネは,乾燥ストレスで一度地上部が枯れながらも,再給水すると新たに地上部を伸長させる特異な乾燥耐性を獲得する.処理中の酢酸代謝を解明するために,13Cで標識した酢酸をイネに処理し,LC-MS及び13C-NMRで13C標識された酢酸代謝物を追跡した.根抽出物のLC-MS及び13C-NMRの分析結果から,処理された酢酸はイネ体内に取り込まれ,グルタミン酸デカルボキシラーゼ(GAD)によってγ-アミノ酪酸(GABA)に代謝されると示唆された.酢酸処理したイネのGABA蓄積量を調べると,根に続いて地上部でGABAが蓄積され,また地上部のGABA蓄積量に比例して乾燥ストレスに対する生存率が増加した.以上から,地上部のGABA蓄積が酢酸によるイネの乾燥耐性に寄与する可能性がある.

著者

Motohiro Tomizawa

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.29, no.3, pp.177-183, 2004 (Released:2004-09-07)

参考文献数

63

被引用文献数

13 26

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Neonicotinoids are the only major new class of insecticides introduced in the past three decades. They act as selective agonists at the insect nicotinic acetylcholine receptor and are therefore highly toxic towards important insect pests but relatively safe to mammals. However, the excellent selective toxicity may not be evident with their metabolites or analogous compounds. The aim of this paper is to consider the effects of neonicotinoids and derivatives in mammalian cells and mice involving up-regulation of nicotinic receptor levels and activation of the intracellular signal integration cascade elicited by chronic or sustained exposure and analgesic and toxic effects in mice.

著者

河岸 洋和

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.37, no.4, pp.386-390, 2012-11-20 (Released:2013-05-20)

参考文献数

11

著者

Koji Ito Kazuhiro Takagi Ryota Kataoka Hiromasa Kiyota

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.D20-23, (Released:2020-06-24)

参考文献数

36

被引用文献数

4

---

Nocardioides sp. PD653 genes hcbA1, hcbA2, and hcbA3 encode enzymes that catalyze the oxidative dehalogenation of hexachlorobenzene (HCB), which is one of the most recalcitrant persistent organic pollutants (POPs). In this study, HcbA1, HcbA2, and HcbA3 were heterologously expressed and characterized. Among the flavin species tested, HcbA3 showed the highest affinity for FMN with a Kd value of 0.75±0.17 µM. Kinetic assays revealed that HcbA3 followed a ping-pong bi–bi mechanism for the reduction of flavins. The Km for NADH and FMN was 51.66±11.58 µM and 4.43±0.69 µM, respectively. For both NADH and FMN, the Vmax and kcat were 2.21±0.86 µM and 66.74±5.91 sec−1, respectively. We also successfully reconstituted the oxidative dehalogenase reaction in vitro, which consisted of HcbA1, HcbA3, FMN, and NADH, suggesting that HcbA3 may be the partner reductase component for HcbA1 in Nocardioides sp. PD653.

著者

Arisa Banno Yoshinori Yabuki

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.45, no.1, pp.29-38, 2020-02-20 (Released:2020-02-20)

参考文献数

32

被引用文献数

13

---

This study proposes a practical and precise method for the simultaneous analysis of seven neonicotinoid pesticides in agricultural products using liquid chromatography-tandem mass spectrometry from two different approaches. First, the applicability of a cleanup cartridge, comprised of a polymer sorbent consisting of a styrene-divinylbenzene copolymer with N-containing polar groups and methacrylate, in food samples was demonstrated for the first time. Second, applying an internal standard (IS) calibration method at a lower cost was considered by changing the timing of the IS addition and selecting the minimum number of ISs by referring on the matrix effect. The proposed method resulted in excellent recoveries in all tested matrices (brown rice, grapes, and peanuts) at a spiked concentration of 0.01 mg/kg. Subsequently, a residue analysis of hagobou (young burdock) was conducted. Imidacloprid was detected at 0.02 mg/kg, and the recoveries calculated in parallel with the analysis were satisfactory.

著者

Kazuya Ujihara

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.44, no.4, pp.215-224, 2019-11-20 (Released:2019-11-20)

参考文献数

93

被引用文献数

21

---

The structural modification of natural pyrethrins has led to a number of synthetic pyrethroid insecticides, and each compound has its own characteristics. At present, pyrethroid insecticides are applied not only for household use, the original use for pyrethrins, but also for a wide range of uses such as crop protection, pharmaceuticals, and veterinary applications. Quoting primary sources, this review describes the historical view of structural modifications of pyrethroids, with a focus on structural similarities, and their use.

著者

横山 淳史

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.36, no.3, pp.434-439, 2011-08-25 (Released:2012-11-10)

参考文献数

22

著者

Hisato SUZUKI Kazuo JIKIHARA Masanori SONODA Yoshihiro USUI

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.28, no.2, pp.221-222, 2003 (Released:2004-01-21)

被引用文献数

2 6

著者

Eiki Watanabe Nobuyasu Seike Sayuri Namiki

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.44, no.3, pp.186-191, 2019-08-20 (Released:2019-08-20)

参考文献数

24

被引用文献数

5

---

A micro liquid–liquid extraction has been applied to sample preparation in the current authorized method for clopyralid in compost. The method rendered matrix effects practically negligible during determination with ultraperformance liquid chromatography-electrospray ionization tandem mass spectrometry with an improved limit of quantification of 0.7 µg/kg dry weight. Moreover, it had good accuracy and reproducibility. Therefore, the method is proposed as a highly effective routine analytical technique for investigating the actual status of clopyralid residue in compost.

著者

Kenji Kai

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.J19-02, (Released:2019-07-26)

参考文献数

63

被引用文献数

16

---

Microorganisms produce and secrete a variety of secondary metabolites including fatty acids, polyketides, terpenoids, alkaloids, and peptides. Among them, many molecules act as chemical signals that play important roles in inter-/intra-species microbial communication or the interaction with host organisms. In this review, I focus on our recent reports of the microbial signaling molecules involved in bacterium–fungus, bacterium–plant, and fungus–plant interactions. Their potential contribution to pest management is also discussed.

著者

Atsushi Ishihara Kana Ando Anna Yoshioka Koichi Murata Yu Kokubo Noriko Morimoto Naoki Ube Yukinori Yabuta Makoto Ueno Shin-ichi Tebayashi Kotomi Ueno Kumiko Osaki-Oka

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

pp.D18-063, (Released:2019-03-12)

参考文献数

36

被引用文献数

10

---

We investigated the effect of treatment with hot water extracts from the spent mushroom substrates (SMSs) of Lentinula edodes and Hypsizygus marmoreus on the resistance of rice leaves to Pyricularia oryzae infection. The spraying of the SMS extracts clearly suppressed the development of lesions caused by Py. oryzae infection. The accumulation of phytoalexins momilactones A and B, oryzalexin A, and sakuranetin was markedly induced by the spraying of extracts. The enhanced expression of defense related genes PR1b and PBZ was also found in leaves sprayed with the extracts. Treatments with the extracts also affected phytohormone levels. The levels of N6-(Δ2-isopentenyl)adenine and trans-zeatin markedly increased in response to treatment, whereas the levels of salicylic and jasmonic acids were largely unchanged.

著者

西口 沙也加 村田 晃一 宇部 尚樹 上野 琴巳 手林 慎一 寺石 政義 奥本 裕 森 直樹 石原 亨

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.43, no.3, pp.191-197, 2018-08-20 (Released:2018-08-20)

参考文献数

46

被引用文献数

8

---

イネにおいて1 mM のジャスモン酸を処理することで蓄積量が増加する化合物の探索を行った.その結果,2つの化合物1と2の蓄積量が増加することがわかった.マススペクトルとHPLCにおける保持時間を標準品と比較することで,1と2をそれぞれ13-oxooctadeca-9,11-dienoic acid(13-KODE)および9-oxooctadeca-10,12-dienoic acid(9-KODE)と同定した.これらの化合物の蓄積はイネごま葉枯病菌の感染によっても誘導された.一方で,各KODEをイネの葉に処理すると,抵抗反応に関連する二次代謝産物のサクラネチンやナリンゲニン,セロトニンの蓄積が誘導されたため,これらのKODEが病害応答に関与していることが示唆された.KODEと同じくα,β-不飽和カルボニル構造を持つ化合物について,同様の活性があるか調べたが,KODEの作用は再現されなかった.二次代謝産物の誘導には一定の長さをもった炭素鎖など他の構造因子が必要であると考えられた.

著者

片木 敏行 於勢 佳子

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.40, no.3, pp.69-81, 2015-08-20 (Released:2015-08-20)

参考文献数

176

被引用文献数

6 48

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The toxic effects of pesticides on earthworms, one of the most important bioindicators in the terrestrial environment, are closely related to their body burden determined by uptake, metabolism and excretion processes. Not only the passive diffusion via the outer skin from a dissolved fraction of pesticide but also the ingestion of contaminated soil and food governs the uptake process, with each contribution controlled by either the hydrophobicity of the pesticide or the soil organic matter. Although the available information is limited, earthworms are likely to metabolize pesticides via hydrolysis and oxidation (Phase I) followed by conjugation (Phase II), and low bioaccumulation is observed as a result for most pesticides. The acute toxicity in the soil exposure can be partly explained by the dissolved fraction of pesticide in pore water, but the contribution of dietary uptake and metabolism should be further studied to correctly evaluate pesticide toxicity.

著者

Kenji Kai Keiji Mine Kohki Akiyama Satoshi Ohki Hideo Hayashi

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.43, no.4, pp.283-286, 2018-11-20 (Released:2018-11-20)

参考文献数

18

被引用文献数

10

---

3種のペプタイボール,trichorizin HA II (1), HA V(2)およびHA VI(3)をTrichoderma harzianum HK-61株を植菌したオカラ培養物より単離した.それらの化学構造は,機器分析と化学誘導体化により確定した.Trichorzin類は,キュウリモザイクウイルスがササゲに感染するのをμMオーダーで阻害した.

著者

ラハマン G. K. M. M. 本山 直樹

出版者

Pesticide Science Society of Japan

雑誌

Journal of Pesticide Science (ISSN:1348589X)

巻号頁・発行日

vol.25, no.4, pp.387-391, 2000

被引用文献数

3

---

有機リン殺虫剤クロルピリホスの畑土条件下での残留性を千葉と松戸の黒ボク土を用いて土壌の滅菌処理, 温度 (15, 25, 35℃) や水分 (20, 30, 40%) を変えて室内実験により研究した. 土壌からメタノールで抽出後, 85%リン酸処理によって残留するクロルピリホスを遊離させ, 再びメタノールで抽出した. 遊離したクロルピリホスは千葉土壌で最大18%, 松戸土壌で10%検出されたが, 長期間土壌中に残留した. 土壌中の半減期は千葉土壌で28日, 松戸土壌で14日であり, 温度の上昇に伴って分解は速くなった. メタノールで抽出されない残留体は千葉>松戸であり, 有機炭素含量と正の相関を示し, 土壌有機物への吸着が示唆された. 両土壌とも滅菌処理によって分解速度は遅くなったが, 遊離するクロルピリホスは酸処理によってあまり差がなかった.