著者

高木 昌美 高橋 敏雄 平山 紀夫 Istiyananingsi Mariana Siti Zarkasie Kamaluddin Sumadi 緒方 宗雄 太田 修一

出版者

公益社団法人 日本獣医学会

雑誌

The Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.53, no.4, pp.637-642, 1991

被引用文献数

5

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1987年から1988年にかけて, インドネシア共和国西部ジャワのワクチン未接種の在来鶏(12農場, 196羽)および採卵鶏(8農場, 197羽)を用いて, Haemophilus paragallinarumに対する赤血球凝集抑制抗体の測定を行うと共に, 野外病鶏からの本菌の分離を試み, これらの地域における鶏伝染性コリーザの調査を行った. 本菌に対する抗体は, 地域に関係なく, 在来鶏および産卵鶏から検出された. 供試した農場の70%(14/20), 供試鶏の19%(73/393)に抗体が検出された. A型抗体は, 計11農場(55%)で検出され, 11%(45/393羽)の鶏が陽性を示した. C型抗体は, 計5農場(25%)で検出され, 8%(30/393羽)の鶏が陽性を示した. また, コリーザ様症状を呈する鶏からA型菌2株, C型菌1株の計3株が分離された. これらの成績から, インドネシア国西部ジャワでの, 両血清型による本病の発生が明らかとなった.

著者

高木 昌美 高橋 敏雄 平山 紀夫 Istiyananingsi Mariana Siti Zarkasie Kamaluddin Sumadi 緒方 宗雄 太田 修一

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.53, no.4, pp.637-642, 1991-08-15

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1987年から1988年にかけて, インドネシア共和国西部ジャワのワクチン未接種の在来鶏(12農場, 196羽)および採卵鶏(8農場, 197羽)を用いて, Haemophilus paragallinarumに対する赤血球凝集抑制抗体の測定を行うと共に, 野外病鶏からの本菌の分離を試み, これらの地域における鶏伝染性コリーザの調査を行った. 本菌に対する抗体は, 地域に関係なく, 在来鶏および産卵鶏から検出された. 供試した農場の70%(14/20), 供試鶏の19%(73/393)に抗体が検出された. A型抗体は, 計11農場(55%)で検出され, 11%(45/393羽)の鶏が陽性を示した. C型抗体は, 計5農場(25%)で検出され, 8%(30/393羽)の鶏が陽性を示した. また, コリーザ様症状を呈する鶏からA型菌2株, C型菌1株の計3株が分離された. これらの成績から, インドネシア国西部ジャワでの, 両血清型による本病の発生が明らかとなった.

著者

増田 晃 助川 剛 水本 直恵 谷 浩行 宮本 忠 笹井 知美 馬場 栄一郎

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.62, no.11, pp.1177-1182, 2000-11-25

被引用文献数

4 31

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イヌの外耳炎ではMalassezia pachydermatisが優勢な起炎菌として検出されるが, この真菌の持つ宿主特異性や部位特異性を解明する一助とするため以下の調査と実験を行った.1370頭の来院犬中にみられた120頭の外耳炎症例を対象に, 外耳炎の発生率, 耳翼の形状, 犬種との関係について疫学的調査を行った.垂耳犬種では672頭中85例(12.6%)が, 立耳犬種では698頭中35例(5.0%)が外耳炎であり, 両犬種間には有意差がみられた(P<0.05).脂肪酸定量のための耳垢材料が採取できた95例の耳垢サンプルについて, 培養によりM.pachydermatisを確認した後, 主な脂肪酸をガスクロマトグラフィーにより定量した.M.Pachydermatisの検出率は垂耳犬種で55.2%, 立耳犬種で53.6%と差がなかったが, 総脂肪酸量の平均値は垂耳犬種の方が立耳犬種よりも高く, 立耳犬種でありながら極端に高い脂肪酸値を示したシベリアンハスキーの値を棄却すると両耳型群の間には有意な差(P<0.05)がみられた.42株のM.pachydermatis分離株について脂肪酸の発育増強効果を調べたところ, 大多数の菌株が脂肪酸を利用しながら速く発育することが分かった.以上の結果から、犬種により差はあるものの脂質が多量に分泌されるイヌの耳道内では脂肪酸を好むM.pachydermatisがよく発育し, これはM.pachydermatisがイヌ外耳炎の優勢起炎菌になる理由の一つであると考えられる.

著者

Yingxin ZHANG Zhixuan ZHANG Yinying LOU Yonglan YU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0681, (Released:2021-03-18)

被引用文献数

10

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A year-round molecular epidemiological survey (2017 to 2018) was conducted on three hemoplasmas and two Bartonella species with zoonotic potential in client-owned cats in Beijing and Shanghai. Among 668 specimens, the overall hemoplasma-positive rate was 4.9% (3.4% for Candidatus Mycoplasma haemominutum, 0.9% for Mycoplasma haemofelis and 1.2% for Candidatus Mycoplasma turicensis). The overall Bartonella-positive rate was 8.5% (4.8% for B. henselae and 4.3% for B. clarridgeiae). Age, breed, ectoparasiticide use and stray history, but not city, season and gender, were significantly associated with the positive rates of one or more pathogens. This is also the first report on the prevalence of Candidatus Mycoplasma turicensis in cats in China.

著者

Manabu KOIKE Yasutomo YUTOKU Aki KOIKE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0679, (Released:2021-03-17)

被引用文献数

5

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Radioresistance and radiotoxicity have been reported following cancer treatments in felines. Optimizing radiation doses to induce cytotoxic effects to only cancer cells and not normal cells is critical in achieving effective radiation therapy; however, the mechanisms of radiation resistance, radiotoxicity, and DNA damage response (DDR) in feline cells have not yet been elucidated. A DNA double-strand break (DSB) is the most toxic type of DNA damage induced by X-rays and heavy ion beams used in treating cancers. Crandell-Rees Feline Kidney (CRFK) cells is one of the most widely used cat cells in life science research. Here, we report that DSB-triggered senescence induced by X-rays is important in inhibiting the proliferation of CRFK cells. We demonstrated through cell proliferation assay that X-rays at doses 2 Gy and 10 Gy are toxic to CRFK cells that irradiating CRFK cells inhibits their proliferation. In X-irradiated CRFK cells, a dose-dependent increase in DSB-triggered senescence was detected according to morphological changes and using senescence-associated β galactosidase staining assay. Moreover, our data indicated that in CRFK cells, the major DDR pathway, which involves the phosphorylation of H2AX at Ser139, was normally activated by ATM kinases. Our findings are useful in the understanding of X-rays-induced cellular senescence and in elucidating biological effects of radiation, e.g., toxicity, in feline cells. Furthermore, our findings suggest that the CRFK cell line is an excellent matrix for elucidating radioresistance and radiotoxicity in cat cells.

著者

Aritada YOSHIMURA Takahiro OHMORI Kokoro ITOU Ryo ISHI Yuri MATSUMURA Yuhei WADA Miori KISHIMOTO Tomoko IWANAGA Naoki MIURA Kazuhiko SUZUKI Ryuji FUKUSHIMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0409, (Released:2021-03-15)

被引用文献数

2

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In dogs, pancreatic acinar cell injury is thought to be caused by decreased pancreatic blood flow due to heart failure. In previous our report, it demonstrated that decreased heart function causes a significant decrease in pancreatic blood flow in heart failure dog model caused by rapid ventricular pacing (RVP). However, the types of histopathological changes remain unclear. We aimed to verify the types of histopathological changes occurring in the pancreatic tissue due to decreased heart function. After RVP for 4 weeks, atrophy of pancreatic acinar cells, characterized by a decrease in zymogen granules, was observed in all areas of the pancreas. In conclusion, the result of this study suggests that attention should be paid to ischemia/hypoperfusion injury in the pancreas.

著者

桃沢 幸秀 寺田 節 佐藤 文夫 菊水 健史 武内 ゆかり 楠瀬 良 森 裕司

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.69, no.9, pp.945-950, 2007-09-25

参考文献数

26

被引用文献数

1 18

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ウマの不安傾向を理解することは、騎乗者にとっても獣医師にとっても重要である。本研究では、被験馬をよく知る管理者が気質評価アンケートに回答することで得られた不安傾向スコアと、行動実験から得られた結果とを比較し、不安傾向と関連が深い行動指標を探索した。各馬を新奇環境に導入後、ビニール紐を介して壁に繋留し、管理者が傍にいる状態で2分間観察したのち管理者が離れウマ単独の状態で更に2分間観察した。その結果、単独にされることで多くの観察データが変化したが、アンケート調査により不安傾向が高いと評価された個体ほど、心拍反応が高くビニール紐切断までの潜時は短かった。こうした傾向はメスでより顕著に観察された。以上のことから、新奇環境に単独でおかれた際の心拍数と切断潜時は不安傾向の指標として有効であることが示された。また行動実験と気質評価を組み合わせることで、他の気質項目についてもより信頼性の高い評価を行える可能性が示された。

著者

Tomomi TAKANO Haruna WATANABE Tomoyoshi DOKI Hajime KUSUHARA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0703, (Released:2021-03-11)

被引用文献数

1

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Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.

著者

Makoto AKIYOSHI Masaharu HISASUE Sakurako NEO Masami AKIYOSHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0566, (Released:2021-03-11)

被引用文献数

1

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This report describes the cases of two Miniature Dachshunds who were suspected to have immune-mediated hemolytic anemia (IMHA) and were treated with immunosuppressive therapy. However, progression of anemia, increases in C-reactive protein (CRP) and total-bilirubin (T-Bil) levels, splenomegaly, transition to nonregenerative anemia, and thrombocytopenia occurred after the treatment. Splenectomy and bone-marrow aspirations were performed subsequently. Both dogs were diagnosed with hemophagocytic syndrome (HPS) associated with IMHA. Unfortunately, they died 9 and 6 days later. These findings indicate that some cases of refractory IMHA have the pathogenicity of HPS. HPS should be included as a differential diagnosis of refractory IMHA concurrent with thrombocytopenia. Continuously elevated CRP and T-Bil levels may be helpful indicators in the detection of HPS associated with IMHA.

著者

Takumi KOMIYA Akihiro MORI Naohito NISHII Hitomi ODA Eri ONOZAWA Seri SEKI Toshinori SAKO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.2-0345, (Released:2021-03-05)

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A 5-year-old castrated male domestic shorthair cat was diagnosed with diabetic ketoacidosis and severe insulin resistance. Although the conventional treatment for diabetic ketoacidosis was provided, the cat required frequent hospitalization because of severe dehydration and repeated diabetic ketoacidosis. We detected anti-insulin antibodies for human in this cat. Serum insulin-binding IgG levels were markedly elevated compared with those in healthy cats and other diabetic cats. We initiated prednisolone to suppress the effects of anti-insulin antibodies. After initiation of prednisolone, the cat was gradually recovered with increasing activity and appetite. Furthermore, satisfactory glycemic control was achieved with combined subcutaneous injection of insulin detemir and insulin degludec.

著者

Simona NORMANDO Francesca BERTOMORO Omar BONETTI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0394, (Released:2021-02-23)

被引用文献数

3

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Many dogs are relinquished worldwide, so it is important to enhance adoptions’ success. We aimed at investigating factors associated with owners’ satisfaction with adopted dogs, both in general and focusing on galgos. Data on 392 dogs (191 galgos) were gathered using an online survey, investigating dogs’ and owners’ demographics, satisfaction with the adopted dog and post-adoption behavior. Satisfaction was affected by different variables in galgos’ owners as compared to non-sighthound non-podenco dogs’ ones, with only the presence of disobedience on walks negatively affecting satisfaction in both samples. Depending on dogs’ type, the presence of some behavioral problems was associated with decreased satisfaction with the dog (e.g., destructiveness for galgos, or separation problems for non-sighthound non-podenco dogs), whereas that of others increased it (e.g., not being interested in social interactions with dogs for galgos, and shadowing for non-sighthound non-podenco dogs). The variables most often being predictors of the behaviors influencing satisfaction were dog type,with being a galgo as a negative predictor, and dog’s age, with being older as a negative predictor. Further studies on dog adopters’ satisfaction are needed.

著者

桑野 睦敏 片山 芳也 笠嶋 快周 岡田 幸助 Reilly J.D.

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.64, no.10, pp.893-900, 2002-10-25

参考文献数

25

被引用文献数

2 22

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慢性蹄葉炎では,蹄壁と葉状層の間に黄色調の贅生角質が産生される.贅生角質は大量に産生されると蹄形異常の一要因となるばかりか,蹄真菌症の頻発部位でもあることから,取り除かなければ病態悪化を招く病巣の温床となる.しかしながら,その病態についての研究は全く行われておらず,括削適期すら不明な現状がある.そこで,本研究では,様々な病期の蹄葉炎罹患蹄を用いて,産生される贅生角質の肉眼的な形態変化および組織構築を観察し,贅生角質の形成パターンを調査した.その結果,贅生角質は蹄縦断面では病期約3週間で確認できるようになり,組織学的には異所性の白帯であることがわかった.異所性白帯は,蹄葉炎が治癒しない限り,病期の経過とともに過剰に産生されつづける傾向があった.その括削適期については,まだ十分な量が形成されない発症1ヶ月以内は括削せず,病期1ヵ月を経過した後に括削することが推奨された.

著者

韓 海 渡来 仁 小林 和子 保田 立二

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.59, no.12, pp.1109-1114, 1997-12-25

参考文献数

29

被引用文献数

2

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リポソームを経口ワクチンへ応用するために, pH2.0, 膵液ならびに胆汁存在下でも安定なリポソームの脂質組成を検討し, 経口投与後のIgA抗体産生能について調べた. 経口投与リポソームは, dipalmitoylphosphatidylcholine (DPPC) とcholesterol (Chol) からなるもの, DPPC, dipalmitoylphosphatidylserine (DPPS), Cholからなるもの, distearoylphosphatidylcholine (DSPC) とCholからなるもの, DSPC, DPPS, Cholからなるものを作製し, Tris-HCl buffer (pH2.0), 10%bovine bileならびに2.8%pancreatin液中での安定性を調べた. その結果, DPPC, DPPS, Chol (モル比1:1:2) の脂質組成から作製されたリポソーム, DSPCとChol (モル比7:2) の脂質組成から作製されたリポソームならびにDSPC, DPPS, Chol (モル比7:3:2あるいは1:1:2) の脂質組成から作製されたリポソームは, pH2.0, 10%bile液ならびに2.8%pancreatin液中でも安定であったが, DPPCとChol (モル比7:2) の脂質組成から作製されたリポソームとDPPC, DPPS, Chol (モル比7:3:2) の脂質組成から作製されたリポソームは, pH2.0ならびに10%bile液中で不安定であった. 安定なリポソームのうち, DPPC, DPPS, Chol (モル比1:1:2) の脂質組成からなるリポソームにganglioside GM1を組み込み経口投与し, 血清中のganglioside GM1に対するIgA抗体の産生について調べた. その結果, ganglioside GM1に対する抗体はIgA型抗体のみ産生され, IgG型ならびにIgM型抗体は産生されなかった. さらに, アジュバントとしてmomophosphoryl lipid Aをリポソームに組み込み経口投与した場合, IgA抗体の産生がさらに増強された. 一方, 不安定なリポソームにganglioside GM1を組み込み経口投与した場合には, IgA抗体の産生は誘導されなかった. これらの結果から, 酸性溶液中 (pH2.0), 胆汁中ならびに膵液中で安定なリポソームは, 経口投与によりIgA抗体を効果的に誘導できることが明らかとなり, リポソームを応用した経口ワクチンの開発の可能性が示された.

著者

林 慶 西村 亮平 山木 明 金 輝律 松永 悟 佐々木 伸雄 竹内 啓

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.56, no.5, pp.951-956, 1994-10-15 (Released:2008-02-15)

参考文献数

29

被引用文献数

34 38

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イヌにおいてメデトミジン20μg/kgとミダゾラム0.3mg/kg(Me-Mi), あるいはメデトミジン20μ/kgとブトルファノール0.1mg/kg(Me-B)を組み合わせて投与し, 得られた鎮静効果を, メデトミジン20, 40および80μg/kg(Me20, Me40, Me80)を単独投与した場合の効果と比較検討した. その結果, Me-MiおよびMe-Bでは非常に迅速に強力な鎮静効果が得られ, 約40分間の最大効果発現時には, いずれのイヌも完全に横臥し, 周囲環境, 音刺激に反応せず, 中程度の反射抑制と鎮痛作用が得られ, さらにMe-Miでは自発運動も全く消失し, 優れた筋弛緩作用も得られた. これに対しMe40, Me80では, その鎮静効果はMe-Mi, Me-Bに比べて弱くまた個体間のばらつきもやや大きかった. Me20ではその効果はさらに弱かった. イヌにおいてメデトミジンをミダゾラムあるいはブトルファノールと併用すると, 両者が相乗的に作用することにより低用量のメデトミジンを用いても, 強力な安定した鎮静効果が得られるものと考えられ, とくにメデトミジン-ミダゾラムの組み合わせでは非常に優れた鎮静状態が得られ, イヌの鎮静法として幅広く応用可能で有用であると考えられた.

著者

Daisuke ITO Naoko SHIOZAWA Naoki SEKIGUCHI Chieko ISHIKAWA Nick D. JEFFERY Masato KITAGAWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.6, pp.721-725, 2020 (Released:2020-06-16)

参考文献数

12

被引用文献数

1

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A 30-month-old Maine Coon presented with progressive proprioceptive ataxia, paraparesis, thoracolumbar pain, and decreased appetite. An extradural mass was detected within the left side of the 13th thoracic vertebral canal that compressed the spinal cord on magnetic resonance (MR) and was considered to be mineralized on computed tomography (CT) images. The resected mass was diagnosed as a vertebral vascular hamartoma. Clinical signs improved, but recurrence was diagnosed by MR and CT imaging at 7 months after surgery. Repeated excisional surgery yielded the same diagnosis and the clinical signs abated. Fifteen months after the second surgery, there was apparent vertebral deformation, but there was no further change on CT images by 29 months.

著者

Chayanont BHAMARASUTA Kanyavee PREMRATANACHAI Nitchanan MONGKOLPINYOPAT Pamila YOTHAPAND Thitapa VEJPATTARASIRI Thasinas DISSAYABUTRA Monkon TRISIRIROJ Saikaew SUTAYATRAM Chollada BURANAKARL

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0574, (Released:2021-02-09)

被引用文献数

1

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Iron metabolism, hepcidin and some blood profiles were investigated in 13 healthy and 31 chronic kidney disease (CKD) dogs. The study consisted of 2 experiments, experiment I included healthy dogs (CONT) and CKD dogs (stage 2, 3 and 4), while experiment II consisted of anemic CKD dogs subjected to 28-day darbepoetin alfa treatment. The response to darbepoetin alfa could divide anemic CKD dogs into responder (RP) and non-responder (NRP) subgroups. The results from experiment I showed that packed cell volume (PCV) and plasma albumin concentration were significantly lower in CKD dogs of all stages while the total iron binding capacity (TIBC) was lower in only CKD stage 3 and 4 compared with dogs in CONT group. The PCV was related to both TIBC and albumin when considering among all dogs or only in CKD dogs. The hepcidin concentration in CKD dogs with anemia was lower than those without anemia (P<0.05). In experiment II before darbepoetin alfa treatment, RP subgroup had significantly higher iron and TIBC compared with NRP subgroup (P<0.05), the iron concentration was decreased only in RP subgroup after darbepoetin alfa treatment (P<0.05). The percent increase in PCV was correlated with initial TIBC (P<0.01). Plasma hepcidin concentration was not different between CONT and CKD groups and between RP and NRP subgroups both before and after darbepoetin alfa treatment. It is concluded that TIBC and plasma iron concentration play role on anemia and erythropoietic response to darbepoetin alfa treatment in CKD dogs.

著者

Sung-Yong RHEW Su-Min PARK Qiang LI Ju-Hyun AN Hyung-Kyu CHAE Jung-Hwa LEE Jin-Ok AHN Woo-Jin SONG Hwa-Young YOUN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0195, (Released:2021-02-08)

被引用文献数

5

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Mesenchymal stem cells (MSCs) possess regenerative and immunomodulatory properties and can control the immune dysregulation that leads to β-cell destruction. Stem-cell transplantation could thus manage insulin-dependent diabetes mellitus (IDDM) in dogs. In this pilot study, we aimed to assess canine adipose tissue-derived MSCs (cAT-MSCs) transplantation as a treatment for canine diabetes mellitus. This study included four dogs with over a year of insulin treatment for IDDM, following diagnosis at the Veterinary Medicine Teaching Hospital of Seoul National University. Allogenic cAT-MSCs were infused intravenously three or five times monthly to dogs with IDDM. Blood and urine samples were obtained monthly. General clinical symptoms, including changes in body weight, vitality, appetite, and water intake were assessed. Three of the four owners observed improvement of vitality after stem cell treatment. Two of the four dogs showed improvement in appetite and body weight, polyuria, and polydipsia. C-peptide has increased by about 5–15% in three of the cases, and fructosamine and HbA1c levels have improved in two of the cases. Hyperlipidemia was resolved in two of the dogs, and there was no concurrent bacterial cystitis in any of the dogs. C-peptide secretion and lipid metabolism are associated with diabetic complications. Improvement in these parameters following the treatment suggests that cAT-MSC transplantation in dogs with IDDM might help to improve their insulin secretory capacity and prevent diabetic complications.

著者

Mizuki OGAWA Yasutomo HORI Nobuyuki KANNO Naoki IWASA Takeshi TOYOHUKU Noriko ISAYAMA Akane YOSHIKAWA Ryota AKABANE Atsushi SAKATANI Hirosumi MIYAKAWA Huai-Hsun HSU Yuichi MIYAGAWA Naoyuki TAKEMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0629, (Released:2021-02-05)

被引用文献数

7

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Plasma N-terminal pro-atrial natriuretic peptide (NT-proANP) concentration increases with progression of myxomatous mitral valve disease (MMVD) in dogs. This multicentre, prospective study compared plasma NT-proANP, N-terminal pro-brain natriuretic peptide (NT-proBNP), ANP, and cardiac troponin I (cTnI) concentrations in dogs with MMVD for their characteristics and discriminatory ability to detect cardiac dilatation and congestive heart failure (CHF). Thirty-six healthy dogs and 69 dogs with MMVD were included. Clinical variables were obtained via physical examination, thoracic radiography, and echocardiography. The discriminatory ability of each cardiac biomarker (CB) to determine the presence or absence of cardiac dilatation (event 1) and CHF (event 2) was evaluated using the receiver operating characteristic curves. Plasma NT-proANP, NT-proBNP, and ANP concentrations showed a significant association with the left atrium/aorta ratio (P<0.01). The area under the curve of plasma NT-proANP and NT-proBNP concentrations were 0.72 and 0.75, respectively in event1 and 0.72 and 0.76, respectively in event2. Plasma NT-proANP and NT-proBNP concentrations showed sensitivity 80.0 and 80.0%; specificity 67.6 and 64.7% in event1 (cutoff value; 8,497.81 pg/ml and 1,453.00 pmol/l, respectively) and sensitivity 85.7 and 81.0%; specificity 60.4 and 64.6% in event2 (cutoff value; 8,684.33 pg/ml and 1,772.00 pmol/l, respectively). In dogs with MMVD, plasma NT-proANP, NT-proBNP, and ANP concentrations increase with left atrial enlargement. Particularly, plasma NT-proANP and NT-proBNP concentrations appeared to be equally useful in the discriminatory ability to detect cardiac dilatation and CHF.

著者

Hirokazu ISHINO Iwao SAKONJU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0317, (Released:2021-02-05)

被引用文献数

1

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We analyzed the mRNA expression of matrix metalloproteinases (MMPs), metalloproteinases with thrombospondin motifs (ADAMTSs), and tissue inhibitors of metalloproteinases (TIMPs) in degenerated and herniated intervertebral disks (IVDs) in chondrodystrophic dogs. In degenerated IVDs, MMP3, 7, 13, and 14; ADAMTS4 and 5; and TIMP1–3 expression was significantly higher vs healthy controls (P<0.05). In herniated IVDs, MMP2, 3, 9, 13, and 14; ADAMTS4 and 5; and TIMP1 expression was significantly greater, and MMP7 expression was significantly lower vs degenerated IVDs (P<0.05). These results suggest that metalloproteinase may play a role in extracellular matrix degradation in IVD degeneration. Decreased MMP7 transcription may prevent proteoglycan degradation and reduces macrophage infiltration, which might affect the resorption process of herniated IVDs.

著者

Tommaso VEZZOSI Roberto GHINELLI Paolo FERRARI Francesco PORCIELLO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0622, (Released:2021-02-09)

被引用文献数

3

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This study reports the echocardiographic reference intervals in the American Staffordshire Terrier (AST). The echocardiographic variables obtained in 57 healthy adult AST were compared with published data from the general canine population and other breeds. In the AST, the left ventricular volumes were lower than values reported in Boxers and Dobermans (P<0.0001), but higher than in small breeds (P<0.0001). The left ventricular ejection fraction was higher than Boxers and Dobermans (P<0.0001), but lower than small breed dogs (P=0.027). The aortic peak velocity values were similar to Boxers (P=0.55) but higher than the general canine population (P<0.0001). The reference intervals presented in this study are clinically useful for an accurate echocardiographic interpretation and screening in the AST.