著者

Yanad ABOU MONSEF Osman KUTSAL

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0435, (Released:2021-02-11)

被引用文献数

4

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Hepatic progenitor cells are periportally resident cells capable of differentiating into mature hepatocytes or cholangiocytes to ensure hepatic regeneration. This reaction is termed a ductular reaction. In the present study, regenerative response of the feline liver to different hepatic diseases was investigated immunohistochemically. Regeneration of the liver through hepatocellular replication and proliferation of progenitor cell compartment were comparatively evaluated. Histological and immunohistochemical stainings were conducted on feline liver samples (n=40) representing various hepatobiliary diseases. Cytokeratin (CK) 7, CK19, Proliferating cell nuclear antigen (PCNA), Ki67, and Human hepatocyte marker 1 (Hep Par-1) were used. The presence of progenitor cells within feline livers was proved, both as passive cells in normal liver and as active cells (ductular reaction) in hepatic lesions. CK7 was found to be a suitable antibody for immunohistochemically detecting feline progenitor cells. In acute events, regeneration was predominantly shaped by the division of hepatocytes. In chronic events and severe acute events, hepatocytes lost their ability to divide and regeneration mainly occurred through progenitor cells. Location of the ductular reaction varied between different hepatic diseases. Parenchymal ductular reaction was detected in fulminant hepatitis, chronic hepatitis, hepatocellular lipidosis and metastatic lymphoma, whereas septal ductular reaction was detected in chronic hepatitis and metastatic lymphoma. Ductular reaction exhibited positive staining for Hep Par-1 in chronic and severe acute events. This study indicates the major role played by hepatic progenitor cells in regeneration of the feline liver. Moreover, it shows how the activation pattern of ductular reaction varies according to the hepatobiliary disease type.

著者

落合 和彦 森松 正美 冨澤 伸行 首藤 文榮

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.63, no.10, pp.1103-1108, 2001-10-10

参考文献数

23

被引用文献数

3 27

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小動物臨床の分野において犬の乳腺腫瘍の発症頻度の高さは広く知られるところであり, その詳細な検討が必要とされている.我々は遺伝性乳癌原因遺伝子として知られているBrca2のcDNA, およびBrca2タンパク質と相互作用することが知られているRad51タンパク質のcDNAについて, それぞれの犬ホモログの全長配列をクローニングし, 乳腺腫瘍発症との関わりを検索するための基礎的知見を得ることを目的とした.クローニングしたcDNAは, Brca2が11kb, Rad51が1.5kbで, それぞれ3, 471残基および339残基のアミノ酸をコードすることが推定された.他の動物とアミノ酸配列を比較したところ, Brca2はヒトと68%, マウスと58%の相同性を示した.特にC末端領域の相同性は高く, Rad51結合配列や核移行シグナルと推定されている配列が種をこえて良く保存されていた.また, すでに犬Brca2のゲノム解析により報告されていたexon11との比較から, アミノ酸の置換を伴う多型が存在する可能性が示された.一方, Rad51のアミノ酸配列は, 動物種間の相同性が極めて高かった(ヒトおよびマウスと99%).さらに, 犬のBrca2とRad51の組織分布を調べたところ, 乳腺で両者の発現が見られた.このことはBrca2とRad51が犬でも乳腺でなんらかの相互作用をしている可能性を示すものである.

著者

木下 現 鷲巣 誠 本好 茂一 Breznock Eugene M.

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.57, no.4, pp.703-708, 1995-08-15

参考文献数

31

被引用文献数

2

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循環血液量減少性ショック時の肝血流量の変化を門脈血流量を正確に測定することが出来るよう改良した右心バイパス法を用いて検討した. 安静時の肝臓の血行動態は今までの報告に類似していた. すなわち, 総肝血流量は心拍出量の34%であり, 門脈血流量および肝動脈血流量は総肝血流量のそれぞれ76および24%であった. 血液量減少性ショック時には門脈血流量の減少によって総肝血流量が著しく減少した. この門脈血流量の減少から腸間膜循環はショック中に心拍出量の分配率が低下する末梢循環に分類されることが確認された. ショック中に心拍出量中の肝動脈血流量への分配率が増加した.このことからショック中の肝動脈緩衝反応の発現が確認された. ショック中低下していた総肝血流量はショック状態離脱後の肝動脈血流量の増加によってショック前値にまで回復した. 本試験結果からショック中及びショック状態離脱後の肝血流量減少に対する代償反応として肝動脈緩衝反応の発現が証明された.

著者

澤田 倍美 朴 天鎬 近藤 寿代 森田 剛仁 島田 章則 山根 逸郎 梅村 孝司

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.60, no.7, pp.853-854, 1998-07-25

参考文献数

8

被引用文献数

5 62

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本邦のイヌにおける抗Neospora caninum(NC)抗体保有率を調査した.ネオスポラ症発症および抗NC抗体保有牛飼養農家で飼育されているイヌ48頭中15頭(31.3%)が抗体を保有していた.一方, 都会で飼育されていたイヌ198頭中14頭(7.1%)が抗NC抗体を保有していた.抗体検査2ヵ月前にネオスポラ症が発生したブリーダー宅で飼育されていた7ヵ月齢以上の成犬17頭すべてが抗NC抗体を保有していた.1年半後に同ブリーダーにて再検査を行ったところ, 抗体価に大きな変動はなかった.イヌのブリーダーと酪農家で飼育されていたイヌでNC抗体陽性率が著しく高かったことは, NCがイヌの間で水平伝播し, かつイヌとウシの間で水平伝播されている可能性を示唆するものであった。

著者

Kim Min-Su Nam Tchi-Chou

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.68, no.4, pp.409-411, 2006-04-25

参考文献数

18

被引用文献数

3 22

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脳波検査(EEG)は鎮静または麻酔の評価のための有効な方法である.本研究の目的は,ミニチュアーシュナイザー犬(4.2〜6.1kg,1〜2歳)における針刺激の鎮静効果を脳波検査におけるスペクトルエッジ周波数(SEF)によって調べることである.「GV20と龍会」穴が20分間針刺激された.鎮静レベルは,処置前,処置中および処置後のSEF95値とRamsayの鎮静スコアーによって評価した.GV20または龍会穴の針刺激中,SEF95値は有意に減少し,終了後処置前値に回復した.Ramsayの鎮静スコアーは同様に刺針中適切な鎮静レベルを示した.本研究の結果,犬におけるGV20または龍会穴での針刺激は鎮静を誘導する有用な方法であると結論された.

著者

木村 順平 月瀬 東 岡野 真臣

出版者

公益社団法人 日本獣医学会

雑誌

The Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.54, no.5, pp.1035-1037, 1992

被引用文献数

3

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卵巣性ステロイドホルモンがネコの大前庭腺(バルトリン腺)の分泌の機能にどう関わるかを調べる目的で, 卵巣・子宮を摘出し, エストロジェンまたはプロジェステロン, あるいは両者を投与したネコの大前庭腺の組織切片について, アルシアンブルー, PAS染色ならびに, PNAおよびWGAによるレクチン染色を施し, 組織化学的に検索した. 本実験の結果, ネコ大前庭腺の分泌機能はエストロジェンに依存していることが判明した.

著者

Yasuhiro FUKUYAMA Takuya MARUO Yuta NISHIYAMA Yuki NEMOTO Kaho MURAYAMA Hideki KAYANUMA Shinpei KAWARAI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0670, (Released:2021-01-13)

被引用文献数

3

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The preparation of modified Mohs paste, commonly used for malignant wounds, requires time and effort. Moreover, metal-containing liquid waste is generated when malignant wounds are scrubbed. Therefore, we previously changed the base material of the modified Mohs paste from zinc oxide starch powder to carboxymethyl cellulose (CMC). The novel modified Mohs paste based on CMC (moM-CMC sol) may reduce these disadvantages. In the present study, the moM-CMC sol was applied to malignant tumors in three dogs to manage bleeding and malodor. The moM-CMC sol transitioned into a gel on the tumors within an hour of application and could be easily removed. The symptoms resolved within a few days. The moM-CMC sol could be beneficial for dogs with malignant wounds.

著者

Hyeryoung JEONG Hanbin LEE Jaihyun JUNG Hyunryung KIM Jin YU Hyounglok YOON Youngjae LEE Jinhwa CHANG Dongwoo CHANG

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0703, (Released:2021-01-19)

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Unlike echocardiography, cardiac magnetic resonance imaging (cardiac MRI) results in a near-exact assessment of cardiac structures and function. However, most veterinary studies have focused on dogs with normal cardiac function. We hypothesized that there would be significant differences in cardiac measurements between cardiac MRI and echocardiography when left ventricular (LV) function was abnormal. This study was undertaken to compare measurements of LV function produced by cardiac MRI and echocardiography in dogs whose LV function was altered by pharmacological agents. This study was conducted with six healthy beagle dogs. We increased left ventricular contractility by administration of dobutamine; we decreased cardiac contractility with esmolol. Stroke volume measurements were made by using both cardiac MRI and echocardiography under seven different conditions with general anesthesia: control, three doses of esmolol (100, 200, and 500 µg/kg/min), and three doses of dobutamine (10, 20, and 50 µg/kg/min). Experiments involving each condition were conducted at least 1 week apart. When LV contractility was normal, ejection fraction (EF) and stroke volume (SV), as measured by echocardiography and cardiac MRI, were not significantly different. However, when contractility was changed by pharmacological agents, EF and SV were overestimated by echocardiography, compared to MRI. Evaluation of cardiac function in patients treated with pharmacological agents should be conducted carefully because EF and SV measured by echocardiography can be overestimated, compared with EF and SV obtained by cardiac MRI.

著者

Alessio PIERINI Giada ESPOSITO Eleonora GORI Elena BENVENUTI Pietro RUGGIERO George LUBAS Veronica MARCHETTI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0291, (Released:2021-01-15)

被引用文献数

5

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Few studies have examined platelet alterations in dogs with chronic enteropathy. Our aim was to investigate platelet count (PLT), mean platelet volume (MPV), and platelet-to-lymphocyte ratio (PLR) in dogs diagnosed with immunosuppressant-responsive enteropathy (IRE). In this retrospective study of 41 dogs, data regarding signalment, CCECAI, endoscopic and histopathological scores, PLT, MPV, PLR, total serum protein concentrations, albumin, and iron were collected. Clinical response and relapse were assessed with the evaluation of CCECAI over time. One month after starting therapy, dogs with >25% CCECAI reduction were considered responders. During a three-month CCECAI evaluation as part of a twelve-month follow-up, a CCECAI >3 together with a ≥2 unit increase in responder dogs was considered a relapse. PLT and PLR displayed significant negative correlation with MPV. MPV was positively correlated with total protein and albumin levels and negatively correlated with CCECAI. Three dogs were classified as non-responders, and 14 relapsed within 12 months. No differences were observed in PLT, MPV, or PLR between responding/non-responding and relapsing/non-relapsing groups. PLT, MPV, and PLR correlated with total protein, albumin, and CCECAI, confirming PLT as a potential marker, and suggesting MPV as a new marker of clinical efficacy against canine IRE.

著者

Jun TAMURA Norihiko OYAMA Sho FUKUI Kazuto YAMASHITA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.83, no.1, pp.73-77, 2021 (Released:2021-01-14)

参考文献数

21

被引用文献数

5

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To compare the anesthetic effects after intravenous administration of alfaxalone or propofol without premedication, either alfaxalone (5 mg/kg) or propofol (10 mg/kg) was administered intravenously over 120 sec in 6 cats. Each cat received the alternate treatment at least a 7-day interval. Anesthetic effects (tolerance of intubation, behavior changes and neurodepressive score) and physiological parameters were evaluated. Both treatments produced a rapid loss of consciousness, no apnea, and physiological parameters were maintained within clinically acceptable ranges apart from transient hypoxemia. The degree of hypoxemia was greater after the propofol treatment compared with the alfaxalone treatment. During the recovery period, more adverse events (ataxia, muscular tremors) were observed after the alfaxalone treatment compared with the propofol treatment.

著者

Mayu MUTSUGA James Kenn CHAMBERS Kazuyuki UCHIDA Meina TEI Takao MAKIBUCHI Tatsuya MIZOROGI Akihiko TAKASHIMA Hiroyuki NAKAYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1108250620, (Released:2011-09-02)

被引用文献数

31 53

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The binding of curcumin to senile plaques (SPs) and cerebral amyloid angiopathy (CAA) was examined in the aged brain of various animal species and a human patient with Alzheimer's disease (AD), together with its binding to neurofibrillary tangles (NFTs). Brain sections were immunostained with anti-amyloid β protein 1-42 (Aβ42) and anti-amyloid β protein 1-40 (Aβ40) antibodies. These sections were also stained with alkaline Congo red, periodic acid-methenamine silver (PAM), and curcumin (0.009% curcumin solution) with or without formic acid pretreatment. The sections from the AD brain were also immunostained for anti-paired helical filament-tau (PHF-tau), and were stained with Gallyas silver for NFTs. Some SPs in the AD, monkey, dog, bear, and amyloid precursor protein transgenic mouse (APP Tg-mouse) brains contained congophilic materials, and were intensely positive for curcumin. In addition, curcumin labeled some diffuse SPs negative for Congo red in the AD, monkey, bear, and APP Tg-mouse brains. In all animals, CAA was intensely positive for both Congo red and curcumin. The specific curcumin staining activity was lost by formic acid pretreatment. In the AD brain, NFTs positive for PHF-tau and Gallyas silver were moderately stained with curcumin. These findings indicate that curcumin specifically binds to the aggregated Aβ molecules in various animals, and further to phosphorylated tau protein, probably according to its conformational nature.

著者

Miyuki KOMURA Mayuko SUZUKI Natthanan SANGSRIRATANAKUL Mariko ITO Satoru TAKAHASHI Md. Shahin ALAM Mizuki ONO Chisato DAIO Dany SHOHAM Kazuaki TAKEHARA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.81, no.3, pp.466-472, 2019 (Released:2019-03-30)

参考文献数

39

被引用文献数

1 16

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The inhibitory activities of grapefruit seed extract (GSE) on avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis (SI) and Escherichia coli (EC) were evaluated. Original GSE contained 0.24% benzalkonium chloride (BZC), however, 0.0025% BZC solution could not inactivate bacteria. The activity of diluted GSE (×100, ×500 and ×1,000 with redistilled water) against selected viruses and bacteria was evaluated in this study. The GSE solutions were incubated with the pathogens over a period of time after which the remaining viruses were titrated and the bacterial colonies were counted. In the presence of organic material—5% fetal bovine serum (FBS), the test solutions were sprayed at 1 cm and 30 cm distances to test the efficacy of GSE in a spray form. Furthermore, the efficacy of GSE against bacteria on clothes was tested using non-woven cloth. GSE×100 reduced the viral titer of both AIV and NDV even in 5% FBS condition. IBDV showed high resistance to GSE. GSE×1,000 inactivated both SI and EC within 5 sec, even in the presence of 5% FBS. The disinfectant was able to maintain its efficacy in the spray form at 30 cm distance. GSE was also effective against SI and EC inoculated on fabric. GSE is a potential novel disinfectant against viruses and bacteria, effective even within a short contact time.

著者

Son Hai VU Bomin KIM Alisha Wehdnesday Bernardo REYES Tran Xuan Ngoc HUY John Hwa LEE Suk KIM Hyun-Jin KIM

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0630, (Released:2021-01-19)

被引用文献数

1

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To better understanding Brucella abortus infection, serum metabolites of B. abortus -infected and -uninfected mice were analyzed and twenty-one metabolites were tentatively identified at 3 and 14 days post-infection (d.p.i.). Level of most lysophosphatidylcholines (LPCs) was found to increase in infected mice at 3 d.p.i., while it was decreased at 14 d.p.i. as compared to uninfected mice. In contrast, acylcarnitines were initially reduced at 3 d.p.i then elevated after two-weeks of infection, while hydroxysanthine was increased at 14 d.p.i. in infected mice. Our findings suggest that the significant changes in LPCs and other identified metabolites may serve as potential biomarkers in acute phase of B. abortus infection.

著者

Nana USHINE Aki TANAKA Shin-ichi HAYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.8, pp.1173-1177, 2020 (Released:2020-08-28)

参考文献数

40

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Investigating the cause of animal death is helpful to understand the reasons behind the interactions and conflicts between humans and animals. To further develop the cause of death investigation, we report a case of a Chinese spot-billed duck (Anas zonorhyncha) which hatched from a rescued duck and died 10 days after release. We inspected the duck’s cause of death using an interview of concerned people, external body examination, necropsy, and genetic examinations. Based on the fractures, the main cause of death was determined to be a traffic accident. Furthermore, molecular tests helped to detect raccoon DNA in the visible bite wounds. This case shows that molecular biological method is one of the methods of clarify the animals’ cause of death.

著者

Sakurako NEO Eri MAKIISHI Ayumi FUJIMOTO Masaharu HISASUE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.12, pp.1821-1827, 2020 (Released:2021-01-05)

参考文献数

33

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Long-term culture of canine artificial hepatocytes has not been established. We hypothesized that human placental hydrolysate (hPH) may support the long-term culture of differentiated hepatocyte-like cells. Canine bone marrow cells were cultured using modified hepatocyte growth medium supplemented with hPH. Quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemical analysis for albumin, qualitative RT-PCR for cytochrome P450 1A1 (CYP1A1), hepatocyte growth factor (HGF), Cytokeratin 7 (CK7), CD90, CD44, and CD34, and functional analyses of CYP450 activity and low-density lipoprotein (LDL) uptake were performed. Cultured hepatocyte-like cells were able to maintain hepatocyte characteristics, including morphology, albumin synthesis, CYP450 activity, and LDL uptake for 80 days. Thus, hPH may be a potential facilitator for the long-term culture of hepatocyte-like cells. Clinicopathologically, this culture protocol of artificial hepatocytes will contribute to liver function evaluation.

著者

Yuichi MIYAGAWA Ryota AKABANE Mizuki OGAWA Masayoshi NAGAKAWA Hirosumi MIYAKAWA Naoyuki TAKEMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.12, pp.1828-1834, 2020 (Released:2021-01-05)

参考文献数

34

被引用文献数

6

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Serum cystatin C levels (CysC) are used in human medicine to document progressive kidney failure. Although CysC are not thought to be useful for the diagnosis of kidney dysfunction in dogs, there has been no specific consideration of body weight as a confounding issue. The aim of this study was to assess that the utility of CysC for the diagnosis of decreased glomerular filtration rate (GFR) in smaller vs. larger dogs. In clinically healthy dogs, serum creatinine (Cre) and CysC correlate directly with body weight; we found that dogs weighing <20 kg had significantly lower CysC than those weighing ≥20 kg (0.27 ± 0.07 vs. 0.34 ± 0.05 mg/l, respectively, P<0.001). In dogs weighing <20 kg, CysC had superior diagnostic accuracy for the detection of mildly decreased plasma iohexol clearance (PCio) (<1.8 ml/min/kg) compared with Cre (sensitivity 100% vs. 80.9% and specificity 100% vs. 85.7%); this was not true for dogs weighing ≥20 kg. Additionally, using a cut-off PCio of <1.8 ml/min/kg, the area under receiver-operating characteristics curve (AUC) of CysC was significantly higher than that of Cre in dogs weighing <20 kg (P<0.05); this was not true for dogs weighing ≥20 kg (P=0.695). In conclusion, CysC is a useful marker for the detection of a mild decreasing GFR compared with Cre in dogs weighing <20 kg.

著者

Hitoshi NAKANO Yuki KAMEO Hiroshi SATO Masami MOCHIZUKI Mayumi YOKOYAMA Shigehiko UNI Takahiro SHIBASAKI Ken MAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.71, no.12, pp.1661-1663, 2009 (Released:2009-12-31)

参考文献数

16

被引用文献数

11 12

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Canine distemper virus (CDV) causes a lethal disease among members of the Carnivora. To clarify the distribution of CDV in wild animals, we examined 106 raccoon sera collected from two prefectures in Japan, Hyogo and Osaka, from 2005 to 2007. Among them, 34 raccoons (32.1%) possessed a virus-neutralizing (VN) antibody to KDK-1 strain (genotype Asia-1). There was no significant difference in seroprevalence of CDV regardless of places, gender, and body weights. In Hyogo, a geometric mean of VN titers to KDK-1 was significantly higher than that to Onderstepoort (vaccine strain), indicating that KDK-1-like CDV different from vaccine strain might have spread among raccoon population in Hyogo. In conclusion, CDV is epidemic among feral raccoons in Japan, suggesting that CDV might have been spreading among Japanese wild animals.

著者

Ai HORI Kenjirou MIYOSHI Wakako SEO Ako KAKUTA Kiwamu HANAZONO Tetsuya NAKADE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.12, pp.1763-1768, 2020 (Released:2020-12-26)

参考文献数

20

被引用文献数

1

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We describe a 5-year-old castrated male dog suspected hypoglycemic encephalopathy that was evaluated by using diffusion-weighted imaging (DWI). The dog experienced hypoglycemia after prolonged generalized and continued partial seizures. In the acute phase, DWI showed hyperintensity in the left temporal lobe. After about a month, DWI maintained hyperintensity, and left middle cerebral artery dilation was noted on magnetic resonance angiography (MRA). In the chronic phase, the left temporal lobe lesion was replaced by cerebrospinal fluid. In humans, it was reported that cortical laminar necrosis (CLN) with hypoglycemic encephalopathy presents hyperintensity in the cerebral cortex on DWI and increased vascularity of the middle cerebral artery branches on MRA. In conclusion, DWI has detected CLN in a dog suspected hypoglycemic encephalopathy.

著者

Tadatoshi OHTAKI Haruna FUJIWARA Gen WATANABE Mamiko ONO Kazuyoshi TAYA Shigehisa TSUMAGARI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.12, pp.1773-1780, 2020 (Released:2020-12-26)

参考文献数

39

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The effect of estrus induction by cabergoline on gonadotropin and steroid hormone responses was examined in anestrous bitches. Eleven beagles were used in the study; seven were included in the estrus induction group and four were included in the spontaneous estrus group. Cabergoline was orally administered to the estrus induction group at 5 µg/kg once daily for four weeks, or until hemorrhagic discharge was detected. The inter-estrus interval in the estrus induction group was significantly shorter than the previous estrus interval. Bitches that showed proestrus within four weeks of treatment showed increased luteinizing hormone (LH) pulse frequency and, subsequently, increased estradiol (E2) levels. Prolactin (PRL) levels declined promptly after treatment, except in one bitch that did not show proestrus during the cabergoline treatment period. There was a significant correlation between the time to proestrus induction and the reduction in PRL levels. A positive correlation was found between the LH levels two weeks after cabergoline administration and PRL reduction. This study demonstrates that an abrupt reduction in PRL is likely to be important for initiation of estrus in bitches. A reduction in PRL indirectly leads to an increase in LH pulse frequency, which regulates follicular development in bitches. However, if the period from the end of the previous estrus to the cabergoline treatment is short, it may take some time to show proestrus without increasing E2 levels, even if the LH level increases after cabergoline administration.

著者

Yayoi OTSUKA-YAMASAKI Osamu INANAMI Haruka SHINO Reeko SATO Masahiro YAMASAKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.20-0390, (Released:2020-12-21)

被引用文献数

2

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Hereditary methemoglobinemia associated with nicotinamide adenine dinucleotide-cytochrome b5 reductase (b5R) deficiency is a rare autosomal recessive disorder in animals. Recently, nonsynonymous b5R gene (CYB5R3) variants have been reported to be associated with canine and feline hereditary methemoglobinemia. However, the underlying molecular mechanisms of canine and feline methemoglobinemia caused by these nonsynonymous variants have not yet been reported. Previously, we reported a Pomeranian dog family with hereditary methemoglobinemia, carrying CYB5R3 mutation of an A>C transition at codon 194 in exon 7, replacing an isoleucine residue with leucine (p.Ile194Leu). In this study, we investigated the enzymatic and structural properties of the soluble form of wild-type and Ile194Leu canine b5Rs to characterize the effects of this missense mutation. Our results showed that the kinetic properties of the mutant enzyme were not affected by this amino acid substitution. The secondary structure of the wild-type and Ile194Leu b5Rs detected by circular dichroism showed a similar pattern. However, the mutant enzyme exhibited decreased heat stability and increased susceptibility to trypsin hydrolysis. Moreover, the thermostability and unfolding measurements indicated that the mutant enzyme was more sensitive to temperature-dependent denaturation than the wild-type b5R. We conclude from these results that unstable mutant enzyme properties with normal enzymatic activity would be associated with hereditary methemoglobinemia in the Pomeranian dog family.