著者

Ken Takahata Hiroyuki Miura

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-110, (Released:2016-05-28)

被引用文献数

5

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In the tomato (Solanum lycopersicum), the position of the inflorescence on the stem is known to affect the number of days to first anthesis and has commonly been characterized by the leaf-number (ordinal number from the oldest leaf) of the leaf just below the inflorescence (LEAF-BI) only by the appearance after extension of the stem near the inflorescence. Some examples showing that this evaluation was not suitable were observed by the authors. To confirm the reproducibility of the observation, experiments were conducted in which 4 cultivars were sown in a greenhouse 12 times from Oct. 2008 to Aug. 2010. Based on the vertical positional relationship between the base of the first, second, or third inflorescence and the base of the last initiated leaf before inflorescence primordium on the growing point (L-LEAF, the true guide for determining the inflorescence position), the L-LEAFs of ‘My Lock’ plants in all growth periods and ‘House Momotaro’, ‘Momotaro 8’, and ‘Super Fast’ plants in the non-high-temperature growth periods were always just above the inflorescences, that is, the LEAF-BIs were always the leaves below the L-LEAFs. In contrast, the L-LEAFs of all cultivars except ʻMy Lockʼ in the high-temperature growth periods were often just below the inflorescence, that is, the LEAF-BIs were often the L-LEAFs. Comparing the leaf-number of LEAF-BI and that of L-LEAF demonstrated that the former often overestimated the inflorescence positon among growth periods and cultivars. In temperature treatments with ‘House Momotaro’, such a positional switch of the L-LEAF was reproducible. External observation indicated that the stem on the L-LEAF side and the stem on the inflorescence side extended non-uniformly and the position of the L-LEAF was determined by which side extended faster. Collectively, the LEAF-BI is not a leaf identified morphogenetically, and to identify the position of the inflorescence, the leaf-number of L-LEAF, not LEAF-BI, should be used.

著者

Jammaree Singkaew Shuichi Miyagawa Chalermchai Wongs-Aree Taweerat Vichitsoonthonkul Saneh Sokaokha Songsin Photchanachai

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-087, (Released:2016-05-27)

被引用文献数

6

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Physiological qualities of F1 hybrid tomato seeds affected by the growing season; maturity stage at 40, 45, 50, 60, and 70 days after cross-pollination (DAP), and storage in plastic bags at 0°C for four months were studied. The characteristics of seeds from early harvested fruits (45 DAP) that subsequently ripened at ambient temperature (28 ± 2°C) for ten days (designated as “45+ripening”), were also investigated. Higher light intensity, and a wider range of night and day temperatures and relative humidity (RH), but lower night temperature and RH, were observed in the winter season compared to the rainy season. The winter climate delayed physiological maturity of the seeds by 10 days, indicated by the highest weight of dried seed, percentage germination and germination index which occurred at 60 DAP. On the other hand, in the rainy season physiological maturity occurred at 50 DAP. The proportions of irregularly shaped seeds, abnormal seedlings and weak seedlings were independent of fruit and seed maturity, while the amount of speckled seeds significantly increased with an increase in seed development on either the mother plant or the 45+ripening. Fresh (non-stored seeds) and stored seeds of the fruits harvested at 45+ripening and at 60 DAP showed the same quality. The number of fresh ungerminated seed in the seeds of 45 and 50 DAP was markedly reduced after four months storage at 0°C.

著者

Erasmus Kirii Tanjuro Goto Yuichi Yoshida Ken-ichiro Yasuba Yoshiyuki Tanaka

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-148, (Released:2016-05-27)

被引用文献数

1 21

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Pungency in peppers is due to the presence of the alkaloid capsaicin and its analogues, collectively known as capsaicinoids. These compounds are only produced in the Capsicum genus and function as deterrents to mammals from consuming the pepper fruits. Pungency in pepper is qualitatively controlled by the Pun1 locus, which encodes a putative acyltransferase enzyme. Mutations in the Pun1 gene result in a loss of pungency, and several Pun1 loss-of-function alleles have been identified in sweet peppers to date (pun11–3). However, variations in pun1 alleles have not been completely elucidated. In the present study, we report a new type of loss-of-function pun1 allele, named pun14, in a Japanese sweet pepper cultivar, ‘Nara Murasaki’ (C. annuum). Sequence analysis at the Pun1 locus revealed that this type of Pun1 allele is caused by a single adenine nucleotide insertion in the second exon region. This insertion is unique to ‘Nara Murasaki’ and is not present in wild-type Pun1. This insertion causes a frameshift mutation and a change in the amino acid sequence, resulting in a truncated protein. The results of gene expression analysis indicated that the expression of Pun1 in ‘Nara Murasaki’ was hardly detectable, while the transcripts of this gene were strongly expressed in a pungent cultivar. In a co-segregation test, the pun14 genotype perfectly co-segregated with non-pungency in 103 F2 population plants of a cross between ‘Nara Murasaki’ and a pungent cultivar. ‘Nara Murasaki’ and a DNA marker to distinguish the pun14 allele will be informative for understanding the domestication process of sweet peppers.

著者

Takayuki Sakamoto Motoyasu Ochi Yutaka Kikuchi Kyo Kobayashi Toru Tanaka Yukio Ozaki

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-081, (Released:2016-05-12)

被引用文献数

2

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Harvesting of asparagus spears is hard work because farmers have to harvest spears in a crouching posture. We previously developed electric long-shafted shears and a large-wheeled cart to improve the harvest posture, and we reported that the visibility of spears can be improved by modified branch training using string, without any yield or quality reduction. In the present study, we investigated the harvest efficiency, harvest posture, and subjective burden with electric long-shafted shears and a large-wheeled cart under modified branch training methods (the new standing harvest method) for labor-saving asparagus spear harvesting. Two male subjects in their 30s and 60s harvested spears for 60 min using (standing harvest) and without using (control) the new standing harvest method. The harvest efficiency of the new standing harvest method was 18% lower than that of the control. In the standing harvest, subjects had to pull the shears up to take the harvested spear from the shears with their left hand after each spear was cut (“take”). The subjects needed time for the “take” action, and the time necessary to harvest a spear with the electric long-shafted shears was longer than that required for the control. We also evaluated the harvest posture using the Ovako Working Posture Analyzing System (OWAS). For the subject in his 30s, action category(AC)2 and AC3 accounted for 72% and 14% of the postures recorded in the control, respectively, whereas in the standing harvest, AC1 and AC2 accounted for 57% and 41%, respectively. For the subject in his 60s, AC2 and AC3 accounted for 41% and 58% of the postures recorded in the control, respectively, whereas in the standing harvest, AC1 and AC2 accounted for 27% and 70%, respectively. Unfavorable postures during harvest were reduced by the new standing harvest method. The subjective physical burden on the subjects was evaluated using a modified Borg scale after 60 min of harvesting using and without using the new standing harvest method. The subjective physical burden in both subjects was highest (> 8) at the waist in the control, whereas the scores were lower than 3 in the standing harvest. Thus, the new standing harvest method reduced harvest efficiency, but resulted in substantial improvements in posture and reduced the physical burden on the subjects.

著者

Daiki Matsumoto Ryutaro Tao

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-IR06, (Released:2016-04-27)

被引用文献数

2 43

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Prunus fruit tree species exhibit S-ribonuclease (S-RNase)-based gametophytic self-incompatibility (GSI). This system is also present in the families Plantaginaceae and Solanaceae and the tribe Maleae of the family Rosaceae. In S-RNase-based GSI, self/nonself-recognition between the pistil and pollen is controlled by the pistil S determinant S-ribonuclease gene (S-RNase) and the pollen S determinant F-box gene(s). Accumulated evidence indicates the Prunus pollen S locus contains a single F-box gene, while that of other plants consists of multiple F-box genes. The pollen S F-box genes are called S haplotype-specific F-box (SFB), S-locus F-box brothers (SFBB), and S-locus F-box (SLF) in Prunus, Maleae, and Solanaceae species, respectively. The consequences of pollen S gene mutations and heterodiallelic pollen production differ between Prunus species and other plants, suggesting there are different pollen S functions during self/nonself-recognition. The GSI systems of Prunus and other plants are believed to include the ubiquitin proteasome system for protein degradation. However, Prunus SFB is assumed to facilitate the S-RNase cytotoxic effects during self-recognition, while SLFs and SFBBs are thought to function collaboratively during nonself-recognition to avoid S-RNase cytotoxicity. This review summarizes the distinct features of the S-RNase-based GSI mechanism in Prunus species, with special references to the recent advances in our understanding of S-RNase-based GSI.

著者

Chunfen Zhang Sota Sato Tatsuro Tsukuni Mamoru Sato Hatsuhiko Okada Toshiya Yamamoto Masato Wada Shogo Matsumoto Nobuyuki Yoshikawa Naozumi Mimida Kaori Takagishi Manabu Watanabe Qiufen Cao Sadao Komori

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-094, (Released:2016-04-26)

被引用文献数

3

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To select cultivars to produce doubled haploids (DHs) efficiently in an anther culture, a total of 28 apple cultivars (25 cultivars and 3 rootstock cultivars) were investigated to assess their callus formation rate, embryo formation rate, shoot formation rate from embryo and efficiency of shoot multiplication and acclimatization. The callus formation rates of ‘Red Astrachan’, ‘Kinsei’, ‘Tsugaru’, ‘Golden Delicious’ (‘GD’), ‘American Summer Pearmain’ (‘ASP’), ‘Gala’, ‘Rome Beauty’, and ‘Jonathan’ were high, but ‘JM2’ and ‘King of Tompkins’ formed no callus. Regarding the embryo formation rates, ‘ASP’, ‘Rome Beauty’, ‘M.9’, and ‘Starking Delicious’ (‘SD’) were high, but ‘GD’, ‘JM2’, ‘King of Tompkins’, ‘Ralls Janet’, and ‘Smith’s Cider’ formed no embryo. Regarding the shoot formation rate from embryos, ‘Senshu’, ‘Tsugaru’, and ‘Sansa’ showed high values, but ‘Fuji’, ‘Jonathan’, and ‘Wijcik’ did not form shoots at all. The rate of shoot formation from anthers showed a high–low order of ‘Senshu’, ‘SD’, ‘ASP’, and ‘Tsugaru’. Multiplication and acclimatization of the shoots were not easy. Many individuals died. Eventually, only ‘Senshu’ and ‘SD’ acclimatized. Therefore, cultivars that produced DHs efficiently were ‘Senshu’ and ‘SD’. The results indicated that all processes of “embryo formation”, “shoot induction”, and “shoot multiplication and acclimatization” are important for obtaining DHs in apple anther cultures.

著者

Tomoya Niki Katsutomo Sasaki Masahito Shikata Takako Kawasaki-Narumi Norihiro Ohtsubo Takaaki Nishijima

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-129, (Released:2016-03-08)

被引用文献数

5

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We isolated a torenia mutant “Begonia” from selfed progeny of the mutable line “Flecked,” in which the ventral petal of the flower was converted into dorsal petal. In normal-type (NT) flowers, dorsal petals were pale violet and limbs of lateral and ventral petals were violet, whereas the ventral petal had a yellow nectar guide. In contrast, the ventral petal of mutant-type (MT) flowers changed to pale violet, and the nectar guide disappeared. These altered pigmentation patterns were observed from the early stage of corolla pigmentation. Expression analyses of the floral symmetry genes CYCLOIDEA (CYC), RADIALIS (RAD), and DIVARICATA (DIV) showed that TfCYC1, TfCYC2, TfCYC3, and TfRAD1 were mainly expressed in dorsal petals of NT flowers, but in the mutant, these genes were expressed in the ventral petal similar to dorsal ones. These results suggest that conversion of ventral to dorsal petal in the “Begonia” mutant is caused by high expression of TfCYC1, TfCYC2, TfCYC3, and TfRAD1 in the ventral petal, comparable to their expression in dorsal petals.

著者

Keita Sugiyama Daisuke Kami Takato Muro Takashi Otani Nobuyasu Seike Sayuri Namiki

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-097, (Released:2016-03-01)

被引用文献数

1

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We aimed to characterize the inheritance of HEPX (heptachlor exo-epoxide) uptake ability in summer squash (Cucurbita pepo L.). Crosses between ‘Patty Green’, a cultivar that cannot take up HEPX, and ‘Toyohira 2’, a cultivar that can take up high levels of HEPX, were evaluated in this study. The pattern of inheritance for F1 progeny indicated partial dominance since the measured amount of accumulated HEPX was close to that in ‘Toyohira 2’. In the F2 generation, plants segregated into those that did not take up HEPX and those that did take up HEPX at approximately a ratio of 1:5. This segregation pattern was similar to that for the inhibiting gene (dominant suppression of a dominant allele) in the dihybrid; the expected segregation ratio of 3:13 was supported by a chi-square test. Indeed, the I gene suppresses the N gene (non-transporting gene), but the i gene cannot suppress N (II or Ii suppression of NN or Nn). In this case, the genotype of ‘Patty Green’ is proposed to be iiNN and that of ‘Toyohira 2’ to be IInn. Additionally, we proposed three gene models to explain quantitative variation in HEPX transport. The genotypes of ‘Patty Green’ and ‘Toyohira 2’ are presumed to be ABC and abc, respectively. HEPX cannot be taken up unless two or more different dominant genes are present in a plant. Thus, the genotypes can be divided into HEPX non-transporting (Abc:aBc:abC:abc) and HEPX transporting (ABC:ABc:AbC:aBC) classes. Two or three different dominant genes, irrespective of the gene combination, work together to take up HEPX. In this model, the expected segregation ratio of 10 HEPX non-transporting:54 HEPX transporting was supported by a chi-square test. This pattern of inheritance was also supported by the segregation ratio of self-propagated plants (BC1-s) derived from a backcross. Although both of these inheritance models were correct phenotypically, the function of these genes should be clarified to explain the quantitative differences in HEPX uptake.

著者

Tsuyoshi Habu Hisayo Yamane Ippei Naito Soichiro Nishiyama Ayumi Nonaka Takashi Kawai Hisashi Yamada Ryutaro Tao

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-119, (Released:2016-02-09)

被引用文献数

8

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Japanese persimmon ‘Totsutanenashi’ (TTN) is a spontaneous small fruit mutant derived from ‘Hiratanenashi’ (HTN). To characterize the small fruit phenotype of TTN, we carried out a histological analysis, plant growth regulator treatments, and a transcriptome analysis using Illumina sequencing. The parenchymal cell number in TTN fruit was significantly less than in HTN fruit, and the parenchymal cell size in TTN fruit was also significantly smaller than that in HTN fruit at the later growing stage. However, the fruit size of TTN recovered by cytokinin treatments [50 or 200 ppm N-(2-chloro-4-pyridyl)-N′-phenylurea]. Thus, diminished cytokinin activity in TTN fruits may lead to less cell division in the early growing stage and less cell enlargement in the later growing stage. A large-scale transcriptome analysis was conducted using Illumina sequencing to determine the differences in gene expressions between TTN and HTN fruits. Illumina sequences were processed, resulting in 21,662,190 read pairs from HTN and 23,195,203 read pairs from TTN. After assembly of all sequences from HTN and TTN, 118,985 contigs (referred to as unigenes hereafter) ranging from 201 to 11,954 bases, with an average length of 915 bases, were obtained. Digital expression analyses revealed that the expression levels of 164 unigenes were significantly higher in HTN than in TTN, while the expression levels of 265 unigenes were significantly higher in TTN. A parametric analysis of gene set enrichment using the expression levels of unigenes showed that the biological process Gene Ontology categories of “cell cycle” and “regulation of cell cycle” were significantly down-regulated in TTN. The cell cycle-related differentially expressed genes included D3-type cyclin and Mitogen-Activated Protein Kinase Kinase Kinase. Based on the obtained results, the possible involvement of cell cycle-related genes in regulating the small fruit phenotype in TTN is discussed.

著者

Natsu Tanikawa Hiromichi Inoue Masayoshi Nakayama

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-114, (Released:2016-02-02)

被引用文献数

5

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Flowers of wild Camellia japonica L. are usually red, but infrequently the flowering trees of this species may have purple flowers. Such purple flowers are a highly desired horticultural property, but the color expression is not fixed. Even if a tree has splendid purple flowers in the spring, they may revert back to the red color of a wild C. japonica flower the next year. We investigated the factors responsible for the purple coloration using red, purplish-red, and purple flowers of the cultivar ‘Sennen-fujimurasaki’. The epidermal cells of purplish-red and purple petals were composed of both red and purple colored cells, whereas those of the red petals were uniformly red. Many of the purple cells contained blue-black granules. Cyanidin 3-glucoside and cyanidin 3-p-coumaroylglucoside, major pigments of red-flowered C. japonica, were the major anthocyanins of ‘Sennen-fujimurasaki’. The anthocyanin contents were not noticeably different among flowers of these different colors. Potential co-pigments such as flavones, flavonols, and cinnamic acid derivatives were negligibly detected. No significant differences were found in the Ca, Mg, Mn, Fe, Cu, and Zn ion contents or in the pH of petal homogenates; however, a significant difference was found in the Al ion content. The Al content of the purplish-red and the purple petals was 4–10 times higher and 14–21 times higher than that of red petals, respectively. A cyanidin 3-glucoside solution prepared at pH 4.8 was pale red with no precipitates. When Al ions were added to the cyanidin 3-glucoside solution, the solution became purple and produced blue-black precipitates similar to the blue-black granules observed in the purple colored cells. Differences in the spectral properties of the petals from those of the prepared solution could be caused by the co-occurrence of red and purple cells and may be influenced by other Al-chelating compounds and/or substantial Al concentrations in the vacuoles. We conclude that the purple flower color of ‘Sennen-fujimurasaki’ is generated by chelation of Al ions by anthocyanins. In other purple-flowered C. japonica exhibiting unstable flower coloration similar to that of ‘Sennen-fujimurasaki’, Al-anthocyanin chelation is also likely associated with the purple flower color.

著者

Ryo Norikoshi Katsunori Kohata Hiroko Shimizu-Yumoto Rie Goto Kazuo Ichimura

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-103, (Released:2016-01-13)

被引用文献数

10

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Petal growth associated with flower opening is due to cell expansion. To elucidate the role of soluble carbohydrates in expansion of petal cells in Eustoma grandiflorum, its soluble carbohydrates were identified, and changes in their subcellular concentrations during flower opening were investigated. In addition to glucose, fructose, sucrose, and myo-inositol, d-bornesitol was identified using 1H-NMR. d-Bornesitol was the major soluble carbohydrate in leaves and stems. Given that cyclitols are known to be the translocated carbohydrates in alfalfa, phloem exudate was analyzed. However, the translocated carbohydrate was suggested to be sucrose, and not d-bornesitol. In the petals, glucose and sucrose content increased whereas d-bornesitol and myo-inositol contents were almost constant during flower opening. The fructose content in petals was very low. Glucose, sucrose, myo-inositol, and d-bornesitol were found mainly in the vacuole, although sucrose was also found in the cytoplasm. In the petals of open flowers, glucose and sucrose concentrations in the vacuole increased to 60 and 53 mM. Inorganic ion concentrations in the symplast and apoplast did not increase during flower opening. The osmotic potential of the symplast and apoplast in the petals was lower at the open stage than the potential of those at the bud stage, and this difference was mainly attributed to increases in glucose and sucrose concentrations. The results suggest that the accumulation of glucose and sucrose in the vacuole reduces the symplastic osmotic potential, which appears to be involved in the cell expansion associated with flower opening, but that the contribution of d-bornesitol as an osmoticum to cell expansion is limited in Eustoma.

著者

Hiroshi Yakushiji Atsu Yamasaki Shozo Kobayashi Junko Kaneyoshi Akifumi Azuma Hiroyoshi Sugiura Akihiko Sato

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-080, (Released:2015-12-29)

被引用文献数

4

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‘Hasshu’, a dwarf budsport that originated from the leading persimmon cultivar ‘Hiratanenashi’ (Diospyros kaki Thunb.), was discovered in Japan in 2005. Although ‘Hiratanenashi’ is seedless because of anisoploidy (2n = 135 = 9x), ‘Hasshu’ produces some small normal seeds. In this study, we investigated differences in the morphological characteristics of the vegetative organs and fruits and in the ploidy level between ‘Hasshu’ and ‘Hiratanenashi’. The shoot length, internode length, and leaf size of ‘Hasshu’ were smaller than those of ‘Hiratanenashi’. ‘Hasshu’ bore smaller flowers than those of ‘Hiratanenashi’ in late May, and had consistently smaller fruit than ‘Hiratanenashi’ at all fruit development stages. ‘Hasshu’ ripened in late October, similar to ‘Hiratanenashi’. Both ‘Hiratanenashi’ and ‘Hasshu’ are pollination-variant astringent (PVA) cultivars. Except for the deletion of one allele at ssrdk10, no differences were detected between the two cultivars at four simple sequence repeat (SSR) marker loci. By flow cytometric analysis and chromosome observation, we confirmed that ‘Hasshu’ was octoploid (2n = 120 = 8x), indicating that it was both a dwarf and a ploidy-reduction mutation. These results suggest that recovery of the ability to produce some normal seeds by ‘Hasshu’ may have been caused by the change in ploidy from anisoploid to isoploid.

著者

Taishi Hayashida Kazuyoshi Nada Shin Hiratsuka

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-067, (Released:2015-12-19)

被引用文献数

1

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Since both artificial pollination and fruit thinning are necessary for marketable fruit production of Japanese pears (Pyrus pyrifolia (burm. f.) Nakai) in Japan, about a 20% fruit set is ideal without pollination because only 5% of flowers are actually subjected to fruit production. In this study, copper (Cu2+) and ferrous (Fe2+) ions were shown to be effective for inducing a nearly-ideal fruit set of the Japanese pear ‘Kosui’. Fruit induced by ferrous sulfate (FeSO4) solution or a Bordeaux mixture, which is a combination of copper sulfate (CuSO4), lime, and water, were parthenocarpic, because 1) self-pollen tube growth was not promoted by Cu2+ and Fe2+, 2) almost no perfect seeds were observed at harvest, and 3) Cu2+ and Fe2+ acted as strong inhibitors of pollen tube growth in vitro. The effective stage for inducing parthenocarpy was sprouting time to 4 days after anthesis in the Bordeaux mixture, and sprouting time to 4 days before anthesis in the FeSO4 solution. Annual changes in the effectiveness were found in both chemicals, and the Bordeaux mixture showed no inhibitory effect on the fruit set of cross-pollinated flowers. The growth of Bordeaux mixture-induced fruit was improved by gibberellin (GA) paste or GA paste mixed with N-(N-(2-chloro-4-pyridy1)-N'-phenylurea (CPPU) treatment of the fruit stalk; the treated fruit was about 100 g heavier than the untreated fruit. The GA paste treatment is currently conducted to promote fruit growth and maturation in ‘Kosui’ fruit production in Japan, and the Bordeaux mixture can be substituted for chemical fungicide generally used before anthesis to control scab and black spot disease. Accordingly, the Bordeaux mixture is a promising chemical for great labor-saving in ‘Kosui’ cultivation.

著者

Akiko Ito Daisuke Sakamoto Akihiro Itai Takaaki Nishijima Naomi Oyama-Okubo Yuri Nakamura Takaya Moriguchi Ikuko Nakajima

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-107, (Released:2015-12-17)

被引用文献数

10

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In order to promote increases in the size of ‘Kosui’ Japanese pear [Pyrus pyrifolia (Burm.) Nakai] fruit by plant growth regulators, we applied gibberellin (GA)3+4 paste [2.7% (w/w), A3:A4 = 90:10] in combination with prohexadione-calcium [1%, PCa; BAS-125 (3-oxido-4-propionyl-5-oxo-3-cyclohexene-carboxylate)], an inhibitor of GA 2β-hydroxylation that catabolizes active GA into an inactive form, to fruit pedicels at approximately 30 days after full bloom. GA3+4+PCa treatment advanced fruit growth only in the early stages, but fruit weight did not show any significant differences between the untreated control and GA3+4+PCa-treated fruits at harvest. In contrast, when GA4+7 [2.7% (w/w), A4:A7 = 66:34] was applied, the fruit weight at harvest was greater than that of untreated fruit. Moreover, GA4+7 treatment in combination with PCa resulted in an even higher fruit weight at harvest. The GA4 concentration in fruit flesh was not affected by GA3+4 application at 1 week after the treatment (WAT) either with or without PCa, but GA4 levels increased with GA4+7+PCa treatment, resulting in a significant increase in fruit weight at harvest. A single GA4+7 application almost doubled the GA4 concentration compared with the untreated control, but the difference was not significant. These results indicate that fruit weight at harvest was greater when the GA4 concentration was higher in the fruit flesh at 1 WAT. The higher concentration of GA4 in the GA4+7+PCa-treated fruit compared with the GA4+7 treatment alone may be attributed to the function of PCa that acts to prevent the inactivation of GA4 to GA34 by inhibiting 2β-hydroxylation.

著者

Yoshio Mori Katsuhiko Sumitomo Tamotsu Hisamatsu Tanjuro Goto

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-072, (Released:2015-12-16)

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Summer-to-autumn-flowering small-flowered spray-type (SAFS) chrysanthemums (Chrysanthemum morifolium Ramat.) are commercially important in Japan, and there is an occasional increased demand for these flowers. However, under current cultivation methods, the quality of spray formations is compromised in order to maintain a sufficient production rate at times of peak demand. This study investigated the effect of interrupted lighting (IL), the intercalation of a period of long days (by night interruption) into the natural day length (NDL) period of growing, in order to regulate spray formations in SAFS chrysanthemum cultivars ‘Haruka’ and ‘Subaru’, in which lighting can markedly delay flowering. The effects of the NDL period before IL and those of the IL period were investigated. First, we investigated the effect of 2–12 days of NDL followed by 12 days of IL. Plants subjected to 2–6 days of NDL had longer flower clusters and a greater number of flower buds on the upper lateral flower stems and developed a broom-like spray. Next, we investigated the effect of 4–20 days of IL after 4 days of NDL. Plants subjected to 8–20 days of IL had longer flower clusters, more flower buds on the upper lateral flower stems, and broom-like sprays. These changes were more pronounced when the IL periods were prolonged. Our results showed that spray formation in SAFS chrysanthemum cultivars ‘Haruka’ and ‘Subaru’ can be regulated by controlling the timing and period of IL.

著者

Koji Tanase Takashi Onozaki

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-093, (Released:2015-12-11)

被引用文献数

6

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We investigated differences in flower longevity, ethylene production, and ethylene sensitivity among pot carnation cultivars by quantitative PCR analysis. The flower life of ‘Polaris’ was significantly longer than that of ‘Ariel’ (control cultivar), ‘Orange Duo’, and ‘Lemon Soft’. The flowers of ‘Polaris’ produced little ethylene, and had low expression of the ethylene biosynthesis genes DcACS1 and DcACO1. The transcript levels of senescence-related (SR) genes DcCP1, DcbGal, and DcGST1 increased in petals of ‘Ariel’, ‘Orange Duo’, and ‘Lemon Soft’ at senescence, but were very low in ‘Polaris’. These results suggest that the low ethylene production in ‘Polaris’ is caused by low expression of DcACS1 and DcACO1, as in long-life flowers of the cut carnation cultivars ‘Miracle Rouge’ and ‘Miracle Symphony’. The ethylene sensitivity of ‘Orange Duo’ and ‘Lemon Soft’ was lower than that of both ‘Ariel’ and ‘Polaris’. Petals of ‘Orange Duo’ and ‘Lemon Soft’ wilted, and inrolled more slowly than those of ‘Ariel’ and ‘Polaris’, despite the upregulation of ethylene biosynthesis genes (DcACS1 and DcACO1), DcCP1, DcbGal, DcGST1, and DcEIL3 in petals of all cultivars upon application of exogenous ethylene. These results imply that only the components related to the inrolling of petals are altered in these ethylene-insensitive cultivars.

著者

Hyun Jin Kim Sun Hee Choi Yoon Jin Kim

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-104, (Released:2015-12-10)

被引用文献数

5

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The aims of this study were to examine the effects of different temperatures on seed germination and initial long-day (LD) or short-day (SD) duration on growth, floral initiation, and development of Hosta yingeri S.B.Jones. The germination percentage of H. yingeri seeds was > 90% at 5°C and between 15°C–30°C. The lowest time to the first germination (TFG) and the mean germination time (MGT) were observed at around 30°C. The optimum temperature for germination can be around 30°C considering the final germination percentage, TFG, and MGT together. Plant height, number of leaves, and leaf length increased as the initial SD duration decreased. The percentages of plant bearing buds were 50%, 50%, 20%, 10%, and 0% when the initial SD treatments were 0, 3, 6, 9, and 12 weeks with 29, 26, 23, 20, and 17 weeks of LDs, respectively. No plants flowered when they were exposed to the initial LD conditions. Flower spike length, number of visible buds, and number of open flowers increased as the duration of the initial SD decreased. Four-week-old H. yingeri required ≥ 26 weeks of LD duration to achieve >50% flowering.

著者

Takehiro Suzuki Masumi Yamagishi

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-089, (Released:2015-12-10)

被引用文献数

1 16

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Lilium leichtlinii (2n = 2x = 24) is the most commercially cultivated edible lily in Japan, although viral and fungal diseases are severe problems. Triploid L. lancifolium (2n = 3x = 36), the bulbs of which are also edible, adapts well to the climate in Japan, and feral triploid L. lancifolium plants are often seen along roadsides and in croplands. Thus, triploid L. lancifolium is an important genetic resource for edible lilies, but it develops many bulbils (aerial bulbs) on leaf axils. Bulbil formation is undesirable for edible lilies because bulbils can compete for photosynthate with bulbs, which limits the use of triploid L. lancifolium in commercial production. Edible lily cultivars have been bred by intraspecific crosses within L. leichtlinii, although interspecific hybridization, which increases the level of genetic variation, is the major technique used to breed floricultural lily cultivars. In this study, interspecific-hybrid plants were developed by crossing triploid L. lancifolium and L. leichtlinii, and their characteristics, including bulbil formation ability, were evaluated. The crosses of triploid L. lancifolium × L. leichtlinii developed aneuploid plants, of which the chromosome number ranged from 26 to 34. Bulbil formation ability was continuously distributed in the F1 offspring, although 86% of F1 plants did not develop bulbils, indicating that many aneuploid plants without bulbils are developed by this cross combination. The plants harboring abnormal anthers were segregated in the hybrids. In addition, pollen germination in the F1 hybrids was lower than 20%, and 85% of the hybrids exhibited no pollen germination. However, abnormal anther morphology and low pollen fertility should not be major problems for edible lily production because bulbs are the main commercial output. These results indicate that interspecific hybridization between triploid L. lancifolium and L. leichtlinii has the potential to develop elite edible lily cultivars.

著者

Dao Thi Thanh Huyen Kenji Ureshino Dao Thanh Van Ikuo Miyajima

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-092, (Released:2015-12-04)

被引用文献数

9

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Rhododendron simsii Planch. is distributed in eastern Asia, such as Ryukyu Islands of Japan, southern China, Taiwan, and Vietnam. It is a semideciduous shrub with five-lobed red corollas and reddish-purple blotches on the upper petals. From microscopic observation, reddish-purple colored cells were distributed only in the adaxial subepidermis of blotch areas in the upper petals, while red colored cells were observed in the whole epidermis of both upper and lower petals. Even though the anthocyanin constitutions of upper and lower petals were the same, the λmax of absorbance of fresh upper petals was 514.9 nm, while it was 505.7 nm in the lower petals. In R. simsii flowers, cyanidin glycosides were major anthocyanins in whole petals. Quercetin glycosides were detected in reddish-purple blotch areas, but they were found only in trace amounts in lower petals. Anthocyanins and flavonols were extracted, and separated by column chromatography. Bathochromic shift (9.7 nm) was observed in mixed solution of anthocyanin and flavonol at a ratio of concentration of 1:7.5. These results were similar to the bathochromic shift obtained between upper and lower petals of intact flowers (9.2 nm). From these findings, co-pigmentation between anthocyanin and flavonol (quercetin glycosides) seems to be a key factor of reddish-purple color of the blotch area in the upper petals of R. simsii flowers.

著者

Yuichi Sugihara Hideto Ueno Toshiyuki Hirata Masakazu Komatsuzaki Hajime Araki

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-073, (Released:2015-12-01)

被引用文献数

5

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Utilization of cover crops helps the establishment of environmentally friendly agriculture due to their nutrition supplying ability mainly in the current year of application, but cover crop-derived N also remains until the following year. In the present study, the nutritional effect of a cover crop on tomato production in a greenhouse in the following year was investigated using the 15N-labeling method. Hairy vetch (Vicia villosa R., HV) was used as a cover crop. 15N-labeled HV (1319 mg N/pot) was applied to a 1/2000 a Wagner pot, and a fresh market tomato (Solanum lycopersicum L.), ‘House Momotaro’ was cultivated in it at 0, 80, and 240 kg·ha−1 of N application in 2011 (N0HV, N80HV, and N240HV). After the tomato cultivation in 2011, the soil was stored in a greenhouse (the temperature varied from −4.1°C to 26.5°C) without any water or fertilizer. Tomatoes were cultivated again in the Wagner pots containing the soil used in 2011, to which was added the same rate of N fertilizer (0, 80, and 240 kg·ha−1 of N) and unlabeled HV (935 mg N/pot) in 2012. Total N uptake of tomato plants was higher in N240HV (2377 mg/plant), followed by N80HV (1760 mg/plant), N0HV (1498 mg/plant). On the other hand, the uptake of N derived from HV applied in 2011 (HV2011, 1319 mg N/pot) was not different among the treatments (57.7 mg/plant on average); thus, nitrogen use efficiency derived from HV2011 in 2012 was 4.4% on average. This value was much lower than that in 2011 (47.1% on average), but HV2011-N also remained in the soil after the tomato cultivation in 2012 (500 mg N/pot). The distribution ratios of HV2011-N to the fruit in 1st and 2nd fruit clusters that developed in the early growth period were higher than those of N derived from soil, fertilizer, and HV applied in 2012. These results showed that although the N supplying effect of HV was small, HV could be available not only as short-term N source, but also long-term N source, and HV-derived N applied in the previous year was absorbed by tomato plants during a relatively early growth period in the following year.