著者
松原 武生
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.14, no.6, pp.201-215, 1972-12-25 (Released:2010-09-30)
参考文献数
22
著者
橋本 翼 横山 武司 田中 良和
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.63, no.2, pp.89-96, 2021-05-31 (Released:2021-06-05)
参考文献数
30
被引用文献数
1

Recent marked development called “Resolution revolution” has made cryo-electron microscopy (Cryo-EM) the third method of structure determination at atomic resolution next to X-ray crystallography and NMR. In this review, actual situation surrounding Cryo-EM including an outline about the workflow from sample preparation to image analysis and differences between Cryo-EM analysis and X-ray crystallography is introduced. We hope that this review is useful for researchers particularly who will start Cryo-EM analysis.
著者
福澤 薫 渡邉 千鶴 加藤 幸一郎
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.65, no.1, pp.17-25, 2023-02-28 (Released:2023-03-08)
参考文献数
19

The fragment molecular orbital(FMO)method is a theoretical method that enables quantum chemical calculations of whole bio-macromolecules, such as proteins and nucleic acids, yielding the energies and electron densities of whole molecules as well as interaction energies between fragments. The FMO calculations of protein complexes that have been structurally analyzed experimentally allow for quantitative analysis and physicochemical interpretation of intra- and intermolecular interactions within the complexes. Here we describe the quantitative analysis of structures and interactions using the FMO method, i.e., the “structure and interaction basis” obtained through the complementary use of experimental structure and the FMO calculations.
著者
ネスポロ マッシモ
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.59, no.2-3, pp.51-63, 2017-06-30 (Released:2017-07-01)
参考文献数
16
被引用文献数
4 1
著者
沼本 修孝
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.63, no.3, pp.229-231, 2021-08-31 (Released:2021-09-02)
参考文献数
5

Recent advance in the automatic data collection systems at the synchrotron facilities enable us to obtain high quality diffraction data without on-site operation. Structural determinations from the crystal clusters of small proteins were successfully achieved with microfocus beam, the automated data-collection system, and the data-processing pipeline in SPring-8.
著者
ネスポロ マッシモ
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.58, no.6, pp.251-260, 2016-12-31 (Released:2017-01-06)
参考文献数
15
被引用文献数
5 2
著者
森川 大輔 津田 健治
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.63, no.2, pp.135-142, 2021-05-31 (Released:2021-06-05)
参考文献数
36

Convergent-beam electron diffraction (CBED) is one of the powerful tools for nano-meter scale structural analysis. From its specific futures such as nano-meter probe, dynamical scattering, and direct determination of electrostatic potential, CBED can be applied for space group determination, observation of orbital-ordered state, and local structure analysis. In this paper, some examples for recent results obtained by CBED method are described.
著者
滝沢 由政 胡桃坂 仁志
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.64, no.1, pp.65-68, 2022-02-28 (Released:2022-03-04)
参考文献数
9

Chromatin is a highly organized complex of genomic DNA and proteins in eukaryotic cells. Fundamental unit of chromatin is nucleosome composed of histone octamer and approximately 150 base pairs of DNA. The higher-order conformation of chromatin is involved in important genomic events such as replication, transcription, recombination, and repair. Recently various structures chromatin have been determined by cryo-electron microscopy (cryo-EM). Based on our cryo-EM studies, in this article, we introduce current structures of chromatin determined by cryo-EM.
著者
松垣 直宏 山田 悠介 引田 理英 平木 雅彦 千田 美紀 千田 俊哉
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.62, no.1, pp.56-61, 2020-02-29 (Released:2020-03-06)
参考文献数
21

Applying long wavelength X-ray (ca. longer than 2 Å) in macromolecular crystallography has been limited due to the lack of dedicated measurement environment. Photon Factory BL-1A, as an MX beamline optimized for diffraction experiment using the wavelength from 2.7 to 3.3 Å, offers the opportunity to routinely perform data collection for Native SAD phasing or light atoms identification with much sophisticated manner. Sample optimization is another key for successful experiment at the wavelength where severe absorption is inevitable. We describe how the difficulties in use of long wavelength X-ray were overcome at the beamline and the auxiliary facility, as well as the current results of Native SAD phasing.
著者
熊崎 薫 濡木 理 石谷 隆一郎
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.56, no.4, pp.230-235, 2014-08-31 (Released:2014-09-03)
参考文献数
15

Structural determination of membrane proteins is a key step to understand the molecular mechanisms of the membrane proteins. Lipidic cubic phase (LCP) crystallization is a technique to crystallize the membrane proteins in lipid environment and one of the effective approaches for the membrane protein crystallization. In this article, we describe the overview of crystallization of the membrane proteins in LCP, including the basic principles and procedures of LCP crystallization.
著者
山本 昭二
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.49, no.1, pp.6-11, 2007-02-28 (Released:2010-09-30)
参考文献数
14

Recently a software package for the structure analysis of quasicrystals has been released, giving a better environment for determining guasicrystal structures. Therefore we can analyze their structures if we know data collection and indexing methods and a theory of structure analysis. At this occasion, their structure analysis and its recent development have shortly been reviewed.
著者
平田 秋彦 陳 明偉
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.53, no.5, pp.326-331, 2011-10-31 (Released:2011-11-20)
参考文献数
24

Local structural order in a metallic glass was investigated by Angstrom beam electron diffraction using an aberration corrected scanning transmission electron microscope. The coherent electron probe with a diameter of 0.3 - 0.4 nm was successfully obtained by using Ronchigram method. The fine electron probe enables us to pick up Angstrom-scale local structure information which cannot be obtained by conventional diffraction methods and thereby provide a direct evidence of short- to medium-range order in metallic glasses with the help of ab-initio molecular dynamics simulation.
著者
永嶌 真理子
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.54, no.5, pp.255-262, 2012-10-31 (Released:2012-11-06)
参考文献数
40

Epidote-group minerals are important rock-forming minerals and play a role as a reservoir of transition metal elements, strontium, rare-earth elements and water. They are formed under a variety of geological conditions. Both natural epidotes with complex chemical compositions and synthetic ones with idealized compositions have been investigated to understand their chemico-crystallographical features. In this paper, recent studies on synthetic and natural monoclinic epidotes are reviewed in terms of distributions of cations in coordination polyhedra and structural variations due to cation substitutions. New circumstances such as ‘ghost’ difference Fourier peak as an index of low-crystallinity epidotes and OH-free structure (oxyallanite) are introduced, and subjects for the future studies are proposed.
著者
中津 亨
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.52, no.1, pp.81-88, 2010-02-28 (Released:2011-02-25)
参考文献数
16

Firefly emits visible yellow-green light. The bioluminescence reaction is carried out by the enzyme luciferase. The bioluminescence of luciferase is widely used as an excellent tool for monitoring gene expression, the measurement of the amount of ATP and in vivo imaging. Recently a study of the cancer metastasis is carried out by in vivo luminescence imaging system, because luminescence imaging is less toxic and more useful for long-term assay than fluorescence imaging by GFP. However the luminescence is much dimmer than fluorescence. Then bioluminescence imaging in living organisms demands the high efficient luciferase which emits near infrared lights or enhances the emission intensity. Here I introduce an idea for creating the high efficient luciferase based on the crystal structure.