著者

板屋 民子 飯島 正雄 斉藤 貢一 正木 宏幸 青木 敦子 斎藤 章暢 安藤 佳代子 徳丸 雅一 坂東 正明

出版者

Japanese Society of Food Microbiology

雑誌

食品と微生物 (ISSN:09108637)

巻号頁・発行日

vol.8, no.4, pp.203-212, 1992-03-20 (Released:2010-07-12)

参考文献数

10

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A large number of Photobacterium phosphoreum (6-7 log/g) was isolated from “tamagoyaki” (a kind of nigirisushi; Japanese food) that had been lumineferous in the dark. The isolates were smeared on the surfaces of sliced “tamagoyakis”. After the incubation at 10°C for 48 hr or at 25°C for 24 hr, the surfaces became luminous. It was indicated that this abnormality of “tamagoyaki” was caused by contamination with and multiplication by P. phosphoreum.On the surface of “tamagoyaki”, the bacteria in an early growth phase in such a small number as 4 log/g luminesced. Furthermore, the luminescence was observed when pieces of squid, boiled prawn or “yakichikuwa” (a kind of food made of fishes) with the bacteria were incubated, but not observed on pickled Japanese gizzard shad. Nevertheless the the bacteria grew on the surface of tuna, but no luminescence was observed on it.The bacteria produced a small amount of histamine on squid and tuna (less than 250μg/g), and their ability to putrefy food seemed to be low.The opitmum concentration of sodium chloride for growth of the bacteria in a medium was 3%, but they grew in food containing sodium chloride less than 0.5%. When sodium chloride in the medium was replaced by potassium chloride, calcium chloride, magnesium chloride, ammonium chloride or sodium phosphate, the bacteria were still able to grow but unable to grow when replaced by potassium phosphate or sucrose. The bacteria metabolized arginine by arginine decarboxylase but not by arginine dehydrolase.

著者

湧井 宣行 大久保 哲生 岩崎 雄介 伊藤 里恵 小林 岳 早川 和宏 三井 みゆき 矢野 裕一 斉藤 貢一 中澤 裕之

出版者

一般社団法人日本医療薬学会

雑誌

医療薬学 (ISSN:1346342X)

巻号頁・発行日

vol.37, no.7, pp.425-430, 2011 (Released:2012-08-30)

参考文献数

9

被引用文献数

6 4

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The pulverization of tablets for the preparation of pediatric medicines causes problems with respect to content uniformity and amount of the main ingredient. We compared these 2 factors in a pediatric medicine prepared by 2 methods: tablet grinding and tablet dilution. We also investigated causes of drug loss by means of high-performance liquid chromatography (HPLC).Three pharmacists prepared cortril powder by each method. When the main ingredient content was calculated by quantitative analysis by means of HPLC, there was no significant difference between the 2 methods, and adhesion to the mortar and the package were considered to be major reasons for drug loss.We also examined the effect of the amount of diluent on the loss of the main ingredient in the grinding process, finding that increasing the amount of diluent minimized the loss of the main ingredient content. When the amount of diluent per package was 1.5g, the main ingredient content was 90.8% (n=3).These results suggest that when dispensing small amounts of ground tablets, more attention should be paid to the amount of diluting agents than to the grinding technique.

著者

竹熊 美貴子 大村 厚子 斉藤 貢一

出版者

Society of Indoor Environment, Japan

雑誌

室内環境学会誌 (ISSN:21864314)

巻号頁・発行日

vol.8, no.1, pp.35-39, 2005

被引用文献数

4

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学校施設における室内空気中化学物質濃度の低減化対策として, 換気による効果を確かめる実験を行った。すなわち, 埼玉県内の工業高校を調査対象施設として, 夏季の長期休暇期間中に, 教室内空気中化学物質濃度を換気の前後で測定する方法で行った。<BR>測定した5教室(4普通教室, 1実習室)のうち4教室(3普通教室, 1実習室)から室内濃度指針値を超えるホルムアルデヒドが検出された。その後, 10分間の自然換気を行った普通教室では, ホルムアルデヒド濃度は72%減少し, 室内濃度指針値未満となった。また, アセトアルデヒド濃度も50%減少していた。一方, 窓のない実習室においても, 扇風機を使用した機械換気を行うことでアルデヒド類を低減させることができた。<BR>揮発性有機化合物 (VOC) について, 普通教室では測定対象とした46物質中15~17物質が検出され, 実習室では21物質が検出された。しかし, 換気後, それぞれの教室内気中VOC濃度は換気前に比べて著しく減少し, 2~5物質が検出されたのみであった。この結果, 総揮発性有機化合物 (TVOC) として, 換気により約80~90%の減少であった。

著者

板屋 民子 飯島 正雄 斉藤 貢一 正木 宏幸 青木 敦子 斎藤 章暢 安藤 佳代子 徳丸 雅一 坂東 正明

出版者

日本食品微生物学会

雑誌

食品と微生物 (ISSN:09108637)

巻号頁・発行日

vol.8, no.4, pp.203-212, 1992

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A large number of <I>Photobacterium phosphoreum</I> (6-7 log/g) was isolated from &ldquo;tamagoyaki&rdquo; (a kind of nigirisushi; Japanese food) that had been lumineferous in the dark. The isolates were smeared on the surfaces of sliced &ldquo;tamagoyakis&rdquo;. After the incubation at 10&deg;C for 48 hr or at 25&deg;C for 24 hr, the surfaces became luminous. It was indicated that this abnormality of &ldquo;tamagoyaki&rdquo; was caused by contamination with and multiplication by <I>P. phosphoreum</I>.<BR>On the surface of &ldquo;tamagoyaki&rdquo;, the bacteria in an early growth phase in such a small number as 4 log/g luminesced. Furthermore, the luminescence was observed when pieces of squid, boiled prawn or &ldquo;yakichikuwa&rdquo; (a kind of food made of fishes) with the bacteria were incubated, but not observed on pickled Japanese gizzard shad. Nevertheless the the bacteria grew on the surface of tuna, but no luminescence was observed on it.<BR>The bacteria produced a small amount of histamine on squid and tuna (less than 250&mu;g/g), and their ability to putrefy food seemed to be low.<BR>The opitmum concentration of sodium chloride for growth of the bacteria in a medium was 3%, but they grew in food containing sodium chloride less than 0.5%. When sodium chloride in the medium was replaced by potassium chloride, calcium chloride, magnesium chloride, ammonium chloride or sodium phosphate, the bacteria were still able to grow but unable to grow when replaced by potassium phosphate or sucrose. The bacteria metabolized arginine by arginine decarboxylase but not by arginine dehydrolase.

著者

斉藤 貢

出版者

公益社団法人地盤工学会

雑誌

土と基礎 = Soil mechanics and foundation engineering (ISSN:00413798)

巻号頁・発行日

vol.52, no.11, pp.1-4, 2004-11-01

参考文献数

5

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When technical transfer is considered, the technology must conform to the needs of users. Inappropriate aids such as White Elephant and Cannibalism have been reported due to the mismatch of needs and technologies. Appropriate technology is an important idea, which enhances economic viewpoint. Benefit in terms of economics is defined as willingness to pay, then economic analysis compairs benefit and cost. Technical development means to increase benefit and reduce cost within affordable limit. Global target to halve the proportion without safe water was adopted at UN Millennium Summit. In order to achieve the target, appropriate technology must be employed.

著者

岩崎 雄介 織田 ももこ 佃 優里 永森 裕季 中澤 裕之 伊藤 里恵 斉藤 貢一

出版者

[日本食品衛生学会]

巻号頁・発行日

vol.55, no.4, pp.167-176, 2014 (Released:2014-10-29)

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食品添加物は,保存料,甘味料,着色料,香料など,食品の製造過程または食品の加工・保存の目的で使用されている。個別では安全とうたわれていた食品中の化学物質でも生体内で相互的な複合反応を引き起こし,想定外な影響を与える可能性がある。本研究では食品添加物の安全性を評価するために,酸化防止剤と金属の複合反応に着目し,ラジカルを選択的に検出可能な電子スピン共鳴装置(ESR)を用いた活性酸素種(ROS)生成の評価を行った。酸化防止剤と各種金属を反応させたところ,鉄と銅以外のミネラルや微量金属はROSの産生に寄与しなかった。しかし,特定の酸化防止剤は銅と反応することで,ROSが産生されDNAの酸化と切断を引き起こし,酸化ストレスを惹起させていることが示唆された。

著者

髙橋 美津子 桜井 克巳 菅谷 なえ子 刈込 髙子 斉藤 貢一

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.24, no.1, pp.1-9, 2017 (Released:2017-04-29)

参考文献数

18

被引用文献数

1

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To verify the presence of yohimbine and its four stereoisomers (corynanthine, α-yohimbine, β-yohimbine, and isorauhimbine) in health food and to perform a quantitative analysis, we examined the separation and detection conditions for each component by GC-MS and LC-UV-MS. As a result, in GC-MS analysis, baseline separation of all five components (yohimbine and its four stereoisomers) was achieved with a mid-polar column. However, the presence of a water desorption peak due to heating, calibration curves with poor linearity, and low recovery rates suggested that the use of GC-MS for quantitative analysis required further consideration. On the other hand, in LC-UV-MS analysis, baseline separation of all the five components was possible when a mixture of ammonium bicarbonate buffer and acetonitrile was used as the mobile phase. In addition, calibration curves showed adequate linearity with good recovery rates. On the basis of these results, we decided to employ the analytical condition by LC-UVMS. Using this method, we analyzed commercial health food to determine the actual contents of yohimbine and its stereoisomers, and identified food products in which only yohimbine was detected, as well as those in which yohimbine and its four stereoisomers were detected. Furthermore, we found at a high frequency food products in which corynanthine and α-yohimbine were detected at higher concentrations than yohimbine.

著者

阿久津 守 杉江 謙一 斉藤 貢一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.21, no.1, pp.75-85, 2016 (Released:2016-01-23)

参考文献数

19

被引用文献数

2 4

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In order to prove synthetic cannabinoid abuse, it is necessary to detect intact synthetic cannabinoids or their metabolites from such biological samples as urine or blood. Generally, blood is used as the biological sample because it is usually difficult to detect intact synthetic cannabinoids in urine. Furthermore, a rapid and accurate method for the detection of synthetic cannabinoids in the biological sample is required. Therefore, we examined the applicability of solid-phase dispersive extraction (SPDE)-GC/MS in the rapid detection of intact synthetic cannabinoids in blood. We chose seven synthetic cannabinoids designated as narcotics. To determine the optimum operating conditions for SPDE, we selected Oasis® HLB as the solid-phase material for pre-treatment and filled it with 10 mg into the equipment, and acetone as the eluent. The pre-treatment resulted in 80-100% recovery. Furthermore, the pre-treatment time was significantly reduced in SPDE compared to solid-phase extraction (SPE). In addition, the pre-treatment protected operators from unnecessary exposure, reduced cross-contamination of chemicals, and decreased operation complexity. The limit of detection (S/N>3) of JWH-018, JWH-122, cannabicyclohexanol (CCH), XLR-11, and AM2201 was 2.5 ng/mL, and that of JWH-073 and MAM-2201 was 5 ng/mL. The limit of quantification (S/N>10) of JWH-018, JWH-122, CCH, XLR-11, and AM2201 was 5 ng/mL, and that of JWH-073 and MAM-2201 was 10 ng/mL. The average recoveries of the seven synthetic cannabinoids from pooled serum samples spiked at 25 and 450 ng/mL were 76.9-107.4% (SD: 6.4-10.7%) and 63.1-89.6% (SD: 3.9-8.2%), respectively. (SPDE)-GC/MS was proven to be a useful method for detecting intact synthetic cannabinoids in blood.

著者

坂本 美穂 蓑輪 佳子 岸本 清子 中嶋 順一 鈴木 仁 守安 貴子 深谷 晴彦 斉藤 貢一

出版者

公益社団法人 日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.60, no.4, pp.96-107, 2019-08-25 (Released:2019-08-30)

参考文献数

36

被引用文献数

2

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LC/Tribrid Orbitrapを用いて強壮系健康食品中のphosphodiesterase-5 (PDE-5)阻害薬および構造類似体を迅速かつ確実に同定できる分析法を開発した.液体クロマトグラフの移動相に5 mmol/Lギ酸アンモニウム含有0.1%ギ酸溶液(pH 3)および0.1%ギ酸含有アセトニトリル溶液を使用し,C18カラムでPDE-5阻害薬および構造類似体を分離した後,higher-energy collisional dissociationおよびcollision-induced dissociationの2種類の解離法を用いて複数のMS/MSおよびMS3スペクトルを同時に取得した.本分析法を市販強壮系健康食品および個人輸入医薬品105検体に適用したところ,いずれの検体からもPDE-5阻害薬および構造類似体を検出することができた.さらに,検体から検出された3種類のPDE-5阻害薬の構造類似体と15種類のPDE-5阻害薬および構造類似体の不純物について,本分析法を用いて構造を推定した.本法は強壮系健康食品中に含まれるPDE-5阻害薬および構造類似体の迅速かつ確実な同定および構造推定に有用な方法である.

著者

斉藤 貢一 馬場 奈美季 佐々木 美香 渡邉 みどり 伊藤 里恵 加藤 美穂子 石井 里枝 細江 智夫

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.22, no.3, pp.163-169, 2015

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A simple, rapid, and precise method for the determination of cyclopiazonic acid (CPA) in liquid seasoning was developed, which uses liquid chromatography with ultraviolet and photodiode array detection LC/UV(PDA) and liquid chromatography with time-of-flight mass spectrometry (LC/TOF-MS). CPA in liquid seasoning was subjected to ethyl acetate extraction, and solid-phase extraction using Oasis&reg; HLB. LC/UV(PDA) was used for qualitative and quantitative analysis, and LC/TOF-MS was used as the confirmatory method. The limit of detection (S/N = 3) and the limit of quantification (S/N > 10) of CPA in LC/UV(PDA) were 0.01 μg/mL and 0.03 μg/mL, respectively. Standard materials were prepared for quality control samples (a low concentration of 0.2 μg/mL and a high concentration of 2.0 μg/mL), and internal quality control was performed. As a result, the trueness of the low concentration sample and the high concentration samples was 80.3% and 81.6%, respectively. The relative standard deviation (RSD) of repeatability was 5.3% or less, whereas that of intermediate precision was less than 10.1%. The results suggest that the developed method is useful for the determination of CPA in liquid seasoning.

著者

斉藤 貢一

出版者

星薬科大学

雑誌

基盤研究(C)

巻号頁・発行日

2011

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LC/TOFMSを用いた,痩身薬α-リポ酸のキラル分析法を構築した。本分析法は痩身目的のサプリメント製品の表示違反を判定することが可能となった。DART-TOF-MSによる脱法ハーブ製品中の合成カンナビノイド系および合成カチノン系薬物の迅速スクリーニング法を検討した。本分析法は,大麻との識別も可能であった。乱用の恐れのある医療用薬物として鎮痛薬・鎮痛補助薬30種を選定し、LC/TOFMSを用いた分析法を構築した。前処理に新規に開発した固相分散抽出法を選択することで、除タンパクを必要とせずに試料精製を迅速に行えるようになった。

著者

小濱 純 斉藤 貢一 坂本 裕則 岩崎 雄介 伊藤 里恵 堀江 正一 中澤 裕之

出版者

公益社団法人 日本分析化学会

雑誌

分析化学 (ISSN:05251931)

巻号頁・発行日

vol.56, no.12, pp.1019-1024, 2007 (Released:2008-01-29)

参考文献数

21

被引用文献数

3 5

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食品添加物として使用されているL-プロリンをキラル分離・定量するために,オルトフタルアルデヒド(OPA)と9-フルオレニルメチルクロロホルメート(FMOC-Cl)を使用した選択的な前処理法を検討し,液体クロマトグラフィー/質量分析法によるプロリンの高感度かつ選択的な測定法の構築を試みた.夾雑成分の1級アミノ酸をOPA試薬により誘導体化し,固相抽出カートリッジを用いて2級アミノ酸であるプロリン及びヒドロキシプロリンとの分離を行い,続いて2級アミノ酸をFMOC誘導体とした.この2ステップ誘導体化法により試料のクリーンアップが効果的に行われ,2級アミノ酸に選択的な前処理が可能となった.また,β-シクロデキストリン系のキラルカラムを極性有機相モードで用いることにより,プロリンの良好な光学異性体分離が達成された.清涼飲料水,粉ミルク及び食酢を試料として添加回収試験を行った結果,平均回収率は80∼104% 以内と良好であった.本法は液状食品中のプロリンを光学分離,測定することが可能であり,食品添加物として使用されるプロリンの安全性を確保するための有効な分析法の一つになるものと期待される.