著者

Tomoko FUKUUCHI Makoto YASUDA Katsunori INAZAWA Tatsuhiro OTA Noriko YAMAOKA Ken-ichi MAWATARI Kazuya NAKAGOMI Kiyoko KANEKO

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.29, no.5, pp.511-517, 2013-05-10 (Released:2013-05-10)

参考文献数

31

被引用文献数

6 25

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Several methods for quantifying the purine content in food and drink have been described using high-performance liquid chromatography (HPLC). We have developed an improved HPLC method that is based on a method reported by Kaneko et al. and that is more sensitive yet simple, and suitable for determining the purine content of beer and beer-like alcoholic beverages. Quantitative HPLC separation was performed on a Shodex Asahi Pak GS-320HQ column with an isocratic elution of 150 mmol/L sodium phosphate buffer (H3PO4/NaH2PO4 = 20:100 (v/v)). The retention times for the four analytes, namely, adenine, guanine, hypoxanthine and xanthine, were 19.9, 25.0, 29.3 and 43.0 min, respectively. The resolution was good, and there was no excessive interference from the other compounds in the beverages at these retention times. Furthermore, the detection limit for all the analytes was improved to less than 0.0075 mg/L, and all the calibration curves showed good linearity (r2 > 0.999) between 0.013 and 10 mg/L for adenine and guanine, and between 0.025 and 10 mg/L for hypoxanthine and xanthine. The pretreatment was simplified by removing some procedures and optimizing the perchloric acid hydrolysis and the enzymatic peak-shift assay. We reduced the sample dilution rate by almost 50%, and the time spent on pretreatment from 4 days to only 180 min. The recovery of the analytes from spiked samples was 94.8 – 103.8%. This method may be useful for evaluating quantitative and qualitative differences in the purine content of beer and beer-like alcoholic beverages.

著者

Shinohara Akio Kawada Shin-ichiro Harada Masashi KOYASU Kazuhiro ODA Sen-ichi SUZUKI Hitoshi

出版者

日本哺乳類学会

雑誌

Mammal study (ISSN:13434152)

巻号頁・発行日

vol.33, no.2, pp.77-82, 2008-06-01

被引用文献数

9

著者

Song Chun-Feng Kitamura Yutaka Li Shu-Hong Jiang Wei-Zhong

出版者

Elsevier

雑誌

International journal of greenhouse gas control (ISSN:17505836)

巻号頁・発行日

vol.13, pp.26-33, 2013-03

被引用文献数

34 5

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With the development of CO2 capture technologies, the cryogenic approach provides a promising alternative for greenhouse effect mitigation. In previous work, a novel CO2 capture process was developed based on Stirling coolers (SC). However, the properties of frosted CO2 that deposits onto the heat exchanger of SC are not completely understood but it plays an important role as a heat transfer medium and affects subsequent CO2 deposition. In order to improve the system performance and capture efficiency, a thorough analysis of the CO2 frost process becomes especially significant. Based on this aim, numerical and experimental analysis of CO2 frost formation in the cryogenic capture process has been investigated in the present work. The results show that the frosted CO2 layer significantly influences the heat transfer between the gas stream and cooling fins. Over time (from 0 to 60 min), the thickness of the frost layer increased from 0 to 3.0 mm. The thermal conductivity increased from 0 to 0.4 W/(m K). When the flow rate was set at 1 L/min, the temperature of the cold head of the SC varied from −105.2 °C to −102.1 °C. When the flow rate was 3 L/min, the temperature rose from −106.3 °C to −98.0 °C.

著者

高橋 啓一 間島 信男 野尻湖哺乳類グル-プ

出版者

化石研究会

雑誌

化石研究会会誌 (ISSN:03871924)

巻号頁・発行日

vol.24, no.1, pp.p7-32, 1991-06

被引用文献数

4

著者

Nguyen-Van Triet Hori Noriyuki

出版者

Institution of Engineering and Technology

雑誌

IET Control Theory & Applications (ISSN:17518644)

巻号頁・発行日

vol.7, no.1, pp.80-89, 2013-01

被引用文献数

14 4

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A new approach is proposed for obtaining discrete-time models of a non-linear autonomous continuous-time system based on classification of, what is called in this study, a discrete-time integration gain. The models are expressed as a product of this gain and the system function that has the same structure as that of the continuous-time system. Sufficient conditions on this gain to make the model exact, in the sense defined in this study, are presented. A new discrete-time model is proposed for non-linear systems, which is approximate in general, but exact for linear systems. The method is applicable to any system that has a Jacobian matrix. As examples, van der Pol and Lorenz oscillators are examined and simulated to show that the proposed discrete-time models perform better than other discrete-time models that are known to the authors to be on-line computable, and tends to retain such key features as limit cycles and chaos, even for relatively large sampling periods.

著者

Hitoshi HATAI Mitsuru IBA Daisuke KOJIMA Chun-Ho PARK Yasuhiko TSUCHIDA Toshifumi OYAMADA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.5, pp.639-641, 2013 (Released:2013-05-31)

参考文献数

13

被引用文献数

1 6

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A 13-year-old castrated male Labrador retriever dog presented with a mass caudal to the first molar of his left mandible. Although the tumor was excised, a recurrent tumor was detected one month later and resected. Both tumors displayed invasive growth and were composed of neoplastic proliferation arranged in irregular lobules, nests and cords continuous with mucosal epithelium. The most prominent feature of the tumors was the presence of many proliferating spindle cells admixed with palisading basal-like cells, acanthocytes and stellate cells. In immunohistochemical examinations, the spindle cells were found to be positive for vimentin; cytokeratin AE1/AE3, 5/6, 14 and 19; and p63. The other neoplastic cells were positive for all of these markers shown above except vimentin. Based on these findings, the tumors were diagnosed as spindle cell ameloblastic carcinoma.

著者

Hiroshi Inoue Ken Okumura Hirotsugu Atarashi Takeshi Yamashita Hideki Origasa Naoko Kumagai Masayuki Sakurai Yuichiro Kawamura Isao Kubota Kazuo Matsumoto Yoshiaki Kaneko Satoshi Ogawa Yoshifusa Aizawa Masaomi Chinushi Itsuo Kodama Eiichi Watanabe Yukihiro Koretsune Yuji Okuyama Akihiko Shimizu Osamu Igawa Shigenobu Bando Masahiko Fukatani Tetsunori Saikawa Akiko Chishaki on behalf of the J-RHYTHM Registry Investigators

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-13-0290, (Released:2013-05-25)

参考文献数

24

被引用文献数

52 110

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Background: Target anticoagulation levels for warfarin in Japanese patients with non-valvular atrial fibrillation (NVAF) are unclear. Methods and Results: Of 7,527 patients with NVAF, 1,002 did not receive warfarin (non-warfarin group), and the remaining patients receiving warfarin were divided into 5 groups based on their baseline international normalized ratio (INR) of prothrombin time (≤1.59, 1.6–1.99, 2.0–2.59, 2.6–2.99, and ≥3.0). Patients were followed-up prospectively for 2 years. Primary endpoints were thromboembolic events (cerebral infarction, transient ischemic attack, and systemic embolism), and major hemorrhage requiring hospital admission. During the follow-up period, thromboembolic events occurred in 3.0% of non-warfarin group, but at lower frequencies in the warfarin groups (2.0, 1.3, 1.5, 0.6, and 1.8%/2 years for INR values of ≤1.59, 1.6–1.99, 2.0–2.59, 2.6–2.99, and ≥3.0, respectively; P=0.0059). Major hemorrhage occurred more frequently in warfarin groups (1.5, 1.8, 2.4, 3.3, and 4.1% for INR values ≤1.59, 1.6–1.99, 2.0–2.59, 2.6–2.99, and ≥3.0, respectively; P=0.0041) than in non-warfarin group (0.8%/2 years). These trends were maintained when the analyses were confined to patients aged ≥70 years. Conclusions: An INR of 1.6–2.6 is safe and effective at preventing thromboembolic events in patients with NVAF, particularly patients aged ≥70 years. An INR of 2.6–2.99 is also effective, but associated with a slightly increased risk in major hemorrhage. (UMIN Clinical Trials Registry UMIN000001569)

著者

Jin-Hun Sohn Estate Sokhadze Shigeki Watanuki

出版者

日本生理人類学会

雑誌

Journal of PHYSIOLOGICAL ANTHROPOLOGY and Applied Human Science (ISSN:13453475)

巻号頁・発行日

vol.20, no.2, pp.55-64, 2001 (Released:2001-06-12)

参考文献数

59

被引用文献数

6 15

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The purpose of this paper is to analyze the current state of developmental researches in the area of psychophysiology of emotions in preschool and elementary school children. Electrodermal and cardiovascular activity measures are considered as the sources of indices of the autonomic nervous system activation during emotion-eliciting stimulation in children. We discuss the question of sensitivity of phasic and tonic autonomic measures for the identification of occurrence of emotion, mapping it along with valence and arousal dimensions in affective space, and to further differentiate emotions by their physiological manifestations. Considered are the conceptual and methodological issues related to psychophysiological measurements and developmental factors affecting the emotional reactivity in children. Special attention is devoted to the developmental aspects of psychophysiological studies on emotion such as the maturation of organs, integration of the autonomic and central nervous systems, age and gender-related changes in autonomic reactivity, and development of inhibitory control. Summarized are main findings relevant to psychophysiology of emotions in preschool and early school-age children and suggested are most perspective directions of their integration in the framework of modern theories of emotion.

著者

Hun-Young Yoon Soon-Wuk Jeong

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0464, (Released:2013-05-27)

被引用文献数

7

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We report here the clinical presentation, magnetic resonance imaging (MRI) findings and successful surgical management associated with triceps tendonavulsionin a dog.A definitive diagnosis of triceps tendon avulsion was made based on MRI with evidence ofdisplacement of the triceps tendon. Surgical correction oftriceps tendon avulsion was performed with two horizontal mattress sutures using polyester and two tunnels drilled in the olecranon to reattach the tendon to the proximal olecranon. At 9 months, there was no evidence of lameness on the left thoracic limb. This is the first case report to describe MRI evaluation for the diagnosis of the triceps tendon avulsion.

著者

Benseman T. M. Koshelev A. E. Kwok W.-K. Welp U. Vlasko-Vlasov V. K. Kadowaki K. Minami H. Watanabe C.

出版者

American Institute of Physics

雑誌

Journal of applied physics (ISSN:00218979)

巻号頁・発行日

vol.113, no.13, pp.133902, 2013-04

被引用文献数

55

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Stacks of intrinsic Josephson junctions (IJJs) made from high-temperature superconductors such as Bi2Sr2CaCu2O8+δ (Bi-2212) (BSCCO) are a promising source of coherent continuous-wave terahertz radiation. It is thought that at electrical bias conditions under which THz-emission occurs, hot spots may form due to resistive self-heating, and that these spots may be highly beneficial for the generation of high levels of THz power. Here, we perform an imaging study of the temperature distribution at the surface of BSCCO stacks utilizing the temperature-dependent 612 nm fluorescence line of Eu3+ in a europium chelate. The images directly reveal a highly non-uniform temperature distribution in which the temperature in the middle of the stack can exceed the superconducting transition temperature by tens of Kelvin under biasing conditions typical for THz-emission.

著者

Jun-ichi Oyama Toyoki Maeda Makoto Sasaki Kazuya Kozuma Ryuji Ochiai Ichiro Tokimitsu Sachiyo Taguchi Yoshihiro Higuchi Naoki Makino

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.49, no.23, pp.2553-2559, 2010 (Released:2010-12-01)

参考文献数

37

被引用文献数

13 32

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Objective Several epidemiological investigations have reported that green tea reduces cardiovascular and cerebral vascular risks. Green tea catechins may improve peripheral endothelial dysfunction in smokers. The purpose of this study was to elucidate the beneficial effect of green tea catechins on the repair of endothelial dysfunction in smokers. Methods Thirty healthy male smokers divided into three groups ingested a green tea beverage containing 0 mg (control group), 80 mg (middle dose group) or 580 mg (high dose group) of green tea catechins (GTC) daily for two weeks, and endothelial-dependent vasodilatation was investigated by measuring forearm blood flow (FBF) response to reactive hyperemia (RH) by venous occlusion strain-gauge plethysmography. Results An acute effect was that the FBF response to RH significantly increased 2 hr after GTC intake in the high dose group. However, no increase was observed in the other groups. The chronic administration of GTC for one or two weeks ameliorated the FBF responses to RH in the high dose group. On the other hand, no significant increase was observed in the FBF responses to RH in the other groups. Moreover, the plasma concentration of 8-OHdG, IL-6, TNF-alpha, and soluble Fas decreased significantly for two weeks in the high dose group, however, the level of IL-1 beta remained unchanged over this period. Conclusion Green tea consumption over short and long periods appears to ameliorate endothelial dysfunction by scavenging free radicals with anti-inflammatory and anti-apoptotic properties in healthy male smokers.

著者

Marie S. Møller Darrell Cockburn Jonas W. Nielsen Johanne M. Jensen Malene B. Vester-Christensen Morten M. Nielsen Joakim M. Andersen Casper Wilkens Julie Rannes Per Hägglund Anette Henriksen Maher Abou Hachem Martin Willemoës Birte Svensson

出版者

The Japanese Society of Applied Glycoscience

雑誌

Journal of Applied Glycoscience (ISSN:13447882)

巻号頁・発行日

pp.jag.JAG-2012_023, (Released:2013-03-21)

被引用文献数

1 1

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Certain enzymes interact with polysaccharides at surface binding sites (SBSs) situated outside of their active sites. SBSs are not easily identified and their function has been discerned in relatively few cases. Starch degradation is a concerted action involving GH13 hydrolases. New insight into barley seed α-amylase 1 (AMY1) and limit dextrinase (LD) includes i) kinetics of bi-exponential amylopectin hydrolysis by AMY1, one reaction having low Km (8 µg/ml) and high kcat (57 s-1) and the other high Km (97 µg/ml) and low kcat (23 s-1). β-Cyclodextrin (β-CD) inhibits the first reaction by binding to an SBS (SBS2) on domain C with Kd = 70 µM, which for the SBS2 Y380A mutant increases to 1.4 mM. SBS2 thus has a role in the fast, high-affinity component of amylopectin degradation. ii) The N-terminal domain of LD, the debranching enzyme in germinating seeds, shows distant structural similarity with domains including CBM21 present in other proteins and involved in various molecular interactions, but no binding site identity. LD is controlled by barley limit dextrinase inhibitor (LDI) which belongs to the cereal-type inhibitor family and forms a tight 1:1 complex with LD. iii) LDI in turn is regulated by disulfide reduction mediated by the barley thioredoxin h (trxh) NADPH-dependent thioredoxin reductase (NTR) system. Based on the progress monitored by released free thiol groups from LDI and its failure to inhibit LD as elicited by trxh, the LDI inactivation is proposed to stem from loss of structural integrity due to reduction of all four disulfides.

著者

Jae Hyun BAE Yong Hwan KWON Ye Chan JUNG Ji Mo JUNG Hae Beom LEE Ki Chang LEE Nam Soo KIM Min-Su KIM

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0146, (Released:2013-05-14)

被引用文献数

4

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A 2-year-old male mixed dog was referred to us for further evaluation and treatment of a 4-week-history of oliguria and abdominal distension after a surgical repair of urethral injury. To relieve the urethral stricture, we placed a self-expanding aortic stent graft extension with a partial coverage with an expanded polytetrafluoroethylene (ePTFE). After the placement of the stent, the dog presented with a normal urinary voiding, despite the presence of urinary incontinence. The current case indicates that the ePTFE-covered, self-expanding ASGE is an effective intervention for the treatment of severe urethral stricture in the dog.

著者

Mito Takayuki Kikkawa Yoshiaki Shimizu Akinori Hashizume Osamu Katada Shun Imanishi Hirotake Ota Azusa Kato Yukina Nakada Kazuto Hayashi Jun-Ichi

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.2, pp.e55789, 2013-02

被引用文献数

26 5

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Mitochondrial DNA (mtDNA) mutator mice are proposed to express premature aging phenotypes including kyphosis and hair loss (alopecia) due to their carrying a nuclear-encoded mtDNA polymerase with a defective proofreading function, which causes accelerated accumulation of random mutations in mtDNA, resulting in expression of respiration defects. On the contrary, transmitochondrial mito-miceΔ carrying mtDNA with a large-scale deletion mutation (ΔmtDNA) also express respiration defects, but not express premature aging phenotypes. Here, we resolved this discrepancy by generating mtDNA mutator mice sharing the same C57BL/6J (B6J) nuclear background with that of mito-miceΔ. Expression patterns of premature aging phenotypes are very close, when we compared between homozygous mtDNA mutator mice carrying a B6J nuclear background and selected mito-miceΔ only carrying predominant amounts of ΔmtDNA, in their expression of significant respiration defects, kyphosis, and a short lifespan, but not the alopecia. Therefore, the apparent discrepancy in the presence and absence of premature aging phenotypes in mtDNA mutator mice and mito-miceΔ, respectively, is partly the result of differences in the nuclear background of mtDNA mutator mice and of the broad range of ΔmtDNA proportions of mito-miceΔ used in previous studies. We also provided direct evidence that mtDNA abnormalities in homozygous mtDNA mutator mice are responsible for respiration defects by demonstrating the co-transfer of mtDNA and respiration defects from mtDNA mutator mice into mtDNA-less (ρ0) mouse cells. Moreover, heterozygous mtDNA mutator mice had a normal lifespan, but frequently developed B-cell lymphoma, suggesting that the mtDNA abnormalities in heterozygous mutator mice are not sufficient to induce a short lifespan and aging phenotypes, but are able to contribute to the B-cell lymphoma development during their prolonged lifespan.

著者

Kazuki Hotta Kentaro Kamiya Ryosuke Shimizu Misako Yokoyama Misao Nakamura-Ogura Minoru Tabata Daisuke Kamekawa Ayako Akiyama Michitaka Kato Chiharu Noda Atsuhiko Matsunaga Takashi Masuda

出版者

International Heart Journal Association

雑誌

International Heart Journal (ISSN:13492365)

巻号頁・発行日

vol.54, no.2, pp.59-63, 2013 (Released:2013-04-03)

参考文献数

31

被引用文献数

13 34

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The purpose of this study was to clarify the acute effects of a single session of stretching exercises on vascular endothelial function and peripheral circulation in patients with acute myocardial infarction. This study evaluated 32 patients (mean age, 66 ± 9 years) who received phase I cardiac rehabilitation after acute myocardial infarction. Five types of stretching exercises were performed on the floor: wrist dorsiflexion, close-legged trunk flexion, open-legged trunk flexion, open-legged lateral trunk bending, and cross-legged trunk flexion. Each exercise entailed a 30-second stretching followed by a 30-second relaxation, and was repeated twice. Low- and high-frequency components (LF and HF) of heart rate variability (LF, 0.04-0.15 Hz; HF, 0.15-0.40 Hz) were analyzed, and HF and LF/HF were used as indices of parasympathetic and sympathetic nervous activities, respectively. Reactive hyperemia peripheral arterial tonometry (RH-PAT) index was measured and used as a parameter for vascular endothelial function. Transcutaneous oxygen pressure (tcPO2) on the right foot and chest was also measured, and the Foot-tcPO2/Chest-tcPO2 ratio was used as a parameter for peripheral circulation. The HF, RH-PAT index, and Foot-tcPO2/Chest-tcPO2 ratio were significantly higher after the exercises than before (P < 0.05, P < 0.01, and P < 0.05, respectively). There was no significant difference in the LF/HF ratio measured before and after stretching exercises. These findings demonstrate that stretching exercises improve vascular endothelial function and peripheral circulation in patients with acute myocardial infarction.

著者

Kitahara Ken-ichi

出版者

Tukuba English Linguistic Society

雑誌

Tsukuba English Studies (ISSN:09116184)

巻号頁・発行日

vol.30, pp.23-50, 2011-10-01

著者

Megan G. Marks Jiandong Shi Michael O. Fry Zili Xiao Michelle Trzyna Vedavalli Pokala Michael A. Ihnat Pui-Kai Li

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.25, no.5, pp.597-604, 2002 (Released:2002-05-10)

参考文献数

72

被引用文献数

42 52 68

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Angiogenesis, in particular anti-angiogenesis, is an area of particular therapeutic interest in cancer treatment. Several anti-angiogenic agents are in the final stages of clinical trials. One of these agents, thalidomide, best known for its teratogenic potential, is showing promise against several tumor types. Thalidomide has been shown previously to require bio-activation to exert its anti-angiogenic effect in isolated blood vessels and endothelial cells. In this work, we confirmed these findings using the in utero chicken embryo chorioallantoic membrane (CAM) system. In particular, the anti-angiogenic effect of thalidomide is significantly enhanced by activation by human but not by rat liver microsomes. We also showed in the CAM assay that hydroxylation of thalidomide at either the 1′- or 5-position retained anti-angiogenic activity whereas its hydroxylation at the 4-position led to an inactive compound. We further demonstrated that thalidomide shows weak anti-proliferative activity against MDA-MB-231 human breast cancer cells in culture. Thalidomide showed slightly more anti-proliferative activity, however, against the SH-SY5Y human neuroblastoma and human umbilical vein endothelial cell (HUVEC) types. Furthermore, incubation of thalidomide with human liver microsomes added no additional anti-proliferative effect in these cell types versus thalidomide given alone. Finally, we report that none of the thalidomide metabolites tested had any anti-proliferative effect against the breast or neuroblastoma cells, but do possess appreciable anti-proliferative activity against the endothelial cells. In summary, this work suggests that hydroxylated thalidomide analogs based on putative metabolites of the drug possess significant anti-angiogenic activity and that exploring further derivatives of these as potential anti-angiogenic agents warrants further merit.

著者

Shujun Zhou Junzheng Wang Ayae Sugawara-Narutaki Atsushi Shimojima Tatsuya Okubo

出版者

The Chemical Society of Japan

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

vol.42, no.5, pp.481-482, 2013-05-05 (Released:2013-05-05)

参考文献数

10

被引用文献数

4

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Silica nanospheres (SNSs) synthesized with different base catalysts assemble into one-dimensional (1D) chain-like nanostructures at proper pH levels in the presence of the block copolymer F127. The addition of basic amino acids or the removal of base catalysts by dialysis influences the pH of 1D assembly. The base molecules affect assembly by modifying the electrostatic repulsion among the SNSs; however, they are not indispensable for this 1D assembly phenomenon.

著者

Toyotaka SATO Shin-ichi YOKOTA Torahiko OKUBO Kanako ISHIHARA Hiroshi UENO Yasukazu MURAMATSU Nobuhiro FUJII Yutaka TAMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.4, pp.407-414, 2013 (Released:2013-05-02)

参考文献数

38

被引用文献数

17 30

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Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samples and canine fecal samples. E. coli strains with high levels of fluoroquinolone resistance have been found to be frequently resistant to cephalosporins. Strains with high-level fluoroquinolone resistance exhibited lower intracellular enrofloxacin (ENR) concentrations, higher expression of AcrA, and a greater reduction in the fluoroquinolone minimum inhibitory concentration for treatment with an efflux pump inhibitor. The frequency of strains with enhanced ENR resistance selection and the survival rate of E. coli in the presence of ENR in vitro were correlated well with AcrA protein expression levels in the parental strains. These results suggest that AcrAB-TolC efflux pump over-expression is related to high-level fluoroquinolone resistance and the selection of strains with enhanced fluoroquinolone resistance.

著者

Seishiro CHIKAZAWA Yasutomo HORI Fumio HOSHI Kazutaka KANAI Naoyuki ITO Jun SATO Koichi ORINO Kiyotaka WATANABE Sei-ichi HIGUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.4, pp.515-517, 2013 (Released:2013-05-02)

参考文献数

15

被引用文献数

5 7

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We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.