著者

杉山 文博 高橋 智 水野 聖哉 Channabasavaiah B. Gurumurthy Aidan R. O'Brien Rolen M. Quadros John Adams Pilar Alcaide Shinya Ayabe Johnathan Ballard Surinder K. Batra Marie-Claude Beauchamp Kathleen A. Becker Guillaume Bernas David Brough Francisco Carrillo-Salinas Wesley Chan Hanying Chen Ruby Dawson Victoria DeMambro Jinke D'Hont Katharine M. Dibb James D. Eudy Lin Gan Jing Gao Amy Gonzales Anyonya R. Guntur Huiping Guo Donald W. Harms Anne Harrington Kathryn E. Hentges Neil Humphreys Shiho Imai Hideshi Ishii Mizuho Iwama Eric Jonasch Michelle Karolak Bernard Keavney Nay-Chi Khin Masamitsu Konno Yuko Kotani Yayoi Kunihiro Imayavaramban Lakshmanan Catherine Larochelle Catherine B. Lawrence Lin Li Volkhard Lindner Xian-De Liu Gloria Lopez-Castejon Andrew Loudon Jenna Lowe Loydie A. Jerome-Majewska Taiji Matsusaka Hiromi Miura Yoshiki Miyasaka Benjamin Morpurgo Katherine Motyl Yo-ichi Nabeshima Koji Nakade Toshiaki Nakashiba Kenichi Nakashima Yuichi Obata Sanae Ogiwara Mariette Ouellet Leif Oxburgh Sandra Piltz Ilka Pinz Moorthy P. Ponnusamy David Ray Ronald J. Redder Clifford J. Rosen Nikki Ross Mark T. Ruhe Larisa Ryzhova Ane M. Salvador Sabrina Shameen Alam Radislav Sedlacek Karan Sharma Chad Smith Katrien Staes Lora Starrs Fumihiro SUGIYAMA Satoru TAKAHASHI Tomohiro Tanaka Andrew W. Trafford Yoshihiro Uno Leen Vanhoutte Frederique Vanrockeghem Brandon J. Willis Christian S. Wright Yuko Yamauchi Xin Yi Kazuto Yoshimi Xuesong Zhang Yu Zhang Masato Ohtsuka Satyabrata Das Daniel J. Garry Tino Hochepied Paul Thomas Jan Parker-Thornburg Antony D. Adamson Atsushi Yoshiki Jean-Francois Schmouth Andrei Golovko William R. Thompson K. C. Kent Lloyd Joshua A. Wood Mitra Cowan Tomoji Mashimo Seiya MIZUNO Hao Zhu Petr Kasparek Lucy Liaw Joseph M. Miano Gaetan Burgio

出版者

BMC

雑誌

Genome Biology (ISSN:1474760X)

巻号頁・発行日

vol.20, no.1, 2019-08

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BackgroundCRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotides as donors (referred herein as "two-donor floxing" method).ResultsWe re-evaluate the two-donor method from a consortium of 20 laboratories across the world. The dataset constitutes 56 genetic loci, 17,887 zygotes, and 1718 live-born mice, of which only 15 (0.87%) mice contain cKO alleles. We subject the dataset to statistical analyses and a machine learning algorithm, which reveals that none of the factors analyzed was predictive for the success of this method. We test some of the newer methods that use one-donor DNA on 18 loci for which the two-donor approach failed to produce cKO alleles. We find that the one-donor methods are 10- to 20-fold more efficient than the two-donor approach.ConclusionWe propose that the two-donor method lacks efficiency because it relies on two simultaneous recombination events in cis, an outcome that is dwarfed by pervasive accompanying undesired editing events. The methods that use one-donor DNA are fairly efficient as they rely on only one recombination event, and the probability of correct insertion of the donor cassette without unanticipated mutational events is much higher. Therefore, one-donor methods offer higher efficiencies for the routine generation of cKO animal models.

著者

早川 陽 Yo Hayakawa

出版者

光葉会

雑誌

学苑 (ISSN:13480103)

巻号頁・発行日

no.896, pp.2-18, 2015-06

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In the field of Japanese art education, the pigments employed in traditional Japanese paintings are rarely used, though using them in an educational context could be very valuable in imparting an understanding of Bijutsu Bunka(art culture)currently required in curriculum guidelines. In an attempt to explore how they can be adopted in today's art education, this paper explores two Japanese traditional painting technique books from the Taisho Period and highlights the common characteristics of the pigments described in the books and compares them with pigments that have survived from those times, or have been more recently developed for use in traditional-style paintings. The origins of various Japanese pigments are organized in such a way that they can be used as educational material. In order to provide background for this research, the first chapter considers how Japanese traditional paintings are created, displayed and enjoyed today. Also the significance of Bijutsu Bunka, which was newly specified in curriculum guidelines, is discussed. The second chapter, focusing on the refinement and elutriation of pigments used in Japanese traditional paintings, categorizes and organizes the features of the pigments used. The third chapter refers to the above two books and considers the changes made since then in the types of the pigment. The final chapter summarizes the characteristics of the pigments and gives a general view of how they were traditionally used and concludes that the pigments, many of which have been refined by elutriation, can be utilized effectively in the field of art education today. The author believes that intercourse between the past and present, and understanding and appreciating traditional art, offer new possibilities in the future of art education.

著者

秋岡 陽 Yo Akioka

出版者

フェリス女学院大学

雑誌

フェリス女学院大学キリスト教研究所紀要 (ISSN:24239127)

巻号頁・発行日

vol.02, pp.71-99, 2017-03

著者

Yutaka WADA Yo KAWABATA Kenji OGIMOTO Hiroaki AKIYAMA Takao YANAGI Seiichi SAKAMOTO

出版者

THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES

雑誌

TRANSACTIONS OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES, AEROSPACE TECHNOLOGY JAPAN (ISSN:18840485)

巻号頁・発行日

vol.16, no.6, pp.506-510, 2018 (Released:2018-09-04)

参考文献数

11

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In this study, a reusable educational motor that can burn different types of materials in the chamber was developed. Specifically, five candidate sweets were selected as fuel and burnt. The combustion performances of these sweets were compared, and the soft candy was selected as the solid fuel to be used in the motor, because it had the highest total impulse. The SOUKI Systems Co. Ltd. designed a small candy hybrid rocket based on these results. The candy rocket was launched successfully using soft candy at the Kada Cosmo Park. The motor carried the candy rocket to 319 m altitude and was fully functional under the high-acceleration environment.

著者

Junro KATO Tatsuya ODATE Yo-Han KIM Toshihiro ICHIJO Shigeru SATO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.81, no.7, pp.958-967, 2019 (Released:2019-07-11)

参考文献数

49

被引用文献数

1

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The objective of the present study was to identify the effect of feeding management on disease incidence and blood metabolite levels in dairy herds in Iwate Prefecture, Japan. A generalized linear model approach was used to identify the risk factors for ketosis and displaced abomasum (DA) in dairy herds (n=30), and metabolic profile test (MPT) results were compared to verify the involvement of the factors. Consequently, the proportion of corn silage (CS) with ≥30% of dry matter (DM) fed to cows during the lactation period was confirmed as the most reliable risk factor for ketosis, while no risk factor was identified for DA. Meanwhile, the incidence rates of ketosis and DA were significantly (P<0.05) higher in the herds that were fed CS (n=20) than in those fed a non-CS diet (n=10). When the MPT results of the herds fed with CS containing ≥30% of DM (HCS group, n=4; 76 cows), with CS containing <30% of DM (LCS group, n=14; 285 cows), and a non-CS diet (NCS group, n=12; 236 cows) were compared, the HCS group showed higher beta-hydroxybutyric and lower blood urea nitrogen concentrations for until 49 days after parturition. Overall, feeding cows with CS diets containing over 30% of DM might increase their risk of developing negative energy and protein balances, thereby resulting in increasing incidences of ketosis in the Iwate Prefecture.

著者

Tomonari Akiko Katanuma Akio Matsumori Tomoaki Yamazaki Hajime Sano Itsuki Minami Ryuki Sen-yo Manabu Ikarashi Satoshi Kin Toshifumi Yane Kei Takahashi Kuniyuki Shinohara Toshiya Maguchi Hiroyuki

出版者

WJG Press

雑誌

World Journal of Gastroenterology (ISSN:22192840)

巻号頁・発行日

vol.21, no.27, pp.8458-8461, 2015-07-21

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Endoscopic ultrasonography-guided fine needle aspiration (EUS-FNA) is a useful and relatively safe tool for the diagnosis and staging of pancreatic cancer. However, there have recently been several reports of tumor seeding after EUS-FNA of adenocarcinomas. A 78-year-old man was admitted to our hospital due to upper gastric pain. Examinations revealed a 20 mm mass in the pancreatic body, for which EUS-FNA was performed. The cytology of the lesion was adenocarcinoma, and the stage of the cancer was T3N0M0. The patient underwent surgery with curative intent, followed by adjuvant chemotherapy with S-1. An enlarging gastric submucosal tumor was found on gastroscopy at 28 mo after surgery accompanied by a rising level of CA19-9. Biopsy result was adenocarcinoma, consistent with a pancreatic primary tumor. Tumor seeding after EUS-FNA was strongly suspected. The patient underwent surgical resection of the gastric tumor with curative intent. The pathological result of the resected gastric specimen was adenocarcinoma with a perfectly matched mucin special stain result with the previously resected pancreatic cancer. This is the first case report of tumor seeding after EUS-FNA which was surgically resected and inspected pathologically.

著者

Seung Kyu Park Dae Jung Yang Je Ho Kim Jae Won Heo Sam Heon Park Yo Han Uhm Jong Hyuk Yoon

出版者

The Society of Physical Therapy Science

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.31, no.1, pp.24-28, 2019 (Released:2019-01-10)

参考文献数

17

被引用文献数

2

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[Purpose] To present an efficient treatment regimen for patients with cervicogenic headache by comparatively analyzing the neck disability index (NDI) and cervical muscle activity after an exercise intervention. [Participants and Methods] Thirty patients with cervicogenic headache were assigned to the cranio-cervical flexion group (n=15) and cranio-cervical flexion plus transcranial direct current stimulation (tDCS) group (n=15). Intervention was administered for four weeks, after which the participants’ NDI and sternocleidomastoid muscle activity were measured. [Results] The treatment group demonstrated a significantly greater change in NDI after the intervention compared to the control group. The treatment group also showed a significantly greater change in sternocleidomastoid muscle activity than the control group. [Conclusion] Our results show that applying tDCS during cranio-cervical flexion exercise can strengthen the sternocleidomastoid muscle more effectively while improving pain and associated functions in patients with cervicogenic headache. These results would contribute towards developing a more efficient treatment for patients with cervicogenic headache.

著者

Hono Takahiro Kishida Mayumi Misao Junko Shimada Osamu Miyata Yo

出版者

川崎医療福祉大学

雑誌

Kawasaki journal of medical welfare (ISSN:13415077)

巻号頁・発行日

vol.2, no.1, pp.69-72, 1996

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The present study examined the relationships between Type A/B behaviors and sleep habits, and morningness-eveningness types. Undergraduate students (35 males, 125 females) responded to three questionnaires : the KG's (Kwansei Gakuin University) Daily Life Questionnaire, the Japanese version of Morningness-Eveningness Questionnaire (ME) and the Sleep Habits Questionnaire. The results showed that there was no significant correlation between Type A score and sleep habits (sleep duration, wake-up time and bedtime), and that there was also no significant correlation between Type A score and ME score. However, two weak correlations were found between two subscales and sleep habits. HT (hard driving-time urgency) score was negatively correlated with the habitual sleep duration, and SP (speed-power) score was positively correlated with wake-up time. Thus it might be that substructures of Type A behavior are associated with sleep habits.

著者

Akira MATSUI Tomoko OSAWA Hirofumi FUJIKAWA Yo ASAI Tohru MATSUI Hideo YANO

出版者

Japanese Society of Equine Science

雑誌

Journal of Equine Science (ISSN:13403516)

巻号頁・発行日

vol.13, no.4, pp.109-112, 2002 (Released:2003-04-10)

参考文献数

13

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We examined total sweating rate (SR) and the amount of mineral (Na, K, Ca, Mg, Fe, P, Zn and Cu) loss through sweat in 2-year-old horses during exercise for approximately 2,000 m at a speed of 700 m/min, at cool ambient temperature. The total SR was estimated by the unit area SR on the neck by filter paper within a capsule. Mean total SR was 1.55 ± 0.47 (SD) kg. The ratio of sodium loss to the requirements reported by National Research Council reached 23% and that of potassium was 7%. The ratio of other mineral losses to the requirements reported by National Research Council was 2% or less. These results suggested that sodium had to be supplemented to the horse, but there was no need to add extra minerals to the diet to compensate for mineral losses of calcium, magnesium, iron, phosphorus, zinc and copper through sweat during exercise with light intensity and at a cool ambient temperature.

著者

Yo-Soon Bang Kyung Hyun Son Hyun Jin Kim

出版者

The Society of Physical Therapy Science

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.28, no.11, pp.3112-3115, 2016 (Released:2016-11-29)

参考文献数

21

被引用文献数

2 24

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[Purpose] The purpose of this study is to investigate the effects of virtual reality training using Nintendo Wii on balance and walking for stroke patients. [Subjects and Methods] Forty stroke patients with stroke were randomly divided into two exercise program groups: virtual reality training (n=20) and treadmill (n=20). The subjects underwent their 40-minute exercise program three times a week for eight weeks. Their balance and walking were measured before and after the complete program. We measured the left/right weight-bearing and the anterior/posterior weight-bearing for balance, as well as stance phase, swing phase, and cadence for walking. [Results] For balance, both groups showed significant differences in the left/right and anterior/posterior weight-bearing, with significant post-program differences between the groups. For walking, there were significant differences in the stance phase, swing phase, and cadence of the virtual reality training group. [Conclusion] The results of this study suggest that virtual reality training providing visual feedback may enable stroke patients to directly adjust their incorrect weight center and shift visually. Virtual reality training may be appropriate for patients who need improved balance and walking ability by inducing their interest for them to perform planned exercises on a consistent basis.

著者

Seung Kyu Park Dae Jung Yang Yo Han Uhm Jae Won Heo Je Ho Kim

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.28, no.7, pp.2055-2058, 2016 (Released:2016-07-29)

参考文献数

23

被引用文献数

6

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[Purpose] The purpose of this study was to examine the effect of virtual reality-based eccentric training on lower extremity muscle activity and balance in stroke patients. [Subjects and Methods] Thirty stroke patients participated, with 15 patients allotted to each of two eccentric training groups: one using a slow velocity (group I) and one using a fast velocity (group II). The virtual reality-based eccentric training was performed by the patients for 30 minutes once a day, 5 days a week, for 8 weeks using an Eccentron system. Surface electromyography was used to measure the lower extremity muscle activity, while a BioRescue was used to measure balancing ability. [Results] A significant difference in lower extremity muscle activation and balance ability was observed in group I compared with group II. [Conclusion] This study showed that virtual reality-based eccentric training using a slow velocity is effective for improving lower extremity muscle activity and balance in stroke patients.

著者

Yo Kunii Takashi Uruno Koji Mukasa Kenichi Sekiya Kenji Iwaku Akifumi Suzuki Kiminori Sugino Jaeduk Yoshimura Noh Koichi Ito

出版者

(社)日本内分泌学会

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

vol.63, no.1, pp.21-27, 2016 (Released:2016-01-31)

参考文献数

20

被引用文献数

1 5

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In the event of a nuclear power plant accident, prophylactic administration of potassium iodide (KI) is recommended to prevent thyroid damage due to uptake of radioiodine. To assess the inhibitory effect of low-dose inorganic iodine on thyroidal radioactive iodine uptake (RAIU) in healthy adults without dietary iodine restriction, single or repeated doses of 10 mg inorganic iodine solution were given to 22 Japanese volunteers, 18 men and 4 women with the mean age of 35.7 years, between 2011 and 2013. Changes in urinary iodine excretion, thyroid function and 24-hour RAIU were also evaluated. The median 24-hour RAIU without iodine restriction was 13 % (range, 5-26 %). A single-dose of 10 mg inorganic iodine suppressed the median 24-hour RAIU measured one hour after iodine administration to 3 % (range, 1-7 %) and, in 90.9% of 22 participants their 24-hour RAIU was < 5 %. For seven participants given 10 mg of inorganic iodine daily for 14 days, the median 24-hour RAIU measured at 24 hours after the last administration of iodine was 6 % (range, 2-12 %), although the inhibitory effect was diminished in two participants. Serum thyroid stimulating hormone concentration was slightly elevated in three participants without decreased serum FT3 and FT4 levels. We conclude that a single-dose of 10 mg inorganic iodine is sufficient to inhibit RAIU in adults, although the inhibitory effect of repeated-dose on RAIU is diminished when KI is given once daily. The dose, duration or interval of iodine administration should be evaluated in iodine-sufficient regions in a future.

著者

大森 慈子 宮田 洋 Yasuko Omori Yo Miyata

雑誌

人文論究 (ISSN:02866773)

巻号頁・発行日

vol.47, no.4, pp.67-78, 1998-02-20

著者

余 健 YO Ken

出版者

三重大学教育学部

雑誌

三重大学教育学部研究紀要, 自然科学・人文科学・社会科学・教育科学 (ISSN:18802419)

巻号頁・発行日

vol.66, pp.217-225, 2015-03-31

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2009年度以降、三重県四日市市南部地域で継続してきた臨地方言調査の調査結果を生かした三重大生による各小学校での授業の内、2011年2月に実施した水沢小学校での授業に焦点を当て、その成果と課題の検証を行った。三重のことばに自信を持てていない若年者が多い中、公教育の場で、ビデオ教採等を有効に活用しながら、方言を題材に取り上げる意義は大きいといえる。

著者

Sato Yo Hong Hea Nam Yanai Nobuaki OBINATA Masuo

出版者

Japanese Biochemical Society

雑誌

The journal of biochemistry (ISSN:0021924X)

巻号頁・発行日

vol.124, no.1, pp.209-216, 1998-07-01

参考文献数

20

被引用文献数

4

著者

Lina WAKAKO Yo-ichi MATSUMOTO Hiroyuki KANAI Toyonori NISHIMATSU Hirokazu KIMURA Hideo MOROOKA

出版者

一般社団法人 日本繊維機械学会

雑誌

Journal of Textile Engineering (ISSN:13468235)

巻号頁・発行日

vol.54, no.2, pp.33-39, 2008-04-15 (Released:2008-07-08)

参考文献数

19

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To examine the aesthetic properties of pantyhose, an image analysis and wear experiment were carried out. We analyzed both the area ratio of light, medium, and dark lightness areas and the position of three areas in the distribution of lightness difference between legs with and without pantyhose fabric. The data was used to determine what effect these two variables have on the visual appeal of legs in pantyhose. Moreover, we examined the relationship between pantyhose size and women’s leg size on the attractiveness of legs in pantyhose made from single covered yarn with colored polyurethane core yarn. The results showed that, for the enhanced visual appeal of legs in pantyhose, (1) the area ratio and position of three areas in the distribution of lightness difference were very important factors; (2) it was desirable to design and produce the appropriate size of pantyhose for various sized legs in order to enhance their attractiveness.

著者

三好 真琴 鴛海 良彦 鴨井 逸馬 一矢 有一 西谷 弘 松浦 啓一 城 邦男 小野 庸 ミヨシ マコト オシウミ ヨシヒコ カモイ イツマ イチヤ ユウイチ ニシタニ ヒロム マツウラ ケイイチ ジョウ クニオ オノ ヨウ Miyoshi Makoto Oshiumi Yoshihiko Kamoi Itsuma Ichiya Yuichi Nishitani Hiromu Matsuura Keiichi Jo Kunio Ono Yo

出版者

日本医学放射線学会

雑誌

日本医学放射線学会雑誌 (ISSN:00480428)

巻号頁・発行日

vol.40, no.5, pp.458-465, 1980-05-25

著者

Atsumi Shinji Takai Noriyasu Yoshida Yo

出版者

歯科基礎医学会

雑誌

歯科基礎医学会雑誌 (ISSN:03850137)

巻号頁・発行日

vol.40, no.4, pp.213-219, 1998-08-20

著者

YO

出版者

社団法人情報科学技術協会

雑誌

ドクメンテーション研究 (ISSN:00125180)

巻号頁・発行日

vol.17, no.3, 1967-06