著者
Nakano Yuriko Tsukagoshi Akira
出版者
日本動物分類学会
雑誌
Species diversity : an international journal for taxonomy, systematics, speciation, biogeography, and life history research of animals (ISSN:13421670)
巻号頁・発行日
vol.7, no.1, pp.67-115, 2002-02-28
被引用文献数
8

The brackish-water ostracod fauna was surveyed for almost two years at 17 points representing typical environments, such as foreshore sand flat, back marsh, and tidal creek, in the intertidal zone of the Obitsu River Estuary, Chiba Prefecture, central Japan. Twenty-one species belonging to 17 genera and 10 families were identified. In addition to the description of one new genus, Palusleptocythere, and four new species, Pontocythere xiphoidea, Palusleptocythere migrans, Loxoconcha kosugii, and Cytherois ikeyai, chitinous parts are described for the first time for four previously described species, Spinileberis pulchra Chen, 1982, Angulicytherura ? miii (Ishizaki, 1969), Semicytherura wakamurasaki Yajima, 1982, and Loxoconcha pulchra Ishizaki, 1968. Furthermore chitinous parts of male Dolerocypria mukaishimensis Okubo, 1980 are also shown for the first time. This is the first report on an ostracod fauna from a shallow estuarine environment in either Japan or any adjacent area.
著者
Ryota NOMURA Mitsuyuki SHIRAI Yukio KATO Masaru MURAKAMI Kazuhiko NAKANO Norihiko HIRAI Tetsuya MIZUSAWA Shuhei NAKA Yoshie YAMASAKI Michiyo MATSUMOTO-NAKANO Takashi OOSHIMA Fumitoshi ASAI
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.1202220780, (Released:2012-03-02)
被引用文献数
8 16

Porphyromonas gulae, a gram-negative black-pigmented anaerobe, is a pathogen for periodontitis in dogs. An approximately 41-kDa fimbrial subunit protein (FimA) encoded by fimA is regarded as associated with periodontitis. In the present study, the fimA genes of 17 P. gulae strains were sequenced, and classified into two major types. The generation of phylogenetic trees based on the deduced amino acid sequence of FimA of P. gulae strains along with sequences from several strains of Porphyromonas gingivalis, a major cause of human periodontitis, revealed that the two types of FimA (types A and B) of P. gulae were similar to type I FimA and types II and III FimA of P. gingivalis, respectively. A PCR system for classification was established based on differences in the nucleotide sequences of the fimA genes. Analysis of 115 P. gulae-positive oral swab specimens from dogs revealed that 42.6, 22.6, and 26.1% of them contained type A, type B, and both type A and B fimA genes, respectively. Experiments with a mouse abscess model demonstrated that the strains with type B fimA caused significantly greater systemic inflammation than those with type A. These results suggest that the FimA proteins of P. gulae are diverse with two major types and that strains with type B fimA could be more virulent.
著者
Kazuya EDAMURA Takashi KUROSAWA Rei NAKANO Kenji TESHIMA Kazushi ASANO Shigeo TANAKA
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.1201210761, (Released:2012-01-27)
被引用文献数
2 2

We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5%, 10%, and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.
著者
奥島 実 SUGINO Dan KOUNO Yoshio NAKANO Shigeru MIYAHARA Junichi TODA Hisashi KUBO Shigemasa MATSUSHIRO Aizo
出版者
Genetics Society of Japan
雑誌
遺伝学雑誌 (ISSN:0021504X)
巻号頁・発行日
vol.66, no.2, pp.173-187, 1991
被引用文献数
8 18

A bacterial strain, which assimilated dextran and water-insoluble glucan produced by <i>Streptococcus mutans</i>, was isolated from soil. The bacterium produced and secreted potent dextranase activity, which was identified as <i>Arthrobacter sp</i>. and named CB-8. The dextranase was purified and some enzymatic properties were characterized. The enzyme efficiently decomposed the water-insoluble glucan as well as dextran. A gene library from the bacteria was constructed with <i>Escherichia coli</i>, using plasmid pUC19, and clones producing dextranase activity were selected. Based on the result of nucleotide sequencing analysis, it was deduced that the dextranase was synthesized in CB-8 cells as a polypeptide precursor consisting of 640 amino acid residues, including 49 N-terminal amino acid residues which could be regarded as a signal peptide. In the <i>E. coli</i> transformant, the dextranase activity was detected mostly in the periplasmic space. The gene for the dextranase was introduced into <i>Streptococcus sanguis</i>, using an <i>E. coli-S</i>. <i>sanguis</i> shuttle vector that contained the promoter sequence of a gene for glucosyltransferase derived from a strain of <i>S. mutans</i>. The active dextranase was also expressed and accumulated in <i>S. sanguis</i> cells.<br>
著者
NAKANO Takumi KOMATSUDAIRA Yoshiki SHIOMI Akichika IMAI Masaharu
出版者
一般社団法人電子情報通信学会
雑誌
IEICE transactions on fundamentals of electronics, communications and computer sciences (ISSN:09168508)
巻号頁・発行日
vol.82, no.11, pp.2375-2382, 1999-11-25
被引用文献数
3

In a real-time system, it is required to reduce the response time to an interrupt signal, as well as the execution time of a Real-Time Operating System (RTOS). In order to satisfy this requirement, we have proposed a method of implementing some of the functionalities of an RTOS using hardware. Based on this idea, we have implemented a VLSI chip, called STRON (silicon TRON: The Realtime Operating system Nucleus), to enhance the performance of an RTOS, where the STRON chip works as a peripheral unit of any MPU. In this paper we describe the hardware architecture of the STRON chip and the performance evaluation results of the RTOS using the STRON chip. The following results were obtained. (1) The STRON chip is implemented in only about 10,000 gates when the number of each object (task, event flag, semaphore, and interrupt) is 7. (2) The task scheduler can execute within 8 clocks in a fixed period using the hardware algorithm when the number of tasks is 7. (3) Most of the basic μITRON system calls using the STRON chip can be executed in a fixed period of a few microseconds. (4) The execution time of a system call, measured by a multitask application program model, can be reduced to about one-fifth that in the case of the conventional software RTOS. (5) The total performance, including context switching, is about 2.2 times faster than that of the software RTOS. We conclude that the execution time of the part of the system call implemented by the STRON chip can almost be ignored, but the part of the interface software and context switching related to the architecture of a MPU strongly influence the total performance of an RTOS.
著者
Nakano Hirokazu Yoshida Akihide Ogasawara Kazushi Sanjo Akira Tanaka Shigeru Kamegai Takuya Satoh Kazuro Miura Hiroyuki
出版者
日本矯正歯科学会
雑誌
Orthodontic waves : journal of the Japanese Orthodontic Society : 日本矯正歯科学会雑誌 (ISSN:13440241)
巻号頁・発行日
vol.60, no.6, pp.398-401, 2001

The purpose of this study was to clarify the surface roughness of 31 brands of titanium alloy orthodontic wires from 13 manufacturers using a confocal optical microscope. Cobalt-chrome and stainless steel wire were also examined as a reference of comparison. The following results were obtained ; (1) Mean Ra, as determined from the lengthway axis of titanium alloy wires, was 0.296μm, and that determined from the widthway axis was 0.440μm. The modulus of Ra was 0.368μm. (2) For titanium alloy orthodontic wires, the greatest amount of modulus of Ra was 1.244μm and the lowest was 0.135μm. (3) Modulus of Ra was 0.140μm for the cobalt-chrome wire and 0.154μm for the stainless steel wire, each lower tendency than titanium alloy. As a result of our findings, we consider it necessary to select the smoother wire possible when using a sliding mechanism, if the mechanical properties of available wires are nearly the same.