著者

中野 春夫 Haruo Nakano

出版者

學習院大學文學部

雑誌

研究年報 = The annual collection of essays and studies, Faculty of Letters (ISSN:04331117)

巻号頁・発行日

no.68, pp.25-48, 2022-03-20

著者

Kentaro Mori Yu Iida Akira Tamase Motohiro Nomura Yoshihisa Kitamura Yuichi Kawabata Tatsu Nakano

出版者

The Japanese Society for Neuroendovascular Therapy

雑誌

Journal of Neuroendovascular Therapy (ISSN:18824072)

巻号頁・発行日

pp.ai.2017-0087, (Released:2018-01-18)

参考文献数

3

被引用文献数

1 1

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This study describes hyperintensity of dural venous sinuses due to jugular venous reflux (JVR) and their respiration- and position-induced changes, and discusses the significance of this phenomenon in endovascular treatment.

著者

中野 幡能 H. Nakano

雑誌

研究紀要

巻号頁・発行日

vol.9, pp.1-5, 1972-03-31

著者

Tomoyuki Sasaki Hidehiro Nakano

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

Nonlinear Theory and Its Applications, IEICE (ISSN:21854106)

巻号頁・発行日

vol.14, no.2, pp.267-291, 2023 (Released:2023-04-01)

参考文献数

26

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This paper relates to study on a new deterministic particle swarm optimization (D-PSO) called Optimizer based on Spiking Neural-oscillator Networks (OSNNs). OSNNs have a swarm consisting of plural particles which search a solution space interacting with each other. A single particle consists of plural spiking neural oscillators (`spiking oscillators') modeled by integrate-and-fire neurons. The spiking oscillators are coupled by a network topology and interact with each other by exchanging their own spike signals. Such interaction results in that coupling spiking oscillators can take synchronous or asynchronous dynamics and affects search performances of OSNNs. Herein we propose the basic algorithm of OSNNs and applied Ring 1-way network topology to coupling spiking oscillators. We theoretically analyzed parameter conditions for OSNNs, demonstrated the analytic results, and verified search performances of OSNNs through numerical simulations. We also herein discuss search performances of OSNNs and the relationship between the search performances and analytic results, and clarify prospective parameter regions which lead to good search performances in solving optimization problems.

著者

中野 逸雄 ナカノ イツオ Nakano Itsuo

出版者

大阪大学大学院文学研究科

雑誌

待兼山論叢. 美学篇 (ISSN:03874818)

巻号頁・発行日

vol.45, pp.1-23, 2011-12-26

著者

Katsumi YAMAGUCHI Hiroshi NAKANO Masahiro MURAKAMI Shoji KONOSU Osamu NAKAYAMA Midori KANDA Akihiro NAKAMURA Hiroaki IWAMOTO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.51, no.2, pp.493-498, 1987 (Released:2006-04-05)

参考文献数

33

被引用文献数

28

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Botryococcus braunii strain Austin and strain Berkeley cultured under the same conditions contained 27.4g and 34.0g of lipids per 100g of dry alga, respectively. The lipids in the former strain were 25.5% hydrocarbons, 58.8% other nonpolar lipids, and 15.7% polar lipids, and those in the latter strain were 71.6%, 9.2%, and 19.2%, respectively. Distinct differences were also observed in the components of the nonpolar and polar lipids, especially in the latter, between the strains. The fatty acid composition of the nonpolar lipid fraction in Austin strain was unique, oleic acid being 81% of the total acids.

著者

Tomoko Seki Toru Taniuchi Hideki Nakano Makoto Shimizu

出版者

The Japanese Society of Fisheries Science

雑誌

Fisheries science (ISSN:09199268)

巻号頁・発行日

vol.64, no.1, pp.14-20, 1998 (Released:2008-06-30)

参考文献数

16

被引用文献数

21 24

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We studied age, growth, and maturity of the oceanic whitetip shark Carcharhinus longimanus in the North Pacific, and the reproduction in the North and South Pacific, captured with tuna longline from Nov. 1967 to Oct. 1995. Vertebral rings were examined from 111 males (precaudal length: 54-172cm) and 114 females (precaudal length: 50-195cm). Minimum and maximum numbers of translucent rings were 0 and 11. Marginal increment analysis suggested annualus deposition occurs during spring. A growth difference between sexes was not found. A von Bertalanfly growth equation combining both sexes was as follows; Lt=244.58×{1-e-0.103×(t+2.698)}Lt was expressed as precaudal length in cm at age t. The reproductive condition was examined from 136 males and 85 females. Maturation was determined to be when precaudal length was between 125 and 135cm for both sexes (4-5 years). The parturition period is extended over a long duration. The birth size was 45-55cm and litter size varied from 1-14 with a mean of approximately six in the North Pacific.

著者

Naruhiko Sunada Hiroyuki Honda Yasuhiro Nakano Koichiro Yamamoto Kazuki Tokumasu Yasue Sakurada Yui Matsuda Toru Hasegawa Yuki Otsuka Mikako Obika Yoshihisa Hanayama Hideharu Hagiya Keigo Ueda Hitomi Kataoka Fumio Otsuka

出版者

The Japan Endocrine Society

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

vol.69, no.10, pp.1173-1181, 2022 (Released:2022-10-28)

参考文献数

34

被引用文献数

2 26

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Symptoms of long COVID are complex and long-lasting, and endocrine dysfunction might be involved in the underlying mechanisms. In this study, to clarify the hormonal characteristics of long COVID patients, laboratory data for patients who visited the outpatient clinic for long COVID were evaluated. A retrospective analysis was performed for patients who visited Okayama University Hospital during the period from Feb 2021 to Dec 2021 with focus on the interrelationships between major symptoms and endocrine data. Information and laboratory data were obtained from medical records for 186 patients. The patients had various symptoms, and the most frequent symptoms were general malaise, dysosmia/dysgeusia, hair loss, headache, dyspnea, and sleeplessness. Patients who were suffering from fatigue and dysosmia/dysgeusia were younger, while hair loss was more frequent in older and female patients. As for the characteristics of patients suffering from general fatigue, the scores of depression and fatigue were positively correlated with serum levels of cortisol and free thyroxin (FT4), respectively. Also, patients suffering from general fatigue had lower levels of serum growth hormone and higher levels of serum FT4, while patients with dysosmia/dysgeusia had a significantly lower level of serum cortisol. Serum thyrotropin (TSH) levels were higher and the ratios of FT4/TSH were lower in the initially severe cases, suggesting occult hypothyroidism. In addition, the ratios of plasma adrenocorticotropin to serum cortisol were decreased in patients with relatively high titers of serum SARS-CoV-2 antibody. Thus, hormonal changes seem to be, at least in part, involved in the persistent symptoms of long COVID.

著者

Takahiko Sakai Hefei Li Tatsuki Shimada Suzune Kita Maho Iida Chunwoo Lee Tamaki Nakano Satoshi Yamaguchi Satoshi Imazato

出版者

Japan Prosthodontic Society

雑誌

Journal of Prosthodontic Research (ISSN:18831958)

巻号頁・発行日

pp.JPR_D_22_00053, (Released:2022-08-25)

参考文献数

38

被引用文献数

14

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Purpose: This study aimed to develop an artificial intelligence (AI) model to support the determination of an appropriate implant drilling protocol using cone-beam computed tomography (CBCT) images.Methods: Anonymized CBCT images were obtained from 60 patients. For each case, after implant placement, images of the bone regions at the implant site were extracted from 20 slices of CBCT images. Based on the actual drilling protocol, the images were classified into three categories: protocols A, B, and C. A total of 1,200 images were divided into training and validation datasets (n = 960, 80%) and a test dataset (n = 240, 20%). Another 240 images (80 images for each type) were extracted from the 60 cases as test data. An AI model based on LeNet-5 was developed using these data sets. The accuracy, sensitivity, precision, F-value, area under the curve (AUC) value, and receiver operating curve were calculated.Results: The accuracy of the trained model is 93.8%. The sensitivity results for drilling protocols A, B, and C were 97.5%, 95.0%, and 85.0%, respectively, while those for protocols A, B, and C were 86.7%, 92.7%, and 100%, respectively, and the F values for protocols A, B, and C were 91.8%, 93.8%, and 91.9%, respectively. The AUC values for protocols A, B, and C are 98.6%, 98.6%, and 99.4%, respectively.Conclusion: The AI model established in this study was effective in predicting drilling protocols from CBCT images before surgery, suggesting the possibility of developing a decision-making support system to promote primary stability.

著者

Itaru Miura Masanori Nagao Hironori Nakano Kanako Okazaki Fumikazu Hayashi Mayumi Harigane Shuntaro Itagaki Hirooki Yabe Masaharu Maeda Tetsuya Ohira Tetsuo Ishikawa Seiji Yasumura Kenji Kamiya

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

vol.32, no.Supplement_XII, pp.S95-S103, 2022-12-05 (Released:2022-12-05)

参考文献数

34

被引用文献数

4

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Background: The relationship between radiation levels and mental health status after a nuclear disaster is unknown. We examined the association between individual external radiation doses and psychological distress or post-traumatic stress after the Fukushima Daiichi nuclear power plant accident in March 2011 in Japan.Methods: The Mental Health and Lifestyle Survey was conducted from January 2012. Based on the estimated external radiation doses for the first 4 months, a total of 64,184 subjects were classified into <1 mSv, 1 to <2 mSv, and ≥2 mSv groups. Odds ratios (ORs) and 95% confidence intervals (CIs) of psychological distress and post-traumatic stress, with the <1 mSv group as the reference, were calculated using logistic regression analysis adjusted for age, sex, evacuation, perception of radiation risk, and subjective health status.Results: The prevalence of psychological distress/post-traumatic stress in the <1 mSv, 1 to <2 mSv, and ≥2 mSv groups was 15.1%/22.1%, 14.0%/20.1%, and 15.0%/21.7%, respectively. In women, although the ≥2 mSv group tended to have a higher risk of psychological distress with the age-adjusted OR of 1.13 (95% CI, 0.99–1.30), the adjusted OR decreased to 1.00 (95% CI, 0.86–1.16) after controlling for all variables. On the other hand, there were no dose-dependent associations between radiation dose and post-traumatic stress.Conclusion: Although external radiation doses were not associated with psychological distress, evacuation and perception of radiation risk may increase the risk of psychological distress in women in the higher dose group.

著者

Akira Sakai Masanori Nagao Hironori Nakano Tetsuya Ohira Tetsuo Ishikawa Mitsuaki Hosoya Michio Shimabukuro Atsushi Takahashi Junichiro J. Kazama Kanako Okazaki Fumikazu Hayashi Seiji Yasumura Hitoshi Ohto Kenji Kamiya

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

vol.32, no.Supplement_XII, pp.S84-S94, 2022-12-05 (Released:2022-12-05)

参考文献数

42

被引用文献数

6

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Background: Associations have been reported between lifestyle-related diseases and evacuation after the Great East Japan Earthquake (GEJE). However, the relationship between lifestyle-related diseases and the effective radiation dose due to external exposure (EDEE) after the GEJE remains unclear.Methods: From among 72,869 residents of Fukushima Prefecture (31,982 men; 40,887 women) who underwent a comprehensive health check in fiscal year (FY) 2011, the data of 54,087 residents (22,599 men; 31,488 women) aged 16 to 84 years were analyzed. The EDEE data of 25,685 residents with incomplete results from the basic survey, performed to estimate the external radiation exposure dose, were supplemented using multiple imputation. The data were classified into three groups based on EDEE (0 to <1, 1 to <2, and ≥2 mSv groups and associations between the incidence of diseases and EDEE from FY2011 to FY2017 were examined using a Cox proportional hazards model, with FY2011 as the baseline.Results: A higher EDEE was associated with a greater incidence of hypertension, diabetes mellitus, dyslipidemia, hyperuricemia, liver dysfunction, and polycythemia from FY2011 to FY2017 in the age- and sex-adjusted model. However, after further adjustment for evacuation status and lifestyle-related factors, the significant associations disappeared. No association was found between EDEE and other lifestyle-related diseases.Conclusion: EDEE was not directly associated with the incidence of lifestyle-related diseases after the GEJE. However, residents with higher external radiation doses in Fukushima Prefecture might suffer from lifestyle-related diseases related to evacuation and the resultant lifestyle changes.

著者

Mayu Yasuda Uemura Tetsuya Ohira Seiji Yasumura Akira Sakai Atsushi Takahashi Mitsuaki Hosoya Masanori Nagao Hironori Nakano Hitoshi Ohto Kenji Kamiya

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

vol.32, no.11, pp.496-501, 2022-11-05 (Released:2022-11-05)

参考文献数

27

被引用文献数

2

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Background: The proportion of overweight individuals living in the evacuation zone of Fukushima increased after the Great East Japan Earthquake. However, the change in the prevalence of abdominal obesity has not been reported. Lifestyle habits and changes in these habits after the disaster might have affected the onset of abdominal obesity; however, the association between the two is unclear.Methods: This study evaluated 19,673 Japanese participants of the Fukushima Health Management Survey. We used data from general health check-ups conducted in 13 municipalities between 2008 and 2010. Follow-up examinations were performed from June 2011 to March 2013. Changes in the proportion of individuals with abdominal obesity before and after the disaster were compared. Then, lifestyle habits affecting these changes were assessed.Results: We found that 34.2% and 36.6% of participants (P < 0.001), both evacuees (37.0% and 42.1% [P < 0.001]) and non-evacuees (32.8% and 34.0% [P < 0.001]), had abdominal obesity before and after the disaster, respectively. Abdominal obesity was positively associated with smoking cessation, snacking after dinner, and non-breakfast skipping after the disaster and alcohol drinking before and after the disaster (all P < 0.05). Smoking cessation was positively associated with abdominal obesity in both evacuees and non-evacuees and in both men and women (all P < 0.01).Conclusion: The prevalence of abdominal obesity increased among residents in the area affected by nuclear disaster. It might be associated with not only lifestyle habits before the disaster but also changes in these habits after the disaster, especially smoking cessation.

著者

Akira Takagi Satoko Ohfuji Takashi Nakano Hideaki Kumihashi Munehide Kano Toshihiro Tanaka

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

vol.32, no.1, pp.55-56, 2022-01-05 (Released:2022-01-05)

参考文献数

7

著者

Haruka Sato Eri Eguchi Narumi Funakubo Hironori Nakano Hironori Imano Tetsuya Ohira

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

pp.JE20220161, (Released:2022-12-10)

参考文献数

46

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Background: The 2011 Great East Japan Earthquake has resulted in a nuclear accident, forcing residents of the surrounding areas to evacuate. To determine any association between excessive drinking and hypertension in the setting of disaster, we assessed whether the proportion of excessive drinkers increased and if post-disaster excessive drinking was a risk factor for hypertension.Methods: This retrospective study assessed data from the Japanese National Database. Cumulative population data for Fukushima Prefecture (3,497,576 people) were analyzed by categorizing residents into four areas—evacuation, coastal, central, and mountainous—to calculate the proportion of excessive, heavy (equivalent to binge drinking) and at-risk drinkers for 2008–2017. The hazard ratios (HRs) for the incidence of hypertension for 2012–2017 were examined in association with changes in drinking status pre- and post-disaster, which included 136,404 people who received specific health checkups pre-disaster (2008–2010) and post-disaster (2011–2012).Results: The proportion of excessive drinkers among women increased after the disaster in all areas examined. The association between excessive drinking and the incidence of hypertension was determined among men and women in all areas; it was stronger among women in evacuated areas, with the sex- and age-adjusted HRs for the incidence of hypertension of 1.41 for pre-disaster excessive drinking, 2.34 for post-disaster excessive drinking, and 3.98 for pre- and post-disaster excessive drinking, compared with not excessive drinking pre- and post-disaster.Conclusions: Excessive drinking post-disaster may be associated with an increased risk of hypertension among men and women, especially among women in the evacuation areas.

著者

Ko Motoki Yu Kinoshita Ryohei Nakano Munetaka Hosokawa Tetsuya Nakazaki

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.QH-018, (Released:2022-12-15)

被引用文献数

1

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Floral induction by grafting without vernalization treatment (NV grafting method) has potential to shorten breeding times and to diversify the seed production of cabbage, an important leafy vegetable with a long and absolute low temperature exposure requirement for its floral induction. However, it is unknown whether the NV grafting method can be actually used for cabbage breeding and seed production. This is because the NV grafting method’s effect on the field performance of obtained progenies has not been investigated, as opposed to the conventional floral induction method by vernalization treatment. Therefore, in this study we compared the effects of two different floral induction methods on the agricultural traits of the obtained progenies. Two clonal lines of ‘Watanabe-seiko No.1’ cabbage were used for the experiment. In the two-year field experiment, we observed a consistent effect of clonal lines on vegetative growth; however, almost no effects of the floral induction methods on either vegetative or reproductive growth were observed. This was further supported by similar expression levels of FLOWERING LOCUS C homologs in the progenies at the young seedling stage. Pollen production and seed formation of the progenies were confirmed regardless of the floral induction method. In conclusion, cabbage seeds obtained by the NV grafting method are likely to show the same traits as those obtained by the conventional vernalization method. This indicates the direct applicability of this method to cabbage breeding and seed production.

著者

中野 潤三 Junzo NAKANO

雑誌

鈴鹿国際大学紀要Campana = Suzuka International University journal campana (ISSN:13428802)

巻号頁・発行日

vol.21, pp.1-15, 2015-03-10

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The study of security issues during the Cold War era had been centered around national security and on how to protect a nation from external threats using military force. Therefore, the supporters of the current Japanese Constitution and its pacifist ideal have tended to avoid the idea of "national security," which reminds them of the use of military power, and have pitted "pacifism" against it. On the other hand, people who insist Article 9 of the Constitution must be amended, have harshly criticized an absolute pacifism that ignores national security. The pacifism of the Japanese Constitution is a declaration of the renouncement of war, obviously focusing on the classic type of war between states. However, as the concept of security has deepened, I believe that a deeper concept of the Constitution's pacifism should also be developed. If the Constitution were to be amended, I would like to propose adding a statement on "proactive contribution to peace," which would promote human security, to the Preamble to the Constitution. We are faced with a need to improve the entire environment of international security by ensuring a form of human security that helps to prevent terrorism and armed conflicts such as civil war. At the same time, we also need to hold-more than ever-well-balanced discussions dealing with the security of Japan in a practical manner in light of the pacifist ideal of the Japanese Constitution.

著者

Ryo Sasaki Taijun Yunoki Yumiko Nakano Yusuke Fukui Mami Takemoto Ryuta Morihara Koji Abe Toru Yamashita

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

pp.9702-22, (Released:2022-11-23)

参考文献数

17

被引用文献数

2

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Objective During the coronavirus disease 2019 (COVID-19) pandemic, many social activities have moved online using applications for digital devices (e.g. computers, smartphones). We investigated the needs of telemedicine and trends in medical status and social care situations of Japanese patients with neurological disorders in order to estimate their affinity for an online telemedicine application. Methods We designed an original questionnaire for the present study that asked participants what problems they had with hospital visits, how the COVID-19 pandemic had affected their lives, and whether or not they would like to receive telemedicine. Patients The present study included volunteer caregivers, participants with Parkinson's disease (PD), epilepsy, stroke, dementia, immune-mediated neurological disease (IMMD), spinocerebellar degeneration (SCD), amyotrophic lateral sclerosis (ALS), headache, myopathy, and other neurological diseases from Okayama University Hospital. Results A total of 29.6% of patients wanted to use telemedicine. Patients with headaches (60.0%) and epilepsy (38.1%) were more likely to want to use telemedicine than patients with PD (17.8%) or stroke (19.0%). Almost 90% of patients had access to a digital device, and there was no association between favoring telemedicine, ownership of a digital device, hospital visiting time, or waiting time at the hospital, although age was associated with motivation to telemedicine use (52.6 vs. 62.2 years old, p <0.001*). Conclusion We can contribute to the management of the COVID-19 pandemic and the medical economy by promoting telemedicine, especially for young patients with headaches or epilepsy.

著者

Manabu Nitta Makoto Kaneko Sayuri Shimizu Hideaki Kanazawa Yuji Itabashi Kotaro Miura Mike Saji Itaru Takamisawa Morimasa Takayama Shintaro Nakano Saki Hasegawa-Tamba Shinichiro Ueda

出版者

International Heart Journal Association

雑誌

International Heart Journal (ISSN:13492365)

巻号頁・発行日

vol.63, no.5, pp.864-873, 2022-09-30 (Released:2022-09-30)

参考文献数

26

被引用文献数

1

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In patients with atrial septal defect (ASD), atrial left-to-right shunting causes left atrial (LA) remodeling and dysfunction, leading to atrial fibrillation (AF). In adults with ASD and concomitant AF, LA function should be evaluated after ASD closure plus AF radiofrequency catheter ablation (RFCA).This multicenter retrospective cohort study included patients who underwent transcatheter ASD closure at one of the four leading hospitals. Patients with a history of AF also underwent preceding RFCA. The association between AF history and LA ejection fraction (EF) (indicating LA global function) at 6-12 months following ASD closure was evaluated. To account for differences in baseline characteristics between patients with and without a history of AF, we conducted the following statistical methods: (1) multivariate regression analysis in the prepropensity score (PS)-matched cohort and (2) univariate comparisons in the PS-matched cohort.Overall, this study included 231 patients (30 with AF history, 201 without). Multiple regression analysis showed that AF history was independently associated with impaired LAEF (β = −10.425, P < 0.001, model created prior to propensity matching). A one-to-one PS matching (25 pairs) showed that the LAEF at 6-12 months following ASD closure was significantly impaired in patients with ASD and AF history compared to that in patients without history of AF (median LAEF, 37.5% (interquartile range [IQR] 29.4%-48.5%) versus 52.3 [IQR 50.0%-56.6%]; P < 0.001).LA function was impaired in patients with ASD and a history of AF at 6-12 months after successful transcatheter ASD closure and on maintenance of sinus rhythm by RFCA.

著者

Reika Hasegawa Tomoki Arakawa Kenjiro Fujita Yuichiro Tanaka Zen Ookawa Shingo Sakamoto Hironori Takasaki Miho Ikeda Ayumi Yamagami Nobutaka Mitsuda Takeshi Nakano Masaru Ohme-Takagi

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.39, no.2, pp.209-214, 2022-06-25 (Released:2022-06-25)

参考文献数

51

被引用文献数

1

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Brassinosteroid (BR) is a phytohormone that acts as important regulator of plant growth. To identify novel transcription factors that may be involved in unknown mechanisms of BR signaling, we screened the chimeric repressor expressing plants (CRES-T), in which transcription factors were converted into chimeric repressors by the fusion of SRDX plant-specific repression domain, to identify those that affect the expression of BR inducible genes. Here, we identified a homeobox-leucine zipper type transcription factor, BRASSINOSTEROID-RELATED-HOMEOBOX 3 (BHB3), of which a chimeric repressor expressing plants (BHB3-sx) significantly downregulated the expression of BAS1 and SAUR-AC1 that are BR inducible genes. Interestingly, ectopic expression of BHB3 (BHB3-ox) also repressed the BR inducible genes and shorten hypocotyl that would be similar to a BR-deficient phenotype. Interestingly, both BHB3-sx and BHB3-ox showed pale green phenotype, in which the expression of genes related photosynthesis and chlorophyll contents were significantly decreased. We found that BHB3 contains three motifs similar to the conserved EAR-repression domain, suggesting that BHB3 may act as a transcriptional repressor. These results indicate that BHB3 might play an important role not only to the BR signaling but also the regulation of greenings.

著者

Darunmas Sankhuan Meiqiao Ji Sota Takanashi Yuto Imamura Shoichi Sato Kanyaratt Supaibulwatana Masahiro Otani Masaru Nakano

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.39, no.2, pp.205-208, 2022-06-25 (Released:2022-06-25)

参考文献数

14

被引用文献数

2

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LEAFY (LFY), which encodes a plant-specific transcription factor, plays an important role in the transition from vegetative to reproductive development. Ectopic expression of LFY has been reported to induce dwarfism and early flowering in some model plants. In order to examine the possibility of using LFY for molecular breeding of ornamental plants, we produced and characterized transgenic plants ectopically expressing LFY from Arabidopsis thaliana (AtLFY) in the liliaceous ornamental plant Tricyrtis sp. Nine independent transgenic plants have been obtained, and all of them exhibited dwarf phenotypes compared with the vector control. These transgenic plants could be classified into three types according to the degree of dwarfism: one showed an extreamly dwarf phenotype with smaller leaves (Type I); two showed moderately dwarf phenotypes (Type II); and six showed slightly dwarf phenotypes (Type III). All of Type I, Type II and Type III transgenic plants produced flower buds 1–3 weeks earlier than the vector control. Vector control and Type III transgenic plants produced 1–4 apical flower buds, whereas Type I and Type II transgenic plants produced only a single apical flower bud. Type I and Type II transgenic plants often produced non-fully-opened flowers. Quantitative real-time reverse transcription-polymerase chain reaction analysis showed that the AtLFY expression level generally correlated with the degree of dwarfism. These results indicate that morphological alterations observed in the transgenic plants was induced by ectopic expression of AtLFY. Lower levels of ectopic expression of LFY may be valuable for producing dwarf and early flowering ornamental plants.