著者
Taiga Kato Mitsuo Takano Takafumi Nakano Satoshi Shimano
出版者
The Japanese Society of Systematic Zoology
雑誌
Species Diversity (ISSN:13421670)
巻号頁・発行日
vol.28, no.1, pp.23-30, 2023-01-12 (Released:2023-01-12)
参考文献数
44

The taxonomic status of millipedes of the genus Spirobolus Brandt, 1833, referred to as “Yaeyama-maruyasude” from the Yaeyama Islands, Ryukyu Islands, Japan, was unresolved. We assess the taxonomic status of these Yaeyama Spirobolus sp. using an integrated morphological and molecular approach, and describe them as a new species, S. akamma sp. nov., for which partial sequences of the nuclear 28S ribosomal RNA, mitochondrial cytochrome c oxidase subunit I, and 16S ribosomal RNA markers are provided. This new species differs from continental China and Taiwan endemic congeners in anterior gonopod morphology (in having an elongate and subtriangular coxa, and a pentagonal mesal sternal process), posterior gonopod morphology (in having a coronoid prefemoral endite with rounded distal end, and an elongate telopodite), and in having four serrations on the cyphopod lateral flange.
著者
金 玲花 中野 亜里沙 安藤 元一 Kim Ryonghwa Arisa Nakano Motokazu Ando
巻号頁・発行日
vol.59, no.2, pp.137-144,

自由行動ネコが野生鳥獣にどのような捕食圧を与えているか,神奈川県厚木市で調査した。神奈川県自然環境保全センターの傷病鳥獣保護記録を調べたところ,保護鳥獣の10%はネコに襲われたものであり,キジバトやスズメなど地上採餌性の種,あるいはヒヨドリなどの都市鳥が多かった。育雛期である5-7月には鳥類の巣内ヒナが半数以上を占め,ネコの襲いやすい位置に営巣するツバメなどが多かった。同市の住宅地帯および農村地帯におけるアンケート調査では,ネコは13%の世帯で飼育されていた。このうち屋外を自由行動できる飼いネコの比率は,住宅地で29%,農村で59%であり,生息密度に換算すると住宅地で2.2頭/ha,農村で0.35頭/haと推定された。こうしたネコが家に持ち帰る獲物の種類は,住宅地では小鳥と昆虫が多く,農村ではネズミ,小鳥や昆虫など多様であった。持ち帰った獲物の半分以上は食されなかった。これらのネコが年間60頭程度の鳥獣を捕らえると仮定すると,1年に捕食される鳥獣はそれぞれ132頭/ha,21頭/haと推定された。飼いネコによる生態系への影響を避けるためには,室内飼いが望まれる。
著者
Naoto Sawada Haruhiko Toyohara Takafumi Nakano
出版者
The Japanese Society of Systematic Zoology
雑誌
Species Diversity (ISSN:13421670)
巻号頁・発行日
vol.26, no.2, pp.217-224, 2021-09-10 (Released:2021-09-10)
参考文献数
22
被引用文献数
2

Sodium hypochlorite has been used for cleaning specimens of freshwater and brackish water snails that are covered with deposits. Our experiments using specimens of two freshwater snail species, Semisulcospira niponica (Smith, 1876) and S. reticulata Kajiyama and Habe, 1961, showed that this traditional method could remove thin deposit layers, including algae, but was not useful for obstinate deposits. We found that a new method using ammonium thioglycolate could be applied to remove obstinate iron-rich deposits. Though ammonium thioglycolate treatment caused loss of gloss inside the aperture, this loss could be prevented by plugging a kneaded eraser into an aperture. Moreover, the new method could clean specimens with little damage of the periostracum. So as to remove deposits with the least damage to shells, 3% w/v sodium hypochlorite was useful for deposits including algae, and 20% w/v ammonium thioglycolate was suitable for cleaning specimens with iron-rich deposits. Degeneration of the microstructure of inner whorls can be avoided by plugged shell apertures with a kneaded eraser in both methods. Shell deposits that are composed of both algae and iron should be treated first with 20% w/v ammonium thioglycolate, and then with 3% w/v sodium hypochlorite to remove the deposits. Appropriate cleaning methods enable accurate examination and long-term preservation of shell specimens.
著者
Hiroto Nakano Kazuyoshi Yamaguchi Naoyuki Hama Yasuko Matsumoto Moeko Shinohara Hisashi Ide
出版者
The Japanese Society of Internal Medicine
雑誌
Internal Medicine (ISSN:09182918)
巻号頁・発行日
pp.0504-22, (Released:2022-12-28)
参考文献数
14
被引用文献数
5

Anti-myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is an autoimmune demyelinating disorder that mainly occurs post-infection or post-vaccination. MOGAD after inoculation with coronavirus disease 2019 (COVID-19) vaccines is rare, and we herein report a rare case of a patient with MOGAD after vaccination using the Pfizer-BioNTech COVID-19 vaccine (BNT162b2; Pfizer Japan Inc.; Tokyo). Our report highlights the fact that MOGAD following inoculation with COVID-19 vaccine may show clinical relapse during reduction of the oral steroid dose, and continuous treatments with immunological agents is needed to prevent disease recurrence.
著者
KEIYA FUJIMORI HYO KYOZUKA SHUN YASUDA AYA GOTO SEIJI YASUMURA MISAO OTA AKIRA OHTSURU YASUHISA NOMURA KENICHI HATA KOUTA SUZUKI AKIHITO NAKAI MIEKO SATO SHIRO MATSUI KYOKO NAKANO MASAFUMI ABE FOR THE PREGNANCY AND BIRTH SURVEY GROUP OF THE FUKUSHIMA HEALTH MANAGEMENT SURVEY
出版者
福島医学会
雑誌
FUKUSHIMA JOURNAL OF MEDICAL SCIENCE (ISSN:00162590)
巻号頁・発行日
pp.2014-9, (Released:2014-07-15)
参考文献数
29
被引用文献数
8 47

Background: On 11 March 2011, the Great East Japan Earthquake followed by a powerful tsunami hit the Pacific Coast of Northeast Japan and damaged Tokyo Electric Power Company’s Fukushima Daiichi Nuclear Power Plant, causing a radiation hazard in Fukushima Prefecture. The objective of this report is to describe some results of a questionnaire-based pregnancy and birth survey conducted by the Radiation Medical Science Center for the Fukushima Health Management Survey.Materials and Methods: Questionnaires were sent to women who received maternal and child health handbooks from municipal officers in Fukushima Prefecture between 1 August 2010 and 31 July 2011, with the aim of reaching those who were pregnant at the time of the disaster. Mailing began 18 January 2012. Data were analyzed separately for six geographic areas in Fukushima Prefecture.Results: The total number of women meeting survey criteria was 15,972. The number of responses received to date is 9,298 (58.2%). Data from 8602 respondents were analyzed after excluding 634 invalid responses and 5 induced and 57 spontaneous abortions (less than 22 gestational weeks). The incidences of stillbirth (over 22 completed gestational weeks), preterm birth, low birth weight and congenital anomalies were 0.25%, 4.4%, 8.7% and 2.72%, respectively. These incidences are similar to recent averages elsewhere in Japan.Conclusion: Considering the pregnancy and birth survey data in aggregate, our disaster seemed to provoke no significant adverse outcomes over the whole of Fukushima prefecture. But post-disaster prenatal care and support intended for patients’ safety and security should be coupled with ongoing surveillance and rigorous data analysis.
著者
Naruhiko Sunada Hiroyuki Honda Yasuhiro Nakano Koichiro Yamamoto Kazuki Tokumasu Yasue Sakurada Yui Matsuda Toru Hasegawa Yuki Otsuka Mikako Obika Yoshihisa Hanayama Hideharu Hagiya Keigo Ueda Hitomi Kataoka Fumio Otsuka
出版者
The Japan Endocrine Society
雑誌
Endocrine Journal (ISSN:09188959)
巻号頁・発行日
pp.EJ22-0093, (Released:2022-04-28)
被引用文献数
26

Symptoms of long COVID are complex and long-lasting, and endocrine dysfunction might be involved in the underlying mechanisms. In this study, to clarify the hormonal characteristics of long COVID patients, laboratory data for patients who visited the outpatient clinic for long COVID were evaluated. A retrospective analysis was performed for patients who visited Okayama University Hospital during the period from Feb 2021 to Dec 2021 with focus on the interrelationships between major symptoms and endocrine data. Information and laboratory data were obtained from medical records for 186 patients. The patients had various symptoms, and the most frequent symptoms were general malaise, dysosmia/dysgeusia, hair loss, headache, dyspnea, and sleeplessness. Patients who were suffering from fatigue and dysosmia/dysgeusia were younger, while hair loss was more frequent in older and female patients. As for the characteristics of patients suffering from general fatigue, the scores of depression and fatigue were positively correlated with serum levels of cortisol and free thyroxin (FT4), respectively. Also, patients suffering from general fatigue had lower levels of serum growth hormone and higher levels of serum FT4, while patients with dysosmia/dysgeusia had a significantly lower level of serum cortisol. Serum thyrotropin (TSH) levels were higher and the ratios of FT4/TSH were lower in the initially severe cases, suggesting occult hypothyroidism. In addition, the ratios of plasma adrenocorticotropin to serum cortisol were decreased in patients with relatively high titers of serum SARS-CoV-2 antibody. Thus, hormonal changes seem to be, at least in part, involved in the persistent symptoms of long COVID.
著者
KEIYA FUJIMORI HYO KYOZUKA SHUN YASUDA AYA GOTO SEIJI YASUMURA MISAO OTA AKIRA OHTSURU YASUHISA NOMURA KENICHI HATA KOUTA SUZUKI AKIHITO NAKAI MIEKO SATO SHIRO MATSUI KYOKO NAKANO MASAFUMI ABE FOR THE PREGNANCY AND BIRTH SURVEY GROUP OF THE FUKUSHIMA HEALTH MANAGEMENT SURVEY
出版者
福島医学会
雑誌
FUKUSHIMA JOURNAL OF MEDICAL SCIENCE (ISSN:00162590)
巻号頁・発行日
vol.60, no.1, pp.75-81, 2014 (Released:2014-08-08)
参考文献数
29
被引用文献数
8 47

Background: On 11 March 2011, the Great East Japan Earthquake followed by a powerful tsunami hit the Pacific Coast of Northeast Japan and damaged Tokyo Electric Power Company’s Fukushima Daiichi Nuclear Power Plant, causing a radiation hazard in Fukushima Prefecture. The objective of this report is to describe some results of a questionnaire-based pregnancy and birth survey conducted by the Radiation Medical Science Center for the Fukushima Health Management Survey.Materials and Methods: Questionnaires were sent to women who received maternal and child health handbooks from municipal officers in Fukushima Prefecture between 1 August 2010 and 31 July 2011, with the aim of reaching those who were pregnant at the time of the disaster. Mailing began 18 January 2012. Data were analyzed separately for six geographic areas in Fukushima Prefecture.Results: The total number of women meeting survey criteria was 15,972. The number of responses received to date is 9,298 (58.2%). Data from 8602 respondents were analyzed after excluding 634 invalid responses and 5 induced and 57 spontaneous abortions (less than 22 gestational weeks). The incidences of stillbirth (over 22 completed gestational weeks), preterm birth, low birth weight and congenital anomalies were 0.25%, 4.4%, 8.7% and 2.72%, respectively. These incidences are similar to recent averages elsewhere in Japan.Conclusion: Considering the pregnancy and birth survey data in aggregate, our disaster seemed to provoke no significant adverse outcomes over the whole of Fukushima prefecture. But post-disaster prenatal care and support intended for patients’ safety and security should be coupled with ongoing surveillance and rigorous data analysis.
著者
木村 妙子 木村 昭一 自見 直人 倉持 利明 藤田 敏彦 駒井 智幸 吉田 隆太 田中 隼人 岡西 政典 小川 晟人 小林 格 小玉 将史 齋藤 礼弥 清野 裕暉 片平 浩孝 中野 裕昭 吉川 晟弘 上野 大輔 田中 正敦 大矢 佑基 前川 陽一 中村 亨 奥村 順哉 田中 香月 Kimura Taeko Kimura Shoichi Jimi Naoto Kuramochi Toshiaki Fujita Toshihiko Komai Tomoyuki Yoshida Ryuta Tanaka Hayato Okanishi Masanori Ogawa Akito Kobayashi Itaru Kodama Masafumi Saito Masaya Kiyono Yuki Katahira Hirotaka Nakano Hiroaki Yoshikawa Akihiro Uyeno Daisuke Tanaka Masaatsu Oya Yuki Maekawa Yoichi Nakamura Toru Okumura Junya Tanaka Kazuki
出版者
三重大学大学院生物資源学研究科
雑誌
三重大学大学院生物資源学研究科紀要 = The bulletin of the Graduate School of Bioresources Mie University
巻号頁・発行日
vol.45, pp.11-50, 2019-09

Preliminary results of the deep-sea faunal survey conducted from the TR/V Seisui-maru of Mie University in April 2018 are presented. 18 taxonomists and ecologists working on a wide variety of animal taxa participated in this survey. Surveyed areas included the Kumano Sea (off Mie Prefecture) and south of the Kii Strait (off Tanabe Bay, Wakayama Prefecture), at depths of 80-821 m. Sampling gears employed were beam trawl and biological dredge. The collection is represented by macrobenthos and meiobenthos from 11 animal phyla, including arthropods, echinoderms, annelids and molluscans. The number of phyla occurring in each station varied from 3 to 7. The station with most diverse fauna at the phylum level was St. 3B (south of the Kii Strait, 421-543 m depth, sandy mud bottom). Meiofauna includes priapulids and small arthropods, such as ostracods, tanaidaceans, isopods, cumaceans and acarus. In addition to free-living species, parasitic crustaceans, platyhelminthes, acanthocephalans, annelids and cnidarians were also collected from fishes, ascidians, urchins, holothurians, crustaceans and polychaetes. Preliminary identifications are given for Ostracoda, Cirripedia, Amphipoda, Decapoda, Asteroidea,Ophiuroidea, Holothuroidea, polychaetes, Echiura, Mollusca and Xenoturbellida.
著者
Seiji YUKIMOTO Yukimasa ADACHI Masahiro HOSAKA Tomonori SAKAMI Hiromasa YOSHIMURA Mikitoshi HIRABARA Taichu Y. TANAKA Eiki SHINDO Hiroyuki TSUJINO Makoto DEUSHI Ryo MIZUTA Shoukichi YABU Atsushi OBATA Hideyuki NAKANO Tsuyoshi KOSHIRO Tomoaki OSE Akio KITOH
出版者
Meteorological Society of Japan
雑誌
気象集誌. 第2輯 (ISSN:00261165)
巻号頁・発行日
vol.90A, pp.23-64, 2012 (Released:2012-06-07)
参考文献数
157
被引用文献数
354 624

A new global climate model, MRI-CGCM3, has been developed at the Meteorological Research Institute (MRI). This model is an overall upgrade of MRI’s former climate model MRI-CGCM2 series. MRI-CGCM3 is composed of atmosphere-land, aerosol, and ocean-ice models, and is a subset of the MRI’s earth system model MRI-ESM1. Atmospheric component MRI-AGCM3 is interactively coupled with aerosol model to represent direct and indirect effects of aerosols with a new cloud microphysics scheme. Basic experiments for pre-industrial control, historical and climate sensitivity are performed with MRI-CGCM3. In the pre-industrial control experiment, the model exhibits very stable behavior without climatic drifts, at least in the radiation budget, the temperature near the surface and the major indices of ocean circulations. The sea surface temperature (SST) drift is sufficiently small, while there is a 1 W m-2 heating imbalance at the surface. The model’s climate sensitivity is estimated to be 2.11 K with Gregory’s method. The transient climate response (TCR) to 1 % yr-1 increase of carbon dioxide (CO2) concentration is 1.6 K with doubling of CO2 concentration and 4.1 K with quadrupling of CO2 concentration. The simulated present-day mean climate in the historical experiment is evaluated by comparison with observations, including reanalysis. The model reproduces the overall mean climate, including seasonal variation in various aspects in the atmosphere and the oceans. Variability in the simulated climate is also evaluated and is found to be realistic, including El Niño and Southern Oscillation and the Arctic and Antarctic oscillations. However, some important issues are identified. The simulated SST indicates generally cold bias in the Northern Hemisphere (NH) and warm bias in the Southern Hemisphere (SH), and the simulated sea ice expands excessively in the North Atlantic in winter. A double ITCZ also appears in the tropical Pacific, particularly in the austral summer.
著者
金 玲花 中野 亜里沙 安藤 元一 Kim Ryonghwa Arisa Nakano Motokazu Ando
出版者
東京農業大学
雑誌
東京農業大学農学集報 (ISSN:03759202)
巻号頁・発行日
vol.59, no.2, pp.137-144, 2014-09

自由行動ネコが野生鳥獣にどのような捕食圧を与えているか,神奈川県厚木市で調査した。神奈川県自然環境保全センターの傷病鳥獣保護記録を調べたところ,保護鳥獣の10%はネコに襲われたものであり,キジバトやスズメなど地上採餌性の種,あるいはヒヨドリなどの都市鳥が多かった。育雛期である5-7月には鳥類の巣内ヒナが半数以上を占め,ネコの襲いやすい位置に営巣するツバメなどが多かった。同市の住宅地帯および農村地帯におけるアンケート調査では,ネコは13%の世帯で飼育されていた。このうち屋外を自由行動できる飼いネコの比率は,住宅地で29%,農村で59%であり,生息密度に換算すると住宅地で2.2頭/ha,農村で0.35頭/haと推定された。こうしたネコが家に持ち帰る獲物の種類は,住宅地では小鳥と昆虫が多く,農村ではネズミ,小鳥や昆虫など多様であった。持ち帰った獲物の半分以上は食されなかった。これらのネコが年間60頭程度の鳥獣を捕らえると仮定すると,1年に捕食される鳥獣はそれぞれ132頭/ha,21頭/haと推定された。飼いネコによる生態系への影響を避けるためには,室内飼いが望まれる。
著者
中山 泰一 中野 由章 角田 博保 久野 靖 鈴木 貢 和田 勉 萩谷 昌己 筧 捷彦 Yasuichi Nakayama Yoshiaki Nakano Hiroyasu Kakuda Yasushi Kuno Mitsugu Suzuki Tsutomu Wada Masami Hagiya Katsuhiko Kakehi
出版者
情報処理学会
雑誌
情報処理学会論文誌.教育とコンピュータ = IPSJ transactions. TCE
巻号頁・発行日
vol.3, no.2, pp.44-51, 2017-06-14

高等学校情報科の教科担任の現状を明らかにするため,都道府県教育委員会における臨時免許状の授与と,免許外教科担任の許可の状況を調査した.情報科では,臨時免許状や免許外教科担任が他の教科に比べて突出して多用されていることが明らかになった.本論文では,その調査結果を報告するとともに,わが国の情報教育の取り組みについて述べる.
著者
中野 一茂 人見 優子 Kazushige Nakano Hitomi Yuko
巻号頁・発行日
no.26, pp.39-53, 2010-03-31

介護の現場では、2000 年の介護保険法導入を契機に、施設ではより多くの介護福祉士を求め、そのニーズは高まっている。しかし現実には、施設における介護職員の確保は非常に困難な状況にあり、介護職の労働条件・労働環境の整備の劣悪化による離職者が多いと予測される。この介護者の離職問題にふれる場合、「利用者・家族の介護職員に対する暴力」の問題もその関連する要因の一つに考えられる。本研究では、高齢者施設の介護現場において利用者・家族の暴力が関連してどのような問題が生じているのかを把握するために介護職員を対象とした実態調査をおこない、高齢者福祉施設における介護職員の心身に関連した労働環境について考察した。 その結果、施設内で利用者から介護職員が何らかの暴力を振るわれている実態が明らかで、他国をみても特異な結果ではないことが明らかとなった。また何らかの暴力を受けている介護職員は、それを個人的要因あるいは、利用者の疾病に起因するものや自分の中の問題として内包してしまう傾向が強い。内面の自己の価値基準を抑圧することにより、自己の感情を操作し、介護業務を行っている実態があり、感情労働との関連が深かった。またそのことは、離職問題へと発展することが予測された。 今後は、介護業務を感情労働と位置づける研究の蓄積、さらなる実態調査からの結果蓄積による「施設内暴力」の定義・指針作りが急務であると示唆された。
著者
Ko Tomikawa Yuji Abe Takafumi Nakano
出版者
The Japanese Society of Systematic Zoology
雑誌
Species Diversity (ISSN:13421670)
巻号頁・発行日
vol.24, no.2, pp.259-266, 2019-11-25 (Released:2019-11-25)
参考文献数
10
被引用文献数
5

A new species of pseudocrangonyctid amphipod, Pseudocrangonyx uenoi, is described from cave streams in Taga town, central Honshu, Japan. Previous molecular phylogenetic studies reported the taxonomic status of the Taga populations of Pseudocrangonyx as an undescribed species. We present the diagnostic features to discriminate P. uenoi from the other congeners on the basis of morphological study. Pseudocrangonyx uenoi is closely related to P. daejeonensis Lee, Tomikawa, Nakano, and Min, 2018, but clearly distinguished from the latter by the presence of calceoli on female antenna 2, the 5-dentate lacinia mobilis of the left mandible, setation of the peduncular articles of pleopods, the number of articles of rami of the pleopods, a shorter terminal article of uropod 3, and a telson with a deeper cleft.
著者
Naoko Ozawa Naoki Shimojo Yoichi Suzuki Shingo Ochiai Taiji Nakano Yoshinori Morita Yuzaburo Inoue Takayasu Arima Shuichi Suzuki Yoichi Kohno
出版者
Japanese Society of Allergology
雑誌
Allergology International (ISSN:13238930)
巻号頁・発行日
vol.63, no.2, pp.261-266, 2014 (Released:2014-06-06)
参考文献数
18
被引用文献数
20

Background: There are reports that the maternal diet during pregnancy may affect development of babies' eczema. We sought to investigate the association between the maternal diet during pregnancy and the risk of eczema in infancy in Japan. Methods: A birth cohort was set up at 2 hospitals in Chiba city. Dietary habits concerning fish, butter, margarine, yogurt and natto during pregnancy was obtained from mothers just after delivery. The intake frequencies of these foods were classified into four groups: 1) daily, 2) 2-3 times a week, 3) once a week and 4) once a month or less. Diagnosis of eczema at 6 months of age was made by the presence of an itchy rash that persisted more than two months. Results: Valid data on 650 mother-baby pairs were obtained. No relationship between frequencies of the maternal intake of fish, margarine and yogurt during pregnancy and the onset rate of the babies' eczema were observed. For butter consumption, the incidence of babies' eczema was significantly higher in the group with daily intake than in those with an intake 2-3 times a week or less (p = 0.044). For natto, incidence of babies' eczema was significantly lower in the group with everyday intake than those eating it 2-3 times a week or less (p = 0.020). Conclusions: High frequency intake of natto during pregnancy possibly reduces the incidence of eczema in children at 6 months of age.
著者
Mai Takiguchi Tetsuya Ohira Hironori Nakano Yui Yumiya Takayoshi Yamaki Akiomi Yoshihisa Kazuhiko Nakazato Hitoshi Suzuki Tetsuo Ishikawa Seiji Yasumura Kenji Kamiya Yasuchika Takeishi
出版者
International Heart Journal Association
雑誌
International Heart Journal (ISSN:13492365)
巻号頁・発行日
pp.19-110, (Released:2019-10-31)
参考文献数
24
被引用文献数
5

On March 11, 2011, a great earthquake, known as the Great East Japan Earthquake, hit northeastern Japan, resulting in a tsunami that caused a nuclear disaster, the Fukushima Daiichi Nuclear Power Plant accident, forcing about 160,000 people to evacuate. We, therefore, sought to examine the effects of this evacuation on the onset of cardiovascular diseases and sudden death (SD) in Fukushima Prefecture, three years after the earthquake. We divided the evacuation zone into two areas, whole evacuation zone (Area 1) and partial evacuation zone (Area 2), and we defined the north district of the prefecture as the control area (Area 3). We cross-referenced the death certificate data with data from the Fukushima Prefecture acute myocardial infarction registration survey. For each area, we tallied the number of people who fell into the SD, myocardial infarction (MI), and MI suspected groups. We calculated the age-adjusted incidence rates and analyzed the differences in the adjusted incidence rates across three years using a Poisson regression model. The age-adjusted death rate of the SD group was significantly higher in 2011 in all areas than in 2012 or 2013 (P < 0.05). The total death rate was higher in Area 1 in March 2011, just after the disaster, than in the other two areas. The rate of SD was also higher in Area 1 than in the other areas in March 2011. The incidence of sudden cardiac death might have increased just after the Great East Japan Earthquake in the evacuation area, but not in other areas in Fukushima Prefecture.
著者
Yusuke Okazaki Tuyen Thi Nguyen Arisa Nishihara Hisashi Endo Hiroyuki Ogata Shin-ichi Nakano Hideyuki Tamaki
出版者
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
雑誌
Microbes and Environments (ISSN:13426311)
巻号頁・発行日
vol.38, no.1, pp.ME22102, 2023 (Released:2023-03-15)
参考文献数
20
被引用文献数
1

We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10–15‍ ‍kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50‍ ‍ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.
著者
Shinichi Suzuki Shunichi Yamashita Toshihiko Fukushima Keiichi Nakano Sanae Midorikawa Akira Ohtsuru Seiji Yasumura Mitsunori Hosoya Kenji Kamiya Hiroki Shimura Satoru Suzuki Izumi Nakamura Masafumi Abe
出版者
(社)日本内分泌学会
雑誌
Endocrine Journal (ISSN:09188959)
巻号頁・発行日
pp.EJ15-0726, (Released:2016-02-27)
被引用文献数
3 37

After the Fukushima nuclear power plant accident on March 11, 2011, the public of Japan became particularly concerned about the possibility of an increased risk of childhood thyroid cancer, similar to what was observed after the Chernobyl’s accident. Due to serious public health perception, there was an urgency to evaluate the baseline levels of childhood thyroid status in Fukushima prefecture. Therefore we have commenced a thyroid ultrasound examination (TUE) survey of the approximately 360,000 pediatric inhabitants (0 to 18 years of age) who lived in Fukushima at the time of the accident in October 2011. The subjects were divided into three categories according to the standardized diagnostic criteria of ultrasound findings. Category A contained the subjects whose TUE findings were intact or benign. Category B were recommended a confirmatory TUE. Category C was recommended an immediate confirmatory TUE. Results: The survey of 40,302 subjects in the first year was completed in March, 2013. There were 40,097 (99.5%), 205 (0.50%) and 0 subjects in categories A, B and C, respectively. Of the 82 category B subjects who underwent FNAC, 12 were diagnosed with a malignant tumor or were suspected to have malignancy. The 12 subjects received thyroid surgery and 11 thyroid cancers and one benign nodule were confirmed histologically after surgery. This is the first large-scaled TUE survey to employ sophisticated ultrasound screening and aim to evaluate the baseline frequency of childhood thyroid nodules and cysts. The results will become the golden standard of future comparative TUE in Fukushima, Japan.
著者
中野 由章 中山 泰一 Yoshiaki Nakano Yasuichi Nakayama
出版者
情報処理学会
雑誌
情報処理学会第79回全国大会講演論文集
巻号頁・発行日
pp.4-441-4-442, 2017-03-16

情報処理学会第79回全国大会, 2017年3月16日‐3月18日, 名古屋大学東山キャンパス コンピュータと人間社会, 一般セッション, 情報処理教育, 5E-01 (講演日3月17日)