著者

亀岡 弘 鄭 玉珍 宮沢 三雄

出版者

公益社団法人日本薬学会

雑誌

薬学雑誌 (ISSN:00316903)

巻号頁・発行日

vol.96, no.3, pp.p293-298, 1976-03

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An essential oil was obtained in 0.11% yield by steam distillation from the fresh fruit of Canarium album RAEUSCH The oil was treated successively with 5% sodium carbonate and 5% sodium hydroxide solution, and separated into neutral, sodium carbonate-soluble, and sodium hydroxide-soluble fractions. α-Pinene, camphene, β-pinene, myrcene, limonene, 1,8-cineole, p-cymene, linalool, α-copaene, linalyl acetate, cis-p-2-menthen-1-ol, 1-terpinen-4-ol, trans-p-2-menthen-1-ol, β-caryophylene, citronellyl acetate, β-terpineol, α-terpineol, terpinyl acetate, neryl acetate, geranyl acetate, nerol, δ-cadinene, geraniol, δ-cadinol, elemol, 14 kinds of carboxylic acid, 6 kinds of phenolic compound, and alkane of C_<10> to C_<32> were identified from their infrared, mass, and nuclear magnetic resonance spectra, and by chemical methods.

著者

山口 幸洋 任張 幸子 小幡 博子 大河原 知水 江口 裕伸 黒津 敏嗣 鈴木 敬一郎

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.122, no.7, pp.495-498, 2002-07-01

参考文献数

5

被引用文献数

1

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The direct sequencing of PCR products, a bacterial colony (plasmid DNA), or a phage plaque (λDNA) is a very powerful technique in several molecular biological applications. Recently, we reported the successful application of this direct sequencing methodology, called recyclesequencing. Occasionally, however, our sequencing efforts failed due to the presence of agarose gel containing Luria-Bertani (LB) medium. Consequently, we pursued a semiquantitative investigation of the inhibitory effects of agarose and LB medium on the direct sequencing reaction. We found that LB medium concentrations greater than 26.7% inhibited the sequencing reaction. Furthermore, agarose concentrations greater than 0.20% in a reaction mixture also inhibited the sequencing reaction.

著者

菊池 韶彦 廣瀬 忠明 井村 伸正

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.17, no.8, pp.687-692, 1981-08-01

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現代科学の花形の一つである遺伝子工学は人類にとってどのような利益を約束し, また反面どのような危険性をはらんでいるのか.諸外国の事情にも詳しい専門家に期待と問題点を指摘していただき, この手法の主要な応用分野である薬学の関係者が倫理的な面をも含めてこの問題を考える際の参考にしたい.

著者

安本

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.501, pp.810-811, 1923-11

著者

枝野 敏行 新井 晃一 大島 武 古志 朋之 平田 光輝 大口 正夫 岡部 哲郎

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.17, no.3, pp.379-382, 1994-03-15

被引用文献数

4

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In order to identify the membrane-bound peptidase that is responsible for the degradation of endothelin (ET), an endothelin-1 (ET-1) degradation enzyme was solubilized from membrane fractions of porcine kidney with 1% Triton X-100,and subsequently purified by column chromatographies, i. e., diethylamino-Sepharose ion exchange, gel permeation, Con A Sepharose and hydroxyapatite chromatography. On DEAE-Toyopearl ion exchange column chromatography, the ET degradation enzyme and aminopeptidase were separated, but ET degradation enkephalinase activities were not separable. In order to separate ET degradation enzyme and enkephalinase, the active fractions were loaded on each of the column chromatographies : sephacryl S-200,Con A Sepharose or hydroxyapatite. The ET degradation activities were co-migrated with enkephalinase activities on all of the three chromatographies. In addition, the ET degradation activities were inhibited by thiorphan, phosphoramidon and EDTA, which are known to inhibit enkephalinase. These results suggest that ET degradation activity in the membrane fractions of the kidney is related to enkephalinase and may be involved in the degradation of ET-1 in vivo.

著者

亀谷 勝昭

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.19, no.3, pp.140-142, 1973-06-30

著者

西出 喜代治 山村 真理子 小堀 武夫 常本 大英 近藤 聖 佐藤 清

出版者

公益社団法人日本薬学会

雑誌

Chemical & pharmaceutical bulletin (ISSN:00092363)

巻号頁・発行日

vol.36, no.7, pp.2354-2361, 1988-07-25

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The syntheses and the antibacterial activities of new 3-dimethoxyphosphinylmethyl and 3-dihydroxyphosphinylmethyl cephalosporins I-(Z), II-(Z), III-(Z) and III-(E), possessing the chloromethylene or methoxyimino substituent at the α-position to the 7-(2-aminothiazol-4-yl)acetamido or 7-(thiazol-4-yl)acetamido moiety of the cephem nucleus, are described. The key steps of these syntheses were the Michaelis-Arbusov reaction of the 3-halomethylcephem 1 with trimethyl phosphite and the dealkylation reactions of both the dimethoxyphosphinyl group and the p-methoxybenzyl ester of 7a, b-(Z) by treatment with bromotrimethylsilane to afford 9a, b-(Z).

著者

松丸 秀夫 朝比奈 菊雄 加藤 仁

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.14, no.2, pp.117-122, 1978-02-01

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木枯しが吹き出すと落ちつかなくなり, そして雪が降る頃には時々姿を消し, 黒くなった顔で現われる人種, スキーの楽しさにとりつかれた人達-."スキー狂師"であることを自他共に認めておられる薬学人もあちらこちらに数多いようである.そこで, このような方々の間での隠された逸話や思い出話の一端なりとも御被露していただこうと先生方にお集りいただいたが, 出席を予定されていた津田先生には御都合がつかず, 紙上参加の形で思い出話を寄せていただいた.

著者

佐藤 かな子 長井 二三子 青木 直人 西島 基弘

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.120, no.12, pp.1429-1433, 2000-12-01

被引用文献数

5

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Alkyl p-hydroxybenzoates such as isobutyl p-hydroxybenzoate (PHBA-iBu), butyl p-hydroxybenzoate (PHBA-nBu), isopropyl p-hydroxybenzoate (PHBA-iPr), propyl p-hydroxybenzoate (PHBA-nPr), ethyl p-hydroxybenzoate (PHBA-Et), and methyl p-hydroxybenzoate (PHBA-Me) are widely used as preservatives, stabilizers and antiseptics for medical supplies, cosmetics, foodstuffs etc. We determined the binding affinity of alkyl p-hydroxybenzoates to human estrogen receptor α (ERα) and β (ERβ) by non-RI receptor binding assays. PHBA-iBu had a high binding affinity for ERα (IC_<50> : 6.0×10^<-6>M, the relative binding affinity (RBA) : 0.267) and ERβ (IC_<50> : 5.0×10^<-6>M, RBA : 0.340). These IC_<50> values and RBA were almost the same as those of bisphenol A. The ranking of the estrogenic potency of alkyl p-hydroxybenzoates for both ERs is different; that is, PHBA-iBu>PHBA-nBu≒PHBA-iPr≒PHBA-nPr>PHBA-Et≫PHBA-Me. Alkyl p-hydroxybenzoates bound with equal relative affinity to both ERα and β proteins. Alkyl p-hydroxybenzoate having a long alkyl side-chain showed a high affinity for ERα and β. These findings suggest that p-hydroxybenzoates may be endocrine disruptors.

著者

石館 守三 一色 孝 益子 安 細谷 昇

出版者

公益社団法人日本薬学会

雑誌

Pharmaceutical bulletin

巻号頁・発行日

vol.2, no.3, pp.259-262, 1954-09-25

著者

西岡 五夫 松尾 律子 大倉 洋甫 百瀬 勉

出版者

公益社団法人日本薬学会

雑誌

薬学雑誌 (ISSN:00316903)

巻号頁・発行日

vol.88, no.10, pp.1281-1285, 1968-10

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A new colorimetric method for microdetermination of isothiocyanates was established, using 3,6-dinitrophthalic acid as a color developing agent in a potassium carbonatesodium thiosulfate solution. The reaction conditions were examined with allyl isothiocyanate as a model compound. The outline of the procedure is as follows : To 2.0 ml of allyl isothiocyanate solution (10-150 μg/ml), (dissolved in 50% ethylene glycol solution, 1.0 ml of 3,6-dinitrophthalic acid monopyridinium salt solution (0.1%), and 1.0 ml of alkaline solution (40% potassium carbonate and 30% sodium thiosulfate) are added and mixed well. The mixture is heated in a boiling water bath for exactly 10 minutes, cooled in running water, and diluted to 20.0 ml with water. The absorbance of the developed color is read at 400 mμ against the reagent blank. In this color reaction, 3,6-dinitrophthalic acid was assumed to be reduced to 3-amino-6-nitrophthalic acid by S^<2->, which was liberated from allyl isothiocyanate in the alkaline medium.

著者

石井 康子 谷澤 久之 滝野 吉雄

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.108, no.9, pp.904-910, 1988-09-25

被引用文献数

4

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The mechanism of cathartic effect of barbaloin, representative of Aloe, was investigated by using male rats. Barbaloin administered orally was demonstrated to decompose to aloe-emodin-9-anthrone and aloe-emodin in the rat large intestine. And, these decomposed compounds were likely to change each other in the large intestine. Any compound of barbaloin, aloe-emodin-9-anthrone and aloe-emodin administered orally to rats was found to cause an obvious increase of water content in the large intestine, and only aloe-emodin-9-anthrone administered orally caused a significant increase of water content in the small intestine. Furthermore, the clear increase of water content and abnormality of electrolytes (Na^+, K^+) in the colon segment of rat were observed only by aloe-emodin-9-anthrone when compounds tested were injected directly into the colon segment. Therefore, it seemed that aloe-emodin-9-anthrone, a decomposition product of babaloin in the rat large intestine, caused an increase of water content in the large intestine by a different mechanism from the stimulation of peristalsis and this played an important role in cathartic activity of barbaloin.

著者

石館 守三

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.15, no.1, 1979-01-01

著者

鵜飼 貞二

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.15, no.1, 1979-01-01

著者

野副 鉄男

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.15, no.1, 1979-01-01

著者

宮道 悦男

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.15, no.1, 1979-01-01

著者

松本 陽子 今村 主税 伊藤 貴俊 谷口 智穂 上岡 龍一

出版者

公益社団法人日本薬学会

雑誌

Biological & pharmaceutical bulletin (ISSN:09186158)

巻号頁・発行日

vol.18, no.10, pp.1456-1458, 1995-10-15

被引用文献数

15 31

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The hybrid liposomes (90mol% DMPC/10mol% C_<12> (EO)_8 and 90mol% DMPC/10mol% C_<12> (EO)_<12>) have a highly inhibitory action against the growth of tumor cells. The uniform and stable structure of the hybrid liposomes was revealed on the basis of electron microscopy and dynamic light scattering measurements.

著者

竹本 常松 醍醐 皓二 近藤 嘉和 近藤 一恵

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.86, no.10, pp.874-877, 1966-10-25

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The NMR spectrum of domoic acid (in CF_3COOH) indicates signals for a methyl at 1.43 p.p.m. (d;J=6.6 c.p.s.) and at 1.94 p.p.m. (a broad singlet), and no signal for an ethyl group. The methyl signal at 1.94 p.p.m. shows a long-range I-I coupling due to spin decoupling. The NMR spectrum of trimethyl domoate (in CDCl_3) shows a doublet methyl (J=7.0 c.p.s.) at 1.29 p.p.m., a methyl signal which indicates an allylic coupling at 1.79 p.p.m., and an olefinic proton signal corresponding to 3H at 5.5〜6.6 p.p.m. From its first-order analysis, the configuration of the 1,3-hexadienyl side chain was determined as trans : trans : S-trans. The infrared spectra of both domoic acid and trimethyl domoate show absorption due to the trans-double bond. The NMR spectrum of tetrahydrodomoic acid (in CF_3COOH) shows signals for a methyl bonded to a secondary carbon at 1.12 p.p.m. (d;J=6.0 c.p.s.) and at 1.22 p.p.m. (d;J=6.6 c.p.s.). It was concluded from these observations that the structure of domoic acid would be more appropriately represented as L_s-arabo-2-carboxy-4-(1-methyl-5-carboxy-trans : trans : S-trans-1,3-hexadienyl)-3-pyrrolidineacetic acid (VIII) rather than the previously proposed L_s-arabo-2-carboxy-4-(1-methyl-2-carboxy-1,3-hexadieny1)-3-pyrrolidineacetic acid (I).

著者

北村 二朗 嶋 みな子 平塚 京子 浅野 進吾

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.96, no.6, pp.732-736, 1976-06-25

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Examinations were made on the action of anhydromevalonic acid lactone, which inhibits incorporation of mevalonic acid into cholesterol in the cell-free system of rat liver, on microorganisms was studied. Microorganisms examined were mevalonic acid-requiring Lactobacillus acidphilus ATCC 4963 and L. heterohiochii H-1,and Saccharomyces cerevisiae and S. carlsbergensis ATCC 9080 which do not require mevalonic acid and have the ability to synthesize sterol. In the presence of 0.1 μg/ml of mevalonic acid, L. acidophilus required ca. 15 mg/ml of anhydromevalonic acid lactone for 50% suppression and 25 mg/ml for 100% suppression. In the presence of 1.0 or 4.0 μg/ml of mevalonic acid, L. heterohiochii required 0.5 and 20 mg/ml, respectively, of anhydromevalonic acid lactone. In the presence of 15 and 20 mg/ml of anhydromevalonic acid lactone the growth of S. cerevisiae and S. carlsbergensis was completely inhibited. However, there was hardly any effect on the sterol content per unit cell weight.

著者

浦久保 五郎

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.4, no.2, pp.112-116, 1957-03-30