著者
李 賛榮
出版者
大阪歯科学会
雑誌
歯科医学
巻号頁・発行日
vol.53, no.4, pp.g43-g44, 1990

最近のコンポジットレジン修復はエナメル質のみならず, 象牙質に対して接着性を有することが求められている. そこで私は, 新しく試作した4-META系デンチンボンディングシステムの象牙質接着性を検討するとともに, 歯髄に対する病理学的反応をニホンザルを用いて検索し, 臨床応用に際して2, 3の問題点をも指摘することができた. 実験1. 試作ボンディングシステムの象牙質接着性について 試作ボンディングシステムMETAFIL Iを用い, 被験歯は抜去後直ちに4 ℃生理食塩水中に浸漬保存し, 数か月経過のヒト大臼歯を使用した. エッチング剤は20%リン酸溶液 (20-P), 25%リン酸溶液 (25-P), 40%リン酸ゲル (40-P), 10%クエン酸と3%塩化第二鉄混合液 (10-3)と, pH7.4に調整した0.5M・EDTAの5種を用いた. エッチング後のプライマーは, GLUMA (10%Glutaraldehyde+35% Hydroxyethyl-methacrylate) を用いた. コンポジットレジンはClearfil New Bondを用いるときは化学重合型のClearfil Posteriorを, 4-META系の場合は光重合型のMETAFIL Iを用いた. 実験結果 1. 象牙質に対する接着強さは, 一般的に従来の象牙質ボンディング剤に比べて同等かまたはそれ以上の値を示した. 2. 酸処理剤としては40-P以外に10-3が適していた. 3. プライマーとしてのGLUMAとの組み合わせについては, とくに効果をみなかった. 実験2. 試作ボンディングシステムの歯髄反応 用いた修復剤は実験1に用いたものと同様である. ただし, ボンディング剤を適用する前の酸処理については, エナメル質には30-Pを, 象牙質には4.5%EDTA/Fe・Na水溶液を用いた. 実験に供した動物はニホンザル雌雄各5匹 (体重10〜15kg) で, 犬歯を除く上下顎前歯 (総計40歯, 実験歯20, 対照歯20) を使用した. 通法により頬側歯頚部に5級窩洞を形成し, ベベル形成後, 通法に従ってコンポジットレジン充填を施し, 対照歯には水酸化カルシウム製剤およびリン酸亜鉛セメントを使用した. 観察期間は充填後1週間および12週間とした. 反応の程度はADA, FDI/ISOの診断基準に従って確認した. 実験結果 1. 歯髄刺激は従来品のコンポジットレジン修復材と同程度か, それよりやや少ない程度のものであった. 2. 歯髄刺激の反応結果と考えられる修復象牙質の添加が, コントロールに比較してやや多いようであっだが, この原因が歯髄刺激の持続性にあるかどうかは不明で, 重篤な為害作用も認められず, 臨床応用には問題ないと考える.
著者
川島 四郎
出版者
産業評論社
雑誌
菓子とパン
巻号頁・発行日
vol.2, no.1, pp.11-13, 1950-01

1 0 0 0 OA 落語名作揃

著者
落語研究会 編
出版者
盛陽堂
巻号頁・発行日
1912
著者
Thanida SANANMUANG Nawapen PHUTIKANIT Catherine NGUYEN Sukanya MANEE-IN Mongkol TECHAKUMPHU Theerawat THARASANIT
出版者
日本繁殖生物学会
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2012-116, (Released:2013-01-25)
被引用文献数
4 6 1

Developmental competence and quality of in vitro produced embryos has been demonstrated to be lower than in vivo derived embryos. This study aimed specifically to determine the effects of in vitro culture of feline embryos using various culture densities on developmental competence and expression of stress- and apoptotic-related genes in terms of heat shock protein 70 (HSP70) and apoptotic-related (BAX and BCL-2) gene expressions. In experiment 1, we characterized the inducible form of a feline-specific HSP70 mRNA sequence, as it has not been previously reported. The primers for feline HSP70 mRNA were synthesized and tested on heat-treated cat fibroblasts. In experiment 2, feline embryos were cultured at different culture densities (embryo:culture volume; 1:1.25, 1:5 and 1:20). The developmental competence was determined along with HSP70, BAX and BCL-2 transcript abundances using quantitative RT-PCR. In vivo derived embryos were used as a control group. A partial cat HSP70 mRNA sequence (190 bp) was characterized and exhibited high nucleotide identity (93 to 96%) with other species. Cleaved embryos cultured at high density (1:1.25) developed to blastocysts at a lower rate than those generated from lower densities. Irrespective of the culture densities used, in vitro cultured blastocysts showed increased levels of HSP70 and BAX transcripts compared with in vivo counterparts. Blastocysts derived from the highest culture density (1:1.25) showed higher levels of upregulation of HSP70 and BAX transcripts than those cultured at lower culture densities (1:5 and 1:20). In conclusion, increased levels of pro-apoptotic (BAX) and stress-response (HSP70) transcripts correlated with developmental incompetence of embryos cultured at high embryonic density, indicating that stress accumulated during in vitro embryo culture affected the fate for embryo development and quality.
著者
Narong TIPTANAVATTANA Chommanart THONGKITTIDILOK Mongkol TECHAKUMPHU Theerawat THARASANIT
出版者
日本繁殖生物学会
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2012-130, (Released:2013-01-25)
被引用文献数
7 15 3

Spermatogonial stem cells (SSCs) function to regulate the balance of self-renewal and differentiation of male gametes. SSCs have been successfully isolated and cultured in vitro in several species, but not in feline. Therefore, in this study, we aimed to culture and characterize feline SSCs. In experiment 1, testes (n=5) from different pubertal domestic cats were cryosectioned and fluorescently immunolabeled to examine the expression of SSC (GFRα-1), differentiated spermatogonium (c-kit) and germ cell (DDX-4) markers. In experiments 2 and 3, testicular cells were digested and subsequently cultured in vitro. The resultant presumptive SSC colonies were then collected for SSC identification (experiment 2), or further cultured in vitro on feeder cells (experiment 3). Morphology, gene expression and immunofluorescence were used to identify the SSCs. Experiment 1 demonstrated that varying types of spermatogenic cells existed and expressed different germ cell/SSC makers. A rare population of putative SSCs located at the basement membrane of the seminiferous tubules was specifically identified by co-expression of GFRα-1 and DDX-4. Following enzymatic digestion, grape-like colonies formed by 13-15 days of culture. These colonies expressed GFRA1 and ZBTB16, but did not express KIT. Although we successfully isolated and cultured feline SSCs in vitro, the SSCs could only be maintained for 57 days. In conclusion, this study demonstrates, for the first time, that putative SSCs from testes of pubertal domestic cats can be isolated and cultured in vitro. These cells exhibited SSC morphology and expressed SSC-specific genes. However, long-term culture of these putative SSCs was compromised.
著者
田村 健二
出版者
日本社会学会
雑誌
社会学評論 (ISSN:00215414)
巻号頁・発行日
vol.23, no.1, pp.44-52, 1972-07-30

E. Singer ("Identity vs. Identification", in Review of Existential Psychology, 1965, pp. 160173) states that identity is "a personal definition arrived at by attention to and cultivation of individual experience", whereas identification is "a self-definition by adoption, a self-delineation provided by others". On the basis of various theories and experimental data, Singer concludes that identity and identification are mutually incompatible. In my opinion, this conclusion is only partially correct and contains some important oversights with respect to the nature of human development. <BR>The first oversight pertains to the individual experience which he views as central to identity. In most instances of his discussion, he appears to regard individual experience as intra-individual experience which is separate from human relationships. However, I wish to point out that a human being cannot exist in separation from human relationships. That is to say, a human being has both intra-individual experience and joint experience shared with others. He exists by autonomously combining these two kinds of experience. The individual experience of a human being is precisely this autonomous experience which combines these two kinds of experience as its constituents. <BR>A second oversight pertains to the existence of two kinds of identification. The identification referred to by Singer signifies merely the adoption of a self-definition provided by others in joint experience with them. This should be designated as "identification within joint experience", and it certainly is incompatible with identity. However, this joint experience is not the sole type of experience in human relations. Rather, there is experience wherein all component experiences, including the above joint experience, are mutually accepted and contained. Human identity develops mostly in this experience in which all personal individual experiences are stably contained. This principle is often evident in the processes of child development and counselling. Therein we can find that a parent or counsellor, trusted by the self, may provide the self with a more stable, supportive definition. The self then adopts the stable self-definition provided by the other and thus grows more stabilized. It is then that the self, on the basis of this stability, undertakes to acknowledge and accept all of its own experiences genuinely and thus is able to develop its identity. This process should be designated "identification within over-all inclusive experience". Far from being incompatible with identity, it actually fosters identity. <BR>When the components mutually have this over-all, inclusive experience and identity is possessed, a reconstruction of new joint experience takes place. It is here that a true "I and you" solidarity comes into being.
著者
桜井 博
出版者
社団法人日本産科婦人科学会
雑誌
日本産科婦人科學會雜誌 (ISSN:03009165)
巻号頁・発行日
vol.19, no.10, pp.1213-1221, 1967-10-01

最近リンパ系造影法に対する関心が高まり,各方面で活発な研究がなされているが,その読影,副作用,手術前後経過等についての一連の研究は少なく未知の分野が多い.私は教室における婦人性器悪性腫瘍に関する研究の一環として,対照6例を含む64例に計66回の直接的リンパ系造影法と264回の撮影を施行し,その副作用,組織変化及び術前術後診断の関連性等について検討を行なつた.その概要は次の如くである. i)本法実施後の問題となる直接的副作用は37.5℃以上の発熱で,53.1%にみられた.ii)胸管出現した37.5℃以上の発熱群には全例に胸部微細陰影を認め,発熱原因は胸部の微細な油性栓塞であると推定された.iii)対照群のリンパ節造影数は閉鎖節の他はほぼレ線によるリンパ節解剖学に関する成書に記載されている所見と一致した.iv)造影リンパ節数は左側より右側に多く,年令と子宮頚癌の進行期とに関係が認められた.v)リンパ節転移確診例数については,術前レ線診断が組織学的最終診断に最も近い数を示し,確診例数は転移初期像より転移末期像に多かつた.vi)反復リンパ系造影像は初回造影伝と異なり,リンパ管遺残やリンパ節の淡い陰影欠損の傾向が認められた.vii)本法はリンパ系組織を障害し,リンパ節本来の組織像を失わしめるので,生活現象の適応に影響を与えるものと考えられる.なお,読影基準表を作成し,これより直接的リンパ系造影法による進行期別のレ線像を読影し,造影リンパ節数,摘出リンパ節数を算出した報告は本邦において初めてと考える.
著者
鈴木 隆敏
出版者
慶應義塾大学アート・センター
雑誌
Booklet (ISSN:13420607)
巻号頁・発行日
vol.17, pp.74-92, 2009

FUKUZAWA Yukichi 2 : 図版削除1. 「福澤先生は美術を見る目がない」2. 古文化財の海外流出防止へ努力3. 文化財保護は国の仕事4. 「帝室論」で"皇室メセナ"すゝめ5. 「帝室は諸藝術を保護すべし」6. 中年から熱を入れた「漢詩」と「芝居」7. 団十郎に歌舞伎脚本執筆、菊五郎に英語指導8. 福澤家の家庭音楽会
著者
田口 正樹
出版者
北海道大学
雑誌
萌芽研究
巻号頁・発行日
2002

今年度は、前年度に収集した「ドイツ・スイス中世書庫カタログ」と「オーストリア書庫カタログ」の分析を継続するとともに、これらの史料集の刊行以後に公にされたデータやこれらがカバーしていない地域についても情報を収集し、あわせて考察を試みた。調査から浮かび上がった中世後期の学識法蔵書の姿は多様であり、一般的な言明は容易でないが、全体として見ると1450年ごろに変化が生じたように思われる。それ以前は、書庫の所在、蔵書の保有者、蔵書の内容などすべてにおいて教会的性格が顕著であり、法学文献の中心は教会法で、かなりの数見られるローマ法文献もそれと結びつく限りで現れる。蔵書目録の中には、金印勅書や助言学派の助言文献を教会法文献として分類する例もある。一方、1450年以後になると、いくつかの都市で都市参事会の書庫が確立しはじめるとともに、俗人の蔵書も知られるようになり、ローマ法文献の数も以前より増えてくる。またこの時期には、活版印刷術の発展ともおそらく関係して、都市や教会が新たに法学文献の入手を積極的に行う例がいくつか見られる。こうした動きは16世紀にも継続されていき、それまで学識法と最も縁遠かった下級貴族のもとでも、学識法文献が見いだされるに至るのである。以上の整理は、上記史料集がカバーする南・中ドイツだけでなく北ドイツでもおおよそ妥当するように思われる。一方、ドイツ以外の地域としてベルギーの状況と比較すると、ベルギーの方が、ローマ法文献がより早くから豊富に所在するように見えるが、この点はなお詳しい分析を要する。また、法学文献の利用状況を示す史料を余り多く見いだせなかったため、文献の所在とは別にその利用を詳しく解明することは課題として残った。
著者
林 茂子
出版者
東京女子大学
雑誌
英米文学評論 (ISSN:04227808)
巻号頁・発行日
vol.6, no.2, pp.93-127, 1959

Hopkins' poetry strikes us with the beauty and strength of the unique tension with which it overflows. In this essay the writer attempts to consider the nature of this tension in connection with the poet's notion of "self" and his sense of beauty. The poems of nature of the 1870's in which Hopkins admiringly describes beautiful individual things of nature impress the reader with the beauty and strength of tension and give him a feeling of vitality and animation. The present writer considers this tension to spring out when each one of God's creatures expresses its beautiful "self" to the full, unconsciously giving glory to the Creator. The beauty of the tension of self-expression is also perceived in "Harry Ploughman" in which the subject is not nature but a human being. Such is the basic form of Hopkinsian beauty. However, when human beings and therefore consciousness and will are involved, there can be a still higher kind of beauty, the most perfect and ideal of which is seen in Christ's character-unselfish love, magnanimity, tenderness, and the sternness which enabled him to go through the Passion, sacrificing himself for the sake of mankind. It is, in other words, the beauty of the tension of self-exhaustion or self-sacrifice, and this kind of beauty is what most strongly appeals both to the poet's soul and to the poet's senses. The former kind of beauty is, so to say, a beauty at saturation-natural, exhilarating, free, and at ease; while the latter is a beauty beyond saturation, intensely strained, spiritual, often "dangerous," laborious, and pathetic. At the end of this essay, "The Windhover" is referred to as a work in which these two kinds of beauty are contrasted and the poet's aspiration after the highest beauty is most touchingly presented.
著者
百目木剣虹 著
出版者
東亜堂書房
巻号頁・発行日
1918