著者
Masaki TORIMURA Shinya KURATA Kazutaka YAMADA Toyokazu YOKOMAKU Yoichi KAMAGATA Takahiro KANAGAWA Ryuichiro KURANE
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.17, no.1, pp.155-160, 2001 (Released:2005-04-20)
参考文献数
48
被引用文献数
166 304

Fluorescently labeled oligonucleotide probes have been widely used in biotechnology, and fluorescence quenching by the interaction between the dyes and a nucleobase has been pointed out. This quenching causes big problem in analytical methods, but is useful in some other cases. Therefore, it is necessary to estimate the fluorescence quenching intensity under various conditions. We focused on the redox properties of some commercially available fluorescent dyes, and investigated dye-nucleotide interactions between a free dye and a nucleotide in aqueous solution by electrochemical and spectroscopic techniques. Our results suggested that the quenching was accompanied by photoinduced electron transfer between a thermodynamically quenchable excited dye and a specific base. Several kinds of fluorescent dyes labeled to the 5′-end of oligonucleotide C10T6 were prepared, and their quenching ratios compared upon hybridization with the complementary oligonucleotide A6G10. The quenching was completely reversible and their efficiencies depended on the attached fluorophore types. The fluorescence of 5-FAM, BODIPY FL or TAMRA-modified probe was strongly quenched by hybridization.
著者
外岡 豊
出版者
環境技術学会
雑誌
環境技術 (ISSN:03889459)
巻号頁・発行日
vol.29, no.2, pp.84-87, 2000-02-20 (Released:2010-03-18)
参考文献数
16
著者
大森 桂 古泉 佳代 鈴木 智恵美 金子 佳代子
出版者
一般社団法人 日本家政学会
雑誌
日本家政学会誌 (ISSN:09135227)
巻号頁・発行日
vol.59, no.4, pp.221-229, 2008 (Released:2010-07-29)
参考文献数
26
被引用文献数
1

Enhancing the physical activity level has become a significant issue for the Japanese to prevent lifestyle-related diseases. For developing a simple and valid method to assess daily energy expenditure, we measured the energy expended by several physical activities in 13 young Japanese adults using the portable expiration gas metabolic monitor. Each subject wore a heart rate monitor on the chest and three-dimensional accelerometers on the wrist, waist and ankle. The energy expenditure was correlated with the heart rate and with the acceleration at the wrist, waist and ankle. Energy expenditure showed higher correlation coefficients with the acceleration at the waist and ankle than at the wrist. A multiple regression analysis showed that the energy expenditure could be estimated from the accelerations at the wrist and waist, the heart rate, gender, height and weight. The results indicate that measuring both the body acceleration and heart rate was important for developing a simple and valid method to assess daily energy expenditure.