著者

Kin-ya Tomizaki Masaki Obi Hisakazu Mihara

出版者

The Chemical Society of Japan

雑誌

Bulletin of the Chemical Society of Japan (ISSN:00092673)

巻号頁・発行日

vol.85, no.1, pp.69-78, 2012-01-15 (Released:2012-01-15)

参考文献数

23

被引用文献数

2

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We propose a novel, noncompetitive, label-free, and on-chip immunoassay format based on the excluded volume effect by a target antibody that is itself bound to the corresponding epitope on a biochip. The advantage of the present method is that it only requires a biotin-tethering peptide epitope (capture agent) and fluorophore-labeled-avidin (a signal-generating agent) pair for signal readout. The method is based on the interaction between a nonlabeled target antibody and the corresponding peptide epitope of the antigen decelerating the association rate of the fluorophore-labeled avidin to the biotin moiety at the end of the peptide epitope due to the excluded volume effect of the target antibody. This causes significant changes in signal intensity. Without the target antibody, there is no interference in the binding of the signal-generating agent. The use of other specific interactions would allow fine-tuning of the excluded volume and binding rate to the capture agent to improve sensitivity and versatility.

著者

Ryohei KONO Yoshiharu OKUNO Ken-ichi INADA Akihiko TOKUDA Hiroshi HASHIZUME Munehito YOSHIDA Misa NAKAMURA Hirotoshi UTSUNOMIYA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.10, pp.1907-1911, 2011-10-23 (Released:2011-10-23)

参考文献数

35

被引用文献数

19

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Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

著者

Lobna MKAOUAR Yoshifumi ENDO He XI JUN Soo-Jung LEE Tassanee JAROENSONG Manabu MOCHIZUKI Kazuyuki UCHIDA Hiroyuki NAKAYAMA Nobuo SASAKI Takayuki NAKAGAWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112250747, (Released:2012-01-05)

被引用文献数

4 5

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In this study, the nuclear expression of nuclear factor kappa B (NF-κB) in 48 tissues specimens from 25 canine spontaneous mammary gland tumor (MGT) patients were assessed by immunohistochemistry to compare their levels with clinical features, histological types, prognostic outcomes and proliferative activities, including the mitotic index (MI) and cylcinD1 expression. Twelve of eighteen (66.7%) malignant tumor tissues showed a nuclear staining greater than 10%, while benign tumor and hyperplastic tissues showed nuclear staining less than 10%. Higher nuclear expression of NF-κB was positively correlated with larger tumor size, lymph node metastasis and higher MI, however no correlation was observed with distant metastasis and cyclin D1 expression. Higher NF-κB nuclear expression correlated with shorter patient survival. These findings suggest that NF-κB is a useful prognostic factor for canine MGT patients.

著者

In-sok Hwang Juneyoung Lee Dong Gun Lee

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.34, no.10, pp.1602-1608, 2011-10-01 (Released:2011-10-01)

参考文献数

36

被引用文献数

8 13

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Cruciferous vegetables contain glucobrassicin which, during metabolism, yields indole-3-carbinol (I3C). The aim of this study was to find whether indole-3-carbinol caused apoptosis and its mechanism in Candida albicans. We found that treatment of Candida albicans with indole-3-carbinol significantly increased the reactive oxygen species and hydroxyl radical accumulation. The hydroxyl radical is one of the most active components of oxygen, and it is the end product of an oxidative damage cellular death pathway. We investigated the general phenotypes of apoptosis and then investigated whether there were other distinct markers of apoptosis. Furthermore, the effects of thiourea as a hydroxyl radical scavenger and protective effect of trehalose, which is the result of the fungal immune system, was also assured. This study indicates that indole-3-carbinol has apoptosis effects, including a production of hydroxyl radicals, cytochrome c release and activation of metacaspase. Both hydroxyl radicals and metacaspases triggered apoptosis in Candida albicans.

著者

Seung-Kwon Myung Hong Gwan Seo Yoo-Seock Cheong Sohee Park Wonkyong B Lee Geoffrey T Fong

出版者

Japan Epidemiological Association

雑誌

Journal of Epidemiology (ISSN:09175040)

巻号頁・発行日

pp.1112130276, (Released:2011-12-17)

参考文献数

20

被引用文献数

17 24

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Background: Few studies have reported the factors associated with intention to quit smoking among Korean adult smokers. This study aimed to examine sociodemographic characteristics, smoking-related beliefs, and smoking-restriction variables associated with intention to quit smoking among Korean adult smokers.Methods: We used data from the International Tobacco Control Korea Survey, which was conducted from November through December 2005 by using random-digit dialing and computer-assisted telephone interviewing of male and female smokers aged 19 years or older in 16 metropolitan areas and provinces of Korea. We performed univariate analysis and multiple logistic regression analysis to identify predictors of intention to quit.Results: A total of 995 respondents were included in the final analysis. Of those, 74.9% (n = 745) intended to quit smoking. In univariate analyses, smokers with an intention to quit were younger, smoked fewer cigarettes per day, had a higher annual income, were more educated, were more likely to have a religious affiliation, drank less alcohol per week, were less likely to have self-exempting beliefs, and were more likely to have self-efficacy beliefs regarding quitting, to believe that smoking had damaged their health, and to report that smoking was never allowed anywhere in their home. In multiple logistic regression analysis, higher education level, having a religious affiliation, and a higher self-efficacy regarding quitting were significantly associated with intention to quit.Conclusions: Sociodemographic factors, smoking-related beliefs, and smoking restrictions at home were associated with intention to quit smoking among Korean adults.

著者

Swei-Pi WU Cheng-Pin HO Chin-Li YEN

出版者

National Institute of Occupational Safety and Health

雑誌

Industrial Health (ISSN:00198366)

巻号頁・発行日

pp.1110130103, (Released:2011-10-20)

被引用文献数

1

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A wok with a straight handle is one of the most common cooking utensils in the Asian kitchen. This common cooking instrument has seldom been examined by ergonomists. This research used a two-factor randomized complete block design to investigate the effects of wok size (with three diameters - 36 cm, 39 cm and 42 cm ) and handle angle (25°, 10°, -5°, -20°, and -35°) on the task of flipping. The measurement criteria included the maximum acceptable weight of wok flipping (MAWF), the subjective rating and the subjective ranking. Twelve experienced males volunteered to take part in this study. The results showed that both the wok size and handle angle had a significant effect on the MAWF, the subjective rating and the subjective ranking. Additionally, there is a size-weight illusion associated with flipping tasks. In general, a small wok (36 cm diameter) with an ergonomically bent handle (-20°±15°) is the optimal design, for male cooks, for the purposes of flipping.

著者

Dong-In JUNG Ju-Won KIM Hee-Myung PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112210737, (Released:2011-12-28)

被引用文献数

3 6

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A 4-year-old intact female Pekingese dog was presented with ataxia and seizure episodes. Based on magnetic resonance imaging and cerebrospinal fluid analysis results, meningoencephalitis of unknown etiology was suspected. The present case was survived for 1,096 days under cyclosporine plus prednisolone therapy and definitely diagnosed to necrotizing meningoencephalitis. This report describes the clinical findings, serial magnetic resonance imaging characteristics, and pathologic features of a necrotizing meningoencephalitis and long-term survival after cyclosporine with prednisolone therapy.

著者

Chun-Ho PARK Daisuke KOJIMA Hitoshi HATAI Satoshi INOUE Toshifumi OYAMADA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112250744, (Released:2011-12-28)

被引用文献数

4 5

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Traumatic neuroma of the cervical spinal cord was diagnosed in a 14-year-old male mixed-breed dog. A gross view showed two intradural extramedullary masses, measuring 1 cm and 0.6 cm in length and 0.7 cm and 0.4 cm in diameter, attached to the left side of the spinal cord at the level of the sixth and seventh cervical vertebrae. Microscopically, the cervical spinal masses comprised interlacing fascicles of axons and Schwann cells surrounded by collagenous stroma. Immunohistochemically, the fascicles stained positively for neurofilament and S-100 proteins. Ultrastructurally, variably sized myelinated fibers and onion bulb-like structures were observed. To our knowledge, this is the first report of a traumatic neuroma in the cervical spinal cord of a dog.

著者

Suzuki Motofumi Hamachi Kenji Hara Hideki Nakajima Kaoru Kimura Kenji Hsu Chia-Wei Chou Li-Jen

出版者

American Institute of Physics

雑誌

Applied Physics Letters (ISSN:00036951)

巻号頁・発行日

vol.99, no.22, 2011-12

被引用文献数

13

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We have demonstrated that the vapor-liquid-solid (VLS) growth of Ge nanowhiskers is significantly enhanced by high-temperature glancing angle deposition (HT-GLAD). At the substrate temperature of 420 °C, the Ge nanowhiskers grow on the sample deposited at the deposition angle of α = 85°, whereas no long nanowhisker grows on the samples deposited at α ≤ 73°. The kinetic growth model that takes into account the directional incidence of the vapor flux agrees with the experimental results and suggests that the atoms deposited on the side surface of the nanowhiskers play an essential role in the HT-GLAD assisted VLS growth. Supplying the atoms on the side surface of the nanowhiskers is expected to accelerate the growth of the nanowhiskers in any vapor phase growth methods, such as molecular beam epitaxy and chemical vapor deposition.

著者

Min Kyoung SUNG Ki Yong LEE Jun-Bum SHIN Yon Dohn CHUNG

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Information and Systems (ISSN:09168532)

巻号頁・発行日

vol.E95-D, no.1, pp.152-160, 2012-01-01

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Recently, social network services are rapidly growing and this trend is expected to continue in the future. Social network data can be published for various purposes such as statistical analysis and population studies. When social network data are published, however, the privacy of some people may be disclosed. The most straightforward manner to preserve privacy in social network data is to remove the identifiers of persons from the social network data. However, an adversary can infer the identity of a person in the social network by using his/her background knowledge, which consists of content information such as the age, sex, or address of the person and structural information such as the number of persons having a relationship with the person. In this paper, we propose a privacy protection method for social network data. The proposed method anonymizes social network data to prevent privacy attacks that use both content and structural information, while minimizing the information loss or distortion of the anonymized social network data. Through extensive experiments, we verify the effectiveness and applicability of the proposed method.

著者

Jung-ha LEE Jong-hoon LEE Hun-young YOON Na-hyun KIM Jung-hyang SUR Soon-wuk JEONG

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112200732, (Released:2011-12-26)

被引用文献数

5 10

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A six-year-old intact female Maltese dog weighing 3.8 kg presented with a history of mild lameness and swelling on both forelimbs. Radiographic and computed tomographic views revealed an extensive periosteal reaction in all four limbs and a large round mass on the right middle lung lobe. A total lobectomy was performed and pulmonary adenosquamous carcinoma was histologically confirmed. A diagnosis of hypertrophic osteopathy (HO) secondary to a lung tumor was made. Periosteal proliferation decreased significantly after surgery; however, there was evidence of dyspnea, mass recurrence, and periosteal reaction three months post-operatively. This is the first case report of pulmonary adenosquamous carcinoma with HO in a dog in which we describe clinical, imaging, surgical, and histological findings.

著者

WENG Yueh-Hsuan

雑誌

Advanced robotics : the international journal of the Robotics Society of Japan (ISSN:01691864)

巻号頁・発行日

vol.24, no.13, pp.1919-1926, 2010-10-01

著者

Eul-Sang Kim Yang-Sook Ko Junghun Kim Naoko Matsuda-Inoguchi Haruo Nakatsuka Takao Watanabe Shinichiro Shimbo Masayuki Ikeda

出版者

Tohoku University Medical Press

雑誌

The Tohoku Journal of Experimental Medicine (ISSN:00408727)

巻号頁・発行日

vol.200, no.1, pp.7-15, 2003 (Released:2004-10-01)

参考文献数

29

被引用文献数

14 15

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This study was initiated to examine the accuracy of conventional food composition table-based estimation of intakes of energy, protein, lipid and carbohydrate, in comparison with chemical analysis. For this purpose, 66 women (at the ages of 29 to 54 years) in three locations in Jeju Island, Republic of Korea, volunteered to offer 24-hour food duplicate samples. A half of them were house-wives, and the remaining half were farmers or fishers. The duplicate samples were subjected 1) to the chemical analysis for daily intake of energy, protein, lipid and carbohydrate after official methods in Korea (measured values), and 2) to the estimation of intakes of the same items taking advantage of Korean Food Composition Tables (estimated values). The two sets of the results, i.e., the measured and estimated values, were compared by paired and unpaired t-test, and linear regression analysis. The estimated values correlated closely with the measured values, irrespective of energy or the three major nutrients. A close agreement was observed for energy intake (the estimated/measured ratio of >98%), and it was also the case for protein intake (101%). Under- and over-estimation was observed, however, in regard to carbohydrate (by −8%) and lipid intake (by +24%), respectively. It was concluded that the Korean Food Composition Tables are sufficiently accurate when applied for estimation of total energy intake as well as protein intake. Cares should be taken, however, in applying the tables for estimation of lipid and carbohydrate intake, because there may be the risk of over- and under-estimation for the former and the latter, respectively.

著者

Min-Hee KANG Hee-Myung PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112110721, (Released:2011-12-15)

被引用文献数

6 15

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A 5-year-old female Chihuahua dog was presented for acute collapse. Laboratory examinations showed markedly elevated levels of hepatobiliary enzymes. Empiric antibiotic therapy for bacterial infection of the liver was ineffective. The clinical signs worsened with the development of hyponatremia with hypo-osmolality and elevated urine sodium levels. The dog was suspected to have acute cholangiohepatitis associated with an immune-mediated disease. Subsequently, it was diagnosed with syndrome of inappropriate antidiuretic hormone secretion (SIADH) on the basis of the specific disease criteria. Further tests showed normal function of the adrenal and thyroid glands, and MRI and cerebrospinal fluid (CSF) analysis did not show any intracranial diseases. Immunosuppressive therapy and water restriction resolved the clinical signs and improved the SIADH in this dog. This case indicates that SIADH can occur concurrently with suspected immune-mediated liver disease in dogs.

著者

Jung-Hyun KIM Hee-Myung PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112110723, (Released:2011-12-16)

被引用文献数

6 14

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An 8-year old intact female dog was presented with a sudden onset of unilateral hindlimb paralysis of 3 days duration. Based on the history and results of physical, neurological, histopathological examination, and blood work, an arterial thrombosis was suspected as a complication of the hypercoagulability from malignant mammary gland tumor. The thermography provided the evidence of the unilateral femoral thrombus. Initially, thrombolysis with streptokinase administered by intravenous infusion was ineffective. Thereafter, the direct delivery of streptokinase to the site of thrombus was attempted and it was curative. These results suggest that the thermography could describe the site of the arterial thrombus, and local intra-arterial administration of streptokinase may be an effective therapy for the canine arterial thrombosis complicated by malignant mammary gland tumor.

著者

So-Jeung MOON Jong-Hoon LEE Soon-Wuk Jeong Ju-Won KIM Hee-Myung PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112110722, (Released:2011-12-16)

被引用文献数

3 4

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A 7-year-old, 1.76 kg Maltese dog presented with a 4-year history of a chronic pustular lesion and a wet cough. Erosive lesions were seen at the left thoracic wall. Radiology and computed tomography (CT) revealed a bronchocutaneous fistula connecting from the left cranial bronchus to the skin. On definitive surgery, a long wooden toothpick was observed within this tract, and clinical signs resolved after retrieval of the foreign body. Three-dimensional CT was useful to identify the characteristics of the bronchocutaneous fistula However, the wooden foreign body was not apparent on CT. Here, we report the clinical, clinicopathological and diagnostic imaging findings of a chronic bronchocutaneous fistula caused by a foreign body in a dog.

著者

Qun Zhang Tsugunobu Andoh Mitsuhiro Konno Jung-Bum Lee Masao Hattori Yasushi Kuraishi

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.33, no.5, pp.909-911, 2010-05-01 (Released:2010-05-01)

参考文献数

33

被引用文献数

6 10

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In this study, the antipruritic effect of the methanol extract of Ganoderma lucidum (MEGL) was studied in mice. Oral administration of MEGL (10—1000 mg/kg) produced a dose-dependent inhibition of scratching, an itch-related response, induced by intradermal 5-hydroxytryptamine (5-HT) (100 nmol/site), α-methyl-5-HT (100 nmol/site), and proteinase-activated receptor-2 (PAR2)-activating peptide SLIGRL-NH2 (50 nmol/site). However, MEGL (100—1000 mg/kg) did not inhibit the scratching induced by histamine (100 nmol/site), substance P (100 nmol/site), and compound 48/80 (10 μg/site). These results raise the possibility that MEGL is effective against pruritus mediated by proteinases and 5-HT and that primary afferents expressing PAR2 and 5-HT2A receptors are the sites of its action.

著者

Shin-ichi Todoroki

出版者

一般社団法人 電子情報通信学会

雑誌

IEICE Electronics Express (ISSN:13492543)

巻号頁・発行日

vol.8, no.23, pp.1978-1982, 2011 (Released:2011-12-10)

参考文献数

11

被引用文献数

1 7

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The propagation threshold power through a white tight-buffered single-mode optical fiber was found to be 3% less than that through a transparent acrylate-coated fiber. This is because the white pigments in the buffer layer backscatter the visible emission that pumps a fiber fuse. Namely, the backscattered light is absorbed by the thermal decomposition product SiO in the glass melt surrounding the traveling plasma. This self-pumping effect was revealed by comparing the void trains left in fiber segments coated with white or black oil paint. This threshold reduction compels us to re-examine the power tolerance design of optical networks and fiber fuse terminators.

著者

Bo-Ram LEE Su-Hyung LEE Hyekyung LEE Hyunwook KIM Dae-Yong KIM Jihye CHOI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.11, pp.1473-1476, 2011 (Released:2011-12-01)

参考文献数

25

被引用文献数

5 7

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A 14-year-old, mixed breed dog was presented with acute pain and paresis of the hindlimbs. Ultrasonography revealed an intraluminal mass and an abrupt halt of blood flow signal in the distal abdominal aorta. The mass had homogeneous hyperechoic echotexture compared with blood flow. Although clinical presentation suggested a thromboembolism and pituitary dependent hyperadrenocorticism was suspected as a predisposing cause based on adrenal function tests and ultrasonography, an aortic chondrosarcoma originating from the distal abdominal aorta was diagnosed with histologic examination. Primary aortic sarcoma is extremely rare, and extraskeletal chondrosarcoma is only reported in 2 cases previously. Aortic neoplasm should be included in differential diagnosis when an intraluminal aortic mass is observed on ultrasonography and acute paresis of hindlimbs is shown.

著者

手塚 宏史 堀 敦史 O-CarrollFrancis 石川 裕

出版者

一般社団法人情報処理学会

雑誌

情報処理学会研究報告計算機アーキテクチャ(ARC) (ISSN:09196072)

巻号頁・発行日

vol.1998, no.18, pp.25-30, 1998-03-05

参考文献数

20

被引用文献数

8

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我々はPentium Pro 200MHzのPC64台をMyrinetギガビットネットワークによって接続したPCクラスタシステム"RWC PC Cluster II"を構築し,その上にマルチユーザの並列プログラミング環境SCoreを開発している.PCC2上の通信ライブラリPMは通常のメッセージ転送だけでなくリモートメモリライトによるゼロコピーデータ転送をサポートしており,約119Mバイト/秒(Kバイトデータ)のメッセージ転送バンド幅と約109Mバイト/秒(同8Kパイト)のリモートメモリライトバンド幅,および約7.5マイクロ秒(同8バイト)の通信レイテンシを持っている.また,PMのこれらの機能を用いたMPI/PMはPCC2上で約104Mバイト(同1Mバイト)のデータ転送バンド幅と約11マイクロ秒(同8バイト)の通信レイテンシを得ている.MPI/PMを用いたNASパラレルベンチマークの結果によって,PCC2の高い性能とスケーラビリティが実証された.We have built a PC cluster "RWC PC Cluster II" consisting 64 Pentium Pro 200MHz PCs connected by a Myrinet giga-bit network, and have been developing a multi-user parallel programming environment SCore on it. A communication library PM on PCC2 supports a message passing and a remote memory write using zero-copy data transfer. PM achieves 119M bytes/s (8K byte data) message passing bandwidth, 109M bytes/s (8K byte data) remote memory write bandwidth and 7.5 micro second communication latency. MPI/PM that uses these PM facilities achieves 104M bytes/s (1M byte data) data transfer bandwidth and 11 micro second communication latency on PCC2. The NAS parallel benchmark results using MPI/PM have shown PCC2's high performance and scalability.