著者

Jong Kil LEE So Young CHUN Jin Young IM Hee Kyung JIN Tae Gyun KWON Jae-Sung BAE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1203070789, (Released:2012-03-14)

被引用文献数

1 4

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Stem cell based cell therapies offer significant potential for the field of regenerative medicine. Human amniotic fluid stem cells (hAFSCs) are an attractive source for lineage-specific differentiated stem cell therapy since they have properties that are able to differentiate into cells representing all three germ layers. To better understand the fate and location of implanted hAFSCs, a means to monitor cells in living subjects is essential. Here, we showed that differentiated cells, such as neurogenic, endothelial, and myogenic cells, derived from hAFSCs can be effectively labeled by the FITC-incorporated silica-coated nanoparticles, MNPs@SiO2 (FITC), although the labeling efficacy and cytotoxicity were distinct depending on the differentiated cell type. In addition, we observed that MNPs@SiO2-labeled cells provided sufficient signals for detection by optical and confocal microscope imaging when transplanted into the mice. These results suggest that the fluorescent dye incorporated MNPs@SiO2 are a useful tool for the cell labeling and in vivo tracking of differentiated cells derived from hAFSCs.

著者

林 宏作 Hong-zuo LIN 桃山学院大学文学部

出版者

桃山学院大学総合研究所

雑誌

桃山学院大学人間科学 = HUMAN SCIENCES REVIEW, St. Andrew's University (ISSN:09170227)

巻号頁・発行日

no.4, pp.272-258, 1993-03-31

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竹林七賢の巨頭である阮籍は、常にただひとりあてもなく車を駆け、袋小路に行き当たるごとに慟哭して帰ったという逸話がある。本論文は、この逸話の生じた背景を探求すべく、阮籍の魏晋時代における処世観及び思想変遷を分析し、乱世に生きた哲学者の悲劇の解明を試みた。The anecdote is often told of Ruan Ji-the leader of the Seven Sages of the Bamboo Grove-that he was wont to go out alone in his chariot with no particular destination in mind, only to weep bitterly and return to his home when he came up against a blind alley. Through a consideration of the historical background that gave rise to this anecdote, the present paper, by examining Ruan Ji's philosophy of life and the way his thinking changed during the transition from Wei to Jin, seeks to throw light on the tragedy of a philosopher caught up in a world in turmoil.

著者

林 宏作 Hong-zuo LIN 桃山学院大学文学部

雑誌

国際文化論集 = INTERCULTURAL STUDIES (ISSN:09170219)

巻号頁・発行日

no.7, pp.(7)-(19), 1993-02-25

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阮籍の前半生は恰度曹魏が漢帝国を制する時代にあたるが、阮籍は病気などの口実を設け、紛雑した世から逃避し、一時の平静を得ていた。しかし後半生は、司馬氏が益々野心を増大させていった時期にあたり、その司馬昭が阮籍を常に側近とし望み通りに任せたので、司馬王朝のかけがえのない重臣のような存在にみえたが、実は司馬氏にとって阮籍は極めて有効な利用価値のある人間にすぎず、その本心では彼を信じていなかったのである。本論文は史実に基づいて阮籍とその生きた魏晉時代について論じた。Although the first half of Ruan Ji's life coincides precisely with the period when Cao Cao of the Wei kingdom was putting an end to the reign of the Han emperors, Ruan Ji used the pretext of illness to secrete himself away from the troubled world and gain a period of repose. Subsequently, however, he came to be used by the increasingly-ambitious Sima family which had taken defacto control in Wei (and was eventually to establish its own Jin dynasty), and became the advisor of Si-ma Zhao. Nevertheless, although Ruan Ji appeared to play an essential role as a statesman of the kingdom, it is apparent that the Si-ma clan did not genuinely trust him, but merely regarded him as someone with extraordinarily high use-value. The present paper uses historical sources to discuss Ruan Ji and the transition from Wei to Jin through which he lived.

著者

Akihisa Toshihiro Seino Ken-ichi Kaneko Etsuyo Watanabe Kensuke Tochizawa Shun Fukatsu Makoto Banno Norihiro Metori Koichi Kimura Yumiko

出版者

Japan Oil Chemists' Society

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

vol.59, no.1, pp.49-57, 2010

被引用文献数

56

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A new iridoid glycoside, 9-<I>epi</I>-6α-methoxy geniposidic acid (4), three new hemiterpene glycosides, 3-methylbut-3-enyl 2′-O-(β-D-glucopyranosyl)-β-D-glucopyranoside (nonioside K) (6), 3-methylbut-3-enyl 6′-O-(β-D-xylopyranosyl)-β-D-glucopyranoside (nonioside L) (8), and 3-methylbut-3-enyl 6′-O-(β-D-xylofuranosyl)-β-D-glucopyranoside (nonioside M) (9), and two new saccharide fatty acid esters, 6′-O-(β-D-glucopyranosyl)-1′-<I>O</I>-[(2ξ)-2-methylbutanoyl]-β-D-glucopyranose (nonioside N) (16) and 6′-<I>O</I>-(β-D-xylopyranosyl)-1′-<I>O</I>-[(2ξ)-2-methylbutanoyl]-β-D-glucopyranose (nonioside O) (17), were isolated from a methanol extract of the fruits of <I>Morinda citrifolia</I> (noni), along with 11 known compounds, namely, three iridoid glycosides (1-3), two hemiterpene glycosides (5 and 7), and five saccharide fatty acid esters (10-15). Upon evaluation of compounds 1-17 on the melanogenesis in the B16 melanoma cells induced with α-melanocyte-stimulating hormone (α-MSH), 13 compounds (1, 3, 4, 6-14, and 17) exhibited marked inhibitory effects with 34-49% reduction of melanin content at 100 μM with no or almost no toxicity to the cells (91-116% of cell viability at 100 μM).

著者

Ryohei Yamakado Shinri Sugimoto Shin-ichi Matsuoka Masato Suzuki Yasuhiro Funahashi Koji Takagi

出版者

(社)日本化学会

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

vol.41, no.3, pp.249-251, 2012-03-05 (Released:2012-02-25)

参考文献数

18

被引用文献数

9

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m-Calix[3]amide having pyridine on the benzene ring (PyC3A) was synthesized by the cyclization of methyl 3-nonylamino-5-(pyridin-4-yl)benzoate using lithium 1,1,1,3,3,3-hexamethyldisilazide (LiHMDS). The molecular cage 3Pd·2PyC3A was prepared from a 2:3 mixture of PyC3A and [Pd(dppp)(OTf)2] in CDCl3/CD3OD (5/1 in volume). On the other hand, in CDCl3, the formation of a polymeric mixture was confirmed.

著者

沈 煕燦 (2011) 沈 熙燦 (2009-2010)

出版者

立命館大学

雑誌

特別研究員奨励費

巻号頁・発行日

2009

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本研究は、植民地朝鮮に設立された「朝鮮史編修会」という歴史編纂機関を取りあげて、近代史学の特質やその性格を明らかにすることを目的とする。とりわけ、「植民史学の総本山」として評されるのみであって、もっぱら否定と批判の対象としてしか取り扱われなかった「朝鮮史編修会」とその作業を、植民地朝鮮における「実証主義史学」、つまり「近代歴史学」の成立と展開という側面から分析することに力を注いできた。それは今日の日韓における歴史学全般の問題までをも視野におさめる格好の素材でもあると思われるからだ。そのような問題にとり組むため、活溌な史料調査を行った。なかんずく、韓国での現地調査をつうじていまや日韓友好の表象となっている「金忠善/沙也可」が、歴史学においてどのように語られてきたのかを、「朝鮮史編修会」の修史官であった中村栄孝の著作を中心として穿鑿した。また、朝鮮の三大天才とも呼ばれた崔南善の著作を中心として、被植民者が歴史学に託した抵抗の試みとその屈折を綿密に調べた。昨年度(2011年度)は、採用期間の最終年でもあったため、以上の研究成果を含む3年間の蓄積を文章化することに傾注した。とりわけ、いくつかの学会や研究会などで報告を行い、それらの成果を論文としてまとめた。また、昨年7月の『現代思想』の震災に関する臨時特集号に寄稿をも行った。なお、博士論文を年度末に提出し、審査を待っている状況である。

著者

奈良 隆章 川村 卓 島田 一志 Nara Taka-aki Kawamura Takashi Shimada Kazushi

出版者

筑波大学体育センター

雑誌

大学体育研究 (ISSN:03867129)

巻号頁・発行日

no.33, pp.1-10, 2011-03

著者

Yusuke Fukuda Shin-ichiro Miura Keijiro Saku

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.51, no.4, pp.441-442, 2012 (Released:2012-02-15)

参考文献数

1

被引用文献数

1 1

著者

by Gregory R. Choppin Jan-Olov Liljenzin and Jan Rydberg

出版者

Butterworth-Heinemann

巻号頁・発行日

2002

著者

ミノルタカメラ開発センタ-カメラ第3開発部

出版者

写真工業出版社

雑誌

写真工業 (ISSN:03710106)

巻号頁・発行日

vol.56, no.4, pp.27-35, 1998-04

著者

Keun-Chang Kwak

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE Electronics Express (ISSN:13492543)

巻号頁・発行日

vol.6, no.4, pp.187-192, 2009 (Released:2009-02-25)

参考文献数

6

被引用文献数

1

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This paper is concerned with real-time incremental face recognition for home service robots. For this purpose, we use an Incremental Tensor Subspace Analysis (ITSA) to enroll and recognize the user's face through low-price robot camera when robot performs the real-time customized service in home environments. Furthermore, we perform face recognition based on face database constructed in u-robot test bed environments in ETRI (Electronics and Telecommunications Research Institute). Thus it can be used as a core technique in conjunction with Human-Robot Interaction (HRI) that can naturally interact between human and robots in home robot applications. The experimental results on face database reveal that the presented method shows a good performance in comparison with the well-known methods in distant-varying environments.

著者

グル-プ1984年

出版者

文芸春秋

雑誌

文芸春秋

巻号頁・発行日

vol.53, no.12, pp.p94-133, 1975-12

被引用文献数

1

著者

Haruya TAKAHASHI Young-Il KIM Shizuka HIRAI Tsuyoshi GOTO Chie OHYANE Taneaki TSUGANE Chiaki KONISHI Takashi FUJII Shuji INAI Yoko IIJIMA Koh AOKI Daisuke SHIBATA Nobuyuki TAKAHASHI Teruo KAWADA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.8, pp.1621-1624, 2011-08-23 (Released:2011-08-23)

参考文献数

14

被引用文献数

16

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Peroxisome proliferator-activated receptor-α (PPARα) regulates lipid metabolism. We have reported that tomato fruit contains 9-Oxo-(10E,12E)-octadecadienoic acid (9-Oxo-(10E,12E)-ODA), a PPARα agonist. In this study, we found that various tomato samples contained 9-Oxo-(10E,12Z)-ODA and its 13-Oxo-ODA isomers. Furthermore, several isomers showed structural stability under hot and acidic conditions.

著者

Byung-Jae KANG Hak-Hyun RYU Sung-Su PARK Yongsun KIM Heung-Myong WOO Wan Hee KIM Oh-Kyeong KWEON

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1201280766, (Released:2012-02-07)

被引用文献数

16 24

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Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are a promising source of cells for bone tissue engineering. Matrigel is a basement membrane extract containing multiple extracellular components. This mixture may promote the osteogenic differentiation of MSCs and provide a more appropriate microenvironment for the transplanted cells. Here we investigated the effect of Matrigel on the osteogenic potential of Ad-MSCs. Canine Ad-MSCs were cultured in 2D and 3D matrices, and implanted into subcutaneous pouches of dogs either with or without Matrigel. Culture mineralization, cell adhesion efficiency, cell proliferation, osteoid matrix production, and alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase activities were quantified and compared. Ad-MSCs grown in 2D cultures with Matrigel showed higher levels of calcium deposition and ALP activity than those grown in the absence Matrigel under osteogenic conditions. In 3D cultures, the cells cultivated with Matrigel showed greater attachment, proliferation, and osteogenic differentiation than those grown without Matrigel. In vivo, Ad-MSCs implanted with Matrigel showed higher osteogenic potential than those without Matrigel. In conclusion, these data suggest that the use of Matrigel can increase the osteogenic potential of canine Ad-MSCs.

著者

アメングアル・プリエゴ マリア・オルガ (2010-2011) アメングルアル・プリエゴ マリア・オルガ (2009)

出版者

北海道大学

雑誌

特別研究員奨励費

巻号頁・発行日

2009

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脳は自己免疫血栓性疾患である抗リン脂質抗体(APS)の重要な罹患臓器である。通常の動脈硬化性疾患と比べると、APSでは心筋梗塞に比べて脳梗塞が圧倒的に多い。APSの血栓形成機序は、APS患者に存在する抗リン脂質自己抗体が内皮細胞や単球を活性化して向血栓状態にすることと考えられている。本研究では、血管内皮細胞の臓器特異性について注目し、APSにおける脳梗塞の血栓形成機序を解明することによって同疾患の特異的治療法を開発すおること目的とした。本年は材料に、ヒトの脳、肺、皮膚の小血管内皮細胞を用いた。これらの培養細胞に抗リン脂質抗体(ホスファチジルセリン依存性抗プロトロンビン抗体:231D)を加えてインキュベートし、細胞からRNAを抽出してDNAアレイを用いてmRNA発現スクリーニングをおこなった。有意に発現が増加した遺伝子は、PLSCR4,Cenpk,Bhlhb5,Hmgn1,MAF,TNF,TRAF2,NOS3,RPs6ka6,IL-6、VCAM-1などがあった。しかし、これらの遺伝子発現に内皮細胞間の明らかな差はなく、脳由来微小血管内皮細胞に特異的な遺伝子発現上昇はみられなかった。検討した遺伝子発現のうち、抗プロトロンビン抗体によってもっとも有意に増強したのはNOS3(nitric oxide synthase 3)であった。NOS3の発現亢進は、患者由来のポリクローナル抗リン脂質抗体でも確認された。NOS3の遺伝子多型はアルツハイマー病や動脈硬化疾患と関連しており、脳血管障害の病態にNOS3がかかわる可能性がある。今回は抗リン脂質抗体によって誘導される脳血管内皮に特異的な分子は同定できなかったが、NOS3の活性をさらに検討することにより抗リン脂質抗体症候群に伴う脳血管障害のメカニズムを解明できる可能性を考えた。

著者

Nawawi As'ari Ma Chao-mei Nakamura Norio HATTORI Masao KUROKAWA Masahiko SHIRAKI Kimiyasu KASHIWABA Noriaki ONO Minoru

出版者

公益社団法人日本薬学会

雑誌

Biological & pharmaceutical bulletin (ISSN:09186158)

巻号頁・発行日

vol.22, no.3, pp.268-274, 1999-03-15

被引用文献数

3 34

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By screening water and MeOH extracts of 30 Chinese medicinal plants for their anti-herpes simplex virus (HSV)-1 activity, a MeOH extract of the root tubers of Stephania cepharantha HAYATA showed the most potent activity on the plaque reduction assay with an IC_<50> value of 18.0 μg/ml. Of 49 alkaloids isolated from the MeOH extract, 17 alkaloids were found to be active against HSV-1,including 13 bisbenzylisoquinoline, 1 protoberberine, 2 morphinane and 1 proaporphine alkaloids, while benzylisoquinoline and hasubanane alkaloids were inactive. Although N-methylcrotsparine was active against HSV-1,as well as HSV-1 thymidine kinase deficient (acyclovir resistant type, HSV-1 TK^-)and HSV-2 (IC_<50> values of 8.3,7.7 and 6.7 μg/ml, respectively), it was cytotoxic. FK-3000 was found to be the most active against HSV-1,HSV-1 TK^- and HSV-2 (IC_<50> values of 7.8,9.9 and 8.7 μg/ml) with in vitro therapeutic indices of 90,71 and 81,respectively. FK-3000 was found to be a promising candidate as an anti-HSV agent against HSV-1,acyclovir (ACV) resistant-type HSV-1 and HSV-2.

著者

Hidekazu Tanaka Kazuhiro Tatsumi Sei Fujiwara Takayuki Tsuji Akihiro Kaneko Keiko Ryo Yuko Fukuda Kensuke Matsumoto Mayumi Shigeru Akihiro Yoshida Hiroya Kawai Ken-ichi Hirata

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.76, no.2, pp.382-389, 2012 (Released:2012-01-25)

参考文献数

28

被引用文献数

33 38

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Background: Dyssynchrony has various detrimental effects on cardiac function, but its effect on cardiac sympathetic activity is not fully understood. Methods and Results: We studied 50 heart failure patients who underwent cardiac resynchronization therapy (CRT). Cardiac sympathetic activity was assessed by 123I-metaiodobenzylguanidine (123I-MIBG) scintigraphy as the delayed heart-to-mediastinum ratio (H/M ratio). Echocardiography was performed before and 7 months after CRT, and response was defined as a ≥15% decrease in end-systolic volume. Dyssynchrony was determined by the time difference between the anteroseptal-to-posterior wall using speckle-tracking radial strain (≥130ms predefined as significant). H/M ratio in patients with dyssynchrony was less than that in patients without dyssynchrony (1.62±0.31 vs. 1.82±0.36, P<0.05), even though ejection fraction was not significantly different (24±6% vs. 25±7%). Patients with dyssynchrony and H/M ratio ≥1.6 had a higher frequency of response to CRT (94%) and favorable long-term outcome over 3.0 years. In contrast, patients without dyssynchrony and H/M ratio <1.6 were more likely to show a lower frequency of response to CRT (0%) and unfavorable long-term outcome after CRT. Conclusions: Dyssynchrony is associated with cardiac sympathetic activity, and 123I-MIBG scintigraphy may be valuable for predicting the response to CRT. (Circ J 2012; 76: 382-389)

著者

Angelo BASILE Andrea ONETTI-MUDA Konstantinos GIANNAKAKIS Tullio FARAGGIANA Luca ARESU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.12, pp.1669-1675, 2011 (Released:2012-01-06)

参考文献数

48

被引用文献数

1 2

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A juvenile nephropathy in a 4-year-old male Boxer dog, closely resembling the Nephronophthisis (NPHP)-Medullary Cystic Kidney Disease Complex (MCKD) in humans is described. Gross examination of the kidneys revealed several multiple cysts at the corticomedullary junction and in the medulla. Histological examination was characterized by a widespread tubular atrophy and dilatation, with a marked thickening of the tubular basement membrane, interstitial lymphocytic infiltration and fibrosis. Ultrastructural studies revealed dilated tubules with irregular basement membrane thickening and splitting. Lectin histochemistry investigation revealed that the cysts originated in the distal convoluted tubule and collecting duct. Having excluded all other known cystic diseases of the kidney, and based on the lectin histochemistry results, the macroscopic and histological findings of our case are highly compatible with a diagnosis of the NPHP-MCKD complex. To our knowledge, this is the first report describing this particular lesion.

著者

Sungjin CHOI Jongil LEE Kazuyo IGAWA Shigeki SUZUKI Manabu MOCHIZUKI Ryohei NISHIMURA Ung-il CHUNG Nobuo SASAKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.12, pp.1547-1552, 2011 (Released:2012-01-06)

参考文献数

36

被引用文献数

2 2

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Artificial bone implants are often incorporated with osteoinductive factors to facilitate early bone regeneration. Calcium phosphate, the main component in artificial bone implants, strongly binds these factors, and in a few cases, the incorporated proteins are not released from the implant under conditions of physiological pH, thereby leading to reduction in their osteoinductivity. In this study, we coated tailor-made bone implants with trehalose to facilitate the release of basic fibroblast growth factor (bFGF). In an in vitro study, mouse osteoblastic cells were separately cultured for 48 hr in a medium with a untreated implant (T-), trehalose-coated implant (T+), bFGF-incorporated implant (FT-), and bFGF-incorporated implant with trehalose coating (FT+). In the FT+ group, cell viability was significantly higher than that in the other groups (P<0.05). Scanning electron microscopy (SEM) and X-ray diffraction (XRD) revealed that trehalose effectively covered the surface of the artificial bone implant without affecting the crystallinity or the mechanical strength of the artificial bone implant. These results suggest that coating artificial bone implants with trehalose could limit the binding of bFGF to calcium phosphate.

著者

So-Jeung MOON Ju-Won KIM Jung-Hyang SUR Soon-Wuk JEONG Hee-Myung PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.12, pp.1677-1679, 2011 (Released:2012-01-06)

参考文献数

10

被引用文献数

1 4

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A 14-year-old Maltese dog presented for complete medical examination due to intermittent vomiting and diarrhea observed during the previous two days. A single, solitary, lobulated cystic mass was observed in the liver upon ultrasonographic and computed tomographic examination. After surgical hepatic resection to remove the mass, histological examination revealed a multilocular cyst lined by cuboidal to columnar epithelial cells, which is consistent with biliary cystadenoma. Here, we report the clinical, clinicopathological, histopathological, and diagnostic imaging findings of biliary cystadenoma in a dog.