著者

Hiroshi SHIMIZU Takayuki HOSHI Kenji NAKAMURA Jai-Eok PARK

出版者

日本生物環境工学会

雑誌

Environmental Control in Biology (ISSN:1880554X)

巻号頁・発行日

vol.53, no.2, pp.85-88, 2015 (Released:2015-06-17)

参考文献数

9

被引用文献数

4

---

An ultrasonic pollination device was developed to achieve effective artificial pollination of strawberries in artificial light type plant factories. A phase array of hundreds of ultrasonic transducers was appropriately controlled and focused on one point in space, and it is possible to generate a force at any position in space. The force was modulated at 30 Hz which is the characteristic frequency of strawberry flower. The pollination experiment was conducted using the strawberry variety F1 Elan (Fragaria×ananassa). The total weight of the strawberries harvested over 22 days was 1.22 kg, and the average weights of individual strawberry fruits were 9.1 g.

著者

Hirotoshi MIZUKAMI Tsuyoshi SUZUKI Yasuo NAMBO Mutsuki ISHIMARU Hiroshi NAITO Kenji KOROSUE Kentaro AKIYAMA Kenji MIYATA Akira YAMANOBE Kentaro NAGAOKA Gen WATANABE Kazuyoshi TAYA

出版者

日本ウマ科学会

雑誌

Journal of Equine Science (ISSN:13403516)

巻号頁・発行日

vol.26, no.2, pp.49-56, 2015 (Released:2015-07-02)

参考文献数

14

被引用文献数

4 11

---

Development and endocrine changes in Thoroughbreds colts and fillies were compared between those reared at two facilities of the Japan Racing Association, the Hidaka Training and Research Center (Hidaka) and Miyazaki Yearling Training Farm (Miyazaki). Thoroughbred colts and fillies born in Japan between 2003 and 2010 were used. Each colt group and filly group was divided into 2 groups, respectively, and raised in Hidaka or Miyazaki for 7 months from September at 1 year old to April at 2 years old. For the growth parameters, the body weight, height at withers, and girth and cannon circumferences were measured once a month. For parameters of endocrine function, circulating prolactin, luteinizing hormone (LH), follicle-stimulating hormone (FSH), insulin-like growth factor-1 (IGF-1), testosterone, progesterone, and estradiol-17β levels were measured. Regarding growth, the rate of increase over the 7-month period was significantly higher in both colts and fillies raised in Miyazaki than in Hidaka in all 4 parameters: body weight, height at withers, and girth and cannon circumferences. The endocrine changes of the colts and fillies born in 2007 were as follows. In colts, although circulating prolactin tended to be higher in colts reared in Hidaka from October to April, circulating LH, FSH, testosterone, estradiol-17β and IGF-1 tended to be higher in colts reared in Miyazaki than in Hidaka, suggesting that the gonadotropin-releasing hormone-LH/FSH system and the growth hormone-IGF-1 system were more active in colts reared in Miyazaki as compared with those reared in Hidaka. In fillies, circulating prolactin tended to be higher in fillies reared in Hidaka in February and March, but no significant difference was noted in the serum LH, FSH, IGF-1, or progesterone level between the 2 groups. Circulating estradiol-17β tended to be higher in fillies reared in Miyazaki than in Hidaka in October and November. Regarding ovarian function, the initial ovulation occurred by the end of March in 2 (16.7%) of 12 fillies reared in Hidaka and 7 (38.9%) of 18 fillies reared in Miyazaki, suggesting that the ovarian function was more active in fillies reared in Miyazaki as compared with those reared in Hidaka. Based on these findings, it was clarified that development of the body and gonads was faster in Miyazaki compared with Hidaka in both colts and fillies.

著者

Hirose Tetsuro Virnicchi Giorgio Tanigawa Akie Naganuma Takao Li Ruohan Kimura Hiroshi Yokoi Takahide Nakagawa Shinichi Benard Marianne Fox Archa H. Pierron Gerard

出版者

Amer soc cell biology

雑誌

Molecular biology of the cell (ISSN:10591524)

巻号頁・発行日

vol.25, no.1, pp.169-183, 2014-01-01

被引用文献数

360

---

Paraspeckles are subnuclear structures formed around nuclear paraspeckle assembly transcript 1 (NEAT1)/MEN epsilon/beta long noncoding RNA (IncRNA). Here we show that paraspeckles become dramatically enlarged after proteasome inhibition. This enlargement is mainly caused by NEAT1 transcriptional up-regulation rather than accumulation of undegraded paraspeckle proteins. Of interest, however, using immuno-electron microscopy, we find that key paraspeckle proteins become effectively depleted from the nucleoplasm by 50% when paraspeckle assembly is enhanced, suggesting a sequestration mechanism. We also perform microarrays from NEAT1-knockdown cells and find that NEAT1 represses transcription of several genes, including the RNA-specific adenosine deaminase B2 (ADARB2) gene. In contrast, the NEAT1-binding paraspeckle protein splicing factor proline/glutamine-rich (SFPQ) is required for ADARB2 transcription. This leads us to hypothesize that ADARB2 expression is controlled by NEAT1-dependent sequestration of SFPQ. Accordingly, we find that ADARB2 expression is strongly reduced upon enhanced SFPQ sequestration by proteasome inhibition, with concomitant reduction in SFPQ binding to the ADARB2 promoter. Finally, NEAT1(-/-) fibroblasts are more sensitive to proteasome inhibition, which triggers cell death, suggesting that paraspeckles/NEAT1 attenuates the cell death pathway. These data further confirm that paraspeckles are stress-responsive nuclear bodies and provide a model in which induced NEAT1 controls target gene transcription by protein sequestration into paraspeckles.

著者

Shinobu Imai Fumio Saito Hidehito Takase Mitsunobu Enomoto Hiroshi Aoyama Satoshi Yamaji Katsuaki Yokoyama Hiroshi Yagi Toshio Kushiro Atsushi Hirayama

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.72, no.5, pp.709-715, 2008 (Released:2008-04-25)

参考文献数

21

被引用文献数

6 11

---

Background It has been reported that bepridil is as good as amiodarone in converting persistent atrial fibrillation (AF) to sinus rhythm (SR). The conversion effect of bepridil alone is not always satisfactory, however. The efficacy of pharmacological cardioversion by the combination of bepridil and a class Ic antiarrhythmic drug for persistent AF is studied. Methods and Results The participants comprised 37 consecutive patients in whom pharmacological cardioversion was conducted to treat persistent AF (duration 22.5±29.6 months). Each patient first received a class Ia or Ic antiarrhythmic drug, then bepridil alone, then a combined therapy of bepridil at 200 mg/day with a class Ic antiarrhythmic drug at a routine dose. Unaccompanied use of any of the antiarrhythmic drugs achieved pharmacological cardioversion in 14 (38%) of the 37 patients (single therapy group), whereas SR was restored by combination of bepridil and a class Ic antiarrhythmic drug in 22 (combined therapy group) of the remaining 23 patients. The duration of AF was significantly longer in the combined therapy group than in the single therapy group (28.3±31.0 vs 7.3±4.1 months). Conclusion Combined therapy of bepridil and a class Ic antiarrhythmic drug is efficient for pharmacological cardioversion of refractory long-lasting persistent AF. (Circ J 2008; 72: 709 - 715)

著者

今田 純雄 今田 寛 Sumio Imada Hiroshi Imada

雑誌

人文論究 (ISSN:02866773)

巻号頁・発行日

vol.31, no.1/2, pp.99-122, 1981-12-10

著者

大場 裕一 柴田 康平 吉田 宏 OBA Yuichi SHIBATA Kouhei YOSHIDA Hiroshi

出版者

名古屋大学博物館

雑誌

名古屋大学博物館報告 (ISSN:13468286)

巻号頁・発行日

vol.27, pp.13-16, 2011-12-25 (Released:2012-07-18)

---

We found the luminous earthworm, Microscolex phosphoreus (Dugès, 1837), in Nagoya University campus on Jan 2011. This is the first record of this species in Aichi Prefecture. The luminescent mucus was discharged from anus upon mechanical stimulus. The DNA ‘barcode’ sequences of a cytochrome oxidase I region were analysed and compared to those from other localities.

著者

鳥居 大嗣 Torii Hiroshi トリイ ヒロシ

出版者

国際日本学研究会

雑誌

文化/批評 (ISSN:18827411)

巻号頁・発行日

no.6, pp.106-121, 2014-12

---

論文

著者

黒瀨 浩 クロセ ヒロシ Hiroshi KUROSE

雑誌

https://ir.soken.ac.jp/?action=repository_action_common_download&item_id=4084&item_no=1&attribute_id=19&file_no=1

著者

Nobuhiko Mori Hiroshi Horino Akiyoshi Matsugi Noriyuki Kamata Koichi Hiraoka

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.27, no.5, pp.1287-1290, 2015 (Released:2015-05-26)

参考文献数

22

被引用文献数

4

---

[Purpose] We investigated the effect of rhythmic ankle movement on the contralateral soleus H-reflex. The H-reflex was evoked from the right soleus muscle. [Subjects and Methods] Healthy humans rhythmically moved the left ankle (movement condition) or held the left ankle stationary (stationary condition) at one of three positions corresponding to the ankle positions at which the H-reflex was evoked in the movement condition. The background electromyographic amplitude in the right soleus muscle was maintained at 10% of the maximum voluntary contraction level, and that in the right tibialis anterior muscle was matched between the stationary and movement conditions. [Results] The soleus H-reflex was suppressed throughout all phases of contralateral rhythmic ankle movement. [Conclusion] Rhythmic movement of the contralateral joint suppresses the H-reflex in the muscle that is the prime mover of the joint homologous to the rhythmically moving joint. This inhibitory mechanism may be activated during unilateral rhythmic movement to isolate the motor control of the moving ankle from that of the contralateral stationary ankle.

著者

HIROSHI WATANABE MAKIKO YASHIRO TOMOYUKI ASANO SHUZO SATO ATSUSHI TAKAHASHI KYOKO KATAKURA HIROKO KOBAYASHI HIROMASA OHIRA

出版者

福島医学会

雑誌

FUKUSHIMA JOURNAL OF MEDICAL SCIENCE (ISSN:00162590)

巻号頁・発行日

pp.2014-27, (Released:2015-05-19)

参考文献数

17

被引用文献数

4

---

Stressful life situation can trigger the onset and flare-ups of Behçet’s disease (BD). In addition, the association of systemic sclerosis (SSc) and BD is rare. In this study, we report a patient who had Sjögren’s syndrome as a primary disease and subsequently developed SSc and BD after an earthquake disaster and the death of her father.

著者

Sayuri TSURUDA Kouichi AKAKI Hiroshi HIWAKI Akira SUZUKI Hiroshi AKIYAMA

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.76, no.7, pp.1343-1349, 2012-07-23 (Released:2012-07-23)

参考文献数

33

被引用文献数

8

---

A rapid multiplex real-time PCR assay was developed to achieve highly specific, simultaneous detection of two kinds of mushrooms, Omphalotus guepiniformis and Lentinula edodes. Primers and TaqMan minor groove binder probes were designed according to the internal transcribed spacers 1-5.8S region of rDNA and evaluated by the specificity for fruiting bodies of 17 O. guepiniformis, 16 L. edodes and samples from 57 other species. DNA extracts of all the target species had positive signals with no cross-reaction, the limit of detection being 0.00025 ng of DNA. Threshold cycle (Ct) values for raw and processed fruiting bodies and for fruiting bodies (1% (w/w)) mixed with foodstuffs or artificial gastric juice contents ranged from 17.16 to 26.60 for both examined species. This new assay proved specific to the target species, highly sensitive, and applicable to processed food samples and gastric juice contents, making it useful for rapidly identifying O. guepiniformis and L. edodes.

著者

Rui KANO Tomoko TAKAHASHI Takeshi HAYAKAWA Yoshiki YAMAYA Atsuhiko HASEGAWA Hiroshi KAMATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0454, (Released:2015-05-03)

被引用文献数

3 11

---

Feline upper respiratory tract infection due to Aspergillus spp. is considered an emerging disease, with the number of reported cases continuing to rise. In this study, we report the first case of feline sinonasal aspergillosis caused by Aspergillus fischeri in Japan. The patient presented after 2 months of progressive facial deformity around the nose and nasal discharge. The isolate from this case was susceptible to itraconazole (ITZ), voriconazole, and micafungin, but was resistant to amphotericine B. However, the infected cat died approximately 1 month after referral, despite treatment for 12 days ITZ administered orally at 10 mg/kg.

著者

Yasuhisa OISHI Kenji TANI Hiroshi NAKAZAWA Kazuhito ITAMOTO Tomoya HARAGUCHI Yasuho TAURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0469, (Released:2015-04-05)

被引用文献数

12

---

Liver contrast X-ray computed tomography (CT) has been used for evaluation of hepatic vessels for liver transplantation, liver lobectomy, interventional radiology and diagnosis of hepatocellular carcinoma in humans. However, there remains scant available anatomical information on normal hepatic vessels in the veterinary field. In this study, visualization of hepatic vessels was evaluated in 32 normal beagle dogs by X-ray contrast CT using triple phase images. The following hepatic vessels were clearly visualized: arterial, portal and hepatic veins. With regards to the running patterns of the portal vein and hepatic vein, there were no significant differences between the dogs. However, the hepatic artery exhibited some differences in each dog. In particular, the hepatic artery of the quadrate lobe and the right lateral lobe had many running patterns. The results of the present study could be useful for veterinary diagnosis, surgery and interventional radiology.

著者

Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Sakurako NEO Yoko FUJII Yoshito WAKAO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0508, (Released:2015-04-28)

被引用文献数

2

---

Dynamic left ventricular outflow tract obstruction (DLVOTO) is a common condition in cats and humans. In this case report, a dog is described with DLVOTO secondary to severe intra-abdominal hemorrhage caused by a hemangiosarcoma. The dog was a 9-year-old, 35.7-kg, spayed female German Shepard dog that presented with a history of tachypnea and collapse. A Levine II/VI systolic murmur was present at the heart base. Abdominal ultrasonography revealed a splenic mass and a large amount of ascites. Echocardiography showed a reduced left ventricular diameter and an increased aortic velocity caused by systolic anterior motion (SAM) of the mitral valve apparatus. The heart murmur and the SAM were resolved after treatment including a splenectomy and a blood transfusion.

著者

Naoyuki Kizaki Hiroshi Yoshino Hiroaki Kurokawa

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

Nonlinear Theory and Its Applications, IEICE (ISSN:21854106)

巻号頁・発行日

vol.6, no.2, pp.226-236, 2015 (Released:2015-04-01)

参考文献数

19

被引用文献数

1 2

---

The regulatory interactions among genes are summarized by the gene regulatory network. Recently, the gene regulatory network that is described by the differential equations is widely used, and a lot of inference methods using time course data of the gene expression levels have been proposed. One of the successful inference methods of the gene regulatory network is the method using the neural network. In this study, as a method to improve a performance of the gene regulatory network inference using the neural networks, we propose the method to apply a kind of majority rule to the conventional method. Our proposed method infers the regulatory interactions in the gene network based on the results of a lot of trials of the inference using neural networks. In the simulations, we evaluate our proposed method using artificially defined gene regulatory networks. The results show the validity of the proposed method. The results also suggest that the strategy of the proposed method is applicable to various methods using the heuristic solver.

著者

Ludmila Cojocaru Satoshi Uchida Yoshitaka Sanehira Jotaro Nakazaki Takaya Kubo Hiroshi Segawa

出版者

(社)日本化学会

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

pp.150068, (Released:2015-02-17)

被引用文献数

104

---

A compact TiO2 interlayer between FTO and perovskite in planar heterojunction perovskite solar cells is essential for effective charge extraction and minimized recombination. The electrical property of the interface of the perovskite layer with the compact TiO2 layer is thus important for achieving high power conversion efficiency in the perovskite solar cells. The motivation for the present study was to improve cell performance by improving the property of the TiO2/perovskite interface. Interestingly, a remarkable enhancement in cell efficiency was achieved by treatment of the compact TiO2 layer with TiCl4 and UV(O3).

著者

Keisuke SUGIMOTO Yoko FUJII Hiroshi SUNAHARA Takuma AOKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0354, (Released:2015-04-16)

被引用文献数

21

---

Hypertrophic cardiomyopathy (HCM) in cats is characterized by concentric left ventricular (LV) hypertrophy and both diastolic and systolic dysfunction. Although impaired cardiac function detected by tissue Doppler imaging (TDI) in cats with HCM was previously reported, reference ranges of TDI in normal cats and cats with HCM have been reported as widely variable. Two-dimensional speckle tracking echocardiography (STE) was useful for assessment of cardiac function in human patients with HCM, but clinical utility was not known in cats. The aim of this study was to assess global and segmental LV myocardial function using STE in cats with HCM whose TDI variables were within the reference range. A total of 35 cats of different breeds were enrolled in this study. The HCM group (n=22) was cats diagnosed as HCM without left atrial enlargement and with normal TDI measurements. HCM cats were further divided into a segmental hypertrophy (S-HCM) group and a diffuse hypertrophy (D-HCM) group. The control group consisted of 13 clinically healthy cats. No cats in any group showed any clinical symptoms. Conventional echocardiography, TDI, and global and segmental STE indices were evaluated and compared between groups. Only the longitudinal strain rate during early diastole was significantly decreased in both HCM groups, even in all segments including those without hypertrophy in S-HCM group. This study suggests that STE parameters are the more sensitive variables compared with conventional TDI parameters to detect early myocardial diastolic dysfunction in cats with HCM.

著者

Shigeyuki Ohta Sayaka Sakaguchi Yuki Kobayashi Norikazu Mizuno Kenji Tago Hiroshi Itoh

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.38, no.4, pp.594-600, 2015-04-01 (Released:2015-04-01)

参考文献数

26

被引用文献数

1 24

---

GPR56 is a member of the adhesion G protein-coupled receptor (GPCR) and is highly expressed in parts of tumor cells. The involvement of GPR56 in tumorigenesis has been reported. We generated agonistic monoclonal antibodies against human GPR56 and analyzed the action and signaling pathway of GPR56. The antibodies inhibited cell migration through the Gq and Rho pathway in human glioma U87-MG cells. Co-immunoprecipitation analysis indicated that the interaction between the GPR56 extracellular domain and transmembrane domain was potentiated by agonistic antibodies. These results demonstrated that functional antibodies are invaluable tools for GPCR research and should open a new avenue for therapeutic treatment of tumors.

著者

Ryosuke IWAMA Tsubasa SATO Masaaki KATAYAMA Shunsuke SHIMAMURA Hiroshi SATOH Toshihiro ICHIJO Kazuhisa FURUHAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0494, (Released:2015-04-13)

被引用文献数

1

---

We examined the correlation between the glomerular filtration rate (GFR) estimated from an equation based on the serum iodixanol clearance technique and International Renal Interest Society (IRIS) stages of chronic kidney disease (CKD) in cats. The equation included the injection dose, sampling time, serum concentration, and estimated volume of distribution (Vd) of the isotonic, nonionic, contrast medium iodixanol as a test tracer. The percent changes in the median basal GFR values calculated from the equation in CKD cats resembled those of IRIS stages 1–3. These data validate the association between the GFR derived from the simplified equation and IRIS stages based on the serum creatinine concentration in cats with CKD. They describe the GFR ranges determined using single-sample iodixanol clearance for healthy cats and cats with various IRIS stages of CKD.

著者

Kazutoshi Fujibayashi Hirohide Yokokawa Toshiaki Gunji Noriko Sasabe Mitsue Okumura Kimiko Iijima Tomomi Haniu Teruhiko Hisaoka Hiroshi Fukuda

出版者

一般社団法人 日本内科学会

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.54, no.7, pp.717-723, 2015 (Released:2015-04-01)

参考文献数

27

被引用文献数

1 5

---

Objective The aim of this study was to investigate the associations between the incidence of diabetes and the accumulation of markers of impaired glucose metabolism; i.e., pre-diabetes. Methods This retrospective cohort study recruited 1,631 men without diabetes at baseline who attended more than two routine health check-ups at our institution between 2006 and 2012. The participants were divided into four groups based on the number of markers of impaired glucose metabolism exhibited at the initial examination. The following markers of impaired glucose metabolism were defined as risk factors for diabetes: a fasting plasma glucose level of ≥110 mg/dL, 2-hour plasma glucose level of ≥140 mg/dL and glycated hemoglobin (HbA1c) value of ≥6.0% (42 mmol/moL). The risk of developing diabetes was assessed using a multivariate analysis. Results The median examination interval was 1,092 days. The incidence of diabetes rose in association with the number of markers. The subjects with two markers displayed a multivariate-adjusted odds ratio (OR) for diabetes of 19.43 [95% confidence interval (CI): 9.70-38.97] and the subjects with three markers displayed an OR of 48.30 (95% CI: 20.39-115.85) compared with the subjects with one or no markers. Conclusion The present results demonstrate the impact of accumulating markers of impaired glucose metabolism on the risk of developing diabetes. Anti-diabetes intervention strategies should aim to comprehensively assess an individual's risk of developing diabetes at the pre-diabetes stage.