著者
斎藤 章暢 徳丸 雅一 青木 敦子 安藤 佳代子 正木 宏幸 板屋 民子 丸山 務
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.11, no.1, pp.29-33, 1994

<I>Listeria monocytogenes</I> (<I>L. m</I>. ) in shredded cheese was examined for its growth behavior during cold storage and cooking. During storage at 5°C, the concentration after inoculation decreased up to day 20 and thereafter increased. On day 77, the concentration was recovered to almost the same level as that at the time of inoculation, in shredded cheese inoculated with 10<SUP>4</SUP> or 10<SUP>6</SUP>/g <I>L.m</I>. and increased by 1-2 orders in shredded cheese inoculated with 10<SUP>1</SUP>/g <I>L .m</I>. . Also during storage at 10°, the inoculated <I>L.m</I>. decreased up to day 20 and thereafter increased, each bacterial concentration increased by 1-4 orders. Organoleptically, fungigenesis occurred from days 20 and 15 at 5° and 10°, respectively. The number of bacteria surviving in the pizza inoculated with 10<SUP>6</SUP>/g <I>L.m</I>. was 3.6-23/100 g after heating at 200°C for 5 min, while heating at 250°C for 5 min caused the death of all the inoculated bacteria, that is 10<SUP>6</SUP>/g. The change of the temperature at the center of the pizza while cooking tended to decrease with the increases in the temperature of storage and in the volume of ingredients, and these changes were also macroscopically recognizable.<BR>The above findings suggested that <I>L. m</I>. in shredded cheese dose not proliferate during the allowable period of storage before consumption and becomes extinct with general methods of cooking.
著者
川瀬 雅雄 吉岡 丹 細谷 美佳子 紫竹 美和子
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.32, no.1, pp.48-53, 2015-03-31 (Released:2015-09-10)
参考文献数
9
被引用文献数
2

Even after Kudoa septempunctata parasitic in Olive flounder was found as a cause of food poisoning, similar cases of unknown cause were still taking place. Juvenile Pacific bluefin tuna (JPBT, hereafter) is often presented in those cases and it is suspected that K. hexapunctata parasitic in JPBT would be pathogenic for humans. We attempted to detect K. hexapunctata from JPBT leftover and patients' feces in 4 cases in Niigata prefecture from 2011 to 2014. In addition, we also investigated excretion of K. hexapunctata into the feces after intake of JPBT on a trial basis. The 4 cases occurred during summer from July to September. K. hexapunctata was detected from JPBT leftover and patients' feces; therefore, it was estimated that K. hexapunctata could be parasitic in JPBT eaten in all those cases. Furthermore, we also found that JPBT and Olive flounder were eaten together in 2 cases, but K. septempunctata was not detected from patients' feces. The detection rate of K. hexapunctata from patients' feces was 86% for feces collected within 3 days after meal, but it was lowered 25% for feces collected after 1 week. The quantity of K. hexapunctata excreted into feces after intake of JPBT on a trial basis tended to be declining with time, and then K. hexapunctata was detected in feces up to approximately 3 days after intake of JPBT. From those results, a fecal examination would be important as collecting feces after meal or during an early stage, and feces collected within 3 days after meal could be appropriate for the examination. Cases in relation to JPBT are similar to those of food poisoning caused by K. septempunctata and it is suspected that K. hexapunctata might be the cause. For K. hexapunctata, it would be necessary to accumulate and examine the case data while clarifying its pathogenicity.
著者
鈴木 淳
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.29, no.1, pp.65-67, 2012-03-30 (Released:2012-08-23)
参考文献数
7
被引用文献数
3 2
著者
久田 孝 矢野 俊博
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.27, no.4, pp.185-195, 2010-12-31 (Released:2011-01-17)
参考文献数
45
被引用文献数
1 3
著者
林 志直
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.27, no.3, pp.109-114, 2010-09-30 (Released:2010-12-13)
参考文献数
7
被引用文献数
1 3
著者
小西 典子 甲斐 明美 松下 秀 野口 やよい 高橋 由美 関口 恭子 新井 輝義 諸角 聖 小久保 彌太郎
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.18, no.1, pp.9-14, 2001-03-31 (Released:2010-07-12)
参考文献数
15
被引用文献数
3 4

1998年8月から11月の間に有機.水耕栽培野菜の汚染実態調査を行った.卸売市場およびスーパーマーケットで買い上げた大根37件, 人参29件, キャベツ28件, ネギ28件, レタス32件, キュウリ29件, トマト40件, 玉ネギ28件, カイワレ大根32件, アルファルファ13件, およびサラダ菜, ホウレン草, モヤシ各1件の合計299件を対象とし, 生菌数, 大腸菌, 腸管出血性大腸菌O157およびサルモネラについて検査した.その結果, 生菌数は1g当たり10個以下から107個までと広範囲にわたっていた.特にアルファルプァとカイワレ大根の生菌数は全体に高かった.大腸菌はネギ1件より検出された.O157は検出されなかったが, サルモネラはアルファルファ1件より検出された.検出されたサルモネラはO13群血清型Cubanaであった.アルファルファ由来株と同時期に検出された同血清型ヒト保菌者由来株との関連性を薬剤感受性試験, プラスミドプロファイルおよびXbaI , SpeIで消化後のPFGEパターンの比較により検討した.その結果PFGEパターンが異なっていたことから, 両者の関連性は否定された.
著者
池 晶子 左納 明日香
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.40, no.2, pp.57-62, 2023-06-30 (Released:2023-06-29)
参考文献数
12

Nukadoko, a mixture of rice bran, salt, and water, is used for pickling vegetables to make Japanese traditional Nukazuke. Some Nukadoko manufacturers remove the bran-derived bacteria via heat treatment, but the effects of this treatment on the fermentation process are poorly understood. Herein, we investigate the effects of heat treatment and determine the contribution of bran-derived microorganisms to fermentation. We monitored pH and the counts of lactic acid bacteria (LAB), viable bacteria and yeasts in commercially unheated and heat-treated Nukadoko during the three 1-week fermentation periods. In heat-treated Nukadoko, the pH did not decrease during the first round of pickling. The LAB counts after the third fermentation round in heat-treated Nukadoko were substantially lower than those in unheated Nukadoko. External addition of LAB, Enterococcus faecalis, Leuconostoc mesenteroides, and Lactobacillus plantarum, to heat-treated Nukadoko did not accelerate the lactic acid fermentation of the first pickling. Native LAB in rice bran seemed to play an important role in the fermentation startup. During long-term refrigeration of Nukadoko without pickling, LAB and viable bacterial counts, and pH decreased; however, yeast counts increased. In heat-treated Nukadoko, such microbiological changes during storage were less than those observed in unheated Nukadoko.
著者
伊藤 寛 童 江明
出版者
Japanese Society of Food Microbiology
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.11, no.3, pp.151-157, 1994-12-20 (Released:2010-07-12)
参考文献数
22
被引用文献数
8 7
著者
小川(晴気) 七菜 塚原 拓也 加田 茂樹 宮本 真理
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.40, no.1, pp.26-35, 2023-03-31 (Released:2023-04-18)
参考文献数
37

Bifidobacterium globosum is expected to be applied to foods as a probiotic, and a method to rapidly distinguish it from the related Bifidobacterium pseudolongum is necessary for product development and quality control. In this study, we evaluated existing methods of bacterial species classification for B. globosum/pseudolongum with the published whole-genomes. The results showed that the Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (RFLP) method by the restriction enzyme HaeIII for hsp60 genes and the sequence analysis of hsp60 and rpoB gene sequences could not accurately distinguish B. globosum/pseudolongum, and both species could be accurately distinguished by the sequence analysis of clpC gene sequences. The clpC gene sequence similarity was at least 97.9% in the same species and no more than 95.4% in different species. Therefore, we developed a novel method of RFLP by restriction enzyme BspT104I on PCR products of clpC genes to identify B. globosum/pseudolongum. This method was considered to be faster and more accurate than previously proposed methods for identification of B. globosum/pseudolongum. Thus, the method presented in this study enables efficient species classification and it is very useful in utilization of B. globosum/pseudolongum for foods.
著者
高田 瞬 馬場 洋一 岡元 満 柿澤 広美 伊藤 武
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.34, no.1, pp.40-44, 2017-03-30 (Released:2017-04-10)
参考文献数
15

筆者らは多数の食品従事者の糞便を対象に生物発光酵素免疫法(BLEIA法)に基づく高感度な全自動免疫測定システムと遺伝子検査法(RT-PCR法)の感度,特異性,検出率について検討し,本システムの有効性を評価した.今回対象とした2,427例中ノロウイルスの陽性率はBLEIA法が7.3%,RT-PCR法が6.3%であった.BLEIA法はRT-PCR法に対して,相対感度95.4%,相対特異度98.7%,一致率98.4%であったことから遺伝子検査法と同等の性能を有することが示唆された.BLEIA法は遺伝子検査法と比べて操作性と検査時間を大きく改善する方法であり,1検体あたり46 minでの迅速な測定ができ,処理能力は1 hrあたり120検体を可能としている.高いスクリーニング性能を有していることから,食品従事者の定期検査等の大量の検査に導入して,コスト,時間の削減につながることが期待できる.
著者
久田 孝
出版者
日本食品微生物学会
雑誌
日本食品微生物学会雑誌 (ISSN:13408267)
巻号頁・発行日
vol.32, no.1, pp.29-33, 2015-03-31 (Released:2015-09-10)
参考文献数
9
被引用文献数
1