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1 0 0 0 OA Protective effect of vitamin C against double-strand breaks in reconstituted chromatin visualized by single-molecule observation

著者: Yoshikawa Yuko Hizume Kohji Oda Yoshiko Takeyasu Kunio Araki Sumiko Yoshikawa Kenichi
出版者: Biophysical Society
雑誌: Biophysical Journal (ISSN:00063495)
巻号頁・発行日: vol.90, no.3, pp.993-999, 2006-02
被引用文献数: 36

Direct attack to genomic DNA by reactive oxygen species causes various types of lesions, including base modi. cations and strand breaks. The most significant lesion is considered to be an unrepaired double-strand break that can lead to fatal cell damage. We directly observed double-strand breaks of DNA in reconstituted chromatin stained by a fluorescent cyanine dye, YOYO (quinolinium, 1, 1 '-[1, 3-propanediylbis[(dimethyliminio)-3, 1- propanediyl]]bis[4-[(3-methyl-2(3H)-benzoxazolylidene) methyl]]-, tetraiodide), in solution, where YOYO is known to have the ability to photo-cleave DNAs by generating reactive oxygen species. Reconstituted chromatin was assembled from large circular DNA (106 kbp) with core histone proteins. We also investigated the effect of vitamin C ( ascorbic acid) on preventing photo-induced double-strand breaks in a quantitative manner. We found that DNA is protected against double-strand breaks by the addition of ascorbic acid, and this protective effect is dose dependent. The effective kinetic constant of the breakage reaction in the presence of 5 mM ascorbic acid is 20 times lower than that in the absence of ascorbic acid. This protective effect of ascorbic acid in reconstituted chromatin is discussed in relation to the highly compacted polynucleosomal structure. The results highlight the fact that single-molecule observation is a useful tool for studying double-strand breaks in giant DNA and chromatin.

2012-01-09 09:22:27
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1 0 0 0 OA commencer à inf. について

著者: 安達博明 Hiroaki Adachi
雑誌: 人文論究 (ISSN:02866773)
巻号頁・発行日: vol.54, no.2, pp.87-98, 2004-09-25

2012-01-08 20:48:37
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http://hdl.handle.net/10236/6232

1 0 0 0 OA ブータンに学ぶ観光開発の哲学 : GNHとツーリズムの関係性についての一考察

著者: 山村高淑
出版者: 日本交通公社
雑誌: 観光文化
巻号頁・発行日: vol.188, pp.18-21, 2008-03-20

2012-01-08 20:32:03
1 + 1 Twitter

http://hdl.handle.net/2115/34651

1 0 0 0 OA Antinuclear Energy Movements in Germany and Japan : A Comparative Analysis of Protest against Disposal of Nuclear Waste

著者: Sato Nagako
出版者: 大阪大学大学院国際公共政策研究科
雑誌: 国際公共政策研究 (ISSN:13428101)
巻号頁・発行日: vol.14, no.1, pp.175-189, 2009-09

2012-01-07 00:51:38
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http://hdl.handle.net/11094/10065

1 0 0 0 OA 自然公園における利用者規制の環境経済学的分析:北海道雨竜沼湿原を事例として

著者: 庄子康栗山浩一
出版者: 北海道大学農学部演習林
雑誌: 北海道大学農学部演習林研究報告 (ISSN:03676129)
巻号頁・発行日: vol.55, no.2, pp.369-414, 1998-09

2012-01-06 19:50:46
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http://hdl.handle.net/2115/21444

1 0 0 0 OA Vapor-liquid-solid growth of Ge nanowhiskers enhanced by high-temperature glancing angle deposition

著者: Suzuki Motofumi Hamachi Kenji Hara Hideki Nakajima Kaoru Kimura Kenji Hsu Chia-Wei Chou Li-Jen
出版者: American Institute of Physics
雑誌: Applied Physics Letters (ISSN:00036951)
巻号頁・発行日: vol.99, no.22, 2011-12
被引用文献数: 13

We have demonstrated that the vapor-liquid-solid (VLS) growth of Ge nanowhiskers is significantly enhanced by high-temperature glancing angle deposition (HT-GLAD). At the substrate temperature of 420 °C, the Ge nanowhiskers grow on the sample deposited at the deposition angle of α = 85°, whereas no long nanowhisker grows on the samples deposited at α ≤ 73°. The kinetic growth model that takes into account the directional incidence of the vapor flux agrees with the experimental results and suggests that the atoms deposited on the side surface of the nanowhiskers play an essential role in the HT-GLAD assisted VLS growth. Supplying the atoms on the side surface of the nanowhiskers is expected to accelerate the growth of the nanowhiskers in any vapor phase growth methods, such as molecular beam epitaxy and chemical vapor deposition.

2012-01-06 17:28:25
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1 0 0 0 OA フィクションとは何か

著者: 河田学
出版者: 京都大学
巻号頁・発行日: 2005-05-23

新制・課程博士

2012-01-06 11:21:36
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http://hdl.handle.net/2433/144569

1 0 0 0 OA <大学の研究・動向>シミュレーションで探るジオスペース : 宇宙環境利用に向けて

著者: 大村善治海老原祐輔
出版者: 京都大学電気関係教室・洛友会
雑誌: Cue : 京都大学電気関係教室技術情報誌 (ISSN:18825214)
巻号頁・発行日: vol.26, pp.3-8, 2011-09

2012-01-06 11:00:49
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1 0 0 0 OA バビロニアの数学 : 楔形文字で書かれた古代数学 (数学史の研究)

著者: 室井和男
出版者: 京都大学数理解析研究所
雑誌: 数理解析研究所講究録 (ISSN:18802818)
巻号頁・発行日: vol.1064, pp.160-171, 1998-10

2012-01-06 01:43:38
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http://hdl.handle.net/2433/62440

1 0 0 0 OA A・シュッツの社会科学方法論の構想

著者: 河野憲一
出版者: 早稲田大学大学院文学研究科
雑誌: 早稲田大学大学院文学研究科紀要. 第1分冊, 哲学東洋哲学心理学社会学教育学 = Bulletin of the Graduate Division of Letters, Arts and Sciences of Waseda University. I, Philosophy, oriental philosophy, psychology, sociology, education (ISSN:13417517)
巻号頁・発行日: vol.49, pp.125-137, 2003

2012-01-05 18:50:00
1 はてなブックマーク

http://hdl.handle.net/2065/8440

1 0 0 0 OA 表紙 : 表紙の説明

著者: 岡村定矩
出版者: 東京大学理学部
雑誌: 東京大学理学部廣報
巻号頁・発行日: vol.24, no.3, pp.1-2, 1993-03

銀河団A3528のCCD画像

2012-01-04 22:36:34
1 + 1 Wikipedia

http://hdl.handle.net/2261/28024

1 0 0 0 OA 楕円曲線の岩澤理論における木田の公式について(代数的整数論とその周辺)

著者: 八森祥隆松野一夫
出版者: 京都大学数理解析研究所
雑誌: 数理解析研究所講究録 (ISSN:18802818)
巻号頁・発行日: vol.1026, pp.43-62, 1998-02

2012-01-04 20:17:00
1 はてなブックマーク

http://hdl.handle.net/2433/61771

1 0 0 0 OA 六世紀における上毛野の前方後円墳規格系列

著者: 山田俊輔
出版者: 早稲田大学大学院文学研究科
雑誌: 早稲田大学大学院文学研究科紀要. 第4分冊, 日本史東洋史西洋史考古学 = Bulletin of the Graduate Division of Literature of Waseda University. IV, Japanese history, oriental history, western history, archaeology (ISSN:13417541)
巻号頁・発行日: vol.48, pp.81-91, 2002

2012-01-04 17:37:00
1 知恵袋

http://hdl.handle.net/2065/8589

1 0 0 0 OA 認知過程のコネクショニスト・モデル

著者: 河原哲雄
出版者: 東京大学大学院教育学研究科
雑誌: 東京大学大学院教育学研究科紀要 (ISSN:13421050)
巻号頁・発行日: vol.36, pp.227-238, 1996-12-20

10 years have passed since the 'Bible'of the prallel distributed processing was published (Rumelhart, McClelland & PDF Research Group, 1986). Throughout the decade, architectures and procedures of connectionist (a.k.a neural nets or parallel distributed processing) models have been growing more and more subtle and complex. The aim of this article is to review some recent connectionist models for three typical research domains of higher cognitive processes (language, analogical inference, and category learning) in detail, and to speculate on their impacts on the coming decade of cognitive psychology and connectionism.