著者

Yong-Qiang Shan Yan-Ping Zhu Jing Pang Yan-Xiang Wang Dan-Qing Song Wei-Jia Kong Jian-Dong Jiang

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.36, no.10, pp.1562-1569, 2013-10-01 (Released:2013-10-01)

参考文献数

41

被引用文献数

11 40

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This study was designed to improve the absorption and hypoglycemic efficacy of berberine (BBR), which is a substrate of P-glycoprotein (P-gp), by combination with a P-gp inhibitor tetrandrine (Tet). Flow cytometry and LC-MS/MS were used to determine the cellular efflux or retention of chemicals. Pharmacokinetic study was performed in ICR mice following oral administration of the study compounds. The hypoglycemic efficacies of the compounds were evaluated in diabetic KK-Ay mice. In the in vitro experiments, Tet significantly inhibited the efflux and increased the uptake of P-gp substrates rhodamine-123 as well as BBR in MCF7/DOX cells and Caco-2 intestinal cells. Meanwhile, Tet greatly reduced the expression of P-gp in Caco-2 cells. The inhibition of BBR efflux by Tet was translated into improved pharmacokinetics in vivo. When co-administered, Tet dose-dependently increased the average maximum concentration (Cmax) and area under concentration–time curve (AUC0–24) of BBR in mice. Tet itself had no impact on glucose metabolism. However, it greatly potentiated the hypoglycemic efficacy of BBR in diabetic KK-Ay mice. In addition, we found that Tet had moderate inhibitory effect on the catalytic activity of CYP3A4, which played a role in the bio-transformation of BBR, and this may also take part in the improvement of the pharmacokinetics of BBR. In summary, combination with P-gp inhibitors such as Tet can improve the pharmacokinetics and hypoglycemic efficacy of BBR greatly; this implicates a feasible strategy for exploring the therapeutic effects of BBR and other pharmaceuticals which are substrates of P-gp.

著者

par Seiti Ino-ouet

出版者

F. Carré

巻号頁・発行日

1881

著者

Ji-Young Kim Tae-Heon Oh Byeong Jin Kim Sang-Suk Kim Nam Ho Lee Chang-Gu Hyun

出版者

公益社団法人 日本油化学会

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

vol.57, no.11, pp.623-628, 2008 (Released:2008-10-04)

参考文献数

20

被引用文献数

10 35

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In this study, the chemical composition and anti-inflammatory activities of hydrodistilled essential oil from Farfugium japonicum were investigated for the first time. The chemical constituents of the essential oil were further analyzed by GC-MS and included 1-undecene (22.43%), 1-nonene (19.83%), β-caryophyllene (12.26%), α-copaene (3.70%), γ-curcumene (2.86%), germacrene D (2.69%), and 1-decene (2.08%). The effects of the essential oil on nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages were also examined. The results indicate that the F. japonicum essential oil is an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the F. japonicum essential oil were accompanied by dose-dependent decreases in the iNOS and COX-2 mRNA expression. In order to determine whether F. japonicum essential oil can safely be applied to human skin, the cytotoxic effects of F. japonicum essential oil were determined by colorimetric MTT assays in human dermal fibroblast and keratinocyte HaCaT cells. F. japonicum essential oil exhibited low cytotoxicity at 100 μg/mL. Based on these results, we suggest that F. japonicum essential oil may be considered a potential anti-inflammatory candidate for topical application.

著者

Long Zhe Guo Tae-Hee Kim Seongho Han Sung-Whan Kim

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-15-1169, (Released:2016-02-05)

参考文献数

28

被引用文献数

2 6

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Background:Although stem cells have been regarded as a promising therapeutic option, the marginal therapeutic effects of stem cells are limitations that must be overcome for the development of effective cell therapy. This study sought to identify the angio-vasculogenic properties of endothelial differentiated mesenchymal stem cells (MSCs) and to determine whether these cells are effective for vascular repair.Methods and Results:Adipose MSCs were cultured for 10 days under endothelial cell (EC) culture conditions. These endothelial cell differentiated adipose MSCs (EA) and undifferentiated adipose MSCs (UA) were characterized via angiogenesis and adhesion assays. These cells were transplanted into a hindlimb ischemia (HLI) model to determine therapeutic effects and their underlying mechanisms. EA displayed low adhesion and angiogenic properties in vitro compared with UA. When implanted into mouse HLI models, EA exhibited the decreased recovery of blood perfusion in limb ischemia than uncultured UA. Histology data showed that injected EA exhibited lower retention, angiogenic cytokine levels, and neovascularization in vivo than did UA. Short-term differentiated EA display less cell engraftment and angio-vasculogenic potential, and are less effective for peripheral vascular repair than UA.Conclusions:EC differentiation of MSCs may not present an effective strategy for the promotion of therapeutic neovascularization.

著者

Sayaka Masada-Atsumi Yukie Kumeta Yutaka Takahashi Takashi Hakamatsuka Yukihiro Goda

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.37, no.3, pp.454-460, 2014-03-01 (Released:2014-03-01)

参考文献数

23

被引用文献数

5 18 3

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Despite the increasing sales of black cohosh (the dried rhizome and root of Cimicifuga racemosa L.) in the world herbal market, these products have continuous adulteration issues. The botanical authenticity of the black cohosh products is the first important step for ensuring their quality, safety and efficacy. In this study, we genetically identified the botanical sources of 10 black cohosh products and 5 Cimicifuga Rhizome crude drugs of Japanese Pharmacopoeia grade, and analyzed the metabolic profiling of 25 black cohosh products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, we found that C. dahurica and possibly C. foetida are misused as sources of the black cohosh products and in some cases, the extracts of black cohosh were adulterated with the plant materials of C. dahurica. We demonstrated that these three species can be distinguished by three marker compounds in a specific mass range. These results must be helpful in establishing regulations for the safe use of the black cohosh products.

著者

MENG Channarith LEON-GONZALEZ Roberto

出版者

GRIPS Policy Research Center

雑誌

GRIPS Discussion Papers

巻号頁・発行日

vol.15-22, 2016-01

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While earlier studies focus on credit booms in advanced and emerging market countries, this paper examines the characteristics and determinants of credit booms in developing countries. The results find that credit booms in developing countries are less likely to be associated with systemic banking crises. Rather, they are more likely to be the result of financial deepening than of dangerous buildups of financial risks; the prevention of credit booms in developing countries may thus be associated with higher opportunity costs in terms of foregone growth opportunities. Random effect probit and tobit regressions find some evidence that size of financial system and favorable macroeconomic conditions are among the determinants of credit booms. Although monetary and fiscal policies do not help in preventing credit booms in developing countries, we find that prudential regulations and supervision can play a much more effective role in preventing “bad” booms, while incurring substantially lower costs. Although “bad” booms are hard to identify ahead of time, the duration and size of booms, as well as the level of credit aggregates, appear to be useful indicators in determining them.

著者

Henri-Frédéric Amiel [introduction by Edmond Scherer]

出版者

Manz

巻号頁・発行日

0000

著者

Henri-Frédéric Amiel précédés d'une étude par Edmond Scherer

出版者

Fischbacher

巻号頁・発行日

1922

著者

Haruka Wada Hiroya Kudo Hajime Sasaki Muhammad Baghdadi Ken-ichir Seino

出版者

日本炎症・再生医学会

雑誌

Inflammation and Regeneration (ISSN:18809693)

巻号頁・発行日

vol.35, no.5, pp.238-243, 2015 (Released:2015-12-15)

参考文献数

17

被引用文献数

3

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Recent progress of manipulating pluripotent stem cells expands possibilities of regenerative medicine and opens novel transplantation medicine. However, in many cases of these medicines, the relationship between therapeutic cells and recipients would be allogeneic. In this context, we proposed new concept of immune regulation therapy in new-age medicine using pluripotent stem cells. In our concept, not only grafts but also immune regulating cells are generated from pluripotent stem cells by exertion of its pluripotency. We have recently developed immune suppressive macrophage-like cells from pluripotent stem cells. These cells suppressed allo-antigen stimulated T cell proliferation in an iNOS dependent manner. Furthermore, these immune suppressive macrophage-like cells derived from pluripotent stem cells prolonged survival of grafts derived from same pluripotent stem cells in allogeneic recipients. Thus, series of our study proved the efficacy of our new immune regulating strategy in the age of regenerative medicine which utilize pluripotent stem cells as a therapeutic cell source.

著者

HYUN-JIN LEE IN-KOO RHEE KYUNG-BOK LEE ICK-DONG YOO KYUNG-SIK SONG

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.53, no.7, pp.714-719, 2000-07-25 (Released:2008-09-19)

参考文献数

28

被引用文献数

24 36

著者

JI-SOOK HWANG KYUNG-SIK SONG WON-OON KIM TAE-HO LEE HIROYUKI KOSHINO ICK-DONG YOO

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.50, no.9, pp.773-777, 1997-09-25 (Released:2006-11-25)

参考文献数

22

被引用文献数

21 35

著者

Ryo Norikoshi Katsunori Kohata Hiroko Shimizu-Yumoto Rie Goto Kazuo Ichimura

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-103, (Released:2016-01-13)

被引用文献数

11

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Petal growth associated with flower opening is due to cell expansion. To elucidate the role of soluble carbohydrates in expansion of petal cells in Eustoma grandiflorum, its soluble carbohydrates were identified, and changes in their subcellular concentrations during flower opening were investigated. In addition to glucose, fructose, sucrose, and myo-inositol, d-bornesitol was identified using 1H-NMR. d-Bornesitol was the major soluble carbohydrate in leaves and stems. Given that cyclitols are known to be the translocated carbohydrates in alfalfa, phloem exudate was analyzed. However, the translocated carbohydrate was suggested to be sucrose, and not d-bornesitol. In the petals, glucose and sucrose content increased whereas d-bornesitol and myo-inositol contents were almost constant during flower opening. The fructose content in petals was very low. Glucose, sucrose, myo-inositol, and d-bornesitol were found mainly in the vacuole, although sucrose was also found in the cytoplasm. In the petals of open flowers, glucose and sucrose concentrations in the vacuole increased to 60 and 53 mM. Inorganic ion concentrations in the symplast and apoplast did not increase during flower opening. The osmotic potential of the symplast and apoplast in the petals was lower at the open stage than the potential of those at the bud stage, and this difference was mainly attributed to increases in glucose and sucrose concentrations. The results suggest that the accumulation of glucose and sucrose in the vacuole reduces the symplastic osmotic potential, which appears to be involved in the cell expansion associated with flower opening, but that the contribution of d-bornesitol as an osmoticum to cell expansion is limited in Eustoma.

著者

Chiang-Soon Song Byung-Yoon Chun Yoo-Im Choi

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.27, no.12, pp.3825-3828, 2015 (Released:2015-12-28)

参考文献数

11

被引用文献数

6

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[Purpose] The purpose of this research was two-fold: (1) to evaluate the effects of father’s parenting participation on parenting stress among mothers with disabled children and (2) more specifically, to investigate the importance of fathers’ parenting participation in decreasing mothers’ parenting stress. [Subjects and Methods] To examine these relationships, this study recruited 82 mothers with disabled children from rehabilitation centers in the Gwangju city. The common characteristics of parents and children, father’s parenting participation, and mother’s parenting stress were assessed using standard questionnaires. [Results] Based on mother reports, findings suggest that as fathers’ parenting participation increases mothers’ parenting stress decreases. [Conclusion] The present study reveals a need for improvement in parenting education and in providing fathers of disabled children with a temporary leave of absence from work. Specifically, this research highlights the importance of shared parenting responsibilities among family members. Finally, our findings suggest that developing a standardized national program for assisting parents with disabled children may help decrease parenting stress in mothers.

著者

保坂 和志 郡司 ペギオ-幸夫

出版者

NTT出版

雑誌

インターコミュニケーション (ISSN:09183841)

巻号頁・発行日

vol.15, no.3, pp.37-61, 2006

著者

edited by Stewart Carter revised and expanded by Jeffery Kite-Powell

出版者

Indiana University Press

巻号頁・発行日

2012

著者

Ho Yu Il Seop Choi Kyung-Lyong Han Jae Yeon Choi Goobong Chung Jinho Suh

出版者

一般社団法人 日本鉄鋼協会

雑誌

ISIJ International (ISSN:09151559)

巻号頁・発行日

vol.55, no.12, pp.2609-2617, 2015-12-15 (Released:2015-12-25)

参考文献数

11

被引用文献数

25

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Handling heavy-load materials is the most common operation in iron and steel making processes. There are numerous operations in which workers directly deal with heavy loads without equipment. The refractory constructions in the converter and AOD (Argon Oxygen Decarburization) furnaces are representative examples. Transferring thousands of heavy materials repeatedly over a long period of time can not only cause musculoskeletal diseases, which occur 70% on the waist and 30% on other parts such as wrists, elbows, shoulders, etc. but also contain latent risks of safety accidents.In this paper, a novel stand-alone powered exoskeleton robot suit was developed for assisting the strength of waist, lower back, and hip joints that are physically vulnerable during handling heavy-load materials. The simple robot structure reduces the frame weight as well enabling easy motion control. The robot is capable of moving freely due to the stand-alone actuators. The developed novel clutch system generates a smooth transition against various working conditions. This technology significantly diminishes the physical fatigue of operators and will subsequently prevent further muscular skeletal disorders as well as safety accidents.

著者

Kosuke Morita Kouji Morimoto Daiya Kaji Takahiro Akiyama Sin-ichi Goto Hiromitsu Haba Eiji Ideguchi Rituparna Kanungo Kenji Katori Hiroyuki Koura Hisaaki Kudo Tetsuya Ohnishi Akira Ozawa Toshimi Suda Keisuke Sueki HuShan Xu Takayuki Yamaguchi Akira Yoneda Atsushi Yoshida YuLiang Zhao

出版者

一般社団法人 日本物理学会

雑誌

Journal of the Physical Society of Japan (ISSN:00319015)

巻号頁・発行日

vol.73, no.10, pp.2593-2596, 2004-10-15 (Released:2007-07-25)

参考文献数

16

被引用文献数

461

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The convincing candidate event of the isotope of the 113th element, 278113, and its daughter nuclei, 274111 and 270Mt, were observed, for the first time, in the 209Bi+70Zn reaction at a beam energy of 349.0 MeV with a total dose of 1.7×1019. Alpha decay energies and decay times of the candidates, 278113, 274111, and 270Mt, were (11.68±0.04 MeV, 0.344 ms), (11.15±0.07 MeV, 9.26 ms), and (10.03±0.07 MeV, 7.16 ms), respectively. The production cross section of the isotope was deduced to be 55+150−45 fb (10−39 cm2).

著者

Ji-young Lee Jun-Gu Oh Jin Sook Kim Kwang-Won Lee

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.37, no.7, pp.1162-1167, 2014-07-01 (Released:2014-07-01)

参考文献数

24

被引用文献数

1 14

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Advanced glycation end-products (AGEs) have been implicated in the development of diabetic complications. We report the antiglycating activity of chebulic acid (CA), isolated from Terminalia chebula on breaking the cross-links of proteins induced by AGEs and inhibiting the formation of AGEs. Aminoguanidine (AG) reduced 50% of glycated bovine serum albumin (BSA) with glycolaldehyde (glycol-BSA)-induced cross-links of collagen at a concentration of 67.8±2.5 mM, the level of CA required for exerting a similar antiglycating activity was 38.8±0.5 µM. Also, the breaking activity on collagen cross-links induced by glycol-BSA was potent with CA (IC50=1.46±0.05 mM), exhibiting 50-fold stronger breaking activity than with ALT-711, a well-known cross-link breaker (IC50=72.2±2.4 mM). IC50 values of DPPH· scavenging activity for CA and ascorbic acid (AA) were 39.2±4.9 and 19.0±1.2 µg dry matter (DM) mL−1, respectively, and ferric reducing and antioxidant power (FRAP) activities for CA and AA were 4.70±0.06 and 11.4±0.1 mmol/FeSO4·7H2O/g DM, respectively. The chelating activities of CA, AG and ALT711 on copper-catalyzed oxidation of AA were compared, and in increasing order, ALT-711 (IC50 of 1.92±0.20 mM)<CA (IC50 of 0.96±0.07 mM)<AG (0.47±0.05 mM). Thus, CA could be a breaker as well as an inhibitor of AGE cross-linking, the activity of which may be explained in large part by its chelating and antioxidant activities, suggesting that CA may constitute a promising antiglycating candidate in intervening AGE-mediated diabetic complications.

著者

Mijung KIM Yongsun KIM Seunghoon LEE Minyoung KUK Ah Young KIM Wanhee KIM Oh-Kyeong KWEON

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0361, (Released:2015-12-27)

被引用文献数

6

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Allogenic adipose-derived mesenchymal stem cells (Ad-MSCs) are an alternative source for cytotherapy owing to their antioxidant and anti-inflammatory effects. Frozen-thawed allogenic Ad-MSCs can be used instantly for this purpose. However, the viability and function of frozen-thawed Ad-MSCs have not been clearly evaluated. The purpose of this study was to compare the viability and function of Ad-MSCs and heme oxygenase-1 (HO-1)-overexpressed Ad-MSCs in vitro after freeze-thawing. The viability, proliferation, antioxidant capacity and mRNA gene expression of growth factors were evaluated. Frozen-thawed cells showed significantly lower viability than fresh cells (77% for Ad-MSCs and 71% for HO-1 Ad-MSCs, P<0.01). However, the proliferation rate of frozen-thawed Ad-MSCs increased and did not differ from that of fresh Ad-MSCs after 3 days of culture. In contrast, the proliferation rate of HO-1-overexpressed Ad-MSCs was lower than that of Ad-MSCs. The mRNA expression levels of TGF-β, HGF and VEGF did not differ between fresh and frozen-thawed Ad-MSCs, but COX-2 and IL-6 had significantly higher mRNA expression in frozen cells than fresh cells (P<0.05). Fresh Ad-MSCs exhibited higher HO-1 mRNA expression than frozen-thawed Ad-MSCs, and fresh HO-1 overexpressed Ad-MSCs exhibited higher than fresh Ad-MSCs (P<0.05). However, there was no significant difference between fresh and frozen HO-1 overexpressed Ad-MSCs. The antioxidant capacity of HO-1-overexpressed Ad-MSCs was significantly higher than that of Ad-MSCs. Cryopreservation of Ad-MSCs negatively affects viability and antioxidant capacity, and HO-1-overexpressed Ad-MSCs might be useful to maximize the effect of Ad-MSCs for cytotherapy.

著者

Lim Sang-min

出版者

九州大学日本語文学会

雑誌

九大日文

巻号頁・発行日

no.11, pp.81-98, 2008-03-31