著者
Yuko Takada-Hoshino Naoyuki Matsumoto
出版者
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
雑誌
Microbes and Environments (ISSN:13426311)
巻号頁・発行日
vol.19, no.1, pp.13-19, 2004 (Released:2004-04-21)
参考文献数
21
被引用文献数
119 121

DNA extraction has been difficult from some types of soil. Seven soil samples from agricultural fields and a forest, which were mainly volcanic ash soils, were used. Soil DNA could be extracted from only two of them using a commercially available kit exploiting bead-beating. When skim milk was added to the extraction buffer at 40 mg g-1 soil, DNA could be detected by electrophoresis from all the samples, indicating that the DNA from lysed cells was adsorbed by soil colloids. The addition of skim milk did not affect PCR-DGGE profiles. The improved method is applicable to the analysis of molecular communities in soils which strongly adsorb DNA.
著者
Takuro NAGAHARA Koichi OHNO Taisuke NAKAGAWA Yuko GOTO-KOSHINO James K CHAMBERS Kazuyuki UCHIDA Naomi MIZUSAWA Chiaki KAGA Maho NAGAZAWA Hirotaka TOMIYASU Hajime TSUJIMOTO
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.22-0172, (Released:2023-01-02)
被引用文献数
3

Intestinal lymphangiectasia (IL) is a common complication in dogs. This study analyzed intestinal microbiota using 16S rRNA amplicon analysis as candidate factors that strongly influence the small intestinal lymphatic vessels in dogs with and without IL. Twelve dogs were included, of which six were diagnosed with lymphoplasmacytic enteritis, four with small-cell lymphoma, and two with large-cell lymphoma. Seven of these dogs had IL, whereas five did not. First, the microbial diversity analyzed by Faith pd index was significantly decreased in dogs with IL compared to dogs without IL. Then, the relative amounts of each bacterial taxa were compared between dogs with and without IL using Linear discriminant analysis effect size analysis. At the genus level, the Ruminococcus gnavus groupsignificantly increased in dogs with IL compared to dogs without IL. A total of four genera, including Ruminococcus torques group and Faecalibacterium, which produce butyrate, significantly decreased in dogs with IL. This study showed decreased intestinal bacterial diversity and several alterations of intestinal microbiota, including a decrease in butyrate-producing bacteria in dogs with IL, compared to dogs without IL.
著者
Jiayu He Yuanyuan Liu Ai Zhang Qianfeng Liu Xueli Yang Naixiu Sun Baoqun Yao Fengchao Liang Xiaochang Yan Yang Liu Hongjun Mao Xi Chen Nai-jun Tang Hua Yan
出版者
The Japanese Society for Hygiene
雑誌
Environmental Health and Preventive Medicine (ISSN:1342078X)
巻号頁・発行日
vol.28, pp.3, 2023 (Released:2023-01-11)
参考文献数
41
被引用文献数
3

Background: Weather conditions are a possible contributing factor to age-related macular degeneration (AMD), a leading cause of irreversible loss of vision. The present study evaluated the joint effects of meteorological factors and fine particulate matter (PM2.5) on AMD.Methods: Data was extracted from a national cross-sectional survey conducted across 10 provinces in rural China. A total of 36,081 participants aged 40 and older were recruited. AMD was diagnosed clinically by slit-lamp ophthalmoscopy, fundus photography, and spectral domain optical coherence tomography (OCT). Meteorological data were calculated by European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis and were matched to participants’ home addresses by latitude and longitude. Participants’ individual PM2.5 exposure concentrations were calculated by a satellite-based model at a 1-km resolution level. Multivariable-adjusted logistic regression models paired with interaction analysis were performed to investigate the joint effects of meteorological factors and PM2.5 on AMD.Results: The prevalence of AMD in the study population was 2.6% (95% CI 2.42–2.76%). The average annual PM2.5 level during the study period was 63.1 ± 15.3 µg/m3. A significant positive association was detected between AMD and PM2.5 level, temperature (T), and relative humidity (RH), in both the independent and the combined effect models. For PM2.5, compared with the lowest quartile, the odds ratios (ORs) with 95% confidence intervals (CIs) across increasing quartiles were 0.828 (0.674,1.018), 1.105 (0.799,1.528), and 2.602 (1.516,4.468). Positive associations were observed between AMD and temperature, with ORs (95% CI) of 1.625 (1.059,2.494), 1.619 (1.026,2.553), and 3.276 (1.841,5.830), across increasing quartiles. In the interaction analysis, the estimated relative excess risk due to interaction (RERI) and the attributable proportion (AP) for combined atmospheric pressure and PM2.5 was 0.864 (0.586,1.141) and 1.180 (0.768,1.592), respectively, indicating a synergistic effect between PM2.5 and atmospheric pressure.Conclusions: This study is among the first to characterize the coordinated effects of meteorological factors and PM2.5 on AMD. The findings warrant further investigation to elucidate the relationship between ambient environment and AMD.
著者
CHANG Yang-Yi
出版者
The Association of Japanese Geographers
雑誌
Geographical review of Japan series B (ISSN:18834396)
巻号頁・発行日
vol.95, no.1, pp.21-36, 2022-06-30 (Released:2022-07-27)
参考文献数
16
被引用文献数
4

This study analyzed the spatial distribution of population and housing in Daejeon Metropolitan City, a regional central city in South Korea, from the perspective of diversity. The diversity index of each administrative Dong is calculated using the population census variables of age, household, and housing type in 2010 and 2015. Variables affecting diversity changes are the increase in the number of people aged 55 or older, the increase in single-person households, and the increase in condominium dwellings. Based on extracted variables, specific population- and housing type- areas of concentration are identified: aged 55 or older, single-person households, detached houses; and age group of 35–44, couples with children, condominium dwellings. The spatial distribution shows that aged 55 or older, single-person households and detached house concentrations are found in the eastern part of the city, mainly the old city area. In contrast, 35–44 years old, couples and children, and condominium concentration districts are found in the western part where the development of condominium complexes occurred. In Daejeon, an elderly population concentration area exists, which is predicted to expand further as the elderly population increases. Since condominium construction serves as a pull factor for 35–44 years old and couples with children, condominium-centered housing construction in old city areas can increase the possibility of preserving age and household diversity.
著者
Noriko Tsubamoto-Sano Junji Ohtani Hiroshi Ueda Masato Kaku Kazuo Tanne Kotaro Tanimoto
出版者
Nihon University School of Dentistry
雑誌
Journal of Oral Science (ISSN:13434934)
巻号頁・発行日
vol.61, no.1, pp.119-124, 2019 (Released:2019-03-28)
参考文献数
34
被引用文献数
4

This study was to investigate the influences of habitual mouth breathing on memory and learning ability during the growth period. At age 5 weeks, the experimental rats were subjected to surgery to close completely one side of the nasal cavity. An 8-arm radial maze was used to evaluate memory and learning ability at age 7, 11, and 15 weeks. Moreover, the brain was extracted at age 7, 11, and 15 weeks, and subjected to histomorphometric examination for the distribution and number of pyramidal cells in the hippocampal CA1 and CA3 regions after Nissl staining. The trial time to accomplish each task was significantly longer in the experimental group than in the control group throughout the experimental period. The number of pyramidal cells was significantly less in the experimental rats than in controls in both the CA1 and CA3 regions for the entire experimental period. Thus, the functional deterioration of the respiratory system during the growth phase exerts a substantial effect on the growth and development of the central nervous system.
著者
Gabriel Alemany-Molina Beatriz Martínez-Sánchez Emilia Morallón Diego Cazorla-Amorós
出版者
The Carbon Society of Japan
雑誌
Carbon Reports (ISSN:24365831)
巻号頁・発行日
vol.1, no.4, pp.162-174, 2022-12-01 (Released:2022-12-01)
参考文献数
93
被引用文献数
2

Engineering the surface chemistry of carbon-based materials is of crucial importance in tuning their intrinsic properties, including electrical conductivity, wettability, electroactivity, adsorption potential, reactivity, physical and chemical stability. Intense research has recently focused on understanding the role of surface oxygen functional groups when carbon materials are in contact with an electrolyte or solvent in order to tailor and improve them for technological applications. For this purpose, their synthesis method and/or further oxidation treatments should be carefully selected, since they can substantially influence not only the oxygen content, but also the nature of the oxygen moieties, which could be decisive in determining the surface properties of the resulting material. The combined use of different chemical, spectroscopic and electrochemical techniques, provides unique and reliable information about the contribution of a specific oxygen-containing group in the surface (electro)chemistry of carbon-based materials. This paper provides a discussion of the role of oxygen heteroatoms in the surface electrochemistry of a carbon material as they relate to their influence on both its electroactivity and reactivity.
著者
Elizabeth OGANDO-RIVAS Paul CASTILLO Jesus Q. BELTRAN Rodolfo ARELLANO Isabel GALVAN-REMIGIO Victor SOTO-ULLOA Roberto DIAZ-PEREGRINO Diana OCHOA-HERNANDEZ Pablo REYES-GONZÁLEZ Elias SAYOUR Duane MITCHELL
出版者
The Japan Neurosurgical Society
雑誌
Neurologia medico-chirurgica (ISSN:04708105)
巻号頁・発行日
pp.2022-0116, (Released:2022-10-25)
参考文献数
93
被引用文献数
2

We understand only a small fraction of the events happening in our brains; therefore, despite all the progress made thus far, a whole array of questions remains. Nonetheless, neurosurgeons invented new tools to circumvent the challenges that had plagued their predecessors. With the manufacturing boom of the 20th century, technological innovations blossomed enabling the neuroscientific community to study and operate upon the living brain in finer detail and with greater precision while avoiding harm to the nervous system. The purpose of this chronological review is to 1) raise awareness among future neurosurgeons about the latest advances in the field, 2) become familiar with innovations such as augmented reality (AR) that should be included in education given their ready applicability in surgical training, and 3) be comfortable with customizing these technologies to real-life cases like in the case of mixed reality.
著者
Anna So Kazuhiko Sakaguchi Yuko Okada Yasuko Morita Tomoko Yamada Hiroshi Miura Natsu Otowa-Suematsu Tomoaki Nakamura Hisako Komada Yushi Hirota Yoshikazu Tamori Wataru Ogawa
出版者
The Japan Endocrine Society
雑誌
Endocrine Journal (ISSN:09188959)
巻号頁・発行日
vol.67, no.5, pp.501-507, 2020 (Released:2020-05-28)
参考文献数
30
被引用文献数
11 21

We had aimed to determine whether homeostasis model assessment–insulin resistance (HOMA-IR) reflects insulin resistance-sensitivity during treatment with a sodium-glucose cotransporter 2 inhibitor (SGLT2i). Hyperinsulinemic-euglycemic clamp analysis was performed in 22 patients with type 2 diabetic patients taking dapagliflozin (5 mg/day before or after breakfast). Propensity score matching of these individuals (SGLT2i group) for age, sex, body mass index, and clamp-derived tissue glucose uptake rate with 44 type 2 diabetic patients who had undergone clamp analysis without SGLT2i treatment (control group) identified 17 paired subjects in each group for further analysis of the relation between HOMA-IR and a clamp-derived insulin sensitivity index (ISI). Natural log–transformed HOMA-IR was negatively correlated with ISI in both SGLT2i (r = –0.527, p = 0.030) and control (r = –0.534, p = 0.027) groups. The simple regression lines for log-transformed HOMA-IR and ISI in the two groups showed similar slopes but differed in their intercepts. Multivariate analysis revealed that HOMA-IR for patients with the same ISI in the two groups was related by the formula: HOMA-IRcontrol = HOMA-IRSGLT2i × 2.45. In conclusion, HOMA-IR was well correlated with ISI during SGLT2i treatment, but values corresponding to the same ISI were lower in the SGLT2i group than in the control group.
著者
Charley-Lea POLLARD Zamira GIBB Aleona SWEGEN Christopher G. GRUPEN
出版者
The Society for Reproduction and Development
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2022-052, (Released:2022-09-27)
被引用文献数
6

Oocyte quality is the limiting factor in female fertility. It is well known that maternal nutrition plays a role in reproductive function, and manipulating nutrition to improve fertility in livestock has been common practice in the past, particularly with respect to negative energy balance in cattle. A deficiency in nicotinamide adenine dinucleotide (NAD+) production has been associated with increased incidences of miscarriage and congenital defects in humans and mice, while elevating NAD+ through dietary supplements in aged subjects improved oocyte quality and embryo development. NAD+ is consumed by Sirtuins and poly-ADP-ribose polymerases (PARPs) within the cell and thus need constant replenishment in order to maintain various cellular functions. Sirtuins and PARPs play important roles in oocyte maturation and embryo development, and their activation may prove beneficial to in vitro embryo production and livestock breeding programs. This review examines the roles of NAD+, Sirtuins and PARPs in aspects of fertility, providing insights into the potential use of NAD+-elevating treatments in livestock breeding and embryo production programs.
著者
Hisashi Naito Toshinori Yoshihara Ryo Kakigi Noriko Ichinoseki-Sekine Takamasa Tsuzuki
出版者
The Japanese Society of Physical Fitness and Sports Medicine
雑誌
The Journal of Physical Fitness and Sports Medicine (ISSN:21868131)
巻号頁・発行日
vol.1, no.1, pp.125-131, 2012-05-25 (Released:2012-08-22)
参考文献数
61
被引用文献数
9 10

Many researchers have been interested in the effects of heat stress on skeletal muscle. Recently, it has been demonstrated that heat stress on skeletal muscle results in an increase in muscle protein mass and subsequent muscle hypertrophy, and attenuation of muscle atrophy. Although the cellular mechanism remains unclear, it is thought that heat shock proteins (HSPs), which are highly conserved proteins induced by heat stress, play a major role in these phenomena. However, new insights suggest that heat stress-induced muscle hypertrophy and the prevention of muscle atrophy may be regulated not only by elevated HSP expression but also by multiple signaling pathways associated with protein synthesis and breakdown. Additionally, heat stress seems to cause various changes in other muscle functions. Although further studies are required to reveal the molecular biological mechanisms involved in the heat stress-induced changes in skeletal muscle, heat stress may be a useful tool for increasing muscle mass, attenuating disuse skeletal muscle atrophy, facilitating an early recovery from muscle damage, and improving glucose metabolism. This paper reviews studies of these effects of heat stress on skeletal muscle.
著者
Jae-yong Lee Seung-Min Lee Seung-Jae Lee
出版者
公益社団法人 日本気象学会
雑誌
SOLA (ISSN:13496476)
巻号頁・発行日
vol.18, pp.173-180, 2022 (Released:2022-08-31)
参考文献数
33
被引用文献数
1

This study performed 4-day numerical integration in 1-hour intervals using the Weather Research and Forecasting (WRF) model for four major cases of heavy snowfall that occurred from 2020 to 2021. The model-predicted snow depth data were compared with the ground-observed snow depth and the satellite-observed snow cover data and then were statistically verified. The scalar verification results for ground data from the four cases showed a root–mean–square error of 2.55-16.67 cm and a correlation coefficient of 0.48-0.80, whereas the verification results with satellite data showed the correlation coefficients of 0.38-0.60. For categorical verification, using a threshold value of a snow depth exceeding 5 cm, the proportion correct was 90% or higher for ground observations of each case. In addition, in the satellite categorical verification, when the threshold value of the Snow Cover Fraction (SCF) exceeds 0.5, the proportion correct was 50% or more. These results are meaningful because the model snow depth verification methods were devised strategically for the first time using both the snow depth data of the mesoscale ground observation networks and ultra-high-resolution Sentinel-2 satellite data currently available in Korea. The findings of this study will contribute to the development of a high-resolution numerical prediction model and its verification methodology for snowfalls in the Korean Peninsula, eventually leading to increased prediction accuracy and reduced snow damage.
著者
Chien-An Yao I-Ling Chen Chung-Yen Chen Pao-Ling Torng Ta-Chen Su
出版者
Japan Atherosclerosis Society
雑誌
Journal of Atherosclerosis and Thrombosis (ISSN:13403478)
巻号頁・発行日
pp.63254, (Released:2022-04-20)
参考文献数
46
被引用文献数
1

Aim: This study aimed to determine whether sleep disturbance, defined as the wakeup frequency at night, is associated with atherogenic dyslipidemia and to explore possible sex differences. Methods: A total of 1,368 adults aged 19–70 years were included in the study of lifestyles and atherogenic dyslipidemia at the National Taiwan University Hospital in the period of 2008–2012. They completed a questionnaire regarding lifestyle information and sleep quality, including sleep hour duration, use of sleeping pills, and wakeup frequency during nighttime sleep. The measured lipid profiles included total cholesterol, triglycerides, low- and high-density lipoprotein cholesterol (LDL-C and HDL-C, respectively), non-HDL-C, and small dense LDL-C (sdLDL-C). Multivariate logistic regression was performed to determine habitual interrupted sleep and the odds ratio of atherogenic dyslipidemia following adjustment for conventional risk factors and for sex-based subgroup analysis. Results: A wakeup frequency ≥ 3 times per night was independently associated with an increased risk [odds ratio (95% confidence interval)] of dyslipidemia was 1.96 (1.17–3.28), and non-HDL-C ≥ 160 mg/dL was 1.78 (1.09–2.89). A higher wakeup frequency was associated with increased atherogenic dyslipidemia in women than in men. The multivariate adjusted relative risks for non-HDL ≥ 160 mg/dL and cholesterol ≥ 200 mg/dL were 3.05 (1.27–7.34) and 4.01(1.29-12.45) for female individuals with insomnia and those with a wakeup frequency ≥ 2 times per night, respectively. Conclusion: A higher wakeup frequency was associated with atherogenic dyslipidemia in Taiwanese adults, particularly in women. This study also provided another evidence of increasing cardiovascular diseases in subjects with habitual interrupted sleep.
著者
Jae-yong Lee Seung-Min Lee Seung-Jae Lee
出版者
公益社団法人 日本気象学会
雑誌
SOLA (ISSN:13496476)
巻号頁・発行日
pp.2022-028, (Released:2022-07-19)
被引用文献数
1

This study performed 4-day numerical integration in 1-hour intervals using the Weather Research and Forecasting (WRF) model for four major cases of heavy snowfall that occurred from 2020 to 2021. The model-predicted snow depth data were compared with the ground-observed snow depth and the satellite-observed snow cover data and then were statistically verified. The scalar verification results for ground data from the four cases showed a root–mean–square error of 2.55-16.67 cm and a correlation coefficient of 0.48-0.80, whereas the verification results with satellite data showed the correlation coefficients of 0.38-0.60. For categorical verification, using a threshold value of a snow depth exceeding 5 cm, the proportion correct was 90% or higher for ground observations of each case. In addition, in the satellite categorical verification, when the threshold value of the Snow Cover Fraction (SCF) exceeds 0.5, the proportion correct was 50% or more. These results are meaningful because the model snow depth verification methods were devised strategically for the first time using both the snow depth data of the mesoscale ground observation networks and ultra-high-resolution Sentinel-2 satellite data currently available in Korea. The findings of this study will contribute to the development of a high-resolution numerical prediction model and its verification methodology for snowfalls in the Korean Peninsula, eventually leading to increased prediction accuracy and reduced snow damage.
著者
Maria Teresa B Abola Jonathan Golledge Tetsuro Miyata Seung-Woon Rha Bryan P Yan Timothy C Dy Marie Simonette V Ganzon Pankaj Kumar Handa Salim Harris Jiang Zhisheng Ramakrishna Pinjala Peter Ashley Robless Hiroyoshi Yokoi Elaine B Alajar April Ann Bermudez-delos Santos Elmer Jasper B Llanes Gay Marjorie Obrado-Nabablit Noemi S Pestaño Felix Eduardo Punzalan Bernadette Tumanan-Mendoza
出版者
Japan Atherosclerosis Society
雑誌
Journal of Atherosclerosis and Thrombosis (ISSN:13403478)
巻号頁・発行日
vol.27, no.8, pp.809-907, 2020-08-01 (Released:2020-08-01)
被引用文献数
1 49

Background: Peripheral artery disease (PAD) is the most underdiagnosed, underestimated and undertreated of the atherosclerotic vascular diseases despite its poor prognosis. There may be racial or contextual differences in the Asia-Pacific region as to epidemiology, availability of diagnostic and therapeutic modalities, and even patient treatment response. The Asian Pacific Society of Atherosclerosis and Vascular Diseases (APSAVD) thus coordinated the development of an Asia-Pacific Consensus Statement (APCS) on the Management of PAD. Objectives: The APSAVD aimed to accomplish the following: 1) determine the applicability of the 2016 AHA/ACC guidelines on the Management of Patients with Lower Extremity Peripheral Artery Disease to the Asia-Pacific region; 2) review Asia-Pacific literature; and 3) increase the awareness of PAD. Methodology: A Steering Committee was organized to oversee development of the APCS, appoint a Technical Working Group (TWG) and Consensus Panel (CP). The TWG appraised the relevance of the 2016 AHA/ACC PAD Guideline and proposed recommendations which were reviewed by the CP using a modified Delphi technique. Results: A total of 91 recommendations were generated covering history and physical examination, diagnosis, and treatment of PAD—3 new recommendations, 31 adaptations and 57 adopted statements. This Asia-Pacific Consensus Statement on the Management of PAD constitutes the first for the Asia-Pacific Region. It is intended for use by health practitioners involved in preventing, diagnosing and treating patients with PAD and ultimately the patients and their families themselves.
著者
Mauricio Hernández Sergio Ancona Aníbal H. Díaz De La Vega-Pérez Ligia C. Muñoz-Arenas Stephanie E. Hereira-Pacheco Yendi E. Navarro-Noya
出版者
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
雑誌
Microbes and Environments (ISSN:13426311)
巻号頁・発行日
vol.37, no.3, pp.ME21087, 2022 (Released:2022-06-30)
参考文献数
66

The gut microbiota influences the phenotype and fitness of a host; however, limited information is currently available on the diversity and functions of the gut microbiota in wild animals. Therefore, we herein examined the diversity, composition, and potential functions of the gut microbiota in three Sceloporus lizards: Sceloporus aeneus, S. bicanthalis, and S. grammicus, inhabiting different habitats in a mountainous ecosystem. The gut bacterial community of S. bicanthalis from alpine grasslands at 4,150‍ ‍m a.s.l. exhibited greater taxonomic, phylogenetic, and functional alpha diversities than its sister species S. aeneus from cornfields and human-induced grasslands at 2,600‍ ‍m‍ ‍a.s.l. Bacteria of the genus Blautia and metabolic functions related to the degradation of aromatic compounds were more abundant in S. bicanthalis than in S. aeneus, whereas Oscillibacter and predicted functions related to amino acid metabolism and fermentation were more abundant in S. aeneus. The structure of the dominant and most prevalent bacteria, i.e., the core microbiota, was similar between the sister species from different habitats, but differed between S. grammicus and S. aeneus cohabiting at 2,600‍ ‍m‍ ‍a.s.l. and between S. grammicus and S. bicanthalis cohabiting at 4,150‍ ‍m a.s.l. These results suggest that phylogenetic relatedness defines the core microbiota, while the transient, i.e., non-core, microbiota is influenced by environmental differences in the habitats. Our comparisons between phylogenetically close species provide further evidence for the specialized and complex associations between hosts and the gut microbiota as well as insights into the roles of phylogeny and ecological factors as drivers of the gut microbiota in wild vertebrates.