著者

Junji MAGAE Kazuo NAGAI Kunio ANDO Gakuzo TAMURA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.12, pp.3143-3147, 1988 (Released:2006-04-05)

参考文献数

12

被引用文献数

9 1

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Mouse myeloid leukemia cells, Ml, were induced to differentiate into phagocytes by treatment with ascofuranone (AF). AF also induced differentiation of human promyelocytic leukemia HL60 cells and human erythroid leukemia K562 cells into granulocytes and erythrocytes, as detected by nitroblue tetrazolium reducing activity and benzidine staining, respectively. The antibiotic enhanced acetate incorporation of K562 cells. The increase was not observed with the cells of HL60 and two human B lymphoma lines, Daudi and Raji. The increase was diminished by the addition of a glycolysis inhibitor, deoxyglucose. Inhibitors of respiration, antimycin and sodium azide, also enhanced acetate incorporation of K562 cells specifically, which was diminished by the addition of deoxyglucose. Furthermore, antimycin induced differentiation of K562 and HL60 cells. These results suggest a possible relationship between cell differentiation and inhibition of respiration.

著者

時友 裕紀子 小林 彰夫

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.62, no.1, pp.17-22, 1988

被引用文献数

1 3

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(1) アズキを煮熟した際感じられる独特の甘く香ばしいにおいについて検討する目的で,北海道十勝産のアズキのにおい濃縮物を連続水蒸気蒸留抽出法にて得,シリカゲルカラムクロマトグラフィー, GLCおよびGC-MS分析により,各種におい成分を検討した.<br> (2) におい濃縮物は甘く香ばしいアズキらしいにおいを有しており, 0.8~0.9mg%の収率であった.音更シュウズ,ハヤテショウズの2品種のにおい成分を比較したが,においの構成成分およびその量比に顕著な違いは認められなかった. 2品種のにおい濃縮物,および音更ショウズのにおい濃縮物のシリカゲルカラムクロマトグラフィーによって得られた各画分を分析した結果,あわせて70化合物を同定および推定した.それらは,炭化水素14種,アルコール22種,カルボニル化合物15種,カルボン酸2種,フェノール7種,フラン5種,ラクトン2種,含窒素化合物3種であった.<br> (3) 上記のにおい物質のうち,豆臭を有する1-hexanol, 3-methyl-1-butanolなどのアルカノール,甘いにおいに寄与すると考えられる芳香族アルコール,フラン,ラクトン,甘い香気を増強すると考えられるフェノール化合物,および,含有量が多くカラメル様芳香を有するmaltolが,アズキの煮熟臭成分として重要であると考えられた.とくに,アズキの特徴的な甘いにおいには,maltolが大きく関与していることが明らかとなった.

著者

西田 孝太郎

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.10, no.4, pp.374-378, 1934

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前に記載した實驗結果はこれを次の如く要約することが出來る.<br> (1) 蘇鐵種子の重量はその成熟中或時期までは増加すれども以後減少する,而して種子を胚乳部と種皮部との部分別として考へても何れも同樣の傾向を示すのである.<br> (2) 胚乳中の水分は漸次減少したる後増加する,然るに種皮中の水分は漸次減少する一方である.<br> 以下胚乳部の成分について述ぶれば;<br> (3) 灰分,純蛋白,澱粉,糊精及び可溶無窒素物の含量は水分量と正反對に次第に増加し一定時期に最大となり,後再び減少するのであつて,此等の諸成分は乾物量と全く平行する傾向を示して居る.<br> (4) 糖類は成熟中漸次増加する傾向を示すのであるが,これと全く反對に非蛋白窒素化合物及び粗纖維の量は明かに次第に減ずる.<br> (5) 脂肪の含量は次第に減少したる後再び増加する傾向を示すに反し,粗蛋白は一時増加したる後減少する.<br> (6) 8月30日採取の試料は著しく未熟で水分含量が極めて大であるが,各成分を乾物量に改算する時特に甚しく目立つのは,非蛋白窒素,還元糖及び灰分の割合が極めて大いことゝ澱粉の割合が少いことである.蓋しこれは蛋白及び澱粉合成の初期にある種子として當然のことであらう.<br> (7) 澱粉原料としての蘇鐵種子收穫の適期は,第2表から判る樣に澱粉含量の最も多い11月下旬と云ふことが出來る.然し第1表に示す通り胚乳の重量の關係上,種子1個中の澱粉の絶對量は第3表の通りに10月下旬採取のものが最も多い結果になつて居る.然し乍其差は僅少であるから結局蘇鐵種子收穫の適期は10月下旬に初まり11月までゞあると云ふことが出來ると思ふ.

著者

Kenshiro FUJIMOTO Hiroko OHMURA Takashi KANEDA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.50, no.1, pp.101-108, 1986 (Released:2006-04-05)

参考文献数

34

被引用文献数

2

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The biological antioxidant activities of bromophenols (5-bromo-3, 4-dihydroxy benzaldehyde and 5-bromo-3, 4-dihydroxy benzylalcohol), protocatechualdehyde and vanillyl octadecanamide were compared with α-tocopherol in vitro and in vivo. All of these antioxidants significantly inhibited the lipid peroxidation in microsomal suspensions induced by ascorbate/Fe(II) or NADPH. By removing the non-binding free antioxidants from the suspensions, the inhibitory activities markedly decreased in antioxidants other than α-tocopherol; however, considerable activity was still observed. The effects of orally administered antioxidants on the symptoms of vitamin E deficiency were examined using rats fed with a vitamin E-free diet and supplemented with oxidized oils. As a result and contrary to the effects in vitro, all of the antioxidants were much less effective in liver. However, some of the vitamin E-deficient symptoms such as organic solvent-soluble fluorescent materials in the heart and lung, and oxidative hemolysis were significantly suppressed by some antioxidants.

著者

根岸 孝 伊藤 精亮 藤野 安彦

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.45, no.8, pp.374-377, 1971

被引用文献数

1

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スフィゴシルボスホリルコリンが<i>Cl. perjringens</i>から調製したホスホリパーゼCによってスフィンゴシンとリン酸コリンに分解されることを証明した.この場合,エリスロースフィンゴシルホスホリルコリンの方が,スレオースフィンゴシルホスホリルコリンよりも分解されやすいことを認めた.<br> 本研究は,主として文部省科学研究費によって行なわれた.記して謝意を表する.

著者

古沢 良雄 黒沢 雄一郎 中馬 一操

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.47, no.6, pp.359-365, 1973

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市販和漢薬用植物63種について,カゼインを基質としたときの抗トリプシン作用を検討した.その結果, 50%以上の阻害率を示したものは桂皮,牡丹,黄柏,杏仁,甘草,沢潟,ヒシの実(果皮),土茯苓,麻黄,枸杞(茎部),括楼根,白扁豆の12種であった.このうち,黄柏,甘草,沢潟,枸杞,括楼根,白扁豆の阻害物質は,非透析性を示した.<br> Fig. 8. Inhibitory Effects of HTI and Soy Bean Trypsin Inhibitor (STI) on Several Proteinases.<br> Table IV. Anti-inflammatory Effect of HTI on Carrageenin Indaced Edema of Rat Hind Paw<br> (1) 桂皮の阻害物質は,皮粉処理により完全に吸着されることや,種々の呈色反応から,カテコールタンニンか,それに類似した物質であろうと推定した.<br> (2) 甘草の成分グリチルリチンおよび,その加水分解物であるグリチルレチン酸の抗トリプシン作用を検討した.グリチルレチン酸は,グリチルリチンの10数倍の抗トリプシン作用を示し,さらに抗キモトリプシン作用もみられたが,植物性および細菌起源の蛋白分解酵素に対する阻害作用は,認められなかった.<br> (3) ヒシの実の阻害物質は,局方タンニン酸と一致した.<br> (4) 白扁豆の阻害物質は温水で抽出され,アセトンで粗末として沈殿分離された.粗末はトリプシン,キモトリプシンに対して阻害作用を示したが,植物性のブロメラインや細菌起源の蛋白分解酵素に対しては阻害しなかった.また,粗末は酸やペプシンに安定であったが,アルカリでは不安定であった.さらに,カラゲニン浮腫に対し,皮下投与で有意な抑制を示した.しかし,経口投与では対照と有意な差はみられなかった.

著者

廣海 啓太郎 森田 雄平

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.22, no.9, pp.552-559, 1984

被引用文献数

1 1

著者

Ken SASAKI Hiroyo MORIKAWA Takashi KISHIBE Ayaka MOKAMI Toshihiko HARADA Masahiro OHTA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

pp.1203292864, (Released:2012-04-07)

参考文献数

15

被引用文献数

16

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About 90% of the radioactive Cs in the sediment mud of a school’s swimming pool in Fukushima, Japan was removed by treatment for 3 d using the alginate immobilized photosynthetic bacterium Rhodobcater sphaeroides SSI. Even though batch treatment was carried out 3 times repeatedly, the activity of immobilized cells in removing Cs was maintained at levels of about 84% (second batch) and 78% (third batch). Cs was strongly attached to the sediment mud because, even with HNO3 treatment at pH of 2.00–1.60 for 24 h, it was not eluted into the water. Furthermore, more than 75% of the Cs could be removed without solubilization with HNO3. This suggests that the Cs attached to the sediment mud was transformed into immobilized cells via the Cs+ ion by the negative charge of the immobilized cell surface and/or the potassium transport system of the photosynthetic bacterium.

著者

Haruya TAKAHASHI Young-Il KIM Shizuka HIRAI Tsuyoshi GOTO Chie OHYANE Taneaki TSUGANE Chiaki KONISHI Takashi FUJII Shuji INAI Yoko IIJIMA Koh AOKI Daisuke SHIBATA Nobuyuki TAKAHASHI Teruo KAWADA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.8, pp.1621-1624, 2011-08-23 (Released:2011-08-23)

参考文献数

14

被引用文献数

16

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Peroxisome proliferator-activated receptor-α (PPARα) regulates lipid metabolism. We have reported that tomato fruit contains 9-Oxo-(10E,12E)-octadecadienoic acid (9-Oxo-(10E,12E)-ODA), a PPARα agonist. In this study, we found that various tomato samples contained 9-Oxo-(10E,12Z)-ODA and its 13-Oxo-ODA isomers. Furthermore, several isomers showed structural stability under hot and acidic conditions.

著者

Ulala FUNADA Masahiro WADA Tetsunori KAWATA Nobuo TANAKA Tadahiro TADOKORO Akio MAEKAWA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.64, no.10, pp.2053-2058, 2000 (Released:2005-02-03)

参考文献数

36

被引用文献数

3

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Cyanocobalamin, deoxyadenosylcobalamin and methylcobalamin were separately administered to mice sensitized with the ovalbumin antigen to investigate the allergic response. The serum IgE and pulmonary histamine concentrations were significantly lower in all cobalamin (Cbl)-administered groups. The production of interleukin (IL)-2 and IL-4 in splenocytes was also lower in all Cbl-administered groups. These results show that Cbls were effective in mitigating allergic reactions and IL-2 production. CD3+CD28+ (CD28 is an accessory molecule related to IL-2 production) and CD4+CD28+ in splenocytes were higher in all the Cbl-administered groups. However, CD3+CD28-, CD4+CD28- and CD5+CD25- (CD25: IL-2 Rα/p55) were lower in the Cbl-administered groups. In addition, Cbl specifically inhibited the cellular phosphorylation of tyrosine induced by ovalbumin sensitization. These results indicate that the signal in a cell by CD 28 was restrained by Cbl. We infer that Cbl administration significantly reduced the IL-2 concentration, and secondarily the IL-4, IgE and histamine concentrations.

著者

Ryohei KONO Yoshiharu OKUNO Ken-ichi INADA Akihiko TOKUDA Hiroshi HASHIZUME Munehito YOSHIDA Misa NAKAMURA Hirotoshi UTSUNOMIYA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.10, pp.1907-1911, 2011-10-23 (Released:2011-10-23)

参考文献数

35

被引用文献数

19

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Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

著者

Jun TAKEBAYASHI Yasuyuki YAGI Rie ISHII Shigeki ABE Kazuhiko YAMADA Akihiro TAI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.72, no.6, pp.1558-1563, 2008-06-23 (Released:2008-06-23)

参考文献数

25

被引用文献数

20

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The antioxidant activity of a provitamin C agent, 2-O-β-D-glucopyranosyl-L-ascorbic acid (AA-2βG), was compared to that of 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) and ascorbic acid (AA) using four in vitro methods, 1,1-diphenyl-picrylhydrazyl (DPPH) radical-scavenging assay, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS•+)-scavenging assay, oxygen radical absorbance capacity (ORAC) assay, and 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis inhibition assay. AA-2βG slowly and continuously scavenged DPPH radicals and ABTS•+ in roughly the same reaction profiles as AA-2G, whereas AA quenched these radicals immediately. In the ORAC assay and the hemolysis inhibition assay, AA-2βG showed similar overall activities to AA-2G and to AA, although the reactivity of AA-2βG against the peroxyl radical generated in both assays was lower than that of AA-2G and AA. These data indicate that AA-2βG had roughly the same radical-scavenging properties as AA-2G, and a comprehensive in vitro antioxidant activity of AA-2βG appeared to be comparable not only to that of AA-2G but also to that of AA.

著者

小島 正秋 外山 信男 藤井 昇 橋爪 昭人 三浦 道雄 日高 敏郎 玉井 理 駒形 和男

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.61, no.10, pp.1341-1345, 1987

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8回にわたる高農めぐりも今回で終りを迎えます.前回の千葉高等園芸の場合と同じく,座談会に出席の先生は,高農から新制大学の創立時に入学され,母校に教鞭をとっておられる方々がほとんどでありました.<br> 和気あいあいに想い出がそのまま高農から宮崎大学農学部への歴史を追うことになりました.司会の駒形先生もにれが最後の御気持からか,大変リラックスされて,従来とはやや異なる内容となりました.<br> 座談会のために奔走してくださった宮崎大学の三浦道雄先生をはじめ御協力いただいた先生方に心から御礼申し上げます.(編集部)

著者

Taiki MIYAZAWA Kiyotaka NAKAGAWA Fumiko KIMURA Akira SATOH Teruo MIYAZAWA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.9, pp.1856-1858, 2011-09-23 (Released:2011-09-23)

参考文献数

15

被引用文献数

26

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A randomized, double-blind human trial was conducted to assess the effect on the plasma carotenoid concentration of 4- or 12-week astaxanthin supplementation (1 or 3 mg/d) of 20 Japanese middle-aged and senior subjects. The plasma carotenoid concentration was significantly higher after the astaxanthin supplementation than that before in both the 1 mg/d (10 subjects) and 3 mg/d (10 subjects) groups.

著者

Tetsuya ISAKA Morifumi HASEGAWA Hiroaki TOSHIMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

pp.1109292684, (Released:2011-11-07)

参考文献数

28

被引用文献数

20

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Cyclization of the synthesized epoxide precursors of indole mono-, sesqui- and diterpene alkaloids was performed to elucidate the mechanism for biomimetic cationic cyclization to polycyclic structures. 3-(6,7-Epoxygeranyl)indole (11), 3-(10,11-epoxyfarnesyl)indole (2) and 3-(14,15-epoxygeranylgeranyl)indole (3) were respectively synthesized from geraniol, farnesol and geranylgeraniol in 6 or 7 steps. Four Lewis acids (MeAlCl2, BF3·OEt2, TiCl4 and SnCl4) were applied for biomimetic cyclization of the synthesized epoxide precursors. The cyclization products (one product from 11, four products from 2, and three products from 3) were isolated after separation by chromatography. Their structures were determined by using NMR (COSY, HSQC, HMBC, NOESY, etc.) and HRMS analyses. The results show that biomimetic cyclization gave new polycyclic compounds similar to natural indole terpene alkaloids. We conclude that the stability of cation intermediates should determine the preference for product formation by biomimetic cyclization when using a Lewis acid.

著者

Yasunori MAEJIMA Hiroki NAKATSUGAWA Daiki ICHIDA Mayumi MAEJIMA Yasuo AOYAGI Takashi MAOKA Hideo ETOH

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.9, pp.1708-1712, 2011-09-23 (Released:2011-09-23)

参考文献数

25

被引用文献数

18

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Fermented buckwheat sprouts (FBS) are used as multifunctional foods. Their production process includes fermentation with lactic acid bacteria. The major strains were found to include Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus pentosus, Lactococcus lactis subsp. lactis, and Pediococcus pentosaceus in an investigation of the lactic acid bacteria. We searched for the functional components, and nicotianamine (NA) and 2″-hydroxynicotianamine (HNA) were identified as angiotensin I-converting enzyme (ACE) inhibitors. NA and HNA increased during fermentation. Indole-3-ethanol was identified as an antioxidant (a SOD active substance), and may have been generated from tryptophan during fermentation because it was not contained in green buckwheat juice. A safety test demonstrated that FBS contained were safe functional food components, showing negative results in buckwheat allergy tests. Any buckwheat allergy substances might have been degraded during the fermentation process.

著者

Natsu TANIKAWA Kumi YOSHIDA Tadao KONDO Takayuki MIZUNO Tsukasa IWASHINA Masayoshi NAKAYAMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

pp.1109052652, (Released:2011-10-07)

参考文献数

8

被引用文献数

5

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A new tetraglycosyl flavonol, 3-O-[2-O-xylosyl-6-O-(3-O-glucosyl-rhamnosyl) glucosyl] kaempferol was isolated from pale purplish-pink petals of Wabisuke camellia cv. Tarokaja with three known flavonols. It was named urakunoside after the species name of Tarokaja, Camellia uraku. Urakunoside was a major flavonol component in the Tarokaja petals, but was not detected in petals of Tarokaja’s presumed ancestor species.

著者

Tomoko YAMAMOTO Shigeru SUGIYAMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.74, no.2, pp.408-410, 2010-02-23 (Released:2010-02-23)

参考文献数

6

被引用文献数

3

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A bow with horse tail hair is used to play the violin. New and worn-out bow hairs were observed by atomic force microscopy. The cuticles of the new bow hair were already damaged by bleach and delipidation, however the worn-out bow hairs were much more damaged and broken off by force, which relates to wearing out.

著者

都甲 潔

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.46, no.12, pp.865-871, 2008-12-01

著者

SHIOZAKI Shozo SHIMIZU Sakayu YAMADA Hideaki

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.48, no.9, pp.2293-2300, 1984

被引用文献数

11 40

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In survey studies on microbial accumulation of S-adenosyl-L-methionine (AdoMet), various yeasts were found to accumulate AdoMet intracellularly to a high concentration when they were grown in medium containing L-methionine. A group of sake yeasts (<i>Saccharomyces sake</i>) exhibited especially high accumulation. Of these yeasts, <i>S. sake</i> Kyokai No. 6 (K-6), which exhibited the highest accumulation, produced 12.6 μmol (5.03 mg) of AdoMet/ml broth. Almost all AdoMet produced was accumulated in cells, extracellular accumulation of AdoMet being very low. The maximum content of AdoMet of cells was 5.31 μmol (205 mg)/g dry cells. This was the highest value that had been reported. Methionine adenosyltransferase (EC 2.5.1.6) activity was significantly higher in this yeast compared to those in other yeasts tested. Ultraviolet photomicrographic studies on <i>S. sake</i> K-6 suggested that AdoMet was gradually accumulated in vacuoles with the passage of cultivation time.