著者

Matsumoto Yuki Murakami Yoshie Tsuru Saburo Ying Bei-Wen Yomo Tetsuya

出版者

BioMed Central

雑誌

BMC genomics (ISSN:14712164)

巻号頁・発行日

vol.14, pp.808, 2013-11

被引用文献数

41 2

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BackgroundCell growth rate reflects an organism’s physiological state and largely relies on the ability of gene expression to respond to the environment. The relationship between cellular growth rate and gene expression remains unknown.ResultsGrowth rate-coordinated changes in gene expression were discovered by analyzing exponentially growing Escherichia coli cells cultured under multiple defined environments, in which osmotic pressure, temperature and starvation status were varied. Gene expression analyses showed that all 3,740 genes in the genome could be simply divided into three clusters (C1, C2 and C3), which were accompanied by a generic trend in the growth rate that was coordinated with transcriptional changes. The direction of transcriptional change in C1 indicated environmental specificity, whereas those in C2 and C3 were correlated negatively and positively with growth rates, respectively. The three clusters exhibited differentiated gene functions and gene regulation task division.ConclusionsWe identified three gene clusters, exhibiting differential gene functions and distinct directions in their correlations with growth rates. Reverses in the direction of the growth rate correlated transcriptional changes and the distinguished duties of the three clusters indicated how transcriptome homeostasis is maintained to balance the total expression cost for sustaining life in new habitats.

著者

Yae Matsuo Marcus Sandri Norman Mangner Nicolas Majunke Ingo Dähnert Gerhard Schuler Masahiko Kurabayashi Sven Möbius-Winkler

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-13-0828, (Released:2014-01-11)

参考文献数

29

被引用文献数

7 17

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Background: Transcatheter left atrial appendage (LAA) closure is an alternative therapy for stroke prevention in atrial fibrillation (AF) patients. However; real-world efficacy, safety and complications have yet to be investigated. We sought to determine the procedural outcomes and potential complications of LAA closure in routine clinical practice at a high-volume center. Methods and Results: The study group comprised 179 patients (105 males; 72.7±9.0 years) with AF undergoing LAA closure at a single center in Germany. The rate of successful implantation was 98.9% (2 patients did not undergo implantation) and the overall procedure-related complication rate was 11.2% (major: 3.3%: tamponade 2; possibility of transient ischemic attack (TIA) 1; device dislocation 3; minor: 7.8%: pericardial effusion 2; air embolization with transient ST segment elevation 3; thrombus on device/sheath 3; puncture complications 5). At 45 days; 99.4% showed successful sealing of the LAA and 94.5% discontinued oral anticoagulation (OAC). TIA occurred in 2 patients during 6-month follow-up; but no cases of stroke were reported. There were no hemorrhagic stroke or device-related deaths. Only 1 patient was hospitalized with traumatic subdural hematoma. Minor bleeding was reported in 5 patients. Conclusions: Transcatheter LAA closure in a high-volume center is safe and feasible. Life-threatening complications are rare. Discontinuation of OAC 45 days after implantation was also safe.

著者

Joong-Chul Lee Ji Youn Kim Gi Duck Park

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.25, no.12, pp.1595-1599, 2013 (Released:2014-01-08)

参考文献数

8

被引用文献数

2 5

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[Purpose] To examine changes in the knee joint’s isokinetic muscle functions following systematic and gradual rehabilitation exercises lasting for 12 weeks for male and female patients who underwent anterior cruciate ligament (ACL) reconstruction. Differences in muscle functions between the uninvolved side (US) and the involved side (IS) before surgery, differences in muscle functions between US and IS after rehabilitation exercises lasting for 12 weeks, and changes in muscle functions on US and IS between before and after surgery were analyzed to examine the effects of accelerated rehabilitation exercises after ACL reconstruction. [Subjects] The study subjects were 10 patients, five females and five males, who underwent ACL reconstruction performed by the same surgeon. [Methods] As a measuring tool, a Biodex Multi-joint system 3pro (USA), which is an isokinetic measuring device, was used to examine the flexion and extension forces of the knee joint. During isokinetic muscle strength evaluation, the ROM of US was set to be the same as that of IS for consistency of measurement. [Results] At 60°/s, the isokinetic muscle functions of the females did not show any significant change between before and after surgery in any of the variables on both US and IS. At 60°/s, the isokinetic muscle functions of the males did not show any significant change between before and after surgery in the peak torque, average power, and entire work done on US. In extension, peak torque on IS did not show any significant change.

著者

Tanaka Taka-aki

出版者

Institute of Mathematics, University of Tsukuba

雑誌

Tsukuba journal of mathematics (ISSN:03874982)

巻号頁・発行日

vol.30, no.2, pp.345-361, 2006-12

著者

Takibuchi Gaku Imanishi Hirotake Morimoto Mami Ishikawa Kaori Nakada Kazuto Toyama-Sorimachi Noriko Kikkawa Yoshiaki Takenaga Keizo Hayashi Jun-Ichi

出版者

Elsevier Science

雑誌

Mitochondrion (ISSN:15677249)

巻号頁・発行日

vol.13, no.6, pp.881-887, 2013-11

被引用文献数

8 2

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To examine whether polymorphic mtDNA mutations that do not induce significant respiration defects regulate phenotypes of tumor cells, we used mouse transmitochondrial tumor cells (cybrids) with nuclear DNA from C57BL/6 (B6) strain and mtDNA from allogenic C3H strain. The results showed that polymorphic mutations of C3H mtDNA in the cybrids induced hypoxia sensitivity, resulting in a delay of tumor formation on their subcutaneous inoculation into B6 mice. Therefore, the effects of polymorphic mutations in normal mtDNA have to be carefully considered, particularly when we apply the gene therapy to the embryos to replace their pathogenic mtDNA by normal mtDNA.

著者

Sasaki Go Ishiwata Keisuke Machida Ryuichiro Miyata Takashi Su Zhi-Hui

出版者

BioMed Centra

雑誌

BMC evolutionary biology

巻号頁・発行日

vol.13, pp.236, 2013-10

被引用文献数

35

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BackgroundMolecular phylogenetic analyses have revealed that Hexapoda and Crustacea form a common clade (the Pancrustacea), which is now widely accepted among zoologists; however, the origin of Hexapoda remains unresolved. The main problems are the unclear relationships among the basal hexapod lineages, Protura (proturans), Collembola (springtails), Diplura (diplurans), and Ectognatha (bristletails, silverfishes, and all winged insects). Mitogenomic analyses have challenged hexapod monophyly and suggested the reciprocal paraphyly of Hexapoda and Crustacea, whereas studies based on nuclear molecular data support the monophyletic origin of hexapods. Additionally, there are significant discrepancies with respect to these issues between the results of morphological and molecular studies. To investigate these problems, we performed phylogenetic analyses of Pancrustacea based on the protein sequences of three orthologous nuclear genes encoding the catalytic subunit of DNA polymerase delta and the largest and second largest subunits of RNA polymerase II from 64 species of arthropods, including representatives of all hexapod orders.ResultsPhylogenetic analyses were conducted based on the inferred amino acid (aa) sequences (~3400 aa in total) of the three genes using the maximum likelihood (ML) method and Bayesian inference. Analyses were also performed with additional datasets generated by excluding long-branch taxa or by using different outgroups. These analyses all yielded essentially the same results. All hexapods were clustered into a common clade, with Branchiopoda as its sister lineage, whereas Crustacea was paraphyletic. Within Hexapoda, the lineages Ectognatha, Palaeoptera, Neoptera, Polyneoptera, and Holometabola were each confirmed to be monophyletic with robust support, but monophyly was not supported for Entognatha (Protura + Collembola + Diplura), Ellipura (Protura + Collembola), or Nonoculata (Protura + Diplura). Instead, our results showed that Protura is the sister lineage to all other hexapods and that Diplura or Diplura + Collembola is closely related to Ectognatha.ConclusionThis is the first study to include all hexapod orders in a phylogenetic analysis using multiple nuclear protein-coding genes to investigate the phylogeny of Hexapoda, with an emphasis on Entognatha. The results strongly support the monophyletic origin of hexapods but reject the monophyly of Entognatha, Ellipura, and Nonoculata. Our results provided the first molecular evidence in support of Protura as the sister group to other hexapods. These findings are expected to provide additional insights into the origin of hexapods and the processes involved in the adaptation of insects to life on land.

著者

Park Wug-Dong Tanioka Kenkichi

出版者

Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physics

雑誌

Japanese journal of applied physics. Pt. 1, Regular papers & short notes (ISSN:00214922)

巻号頁・発行日

vol.42, no.4, pp.1954-1956, 2003-04-15

被引用文献数

11

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In this paper, spectral responses of Te-doped a-Se HARP (High-gain Avalanche Rushing amorphous Photoconductor) thin films for a solid state image sensor have been reported. Te concentrations of Te-doped layer in a-Se HARP thin film were 15 wt.% and 26 wt.%, and thicknesses of Te-doped layer were 60 nm, 90 nm, and 120 nm. Spectral responses of Te-doped a-Se HARP films were investigated at bias voltages of 40 V and 60 V. Relative sensitivity and quantum efficiency of a-Se HARP films at 60 V were found to be improved by the increase of Te-doped layer thickness. This improvement is explained by the increased photogeneration efficiency at long wavelength region by the increase of Te-doped layer thickness and avalanche multiplication of the photogenerated carriers at a high electric field.

著者

CHEN Chien-Ming KU Wei-Chi

出版者

一般社団法人電子情報通信学会

雑誌

IEICE transactions on communications (ISSN:09168516)

巻号頁・発行日

vol.85, no.11, pp.2519-2521, 2002-11-01

被引用文献数

26

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Recently, Lin et al. addressed two weaknesses of a new strong-password authentication scheme, the SAS protocol, and then proposed an improved one called the OSPA (Optimal Strong-Password Authentication) protocol. However, we find that both the OSPA protocol and the SAS protocol are vulnerable to the stolen-verifier attack.

著者

SHIMIZU Satoko TSUKADA Toshihiko FUTAMI Hitoyasu UI Kotaro KAMEYA Toru KAWANAKA Masahiro UCHIYAMA Shozo AOKI Akira YASUDA Hiroko KAWANO Shin-ichiro ITO Yukio KANBE Masako OBARA Takao YAMAGUCHI ken

雑誌

Japanese journal of cancer research : gann (ISSN:09105050)

巻号頁・発行日

vol.88, no.11, pp.1029-1032, 1997-11-30

被引用文献数

20 33

著者

林光 作曲 谷川俊太郎 訳詩 藤本高茂 [第6-7 11 13曲] ひばり児童合唱団 歌 コンセール・ボウ・オーケストラ 林光 指揮

出版者

CBS/Sony

巻号頁・発行日

1976

著者

edited by A. Shisha-Halevy

出版者

Peeters

巻号頁・発行日

1988

著者

by A. Shisha-Halevy

出版者

Peeters

巻号頁・発行日

2007

著者

Ariel Shisha-Halevy

出版者

Pontificium Institutum Biblicum

巻号頁・発行日

1986

著者

Dong Han Choi Jae Hoon Noh Jung-Hyun Lee

出版者

日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

pp.ME13063, (Released:2013-12-28)

被引用文献数

2 10

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Synechococcus are distributed throughout the world’s oceans and are composed of diverse genetic lineages. However, as they are much less abundant than Prochlorococcus in oligotrophic open oceans, their in-depth genetic diversity cannot be investigated using commonly used primers targeting both Prochlorococcus and Synechococcus. Thus, in this study, we designed a primer specific to the 16S-23S rRNA internal transcribed spacer (ITS) of the Synechococcus subcluster 5.1. Using the primer, we could selectively amplify Synechococcus sequences in oligotrophic seawater samples. Further, we showed that a barcoded amplicon pyrosequencing method could be applicable to investigate Synechococcus diversity using sequences retrieved in GenBank and obtained from environmental samples. Allowing sequence analyses of a large number of samples, this high-throughput method would be useful to study global biodiversity and biogeographic patterns of Synechococcus in marine environments.

著者

花岡 利幸 楊 進春 趙 文謙 西井 和夫 北村 眞一 ZHAO Wen-qian YANG Jin-chun 中村 文雄 今岡 正美 竹内 邦良 荻原 能男 張 道成

出版者

山梨大学

雑誌

国際学術研究

巻号頁・発行日

1995

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1、自然災害への対策:甲府盆地及び四川盆地を流れる河川は氾濫河川であり、両地域とも治水技術に長い歴史と特徴を持っている。2、市街地拡大・人口集中への対策:甲府盆地では城下町、周辺の農村集落の成立を通じて緩やかな発展(時速4km/hrの世界)を遂げてきたが、1965年以降の自動車化(時速40km/hr)によって市街地の拡大が始まった。自動車保有の増大に合わせる需要追随型道路計画が進められてきた。1980年代の高速交通時代(時速120km/hr)の始まりは、計画先行型計画を取り込む気運を育み、新環状道路建設計画(1994)に繋がった。成都市は約40年間に人口の都市集中が起こり、人口960.4万人、市区人口304.5万人の中国内陸部の大都市に成長した。その結果、中心部の収容力に対し人口負荷が上回り、交通渋滞、エネルギー資源不足、通学難などの問題が顕著になった。自転車中心から自動車時代を目前に控え、交通問題は深刻さを増している。対策として環状道路建設、地下鉄建設、衛星都市・中小都市の育成による大都市への人口集中の抑制が急務となっている。3、都市中心部の総合整備事業:成都市では中心部外縁を全長29kmの府河南河が流下し、「二江抱城」の独特な景観を呈している。歴代の府南河は水が清く深く、潅漑、供水、運航、洪水防御などの機能を持ち、主要交通路でもあった。しかし、工業の発展、人口の増加に伴い、府南河はスラム地と化し、昔の栄光を失いつつある。府南河整備事業は、生態的都市の建設を目指し、持続的経済成長および社会的進歩を推進する目的の総合整備事業である。その内容は(1)洪水防御、(2)環境保全、(3)緑地整備、(4)道路とライフラインの整備、(5)住宅建設などである。沿河住民10万人移住を伴うこの事業は1985年から計画検討の後、1992年に正式に着工し、5年の歳月を経て1997年に概成した。その社会経済的および環境の効果が顕著に現れている。

著者

Jisun KIM Seong Young KWON Rohani CENA Seungjo PARK Juyeon OH Heejin OUI Kyoung-Oh CHO Jung-Joon MIN Jihye CHOI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0434, (Released:2013-12-31)

被引用文献数

9

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A 10-year-old, intact female Yorkshire terrier had multiple pulmonary nodules on thoracic radiography and ultrasonography, with no lesions elsewhere. Computed tomography (CT) and positron emission tomography and computed tomography (PET-CT) using 18F-fluorodeoxyglucose (FDG) were performed to identify metastasis and undetected primary tumors. On CT examination, pulmonary nodules had a hypoattenuating center with thin peripheral enhancement, suggesting ischemic or necrotizing lesion. In PET-CT at 47 min after intravenous injection of 11.1 MBq/kg of FDG, the maximum standardized uptake value of each pulmonary nodule was about from 3.8 to 6.4. There were no abnormal lesions except for four pulmonary nodules on the CT and PET-CT. Primary lung tumor was tentatively diagnosed and palliative therapy using 2 mg/kg tramadol and 2.2 mg/kg carprofen twice per day was applied. After the dog’s euthanasia due to deteriorated clinical signs and poor prognosis, undifferentiated pulmonary adenocarcinoma was diagnosed through histopathologic and immunochemistry examination. To the best of the authors’ knowledge, this is the first study of CT and PET-CT features of canine pulmonary adenocarcinoma. In this case, multiple pulmonary adenocarcinoma could be determined on the basis of FDG PET-CT through screening the obvious distant metastasis and/or lymph node invasions and excluding unknown primary tumors.

著者

David J. Castillo Shuichi Nakamura Yusuke V. Morimoto Yong-Suk Che Nobunori Kami-ike Seishi Kudo Tohru Minamino Keiichi Namba

出版者

日本生物物理学会

雑誌

BIOPHYSICS (ISSN:13492942)

巻号頁・発行日

vol.9, pp.173-181, 2013 (Released:2013-12-26)

参考文献数

46

被引用文献数

4 34

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The bacterial flagellar motor is made of a rotor and stators. In Salmonella it is thought that about a dozen MotA/B complexes are anchored to the peptidoglycan layer around the motor through the C-terminal peptidoglycan-binding domain of MotB to become active stators as well as proton channels. MotB consists of 309 residues, forming a single transmembrane helix (30-50), a stalk (51-100) and a C-terminal peptidoglycan-binding domain (101-309). Although the stalk is dispensable for torque generation by the motor, it is required for efficient motor performance. Residues 51 to 72 prevent premature proton leakage through the proton channel prior to stator assembly into the motor. However, the role of residues 72-100 remains unknown. Here, we analyzed the torque-speed relationship of the MotB(Δ72-100) motor. At a low speed near stall, this mutant motor produced torque at the wild-type level. Unlike the wild-type motor, however, torque dropped off drastically by slight decrease in external load and then showed a slow exponential decay over a wide range of load by its further reduction. Since it is known that the stator is a mechanosensor and that the number of active stators changes in a load-dependent manner, we interpreted this unusual torque-speed relationship as anomaly in load-dependent control of the number of active stators. The results suggest that residues 72-100 of MotB is required for proper load-dependent control of the number of active stators around the rotor.

著者

Jean-Paul Brodeur editor

出版者

Avebury

巻号頁・発行日

1995

著者

Geneviève Lacambre with contributions by Larry J. Feinberg Marie-Laure de Contenson and Douglas W. Druick

出版者

The Art Institute of Chicago, in association with Princeton University Press

巻号頁・発行日

1999

著者

Mayumi NAKAJIMA Koichi OHNO Yuko GOTO-KOSHINO Yasuhito FUJINO Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0583, (Released:2013-12-20)

被引用文献数

3 9

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Rapid turnover proteins such as transferrin (Tf) are used as dynamic nutritional assessment proteins in human medicine. However, nutritional status in veterinary medicine is mostly assessed on the basis of classical static factors such as body weight, body condition score and plasma albumin level. This study evaluated the clinical usefulness of Tf as nutritional assessment marker by measuring plasma Tf concentrations in malnourished dogs before and after nutritional treatment. Post plasma Tf concentrations were significantly higher than those of pre treatment, although albumin concentration did not change significantly. Numbers of dogs that exhibited increases in plasma Tf concentrations are significantly related to weight gain. Furthermore, the survival rates at day 60 after the treatment initiation were significantly higher in dogs with plasma Tf concentrations above the reference value (180 mg/dl) after the nutritional treatment than those with a plasma Tf concentration<180 mg/dl. In conclusion, plasma Tf concentration is related to nutritional condition and would be a candidate for novel nutritional assessment marker in dogs.