著者

Yuki Sato-Takabe Koji Hamasaki Koji Suzuki

出版者

日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.29, no.1, pp.100-103, 2014 (Released:2014-04-02)

参考文献数

17

被引用文献数

4 12

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This paper describes the photosynthetic response of a Roseobacter strain of marine aerobic anoxygenic phototrophic bacteria to an organic substrate limitation. In batch cultures, higher values of the spheroidenone/bacteriochlorophyll a ratio were observed under substrate-deficient conditions. Interestingly, the maximum photochemical quantum efficiencies of the photosystem under substrate-deficient conditions using blue or green excitation were significantly higher than those under substrate-replete conditions. These results indicate that spheroidenone, which can absorb green light, may play an important role in their photosynthesis as a light-harvesting antenna pigment, and the photosynthetic competence of the Roseobacter strain can increase in an organic substrate-deficient environment.

著者

Dong Han Choi Jae Hoon noH Jung-Hyun Lee

出版者

日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.29, no.1, pp.17-22, 2014 (Released:2014-04-02)

参考文献数

36

被引用文献数

2 10

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Synechococcus are distributed throughout the world’s oceans and are composed of diverse genetic lineages. However, as they are much less abundant than Prochlorococcus in oligotrophic open oceans, their in-depth genetic diversity cannot be investigated using commonly used primers targeting both Prochlorococcus and Synechococcus. Thus, in this study, we designed a primer specific to the 16S–23S rRNA internal transcribed spacer (ITS) of the Synechococcus subcluster 5.1. Using the primer, we could selectively amplify Synechococcus sequences in oligotrophic seawater samples. Further, we showed that a barcoded amplicon pyrosequencing method could be applicable to investigate Synechococcus diversity using sequences retrieved in GenBank and obtained from environmental samples. Allowing sequence analyses of a large number of samples, this high-throughput method would be useful to study global biodiversity and biogeographic patterns of Synechococcus in marine environments.

著者

Witold S. Proskura Daria Cichoń Wilhelm Grzesiak Daniel Zaborski Ewa Sell-Kubiak Yeong-Hsiang Cheng Andrzej Dybus

出版者

日本家禽学会

雑誌

The Journal of Poultry Science (ISSN:13467395)

巻号頁・発行日

pp.0130237, (Released:2014-03-25)

被引用文献数

15

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The objective of the present study was to investigate the relationship between the g.2582481G>A, g.2583935G>A and g.2584057C>T single nucleotide polymorphisms (SNPs) within the lactate dehydrogenase A gene (LDHA) coding for lactate dehydrogenase isoform A and the racing performance of homing pigeons. As a measure of racing performance, we used the mean values of ace points won by individual birds during the whole season. The estimated heritability of the racing performance of pigeons was relatively low (h2=0.0596; SE=0.0249). The analyzis performed for all race reports together showed that the factors such as gender, weather conditions at the start and at the end of the race affect the analyzed trait. Of the 3 single nucleotide polymorphisms, only the effect of the g.2582481G>A genotype on the performance of racing pigeons was significant. Statistical analyzis indicated the difference in the value of ace points between the animals of GG and GA genotypes for the g.2582481G>A SNP. The study showed that the genotype homozygous for g.2582481A is linked to the highest mean value of ace points. Consequently, the relationship between the genotype for g.2582481G>A and the racing performance was shown.

著者

右田 たい子 藤井 浩 Hong Zhou 吉田 匡 Olson John S. Ikeda-Saito Masao

出版者

一般社団法人日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.37, no.2, 1997-09-05

著者

柴崎 達雄 日本-インド"カダッパァ盆地"共同研究グループ

出版者

日本地質学会

雑誌

日本地質学会学術大会講演要旨

巻号頁・発行日

vol.92, 1985-03-25

著者

Yoshimi Kobayashi Makoto Hayashi Fumihiko Yoshino Muneaki Tamura Ayaka Yoshida Haruna Ibi Masaichi-Chang-il Lee Kuniyasu Ochiai Bunnai Ogiso

出版者

日本大学歯学部

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.56, no.1, pp.35-39, 2014 (Released:2014-03-15)

参考文献数

28

被引用文献数

2 12

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Chemomechanical procedures can be used to eliminate bacteria from root canals. However, detectable bacteria sometimes remain because of the complexity of the root canal system. Endodontic passive ultrasonic irrigation (PUI) with hydrogen peroxide (H2O2) may be a promising option for increasing bactericidal hydroxyl radical (HO•) generation. In this in vitro experiment, we examined the effects of HO• generated using PUI and a low concentration of H2O2. An ultrasonic tip was submerged in 0.45 mol/L (1.5%) H2O2 in a microfuge tube. H2O2 was activated by an ultrasonic unit, the tip of which was kept centered in the tube, to mimic PUI. HO• generation was detected by electron spin resonance spectroscopy. An Enterococcus faecalis suspension in H2O2 was then preparedand activated as described above. Bactericidal effects were assessed by viable counting. Two-way analysis of variance and Tukey’s test were used to assess the statistical significance of differences among groups (P < 0.05). HO• generation and bactericidal activity were significantly increased by PUI in H2O2 in a time-dependent manner and were significantly higher than with H2O2 alone or with PUI in a Tris-HCl suspension. These results suggest that PUI in the presence of a low H2O2 concentration is a promising new disinfection strategy. (J Oral Sci 56, 35-39, 2014)

著者

Po-Yu Liu Chin-Fu Lin Kwong-Chung Tung Ching-Lin Shyu Ming-Ju Wu Jai-Wen Liu Chi-Sen Chang Kun-Wei Chan Jin-An Huang Zhi-Yuan Shi

出版者

一般社団法人 日本内科学会

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.52, no.4, pp.431-438, 2013 (Released:2013-02-15)

参考文献数

50

被引用文献数

6 43

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Objective Shewanella bacteremia is an uncommon but potentially fatal disease. Although hepatobiliary diseases have been proposed to be risk factors for various Shewanella infections, little is known about the features of Shewanella bacteremia in patients with hepatobiliary diseases. This study aims to characterize the presentation and risk factors of Shewanella bacteremia in patients with hepatobiliary diseases. Methods We retrospectively investigated the clinical features, microbiology and outcomes of patients with Shewanella bacteremia who were admitted to a tertiary medical center between January 2001 and December 2010. All isolates were confirmed to the species level using 16S rRNA sequencing analyses. The English language medical literature was searched for previously published reports. Results Fifty-nine cases of Shewanella bacteremia, including nine at the hospital, were identified, 28 (47.4%) of which involved underlying hepatobiliary diseases, representing an important risk factor. In 12 of the 28 cases, the infections involved the hepatobiliary system; with a tendency towards an Asian origin. In our case series of nine patients, Shewanella haliotis was isolated in five patients. The majority of our patients lived in coastal areas, consumed seafood regularly and developed bacteremia during the summer season. Conclusion It is recommended that the possibility for Shewanella infection be considered in patients with bacteremia and also underlying hepatobiliary diseases, particularly if patients present with hepatobiliary infections, a history of seafood, or development of the disease during the summer.

著者

Kim Ki Jung Cho Hyeong Seok Choi Se Joon Jeun Seung Hyun Kim Seong Yun Sung Ki-Wug

出版者

The Japanese Pharmacological Society

雑誌

Journal of Pharmacological Sciences (ISSN:13478613)

巻号頁・発行日

vol.107, no.1, pp.57-65, 2008

被引用文献数

5

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The pharmacological action of riluzole, a drug that has been approved as a neuroprotective agent for the treatment of amyotrophic lateral sclerosis, has not yet been established. We examined the effects of riluzole on 5-hydroxytryptamine (5-HT)₃ receptors in NCB-20 neuroblastoma cells using the whole-cell voltage clamp technique combined with a fast drug application method. Co-application of riluzole (1 – 300 μM, 5 s) produced a dose-dependent reduction in peak amplitudes and in the rise slope of the currents induced by 2 μM 5-HT. In addition, 5-HT₃–mediated currents evoked by dopamine, a partial 5-HT₃–receptor agonist, were inhibited by riluzole co-application. These inhibitory effects were clearly shown at low concentrations of 5-HT. The decay time constants of the receptor desensitization and deactivation were also significantly attenuated by riluzole. G-protein inhibitors (pertussis toxin and guanosine 5'-[β-thio] diphosphate) did not completely block these inhibitory actions of riluzole. These results indicate that riluzole inhibits 5-HT₃–induced ion currents directly by slowing channel activation in NCB-20 neuroblastoma cells.<br>

著者

Nestle-Aland herausgegeben von Barbara und Kurt Aland ...

出版者

Deutsche Bibelgesellschaft

巻号頁・発行日

2012

著者

Muneki HONNAMI Sungjin CHOI I-li LIU Wataru KAMIMURA Tetsushi TAGUCHI Hironori HOJO Nobuyuki SHIMOHATA Shinsuke OHBA Hiroyuki KOYAMA Ryohei NISHIMURA Ung-il CHUNG Nobuo SASAKI Manabu MOCHIZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0027, (Released:2014-03-26)

被引用文献数

1 5

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The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones® [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created in the radius of 5 dogs, stabilized with a plate and screws, and implanted with 1 of the following: TB (TB group), TB and bFGF solution (TB/f group), and TB and f-IC gel (TB/f-IC group). Dogs were euthanized 4 weeks after surgery. Radiographs showed well-placed TB granules in the defects and equal osseous callus formation in all the groups. Histomorphometry revealed that the number of vessels and volume of new bone in the TB/f-IC group were significantly higher than those in the other groups. However, no significant differences in neovascularization and new bone formation were observed between the TB/f and TB groups. Furthermore, no significant difference in the lamellar bone volume or rate of mineral apposition was observed among groups. These results suggest that increased bone formation might have been because of the promotion of neovascularization by the f-IC gel. Therefore, the combinatorial method may provide a suitable scaffold for bone regeneration in large segmental long bone defects.

著者

Kaori Nakanishi Makoto Nishida Tohru Ohama Toshiki Moriyama Keiko Yamauchi-Takihara

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-13-1134, (Released:2014-03-11)

参考文献数

33

被引用文献数

5 21

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Background: Smoking and metabolic syndrome (MetS) are major public health problems in modern society and are important risk factors of cardiovascular disease (CVD). The association of smoking, MetS, and CVD is widely reported, but reports targeted to women are few. In the present study, we evaluated risk factors, including visceral fat area (VFA), for CVD and development of subclinical atherosclerosis in female smokers especially. Methods and Results: Subjects consisted of 162 apparent healthy female and male smokers, and 315 age-matched never-smokers who underwent a health examination in the Osaka University Health Care Center. For female smokers, lifestyle and carotid intima-media thickness (IMT) were evaluated. Triglycerides were significantly higher and high-density lipoprotein-cholesterol significantly lower in smokers than in never-smokers for both men and women. However, VFA was significantly high only in smoking women when compared with never-smokers. Multivariate analysis revealed that age, body mass index, and smoking were the independent predictors of high VFA in women. In addition, annual IMT increase was significantly higher in smokers than never-smokers in women. Conclusions: VFA was notably high in female smokers, but the difference was not observed in men. Smoking habit is an important risk factor of visceral fat accumulation and progression of subclinical atherosclerosis in women.

著者

Toshiyuki Masuzawa Yoshiyuki Uchishima Takashi Fukui Yoshihiro Okamoto Ming-Jeng Pan Teruki Kadosaka Nobuhiro Takada

出版者

国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.67, no.2, pp.111-114, 2014 (Released:2014-03-20)

参考文献数

23

被引用文献数

3 18

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A total of 138 animals from 7 species (Apodemus agrarius, Bandicota indica, Crocidura suaveolens, Mus caroli, Mus formosanus, Rattus losea, and Suncus murinus) captured in Taichung, located in central Taiwan, and Kinmen Island, an island off the shore of China, were examined for the presence of Anaplasma phagocytophilum. The presence of the bacteria, which causes human granulocytic anaplasmosis, was examined by nested PCR targeting the16S rDNA. Twelve animals (8.7%) from M. caroli and R. losea, and 25 (18.1%) from A. agrarius, B. indica, M. caroli, and R. losea were infected with A. phagocytophilum and Anaplasma bovis, respectively. Phylogenetic analysis revealed that partial 16S rDNA sequences in the 12 aforementioned animals showed higher similarity to the sequences related to A. phagocytophilum detected in wild rodents (Rattus and Niviventer) from southeast China. The sequences of the other 25 animals belonged to the A. bovis clade. We demonstrated that small wild mammals were infected with A. phagocytophilum and A. bovis in Taichung and Kinmen Island, Taiwan.

著者

Hee-Chun LEE Ji-Hyun KIM Cho-Hee JEE Jae-Hoon LEE Jong-Hyun MOON Na-Hyun KIM Jung-Hyang SUR Kyu-Woan CHO Byeong-Teck KANG Jeongim HA Dong-In JUNG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0315, (Released:2014-03-20)

被引用文献数

1 8

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A 9-year-old castrated male Shih-tzu dog was referred to us because of chronic vomiting. The patient’s hematological, radiographic, ultrasonographic, endoscopic, and histological examinations were evaluated for diagnosis. Hematologic analysis indicated moderate anemia and azotemia. Based on imaging study, oval-shape mass was identified in gastric pylorus area. A proliferative mass was found on endoscopic examination and we performed biopsy using grasping forceps. The histopathological findings of the biopsy specimens indicated hypertrophic gastritis and Y-U pyloroplasty was performed. However, the histopathological examination of surgically resected mass was identified as tubular adenocarcinoma of stomach. Then carboplatin chemotherapy was performed 4 times for 13 weeks. The clinical sign such as vomiting were resolved gradually after surgery and chemotherapy, and the patient’s condition was managed favorably until recently (30 months after surgery). This case report demonstrated clinical features, imaging studies, endoscopic characteristics, histopathological and immunohistochemical features of gastric tubular adenocarcinoma as early gastric cancer in a dog.

著者

Sang-Heon Lee

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.26, no.2, pp.239-241, 2014 (Released:2014-02-28)

参考文献数

20

被引用文献数

1 2

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[Purpose] The purpose of this study was to analyze the activity engagement of the retiree population in South Korea. [Methods] The Korean-Activity Card Sort (K-ACS) was used to collect research data. A One-way ANOVA and post-hoc comparisons showed significant group effects among three age groups. The independent t-tests was used to analyze the differences in mean retained level of activity (MRA) between men and women. [Results] The one-way ANOVA showed statistically significant differences in MRA among different age groups. Scheffé’s test revealed a statistically significant decrease in MRA in Group A, aged more than 75 years, as compared to the other two age groups. When participants were divided by gender, MRA of instrumental activities showed a statistically significant difference between the 65–74 years group and the 55–65 years group, but no difference in females of the leisure activities among the age groups. The independent t-tests demonstrated significant gender differences in MRA of activity of the 55–64 years group. [Conclusion] These findings suggest that health professionals should monitor the changes in retained level of activity after age 75, to maintain their engagement, and the importance of age-, gender- and activity-specific analyses in order to identifying patterns of activity engagement.

著者

Toyotaka SATO Shin-ichi YOKOTA Risa ICHIHASHI Tomoka MIYAUCHI Torahiko OKUBO Masaru USUI Nobuhiro FUJII Yutaka TAMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0144, (Released:2014-03-18)

被引用文献数

7

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Understanding the prevalence of antimicrobial-resistance and the relationship between emergence of resistant bacteria and clinical treatment can facilitate design of effective treatment strategies. We here examined antimicrobial susceptibilities of Escherichia coli isolated from dogs admitted to a university hospital (University hospital) and companion animal clinics (Community clinics) in the same city, and investigated underlying multidrug-resistance mechanisms. The prevalence of E. coli with intermediate and resistant interpretations to ampicillin (AMP), enrofloxacin (ENR) and chloramphenicol (CHL) was higher in the University hospital than in the Community clinics cases. Use of antimicrobials, including fluoroquinolone, was also significantly higher in the University hospital than in the Community clinics cases. Upon isolation using ENR-supplemented agar plates, all ENR-resistant isolates had 3–4 nucleotide mutations that accompanied by amino acid substitutions in the quinolone-resistance-determining regions of gyrA, parC and parE, and 94.7% of all isolates derived from the University hospital showed AMP and/or CHL resistance, and possessed blaTEM and/or catA1. The average mRNA expression levels of acrA, acrB and tolC, and the prevalence of organic solvent tolerance, in isolates derived from ENR-supplemented agar plates were significantly higher in the University hospital than in the Community clinics isolates. Thus, E. coli derived from the University hospital cases more often showed concomitant decreased susceptibilities to aminopenicillins, fluoroquinolones, and CHL than did those derived from the Community clinics; this was related to an active AcrAB–TolC efflux pump, in addition to acquisition of specific resistance genes and genetic mutations.

著者

Hassadin BOONSRIROJ Kazunori KIMITSUKI Tetsuya AKAGI Chun-Ho PARK

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0057, (Released:2014-03-17)

被引用文献数

1 6

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A malignant epithelioid schwannoma of the oral cavity was diagnosed in an 8-year-old domestic short-hair cat. The mass was located in the gingiva of the upper left premolar to molar region and showed multinodular growth patterns. The mass comprised epithelioid cells arranged in densely packed sheets. Tumor cells had large, round to oval nuclei with prominent nucleoli and an abundant eosinophilic cytoplasm. Immunohistochemically, most of the tumor cells were positive for S-100 protein, glial fibrillary acidic protein, and vimentin, but all lacked melanoma-associated antigen and muscle and neuroendocrine markers. Stains for type IV collagen showed linear immunoreactivity around single cells and groups of cells. Ultrastructurally, tumor cells were separated by a well-defined basement membrane, and interdigitating cell processes were observed. To our knowledge, this is first report of feline malignant epithelioid schwannoma.

著者

Guo Rong-Jun Arai Hajime Kitayama Yasuhiko IGARASHI Hisaki HEMMI Hiromichi ARAI Tomio HANAI Hiroyuki SUGIMURA Haruhiko

出版者

浜松医科大学

雑誌

Pathology international (ISSN:13205463)

巻号頁・発行日

vol.51, no.4, pp.240-247, 2001-04-01

被引用文献数

6 30

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浜松医科大学学位論文 医博第322号(平成13年03月27日)

著者

Yuuichi Nakano Mitsuo Iwadate Hideaki Umeyama Y-h. Taguchi

出版者

Information and Media Technologies 編集運営会議

雑誌

Information and Media Technologies (ISSN:18810896)

巻号頁・発行日

vol.9, no.1, pp.141-154, 2014 (Released:2014-03-15)

参考文献数

15

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Type III secretion system (T3SS) effector protein is a part of bacterial secretion systems. T3SS exists in the pathogenic and symbiotic bacteria. How the T3SS effector proteins in these two classes differ from each other should be interesting. In this paper, we successfully discriminated T3SS effector proteins between plant pathogenic, animal pathogenic and plant symbiotic bacteria based on feature vectors inferred computationally by Yahara et al. only from amino acid sequences. This suggests that these three classes of bacteria employ distinct T3SS effector proteins. We also hypothesized that the feature vector proposed by Yahara et al. represents protein structure, possibly protein folds defined in Structural Classification of Proteins (SCOP) database.

著者

Gregory Poole Nagy El-kaddah

出版者

一般社団法人 日本鉄鋼協会

雑誌

ISIJ International (ISSN:09151559)

巻号頁・発行日

vol.54, no.2, pp.321-327, 2014-02-15 (Released:2014-03-11)

参考文献数

34

被引用文献数

2 12

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This paper examines the role of induction coil design on stirring of molten metal in electromagnetic (EM) solidification processes. A model is presented to describe the EM, heat transfer, and fluid flow phenomena in these processes. It is based on a dual-zone description of the mushy region, and accounts for damping of turbulence by the solidified crystallites. The electromagnetic field equations were solved using the mutual inductance technique, while the temperature and turbulent flow fields were calculated using the control volume method. Calculations were performed for solidification of an Al–Cu alloy placed in a stationary magnetic field generated by an induction coil. The effect of coil design on the flow structure was investigated for three different coil positions. It was found that changing the coil position significantly alters the flow pattern from four recirculating loops when the coil is above the midsection of the melt to two loops, typical of a travelling magnetic field, when the coil is at the base of the melt. This significantly modifies the rate of solidification across the ingot, as well as the temperature gradient, in the mushy region. The decay of the velocity and turbulent fields in the mushy region was found to be exponential, with the maximum rate of decay at the solidification front. These results indicate that through changes in coil design, it is possible to control the flow characteristics and solidification behavior in the molten metal.

著者

松浦 好治 鈴木 賢 宇田川 幸則 樋口 範雄 BENNETT F. G. Jr. 姜 東局 岡 克彦 外山 勝彦 小川 泰弘 角田 篤泰 増田 知子 中村 誠 佐野 智也 SHEE Huey-Ling HWANG Ren-Hung DING Xiang-shun LEE Heejeoung

出版者

名古屋大学

雑誌

基盤研究(S)

巻号頁・発行日

2011-04-01

---

本研究は、日中韓台・漢字文化圏の法情報について、深い相互理解と比較法研究の推進を目的とし、次の成果を得た。法情報共有の環 境整備として、各国研究者と共同で、中韓台法令とその英訳の対訳約14万文、英文官報の画像と日英対訳約16万文を集積するとともに、4法域法令用語標準対訳辞書の項目候補約13,000語の検討を推進した。また、日本法令の機械翻訳や文書構造化の手法を開発した。一方、分かりやすい法情報の提供事例として韓国とEUを調査するとともに、特定分野の理解を促進する法情報パッケージLawPackの例を構築した。また、地方自治体例規約98万本を蓄積・横断検索するシステムeLenを開発した。