- 著者
 
          - 
             
             Fukuda Yuuki
             
             Ando Yasuhiro
             
          
 
          
          
          - 出版者
 
          - Japanese Society of Fisheries Science
 
          
          
          - 雑誌
 
          - Fisheries Science (ISSN:09199268)
 
          
          
          - 巻号頁・発行日
 
          - vol.77, no.5, pp.875-882, 2011-09 
 
          
          
          
          - 被引用文献数
 
          - 
             
             
             4
             
             
          
        
 
        
        
        Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC–MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC–MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% (N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring.