著者

Kimura Ikuo Ozawa Kentaro Inoue Daisuke Imamura Takeshi Kimura Kumi Maeda Takeshi Terasawa Kazuya Kashihara Daiji Hirano Kanako Tani Taeko Takahashi Tomoyuki Miyauchi Satoshi Shioi Go Inoue Hiroshi Tsujimoto Gozoh

出版者

Nature Publishing Group

雑誌

Nature communications (ISSN:20411723)

巻号頁・発行日

vol.4, 2013-05-07

被引用文献数

1034

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腸内細菌による宿主のエネルギー恒常性維持機構の解明 : 短鎖脂肪酸受容体GPR43活性化は脂肪の蓄積を抑制し肥満を防ぐ. 京都大学プレスリリース. 2013-05-08.

著者

Takagi Keijiro WATANABE Minoru SAITO Hiroshi

出版者

公益社団法人 日本薬理学会

雑誌

The Japanese Journal of Pharmacology (ISSN:00215198)

巻号頁・発行日

vol.21, no.6, pp.797-810, 1971

被引用文献数

52

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It is the first and important practice for the study of the psychotropic drugs to observe the spontaneous movement of animals. Although many methods have been known for measuring the spontaneous movements, most of them are to find the change of motor activity of a single animal. Many investigators reported the automatic measurement of the motor activity. Photoelectric method was first used by Siegel et al. (1), which was improved by Dews et al. (2) in mice. Takagi et al. (3) traced the spontaneous movement of a mouse using a modified photoelectric apparatus.<BR> The central nervous system stimulant effect of 2-dimethylaminoethanol (DMAE) was first reported by Pfeiffer et al. (4) and had been confirmed by Konigsmark et al. (5), Himwich (6) and Brown and Gangloff (7). It was also confirmed by clinical trials. Pfeiffer et al. (8) proposed the hypothesis that DMAE played the role of a precursor of acetylcholine in the central nervous system. Groth et al. (9) found that DMAE was incorporated in the mouse brain more rapidly than choline and probably converted to brain choline. However, Pepeu et al. (10) and Kiplinger et al. (11) had different views from that of Pfeiffer.<BR> In this paper, we have developed a method of recording the spontaneous activity of grouped mice, in order to take an accurate measurement of a little change of motor activity by drugs. We investigated the effect of DMAE and its acyl esters on the spontaneous movement of the grouped and/or individual mice.

著者

Toyotaka SATO Shin-ichi YOKOTA Torahiko OKUBO Kanako ISHIHARA Hiroshi UENO Yasukazu MURAMATSU Nobuhiro FUJII Yutaka TAMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.4, pp.407-414, 2013 (Released:2013-05-02)

参考文献数

38

被引用文献数

17 30

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Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samples and canine fecal samples. E. coli strains with high levels of fluoroquinolone resistance have been found to be frequently resistant to cephalosporins. Strains with high-level fluoroquinolone resistance exhibited lower intracellular enrofloxacin (ENR) concentrations, higher expression of AcrA, and a greater reduction in the fluoroquinolone minimum inhibitory concentration for treatment with an efflux pump inhibitor. The frequency of strains with enhanced ENR resistance selection and the survival rate of E. coli in the presence of ENR in vitro were correlated well with AcrA protein expression levels in the parental strains. These results suggest that AcrAB-TolC efflux pump over-expression is related to high-level fluoroquinolone resistance and the selection of strains with enhanced fluoroquinolone resistance.

著者

Saito Keisuke Rutherford A William Ishikita Hiroshi

出版者

National Academy of Sciences

雑誌

Proceedings of the National Academy of Sciences of the United States of America (ISSN:00278424)

巻号頁・発行日

2013-04-18

被引用文献数

66

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光合成酸素発生反応で利用される蛋白質内のプロトン移動経路を発見 : 人工光合成系の設計やバイオエネルギーの生産性向上に指針. 京都大学プレスリリース. 2013-04-16.

著者

Ryozo Nagai Koichiro Kinugawa Hiroshi Inoue Hirotsugu Atarashi Yoshihiko Seino Takeshi Yamashita Wataru Shimizu Takeshi Aiba Masafumi Kitakaze Atsuhiro Sakamoto Takanori Ikeda Yasushi Imai Takashi Daimon Katsuhiro Fujino Tetsuji Nagano Tatsuaki Okamura Masatsugu Hori the J-Land Investigators

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.77, no.4, pp.908-916, 2013 (Released:2013-03-25)

参考文献数

24

被引用文献数

21 93

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Background: A rapid heart rate (HR) during atrial fibrillation (AF) and atrial flutter (AFL) in left ventricular (LV) dysfunction often impairs cardiac performance. The J-Land study was conducted to compare the efficacy and safety of landiolol, an ultra-short-acting β-blocker, with those of digoxin for swift control of tachycardia in AF/AFL in patients with LV dysfunction. Methods and Results: The 200 patients with AF/AFL, HR ≥120beats/min, and LV ejection fraction 25–50% were randomized to receive either landiolol (n=93) or digoxin (n=107). Successful HR control was defined as ≥20% reduction in HR together with HR <110beats/min at 2h after starting intravenous administration of landiolol or digoxin. The dose of landiolol was adjusted in the range of 1–10μg·kg–1·min–1 according to the patient’s condition. The mean HR at baseline was 138.2±15.7 and 138.0±15.0beats/min in the landiolol and digoxin groups, respectively. Successful HR control was achieved in 48.0% of patients treated with landiolol and in 13.9% of patients treated with digoxin (P<0.0001). Serious adverse events were reported in 2 and 3 patients in each group, respectively. Conclusions: Landiolol was more effective for controlling rapid HR than digoxin in AF/AFL patients with LV dysfunction, and could be considered as a therapeutic option in this clinical setting.  (Circ J 2013; 77: 908–916)

著者

常岡 和子 HIROSHI ISHIHARA ALEXANDER BORRISOV DIMCHEV KOJI NOMOTO TERUO YOKOKURA MIKIO SHIKITA

出版者

Journal of Radiation Research 編集委員会

雑誌

Journal of Radiation Research (ISSN:04493060)

巻号頁・発行日

vol.35, no.3, pp.147-156, 1994-09-15 (Released:2006-05-19)

参考文献数

25

被引用文献数

9 14

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A single subcutaneous injection of a preparation of heat-killed Lactobacillus casei (LC 9018), given before or after irradiation, significantly increased the survival rate of mice that had received 8.5-Gy 137Cs whole-body γ-irradiation. A similar radioprotective effect was observed when LC 9018 was administered within the period from 2 days before irradiation to 9 h after irradiation, the pre-irradiation treatment being slightly better than the post-irradiation treatment. Increases in the weight of the spleen and in the number of endogenous spleen colonies on days 8 and 12 after irradiation suggested that the radioprotective effect was based on enhanced recovery of hematopoietic tissues. The activity of macrophage colony-stimulating factor (M-CSF) in serum was rapidly increased by the treatment and was maintained at the elevated level for 13 days. At the same time, an increased level of M-CSF mRNA was detected in the livers of the treated mice. However, LC 9018 failed to save the lives of mice when administered 3 days after irradiation, although it increased serum M-CSF as effectively as noted above. The small advantage of the pre-irradiation over the post-irradiation treatment was not explained by the increases of metallothionein in the hematopoietic tissues of the treated mice.

著者

Miwa Yamaguchi Kokichi Arisawa Hirokazu Uemura Sakurako Katsuura-Kamano Hidenobu Takami Fusakazu Sawachika Mariko Nakamoto Tomoya Juta Eisaku Toda Kei Mori Manabu Hasegawa Masaharu Tanto Masayuki Shima Yoshio Sumiyoshi Kenji Morinaga Kazunori Kodama Takaichiro Suzuki Masaki Nagai Hiroshi Satoh

出版者

(公社)日本産業衛生学会

雑誌

Journal of Occupational Health (ISSN:13419145)

巻号頁・発行日

pp.12-0264-OA, (Released:2013-04-09)

被引用文献数

11 54

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Objective: Perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) have been shown to accumulate in the human body. The purpose of the present study was to examine the factors associated with the blood levels of PFOS and PFOA. Methods: A cross-sectional study was performed on 307 men and 301 women (aged 16-76 years) living in 15 prefectures in Japan. Blood levels of PFOS and PFOA were measured by liquid chromatography-mass spectrometry. Hepatic enzymes (γ-GTP, GOT, and GPT) and ω-3 polyunsaturated fatty acids (DHA and EPA) levels in serum were also measured. Associations between the levels of PFOS and PFOA in blood and the intake frequency of 41 kinds of dishes, foods and beverages and the serum levels of liver enzymes and ω-3 polyunsaturated fatty acids were examined using rank correlations. Results: Frequency of intake of boiled fish in broth, sliced raw fish and coastal fish showed significant positive correlations with PFOS concentrations in blood after adjustments for potential confounders. Serum levels of GOT, GPT, DHA and EPA showed significant positive correlations with PFOS and PFOA in blood. There was also a significant regional difference in the blood levels of PFOS and PFOA, with medians being highest in the Tokai/Hokuriku/Kinki region. Conclusion: These findings suggest that the concentrations of PFOS in blood were mainly associated with fish consumption and that the levels of PFOS and PFOA were mainly associated with the serum levels of liver enzymes in Japanese populations. Further investigations are required to clarify the reason for the regional differences in blood levels of PFOS and PFOA in Japan.

著者

安田 忠彰 三浦 祥吾 今井 理裕 井脇 洋 Yasuda tadaaki Miura shogo Imai Masahiro Iwaki Hiroshi

雑誌

【全国大会】平成20年電気学会全国大会論文集

巻号頁・発行日

pp.517-518, 2008-03-19

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都心系統には都心系統安定化リレーシステムが設置されており、万一の送電線事故時は系統内火力発電機と重要負荷で単独系統維持を図っている。この単独系統は一時的に運転維持に成功しても長時間の運転維持は困難であり、早急に本系統に系統並列する必要がある。従来、この系統並列操作は手動で実施していたが、これを自動で行うことで都心系統の迅速な本系統への系統並列を図る本装置を開発した。

著者

Li Yuanying Yamagishi Kazumasa Yatsuya Hiroshi Tamakoshi Akiko Iso Hiroyasu

出版者

Elsevier Inc.

雑誌

Preventive medicine (ISSN:00917435)

巻号頁・発行日

vol.55, no.6, pp.639-643, 2012-12

被引用文献数

11 4

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ObjectiveTo investigate an effect of smoking cessation on chronic obstructive pulmonary disease (COPD) mortality in Asians.MethodsThe data was obtained from the Japan Collaborative Cohort Study for Evaluation of Cancer Risk (JACC Study). A total of 41465 Japanese men and 52662 Japanese women aged 40–79 years who had no history of COPD, asthma, other chronic lung diseases, cardiovascular disease or cancer were followed between 1988 and 2008.ResultsDuring median 18-year of follow-up, there were 285 (251 men and 34 women) documented deaths from COPD. Multivariable-adjusted hazard ratios with 95% confidence intervals of COPD death were 4.46 (2.72–7.29) and 9.26 (4.19–20.5), respectively for current male and female smokers when compared to never smokers. Compared with current smokers, the multivariable HRs for 5–9 years and 10 years or more smoking cessation prior to baseline were 0.44 (0.22–0.87) and 0.36 (0.22–0.58) in men, respectively while the HR for never smokers was 0.30 (0.16–0.57). There were an insufficient number of COPD deaths in women to clarify this association.ConclusionSmoking cessation for ten years or more prior to enrollment reverses the excess risk of COPD mortality to a level similar to that observed among never smokers in men.

著者

Akiyoshi HAYASHI Hiroshi TANAKA Tomoko TAJIMA Masanari NAKAYAMA Fumihito OHASHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.3, pp.391-393, 2013 (Released:2013-04-01)

参考文献数

13

被引用文献数

6

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A 7-year-old spayed female domestic short-haired cat presented with dysuria and hematuria that had been unresponsive to medical therapy. Imaging tests such as ultrasonography, urethrocystography and computed tomography revealed a pelvic mass compressing the urethra. Based on histological examination of the mass following surgical resection, the cat was diagnosed squamous cell carcinoma (SCC) derived from the uterine remnant. After surgery, dysuria was resolved, but on instead, urine and fecal incontinence were observed. Then, about four months after surgery, recurrence of the mass and the symptoms was observed. Consequently, the cat was ultimately euthanized. This is the first report of SCC arising from the uterine remnant in a spayed female cat.

著者

Yuka HARA Junko SUZUKI Keita NOGUCHI Yutaka TERADA Hiroshi SHIMODA Takuya MIZUNO Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0003, (Released:2013-03-26)

被引用文献数

4 7

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Morbilliviruses use signaling lymphocyte activation molecule (SLAM) as a receptor for their entry to cells. In this study, a complete gene encoding SLAM of a domestic cat was identified. The identity of feline SLAM with canine one was 73%, and feline SLAM formed the same cluster with those of carnivores. Furthermore, feline cell expressing feline SLAM supported growth of canine distemper virus (CDV) as well as that expressing canine one. These results indicated that feline SLAM can function as a receptor for morbilliviruses, and our established feline cells that express feline SLAM might be useful for analysis of morbilliviruses originated from felids.

著者

Ryozo Nagai Koichiro Kinugawa Hiroshi Inoue Hirotsugu Atarashi Yoshihiko Seino Takeshi Yamashita Wataru Shimizu Takeshi Aiba Masafumi Kitakaze Atsuhiro Sakamoto Takanori Ikeda Yasushi Imai Takashi Daimon Katsuhiro Fujino Tetsuji Nagano Tatsuaki Okamura Masatsugu Hori the J-Land Investigators

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-12-1618, (Released:2013-03-15)

参考文献数

24

被引用文献数

21 93

---

Background: A rapid heart rate (HR) during atrial fibrillation (AF) and atrial flutter (AFL) in left ventricular (LV) dysfunction often impairs cardiac performance. The J-Land study was conducted to compare the efficacy and safety of landiolol, an ultra-short-acting β-blocker, with those of digoxin for swift control of tachycardia in AF/AFL in patients with LV dysfunction. Methods and Results: The 200 patients with AF/AFL, HR ≥120beats/min, and LV ejection fraction 25–50% were randomized to receive either landiolol (n=93) or digoxin (n=107). Successful HR control was defined as ≥20% reduction in HR together with HR <110beats/min at 2h after starting intravenous administration of landiolol or digoxin. The dose of landiolol was adjusted in the range of 1–10μg·kg–1·min–1 according to the patient’s condition. The mean HR at baseline was 138.2±15.7 and 138.0±15.0beats/min in the landiolol and digoxin groups, respectively. Successful HR control was achieved in 48.0% of patients treated with landiolol and in 13.9% of patients treated with digoxin (P<0.0001). Serious adverse events were reported in 2 and 3 patients in each group, respectively. Conclusions: Landiolol was more effective for controlling rapid HR than digoxin in AF/AFL patients with LV dysfunction, and could be considered as a therapeutic option in this clinical setting.

著者

Masa-aki Kishida Satoshi Muratsugu Ryota Sakamoto Tetsuro Kusamoto Hiroshi Nishihara

出版者

(社)日本化学会

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

pp.121264, (Released:2013-03-19)

被引用文献数

7

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We prepared dimethyldihydropyrene (DHP) derivative 2 which possesses two ferrocenyl groups at the 4 and 9 positions through ethynylene linkers. 2 showed intense electronic communication between the two ferrocenyl groups, which is promoted by the highly developed π-orbital of the diethynylDHP moiety.

著者

陳 瑞新 増田 高行 長沼 廣 Ruixin CHEN Takayuki MASUDA Hiroshi NAGANUMA

出版者

東北大学医療技術短期大学部

雑誌

東北大学医療技術短期大学部紀要 = Bulletin of College of Medical Sciences, Tohoku University (ISSN:09174435)

巻号頁・発行日

vol.3, no.2, pp.107-112, 1994-09-01

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甲状腺癌免疫組織化学法p53蛋白過剰発現

著者

Hachinohe Mayumi Yamane Midori Akazawa Daiki Ohsawa Kazuhiro Ohno Mayumi Terashita Yuzu Masumoto Hiroshi

出版者

Public Library of Science

雑誌

PLoS ONE (ISSN:19326203)

巻号頁・発行日

vol.8, no.1, pp.e54011, 2013-01

被引用文献数

14

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The regulation of energy metabolism, such as calorie restriction (CR), is a major determinant of cellular longevity. Although augmented gluconeogenesis is known to occur in aged yeast cells, the role of enhanced gluconeogenesis in aged cells remains undefined. Here, we show that age-enhanced gluconeogenesis is suppressed by the deletion of the tdh2 gene, which encodes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a protein that is involved in both glycolysis and gluconeogenesis in yeast cells. The deletion of TDH2 restores the chronological lifespan of cells with deletions of both the HST3 and HST4 genes, which encode yeast sirtuins, and represses the activation of gluconeogenesis. Furthermore, the tdh2 gene deletion can extend the replicative lifespan in a CR pathway-dependent manner. These findings demonstrate that the repression of enhanced gluconeogenesis effectively extends the cellular lifespan.

著者

万木 豊 永田 洋 Yurugi Yutaka Nagata Hiroshi

出版者

三重大学農学部

雑誌

三重大学農学部学術報告 (ISSN:04624408)

巻号頁・発行日

no.63, pp.p199-203, 1981-12

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この暖帯地方に分布する常緑広葉樹 4 種の長日条件と短日条件下での生長パターンをしらべ, その天然分布との関連を考察した。ヤマモモ・クスノキ, アラカンは長日条件, 短日条件下ともで周期的生長を示した。そこで, これら 3 樹種は亜熱帯地方では休眠に入ることなく, 分布可能なのであろう。タブノキは長日条件下で周期的生長を示すが, 短日条件下では 1 回のフラッシュをみたのみであった。このタブノキが亜熱帯地方に分布しているのは, 短日条件下でも周期的生長を示す生態型があるためであろう。Growth patterns of four evergreen broad-leaf trees distributed in this warm temperate zone on long-days and short-days were studied, and the relationship between growth patterns and distribution was discussed. Myrica rubra SIEB. Et ZUCC., Cinnamoum camphora SIEB., and Quercus glauca THUNB. showed periodic growth on long-days and even on short-days. So they can be distributed in the subtropical zone without being induced to bud dormancy. These three species seem to be induced to bud dormancy wit lowering temperatures in autumn, and to be released from bud dormancy with winter chilling in this region. Machilus thunbergii SIEB, et ZUCC. showed periodic growth on long-days, but showed only one flush and then no growth on short-days. It may be considered, therefore, that Machilus thunbergii can be distributed in the subtropical zone, having an ecotype showing periodic growth on short-days.

著者

寺岡 寛 Hiroshi Teraoka

雑誌

経済学論究 (ISSN:02868032)

巻号頁・発行日

vol.63, no.1, pp.1-30, 2009-06-15

著者

Ishitsuka Yosuke Kawachi Yasuhiro Taguchi Shijima Maruyama Hiroshi Fujisawa Yasuhiro Furuta Junichi Nakamura Yasuhiro Otsuka Fujio

出版者

Nature Publishing Group

雑誌

Journal of investigative dermatology (ISSN:0022202X)

巻号頁・発行日

vol.132, no.7, pp.1775-1784, 2012-07

被引用文献数

14 4

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The epidermis is a self-renewing tissue, the homeostasis of which is dependent upon the tight balance between proliferation and differentiation based on appropriate regulation of the cell cycle. The cell cycle regulation is dependent on the interactions among a number of cell cycle regulatory molecules, including the pituitary tumor-transforming gene 1 (PTTG1), also known as securin, a regulator of sister chromatid separation and transition from metaphase to anaphase. This study was conducted to clarify the less-known functions of PTTG1 in the epidermis by the use of keratinocytes cultured under two-dimensional (2D) or three-dimensional (3D) conditions. Forced overexpression of PTTG1 caused upregulation of cyclin B1, cyclin-dependent kinase 1 (CDK1), and c-Myc, resulting in enhanced proliferation and suppression of early differentiation without apparent alterations in terminal differentiation, and the exogenous PTTG1 was downregulated in association with cell cycle exit. In contrast, depletion of PTTG1 caused their downregulation and constrained proliferation with retention of differentiation capacity. These findings suggested that PTTG1 could alter the proliferation status by modulating the expression levels of the other cell cycle regulatory proteins, and excess PTTG1 primarily affects early differentiation of keratinocytes under the stability regulation associated with cell cycle exit.

著者

Takuma AOKI Keisuke SUGIMOTO Hiroshi SUNAHARA Yoko FUJII

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.2, pp.199-202, 2013 (Released:2013-03-01)

参考文献数

17

被引用文献数

8 14

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We report two feline cases of patent ductus arteriosus (PDA) with pulmonary hypertension (PH). The subjects were both intact domestic shorthair cats, a 4-month-old, 2.5 kg male (case 1) and an 8-month-old, 2.12 kg female (case 2). At the first presentation, left-sided congestive heart failure was diagnosed in case 1 and severe aortic stenosis (AS) in case 2. Following surgical ligation of the ductus arteriosus (DA), furosemide therapy was no longer required in case 1, and the severe AS improved to mild status in case 2 perhaps because of reduced volume overload. In case 2, severe hypoxemia was revealed after surgery; however, this normalized within 96 days after surgery.

著者

Žagar Nedjeljka Terasaki Koji Tanaka Hiroshi L.

出版者

American Meteorological Society

雑誌

Monthly weather review (ISSN:00270644)

巻号頁・発行日

vol.140, no.7, pp.2297-2307, 2012-07

被引用文献数

9

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This paper deals with the large-scale inertio-gravity (IG) wave energy in the operational ECMWF analyses in July 2007. Energy percentages of the IG waves obtained from the standard-pressure-level data are compared to those derived from various discretizations of the model-level data. The results show a small albeit systematic increase of the IG energy percentage as the vertical level density increases from the standard-pressure levels toward the model-level density; the small relative change is explained by the sufficient vertical resolution to resolve the large-scale IG waves in the tropics that make the majority of the global IG energy on large scales. A relatively larger increase of the IG energy is obtained when the mesospheric model levels are included; however, the analyses at these levels in July 2007 are less reliable. Furthermore, two numerical methods for the normal-mode function (NMF) decomposition are shown to provide similar results. The decomposition of atmospheric analyses into the NMF series is proposed as a tool to analyze the spatial and temporal variations of the large-scale equatorial waves and their role in global energetics.