著者

Yoo Bin CHOI Geon A KIM Hyun Ju OH Min Jung KIM Young Kwang JO Erif Maha Nugraha SETYAWAN Seok Hee LEE Byeong Chun LEE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0107, (Released:2015-10-19)

被引用文献数

9

---

Somatic cell nuclear transfer is a useful tool to maintain genetic information of animals. The Gyeongju Donggyeong dog is a breed registered as natural monument in Korea. The Unique feature of the Donggyeong dog is its tail, as the Donggyeong dog can be classified as either short tailed or tailless. The aim of this study was to preserve the Donggyeong dog’s unique feature by cloning. Fibroblasts were obtained from a short-tailed Donggyeong dog. In vivo matured oocytes were enucleated, microinjected with a donor cell and fused electrically. Reconstructed embryos were transferred to six recipient dogs. One surrogate became pregnant, and one short-tailed Donggyeong dog was delivered. This study demonstrated that the phenotype of the Donggyeong dog could be conserved by somatic cell nuclear transfer.

著者

Jongsung LEE Sangil SUH Ran CHOI Changbaig HYUN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0065, (Released:2015-08-09)

被引用文献数

11

---

This study evaluated anesthesia quality, degree of analgesia, and cardiorespiratory parameters after intramuscular (IM) injection of a combination of butorphanol (0.1 mg/kg), medetomidine (10 µg/kg), and alfaxalone (1.5 mg/kg) in ten healthy adult Beagle dogs. Rectal temperature (T), heart rate (HR), respiratory rate (fR), arterial pressure, arterial blood gases, and M-mode echocardiographic left ventricular (LV) indices were measured before drug administration and every 10 min thereafter until extubation. Mean duration of anesthesia, recovery, and analgesia were 89 ± 17, 6 ± 1, and 80 ± 12 min. HR, fR, partial pressure of arterial CO2 and O2, arterial pressure, and LV contractility were significantly altered during anesthesia. IM administration of the drug combination provided acceptable anesthesia, but produced substantial cardiorespiratory suppression.

著者

Yasuyuki KANEKO Shidow TORISU Takumi KOBAYASHI Shinya MIZUTANI Nao TSUZUKI Hiroko SONODA Masahiro IKEDA Kiyokazu NAGANOBU

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0169, (Released:2015-08-08)

被引用文献数

1 2

---

Arterial blood gas analysis is an important diagnostic and monitoring tool for respiratory abnormalities. In human medicine, lung complications often occur as a result of liver disease. Although pulmonary complications of liver disease have not been reported in dogs, we have frequently encountered hypoxemia in dogs with liver disorders, especially extrahepatic biliary obstruction. In addition, respiratory disorders account for 20% of perioperative fatalities in dogs. Therefore, in this study, we evaluated the respiratory status in dogs with hepatobiliary disease by arterial blood gas analysis. PaO2 and PaCO2 were measured. Alveolar-arterial oxygen difference (AaDO2), the indicator of gas exchange efficiency, was calculated. Compared to healthy dogs (control group), hepatobiliary disease dogs had significantly lower PaO2 and higher AaDO2. Hypoxemia (PaO2 of ≤80 mmHg) was observed in 28/71 dogs with hepatobiliary disease. AaDO2 was higher (≥30 mmHg) than the control group range (11.6 to 26.4 mmHg) in 32/71 hepatobiliary disease dogs. By classifying type of hepatobiliary disease, dogs with extrahepatic biliary obstruction and chronic hepatitis showed significantly lower PaO2 and higher AaDO2 than in a control group. Dogs with chronic hepatitis also had significantly lower PaCO2. The present study shows that dogs with hepatobiliary disease have respiratory abnormalities more than healthy dogs. Preanesthetic or routine arterial blood gas analysis is likely beneficial to detect the respiratory abnormalities in dogs with hepatobiliary disease, especially extrahepatic biliary obstruction and chronic hepatitis.

著者

Hiroshi UENO Osamu YAMATO Takeshi SUGIURA Moeko KOHYAMA Akira YABUKI Kenjiro MIYOSHI Kazuya MATSUDA Tsuyoshi UCHIDE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0281, (Released:2015-08-03)

被引用文献数

1 3

---

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.

著者

Hassadin BOONSRIROJ Daria Llenaresas MANALO Kazunori KIMITSUKI Taichi SIHMATSU Nozomi SHIWA Harumi SHINOZAKI Yurika TAKAHASHI Naoto TANAKA Satoshi INOUE Chun-Ho PARK

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0308, (Released:2015-08-14)

被引用文献数

3 13

---

Rabies is a zoonotic disease caused by the rabies virus. While the salivary glands are important as exit and propagation sites for the rabies virus, the mechanisms of rabies excretion remain unclear. Here, we investigated the histopathology of the salivary glands of rabid dogs and analyzed the mechanism of excretion into the oral cavity. Mandibular and parotid glands of 22 rabid dogs and three control dogs were used. Mild to moderate non-suppurative sialadenitis was observed in the mandibular glands of 19 of the 22 dogs, characterized by loss of acinar epithelium and infiltration by lymphoplasmacytic cells. Viral antigens were detected in the mucous acinar epithelium, ganglion neurons and myoepithelium. Acinar epithelium and lymphocytes were positive for anti-caspase-3 antibodies and TUNEL staining. In contrast, no notable findings were observed in the ductal epithelial cells and serous demilune. In the parotid gland, the acinar cells, myoepithelium and ductal epithelium all tested negative. These findings confirmed the path through which the rabies virus descends along the facial nerve after proliferation in the brain to reach the ganglion neurons of the mandibular gland, subsequently traveling to the acinar epithelium via the salivary gland myoepithelium. Furthermore, the observation that nerve endings passing through the myoepithelium were absent from the ductal system suggested that viral proliferation and cytotoxicity could not occur there, ensuring that secretions containing the virus are efficiently excreted into the oral cavity.

著者

Huanan WANG Xu YANG Yipeng JIN Shimin PEI Di ZHANG Wen MA Jian HUANG Hengbin QIU Xinke ZHANG Qiuyue JIANG Weidong SUN Hong ZHANG Degui LIN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0484, (Released:2015-07-09)

---

CHIP (Carboxy terminus of Hsc70 Interacting Protein) is an E3 ubiquitin ligase that can induce ubiquitination and degradation of several oncogenic proteins. The expression of CHIP is frequently lower in human breast cancer than in normal breast tissue. However, the expression and role of CHIP in the canine mammary gland tumor (CMGT) remain unclear. We investigated the potential correlation between CHIP expression and mammary gland tumor prognosis in female dogs. CHIP expression was measured in 54 dogs by immunohistochemistry and real-time RT-PCR. CHIP protein expression was significantly correlated with the histopathological diagnosis, outcome of disease, and tumor classification. The transcriptional level of CHIP was significantly higher in normal tissues (P=0.001) and benign tumors (P=0.009) than it in malignant tumors. CHIP protein expression was significantly correlated with the transcriptional level of CHIP (P=0.0102). The log-rank test survival curves indicated that patients with low expression of CHIP had shorter overall periods of survival than those with higher CHIP protein expression (P=0.050). Our data suggest that CHIP may play an important role in the formation and development of CMGTs and serve as a valuable prognostic marker and potential target for genetic therapy.

著者

Kenjiro FUKUSHIMA Reina FUJIWARA Kie YAMAMOTO Hideyuki KANEMOTO Koichi OHNO Masaya TSUBOI Kazuyuki UCHIDA Naoaki MATSUKI Ryohei NISHIMURA Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0077, (Released:2015-06-27)

被引用文献数

1 27

---

Little information is available regarding triple-phase computed tomography (CT) of canine pancreatic insulinoma. A few case reports with small numbers of cases have indicated that hyper-attenuation in the arterial phase was a common finding on multi-phasic CT in dogs with insulinoma. Our purpose was to clarify the characteristic findings of dogs with insulinoma on triple-phase CT. Nine dogs with insuolinoma that underwent triple-phase CT were included in the present study. Attenuation patterns in the arterial phase indicated hypo-attenuation in 4 cases and hyper-attenuation in 2 cases. In the remaining 3 cases, 1 case showed hypo-attenuation and 1 case showed hyper-attenuation in the pancreatic phase, and 1 case presented hyper-attenuation in the later phase. Altogether, 5 cases showed hypo and 4 cases showed hyper-attenuation in at least one phase. The enhancement pattern was homogenous in 7 cases and heterogeneous in 2 cases. Tumor margins were well-defined in 5 cases and ill-defined in 4 cases. Capsule formation was present in 5 cases and absent in 4 cases. In conclusion, it is important to note that hypo-attenuation was as common as hyper-attenuation in dogs with insulinoma in triple-phase CT in at least one phase. Additionally, mass lesions were most conspicuous not only in the arterial phase but in the pancreatic and later phases in some cases. Therefore, it is important to perform triple-phase CT and notice about variable findings for the detection of canine pancreatic insulinoma.

著者

Narong TIPTANAVATTANA Mongkol TECHAKUMPHU Theerawat THARASANIT

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0207, (Released:2015-06-14)

被引用文献数

1 5

---

The efficiency of spermatogonial stem cell (SSC) isolation and culture from pubertal donors is currently poor primarily because of contamination with other testicular cells. This study aimed to purify SSC-like cells using different extracellular matrixes and a discontinuous gradient density. In experiment 1, testes (n=6) were analyzed for histology and SSC-related protein expressions (laminin, SSEA-4, DDX-4 and GFRα-1). After enzymatic digestion, the cell suspension was plated onto either a laminin- or gelatin-coated dish. The number of SSC-like cells was determined at 15, 30 and 60 min of culture (experiment 2). Experiment 3 was performed to test whether or not the additional step of Percoll gradient density centrifugation could really improve purification of SSC-like cells. Testicular histology revealed complete spermatogenesis with laminin expression essentially at the basal lamina of the seminiferous tubules. SSEA-4 and GFRα-1 co-localized with DDX-4 in the spermatogonia. The relative percentage of SSC-like cells, as determined by cells expressing SSEA-4 (59.42 ± 2.18%) and GFRα-1 (42.70 ± 1.28%), revealed that the highest SSC-like cell purity was obtained with the 15-min laminin-coated dish compared with other incubation times and gelatin treatment (P<0.05). Percoll treatment prior to laminin selection (15 min) significantly improved SSC-like cell recovery (91.33 ± 0.14%, P<0.001) and purity (83.82 ± 2.05% for SSEA-4 and 64.39 ± 1.51% for GFRα-1, P<0.05). These attached cells demonstrated a typical SSC-like cell morphology and also expressed POU5F1, RET and ZBTB16 mRNA. In conclusion, double enrichment with Percoll gradient density centrifugation and laminin plating highly enriched the SSC-like cells population.

著者

Shoichi SAKAGUCHI Rie KOIDE Takayuki MIYAZAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0213, (Released:2015-05-31)

被引用文献数

2 17

---

Feline morbillivirus (FmoPV) is an emerging virus in cats, which is associated with tubulointerstitial nephritis. To study the in vitro host range of FmoPV, we inoculated FmoPV strain SS1 to 32 cell lines originated from 13 species and cultured for two weeks, followed by RNA extraction and reverse-transcription-polymerase chain reaction for FmoPV detection. As a result, only cell lines derived from cats and African green monkeys were susceptible to FmoPV. FmoPV infects diverse feline cell lines: epithelial, fibroblastic, lymphoid and glial cells. These results indicate that the receptor (s) for FmoPV are ubiquitously expressed in cats. No infectivity of FmoPV was observed in human cell lines, which suggests least threatening of cross-species transmission of FmoPV from cats to humans.

著者

Mizuki TOMIHARI Akira NISHIHARA Terumasa SHIMADA Masashi YANAGAWA Masafumi MIYOSHI Kazurou MIYAHARA Akihiro OISHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0611, (Released:2015-05-11)

被引用文献数

1 14

---

Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups, the number of lymphocytes in peripheral blood decreased after 2 hr of anesthesia (2 hr), but group P showed significantly less decrease than group I. For T-lymphocyte subsets examined by flowcytometry, the ratio of CD3+, CD4+, and CD8+ lymphocytes in the peripheral blood mononuclear cell (PBMC) of group P at 2 hr also exhibited a high level compared to group I. Moreover, for mRNA expression of cytokines measured by real-time PCR, the IL2 (pro-inflammatory cytokine) of group P showed no decrease like group I. The IL10 (anti-inflammatory cytokine) of group P also showed no increase like group I, while both cytokines maintained nearly the same level until 2 hr. These results suggest that, compared to propofol, isoflurane had more strongly immuno-suppression caused by anesthesia, and propofol itself might have some immuno-protective effects. Thus, TIVA with propofol might benefit immunological support in the perioperative period of dogs.

著者

Rie KOIDE Shoichi SAKAGUCHI Takayuki MIYAZAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0623, (Released:2015-01-26)

被引用文献数

3 15

---

Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence (IF) technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.

著者

Kei KONDOH Ayman ATIBA Kiyoshi NAGASE Shizuko OGAWA Takashi MIWA Teruya KATSUMATA Hiroshi UENO Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0279, (Released:2015-04-04)

被引用文献数

1

---

In the present study, we compare a new carbon dioxide (CO2) absorbent, Yabashi lime® with a conventional CO2 absorbent, Sodasorb® as a control CO2 absorbent for Compound A (CA) and Carbon monoxide (CO) productions. Four dogs were anesthetized with sevoflurane. Each dog was anesthetized with four preparations, Yabashi lime® with high or low-flow rate of oxygen and control CO2 absorbent with high or low-flow rate. CA and CO concentrations in the anesthetic circuit, canister temperature, and carbooxyhemoglobin (COHb) concentration in the blood were measured. Yabashi lime® did not produce CA. Control CO2 absorbent generated CA and its concentration was significantly higher in low-flow rate than a high-flow rate. CO was generated only in low-flow rate groups, but there was no significance between Yabashi lime® groups and control CO2 absorbent groups. However, the CO concentration in the circuit could not be detected (≤5 ppm) and no change was found in COHb level. Canister temperature was significantly higher in low-flow rate groups than high-flow rate groups. Furthermore, in low-flow rate groups, the lower layer of canister temperature in control CO2 absorbent group was significantly higher than Yabashi lime® group. CA and CO productions are thought to be related to the composition of CO2 absorbent, flow rate, and canister temperature. Though CO concentration is equal, it might be safer to use Yabashi lime® with sevoflurane anesthesia in dogs than conventional CO2 absorbent at the point of CA production.

著者

平尾 秀博 井上 知紀 星 克一郎 小林 正行 島村 俊介 清水 美希 田中 綾 高島 一昭 森 有一 野一色 泰晴 山根 義久

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.67, no.4, pp.357-362, 2005-04-25

被引用文献数

2

---

新型の弁付き導管を犬大動脈弁を用いて作成した.生体代替弁はグルタールアルデヒドとエポキシ化合物(Denacol-EX313/810)により固定した.超極細ポリエステル繊維製人工血管(直径10mm, 全長20mm)を使用した.犬4頭に左心室-大動脈間弁付き導管(AAVC)移植術と大動脈バンディングを行いバイパス群とした.もう4頭にはAAVC移植術は行わず大動脈パンディングのみを行いコントロール群とした.心臓カテーテル検査と心血管造影検査を術後2週間, 6カ月に行い, 血行動態を評価した.左室収縮期圧, 左室拡張終期圧, 左室-大動脈間圧較差それぞれにおいて2群間に有意な差(p<0.01)がみられた.左室心血管造影検査でバイパス群の全頭において弁付き導管の開存が確認された.心臓エコー検査を術前, 術後2, 4, 6カ月に行った.コントロール群では圧負荷による心筋の求心性肥大がみられ, 一方バイパス群では左室の遠心性肥大がみられ, AAVCにより左室圧負荷の軽減が維持されていることが示唆された.

著者

李 振泰 塔 娜 渡来 仁 柿谷 均 小沼 操 趙 丹丹 保田 立二

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.59, no.3, pp.169-174, 1997-03-25

被引用文献数

3

---

陽性荷電リポソームが, ウシ白血病ウイルス(BLV)感染細胞への毒素遺伝子の導入に応用可能かどうかを調べた. 陽性荷電リポソームは, N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride(TMAG), dioleoyl phosphatidylethanolamine(DOPE), dilauroyl phosphatidylcholine(DLPC)(モル比1 : 2 : 2)から作製し, 遺伝子を封入させた. ルシフェラーゼアッセイにより, 陽性荷電リポソーム(TMAGリポソーム)によるBLV感染細胞(FLK/BLV細胞)への遺伝子導入効率を調べたところ, TMAGリポソームの遺伝子導入効率は, ホスファチジルセリン(PS)から作製されたリポソームに比べ高い導入効率を示した. さらに, ルシファラーゼ遺伝子とともにプロモーター活性を持つBLVのLTRの下流にジフテリア毒素遺伝子を挿入したプラスミドDNA(pLTR-DT)をTMAGリポソームによりFLK/BLV細胞にco-transfectionし, ルシフェラーゼ遺伝子によるルシフェラーゼ活性が, TMAGリポソームにより導入されたpLTR-DTにより, どの程度阻止されるかを調べることにより, pLTR-DT封入TMAGリポソームの, BLV感染細胞に対する殺傷効果を検討した. その結果, ルシフェラーゼ活性は, pLTR-DTを導入することによりdose-dependentに抑制された. また, pLTR-DTをFLK/BLV細胞に複数回導入すると, FLK/BLV細胞の増殖が顕著に抑制された. さらに, pLTR-DT封入TMAGリポソームを血清あるいは核酸分解酵素と反応させたところ, TMAGリポソームに封入された毒素遺伝子は分解されなかった. これらのことから, 陽性荷電(TMAG)リポソームはBLV感染細胞への遺伝子導入法として優れており, 毒素遺伝子封入陽性荷電リポソームによる, BLV感染細胞の遺伝子治療の可能性が示唆された.

著者

佐藤 宏 稲葉 孝志 井濱 康 神谷 晴夫

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.61, no.9, pp.1023-1026, 1999-09-25

参考文献数

16

被引用文献数

6 31

---

1997-1998年の冬季に, 本邦東北地方北西部に生息する60頭の野生肉食類について寄生虫学的検討を行った. これらは, 青森・秋田両県下で捕獲ないしは交通事故死したホンドキッネ7頭, ホンドタヌキ20頭, ホンドテン29頭, ホンドイタチ2頭, 二ホンイイズナおよび二ホンアナグマ各1頭であった. キツネおよびタヌキでは, 回虫(それぞれToxocara canisおよびT. tanuki), 鉤虫(Ancylostoma kusimaenseおよびArthrostoma miyazakiense), Molineus sp.が高率に回収された. テンでは, 胃のAonchothecaputorii, 膵管のConcinnumten, 小腸のMo1ineus sp.とEuryhelmis costaricerlsisが高率に寄生していた. 従来から分布の知られていた寄生虫種に加えて, この地方あるいは本邦での分布が知られていなかった次のような寄生嬬虫種が確認された. すなわち, キツネからのTaenia polyacantha,タヌキからのPygidiopsis summa, テンからのEucoleus aerophilus, A. putorii, Soboliphyme baturiniである.

著者

Hiroto MAEDA Kazuyuki SOGAWA Kazuko SAKAGUCHI Saori ABE Wataru SAGIZAKA Shunsuke MOCHIZUKI Waka HORIE Toshifumi WATANABE Yui SHIBATA Mamoru SATOH Akihiro SANDA Fumio NOMURA Jun SUZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0427, (Released:2015-03-28)

被引用文献数

2 20

---

Feline renal diseases are increasingly noted in veterinary practice. It is important to diagnose and identify the pathological basis of renal dysfunction accurately at an early stage, but there are only a few reports on this area in clinical veterinary medicine. We investigated the efficacy of measurement of urinary albumin (u-Alb) and urinary transferrin (u-Tf) for early diagnosis using 5-µl urine samples collected noninvasively by catheterization from normal (IRIS stage I) cats and cats with stage I chronic kidney disease (CKD). The u-Alb levels in normal and stage I CKD cats were 6.0 ± 4.5 and 11.2 ± 8.4 mg/dl, respectively, and the u-Tf levels were 0.09 ± 0.42 and 0.52 ± 0.79 mg/dl, respectively. Based on ROC curve analysis, the sensitivity and specificity of u-Alb and u-Tf were higher than those of the currently used biomarker, the plasma creatinine level. The sensitivity of u-Alb was higher than that of u-Tf, whereas the specificity of u-Tf was higher than that of u-Alb. The validity of the threshold albumin level (20 mg/dl) was confirmed by measurements using SDS-PAGE. Since leakage of u-Tf in urine precedes leakage of u-Alb, inclusion of u-Tf in biochemistry tests may be appropriate for IRIS staging as a diagnostic marker of early diagnosis of renal disorder in cats.

著者

淺野 玄 的場 洋平 池田 透 鈴木 正嗣 浅川 満彦 大泰司 紀之

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.65, no.3, pp.s・ix, 369-373, 2003-03-25

被引用文献数

6 28

---

1999-2000年に北海道で捕獲されたメスのアライグマ(Procyon lolor)242個体について,胎盤痕または胎子を分析して繁殖学的特性を調査した.捕獲個体の齢構成は,0歳69個体(29%), 1歳71個体(29%)および2歳以上102個体(42%)であった.1歳の平均妊娠率は66%で,2歳以上の平均妊娠率96%と比べて有意に低値であった.一腹産子数は1頭から7頭で,平均産子数は1歳で3.6頭,2歳以上では3.9頭であった.平均産子数には1歳と2歳以上とで有意差は認められなかった.北海道の移入アライグマの繁殖期は,2月が交配のピークで3月から5月が出産期であると推定された.しかし,7月に2頭の妊娠個体が確認されたことから,夏期にも繁殖することが明らかとなった.北海道のアライグマの繁殖ポテンシャルは北米における報告と同程度であり,個体数増加の主要因であると考えられた.夏期の箱罠捕獲における0歳獣の捕獲圧は,1歳以上の個体と比較して相対的に低いことが示唆された.移入アライグマの個体数を減少させるためには,個体数が多い0歳獣の捕獲圧を高める必要があり,効率的な捕獲方法の検討が求められる.

著者

山田 学 中村 菊保 西道(坂中) 寿子 朝岡 愛 山川 稔 鮫島 俊哉 本部 真樹 廣田 好和

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.66, no.2, pp.137-142, 2004-02-25

被引用文献数

3 16

---

これまでにカブトムシ(Allomyrina dichotoma)の生産する免疫関連蛋白質を分離精製し,その精製した蛋白質の部分配列が抗微生物作用を持つことを明らかにし,さらにこの抗微生物蛋白質から得られた改変ペプチドは薬剤針性病原菌に対しても抗菌作用を持つことを示唆した.そこで,新たに精製された2種類の昆虫の抗微生物蛋白質由来の改変ペプチド(RLYLRIGRR-NH_2 : ペプチドA ; RLRLRIGRR-NH_2 : ペプチドB)の薬剤耐性大腸菌への効果を,マウスを用いて病理学的に検討した.ペプチドA接種群では,死亡率,解剖所見,組織所見ともに,ペプチド未接種群との有意な差は認められなかった.一方,ペプチドB接種群では,ペプチド未接種群と比べて,死亡率は低下し,解剖所見,組織所見においても病変は著しく軽度に認められた.ペプチドA,B共にマウスに対して組織毒性を示さなかった.ペプチドBは薬剤耐性細菌に対して有効であることが実験動物を用いた試験においても示唆された.

著者

Jun TAMURA Tomohito ISHIZUKA Sho FUKUI Norihiko OYAMA Kodai KAWASE Takaharu ITAMI Kenjiro MIYOSHI Tadashi SANO Kirby PASLOSKE Kazuto YAMASHITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0200, (Released:2015-03-19)

被引用文献数

48

---

The sedative effects of intramuscular (IM) alfaxalone in 2-hydroxypropyl-beta-cyclodextrin (alfaxalone-HPCD) were evaluated in cats. The cats were treated with alfaxalone-HPCD in five occasions with a minimum 14-day interval between treatments: an IM injection of 1.0 mg/kg (IM1), 2.5 mg/kg (IM2.5), 5 mg/kg (IM5) or 10 mg/kg (IM10), or an intravenous injection of 5 mg/kg (IV5). The sedative effects were evaluated subjectively using a composite measurement scoring system (a maximum score of 16). Cardio-respiratory variables were measured non-invasively. The median sedation scores peaked at 10 min (score 9), 15 min (score 14), 10 min (score 16), 10 to 20 min (score 16) and 2 to 5 min (score 16) after the IM1, IM2.5, IM5, IM10 and IV5 treatment, respectively. The IM5 treatment produced longer lasting sedation, compared to the IV5 treatment. Durations of maintenance of lateral recumbency after the IM10 treatment (115 ± 22 min) were longer than those after the IM2.5 (40 ± 15 min), IM5 (76 ± 21 min) and IV5 treatments (50 ± 5 min). Cardio-respiratory variables remained within clinically acceptable ranges, except for each one cat that showed hypotension (<60 mmHg) after the IM10 and IV5 treatments. Tremors, ataxia and opisthotonus-like posture were observed during the early recovery period after the IM2.5, IM5, IM10 and IV5 treatments. In conclusion, IM alfaxalone-HPCD produced dose-dependent and clinically relevant sedative effect at 2.5 to 10 mg/kg in healthy cats. Hypotension may occur at higher IM doses of alfaxalone-HPCD.

著者

Seishiro CHIKAZAWA Yasutomo HORI Fumio HOSHI Kazutaka KANAI Naoyuki ITO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0314, (Released:2015-02-19)

被引用文献数

2

---

In the present study, we examined the relationship between serum ferritin concentration before treatment and survival time in dogs with multicentric lymphoma. Eighteen dogs with multicentric lymphoma were enrolled in the study. When the dogs were classified into high and low ferritin groups on the basis of their serum ferritin concentration (3,000 ng/ml cut-off value), the median survival time of dogs with high concentrations (≥3,000 ng/ml, n=7) was 40 days, whereas it was 360 days among dogs with low concentrations (<3,000 ng/ml, n=11). This difference was statistically significant (P=0.001). This finding suggests that the initial high level of serum ferritin indicates short survival time in dogs with multicentric lymphoma. Large-scale research is necessary to confirm this finding.