著者

趙 伯陽 桜井 陽平 柴田 清澄 吉川 史隆 友田 豊 水上 元

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.21, no.1, pp.42-47, 2014

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ナスのヘタは尋常性疣贅(イボ)の治療に伝承的に用いられている。また、ナスのガク片部のエタノール抽出物が尖形コンジローマの治療に有効であったことが報告されている。そこで、ナスのエタノール抽出物の各種ヒト腫瘍細胞株に対する細胞致死活性を調べたところ、ヒト卵巣ガン由来の細胞株であるHRAに対して細胞致死活性を示し、その効果はガク片部からの抽出物が可食部からの抽出物よりも強かった。この抽出物から活性に基づく分画を行うことにより、2つの細胞致死活性成分9-oxo-(10E, 12Z)-octadecadienoic acid (9-EZ-KODE)および9-oxo-(10E, 12E)-octadecadienoic acid (9-EE-KODE)を単離・同定した。9-EE-KODEは、9-EZ-KODEの約10倍高い細胞致死活性を示した。また、9-EE-KODEの細胞致死活性を各種細胞株を用いて比較したところ、HRAに対して他の細胞株よりも約5倍強い活性を示した。ナスのガク片部の9-EE-KODE含量は可食部よりも高かった。

著者

金 海麗 田中 隆 河野 功 藤岡 稔大 吉田 都 石丸 幹二

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.13, no.3, pp.136-140, 2006-12-30

参考文献数

23

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Shoot, adventitious root and hairy root cultures of Solidago altissima L. were established. Chlorogenic acid and 3, 5-dicaffeoylquinic acid were isolated from the hairy root cultures. 3, 5-dicaffeoylquinic acid was the major secondary metabolites in various tissue cultures of this plant. These polyphenol compounds were easily isolated as the polyphenol-soybean protein complex from the extract of this plant. Solidago altissima L., producing polyphenol compounds with the high concentrations, seemed to have a potential to be as a new resource for functional compounds.

著者

冨岡 華代 高津 万結香 巽 美智 長谷川 絢 北田 善三

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.21, no.1, pp.15-20, 2014

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A method has been developed for determining amygdalin (AM) and its degradation products, benzyl alcohol (BeOH), benzaldehyde (BAL) and benzoic acid (BA) in Chinese quince fruit utilizing high performance liquid chromatography (HPLC). A sample was extracted with 0.05 mol/L citric acid solution (pH2.2) and cleaned in an InertSep C18 cartridge and an InertSep NH₂ cartridge. The HPLC separation was performed on an Inertsil ODS-4 column using acetonitrile-0.01 mol/L phosphate buffer (pH2.0) (15:85) in the mobile phase. A UV monitor was used for detection. The effects during growth period on the amount of 4 components of sarcocarp and seed of Chinese quince was examined. AM content in seed increased proportionally each month. This increase was especially significant in seed retrieved in November. AM was detected in commercially available Chinese quince in the range of 0.03-0.06 mg/g sarcocarp, 0.21-1.75 mg/g seed. BA was not detected in any samples.

著者

矢野 潤 上地 理恵 雨坪 知音 義平 邦利

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.8, no.3, pp.155-160, 2002-01-24

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これまで測定例がほとんど報告されていないキサンテン系の食用赤色色素(食用赤色3号、食用赤色104号、食用赤色105号、食用赤色106号)のサイクリックボルタンモグラムを測定したところ、すべてのサイクリックボルタンモグラムにおいて、明らかに食用色素のものと考えられる酸化電流ピークが観測された。酸化電流ピーク電位は、食用赤色3号が+0.725V、食用赤色104号が+0.810V、食用赤色105号が+0.801V、食用赤色106号が+1.010Vであった。したがって、もしこれらが還元作用を有するならば、その還元能力は食用赤色3号>食用赤色105号>食用赤色104号>食用赤色106号の順で高いことが予想された。他方、還元電流ピークは還元側の電位を-0.5Vまで走査しても、ほとんど観測されなかった。このことはこれらの食用色素から酸化されて精製したカチオンラジカルあるいはジカチオンが不安定で、水あるいは色素自身と反応し、レドックス不活性種と変化したために、もはや還元電位に保持されてももとの色素には戻れなくなったためと考えられた。またそれぞれの酸化電流ピーク電位の差異は芳香環置換されている官能基の電子誘起効果のためと示唆された。食用赤色3号、食用赤色104号、食用赤色105号、食用赤色106号をそれぞれ+1.1Vの定電位で酸化したところ、電解酸化によって色素の濃度は減少したが、所定の電解時間および電解終了時に測定したサイクリックボルタンモグラムには色素の酸化電流以外には他のレドックス電流は観測されなかったため、その電解酸化生成物はレドックス活性な化合物ではなかった。電解前後の電解溶液の可視光吸収スペクトルを測定した結果、両者においてあまり変化は見られなかったが、電解終了時の電極表面上には赤色の固体生成物が認められた。まだそれらの分子構造は決定できていないが、おそらく色素が多量化したものと推察された。抗酸化性については、典型的な抗酸化剤であるBHAよりは低いが、酸素の還元電位より酸化電位が低い食用赤色3号、食用赤色104号、食用赤色105号は抗酸化剤として機能することが予想された。

著者

北元 憲利 田中 智之 加藤 陽二 辻 啓介

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.7, no.2, pp.86-92, 2000-10-28

参考文献数

13

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こんにゃくは、水酸化カルシウム溶液に浸されたアルカリ性を呈する食品であるが、腸管出血性大腸菌O157との関わりを検討した報告はない。そこで、本研究では、こんにゃく液中におけるO157の生存状況と、こんにゃく液の抗菌効果(静菌作用あるいは殺菌作用)について検討した。こんにゃく液はいずれもpH12前後であり、この高いpH域では、O157は反応させるだけで検出限界までに激減することがわかった。初発菌数が多い場合でも1日の反応(保存)でほとんどが死滅した。また、この抗菌作用は大腸菌の株による違いや温度差には影響されなかった。一方、食中毒菌であるサルモネラ菌、腸炎ビブリオ菌および黄色ブドウ球菌についても同様に比較検討したところ、こんにゃくの液の抗菌効果は、O157と比べると同等かあるいはやや弱いようであった。しかし、これらの食中毒菌もこんにゃく液中では時間とともに減少し、2〜3日後ではほとんどが死滅することが判明した。さらに、市販こんにゃく液中の細菌の存在有無を検討したが、調べた限り、開封直後のこんにゃく液中には菌は検出されなかった。以上の結果から、こんにゃく液は衛生学的に安全性の高いことが証明され、静菌作用(菌の増殖抑制・阻害作用)というよりは、殺菌作用(生菌数の低減化作用)をもつことが明らかとなった。こんにゃくのような高アルカリ性呈示食品は、少数の生菌数の摂取でも発症するような腸管出血性大腸菌O157などの感染症防止に対しても、大きな役割を果たしているものと考えられる。

著者

馬場 二夫 森田 茂 杉田 たき子 石綿 肇

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.2, no.1, pp.51-53, 1995-10-01

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陶磁器製の食器からの鉛とカドミウムの溶出濃度の調査と,アメリカ食品医薬局(FDA)により提案された鉛の規制値案への適合率を調べる目的で,以下の実験を行った.溶出条件は,わが国の現行試験法(国際標準化機構,ISO,でもほぼ同一条件)である4%酢酸を用いて25℃で24時間の条件で実験をおこなった.鉛およびカドミウムの測定はICPによった.実験に用いた83種の市販試料のうち,鉛の溶出は44試料で,また,カドミウムの溶出は26試料で認められた.試料内側(食品と接触する面)から溶出した鉛の平均値±S.D.,最高値,最低値はそれぞれ0.48±1.32, 8.78, 0.00 μg/cm^2であった.カドミウムでは,それぞれ0.01±0.03, 0.25, 0.00 μg/cm^2であった.鉛の溶出は,ゆう薬のある54試料中16試料で,また,ゆう薬の無い29試料全てで認められた.平均値は,前者で0.04±0.18 μg/cm^2,後者では1.28±2.00 μg/cm^2であった.カドミウムの溶出は,ゆう薬のある54試料中9試料で,また,ゆう薬の無い29試料中17試料で認められた.平均値は,前者で0.00±0.01 μg/cm^2,後者では0.03±0.05 μg/cm^2であった.本実験に用いた83試料中1試料で規格値(抽出液中5 ppm)を超える7.37 ppmの鉛が溶出した.これらの結果は,約20年前に同一溶出条件の下で行われた調査結果と比べ,1/10またはそれ以下であった.以上の結果を,わが国の現行規格(ISOの規格案も同じ)に従って分類してTable 3に示した.違反事例の1試料を除き,最高値でも規格値よりかなり低濃度であった.また,深さが2.5cmを超える73試料のうち57試料(78%)では鉛の溶出がFDAの規格案である0.1 ppm以下であった。

著者

木村 雅行 池田 雅和 柴田 英之 池田 祥子 松本 圭介

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.11, no.2, pp.67-74, 2004-09-28 (Released:2017-12-01)

参考文献数

13

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To estimate the laxative threshold (LT) and 50% effective dose (ED50) for diarrhea of a galactooligosaccharide (GOS) syrup (43% GOS ; 9.5% lactose ; 18% glucose ; 6.3% galactose ; 24% water), we asked 24 healthy subjects (12 men and 12 women ; age, 20 to 58 years ; weight, 39.0 to 96.0 kg) to ingest 190mL of aqueous solutions containing 5 different amounts (11.7,23.3,46.7, 70.0, 93.3g) of the syrup once every week from a low content to high one. Of the 24 subjects, 16 had diarrhea after ingestion of any test beverage. We estimeted the LT and ED50 of the GOS syrup by using a regression equation between the minimal dose levels (g/kg body weght) of the syrup caused diarrhea and the cumulative incidence of diarrhea. For the entire population, the estimated LT was 0.68 g/kg (0.30 g/kg as GOS) and the ED50 was 1.39 g/kg (0.60 g/kg as GOS). For men, the LT and ED50 were 0.65 g/kg and 1.13 g/kg, respectively, as compared with 0.90 g/kg and 1.50 g/kg for women. An increased frequency of bowel movements seemed to increase the incidence of diarrhea. The other abdominal symptoms reported were borborygmus (88%), flatus (58%), and abdominal distention (46%), all of which were transient, as was diarrhea. We also examined the safety of ingesting daily near amount of LT of the GOS syrup (15 g/person daily as GOS) for 2 weeks in 20 healthy adults. Although this dose increased the occurrence of abdominal gas in these subjects, the prevalence of diarrhea was unchanged as compared with that for the placebo beverage. These results show that the GOS syrup is safe even in repeated intake for near amount of LT.

著者

生野 彰宏 南川 佑太 竹林 慎一郎 奥村 克純

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.28, no.1, pp.23-32, 2021 (Released:2021-04-20)

参考文献数

47

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Many studies have shown that folate deficiency induces DNA hypomethylation and DNA damage. DNA hypomethylation is thought to occur during DNA replication through deficiency of the DNA methylation donor synthesized using folate. However, the relationship between DNA hypomethylation and DNA damage caused by folate deficiency is not well understood. Here, we analyzed DNA damage caused by folate deficiency during the cell cycle S phase in which DNA hypomethylation occurs. Using HeLa cells, we first detected accumulation of intranuclear γ-H2A.X, a DNA double strand break marker, by immunofluorescent staining. We found that DNA damage occurs primarily during S phase, and that folate deficiency enhanced S phase-dependent DNA damage. We also found that folate deficiency caused slowing of the DNA replication fork and a delay in the onset of middle-late S phase. Detailed analyses of DNA damage in S phase progression suggested that the folate deficiency-induced DNA damage increased in all stages of S phase, particularly in middle-late S phase cells. To examine the relationship between DNA damage and DNA hypomethylation, we analyzed the methylation state of the promoter region of the human LINE1 repetitive sequence, where heterochromatin is formed and replicated during middle to late S phase. We found that DNA methylation decreased by about 10% under the folate deficiency condition. Taken together, our results suggest that DNA hypomethylation is associated with DNA damage caused by folate deficiency.

著者

中西 希代子 宮本 文夫 橋本 博之 本郷 猛 林 千恵子 石井 俊靖

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.20, no.1, pp.37-41, 2013-04-22 (Released:2017-01-27)

参考文献数

8

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Daily intake of glyphosate in 2010 and 2011 at Chiba prefecture was estimated using total diet samples prepared according to the market basket method. One hundred eighty six and 175 kinds of foods were purchased from supermarket at Chiba in December 2010 and December 2011, respectively. The purchased foods were divided into 14 food groups as total diet samples, and contents of glyphosate in those groups was analysed by high-performance liquid chromatography with fluorescence (HPLC-FL). Glyphosate was detected from second food group (Cereals and potatoes) and 13th food group (Seasonings and spices, other foods) among 14 food groups. Estimated daily intake of glyphosate in all food groups were 24.2μg/day in 2010, and 17.6μg/day in 2011. These estimated daily intake were accounted for 0.064% in 2010, and 0.047% in 2011 of the ADI assuming a body weight of 50 kg. The foods contribute to glyphosate detected from second food group were breads, fu, boiled noodle, macaroni, and that in 13th food group was soy sauce. Glyphosate detected by HPLC-FL method from above two food groups and those foods was identified by liquid chromatography with tandem mass spectrometry. Above these foods were all contained flour. Therefore, it seems to be high possibility that detected glyphosate is originated from flour.

著者

浅野 未来 西塔 正孝 永井 毅

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.29, no.1, pp.52-60, 2022-04-27 (Released:2022-04-27)

参考文献数

36

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The aim of this study was to clarify the contents of functional components of banana jam prepared using fully ripened banana fruits and to compare the functional properties of banana jam and commercially available (CA) banana jams. The contents of β-carotene and flavonoids of prepared jam were remarkably high when compared with those of CA banana jams. The jam showed high scavenging activities against superoxide anion radicals and DPPH radicals. These activities and hydroxyl radical scavenging activity of the jam were on the same level as those of fully ripened banana fruits, suggesting the concentration of the functional components such as phenols by heat treatment. In addition, the jam exhibited angiotensin I-converting enzyme and hyaluronidase inhibitory activities. Therefore, these findings provide useful information not only for the consumers but also jams and its related industries, and a new market for jams may be achieved.

著者

大井 和裕 義平 邦利 荒木 和美 落合 爲一 中野 昭夫 佐竹 元吉 今吉 有理子 岩渕 久克

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.18, no.3, pp.174-182, 2011-12-15 (Released:2017-01-27)

参考文献数

15

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Five samples of lavenders including four different varieties were collected in Hokkaido Furano area from five different populations. The flowering tops of the samples were soaked into dichloromethane and the extracts were analyzed using GC/MS. Fifty four peaks were observed and 45 of them were identified or their reasonable structures were estimated. Most abundant components common to all samples were linalyl acetate (1), linalool (2), lavandulyl acetate (3), coumarin (4), (E)-β-farnesene (5), 7-metheoxy coumarin (6). Statistical analyses (a clustering analysis, a principal component analysis) were performed on the abundance of the 54 components. The results were as follows: (1) Chemical profiles of the samples which belong to the same variety collected at the same site were essentially the same regardless their collecting time of the day. (2) The difference in the variety resulted in different chemical profile.

著者

三枝 隆裕 藤尾 雄策

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.5, no.2, pp.230-235, 1998

参考文献数

21

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ケフィラン水溶液およびケフィランを無水コハク酸を用いアシル化して得たコハク化ケフィラン水溶液について、特性を比較した結果、ケフィランは低温保存またはエタノールの添加によりゲル化することが認められたが、コハク化ケフィランは、溶液状態での安定性に優れ、高濃度のアルコール類中で長期に渡り保存しても、透明性を保ちゲル化することのない特性を持っていることが確認された。また、コハク化ケフィラン水溶液には、ヒアルロン酸ナトリウム水溶液と同等以上のヒト皮膚への高い保湿効果が認められた。現在、著者らは、コハク化ケフィランを化粧品原料として実際に利用している。さらに、コハク化ケフィランは、その溶液としての優れた安定性から食品、医薬品への工業的な利用が充分可能であると判断された。

著者

木戸 和貴子 吉川 友佳子 中村 衣里 橋本 ゆかり 戸根 瑛美 松浦 寿喜

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.19, no.3, pp.185-190, 2012-12-21 (Released:2017-01-27)

参考文献数

11

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The inhibitory effects against hyperglycemia of "alleged health foods" containing mulberry leaves as an ingredient were compared using an experimental model of digestion and absorption in rats. Rats, after gastric and portal venous catheterization, were continuously fed an aqueous sucrose solution by intragastric administration, and portal venous glucose concentrations were maintained at a constant level. The rats were administered either five types of mulberry leaf tea or three types of mulberry leaf granules, and the time period during which the portal venous glucose concentration showed levels significantly lower than baseline, i.e., the duration of the inhibitory effect on hyperglycemia, was compared. The results showed that although all of these health foods demonstrated an inhibitory effect against hyperglycemia, a comparison of the efficacy based on the duration of this inhibitory effect revealed large disparities among the products, of a magnitude of 17-fold among the mulberry leaf teas and 11-fold among the granular mulberry leaf products. This discrepancy in the efficacy of these types of products could not be discerned by analogy based on the label or the ingredients described on the product. Although alleged health foods are ordinary foods, and efficacy is not demanded, a uniform level of quality assurance is necessary, given that the general consumer purchases these products in expectation of a certain level of effect.

著者

坂元 史歩 佐藤 恭子 米谷 民雄

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.3, no.2, pp.136-140, 1997

参考文献数

7

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The components in a commercial natural food additive "lemon peel extract" and the acetone extract of a commercial lemon peel were studies using high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). HPLC chromatograms for both preparations were almost the same. The peaks corresponding to citral (cis- and trans-forms) were observed as the main peaks in the HPLC and LC/MS chromatograms; however, the peak corresponding to geraniol was not detected. In Japan, four synthetic antimolding agents (o-phenylphenol, diphenyl, thiabendazol and imazalil) are permitted for lemon. By comparison of the retention times and the photodiode-array sepctrum with those of four authentic samples in HPLC, it was suggested that o-phenylphenol was contained in the commercial "lemon peel extract". The quasi molecular ion peak of o-phenylphenol was detected by LC/MS using the atmospheric pressure chemical ionization (APCI) method in the negative mode, and the mass spectrum of the compound that was thought to be o-phenylphenol, was identical to that of the authentic sample. Nevertheless, the amount was so low that it could not contribute to the antimolding activity of the commercial "lemon peel extract". Three other synthetic antimolding agents were not detected in the commercial "lemon peel extract".

著者

俣野 和夫 細野 恭平 藤井 正美

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.1, no.1, pp.68-72, 1995-03-28 (Released:2017-12-01)

参考文献数

10

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CO2 is a food additive that has been listed in "THE JAPANESE STANDARDS FOR FOOD ADDITIVES" since its first edition. According to the questionnaire research conducted by the Ministry of Health and Welfare regarding domestic and imported CO2, a total of 120,000 tons of food grade CO2 from 25 companies was shipped in Japan during 1993. Based on this figure, I think it is necessary to examine how muchCO2 is currently used and to calculate the per capita and per diem Japanese intake, as one of the basic food additives. The atmosphere is composed of 0.035% CO2. Also, various alcohlic beverages such as beer and foamy liqueurs contain CO2, which is produced during the fermentation and manufacturing process. It is a basic component of food and drink. Therefore, we must inspect the various aspects of CO2 when we examine its daily intake. In studying the use of additives which are regarded as company confidential know-how, it is a general procedure to conduct a hearing to determine the production statistics of the food in question. From these studies, we obtained the following estimated breakdown of CO2 that was shipped as food additive: 70,000 to 75,000 tons for airtight rooms and pouches to prevent the oxidation of foods. In the aforementioned types of use, CO2 dissipates immediately after it serves its purpose and is irrelevant to human intake. Also, the quality of food grade CO2 is high and it is often used in other fields such as medicine, research, and foam plastics. The total for these uses is estimated to be approximately 10,000 tons. The human intake of CO2 is mainly attributed to carbonated drinks. According to a industry source, 18,115 tons of CO2 is used in carbonated drinks and 20% of the gas dissipates when the containers are opened. In order to estimate the accumulated amount of CO2 in food, we assumed the following figures for the calculation purpose: 1,200 tons for the CO2 content of beer; 876 tons of CO2 content of canned "chuhai" (Japanese distilled spirit) and foamy liqueurs; and the 20% CO2 loss when the containers are opened. Based on the above assumption, we estimated the per capita and per diem intake of CO2 as food additive in Japan to be 373mg based on Japan's 1993 population. We used only those 20 years of age and older because of prohibition of minors from drinking. As for CO2 as a food component, beer is the largest contribution. Based on the amount of beer and the shipped CO2 content, we estimated the per diem and per capita figure of 943mg. The daily consumption of CO2 is 12,040mg, which is more than ten times larger than the aforementioned intake from foods. This is because we ihnale air that contains 0.035% CO2. As for the respiratory amount, we assumed the per diem and per capita figure of 17.5m3 as an average of man and woman. From this viewpoint, we found that the per capita and per diem CO2 intake of a Japanese is 13,356mg in total. However, this figure is extremely small in comparison to approximately 900g of CO2 that is contained in human expiration. For your reference, 456 tons of sodium dicarbonate, 88.5 tons of magnesium carbonate, and 38.6 tons of calcium carbonate were shipped in 1990 in Japan as medical and pharmaceutical products. They are used as antacid and produce CO2 in human bodies. The produced amounts of CO2 are 238, 22.3, and 17 tons respectively with the total of 277 tons. If we assume that about 70% of the prescribed doses are taken, it amounts to 194 tons and we can obtain the per capita and per diem figure of 4.3mg.

著者

多田 敦子 杉本 直樹 古庄 紀子 石附 京子 佐藤 恭子 山崎 壮 棚元 憲一

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.16, no.2, pp.92-96, 2009-08-24 (Released:2017-01-27)

参考文献数

8

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Ozokerite, a natural gum base used as a food additive, is described as a purified wax substance found in veins of wax shale and is composed mainly of C29-C53 hydrocarbons, in the Notice (1996) relating to existing food additives in Japan. In order to evaluate the quality of commercially available ozokerite, we have analyzed the constituents. GC/MS analysis of ozokerite showed that the main components were saturated C22-C38 hydrocarbons, while the minor components were saturated C39-C58 hydrocarbons. The range of carbon numbers observed in the main saturated hydrocarbons was lower than those referred to in the Notice. The total concentration of the main saturated C22-C38 hydrocarbons was found, by GC/FID analysis, to be 81%.

著者

辻 威彦 籠谷 和弘 梅本 善明 松尾 雄志

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.27, no.2, pp.60-66, 2020 (Released:2020-08-31)

参考文献数

18

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Ginger (Zingiber officinale) rhizomes have been reported to exhibit multiple efficacies on human health. In this paper, we found that n-hexane extract from ginger rhizomes (ginger oil) exhibited the inhibiting abilities against monoamine oxidase A (MAO-A) and B (MAO-B), which catalyze the oxidative deamination of monoamine neurotransmitters, by using an in vitro luminescencebased method. To characterize the compounds responsible for the inhibiting abilities against MAO-A and MAO-B, all fractions from reversed-phase chromatographies were subjected to the luminescence assay. As a result, we isolated five compounds from ginger oil by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The half-maximal inhibitory concentration (IC50) measurement using purified compounds revealed that (ⅰ) 6-, 8-, and 10-gingerols had strong inhibiting ability against MAO-A, whereas they showed weak inhibiting ability against MAO-B. (ⅱ) IC50 values of 6-, 8-, and 10-gingerols against MAO-A were calculated to be 175.7±11.0 μM, 54.6±2.0 μM, and 113.4±4.7 μM, respectively. (ⅲ) Furthermore, we revealed that 1-dehydro- [6]-gingerdione (compound 1) and diacetoxy-[6]-gingerdiol (compound 2) were contained in the ginger oil. (ⅳ) The compound 1 showed strong and selective inhibiting ability against MAO-B with IC50 of 6.77±0.45 μM, which was 10 times stronger than that against MAO-A. (ⅴ) The compound 2 showed weak inhibiting ability against both MAO-A and MAO-B. These findings suggest that the compound 1 having strong inhibiting ability against MAO-B could lead to the development of functional food materials intended to improve brain function.

著者

久保田 浩樹 佐藤 恭子 佐々木 伸夫 河村 葉子 小関 良宏 穐山 浩

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.19, no.2, pp.94-103, 2012

参考文献数

38

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カットキャベツを次亜塩素酸ナトリウムにより殺菌処理したときに生成する消毒副生成物の生成影響因子について検討を行った。カットキャベツは、次亜塩素酸ナトリウム(100mg/L)単独あるいは、有機酸と共に10分間殺菌処理を行い、カットキャベツに残存する揮発性ハロゲン化合物をヘッドスペースガスクロマトグラフ質量分析装置で測定した。主要な副生成物としてクロロホルムが検出された。クロロホルムは、殺菌時間、pH、温度、初期次亜塩素酸ナトリウム濃度に依存して増加した。次亜塩素酸溶液を種々の無機酸及び有機酸と併用してもトリハロメタンは生成しないが、クエン酸は次亜塩素酸と反応し、クロロホルムを生成した。クロロホルム濃度は、次亜塩素酸・クエン酸混液の混和時間に対応して増加し、カット野菜の殺菌時間には影響しなかった。また、カット野菜に残存したクロロホルムは水洗浄により、水道水中のトリハロメタン濃度レベルまで減少した。

著者

岩﨑 啓子 出口 雄也 長岡(浜野) 恵 長岡 寛明 野村 秀一

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.17, no.1, pp.65-68, 2010-04-14 (Released:2017-01-27)

参考文献数

8

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A high performance liquid chromatographic method for the simultaneous determination of malic acid, acetic acid, citric acid, amygdalin, benzyl alcohol, benzaldehyde, and benzoic acid in pickled Japanese apricots (Umeboshi) was developed. TSKgel ODS-100s was used as the separation column, and the mixture of 0.1% phosphoric acid and methanol was used as mobile phase. Detection wavelength was at 210nm. All compounds were eluted within 30 minutes. In three commercial pickled Japanese apricots, 4μg/g -46mg/g of malic acid, acetic acid, citric acid and benzyl alcohol, benzaldehyde were detected. In the pickled Japanese apricots, amygdalin was detected.

著者

奥村 寿子 一谷 正己 瀧原 孝宣 国本 浩喜

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.14, no.3, pp.128-133, 2008-01-10 (Released:2017-12-01)

参考文献数

18

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UV absorption spectra of authentic tea catechins were measured in the pH range between 5.0-10.0. Gallated and non-gallated catechins exhibited quite different pH dependence to each other. On raising the pH from 5.0 to 8.7, the UV absorption maxima of gallated catechins typically shift from 274 nm to 322 nm with a distinct isosbestic point around 290 nm. On further raising the pH to 10, the absorption intensity at 322 nm became more intense with the spectra deviating from the isosbestic point. On the other hand, non-gallated catechins showed small spectral changes upon these pH changes. The content of gallated catechins contained in commercial tea beverages and tea extracts are determined on the basis of the UV absorption difference between gallated and non-gallated catechins. Results of the UV absorption method showed good correspondence with those by the HPLC method.