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1 0 0 0 OA Analysis of IgG4 class switch-related molecules in IgG4-related disease

著者: Tsuboi Hiroto Matsuo Naomi Iizuka Mana Tsuzuki Sayaka Kondo Yuya Tanaka Akihiko Moriyama Masafumi Matsumoto Isao Nakamura Seiji Sumida Takayuki
出版者: BioMed Central
雑誌: Arthritis research & therapy (ISSN:14786354)
巻号頁・発行日: vol.14, no.4, pp.R171, 2012-07
被引用文献数: 84 19

IntroductionImmunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a new disease entity characterized by high serum IgG4 levels, IgG4-positive plasmacytic infiltration, and fibrosis in various organs. The purpose of this study was to determine the mechanism of upregulation of IgG4 class switch recombination in IgG4-RD.MethodsWe extracted RNA from peripheral blood mononuclear cells (PBMCs) of patients with IgG4-RD (n = 6), Sjögren syndrome (SS) (n = 6), and healthy controls (n = 8), from CD3-positive T cells and CD20-positive B cells sorted from PBMCs of patients with IgG4-RD (n = 3), SS (n = 4), and healthy controls (n = 4), as well as from labial salivary glands (LSGs) of patients with IgG4-RD (n = 11), SS (n = 13), and healthy controls (n = 3). The mRNA expression levels of IgG4-specific class switch-related molecules, such as Th2 cytokines (IL-4 and IL-13), Treg cytokines (IL-10 and TGF-β), and transcriptional factors (GATA3 and Foxp3) were examined with quantitative polymerase chain reaction (PCR). IgG4-nonspecific class switch-related molecules, such as CD40, CD154, BAFF, APRIL, IRF4, and AID, were also examined.ResultsThe expression levels of Treg cytokines (IL-10 and TGF-β) and AID were significantly higher in LSGs of IgG4-RD than in SS and the controls (P < 0.05, each). In contrast, those of CD40 and CD154 were significantly lower in PBMCs of IgG4-RD than in SS (P < 0.05, each), whereas CD40 in CD20-positive B cells and CD154 in CD3-positive T cells were comparable in the three groups.ConclusionOverexpression of IL-10, TGF-β, and AID in LSGs might play important roles in the pathogenesis of IgG4-RD, such as IgG4-specific class-switch recombination and fibrosis. IgG4 class-switch recombination seems to be mainly upregulated in affected organs.

2013-03-23 08:30:28
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1 0 0 0 OA 骨格筋拡散テンソル画像の臨床応用の試み

著者: 岡本嘉一
巻号頁・発行日: 2012

科学研究費助成事業(科学研究費補助金)研究成果報告書:若手研究(B)2009-2011

2013-03-23 08:30:23
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http://hdl.handle.net/2241/118716

1 0 0 0 OA Studies of millimeter-wave imaging array for the GAMMA 10 tandem mirror

著者: Oyama Naoyuki
巻号頁・発行日: 1999

Thesis (Ph. D. in Science)--University of Tsukuba, (A), no. 2064, 1999.3.25

2013-03-23 08:30:12
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http://hdl.handle.net/2241/5465

1 0 0 0 OA IgG4関連硬化性胆管炎の診断と治療

著者: 中沢貴宏大原弘隆城卓志
出版者: 日本胆道学会
雑誌: 胆道 (ISSN:09140077)
巻号頁・発行日: vol.24, no.4, pp.569-578, 2010 (Released:2011-01-07)
参考文献数: 30
被引用文献数: 4

要旨:IgG4関連硬化性胆管炎は原発性硬化性胆管炎や胆管癌との鑑別が必要である.自己免疫性膵炎の合併の有無,血清IgG4値,全身合併症を参考に,胆管像,管腔内超音波像で診断することが重要である.胆管の生検診断は胆管癌の除外に有用である.膵病変が明らかでなく,硬化性胆管炎のみを認める症例は診断に難渋する.治療は自己免疫性膵炎と同様にステロイドの投与で軽快するが,再燃を繰り返す症例やステロイドに反応が悪い症例に対して海外では免疫抑制剤の併用も行われている.予後はおおむね良好であるが,肝萎縮をきたす症例も報告されている.