@ChenxinLi2 There was a trial of Wasabi tissue culture in 1988. Pungent sinigrin was produced in differentiated tissue but not in callus. MYB28 overexpression may be needed?
https://t.co/XCHzvKujY1
@0_minyaaa This 1987 paper reported that the white and purple morphs of Sisyrinchium can be crossed and that white is dominant! So I wished to reproduce it as my hobby experiment. But.. (1/n)
https://t.co/YnujPRxm70
Curious to see the spherical drops and unwetted flower buds. Is it OK to omit any surfactant like Silwet L-77?
I follow a related protocol from Narusaka et al. (2010):
https://t.co/YUcYlqZent https://t.co/73VOdOk7Pn
The floral inoculating protocol, reported by Narusaka et al. (2010), is even more simple that it only needs 5 μL Agrobacterium suspension per inflorescence.
I personally call it "floral dripping"