著者

ISIS-4

雑誌

Lancet

巻号頁・発行日

vol.345, pp.669-685, 1995

被引用文献数

37 1452 54

著者

editor-in-chief Tatau Nishinga volume editor Peter Rudolph

出版者

Elsevier

巻号頁・発行日

2015

著者

OKUBO Yoshiko MIQUEL Jean-Francois

出版者

研究・イノベーション学会

雑誌

研究技術計画 (ISSN:09147020)

巻号頁・発行日

vol.6, no.4, pp.261-280, 1992-10-15

被引用文献数

2

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1981年から86年までの科学引用索引データを基に2国間の共著論文数を指標化し,日本と世界97カ国との共同研究を定量的に測定した。また,日本の共同研究状況をマクロとミクロの両方から分析した。日本の基礎研究における国際化の水準は低いが進んでいた。共著の相手国は米国の比重が大きいもののアジア諸国及び西ヨーロッパ諸国との研究が増加していた。共同研究は分野により国際化の状況が異なり,相手国によっても共同研究様式が違っていた。8科学分野における日本と10数カ国間の関係構造を検討し,各国々の日本における位置および日本の各国における位置を示した。具体例として日本とスウェーデン,イタリア,スイス,フランス,中国との科学技術活動を検討し,さらに神戸大学とフランスとの共同研究を取り上げて国際ネットワークの事例を分析した。

著者

edited by Lars-Göran Nilsson

出版者

Distributed by Halsted Press Division of Wiley

巻号頁・発行日

1979

著者

re-told by Marie Crook

出版者

Pearson Education

巻号頁・発行日

2012

著者

KIKE-KWONG CHEN

出版者

Mathematical Institute, Tohoku University

雑誌

Tohoku Mathematical Journal, First Series (ISSN:00408735)

巻号頁・発行日

vol.20, pp.44-47, 1921 (Released:2010-03-19)

著者

Young-Hoon Song Soo-Jin Jeong Hee-Young Kwon Bonglee Kim Sung-Hoon Kim Dong-Youl Yoo

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.35, no.7, pp.1022-1028, 2012-07-01 (Released:2012-07-01)

参考文献数

33

被引用文献数

13 39

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Although ursolic acid isolated from Oldenlandia diffusa (Rubiaceae) was known to have anticancer activities in prostate, breast and liver cancers, the underlying mechanism of ursolic acid in ovarian cancer cells was not investigated so far. In the present study, the apoptotic mechanism of ursolic acid was elucidated in SK-OV-3 ovarian cancer cells by 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, cell cycle analysis and Western blotting. Ursolic acid exerted cytotoxicity against SK-OV-3 and A2780 ovarian cancer cells with IC50 of ca. 50 and 65 µM, respectively. Apoptotic bodies were observed in ursolic acid treated SK-OV-3 cells. Also, ursolic acid significantly increased ethidium homodimer stained cells and sub-G1 apoptotic portion in SK-OV-3 cells. Consistently, Western blotting revealed that ursolic acid effectively cleaved poly(ADP-ribose) polymerase (PARP), caspase-9 and -3, suppressed the expression of survival genes such as c-Myc, Bcl-xL and astrocyte elevated gene (AEG)-1, and upregulated phosphorylation of extracellular signal-regulated kinase (ERK) in SK-OV-3 cells. Interestingly, ursolic acid suppressed β-catenin degradation as well as enhanced phosphorylation of glycogen synthase kinase 3 beta (GSK 3β). Furthermore, GSK 3β inhibitor SB216763 blocked the cleavages of caspase-3 and PARP induced by ursolic acid and proteosomal inhibitor MG132 disturbed down-regulation of β-catenin, activation of caspase-3 and decreased mitochondrial membrane potential (MMP) induced by ursolic acid in SK-OV-3 cells. Overall, our findings suggest that ursolic acid induces apoptosis via activation of caspase and phosphorylation of GSK 3β in SK-OV-3 cancer cells as a potent anti-cancer agent for ovarian cancer therapy.

著者

Kyung-Ah Jung Tae-Chul Song Daeseok Han In-Ho Kim Young-Eon Kim Chang-Ho Lee

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.28, no.9, pp.1782-1785, 2005 (Released:2005-09-01)

参考文献数

27

被引用文献数

43 88

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It is currently accepted that the consumption of fruit-derived antioxidants such as vitamin C, carotenoids, and flavonoids provides a preventive effect against cardiovascular disease. The purpose of the present study was to investigate potential cardiovascular protective properties of aqueous and 70% ethanol extracts from kiwifruit by analyzing the antioxidative, antihypertensive, hypocholesterolemic, and fibrinolytic activities in vitro. Aqueous and 70% ethanol extracts at 50 mg/ml showed DPPH-radical scavenging activities of 72.31% and 70.75%, respectively. Total antioxidant activity in linoleic acid emulsion was 85—88% at 10 mg/ml and 96—98% at 50 mg/ml of kiwifruit extract. Inhibitory activities against angiogensin I-converting enzyme of kiwifruit extracts were 21—26% at 10 mg/ml and 46—49% at 50 mg/ml, and inhibitory activities on HMG-CoA reductase were 13—14% at 10 mg/ml and 19—30% at 50 mg/ml. Fibrinolytic activity of kiwifruit was also observed at a high concentration of 100 mg/ml in both aqueous and 70% EtOH extracts. Based on our results, kiwifruit have potential cardiovascular protective properties in vitro.

著者

山下 英俊 戸張 幾生 澤 充 堀 貞夫 宮園 浩平 HELDIN C-H HELDIN P DIJKE P ten SAMPATH T Kuber 水流 忠彦 江口 秀一郎 北野 滋彦 鈴木 水音 一條 秀憲 加藤 光保 山本 禎子 船津 英陽 鈴木 雅信 池上 靖子 加藤 聡 小幡 博人 堀江 公仁子 茂木 豊 征矢 耕一 田中 義和 清水 顕 加治 優一 出田 隆一 三田 知直 山田 秀之 石田 耕一 東 マルシア 羽生 亜紀 三浦 雅一 白澤 榮一 酒井 宏之

雑誌

日本眼科學会雜誌 (ISSN:00290203)

巻号頁・発行日

vol.101, no.12, pp.927-947, 1997-12-10

被引用文献数

6

著者

Ikeda Masahiko Komatsu Shin-ya Nakamura Yuichiro

出版者

公益社団法人 日本金属学会

雑誌

MATERIALS TRANSACTIONS

巻号頁・発行日

vol.45, no.4, pp.1106-1112, 2004

被引用文献数

43

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Using Ti-50Ta, Ti-50Ta-Sn and Ti-50Ta-Zr alloys, the effects of Sn and Zr additions on phase constitution in the solution treated and quenched state and aging behavior were studied by electrical resistivity and Vickers hardness measurements and X-ray diffactometry. All alloys were solution treated at 1173 K for 3.6 ks and then quenched into ice water (STQ). STQed specimens were isochronally aged at temperatures up to 1323 K in Ti-50Ta-Sn alloys and 1173 K in Ti-50Ta-Zr alloys. Shape recovery test was also performed in bent Ti-50Ta, Ti-50Ta-Sn and Ti-50Ta-Zr alloy specimens. In the STQed state, only reflections of orthorhombic α″ martensite were observed by XRD in Ti-50Ta and Ti-50Ta-4Sn alloys. In STQed Ti-50Ta-10Zr alloy, coexistence of α″ and β (bcc) phases were found by XRD. In STQed Ti-50Ta-8Sn and Ti-50Ta-20Zr alloys, only β phase was identified. In Ti-50Ta, Ti-50Ta-4Sn and Ti-50Ta-10Zr alloys, resistivity at liquid nitrogen temperature and resistivity ratio increased with isochronal aging up to a certain temperature. It is considered that these increases are due to reverse-transformation of α″ into metastable β phase. Shape-recovery test confirmed the shape memory effect of Ti-50Ta, 50Ta-4Sn and 50Ta-10Zr alloys.

著者

מאת אהרון פולד מפרנקפורט = Sefer Hagahot he-Aruch / by Aaron Fuld of Frankfurt . מאת אברהם ב''ר שמואל מיראנדה = Kuntres Morehbe-Eẓba / by Abraham b.R. Samuel of Jaronda

出版者

מקור

巻号頁・発行日

1978

著者

Freitag-Leiptig R.

出版者

日本医療機器学会

雑誌

醫科器械學雜誌 (ISSN:00191736)

巻号頁・発行日

vol.13, no.6, pp.283-289, 1935-12-20

著者

Jury KIM Jiseong HEO Dongbeom JI Min-Su KIM

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0387, (Released:2014-12-25)

被引用文献数

1 16

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The purpose of this study was to quantitatively assess the pupillary light reflex (PLR) in normal and anesthetized dogs using a pupillometer. Eleven dogs (20 eyes) of various breeds were included. PLRs were measured with a handheld pupillometer in dim light before and during anesthesia. Anesthesia was conducted with atropine, xylazine and ketamine. Parameters of pupillometry included neurological pupil index (NPi), pupil size, percent of change (%CH), latency (LAT), constriction velocity (CV), maximum constriction velocity (MCV) and dilation velocity (DV). NPi, %CH, CV and MCV were significantly decreased during anesthesia compared with the pre-anesthesia data. The results suggest that atropine-xylazine-ketamine combination anesthesia depresses the PLR. Additionally, this study demonstrates the feasibility of the use of a pupillometer in dogs.

著者

Hiroko P. Indo Hsiu-Chuan Yen Ikuo Nakanishi Ken-ichiro Matsumoto Masato Tamura Yumiko Nagano Hirofumi Matsui Oleg Gusev Richard Cornette Takashi Okuda Yukiko Minamiyama Hiroshi Ichikawa Shigeaki Suenaga Misato Oki Tsuyoshi Sato Toshihiko Ozawa Daret K. St. Clair Hideyuki J. Majima

出版者

日本酸化ストレス学会

雑誌

Journal of Clinical Biochemistry and Nutrition (ISSN:09120009)

巻号頁・発行日

pp.14-42, (Released:2014-12-23)

参考文献数

90

被引用文献数

36 232

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Fridovich identified CuZnSOD in 1969 and manganese superoxide dismutase (MnSOD) in 1973, and proposed ”the Superoxide Theory,” which postulates that superoxide (O2•−) is the origin of most reactive oxygen species (ROS) and that it undergoes a chain reaction in a cell, playing a central role in the ROS producing system. Increased oxidative stress on an organism causes damage to cells, the smallest constituent unit of an organism, which can lead to the onset of a variety of chronic diseases, such as Alzheimer’s, Parkinson’s, amyotrophic lateral sclerosis and other neurological diseases caused by abnormalities in biological defenses or increased intracellular reactive oxygen levels. Oxidative stress also plays a role in aging. Antioxidant systems, including non-enzyme low-molecular-weight antioxidants (such as, vitamins A, C and E, polyphenols, glutathione, and coenzyme Q10) and antioxidant enzymes, fight against oxidants in cells. Superoxide is considered to be a major factor in oxidant toxicity, and mitochondrial MnSOD enzymes constitute an essential defense against superoxide. Mitochondria are the major source of superoxide. The reaction of superoxide generated from mitochondria with nitric oxide is faster than SOD catalyzed reaction, and produces peroxynitrite. Thus, based on research conducted after Fridovich’s seminal studies, we now propose a modified superoxide theory; i.e., superoxide is the origin of reactive oxygen and nitrogen species (RONS) and, as such, causes various redox related diseases and aging.

著者

Seong-Gyu Ko Seung-Hee Koh Chan-Yong Jun Chang-Gyu Nam Hyun-Su Bae Min-Kyu Shin

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.27, no.10, pp.1604-1610, 2004 (Released:2004-10-01)

参考文献数

28

被引用文献数

25 56

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We performed this study to understand the molecular basis underlying the antitumor effects of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatments of Saussurea lappa and Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica and Taraxacum mongolicum didn't. FACS analysis and Annexin V staining assay also showed that both Saussurea lappa and Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa, but not Pharbitis nil, increased expression of the p53 and its downstream effector p21Waf1, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa and Pharbitis nil induce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.

著者

Kocku von Stuckrad translated and with a foreword by Nicholas Goodrick-Clarke

出版者

Equinox Pub.

巻号頁・発行日

2005

著者

Hirotaka IGARASHI Koichi OHNO Aki FUJIWARA-IGARASHI Hideyuki KANEMOTO Kenjiro FUKUSHIMA Yuko GOTO-KOSHINO Kazuyuki UCHIDA Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0505, (Released:2014-12-20)

被引用文献数

3 12

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Inflammatory colorectal polyps (ICRPs) frequently occur in miniature dachshunds (MDs) in Japan. MDs with ICRPs develop multiple polyps with severe neutrophil infiltration that respond to immunosuppressive therapy. Therefore, ICRPs are thought to constitute a novel, breed-specific form of canine inflammatory bowel disease (IBD). Pattern recognition receptors (PRRs) play a key role in the distinction of pathogens from commensal bacteria and food antigens. Dysfunction resulting from genetic disorders of PRRs have been linked to human and canine IBD. Therefore, we analyzed the reactivity of PRRs in MDs with ICRPs. Twenty-six MDs with ICRPs and 16 control MDs were recruited. Peripheral blood-derived monocytes were obtained from each dog and then stimulated with PRR ligands for 6 and 24 hr; subsequently, messenger RNA (mRNA) expression levels and protein secretion of IL-1β were quantified using quantitative real-time PCR and ELISA, respectively. The levels of IL-1β mRNA and protein secretion after stimulation with a nucleotide-binding oligomerization domain 2 (NOD2) ligand were significantly greater in monocytes from ICRP-affected MDs than in those from control MDs. In addition, IL-1β protein secretion induced by toll-like receptor (TLR) 1/2, TLR2 and TLR2/6 stimulation was also significantly greater in ICRP-affected MDs. These results suggest that reactivity against NOD2, TLR1/2, TLR2 and TLR2/6 signals is enhanced in ICRP-affected MDs and may play a role in the pathogenesis of ICRPs in MDs. Additional studies of the genetic background of these PRRs should be performed.

著者

Yoshiki Murakami Toshihito Tanahashi Rika Okada Hidenori Toyoda Takashi Kumada Masaru Enomoto Akihiro Tamori Norifumi Kawada Y-H. Taguchi Takeshi Azuma

出版者

一般社団法人情報処理学会

雑誌

研究報告バイオ情報学(BIO) (ISSN:09196072)

巻号頁・発行日

vol.2014, no.2, pp.1-5, 2014-12-11

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Although microarray has been an important tool that can perform extensive gene expression analyses, next generation sequencing (NGS) has recently arisen as an alternative methodology that can measure gene expression. In this paper, we have compared microarray and NGS quantitatively using microRNA measurements in hepatocellular carcinoma (HCC) and found that these two are coincident with each other. NGS also turned out to be used for biomarker between HCC and normal tissue if the recently proposed principal component analysis based unsupervised feature extraction was applied.Although microarray has been an important tool that can perform extensive gene expression analyses, next generation sequencing (NGS) has recently arisen as an alternative methodology that can measure gene expression. In this paper, we have compared microarray and NGS quantitatively using microRNA measurements in hepatocellular carcinoma (HCC) and found that these two are coincident with each other. NGS also turned out to be used for biomarker between HCC and normal tissue if the recently proposed principal component analysis based unsupervised feature extraction was applied.

著者

Nojima Hisatake Katamine Daisuke Kawashima Kenjiro Nakajima Yasuo Imai Jun-ichi Sakamoto Makoto Shimada Masaaki Miyahara Michiaki

出版者

日本熱帯医学会

雑誌

日本熱帯医学会雑誌 (ISSN:03042146)

巻号頁・発行日

vol.6, no.3, pp.181-193, 1978-12-15

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ケニア国タベタ地区での淡水産貝類は以下の8属11種である。即ちBiomphalaria pfeifferi(Krauss),B. sudanica (Martens),Bulinus globosus (Morelet),B. tropicus (Krauss),B.forskalii (Ehrenberg),以上5種は住血吸虫との関係種,Lymnea natalensis (Krauss),Ceratophallus natalensis(Krauss),Segmentorbis angustus (Jickeli),Gyraulus costulatus (Krauss),Bellamya unicolor (Olivier),Melanoides tuberculata (Muller)である。B. pfeifferiはLumi川と灌漑用溝に, B. sudanicaはJipe湖畔に,それぞれの多数の棲息をみたが,マンソン住血吸虫の自然感染はB. pfeifferiのみに見られた。B.globosusは灌漑用溝のみに多数棲息し,B. tropicusは灌漑用溝とJipe湖畔に,B. forskaliiは少数ながらあらゆる水系に見出された。ビルハルツ住血吸虫の自然感染はB. globosusのみに見出され,その貝の棲息数が多いと約10%の高い感染率が常時認められた。一方これらの実験感染では,B. pfeifferiには3隻のミラシジウムで,B. sudanicaには5隻のそれで100%感染が成立し,両種ともマンソン住血吸虫の好適な中間宿主であることがわかった。B. globosusは1.5~8.5mmの若い貝は5隻のミラシジウムで100%感染が成立し,11~12mmの成貝では20隻以上のミラシジウムが必要である。ビルハルツ住血吸虫の好適な中間宿主であることがわかった。以上からタベタ地区でのマンソン住血吸虫症,ピノレハルツ住血吸虫症の媒介中間宿主として,前者にはB. pfeifferiとB. sudanicaが,後者にはB.globosusが主な役割を演じていることが推測される。The present study was carried out in the permanent water streams of Lumi River, Irrigation Furrow and Lake Jipe in the Taveta area, Coast Province, Kenya during the dry seasons of 1974 and 1975, and the experimental infection was made at laboratory in Japan. Freshwater snails collected in the Taveta area were as follows: Biomphalaria pfeifferi (Krauss), B. sudanica (Martens), Bulinus globosus (Morelet), B. tropicus (Krauss), B. forskalii (Ehrenberg), Lymnea natalensis (Krauss), Ceratophallus natalensis (Krauss), Segmentorbis angustus (Jickeli), Gyraulus costulatus (Krauss), Bellamya unicolor (Olivier) and Melanoides tuberculata (Muller). B. pfeifferi was commonly found in river and irrigation canal, whereas B. sudanica only in lake. Natural infection of Schistosoma mansoni was found in B. pfeifferi, but not in B. sudanica. Both the two species were experimentally proved to be suitable intermediate snail hosts of S. mansoni. Therefore it was indicated that B. pfeifferi is the host snail of S. mansoni in the endemic area along river and irrigation canal while B. sudanica is suspected of playing the role in the transmission of S. mansoni in lakeshore. B. globosus was commonly found in irrigation canal. Around 10 per cent of the snails proved to be naturally infected with S. haematobium on the conditions that many snails occurred. This snail was also experimentally proved to be susceptible to S. haematobium. B. forskalii was widespread, but the snail density seemed to be low. B. tropicus is well known as the not-intermediate snail host of S. haematobium. Therefore there might be a possibility to contribute only by B. globosus to the transmission of S. haematobium in this area.

著者

Nakagawa Takayuki Kumakawa Kozo Usami Shin-ichi Hato Naohito Tabuchi Keiji Takahashi Mariko Fujiwara Keizo Sasaki Akira Komune Shizuo Sakamoto Tatsunori Hiraumi Harukazu Yamamoto Norio Tanaka Shiro Tada Harue Yamamoto Michio Yonezawa Atsushi Ito-Ihara Toshiko Ikeda Takafumi Shimizu Akira Tabata Yasuhiko Ito Juichi

出版者

BioMed Central Ltd.

雑誌

BMC medicine (ISSN:17417015)

巻号頁・発行日

vol.12, 2014-11-19

被引用文献数

74

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突発性難聴に対する新規治療法の有効性を報告. 京都大学プレスリリース. 2014-12-10.