著者

辻 威彦 籠谷 和弘 梅本 善明 松尾 雄志

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.27, no.2, pp.60-66, 2020 (Released:2020-08-31)

参考文献数

18

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Ginger (Zingiber officinale) rhizomes have been reported to exhibit multiple efficacies on human health. In this paper, we found that n-hexane extract from ginger rhizomes (ginger oil) exhibited the inhibiting abilities against monoamine oxidase A (MAO-A) and B (MAO-B), which catalyze the oxidative deamination of monoamine neurotransmitters, by using an in vitro luminescencebased method. To characterize the compounds responsible for the inhibiting abilities against MAO-A and MAO-B, all fractions from reversed-phase chromatographies were subjected to the luminescence assay. As a result, we isolated five compounds from ginger oil by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The half-maximal inhibitory concentration (IC50) measurement using purified compounds revealed that (ⅰ) 6-, 8-, and 10-gingerols had strong inhibiting ability against MAO-A, whereas they showed weak inhibiting ability against MAO-B. (ⅱ) IC50 values of 6-, 8-, and 10-gingerols against MAO-A were calculated to be 175.7±11.0 μM, 54.6±2.0 μM, and 113.4±4.7 μM, respectively. (ⅲ) Furthermore, we revealed that 1-dehydro- [6]-gingerdione (compound 1) and diacetoxy-[6]-gingerdiol (compound 2) were contained in the ginger oil. (ⅳ) The compound 1 showed strong and selective inhibiting ability against MAO-B with IC50 of 6.77±0.45 μM, which was 10 times stronger than that against MAO-A. (ⅴ) The compound 2 showed weak inhibiting ability against both MAO-A and MAO-B. These findings suggest that the compound 1 having strong inhibiting ability against MAO-B could lead to the development of functional food materials intended to improve brain function.

著者

久保田 浩樹 佐藤 恭子 佐々木 伸夫 河村 葉子 小関 良宏 穐山 浩

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.19, no.2, pp.94-103, 2012

参考文献数

38

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カットキャベツを次亜塩素酸ナトリウムにより殺菌処理したときに生成する消毒副生成物の生成影響因子について検討を行った。カットキャベツは、次亜塩素酸ナトリウム(100mg/L)単独あるいは、有機酸と共に10分間殺菌処理を行い、カットキャベツに残存する揮発性ハロゲン化合物をヘッドスペースガスクロマトグラフ質量分析装置で測定した。主要な副生成物としてクロロホルムが検出された。クロロホルムは、殺菌時間、pH、温度、初期次亜塩素酸ナトリウム濃度に依存して増加した。次亜塩素酸溶液を種々の無機酸及び有機酸と併用してもトリハロメタンは生成しないが、クエン酸は次亜塩素酸と反応し、クロロホルムを生成した。クロロホルム濃度は、次亜塩素酸・クエン酸混液の混和時間に対応して増加し、カット野菜の殺菌時間には影響しなかった。また、カット野菜に残存したクロロホルムは水洗浄により、水道水中のトリハロメタン濃度レベルまで減少した。

著者

岩﨑 啓子 出口 雄也 長岡(浜野) 恵 長岡 寛明 野村 秀一

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.17, no.1, pp.65-68, 2010-04-14 (Released:2017-01-27)

参考文献数

8

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A high performance liquid chromatographic method for the simultaneous determination of malic acid, acetic acid, citric acid, amygdalin, benzyl alcohol, benzaldehyde, and benzoic acid in pickled Japanese apricots (Umeboshi) was developed. TSKgel ODS-100s was used as the separation column, and the mixture of 0.1% phosphoric acid and methanol was used as mobile phase. Detection wavelength was at 210nm. All compounds were eluted within 30 minutes. In three commercial pickled Japanese apricots, 4μg/g -46mg/g of malic acid, acetic acid, citric acid and benzyl alcohol, benzaldehyde were detected. In the pickled Japanese apricots, amygdalin was detected.

著者

奥村 寿子 一谷 正己 瀧原 孝宣 国本 浩喜

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.14, no.3, pp.128-133, 2008-01-10 (Released:2017-12-01)

参考文献数

18

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UV absorption spectra of authentic tea catechins were measured in the pH range between 5.0-10.0. Gallated and non-gallated catechins exhibited quite different pH dependence to each other. On raising the pH from 5.0 to 8.7, the UV absorption maxima of gallated catechins typically shift from 274 nm to 322 nm with a distinct isosbestic point around 290 nm. On further raising the pH to 10, the absorption intensity at 322 nm became more intense with the spectra deviating from the isosbestic point. On the other hand, non-gallated catechins showed small spectral changes upon these pH changes. The content of gallated catechins contained in commercial tea beverages and tea extracts are determined on the basis of the UV absorption difference between gallated and non-gallated catechins. Results of the UV absorption method showed good correspondence with those by the HPLC method.

著者

山田 恭正 大見 のり子 山根 美保 中谷 延二

出版者

Japanese Society of Food Chemistry

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.19, no.1, pp.59-63, 2012-04-23 (Released:2017-01-27)

参考文献数

16

被引用文献数

1

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沖縄県で栽培されている主な有色甘藷は「備瀬」(ビセ)であるが、育種により「沖夢紫」(オキユメムラサキ)が作出され2007年に品種登録された。備瀬と沖夢紫に含まれるアントシアニンをHPLCで比較し、沖夢紫に特徴的な2種のアントシアニンを単離精製した。LCMS、NMR等で化学構造を解析した結果、非アシル化アントシアニンのcyanidin 3-sophoroside-5-glucoside、モノアシル化アントシアニンのpeonidin 3-(6-caffeoyl-sophoroside)-5-glucosideを同定した。非アシル化、モノアシル化アントシアニンは有色甘藷を摂取後、小腸で直接吸収されるので、これらのアントシアニンが沖夢紫から単離、同定されたことは生理学的な活性を評価する上で興味深い。

著者

梅 琳 小原 章裕 松久 次雄

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.13, no.3, pp.141-145, 2006-12-30 (Released:2017-12-01)

参考文献数

18

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Effects of linoleic acid, α-linolenic acid and soybean oil administration on the autoimmune factors of MRL/lpr strain mice. Male mice of 5 weeks of age were divided into three groups; Group 1(Soybean oil group), Group 2(Linoleic acid group), Group 3 (α-linolenic acid group). Mice were individually housed in steel cages and the diets and water were fed ad libitum. Weight of alae lymph node of mice fed α-linolenic acid diet was significantly lower than that of mice fed linoleic acid diet. Protein urea concentration of the mice fed α-linolenic acid diet was lower than that of the linoleic acid diet group. Serum anti-dsDNA antibody titre , IL-18 and TNF-α of mice were decreased by administering α-linolenic acid. Mice fed α-linolenic acid showed highest survival rate. Soybean oil diet gave the intermediate value between α-linolenic acid and linoleic acid. These results well agreed with the previous reports of cooking oils such as perilla oil, fish oil etc.

著者

髙橋 美津子 桜井 克巳 菅谷 なえ子 刈込 髙子 斉藤 貢一

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.24, no.1, pp.1-9, 2017 (Released:2017-04-29)

参考文献数

18

被引用文献数

1

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To verify the presence of yohimbine and its four stereoisomers (corynanthine, α-yohimbine, β-yohimbine, and isorauhimbine) in health food and to perform a quantitative analysis, we examined the separation and detection conditions for each component by GC-MS and LC-UV-MS. As a result, in GC-MS analysis, baseline separation of all five components (yohimbine and its four stereoisomers) was achieved with a mid-polar column. However, the presence of a water desorption peak due to heating, calibration curves with poor linearity, and low recovery rates suggested that the use of GC-MS for quantitative analysis required further consideration. On the other hand, in LC-UV-MS analysis, baseline separation of all the five components was possible when a mixture of ammonium bicarbonate buffer and acetonitrile was used as the mobile phase. In addition, calibration curves showed adequate linearity with good recovery rates. On the basis of these results, we decided to employ the analytical condition by LC-UVMS. Using this method, we analyzed commercial health food to determine the actual contents of yohimbine and its stereoisomers, and identified food products in which only yohimbine was detected, as well as those in which yohimbine and its four stereoisomers were detected. Furthermore, we found at a high frequency food products in which corynanthine and α-yohimbine were detected at higher concentrations than yohimbine.

著者

Agus Bahar Rachman 赤澤 隆志 小川 雅廣

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.27, no.1, pp.10-21, 2020

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Olive leaf (<i>Olea europaea</i> L.) is an underutilized resource of olive tree but has a high polyphenol content, so it has the potential to be used as a food ingredient. In this study, we investigated the effects of olive leaf water extracts on the physical properties of chicken breast sausage (CBS). Olive leaf water extracts were prepared at 4 (OEx₄) and 80°C (OEx₈₀) from dried olive leaf powder and they were applied to CBS preparation within the concentration range 0.05 to 0.5% (w/w minced meat). OEx₄ had 32% lower polyphenol content than OEx₈₀. Water content, expressible water, breaking strength, and viscoelastic properties of CBS with OEx₄ and OEx₈₀ were measured. The analyses of the sausage physical properties showed that adding 0.1% OEx₄ increased the breaking stress (115%), breaking strain (68%), modulus of elasticity (20%), coefficient of viscosity (23%), and water-holding capacity (9%), and the enhancement effects of OEx₄ were much greater than those of OEx₈₀. The gel network of OEx₄-containing CBSs observed by scanning electron microscopy was finer and denser than that of the control CBS. Chemical analyses of the meat proteins showed that OEx₄ induces polymerization of myofibril proteins, especially myosin, via non-disulfide covalent bridges formed between amino and/ or thiol groups. These results indicate that the application of OEx₄ improves the sausage gel properties by inducing non-disulfide-type protein polymerization. This study supports the feasibility of OEx₄ as a new ingredient to improve the texture of gel products prepared from chicken breast minced meat.

著者

Agus Bahar Rachman 赤澤 隆志 小川 雅廣

出版者

Japanese Society of Food Chemistry

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.27, no.1, pp.10-21, 2020 (Released:2020-04-30)

参考文献数

35

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Olive leaf (Olea europaea L.) is an underutilized resource of olive tree but has a high polyphenol content, so it has the potential to be used as a food ingredient. In this study, we investigated the effects of olive leaf water extracts on the physical properties of chicken breast sausage (CBS). Olive leaf water extracts were prepared at 4 (OEx4) and 80°C (OEx80) from dried olive leaf powder and they were applied to CBS preparation within the concentration range 0.05 to 0.5% (w/w minced meat). OEx4 had 32% lower polyphenol content than OEx80. Water content, expressible water, breaking strength, and viscoelastic properties of CBS with OEx4 and OEx80 were measured. The analyses of the sausage physical properties showed that adding 0.1% OEx4 increased the breaking stress (115%), breaking strain (68%), modulus of elasticity (20%), coefficient of viscosity (23%), and water-holding capacity (9%), and the enhancement effects of OEx4 were much greater than those of OEx80. The gel network of OEx4-containing CBSs observed by scanning electron microscopy was finer and denser than that of the control CBS. Chemical analyses of the meat proteins showed that OEx4 induces polymerization of myofibril proteins, especially myosin, via non-disulfide covalent bridges formed between amino and/ or thiol groups. These results indicate that the application of OEx4 improves the sausage gel properties by inducing non-disulfide-type protein polymerization. This study supports the feasibility of OEx4 as a new ingredient to improve the texture of gel products prepared from chicken breast minced meat.

著者

北元 憲利 田中 智之 加藤 陽二 辻 啓介

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.7, no.2, pp.86-92, 2000-10-28 (Released:2017-12-01)

参考文献数

13

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こんにゃくは、水酸化カルシウム溶液に浸されたアルカリ性を呈する食品であるが、腸管出血性大腸菌O157との関わりを検討した報告はない。そこで、本研究では、こんにゃく液中におけるO157の生存状況と、こんにゃく液の抗菌効果(静菌作用あるいは殺菌作用)について検討した。こんにゃく液はいずれもpH12前後であり、この高いpH域では、O157は反応させるだけで検出限界までに激減することがわかった。初発菌数が多い場合でも1日の反応(保存)でほとんどが死滅した。また、この抗菌作用は大腸菌の株による違いや温度差には影響されなかった。一方、食中毒菌であるサルモネラ菌、腸炎ビブリオ菌および黄色ブドウ球菌についても同様に比較検討したところ、こんにゃくの液の抗菌効果は、O157と比べると同等かあるいはやや弱いようであった。しかし、これらの食中毒菌もこんにゃく液中では時間とともに減少し、2〜3日後ではほとんどが死滅することが判明した。さらに、市販こんにゃく液中の細菌の存在有無を検討したが、調べた限り、開封直後のこんにゃく液中には菌は検出されなかった。以上の結果から、こんにゃく液は衛生学的に安全性の高いことが証明され、静菌作用(菌の増殖抑制・阻害作用)というよりは、殺菌作用(生菌数の低減化作用)をもつことが明らかとなった。こんにゃくのような高アルカリ性呈示食品は、少数の生菌数の摂取でも発症するような腸管出血性大腸菌O157などの感染症防止に対しても、大きな役割を果たしているものと考えられる。

著者

Ahmad M. Al Athamneh. Supatta Chawalitpong 鈴木 湧太 山口 大樹 中村 宗一郎 片山 茂

出版者

Japanese Society of Food Chemistry

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.26, no.2, pp.91-98, 2019 (Released:2019-08-28)

参考文献数

24

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Fag e 1 is a 22 kDa globulin found in common buckwheat (Fagopyrum esculentum) and it is known to be one of the major allergens causing severe allergic symptoms. In this study, we successfully obtained recombinant Fag e 1 using the Pichia expression system and prepared an allergen-specific hypoallergenic agent by the controlled dry-heating phosphorylation of Fag e 1 (P-Fag e 1). Then, we investigated if P-Fag e 1 can be useful as an immunomodulator in Fag e 1-sensitized mice. For this, P-Fag e 1 was orally administrated into Fag e 1-sensitized mice for 6 weeks, and then these mice were challenged with Fag e 1. We observed a significant reduction in the histamine release in addition to diminished production of total as well as specific IgE in the P-Fag e 1-treated mice. In contrast, total IgA level increased by the treatment with P-Fag e1. The levels of the IL-4 cytokines from both spleen and Peyer’s patches were significantly decreased in P-Fag e 1 treated mice. Additionally, the population of T follicular helper cells (Tfh cells) was increased in the P-Fag e 1 treated group. The suppression of IgE production in the Fag e1 treated group might be due to the enrichment of the Tfh cells and IgA production. Therefore, it could be proposed that P-Fag e 1 is an allergen-specific immunomodulator in mice allergic to Fag e 1.

著者

三宅 義明 菅野 友美

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.25, no.2, pp.60-69, 2018

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The characteristics of flavonoids and coumarins in ten types of lemon (Lemon, Pink-lemonade, Meyer lemon, Ogasawarashimalemon, Hachijyo fruit lemon, Alen-eureka lemon, Lisbon, Vilafranca, Sweet lemon, Ponderosa) were examined. Twelveflavonoids and seven coumarins from the peel and juice of the different lemon types were analyzed using HPLC, and thequantitative values were subjected to a multivariate statistics principal component analysis. The ten lemon types were separatedinto four (I ~ IV) categories, corresponding to genus-species in plant classification (<i>Citrus limon</i>, <i>Citrus meyeri</i>, <i>Citrus limetta</i>,<i>Citrus pyriformis</i>), based on the scatter diagram of the score plot from the principal component analysis. Lemon, Pink-lemonade,Alen-eureka lemon, Lisbon, and Vilafranca were in category I: <i>C. limon</i>. Meyer lemon, Ogasawara-shima lemon, and Hachijyofruit lemon were in category II: <i>C. meyeri</i>. Sweet lemon (<i>C. limetta</i>) was in category III, and Ponderosa (<i>C. pyriformis</i>) was incategory IV. The characteristic flavonoids in the lemon types of category I were eriocitrin, diosmin, 6,8-C-diglucosylapigenin,and 6,8-C-diglucosyldiosmetin. Those of category III were narirutin, nobiletin, and tangeretin, and those of category IV wereneoeriocitrin, neohesperidin, and naringin. Coumarins were not found in the juices but were found in the peels. The characteristiccoumarins in the category I lemon types were 8-geranyloxypsolaren, 5-geranyloxypsolaren (bergamottin), and 5-geranyloxy-7-methoxycoumarin. That of the category II lemon types was 7-methoxy-5-prenyloxycoumarin, and that of the category IV types was7-geranyloxycoumarin (auraptene). The antioxidative activity for the peel extract of the category I lemon types was the highestactivity of all categories (p < 0.05) suggesting a relationship to the eriocitrin and neoeriocitrin content.

著者

田中 康雄 菊崎 泰枝 中谷 延二

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.9, no.2, pp.67-76, 2002

参考文献数

32

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精油50種類及びオレオレジン24種類について咽頭上気道細菌、<i>Streptococcus pyogenes</i>、<i>Haemophilus influenzae</i>、<i>Moraxella catarrhalis</i>に対する抗菌試験を行った。精油の中ではシナモンリーフ、セージ、クローブ、カルダモン、タイムに効果がみられ、オレオレジンではイチョウ、甘草、ナツメグに抗菌性が見られた。さらに代表的な精油成分より45種の揮発性化合物を選択し抗菌活性を調べた。精油成分の中ではcarvacrol、methyl chavicol、bornyl acetate、menthyl acetate、α-terpinyl acetate、viridiflorolに効果がみられた。カルダモン、ローレル、メリッサ精油と同様に3種の主要な成分geranyl acetate, menthyl acetate, α-terpinyl acetateは上気道微生物に対して有効な抗菌性成分となり得ることが示唆された。抗菌活性を示したシナモンリーフ油およびクローブ油の主成分eugenolは単独ではほとんど活性が認められなかったが、β-Caryophylleneに対してeugenolは優れた相乗効果を示すことが認められた。

著者

城戸 克己 廣瀬 恵美 片岡 裕美 増田 寿伸 田鶴谷(村山) 惠子

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.26, no.1, pp.68-76, 2019 (Released:2019-04-26)

参考文献数

18

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Many crude drug products elicit a bitter taste, because there are a variety of bitter components in them. However, there are no good methods of masking their bitter taste. Therefore, new masking methods are widely required. As a general method to administer crude drug products to patients, they are dissolved in hot water or mixed in food or juice. However, there is a risk that patients may not want to eat the food if the crude drug products alter the taste of the food. In this study, we conducted a sensory evaluation including a questionnaire in order to examine the improvement effect on swallowing a crude drug product in food. The screening tests were carried out to reveal what kinds of foods could improve the taste and texture of the crude drug product by mixing them with 30 kinds of foods. In the screening test, a statistically significant effect was observed on masking bitter taste. Based on this screening test, the bitterness masking tests were carried out with 6 kinds of foods. As a result, a commercially available swallowing aid jelly, vanilla ice cream, chocolate ice cream, condensed milk, peanut cream, and seaweed tsukudani significantly reduced the bitterness of the crude drug product. The tastes of these foods are strong, so it is necessary for patients with sugar and salinity limitations to consider the usage of these foods. These foods are relatively inexpensive and easy to obtain. Therefore, they might be useful for patients to take medicines such as bitter crude drug products following the instructions of a physician.

著者

石橋 正博 山田 傑 北村 尚男 真島 裕子 一色 賢司 伊藤 誉志男

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.2, no.2, pp.93-96, 1996-03-29 (Released:2017-12-01)

参考文献数

6

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縮合リン酸塩のピロリン酸、ポリリン酸、メタリン酸の1日摂取量をマーケットバスケット方式で調査した。加工食品約340品目を(1)調味嗜好飲料、(2)穀類、(3)イモ類・豆類・種実類、(4)魚介類・肉類、(5)油脂類・乳類、(6)佐藤類・菓子類、(7)果実類・野菜類・海草類の7群に分け、それぞれの群の縮合リン酸含有量を測定し、各群ことの喫食量をかけて摂取量とした。(1)縮合リン酸の1日摂取量は、15.8mgでピロリン酸が7.2mg、ポリリン酸が3.8mg、メタリン酸が5.0mgであった。(2)摂取量の多いのは、5群の6.0mgと4群の5.1mgで、主な摂取源は、5群のチーズと4群の魚介類・食肉類であった。特に、チーズの種類と喫食量は摂取量に大きく寄与することが分かった。(3)地区別の比較では、東部地区と西部地区がやや多かった。東部地区の5群が特に多かったのは、チーズの種類による影響と思われる。(4)昭和58年度、昭和62年度、平成3年度、平成6年度の調査結果より、縮合リン酸の摂取量は増加傾向にある。(5)世代別の摂取量の比較では、高齢者、学童が成人に比べ多くなった。4群は各世代に摂取量の違いはあまりなかったが、5群はチーズの種類の影響でかなりばらつきがあった。その他、学童の6群と7群も他の世代に比べやや摂取量が多かった。

著者

梁 佳 槌本 六秀 肖 寧 川口 夕貴 小野 要 濱田 友貴 谷山 茂人 橘 勝康

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.18, no.3, pp.168-173, 2011

参考文献数

20

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The ultrastructural changes with freshness decreasing of pink (P), white (W) and red (R) muscle in cultured carp were investigated during storage in ice in order to evaluate their ultrastructural characteristics and destruction after killing. The contents of ATP-related compounds in the three muscle types were higher in the order of W>P>R, and the increasing of K value was faster in the order of R>P>W. Destruction of zigzag structure of Z line, sarcoplasmic reticulum, and mitochondria was faster in the order of R>P>W. Disappearence of glycogen granule was also faster in the order of R>P>W. These results suggest that the interposition of pink muscle fibers into the dorsal ordinary muscle might accelerate freshness decreasing of fish.

著者

赤染 陽子 神田 智正 杉山 洋 大竹 康之 島崎 弘幸

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.14, no.2, pp.82-86, 2007-08-31

被引用文献数

1

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Apple polyphenols consisting of procyanidins as major components are extracted from apples (Rosaceae, Malus sp.). A double-blind within-subject crossover study was performed to assess the effects of apple polyphenol drink intake on postprandial serum triglyceride (TG) levels. Subjects were eleven adults with healthy volunteers (male/female =8/3, 35.6 ± 12.3 years of age, TG 30 - 149 mg/dl). Subjects had test drinks with 600 mg apple polyphenols or without apple polyphenols (placebo) with fat-rich meal (fat 40 g). Blood samples were taken before and 2, 3, 4, 6 hours after the food ingestion and serum TG levels were measured. Intake of apple polyphenol drink group significantly suppressed the level of TG observed at two hours after food ingestion as compared with that of the placebo (P<0.05) on nine analysis subjects in the normal range of the TG levels just before intake. These results suggested that intake of the apple polyphenols-supplemented drink suppresses postprandial serum TG levels in healthy subjects.

著者

久城 真代 鄭 雅志 Thammawong Manasikan 小澤 徹 中川 博之 長嶋 等 岡留 博司

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.21, no.3, pp.173-178, 2014

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ゼアラレノン(ZEA)は小麦の主要な自然汚染物質であり、同じくフザリウム属菌産生カビ毒の一種であるデオキシニバレノール(DON)との共汚染が起こりやすい。小麦加工後のZEAの残存率を明らかにし、DONの残存率と比較するため、人工的にフザリウム属菌に罹病させた国産軟質小麦を用いて製粉を行い、小麦半加工製品(小麦粉)でのZEAの濃度変化を解析した。穀粒を製粉に供し、3種類のブレーキ粉(1B、2B、3B)、3種類のミドリング粉(1M、2M、3M)ならびに外皮分画(大ふすま、小ふすま)を得た。ストレート粉は6種全ての粉から調製し、ヒトの可食部となる上質粉は1B、1M、2Bと2Mから調製した。単一試験室で妥当性確認された、多機能カートリッジ精製とHPLC蛍光検出を用いる分析法にて、ストレート粉、上質粉、大ふすま、小ふすまに含まれるZEAを定量した。DONについても同様に定量を行った。ストレート粉におけるZEAの残存率は50%未満であり、DONの残存率より低かった。上質粉でのZEAの残存率はさらに低く、粉の分画によりZEAが効率的に減衰することが示された。

著者

渕野 裕之 福井 ななみ 飯田 修 和田 浩志 川原 信夫

出版者

Japanese Society of Food Chemistry

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.25, no.3, pp.152-159, 2018 (Released:2018-12-21)

参考文献数

35

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The inhibitory effect of Kaempferia parviflora (black ginger) constituents on nitric oxide (NO) production was examined. From the methanolic extract of K. parviflora rhizome, 16 flavones, 14 flavanones, 2 diarylheptanoids, 2 chalcones and a stilbene were isolated and their inhibitory activities toward NO production were examined. As a result, two methoxyflavones, 5,7,3’,4’-tetramethylluteolin and 5,7-dimethoxyflavone showed significant activities. Most of the flavanone constituents, except for 5,7,3’-trimethoxy-4’-hydroxyflavanone, showed lower activities. The most potent active constituent of Black ginger was the chalcone flavokavin B; its derivative, flavokavin A, also showed significant activity. The curcumin and its derivative, 1-(4-hydroxyphenyl)-7-phenyl-1,6-heptadiene-3,5-dione, showed significant activity. Only one stilbene was isolated, and it showed low activity.

著者

藤井 優寿 宮地 夏海 常松 順子 中牟田 啓子

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.25, no.2, pp.92-96, 2018

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A method for analysis of okadaic acid (OA), dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) using deproteinationcartridge filter was developed. This is a simple method because it does not require complicated operations such as conditioningand washing of the column. The shellfish poisons in oyster were extracted with methanol and methanol/water (9:1), and werehydrolyzed with 2.5 mol/L sodium hydroxide solution. The crude extracts were defatted by liquid- liquid partition using n-hexane,and were passed through a deproteination cartridge filter. Measurement was performed by liquid chromatography tandem massspectrometry. As a result of the validation study using the matrix calibration curve method, the trueness, repeatability and withinlaboratoryreproducibility were 92-98, 1.0-3.0 and 2.0-4.0%, respectively, and met the target values of notification of Ministry ofHealth, Labour and Welfare of Japan.