著者

近藤 三雄

出版者

日本芝草学会

雑誌

芝草研究 (ISSN:02858800)

巻号頁・発行日

vol.34, no.2, pp.33-34, 2006

著者

田沢裕賀研究代表 東京国立博物館編集

出版者

東京国立博物館

巻号頁・発行日

2016

著者

久我 創紀 中村 文一 佐藤 康之

出版者

自動制御連合講演会

雑誌

自動制御連合講演会講演論文集

巻号頁・発行日

vol.57, pp.1227-1230, 2014

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V-22オスプレイや4回転翼型飛行ロボットの位置,姿勢制御モデルであるPVTOLシステムは難しい制御特性を持つ.先行研究においてPVTOLシステムを安定化させる静的な厳密制御Lyapunov関数(SCLF)を設計し,逆最適制御則を設計した.しかしながら,この制御則は,システムのパラメーターの不確かさに対しロバストでない.そこで本研究では,システムのパラメータの不確かさに対してゲイン余裕を保証する適応制御則を提案する.加えて,コンピュータシミュレーションによって提案法の有効性を示す.

著者

青木 繁

出版者

日本演劇学会

雑誌

演劇学論集 日本演劇学会紀要 (ISSN:13482815)

巻号頁・発行日

vol.25, pp.45-54, 1987-04-10 (Released:2020-12-14)

著者

玉田玉秀斎 講演

出版者

樋口隆文館

巻号頁・発行日

1909

著者

Kalimullah Saighani Daiki Kondo Naoto Sano Kazumasa Murata Tetsuya Yamada Motoki Kanekatsu

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.277-283, 2021-06-25 (Released:2021-06-25)

参考文献数

34

被引用文献数

8

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The mature embryos of rice seeds contain translatable mRNAs required for the initial phase of germination. To clarify the relationship between seed longevity and RNA integrity in embryos, germinability and stability of embryonic RNAs were analyzed using the seeds of japonica rice cultivars subjected to controlled deterioration treatment (CDT) or long periods of storage. Degradation of RNA from embryos of a japonica rice cultivar “Nipponbare” was induced by CDT before the decline of the germination rate and we observed a positive relationship between seed germinability and integrity of embryonic RNAs. Moreover, this relationship was confirmed in the experiments using aged seeds from the “Nipponbare”, “Sasanishiki” and “Koshihikari” rice cultivars. In addition, the RNA integrity number (RIN) values, calculated using electrophoresis data and Agilent Bioanalyzer software, had a positive correlation with germinability (R2=0.75). Therefore, the stability of embryonic RNAs required for germination is involved in maintaining seed longevity over time and RIN values can serve as a quantitative indicator to evaluate germinability in rice.

著者

Hirotomo Takatsuka Yuji Nomoto Satoshi Araki Yasunori Machida Masaki Ito

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.269-275, 2021-06-25 (Released:2021-06-25)

参考文献数

21

被引用文献数

4

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MYB3R family transcription factors play a central role in the regulation of G2/M-specific gene transcription in Arabidopsis thaliana. Among the members of this family, MYB3R3 and MYB3R5 are structurally closely related and are involved in the transcriptional repression of target genes in both proliferating and quiescent cells. This type of MYB3R repressor is widespread in plants; however, apart from the studies on MYB3Rs in Arabidopsis thaliana, little information about them is available. Here we isolated tobacco cDNA clones encoding two closely related MYB3R proteins designated as NtmybC1 and NtmybC2 and determined the nucleotide sequences of the entire coding regions. Phylogenetic analysis suggested that NtmybC1 and NtmybC2 can be grouped into a conserved subfamily of plant MYB3Rs that also contains MYB3R3 and MYB3R5. When transiently expressed in protoplasts prepared from tobacco BY-2 cells, NtmybC1 and NtmybC2 repressed the activity of target promoters and blocked promoter activation mediated by NtmybA2, a MYB3R activator from tobacco. Unlike MYB3R3 and MYB3R5, NtmybC1 and NtmybC2 showed cell cycle-regulated transcript accumulation. In synchronized cultures of BY-2 cells, mRNAs for both NtmybC1 and NtmybC2 were preferentially expressed during the G2 and M phases, coinciding with the expression of NtmybA2 and G2/M-specific target genes. These results not only broadly confirm our fundamental view that this type of MYB3R protein acts as transcriptional repressor of G2/M-specific genes but also suggest a possible divergence of MYB3R repressors in terms of the mechanisms of their action and regulation.

著者

Kazusato Oikawa Takuto Imai Yutaka Kodama Keiji Numata

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.257-262, 2021-06-25 (Released:2021-06-25)

参考文献数

15

被引用文献数

2

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Mitochondria-selective fluorescent probes such as MitoTracker are often used for mitochondria imaging in various plants. Although some of the probes are reported to induce mitochondria dysfunction in animal cells, the effect on plant cells remains to be determined. In the present study, we applied quantitative methods to analyze mitochondrial movement, speed frequency, and speed-angle changes, based on trajectory analysis of mitochondria in mesophyll protoplast cells of Arabidopsis thaliana expressing the mitochondria-localized fluorescent protein. Using the quantitative method, we assessed whether MitoTracker Red (FM and CMXRos) induce mitochondria dysfunction in A. thaliana. Although both the fluorescent probes well-stained mitochondria, the CMXRos probe, not the FM probe, gave a severe effect on mitochondrial movement at the low concentration (10 nM), indicating a MitoTracker-induced mitochondria dysfunction in A. thaliana. These results revealed that our quantitative method based on mitochondrial movement can be used to determine the appropriate concentrations of mitochondria-selective fluorescent probes in plants.

著者

砂原 庸介

出版者

中央公論新社

雑誌

中央公論 (ISSN:05296838)

巻号頁・発行日

vol.131, no.10, pp.64-71, 2017-10

著者

Wiluk Chacuttayapong Harumi Enoki Yusei Nabetani Minami Matsui Taichi Oguchi Reiko Motohashi

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.247-256, 2021-06-25 (Released:2021-06-25)

参考文献数

58

被引用文献数

3

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The development of green energy is important to mitigate global warming. Jatropha (Jatropha curcas L.) is a promising candidate for the production of alternative biofuel, which could reduce the burden on the Earth’s resources. Jatropha seeds contain a large quantity of lipids that can be used to produce biofuel, and the rest of the plant has many other uses. Currently, techniques for plant genetic transformation are extensively employed to study, create, and improve the specific characteristics of the target plant. Successful transformation involves the alteration of plants and their genetic materials. The aim of this study was to generate Jatropha plants that can support biofuel production by increasing their seed size using genes found via the rice FOX-hunting system. The present study improved previous protocols, enabling the production of transgenic Jatropha in two steps: the first step involved using auxins and dark incubation to promote root formation in excised shoots and the second step involved delaying the timing of antibiotic selection in the cultivation medium. Transgenic plants were subjected to PCR analysis; the transferred gene expression was confirmed via RT-PCR and the ploidy level was investigated. The results suggest that the genes associated with larger seed size in Arabidopsis thaliana, which were found using the rice FOX-hunting system, produce larger seeds in Jatropha.

著者

Takeshi Matsui Eiji Takita Seika Oiwa Asuka Yokoyama Ko Kato Kazutoshi Sawada

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.239-246, 2021-06-25 (Released:2021-06-25)

参考文献数

47

被引用文献数

2

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Plant-made oral vaccines can be a cost-effective method to control infectious diseases of humans and farm animals. Pig edema is a bacterial disease caused by enterohemorrhagic Escherichia coli producing the toxin Shiga toxin 2e (Stx2e). In our previous report, we chose the non-toxic B subunit of Stx2e (Stx2eB) as a vaccine antigen, and Stx2eB was expressed in lettuce (Lactuca sativa L., cv. Green wave). We found that a double repeated Stx2eB (2×Stx2eB) accumulates to higher levels than a single Stx2eB. In this study, we analyzed progeny plants introduced with 2×Stx2eB in which the gene was expressed under the control of conventional cauliflower mosaic virus 35S RNA (CaMV 35S) promoter, and found that the lettuce underwent transgene silencing and bore few seeds. We resolved these problems by using a transgene cassette which harbored a transcriptional promoter derived from the lettuce ubiquitin gene and a longer version of HSPT. The lettuce harboring this expression construct will be valuable in establishing the seed lot system on the basis that thousands of seeds can be obtained from one plant body and the resulting progeny plants accumulate 2×Stx2eB at high levels without the transgene silencing.

著者

Marcos Fernando Basso Karoline Estefani Duarte Thais Ribeiro Santiago Wagner Rodrigo de Souza Bruno de Oliveira Garcia Bárbara Dias Brito da Cunha Adilson Kenji Kobayashi Hugo Bruno Correa Molinari

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.227-238, 2021-06-25 (Released:2021-06-25)

参考文献数

64

被引用文献数

8

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The CRISPR/Cas9 system has been used for genome editing in several organisms, including higher plants. This system induces site-specific mutations in the genome based on the nucleotide sequence of engineered guide RNAs. The complex genomes of C4 grasses makes genome editing a challenge in key grass crops like maize (Zea mays), sorghum (Sorghum bicolor), Brachiaria spp., switchgrass (Panicum virgatum), and sugarcane (Saccharum spp.). Setaria viridis is a diploid C4 grass widely used as a model for these C4 crop plants. Here, an optimized CRISPR/Cas9 binary vector that exploits the non-homologous end joining (NHEJ) system was used to knockout a green fluorescent protein (gfp) transgene in S. viridis accession A10.1. Transformation of embryogenic callus by A. tumefaciens generated ten glufosinate-ammonium resistant transgenic events. In the T0 generation, 60% of the events were biallelic mutants in the gfp transgene with no detectable accumulation of GFP protein and without insertions or deletions in predicted off-target sites. The gfp mutations generated by CRISPR/Cas9 were stable and displayed Mendelian segregation in the T1 generation. Altogether, the system described here is a highly efficient genome editing system for S. viridis, an important model plant for functional genomics studies in C4 grasses. Also, this system is a potential tool for improvement of agronomic traits in C4 crop plants with complex genomes.

著者

國田 祥子 小阪 芙由美 西 菜見子

出版者

中国学園大学/中国短期大学

雑誌

中国学園紀要 = Journal of Chugokugakuen (ISSN:13479350)

巻号頁・発行日

no.18, pp.113-122, 2019-06-16

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近年,待機児童の問題が大きく取り上げられている。保育施設の増加が必要だが,保育士不足もあって容易ではない。保育士不足の原因の1つに,早期離職者の多さがあると言われている。そこで本研究では,保育士がなぜ早期に離職してしまうのかを検討する。 保育士146名に質問紙調査を行い,離職を考えた経験とその理由,職場環境について尋ねた。その結果,20代後半以降や保育経験6年以上で離職を考えた経験のある人が増加していた。離職を考えた理由は「職場の人間関係」「残業が多い」「給与面」が,保育士を続けてきてよかったことは「子どもの成長に貢献できていると思うこと」「子どもと一緒に過ごせること」「保育を通して充実感が得られること」が多かった。また離職を考えた経験のない人は,ある人よりも現在の職場には研修機会が多く,相談相手がいると感じていた。 保育士の多くは職場の人間関係や待遇に不満を感じつつも,自分の保育を高める機会や相談できる同僚の存在に支えられ,子どもの成長を糧として仕事を続けていることが示唆された。多様な価値観を許容する職場風土の醸成や保育士の待遇改善が,早期離職者を減らすために有効なのではないだろうか。

著者

太田 朋子

出版者

学士会

雑誌

学士会会報

巻号頁・発行日

vol.2015, no.4, pp.65-68, 2015-07

著者

髙平 妙心

出版者

日本印度学仏教学会

雑誌

印度學佛教學研究 (ISSN:00194344)

巻号頁・発行日

vol.56, no.2, pp.668-671, 2008

著者

金子淳一著

出版者

新潮社

巻号頁・発行日

2009

出版者

宝島社

巻号頁・発行日

2005

出版者

一般社団法人 日本鉱物科学会

雑誌

岩石鉱物科学 (ISSN:1345630X)

巻号頁・発行日

vol.49, no.4, pp.148, 2021

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<p>黒田吉益先生のご逝去を悼む</p>

著者

黒田 吉益

出版者

日本地質学会

雑誌

地質学雑誌 (ISSN:00167630)

巻号頁・発行日

vol.96, no.9, pp.775-786, 1990

著者

黒田 吉益

出版者

東京教育大学

巻号頁・発行日

1960

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博士論文