著者

冨樫 玖苑 小林 正樹

出版者

電気関係学会東北支部連合大会実行委員会

雑誌

電気関係学会東北支部連合大会講演論文集 平成26年度 電気関係学会東北支部連合大会 講演論文集

巻号頁・発行日

pp.165, 2014 (Released:2014-11-04)

著者

齋藤 剛 浅井 こずえ 日下部 真理 前田 恒昭 衣笠 晋一

出版者

公益社団法人 日本化学会・情報化学部会

雑誌

ケモインフォマティクス討論会予稿集 第26回情報化学討論会

巻号頁・発行日

pp.JP22, 2003 (Released:2003-10-25)

参考文献数

2

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有機化合物のスペクトルデータベース(SDBS)を維持・向上するために,データ入力ツールを整備することは重要である.データ入力用のツールを作成したが,SDBSのデータ量に不向きであったため、これを向上したので報告する.

著者

長井 勝利

出版者

公益社団法人 高分子学会

雑誌

高分子 (ISSN:04541138)

巻号頁・発行日

vol.33, no.9, pp.686-689, 1984-09-01 (Released:2011-10-14)

参考文献数

31

著者

藤原 鎮男

出版者

公益社団法人 高分子学会

雑誌

高分子 (ISSN:04541138)

巻号頁・発行日

vol.6, no.6, pp.302-306, 1957-05-20 (Released:2011-10-11)

参考文献数

43

著者

熨斗 秀夫 小林 昇二 山田 照幸

出版者

繊維学会

雑誌

繊維学会誌 (ISSN:00379875)

巻号頁・発行日

vol.33, no.9, pp.T470-T477, 1977-09-10 (Released:2008-11-28)

参考文献数

3

被引用文献数

1

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The sensory value for rhythm according to moving skirt were obtained by sensory test. The moving type of skirt is circular arc-reciprocation and skirt is dressed on the nude damy.The obtained results are as follows.(1) The relation between the moving type and sensory value for rhythm.(a) When the velocity of reciprocation is about 40cycle/min, the maximum sensory value for rhythm on each arc angle is obtained, in case of range of arc angle 30°C-170°C.(b) The maximum sensory values are classified into two groups. In the first group, the sensory values on each arc angle increase with increasing the arc angle, and in the second group, those become nearly same in spite of the arc angle.(2) The effect of the kinds of cloth and the constructive factors on skirt for the sensory value of rhythm.(a) In case the train-circumference of skirt is long, the sensory value becomes large.(b) The length of skirt gives little effect on the sensory value.(c) The properties of cloth give a little effect on the sensory value.In case both the length and the train-circumference of skirt are long, the sensory value under thick cloth becomes large, and in case both are short, the sensory value under thin cloth becomes large on the contrary.(d) The sensory value of rhythm becomes large, in case the node on skirt is deep.

著者

松村 敦 濱沖 肯志郎 榎本 祐季 三島 悠希

出版者

情報知識学会

雑誌

情報知識学会誌 (ISSN:09171436)

巻号頁・発行日

vol.26, no.2, pp.211-216, 2016-05-14 (Released:2016-07-15)

参考文献数

5

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子どもの質問には,その子どもの興味が表れることに着目して,登録された質問に人手で絵本を推薦するソーシャル絵本推薦システム「ぴくぶく」を開発している.本稿では,「ぴくぶく」に自動推薦機能を追加することで,絵本の推薦率を向上させ,活発な絵本推薦を促すことを試みた.14組の親子を対象とした利用実験を行った結果を報告する.

著者

榎本 翔

出版者

情報知識学会

雑誌

情報知識学会誌 (ISSN:09171436)

巻号頁・発行日

vol.26, no.2, pp.221-226, 2016-05-14 (Released:2016-07-15)

参考文献数

12

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近年,電子媒体の資料が増加している.研究者が資料を入手できるように,リンクリゾルバーやベンダーによるウェビナーの開催といった工夫が多く見られる.一方,大学図書館ではデータベースの代理検索や利用教育を行ってきたが,最新の動向は明らかになっていない.本研究では,日本の大学図書館におけるデータベース利用案内と利用教育がどのように行われているのか,複数の大学を抽出しWebサイトを通じて調査をし分析を行った.その結果,設置母体や大学の規模によって利用案内や利用教育の内容量に差が見られた.

著者

今西 美音子 佐野 友紀

出版者

日本建築学会

雑誌

日本建築学会計画系論文集 (ISSN:13404210)

巻号頁・発行日

vol.79, no.698, pp.917-922, 2014-04-30 (Released:2014-07-15)

参考文献数

23

被引用文献数

2 5

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This study analyzes the pedestrian's avoidance behavior in crossing flow in order to obtain quantitative criteria for evaluating the difficulty of walking in a crowd. The new graphic illustration methods, called Short-Time Pedestrian Path Diagram and Direction Rose Diagram, are developed and proposed in this paper to represent the state of a crowd visually. The result of our experiment suggests that pedestrians descend their walking speed or/and detour to avoid striking other people and each avoidance behavior can be classified into three levels, whose threshold are defined from the speed or the angular velocity of each pedestrian.

著者

佐藤 一省 前田 法史 小川 範之

出版者

公益社団法人 化学工学会

雑誌

化学工学論文集 (ISSN:0386216X)

巻号頁・発行日

vol.21, no.3, pp.632-635, 1995-05-10 (Released:2009-11-12)

参考文献数

3

被引用文献数

1 1

---

The minimum power of impeller required for complete suspension of solid particles, based on Zwietering's criterion, was measured for single and twin impeller systems. The effects of the configuration of vessel and the number of impellers on the minimum required power were examined.The minimum required power per unit liquid volume Pfv for a single impeller system increased gradually as the length ratio of long side to short side A/B incresed over 1.5 Pfv for a twin impeller system remained nearly constant at a lower value than that of a single impeller system in the range of A/B=1.52.25.The Pfv of a pitched-blade turbine was below 1 / 2 of the value of a flat-blade disc turbine under the same conditions and was independent of the rotational direction of each impeller.

著者

Oanh T. P. Kim Manh D. Le Hoang X. Trinh Hai V. Nong

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.173-180, 2016 (Released:2016-07-14)

参考文献数

42

被引用文献数

22

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Tumor necrosis factor-alpha (TNF-α) is a cytokine that plays an important role in inflammatory process and tumor development. Recent studies demonstrate that triterpene saponins from Vietnamese ginseng are efficient inhibitors of TNF-α. But the interactions between TNF-α and the saponins are still unclear. In this study, molecular docking and molecular dynamics simulations of TNF-α with three different triterpene saponins (majonoside R2, vina-ginsenoside R1 and vina-ginsenoside R2) were performed to evaluate their binding ability. Our results showed that the triterpene saponins have a good binding affinity with protein TNF-α. The saponins were docked to the pore at the top of the “bell” or “cone” shaped TNF-α trimer and the complexes were structurally stable during 100 ns molecular dynamics simulation. The predicted binding sites would help to subsequently investigate the inhibitory mechanism of triterpene saponins.

著者

Hiroo Imai Nami Suzuki-Hashido Yoshiro Ishimaru Takanobu Sakurai Lijie Yin Wenshi Pan Masaji Ishiguro Katsuyoshi Masuda Keiko Abe Takumi Misaka Hirohisa Hirai

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.165-171, 2016 (Released:2016-07-14)

参考文献数

14

被引用文献数

1 5

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In mammals, bitter taste is mediated by TAS2Rs, which belong to the family of seven transmembrane G protein-coupled receptors. Since TAS2Rs are directly involved in the interaction between mammals and their dietary sources, it is likely that these genes evolved to reflect species-specific diets during mammalian evolution. Here, we analyzed the amino acids responsible for the difference in sensitivities of TAS2R16s of various primates using a cultured cell expression system. We found that the sensitivity of TAS2R16 varied due to several amino acid residues. Mutation of amino acid residues at E86T, L247M, and V260F in human and langur TAS2R16 for mimicking the macaque TAS2R16 decreased the sensitivity of the receptor in an additive manner, which suggests its contribution to the potency of salicin, possibly via direct interaction. However, mutation of amino acid residues 125 and 133 in human TAS2R16, which are situated in helix 4, to the macaque sequence increased the sensitivity of the receptor. These results suggest the possibility that bitter taste sensitivities evolved independently by replacing specific amino acid residues of TAS2Rs in different primate species to adapt to species-specific food.

著者

Kazunori D. Yamada Hafumi Nishi Junichi Nakata Kengo Kinoshita

出版者

The Biophysical Society of Japan

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.157-163, 2016 (Released:2016-07-14)

参考文献数

41

被引用文献数

7

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Functional sites on proteins play an important role in various molecular interactions and reactions between proteins and other molecules. Thus, mutations in functional sites can severely affect the overall phenotype. Progress of genome sequencing projects has yielded a wealth of information on single nucleotide variants (SNVs), especially those with less than 1% minor allele frequency (rare variants). To understand the functional influence of genetic variants at a protein level, we investigated the relationship between SNVs and protein functional sites in terms of minor allele frequency and the structural position of variants. As a result, we observed that SNVs were less abundant at ligand binding sites, which is consistent with a previous study on SNVs and protein interaction sites. Additionally, we found that non-rare variants tended to be located slightly apart from enzyme active sites. Examination of non-rare variants revealed that most of the mutations resulted in moderate changes of the physico-chemical properties of amino acids, suggesting the existence of functional constraints. In conclusion, this study shows that the mapping of genetic variants on protein structures could be a powerful approach to evaluate the functional impact of rare genetic variations.

著者

Masayuki Oda Takeshi Tsumuraya Ikuo Fujii

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.135-138, 2016 (Released:2016-07-14)

参考文献数

14

被引用文献数

2

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We analyzed the correlation between the conformational strain and the binding kinetics in antigen-antibody interactions. The catalytic antibodies 6D9, 9C10, and 7C8 catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. The crystal structure of 6D9 complexed with a transition-state analog (TSA) suggests that 6D9 binds the substrate to change the conformation of the ester moiety to a thermodynamically unstable twisted conformation, enabling the substrate to reach the transition state during catalysis. The present binding kinetic analysis showed that the association rate for 6D9 binding to the substrate was much lower than that to TSA, whereas those for 9C10 and 7C8 binding were similar to those to TSA. Considering that 7C8 binds to the substrate with little conformational change in the substrate, the slow association rate observed in 6D9 could be attributed to the conformational strain in the substrate.

著者

Yuka Suzuki Kei Yura

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.127-134, 2016 (Released:2016-07-14)

参考文献数

26

被引用文献数

4

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We investigated the effect of ATP binding to GroEL and elucidated a role of ATP in the conformational change of GroEL. GroEL is a tetradecamer chaperonin that helps protein folding by undergoing a conformational change from a closed state to an open state. This conformational change requires ATP, but does not require the hydrolysis of the ATP. The following three types of conformations are crystalized and the atomic coordinates are available; closed state without ATP, closed state with ATP and open state with ADP. We conducted simulations of the conformational change using Elastic Network Model from the closed state without ATP targeting at the open state, and from the closed state with ATP targeting at the open state. The simulations emphasizing the lowest normal mode showed that the one started with the closed state with ATP, rather than the one without ATP, reached a conformation closer to the open state. This difference was mainly caused by the changes in the positions of residues in the initial structure rather than the changes in “connectivity” of residues within the subunit. Our results suggest that ATP should behave as an insulator to induce conformation population shift in the closed state to the conformation that has a pathway leading to the open state.

著者

Georgios Iakovou Stephen Laycock Steven Hayward

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.97-103, 2016 (Released:2016-07-14)

参考文献数

37

被引用文献数

5

---

Interactive haptics-assisted docking provides a virtual environment for the study of molecular complex formation. It enables the user to interact with the virtual molecules, experience the interaction forces via their sense of touch, and gain insights about the docking process itself. Here we use a recently developed haptics software tool, Haptimol_RD, for the rigid docking of protein subunits to form complexes. Dimers, both homo and hetero, are loaded into the software with their subunits separated in space for the purpose of assessing whether they can be brought back into the correct docking pose via rigid-body movements. Four dimers were classified into two types: two with an interwinding subunit interface and two with a non-interwinding subunit interface. It was found that the two with an interwinding interface could not be docked whereas the two with the non-interwinding interface could be. For the two that could be docked a “sucking” effect could be felt on the haptic device when the correct binding pose was approached which is associated with a minimum in the interaction energy. It is clear that for those that could not be docked, the conformation of one or both of the subunits must change upon docking. This leads to the steric-based concept of a locked or non-locked interface. Non-locked interfaces have shapes that allow the subunits to come together or come apart without the necessity of intra-subunit conformational change, whereas locked interfaces require a conformational change within one or both subunits for them to be able to come apart.

著者

Kentaro Ishii Masanori Noda Susumu Uchiyama

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.87-95, 2016 (Released:2016-07-14)

参考文献数

32

被引用文献数

32

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The interactions of small molecules with proteins (protein–ligand interactions) mediate various biological phenomena including signal transduction and protein transcription and translation. Synthetic compounds such as drugs can also bind to target proteins, leading to the inhibition of protein–ligand interactions. These interactions typically accompany association–dissociation equilibrium according to the free energy difference between free and bound states; therefore, the quantitative biophysical analysis of the interactions, which uncovers the stoichiometry and dissociation constant, is important for understanding biological reactions as well as for rational drug development. Mass spectrometry (MS) has been used to determine the precise molecular masses of molecules. Recent advancements in MS enable us to determine the molecular masses of protein–ligand complexes without disrupting the non-covalent interactions through the gentle desolvation of the complexes by increasing the vacuum pressure of a chamber in a mass spectrometer. This method is called MS under non-denaturing conditions or native MS and allows the unambiguous determination of protein–ligand interactions. Under a few assumptions, MS has also been applied to determine the dissociation constants for protein–ligand interactions. The structural information of a protein–ligand interaction, such as the location of the interaction and conformational change in a protein, can also be analyzed using hydrogen/deuterium exchange MS. In this paper, we briefly describe the history, principle, and recent applications of MS for the study of protein–ligand interactions.

著者

滝沢 佳郎 畑 俊彦 遠藤 芳秀

出版者

一般社団法人 日本鉄鋼協会

雑誌

鉄と鋼 (ISSN:00211575)

巻号頁・発行日

vol.70, no.16, pp.2301-2304, 1984-12-01 (Released:2009-06-30)

参考文献数

8

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Determination of free nitrogen in steel by constant temperature-hydrogen hot extraction method was studied and optimum analytical conditions were established.1) Lower measured values were obtained with samples that had passed for long time after powdering. 2) The result of 1) was considered to come from the fact that dissolved nitrogen in steel was locked to dislocations and/or formed cluster gradually, thus becoming difficult to be extracted. 3) Higher temperature of environment around samples accelerated these effects, so it must be avoided to heat powdered samples for washing and drying. 4) It is necessary to analyse samples of finer powders soon after powdering. 5) When the sample was analysed under these analytical conditions, free nitrogen could be fully extracted at 400°C.

出版者

日本魚類学会

雑誌

魚類学雑誌 (ISSN:00215090)

巻号頁・発行日

vol.43, no.1, pp.50-57, 1996-05-31 (Released:2010-06-28)

著者

Jung-Sub Shin Hee-Won Park Gyo In Hyun Kyu Seo Tae Hyung Won Kyoung Hwa Jang Byung-Goo Cho Chang Kyun Han Jongheon Shin

出版者

公益社団法人日本薬学会

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

pp.c16-00240, (Released:2016-07-05)

参考文献数

17

被引用文献数

14

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Panax ginseng C.A. Meyer is one of the most popular medicinal herbs in Asia and the chemical constituents are changed by processing methods such as steaming or sun drying. Metabolomic analysis was performed to distinguish age discrimination of four- and six-year-old red ginseng using ultra-performance liquid chromatography quadruple time of flight mass spectrometry (UPLC-QToF-MS) with multivariate statistical analysis. Principal component analysis (PCA) showed clear discrimination between extracts of red ginseng of different ages and suggest totally six discrimination markers (two for four-year-old and four for six-year-old red ginseng). Among these, one marker was isolated and the structure determined by NMR spectroscopic analysis was 13-cis-docosenamide (marker 6-1) from six-year-old red ginseng. This is the first report of a metabolomic study regarding the age differentiation of red ginseng using UPLC-QToF-MS and determination of the structure of the marker. These results will contribute to the quality control and standardization as well as provide a scientific basis for pharmacological research on red ginseng.

著者

シュプリンガー マークS. 樋口 広芳 上田 恵介 ミントン ジェイソン シブレイ チャールズG.

出版者

Yamashina Institute for Ornitology

雑誌

山階鳥類研究所研究報告 (ISSN:00440183)

巻号頁・発行日

vol.27, no.2, pp.66-77_1, 1995-10-30 (Released:2008-11-10)

参考文献数

37

被引用文献数

8 8

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小笠原諸島の固有種,メグロApalopteron familiareは,1958年以降ミツスイ科に分顔されているが,これまでにヒヨドリ科,メジロ科,チメドリ科などにも分類されており,分類学上の位置が不安定な鳥である.われわれはミトコンドリア12SリボソームRNAをコードするDNAの塩基配列に基づく分子生物学的手法により,メグロがメジロ科の1種であることを示す証拠を得た.また,メジロ科の中では,マリアナ諸島南部に生息するオウゴンメジロCleptornis marcheiに近縁であることを明らかにした.オウゴンメジロもかつてはミツスイ類とされていたが,最近,DNA-DNAハイブリダイゼーション法や生態•行動研究などによってメジロ類であることが判明した鳥である.メグロは,相互羽づくろいや接触就眠を行なう,巣づくりから育雛まで雌雄で行なう,求愛給餌を行なわない,などの生態,行動面でもメジロ類に似ている.また小笠原諸島には元来メジロ類は分布しないことになっているが,メグロがメジロ類の1種になることで分布のこの奇妙な空白も埋まることになる.