著者

Qin-Fan LI Cai-Ju HAO Yong-Ping XU Jie LIANG Kai YANG Zhong-Hua CUI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1203220797, (Released:2012-03-26)

被引用文献数

5 8

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By a series of experiments, we identified a new member of locoweed family, Oxytropis serioopetala, which produces swainsonine, a phytotoxin harmful to livestock. In order to evaluate the toxicity of Oxytropis serioopetala, its extract was administered to ten rabbits by gavage at a dose of 1.5 mg/kg body weight as swainsonine once daily. After the 20th day, the rabbits appeared depressive and anorexia. Besides, intention tremors were apparent upon move. Their eyes were dull. The rear limbs were severely weak and even progressed to partial paresis. Activities of serum aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (AKP) and urea nitrogen (BUN) levels in the poisoned rabbits increased significantly. Serum α-mannosidase (AMA) activity decreased markedly. Pathomorphological lesions in the locoweed-poisoned rabbits developed severe microvacuolation of visceral and neurological tissue. The extensive vacuolation was observed in liver, kidney and brain. These clinical and pathological features are similar to the symptoms of locoism.

著者

廣井 準也

出版者

日本比較内分泌学会

雑誌

日本比較内分泌学会ニュース (ISSN:09139044)

巻号頁・発行日

vol.2002, no.106, pp.106_27-106_29, 2002 (Released:2002-08-26)

参考文献数

6

著者

Kenji Nakai Junichi Tsuboi Hitoshi Okabayashi Akimune Fukushima Manabu Itoh Hiroshi Kawata Fumihiko Miyake Hiroshi Kasanuki

出版者

International Heart Journal Association

雑誌

International Heart Journal (ISSN:13492365)

巻号頁・発行日

vol.48, no.6, pp.701-713, 2007 (Released:2007-12-27)

参考文献数

23

被引用文献数

15 18 13

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Noninvasive risk stratification is important for screening for lethal arrhythmia. We developed a 187-channel signal-averaged vector-projected high-resolution electrocardiograph (187-ch SAVP-ECG) for detecting abnormalities in the spatial location of ventricular high-frequency late potentials (HFLPs) and ventricular repolarization. The subjects consisted of 30 normal controls (CONTROL) and 13 patients with HFLPs (6 with myocardial infarction [MI], 6 with cardiomyopathy, and 1 with Brugada syndrome). The modified X, Y, Z-lead ECG and the synthesized signals from vector-projected 187-channel ECGs were amplified and passed through a digital filter. We calculated the integration of the HFLPs area between QRSend and 30 ms before QRSend. The integrated HFLPs map was superimposed on the corrected recovery time (RTc) and Tpeak-end dispersion maps composed by 187-ch SAVP-ECG. All patients received an examination by 64-channel magnetocardiography (64-ch MCG) on the same day. The spatial distribution of HFLPs by the 187-ch SAVP-ECG map was in agreement with the location of increased RT dispersion in MI. The spatial distribution of HFLPs in DCM demonstrated a wide variety of patterns. Interestingly, the spatial distribution of HFLPs in cases with ARVC was located at around a right ventricular outflow region. The spatial distribution of HFLPs by 187-ch SAVP-ECG was in agreement with those determined by 64-ch MCG. The 187-ch SAVP-ECG might be useful for evaluating the spatial distribution of nonuniform conduction and ventricular repolarization heterogeneity.

著者

Toshiya MASUDA Nao FUJITA Yuka ODAKA Yoshio TAKEDA Shigetomo YONEMORI Katsuo NAKAMOTO Hideki KUNINAGA

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.71, no.9, pp.2316-2320, 2007-09-23 (Released:2007-09-23)

参考文献数

12

被引用文献数

15

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The tyrosinase inhibitory activity of the extracts of 53 parts of 36 plant species cultivated for edible and medicinal use in Okinawa was investigated. The extract of Nandina domestica showed potent activity among these. The inhibitor in the extract was purified by assay-guided fractionation to give a simple phenol glucoside. Although it was a known compound (4-β-D-glucopyranosyloxybenzoic acid), its inhibitory activity toward tyrosinase is revealed for the first time in this work.

著者

Kazushige Goto Naokata Ishii Kaoru Takamatsu

出版者

Japan Society of Physical Education, Health and Sport Sciences

雑誌

International Journal of Sport and Health Science (ISSN:13481509)

巻号頁・発行日

vol.2, pp.111-118, 2004 (Released:2008-01-23)

参考文献数

25

被引用文献数

3 4 6

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We had previously shown that performing a single set of resistance exercise at 50% of 1 repetition maximum (RM) added after a high-intensity, low-repetition exercise (strength-type regimen) greatly enhanced growth hormone (GH) secretion [Goto et al. (2003)]. The present study aimed to investigate the effect of an additional set at 50% to 20% of 1RM after a strength-type regimen on anabolic hormone secretion. Eight male subjects performed bilateral knee extension exercises using a strength-type regimen (5 sets at 90% of 1RM, with 3-min rests), and other 3 types of regimens, in which 1 set of exercise at either 50%, 30% or 20% of 1RM was added immediately after the strength-type regimen (defined as C50-type, C30-type and C20-type regimens, respectively). Concentrations of serum GH, testosterone and blood lactate were measured before and after exercises. The number of repetitions in the added set showed a significant dependence on the exercise intensity: 82.3 times in C20-type > 46.1 times in C30-type > 22.6 times in C50-type (p ≤ 0.05). Post-exercise GH concentrations were significantly (p ≤ 0.05) higher in C50- and C30-type regimens than in the strength-type regimen, whereas no significant difference was observed between C20- and strength-type regimens. Testosterone did not change in any types of regimen. These results indicated that performing a single set of exercise at low intensity added after a strength-type regimen caused a significant increase in GH concentration. However, such an effect might be diminished when the intensity of the additional exercise was extremely low (below 20% of 1RM).

著者

澤 芳樹

出版者

日本医科大学医学会

雑誌

日本医科大学医学会雑誌 (ISSN:13498975)

巻号頁・発行日

vol.5, no.1, pp.22-26, 2009 (Released:2009-04-15)

参考文献数

7

著者

広井 良典

出版者

日本緑化工学会

雑誌

日本緑化工学会誌 (ISSN:09167439)

巻号頁・発行日

vol.35, no.2, pp.283-285, 2009 (Released:2010-07-27)

参考文献数

6

著者

Sung Min NAM Sun Shin YI Dae Young YOO Woosuk KIM Jung Hoon CHOI In Koo HWANG Je Kyung SEONG Yeo Sung YOON

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1203110792, (Released:2012-03-21)

被引用文献数

3 4

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In this study, we investigated diabetic stage dependent cyclooxygenase-2 (COX-2) immunoreactivity in the dentate gyrus in streptozotocin (STZ)-induced type 1 diabetic rats. The animals were sacrificed at 2, 3 and 4 weeks after STZ treatment. Blood glucose levels were increased after STZ treatment. COX-2 immunoreactivity in dentate gyrus was significantly increased in these regions 3 weeks after STZ treatment and restored to its basal level to 4 weeks after STZ treatment. In contrast, COX-2 immunoreactivity was not changed in CA3 region in all groups. These results suggest that STZ-induced type 1 diabetes transiently, but not permanently, decreased synaptic transmission and plasticity 3 weeks after STZ treatment in the dentate gyrus.

著者

荻野 美恵子

出版者

日本神経学会

雑誌

臨床神経学 (ISSN:0009918X)

巻号頁・発行日

vol.48, no.11, pp.973-975, 2008 (Released:2009-01-15)

参考文献数

8

被引用文献数

12 15

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Because the whole of the management of ALS is palliative care, in this paper I presented about the management of each symptom, respiratory care, decision making of the mechanical ventilation, and the end of life care in Japan. We must be aware that the patients with ALS can continue to live if they decide to wear the tracheostomy ventilation (TV) even just before death, it is completely different form situation in cancer patients. In Japan about 20% of ALS patients choose TV, this figure is much higher than western countries. On the other hand, only 14% of neurologist have experience of opioids usage for ALS in 2007, much lower than western countries. We started to use opioids for ALS patients in 2005. We use morphine 10-30mg/day as maintenance dose in early phase without sever side effect. Eighty-eight percent patients reported relief of breathlessness, in 4 patients out of 9, PCO2 was decreased. It is big problem that the health insurance does not cover the cost of opioids for ALS. I want to emphasize that the best effort should be taken to relieve suffering not only for cancer patient but for every patient.

著者

Nao TSUZUKI Jong-Pil SEO Kazutaka YAMADA Shingo HANEDA Yasuhiko TABATA Naoki SASAKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1203160793, (Released:2012-03-22)

被引用文献数

5 8

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Platelet-rich plasma (PRP) was administered to cattle with hoof defects (10-mm drill hole) to promote sole defect healing. Two types of gels were prepared. One was a mixed gel [compound of gelatin microspheres (GM) incorporated with PRP and alginate] and was used for the test group, while the other was an alginate gel used for the control group. Each hole was filled with one of these gels. The drill hole depth, pressure pain, and hardness of the regenerated tissue were measured for 3 weeks after treatment. These measurements improved significantly in the test group than in the control group. The results suggest that a compound of GM incorporated with PRP and alginate had a hoof-regenerating effect in cattle.

著者

横正 健剛 山地 直樹 馬 建鋒

出版者

日本植物生理学会

雑誌

日本植物生理学会年会およびシンポジウム 講演要旨集 第52回日本植物生理学会年会要旨集

巻号頁・発行日

pp.0960, 2011 (Released:2011-12-02)

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ソバはアルミニウム(Al)耐性が強いだけではなく、地上部に高濃度のAlを集積する。生理学的な解析から、ソバはAlに応答し根からシュウ酸を分泌し、地上部ではAlをシュウ酸との錯体で液胞に隔離することが明らかとなっている。これらの過程には多くのトランスポーターの関与が考えられるが未だ同定されていない。私たちはソバのAl耐性及び集積の分子機構を網羅的に解析するために、Alで処理したソバの根からRNAを抽出し次世代シークエンサーを用いてEST解析を行った。均一化したcDNAライブラリーから遺伝子配列の決定を行い約10万のコンティグを作成した。また、均一化していないcDNAライブラリーを用いたショートリード解析から発現量の測定を行った。本研究では、トランスポーター候補遺伝子の中で発現上位にあるFeIREG2についてその全長の単離と機能解析を行った。RACE法を用いて完全長cDNAを単離したところ、FeIREG2はシロイヌナズナのFeとCo、Niの排出トランスポーターAtIREG2とアミノ酸レベルで約60%の相同性を示した。FeIREG2は根で発現し、Al処理6時間後には約20倍に増加していた。この遺伝子とGFPの融合遺伝子をタマネギ表皮細胞に発現させたところ、液胞膜に局在していた。これらのことからFeIREG2はAlの液胞への隔離に関与している可能性が考えられ、更なる解析を進めている。

著者

新岡 祥平 香取 拓 坂田 洋一 林 隆久 井内 聖 小林 正智 太治 輝昭

出版者

日本植物生理学会

雑誌

日本植物生理学会年会およびシンポジウム 講演要旨集 第52回日本植物生理学会年会要旨集

巻号頁・発行日

pp.0438, 2011 (Released:2011-12-02)

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セシウム(Cs)はカリウム(K)と化学的に類似しており、同様の経路を通り植物体に吸収され、微量で植物体に毒性を示す事が知られている。モデル植物Arabidopsis thalianaは世界中で1000種以上のaccessions(エコタイプ)が存在する。accessions間では百塩基に一塩基しか違いがないにも関わらず、形態形成やストレス耐性等に大きなバリエーションがあることが知られている。そこで本研究では、354種のaccessionsを用いてCs耐性評価を行った。その結果、実験系統Col-0と比較して顕著な耐性を示すCs耐性系統と、逆に高感受性を示すCs高感受性系統を見出した。Csストレス下における地上部のCs蓄積量を測定した結果、Cs耐性系統のCvi-0がCsを低蓄積している事が明らかとなった。

著者

Satoru Tsukada Ryota Sakamoto Hiroshi Nishihara

出版者

The Chemical Society of Japan

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

vol.41, no.4, pp.357-359, 2012-04-05 (Released:2012-03-17)

参考文献数

43

被引用文献数

4

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[{Cp*Rh(S2C6H4)}2W(CO)2] (Cp*: η5-C5Me5) (1) and [{Cp*Rh(S2C6H4)}(S2C6H4)W(CO)2] (2) were synthesized by the reaction of rhodadithiolene [Cp*Rh(S2C6H4)] with [W(CO)3(py)3], and their crystal structures and physical properties were investigated. The formation mechanism of 2 from 1 was pursued by 1H NMR spectroscopy. 1 reacted with an excess amount of BF3·Et2O to furnish 2, indicating that a Lewis acid assisted the demetalation reaction.

著者

Seung Bin CHA An Na YOO Won Jung LEE Min Kyoung SHIN Myung Hwan JUNG Seung Won SHIN Young Wook CHO Han Sang YOO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1203110791, (Released:2012-03-21)

被引用文献数

7 28

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We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage CJ12 in ETEC infected pigs. Phage was mixed with feed at a ratio of 1:1,000 (0.1%). One week after initially providing phage mixed feed, pigs were challenged orally with 1011 CFU of ETEC and body weight, diarrhea score, bacterial CFU and phage PFU in the feces were measured. Pigs of phage treated groups C (106 PFU/g) and D (108 PFU/g) showed more resistance to diarrhea due to ETEC infection compared to positive control group B on the third day after the initial challenge. Moreover, during the quantitation of ETEC in feces, both groups C and D showed approximately 63.92 and 60.73% reduced ETEC compared to positive control group B. Phages were successfully isolated from feces in both groups C and D during the experiment without any adverse effects, suggesting the possibility of using CJ12 as a feed additive.

著者

伊藤 俊彦

出版者

日本鉱物科学会

雑誌

岩鉱 (ISSN:09149783)

巻号頁・発行日

vol.91, no.6, pp.209-219, 1996 (Released:2006-11-25)

参考文献数

22

被引用文献数

8 27

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氷点下の気温になる冬季間,北海道足寄町螺湾のシオワッカ冷泉からの弱い流れの中で,ikaiteが生じている。積雪の少ない年には,氷に閉じこめられた産状でikaiteからなる輪縁石ダムが見られた。海洋,湖沼以外である流水域におけるikaiteの生成はこれまでは知られていない。先年の夏に同地域から,calciteといっしょにmonohydrocalciteの生成が知られたところから,冬のikaiteの生成が予想された。シオワッカ冷泉石灰華からはikaiteからの転移鉱物のvateriteを加えると,aragoniteを除き,自然界での炭酸カルシウム塩鉱物のほとんど全てが得られた。本地における夏から冬の間の炭酸カルシウム塩鉱物の生成の過程を,産状から考察した。これらの鉱物の生成における必要条件は,水質のほか,水温(ikaite,calcite),地表面の濡れ(monohydrocalcite:伊藤,1993),など,それぞれによって異なる。また,シオワッカ冷泉の水質,ikaiteおよびvateriteのX線粉末回折線値,ikaiteから転移した後の方解石の化学組成を示した。     Ikaiteが冷たい海域,深海堆積物から次々と発見されたことから,1982年来,これまで源鉱物の明らかでなかったglendoniteや玄能石と呼ばれてきた方解石仮像の源鉱物がikaiteであるとする説が多く出されてきた。しかし,ikaiteの結晶形と一般的な大きさはこれまでに知られている方解石仮像と一致しない。ここでは,方解石仮像に特徴的な四角錐に対応する結晶形が,走査型電子顕微鏡で観察されたikaiteの結晶面の組み合わせによって導かれることを示した。Ashoro-cho 足寄町,Rawan 螺湾,Shiowakka シオワッカ,Konbumori 昆布森

著者

Keisuke Tomono Hajime Katsuyama Akira Tomono

出版者

一般社団法人 映像情報メディア学会

雑誌

映像情報メディア学会誌 (ISSN:13426907)

巻号頁・発行日

vol.65, no.10, pp.1411-1419, 2011-10-01 (Released:2011-12-20)

参考文献数

12

被引用文献数

1 1

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We propose a new method in which scents are emitted through the display screen in the direction of the viewer to enhance the reality effect of the visual images. A thin LED display panel filled with tiny pores was made for this experiment and an air control system with a blower was placed behind the screen. We experimentally proved that the direction of airflow could be controlled and scents could properly travel through the pores to the front side of the screen aimed at the viewer. The effectiveness of the scents and the psychological effects were evaluated by the subjects' biological responses and answers to a questionnaire. By analyzing their biological responses, more significant changes in skin conductance were detected when the advertisements were presented with visuals and scents than with visuals alone. Therefore, we had a lot of good reasons to objectively evaluate the psychological effects of visuals with scents.

著者

向坂 真一 寺本 華奈江 小池 英樹

出版者

一般社団法人 日本質量分析学会

雑誌

Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)

巻号頁・発行日

pp.1202230021, (Released:2012-02-27)

参考文献数

18

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for analysis of not only biological materials but also synthetic polymers. A mass spectrum obtained by MALDI-MS was found to have peaks at equal intervals on the m/z axis. The chemical structure of many types of biological compounds contain repeating units that have exactly the same elemental composition. If these repeated peaks could be identified based on the m/z interval value, such analyses could be optimized even further from the point of view of chemical structure. In this study, we describe a system for visualizing such data that has the capability of analyzing the repeated peaks on the m/z axis. The system, mzRepeat, was developed and evaluated by applying the mass spectrum of polyethylene glycol and mycolic acid, which was obtained using a JMS-S3000 Spiral-TOFMS system. Because mzRepeat can systematically show a mass spectrum that includes different numbers of repeating units, it has the potential for use in anlalyzing these types of molecules.

著者

浜田 宏

出版者

数理社会学会

雑誌

理論と方法 (ISSN:09131442)

巻号頁・発行日

vol.24, no.1, pp.57-75, 2009-05-25 (Released:2010-01-08)

参考文献数

15

被引用文献数

4

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教育達成の不平等を説明するBreen and Goldthorpeの相対リスク回避モデルを一般化して、上層出身者の進学率が中層出身者の進学率を上回る条件を示す。上・中層出身者の進学率をパラメータの明示的な関数として示すことで、教育達成格差をオッズ比として表し、解析的に分析する。また高校進学後に大学進学の分岐が続く場合のように、学歴の推移が連続して生じる状況をモデル化して、理論的な進学率を導出する方法を定式化する。拡張した数理モデルを用いて、教育水準が高くなるほど出身階層の影響が弱くなる階層効果逓減現象が、どのような条件で生じるのかを示す。オッズ比の低下は中層出身者が上層到達を選好する場合は高等教育段階での進学率が上層出身者の進学率に追いつくことによってもたらされる。無差別のときは中層進学率が定数になる一方で、教育水準の上昇により進学後の主観的成功確率の分布がマイナス方向にシフトして、上層出身者のみ進学率が減少することによってオッズ比が減少する、という予想が得られた。

著者

Sayumi SHIMODE Takayuki MIYAZAWA Takeshi KOBAYASHI Hisaaki SATO Taishi TANABE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.2, pp.235-239, 2012 (Released:2012-03-02)

参考文献数

21

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Interferon-stimulated gene 15 (ISG15) is one of the type I interferon-inducible proteins. Addition of ISG15 known as ISGylation is an ubiquitin-like posttranslational modification. Coexpression of ISG15 and ubiquitin-activating enzyme E1-like protein (UBE1L) is required to induce ISGylation. Previously, we identified feline ISG15 gene and found that the capsid protein of feline immunodeficiency virus was ISGylated in vitro by treatment with feline interferon-ω. In this study, we cloned feline UBE1L (FeUBE1L) gene to further study the mechanism of the antiviral activities induced by ISGylation. Sequencing analysis revealed that active sites of FeUBE1L were highly conserved. These data suggest that FeUBE1L has an enzymatic activity. Further, expression of FeUBE1L was induced in feline cell lines by treatment with feline interferon-ω and ovine interferon-τ.

著者

Eiichi KAWAKAMI Tomoko YAGI Masanori KOBAYASHI Tatsuya HORI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.2, pp.201-204, 2012 (Released:2012-03-02)

参考文献数

14

被引用文献数

2 2

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A Beagle dog (3 years old) that ejaculated high percentages (mean ± SE: 29.1 ± 1.2%) of sperm with a knobbed acrosome abnormality and a low number of sperm and that also had a low plasma testosterone (T) level was given 10 subcutaneous injections of 1 μg of gonadotropin-releasing hormone analogue (GnRH-A) at 3-day intervals. The plasma T level and number of sperm increased 12-14 weeks after the first injection. Although the percentages of sperm with knobbed acrosome abnormality did not change after the GnRH-A therapy, the number of sperm and percentage of actively motile sperm increased after the therapy, and a bitch gave birth to 5 healthy puppies after intravaginal artificial insemination with fresh semen collected 14 weeks after the first injection.