著者

片峰 茂 堂浦 克美 金子 清俊 小野寺 節 福岡 伸一 堀内 基広

出版者

長崎大学

雑誌

基盤研究(C)

巻号頁・発行日

2002

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本邦におけるプリオン研究者の情報交換の促進と将来の共同研究プロジェクト立ち上げのための準備を目的に本研究を遂行した。情報交換に関しては、平成14年10月21日に長崎において班会議を開催し、班員に加えて数名の内外のプリオン研究者による情報交換と討議の場をもった。その結果、個々の班員間の往来及び研究材料の共有などのいくつかの実が挙がっている。例えば、片峰と小野寺は各々が開発したプリオン蛋白遺伝子に関わる遺伝子改変マウスと培養細胞株を共有することにより、プリオン病神経変性の機構解明へ向けた共同研究の進展が図られた。準備研究に関しては、プリオン研究進展に極めて大きな意味をもつ種々のモデル動物、細胞株、抗体、解析システムの開発が行われ、将来の大型共同研究プロジェクトへの準備は整ったと考えられる。以下に特筆される成果を挙げる。(1)プリオン持続感染細胞株の樹立(片峰)(2)プリオン類似蛋白(Dpl)遺伝子トランスジェニックマウスの樹立(片峰)(3)プリオン蛋白(PrP)と相互作用をする分子の同定法の開発(堂浦)(4)異常プリオン蛋白(PrPSc)に特異的立体構造を認識する抗体の確立(堀内)(5)不死化によるPrP欠損神経細胞株の樹立(小野寺)(6)PrPの細胞内挙動の顕微鏡下での追跡法の確立(金子)(7)タンパク質の2次構造変換定理の発見(柳川)(8)微量核酸(RNA)同定法の開発(福岡)本年度は、他領域との重複などの問題点があり、新規特定領域への申請は見送ったが、本研究の成果を基礎に来年度以降の申請へむけ、さらなる体制整備を行う予定である。

著者

菅田 節朗 山之内 正子 松島 美一

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.25, no.5, pp.884-889, 1977-05-25 (Released:2008-03-31)

被引用文献数

20 30

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Three isomers of meso-tetrapyridylporphins, i. e. tetra (2-pyridyl)-(2), (3-pyridyl)-(3), and (4-pyridyl)-(4), porphin have been synthesized. They were soluble in acetic acid, chloroform and acidic aqueous solvents. Solubilities in chloroform, dimethylformamide and pyridine were in the order 3>2>4. Comparison of the visible spectra indicated that the intensities of the bands due to 0-0 transitions decreased (3>4>2) with an increase of the electron-withdrawing character of the pyridyl substituent. The copper (II) and zinc (II) complexes of 2 and 3 have been prepared. Infrared and nuclear magnetic resonance spectra and their assignments of the porphins and/or the metal complexes were described.

著者

白川 充 山口 忠敏 深町 征二郎 菅田 節朗

出版者

一般社団法人 日本農村医学会

雑誌

日本農村医学会雑誌 (ISSN:04682513)

巻号頁・発行日

vol.28, no.5, pp.660-674, 1980-01-30 (Released:2011-08-11)

参考文献数

13

著者

河野 彬 菅田 節朗 原 泰寛 田中 睦子 加留部 喜晴 松島 美一

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.31, no.5, pp.1666-1669, 1983-05-25 (Released:2008-03-31)

参考文献数

19

被引用文献数

2

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A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5'-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0, and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

著者

河野 彬 原 泰寛 菅田 節朗 松島 美一 上田 亨

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.32, no.5, pp.1919-1921, 1984-05-25 (Released:2008-03-31)

参考文献数

15

被引用文献数

12 16

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A thymidine phosphorylase preparation was partially purified from human liver tumor tissues (poorly differentiated adenocarcinoma). The substrate specificity of the enzyme was investigated with eleven pyrimidine nucleosides. Thymidine and 2'-deoxyuridine were good substrates, while uridine, 3'-deoxyuridine, 5'-deoxyuridine, and 2', 3'-dideoxy-3'-hydroxy-methyluridine were not. Uridines substituted at the 5-position by a cyano, bromo, or chloro group were also phosphorolyzed by the enzyme, but the activity for 5-fluorouridine was much lower. 5'-Deoxy-5-fluorouridine was also cleaved. Either a 5-substituent or a 2'-deoxy structure seems to be essential for a good substrate.

著者

河野 彬 原 泰寛 菅田 節朗 加留部 善晴 松島 美一 石塚 秀夫

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.31, no.1, pp.175-178, 1983-01-25 (Released:2008-03-31)

参考文献数

20

被引用文献数

77 93

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Activities of pyrimidine nucleoside phosphorylases were assayed in extracts of human tumors, normal tissues of the same organs and tumors of mice (Sarcoma-180) and guinea pigs (Line-10), with thymidine (dThd), uridine (Urd), and 5'-deoxy-5-fluorouridine (5'-DFUR) as substrates. The nucleoside cleaving activities were higher in extracts of human tumor tissues than in those of normal tissues of the same organs. In human tissues, phosphorolytic activitiy towards dThd was high, while that towards Urd was low. In animal tumors, Urd was the best substrate. 1-(2'-Deoxy-β-D-glucopyranosyl)-thymine (GPT), a specific inhibitor of uridine phosphorylase, inhibited the phosphorolysis of Urd and 5'-DFUR in extracts of animal tumors, but not that of dThd and 5'-DFUR in extracts of human tumors. A thymidine phosphorylase preparation was partialy purified from human lung cancer. Km values of the preparation were 2.43×10-4 M and 1.69×10-3 M for dThd and 5'-DFUR, respectively. We conclude that in human tumors a thymidine phosphorylase activity converts 5'-DFUR to 5-fluorouracil, an activated form.

著者

菅田 節郎 河野 彬 原 泰寛 加留部 善晴 松島 美一

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.34, no.3, pp.1219-1222, 1986-03-25 (Released:2008-03-31)

参考文献数

16

被引用文献数

5 11

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A thymidine phosphorylase (TP) preparation was partially purified from human gastric cancer (poorly differentiated adenocarcinoma). The specific activity of the final preparation represented a 379-fold purification of the 7000g supernatant of tissue homogenate. The phosphorolytic activities toward thymidine (dThd), 5'-deoxy-5-fluorouridine (5'-DFUR), and 1-(tetrahydro-2-furanyl)-5-fluorouracil (Tegafur) remained closely in parallel during the whole purification procedure. The results provide evidence in support of the assumption that 5'-DFUR and Tegafur are converted into 5-fluorouracil, an activated form of the antitumor agents, in humn tumor tissues by a TP activity. The values of Km of the TP preparation were 1.68×10-4, 1.72×10-3, 1.33×10-2, and 4.76×10-2M for dThd, 5'-DFUR, Tegafur, and uridine, respectively.

著者

菅田 節朗

出版者

九州大学

巻号頁・発行日

1978

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博士論文

著者

松田 芳郎 周防 節雄

出版者

日本統計協会

雑誌

統計 (ISSN:02857677)

巻号頁・発行日

vol.49, no.12, pp.13-18, 1998-12

著者

高橋節夫 編

出版者

小島忠国

巻号頁・発行日

1891

著者

内田すえの 此川純子 堀江節子著

出版者

桂書房

巻号頁・発行日

1992

著者

川村 英樹 山元 拓哉 長友 淑美 鶴 亜里沙 石堂 康弘 横内 雅博 井尻 幸成 小宮 節郎

出版者

西日本整形・災害外科学会

雑誌

整形外科と災害外科 (ISSN:00371033)

巻号頁・発行日

vol.56, no.2, pp.277-281, 2007 (Released:2007-06-01)

参考文献数

5

被引用文献数

1

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25歳女性.妊娠34週突然左腰殿部痛出現.仙骨硬膜外ブロック施行されるも疼痛軽減せず当院産科に緊急搬送となる.入院時疼痛強く仰臥位不能,左仙腸関節部に圧痛を認めた.発熱,炎症所見(白血球数9400/μl,CRP 11.91)があり,骨盤部MRI上左仙腸関節周囲の信号変化,また血液培養,カテーテル尿培養よりメチシリン感受性黄色ブドウ球菌を認め,尿路感染に伴う菌血症により発生した化膿性仙腸関節炎と診断し,セファゾリンによる抗菌化学療法を開始.症状改善し妊娠39週にて帝王切開により出産.母子ともに経過良好であり産後3週自宅退院となる.仙腸関節は菌血症性関節炎の好発部位の一つであり,妊娠にともなう子宮の増大等が誘因と考えられた.胎児への影響を考慮する必要があるため診断,治療に難渋するが,妊婦の腰殿部痛の原因として化膿性仙腸関節炎も鑑別する必要がある.

著者

馬場 駿吉 高坂 知節 馬場 廣太郎 斎藤 洋三 坂倉 康夫 岩田 重信 西村 忠郎 石川 哮 小川 暢也

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.12, no.4, pp.379-397, 1992-07-10 (Released:2010-04-12)

参考文献数

11

被引用文献数

4 2

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The therapeutic effects of IPD-1151 T, a new anti-allergic agent, were compared with those of Tranilast in 356 patients with allergic rhinitis. The daily dosage was 300 mg (one capsule each, t.i.d.) for IPD-1151 T and 300 mg (one capsule each, t.i.d.) for Tranilast. Both test drugs were orally given for 6 weeks.The results of this study are summarized as follows :(1) IPD-1151 T showed significant superiority to Tranilast in “final global improvement rating”. Improvement rating was 55.0% for IPD-1151 T and 37.8% for Tranilast in terms of “moderate” plus “marked” improvement category.(2) The item evaluated, “overall safety rating” showed significant superiority of IPD-1151 T to Tranilast. The incidence of an adverse reaction or abnormal laboratory finding was noted as 3.7% fo IPD-1151 T and 11.0% of Tranilast.(3) The item evaluated, “global utility rating” showed significant superiority of IPD-1151 T to Tranilast. The utility rating was calculated as 53.2% of IPD-1151 T and 34.7% of Tranilast in terms of “moderately” plus “markedly” useful category.(4) Side effects were observed in 6 patients (3.7%) of IPD-1151 T and 18 patients (11.0%) Tranilast. All of these symptoms disappeared by symptomatic treatment or discontinued test drug.All the above results indicate that IPD-1151 T is safer and more effective than Tranilast. Therefore, IPD-1151 T is judged as a highly useful anti-allergic agent for the treatment of allergic rhinitis.

著者

仲 立貴 中島 節子 韓 一栄 今野 廣隆 呉 泰雄

出版者

日本体育大学

雑誌

日本体育大学紀要 (ISSN:02850613)

巻号頁・発行日

vol.39, no.2, pp.93-99, 2010-03-30

著者

森 謙作 寺田 雅之 石井 一彦 本郷節之

出版者

一般社団法人情報処理学会

雑誌

情報処理学会研究報告コンピュータセキュリティ(CSEC) (ISSN:09196072)

巻号頁・発行日

vol.2004, no.129, pp.11-16, 2004-12-20

参考文献数

9

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電子価値を公平に交換可能なプロトコルをICカードへ実装し,プロトコルの実用性の検証を行った.実装にあたり,電子財布などの取引アプリケーションからの利用が容易であること,および電子価値の交換を電子商取引として実用的な速度で行えることを目的とした.また,ICカードのI/F設計において分散ICカード環境のためのフレームワークであるTENeTを利用することにより,ICカード間のメッセージのやり取りをアプリケーションから隠蔽し,ICカードとアプリケーションとのI/Fを簡素化することができた.実装の結果,プロトコルのコードサイズは約6KB,プロトコル処理時間は約1.8secであり,現在市販されているICカードを用いて,上記プロトコルを実用的な性能で実現可能であることを示した.This paper reports the result of our implementation of the fair exchange protocol for electronic rights on smartcards. In this implementation, we adopted TENeT, a framework which enables smartcards to directly communicate one another, to simplify the interface between smartcard and application programs. Our implementation built on current smartcards can exchange electronic rights in less than 1.8sec, which is practically sufficient for electronic rights trading markets.

著者

藤井 節郎 奥田 拓道 赤沢 明 安田 行寛 川口 安郎 福永 育史 西川 栄郎

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.95, no.6, pp.732-740, 1975-06-25 (Released:2008-05-30)

参考文献数

13

被引用文献数

4 5

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In order to investigate the fate of 1-(2-tetrahydrofuryl)-5-fluorouracil (FT-207) in comparison with that of 5-fluorouracil tritiated FT-207 (3H-FT-207) and 5-fluovouracil (3H-5-fluorouracil)were administered from the rectum in normal and AH-130 tumor-bearing rats, and rapid absorption of 3H-FT-207 or 3H-5-fluorouracil, was observed. Blood level of radioactivity after rectal administration of 3H-FT-207 was higher and more continuous than that of 3H-5-fluorouracil. Although the radioactivity after rectal administration of 3H-FT-207 and 3H-5-fluovouracil was widely distributed in the various tissues of the animal with or without tumor, the highest concentration of the radioactivity was observed in the kidneys, and higher in tumor. The radioactivity in lymphatic gland reached a maximum level within 2-4 hr after the rectal administration of 3H-FT-207 and declined very slowly. The urinary excretion of radioactivity within 24 hr was about 30% of the administered dose of 3H-FT-207. More than 85% of the excreted radioactivity accounted for FT-207 and its metabolite, α-fluoro-β-alanine. Other metabolites such as 5-fluorouracil, α-fluoro-β-ureidopropionic acid and tritiated water were detected in small amounts in rat urine. The radioactivity in blood and tumor, 4 hr after rectal administration of 3H-FT-207, was found to represent FT-207. However, the radioactivity in liver was due to the presence of α-fluoro-β-alanine. About 90% radioactivity in lymphatic gland within 2-6 hr after rectal administration of 3H-FT-207 was detected as FT-207.

著者

増島宏 高橋彦博 大野節子著

出版者

法政大学出版局

巻号頁・発行日

1969

著者

[ヴィクトル・ユゴー著] 西節夫訳 . [ヴィクトル・ユゴー著] 杉山正樹訳

出版者

潮出版社

巻号頁・発行日

2001

著者

中村 節子 (旧姓森中

出版者

一般社団法人 日本めまい平衡医学会

雑誌

Equilibrium Research (ISSN:03855716)

巻号頁・発行日

vol.72, no.3, pp.145-155, 2013 (Released:2013-08-01)

参考文献数

52

被引用文献数

1

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There have been several reports about the relation between acid-base imbalance or arterial blood gas abnormalities and the occurrence of vertigo, including Meniere' disease. In animal experiments, respiratory acidosis induced by CO2 inhalation or metabolic acidosis induced by injection of NH4Cl has been shown to cause attacks of vertigo in rabbits with hemilabyrinthectomy. In clinical studies, approximately half of the patients with dizziness have arterial blood gas abnormalities when their dizziness occurs. An increase of HCO3- is found in many patients with dizziness, and the frequency of attacks is higher in patients who have arterial blood gas abnormalities during the remission period. These patients are thought to have unilateral vestibular dysfunction, and it is suggested that arterial blood gas abnormalities cause temporary vestibular dehabituation that increases the frequency of dizziness. It has also been reported from a study that the middle ear pressure difference between both ears, which is larger during periods of dizziness or recurrent dizziness than at the time of remission, might be related to blood gas abnormalities in Meniere's disease. In the same study, a difference of more than 50 decapascals was significantly more common in the patient group with blood gas abnormalities. In addition, metabolic acidosis has been reported in patients with Meniere's disease, and the base excess and bicarbonate levels were also found to be beyond the normal range in Meniere's disease. Arterial blood gas abnormalities might develop for various reasons, including underlying diseases and middle ear pressure differences. The endolymph in the endolymphatic sac is acidic (pH6.6-7). It has been reported that carbonic anhydrase, vacuolar H+-ATPase, pendrin and aquaporin may participate in the acidification process or homeostasis. The role of acid-base disturbance or arterial blood gas abnormalities in dizziness, and the relation in acid-base balance between inner ear and arterial blood gas need to be investigated further.

著者

川口 安郎 中村 芳正 佐藤 俊幸 武田 節夫 丸中 照義 藤井 節郎

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.98, no.4, pp.525-536, 1978-04-25 (Released:2008-05-30)

参考文献数

14

被引用文献数

5 8

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After oral administration of 5-fluoro-1, 3-bis (tetrahydro-2-furanyl)-2, 4-pyrimidinedione (FD-1), the level of 5-fluoro-2, 4-pyrimidinedione (5-FU) was 5 to 7 times higher in the plasma and normal tissues and 8 to 12 times in tumor tissue than after administration of 5-fluoro-1-(tetrahydro-2-furanyl)-2, 4-pyrimidinedione (FT). Moreover, these levels were maintained longer than after administration of FT. In tumor tissue, the concentration of 5-FU was still as high as 1.42 μg/g 12 hr after administration of FD-1. FD-1 was degraded to 5-fluoro-3-(tetrahydro-2-furanyl)-2, 4-pyrimidinedione (3-FT) by liver microsomal drug-metabolizing enzymes in vitro and to FT spontaneously. Subsequently, FT was converted enzymically to the active substance, 5-FU, and 3-FT changed to 5-FU spontaneously. Conversion of FD-1 to 5-FU via 3-FT was greater than via FT. It is concluded that a large amount of 5-FU formed after administration of FD-1 is formed via 3-FT. γ-Hydroxybutyric acid was found to be formed in vivo and in vitro from the tetrahydrofuranyl group of FD-1.