著者

江口 勝久

出版者

佐賀県玄海水産振興センター

巻号頁・発行日

no.7, pp.81-97, 2015 (Released:2016-01-20)

著者

中島 博司

出版者

三重県水産研究所

巻号頁・発行日

no.17, pp.61-64, 2009 (Released:2011-07-19)

著者

山浦 啓治 江口 勝久

出版者

佐賀県玄海水産振興センター

巻号頁・発行日

no.7, pp.1-4, 2015 (Released:2016-01-20)

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付着珪藻を餌料とした稚ナマコ飼育時における適正照度を明らかにするため,照度(遮光率)の異なる3~4区の飼育試験区を設定し,各試験区での稚ナマコの成長と生残を比較した。その結果,アカナマコ,アオナマコの飼育試験に共通して,飼育時の照度が高いほど(遮光率が低いほど)生残,成長共に良くなるという結果が得られた。遮光率別の各試験区の付着珪藻の種類,細胞密度,被覆度を調べた結果,稚ナマコの餌料として適する種類の量は照度が高いほど多く,このことが飼育結果に反映していると考えられた。

著者

藤崎 博 岡山 英史 青戸 泉

出版者

佐賀県玄海水産振興センター

巻号頁・発行日

no.3, pp.39-42, 2005 (Released:2012-12-06)

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アオナマコの稚ナマコの生残と照度との関係を調べるため、照度500luxl~10,000luxの範囲で、体長0.5mm、2mm、4mmのサイズ別に、10日間試験し、生残率を比較した。1. 10日間の稚ナマコ飼育結果で70%以上の高生残率を示した照度は、体長0.5mmサイズで500lux及び1,000lux区、体長2mmサイズで500lux、1,000lux及び3,000lux区、体長4mmサイズで1,000lux、3,000lux、10,OOOlux(無繁殖)区であった。2. アオナマコの稚ナマコ飼育に好適な照度は、付着珪藻の存在下でほぼ止水に近い状態では、2mmサイズまでは1,OOOlux以下、4mmサイズでは3,OOOlux以下と考えられた。

著者

浜田 文夫 河本 良彦

出版者

山口県内海水産試験場

巻号頁・発行日

no.15, pp.14-25, 1987 (Released:2011-03-05)

著者

野口 浩介 野田 進治

出版者

佐賀県玄海水産振興センター

巻号頁・発行日

no.6, pp.15-20, 2013 (Released:2014-02-07)

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マナマコ種苗生産では,付着珪藻を繁茂させた付着珪藻板に採苗する生産方式飼育を行っているが,この付着珪藻板上にコペポーダが大量に増殖する問題があるため,炭酸ガス通気海水を用いた除去法の開発を行った。まず,コペポーダ,稚ナマコの活動に与える影響を検討し,次に量産規模でのコペポーダ除去法の検討を行った。pH5.2以下の炭酸ガス通気海水に30分間浸漬することで,コペポーダを除去できたが,2時間浸漬すると稚ナマコも斃死することが判明した。また,量産規模でもコペポーダを除去することができたが,除去後約10日間で再びコペポーダが増殖し,注水海水から侵入することが判明した。そこで,注水口に10μmフィルターを取り付けることで,コペポーダの増殖を約25日間遅延することができた。

著者

野口 浩介 野田 進治

出版者

水産総合研究センター

巻号頁・発行日

vol.3, no.2, pp.131-135, 2011 (Released:2012-12-06)

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ナマコ種苗生産で発生するコペポーダの防除法確立のための基礎試験を行った。種苗生産現場で発生するコペポーダはシオダマリミジンコと判定され,0.2個体/cm2の密度では稚ナマコの初期餌料となる付着珪藻は維持できるが,2.0個体/cm2では約7日間で全て摂餌され,体長0.4~1.0mmの稚ナマコはコペポーダ密度0.2個体/cm2以上で斃死することがわかった。付着珪藻を繁茂させたシャーレに稚ナマコとコペポーダを混在させた場合,コペポーダはまず付着珪藻を摂餌し,付着珪藻が減少するまでの期間,稚ナマコが斃死しないことが判明した。また,UV発光するFITC染色した稚ナマコを斃死させたコペポーダを蛍光顕微鏡で観察したところ,脚部付近は強く発光するが,胃や糞では発光せず,この結果から稚ナマコの斃死要因は食害ではなく,接触ダメージであると推測された。

著者

横山 博 長澤 和也

出版者

広島大学大学院生物圏科学研究科

雑誌

生物圏科学 : 広島大学大学院生物圏科学研究科紀要 (ISSN:13481371)

巻号頁・発行日

vol.53, pp.73-97, 2014

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日本産養殖魚介類の寄生虫の標準和名目録を作成した。国内の養殖対象魚介類に寄生する微胞子虫類7種,鞭毛虫類4種,繊毛虫類7種,粘液胞子虫類32種,単生類24種,吸虫類9種,条虫類3種,線虫類10種,鉤頭虫類6種,ヒル類5種,甲殻類30種を含む合計137種の寄生虫について標準和名を整理し,うち40属,77種について新標準和名あるいは改称を提案した。また,日本における発生事例や寄生虫の生物学や病理学など,魚病学的に重要な参考文献を付記した。

著者

遠藤 守人

雑誌

八戸大学紀要

巻号頁・発行日

no.32, pp.13-18, 2006-03

著者

小林 俊将

出版者

緑書房

雑誌

養殖 (ISSN:00440671)

巻号頁・発行日

vol.47, no.4, pp.86-89, 2010-03

著者

Matsuura Hiroshi Yazaki Ikuko Okino Tatsufumi

出版者

Springer Japan

雑誌

Fisheries Science (ISSN:09199268)

巻号頁・発行日

vol.75, no.3, pp.777-783, 2009-05

被引用文献数

25

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Larval metamorphosis inducers of the sea cucumber Apostichopus japonicus were screened from physiologically active compounds. Doliolaria larvae completed their metamorphosis to juveniles in 120 hours when treated with 5-10 μM of dopamine and L-DOPA, and 50 μM of L-adrenaline and L-noradrenaline. Doliolaria larvae had to be exposed to dopamine or L-DOPA for at least 24 h. D1-like dopamine receptor antagonists SKF87566 and LE300 (10 μM) inhibited metamorphosis by dopamine. However, the D2-like dopamine receptor antagonists sulpiride and nemonapride (10 μM) did not inhibit the effect of dopamine. The results suggest that D1-like dopamine receptors are involved in larval metamorphosis of the sea cucumber A. japonicus.

著者

TANIKAWA EIICHI

出版者

THE FACULTY OF FISHERIES, HOKKAIDO UNIVERSITY

雑誌

MEMOIRS OF THE FACULTY OF FISHERIES HOKKAIDO UNIVERSITY (ISSN:00183466)

巻号頁・発行日

vol.3, no.1, pp.1-91, 1955-08

著者

坂田 由紀子

出版者

日本家政学会

雑誌

日本家政学会誌 (ISSN:09135227)

巻号頁・発行日

vol.41, no.7, pp.p637-645, 1990-07

被引用文献数

2

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(1) The manners of holding chopsticks of 235 female college students in Kyoto have been classified into two types, i. e., S1 of a traditional type and S2 of the other. S1 was 46% and S2 was 54%, and the former was smaller than the number reported by Mukai et al. in 1978. (2) The difference between S1 and S2 was statistically significant when examined in difficult manipulation. (3) The manner of holding chopsticks relates to the behavior in living environment. Members of S1 have been continually trained by their parents since their childhood. Members of S2 feel it very difficult to manipulate chopsticks properly at table.

著者

宮本 孝二

出版者

桃山学院大学

雑誌

桃山学院大学社会学論集 (ISSN:02876647)

巻号頁・発行日

vol.44, no.1, pp.1-23, 2010-10-30

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This paper aims to show analytical effectiveness of social system theory through rethinking 'Social Theory and Social System' which is the range table of possible questions of sociology in Japanese national test of social welfare qualifications. We define social system as patterned relationships among status-role sets to attain common goals, and explain three ways how social systems can be found and analyzed. First, societies are analyzed as general and / or total system from aspects of economy, polity, law, education, social consciousness, stratification, population, social change, and so on. Second, regional society, organization, family, and life are analyzed as individual and / or sectional system. Third, social problematic situations are analyzed as social system responding to and / or causing social problems which are inequality, discrimination, poverty, deviance, suicide, violence, bullying, harassment, environmental problems, and so on.

著者

Lee Chuin Chen

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.2, pp.S0079, 2019-08-26 (Released:2019-08-26)

参考文献数

87

被引用文献数

7

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Increasing the operating temperature of the liquid chromatography (LC) column has the same effect as reducing the diameter of the packing particles on minimizing the contribution of C-term in the van Deemter equation, flattening the curve of plate height vs. linear velocity in the high-speed region, thus allowing a fast LC analysis without the loss of plate count. While the use of smaller particles requires a higher pumping pressure, operating the column at higher temperature reduces the pressure due to lower liquid viscosity. At present, the adoption of high-temperature LC lags behind the ultra-high-pressure LC. Nevertheless, the availability of thermally stable columns has steadily improved and new innovations in this area have continued to emerge. This paper gives a brief review and updates on the recent advances in high-temperature liquid chromatography (HTLC). Recent efforts of hyphenating the capillary HTLC with mass spectrometry via a super-atmospheric pressure electrospray ionization is also reported.

著者

Ami Kageyama (Kaneshima) Akira Motoyama Mitsuo Takayama

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.1, pp.A0077, 2019-11-30 (Released:2019-11-30)

参考文献数

24

被引用文献数

7

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The influence of solvent composition and surface tension on the signal intensity of deprotonated molecules [M−H]− in electrospray ionization mass spectrometry (ESI MS) was evaluated using alanine (Ala), threonine (Thr) and phenylalanine (Phe), which have differing levels of hydrophobicity. The surface tension of the ESI solution was varied by changing the ratio of the organic solvents methanol (MeOH) and acetonitrile (MeCN) in water (H2O). In ESI MS, the signal intensity of all the amino acids was increased with decreasing surface tension for the two solutions, H2O/MeOH and H2O/MeCN. The use of H2O/MeCN was more favorable for achieving a strong signal for the analytes compared to H2O/MeOH. The smaller vaporization enthalpy of MeCN compared to MeOH was proposed as one of the most plausible explanation for this. The order of the signal intensity of amino acids was Phe>Thr>Ala, the same order as their hydrophobicity. It can be practically concluded that the use of solutions with lower surface tensions and lower vaporization enthalpies would result in higher signal intensities in ESI MS.

著者

Hisao Nakata

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.1, pp.A0074, 2019-11-25 (Released:2019-11-25)

参考文献数

46

被引用文献数

4

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Typical modes of bond cleavages of organic compounds in mass spectrometry are briefly summarized. Although these fragmentation rules can be quite useful for interpreting mass spectra of simple compounds, application to structurally complex molecules that contain multiple hetero atoms such as nitrogen or oxygen becomes increasingly difficult, because the exact location of an unpaired electron or positive or negative charges becomes obscure in precursor ions.About a decade ago, we proposed “a rule of mass shift,” which correctly predicts the m/z for observed peaks corresponding to singly charged even-electron fragment ions. The basis of the rule postulates that ions observed as peaks in an ordinary mass spectrum should be sufficiently stable to survive during the flight path in a mass spectrometer.The important recognition is that each atom in a stable ion should be in an ordinary valence state, and no free valence should be allowed. Therefore, if the cleavage of a bond leads to an ion with an unstable structure, some structural changes must take place in order for the ion to be observed in the mass spectrum. Such structural changes can be the addition of hydrogen atom(s) and/or a proton for positive ions, and the addition of a hydrogen atom and/or the elimination of two hydrogen atoms in the case of negative ions. These required structural changes in each case are schematically depicted and discussed in detail.Two typical examples are shown, in which m/z’s of the observed peaks are correctly predicted. The scope and limitations, as well as the significance of the rule for analyzing fragmentations in organic mass spectrometry are also discussed.

著者

Nobuyuki Okahashi Shuichi Kawana Junko Iida Hiroshi Shimizu Fumio Matsuda

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.1, pp.A0073, 2019-08-30 (Released:2019-08-30)

参考文献数

15

被引用文献数

1 18

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Isotope labeling measurements using mass spectrometry can provide informative insights on the metabolic systems of various organisms. The detailed identification of carbon positions included in the fragment ions of dicarboxylic and tricarboxylic acids in central carbon metabolism is needed for precise interpretation of the metabolic states. In this study, fragment ions containing the carbon backbone cleavage of dicarboxylic and tricarboxylic in the Krebs cycle were investigated by using gas chromatography (GC)-electron ionization (EI)-MS and GC-EI-MS/MS. The positions of decarboxylation in the dicarboxylic and tricarboxylic acids were successfully identified by analyses using position-specific 13C-labeled standards prepared by in vitro enzymatic reactions. For example, carboxyl groups of C1 and C6 of trimethylsilyl (TMS)- and tert-butyldimethylsilyl (TBDMS)-derivatized malic and citric acids were primarily cleaved by EI. MS/MS analyses were also performed, and fragment ions of TBDMS-citric and α-ketoglutaric acids (αKG) with the loss of two carboxyl groups in collision-induced dissociation (CID) were observed.

著者

by Richard Blanke

出版者

Böhlau Verlag

巻号頁・発行日

2001

著者

Walther Hubatsch

出版者

N.G. Elwert

巻号頁・発行日

1966