著者

曽根 誠一

出版者

花園大学文学部

雑誌

花園大学文学部研究紀要 (ISSN:1342467X)

巻号頁・発行日

no.48, pp.73-85, 2016

著者

榎本 翔

出版者

情報知識学会

雑誌

情報知識学会誌 (ISSN:09171436)

巻号頁・発行日

vol.26, no.2, pp.221-226, 2016-05-14 (Released:2016-07-15)

参考文献数

12

---

近年,電子媒体の資料が増加している.研究者が資料を入手できるように,リンクリゾルバーやベンダーによるウェビナーの開催といった工夫が多く見られる.一方,大学図書館ではデータベースの代理検索や利用教育を行ってきたが,最新の動向は明らかになっていない.本研究では,日本の大学図書館におけるデータベース利用案内と利用教育がどのように行われているのか,複数の大学を抽出しWebサイトを通じて調査をし分析を行った.その結果,設置母体や大学の規模によって利用案内や利用教育の内容量に差が見られた.

著者

Sahara Ayako

出版者

Sophia University Institute of American and Canadian Studies

雑誌

The Journal of American and Canadian Studies (ISSN:09148035)

巻号頁・発行日

no.30, pp.55-84, 2013-03-31

著者

今西 美音子 佐野 友紀

出版者

日本建築学会

雑誌

日本建築学会計画系論文集 (ISSN:13404210)

巻号頁・発行日

vol.79, no.698, pp.917-922, 2014-04-30 (Released:2014-07-15)

参考文献数

23

被引用文献数

2 5

---

This study analyzes the pedestrian's avoidance behavior in crossing flow in order to obtain quantitative criteria for evaluating the difficulty of walking in a crowd. The new graphic illustration methods, called Short-Time Pedestrian Path Diagram and Direction Rose Diagram, are developed and proposed in this paper to represent the state of a crowd visually. The result of our experiment suggests that pedestrians descend their walking speed or/and detour to avoid striking other people and each avoidance behavior can be classified into three levels, whose threshold are defined from the speed or the angular velocity of each pedestrian.

著者

佐藤 一省 前田 法史 小川 範之

出版者

公益社団法人 化学工学会

雑誌

化学工学論文集 (ISSN:0386216X)

巻号頁・発行日

vol.21, no.3, pp.632-635, 1995-05-10 (Released:2009-11-12)

参考文献数

3

被引用文献数

1 1

---

The minimum power of impeller required for complete suspension of solid particles, based on Zwietering's criterion, was measured for single and twin impeller systems. The effects of the configuration of vessel and the number of impellers on the minimum required power were examined.The minimum required power per unit liquid volume Pfv for a single impeller system increased gradually as the length ratio of long side to short side A/B incresed over 1.5 Pfv for a twin impeller system remained nearly constant at a lower value than that of a single impeller system in the range of A/B=1.52.25.The Pfv of a pitched-blade turbine was below 1 / 2 of the value of a flat-blade disc turbine under the same conditions and was independent of the rotational direction of each impeller.

著者

佐竹 真次

出版者

山形県立保健医療大学

雑誌

山形保健医療研究 : 山形県立保健医療大学紀要 (ISSN:1343876X)

巻号頁・発行日

vol.18, pp.17-29, 2015-03

著者

レオナルド・ダ・ヴィンチ[著] 加藤朝鳥訳

出版者

北宋社

巻号頁・発行日

1996

著者

レオナルド・ダ・ヴィンチ [著] ジーン・ポール・リヒター編 杉田益次郎訳

出版者

アトリエ社

巻号頁・発行日

1941

著者

藤村 龍至 金野 千恵 伊庭野 大輔

出版者

日経BP社

雑誌

日経アーキテクチュア (ISSN:03850870)

巻号頁・発行日

no.1018, pp.57-59, 2014-02-25

---

図1は本特集で取り上げた100人の出身大学(最終学歴)を示したものだ。東京大学が21人で最も多かったが、同大学は他大学からの大学院進学者が少なくない。2位の早稲田大学は建築学科の学生数が国立大学に比べて多い。

著者

Oanh T. P. Kim Manh D. Le Hoang X. Trinh Hai V. Nong

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.173-180, 2016 (Released:2016-07-14)

参考文献数

42

被引用文献数

22

---

Tumor necrosis factor-alpha (TNF-α) is a cytokine that plays an important role in inflammatory process and tumor development. Recent studies demonstrate that triterpene saponins from Vietnamese ginseng are efficient inhibitors of TNF-α. But the interactions between TNF-α and the saponins are still unclear. In this study, molecular docking and molecular dynamics simulations of TNF-α with three different triterpene saponins (majonoside R2, vina-ginsenoside R1 and vina-ginsenoside R2) were performed to evaluate their binding ability. Our results showed that the triterpene saponins have a good binding affinity with protein TNF-α. The saponins were docked to the pore at the top of the “bell” or “cone” shaped TNF-α trimer and the complexes were structurally stable during 100 ns molecular dynamics simulation. The predicted binding sites would help to subsequently investigate the inhibitory mechanism of triterpene saponins.

著者

Hiroo Imai Nami Suzuki-Hashido Yoshiro Ishimaru Takanobu Sakurai Lijie Yin Wenshi Pan Masaji Ishiguro Katsuyoshi Masuda Keiko Abe Takumi Misaka Hirohisa Hirai

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.165-171, 2016 (Released:2016-07-14)

参考文献数

14

被引用文献数

1 5

---

In mammals, bitter taste is mediated by TAS2Rs, which belong to the family of seven transmembrane G protein-coupled receptors. Since TAS2Rs are directly involved in the interaction between mammals and their dietary sources, it is likely that these genes evolved to reflect species-specific diets during mammalian evolution. Here, we analyzed the amino acids responsible for the difference in sensitivities of TAS2R16s of various primates using a cultured cell expression system. We found that the sensitivity of TAS2R16 varied due to several amino acid residues. Mutation of amino acid residues at E86T, L247M, and V260F in human and langur TAS2R16 for mimicking the macaque TAS2R16 decreased the sensitivity of the receptor in an additive manner, which suggests its contribution to the potency of salicin, possibly via direct interaction. However, mutation of amino acid residues 125 and 133 in human TAS2R16, which are situated in helix 4, to the macaque sequence increased the sensitivity of the receptor. These results suggest the possibility that bitter taste sensitivities evolved independently by replacing specific amino acid residues of TAS2Rs in different primate species to adapt to species-specific food.

著者

Kazunori D. Yamada Hafumi Nishi Junichi Nakata Kengo Kinoshita

出版者

The Biophysical Society of Japan

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.157-163, 2016 (Released:2016-07-14)

参考文献数

41

被引用文献数

7

---

Functional sites on proteins play an important role in various molecular interactions and reactions between proteins and other molecules. Thus, mutations in functional sites can severely affect the overall phenotype. Progress of genome sequencing projects has yielded a wealth of information on single nucleotide variants (SNVs), especially those with less than 1% minor allele frequency (rare variants). To understand the functional influence of genetic variants at a protein level, we investigated the relationship between SNVs and protein functional sites in terms of minor allele frequency and the structural position of variants. As a result, we observed that SNVs were less abundant at ligand binding sites, which is consistent with a previous study on SNVs and protein interaction sites. Additionally, we found that non-rare variants tended to be located slightly apart from enzyme active sites. Examination of non-rare variants revealed that most of the mutations resulted in moderate changes of the physico-chemical properties of amino acids, suggesting the existence of functional constraints. In conclusion, this study shows that the mapping of genetic variants on protein structures could be a powerful approach to evaluate the functional impact of rare genetic variations.

著者

Masayuki Oda Takeshi Tsumuraya Ikuo Fujii

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.135-138, 2016 (Released:2016-07-14)

参考文献数

14

被引用文献数

2

---

We analyzed the correlation between the conformational strain and the binding kinetics in antigen-antibody interactions. The catalytic antibodies 6D9, 9C10, and 7C8 catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. The crystal structure of 6D9 complexed with a transition-state analog (TSA) suggests that 6D9 binds the substrate to change the conformation of the ester moiety to a thermodynamically unstable twisted conformation, enabling the substrate to reach the transition state during catalysis. The present binding kinetic analysis showed that the association rate for 6D9 binding to the substrate was much lower than that to TSA, whereas those for 9C10 and 7C8 binding were similar to those to TSA. Considering that 7C8 binds to the substrate with little conformational change in the substrate, the slow association rate observed in 6D9 could be attributed to the conformational strain in the substrate.

著者

Yuka Suzuki Kei Yura

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.127-134, 2016 (Released:2016-07-14)

参考文献数

26

被引用文献数

4

---

We investigated the effect of ATP binding to GroEL and elucidated a role of ATP in the conformational change of GroEL. GroEL is a tetradecamer chaperonin that helps protein folding by undergoing a conformational change from a closed state to an open state. This conformational change requires ATP, but does not require the hydrolysis of the ATP. The following three types of conformations are crystalized and the atomic coordinates are available; closed state without ATP, closed state with ATP and open state with ADP. We conducted simulations of the conformational change using Elastic Network Model from the closed state without ATP targeting at the open state, and from the closed state with ATP targeting at the open state. The simulations emphasizing the lowest normal mode showed that the one started with the closed state with ATP, rather than the one without ATP, reached a conformation closer to the open state. This difference was mainly caused by the changes in the positions of residues in the initial structure rather than the changes in “connectivity” of residues within the subunit. Our results suggest that ATP should behave as an insulator to induce conformation population shift in the closed state to the conformation that has a pathway leading to the open state.

著者

Georgios Iakovou Stephen Laycock Steven Hayward

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.97-103, 2016 (Released:2016-07-14)

参考文献数

37

被引用文献数

5

---

Interactive haptics-assisted docking provides a virtual environment for the study of molecular complex formation. It enables the user to interact with the virtual molecules, experience the interaction forces via their sense of touch, and gain insights about the docking process itself. Here we use a recently developed haptics software tool, Haptimol_RD, for the rigid docking of protein subunits to form complexes. Dimers, both homo and hetero, are loaded into the software with their subunits separated in space for the purpose of assessing whether they can be brought back into the correct docking pose via rigid-body movements. Four dimers were classified into two types: two with an interwinding subunit interface and two with a non-interwinding subunit interface. It was found that the two with an interwinding interface could not be docked whereas the two with the non-interwinding interface could be. For the two that could be docked a “sucking” effect could be felt on the haptic device when the correct binding pose was approached which is associated with a minimum in the interaction energy. It is clear that for those that could not be docked, the conformation of one or both of the subunits must change upon docking. This leads to the steric-based concept of a locked or non-locked interface. Non-locked interfaces have shapes that allow the subunits to come together or come apart without the necessity of intra-subunit conformational change, whereas locked interfaces require a conformational change within one or both subunits for them to be able to come apart.

著者

Kentaro Ishii Masanori Noda Susumu Uchiyama

出版者

一般社団法人 日本生物物理学会

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.13, pp.87-95, 2016 (Released:2016-07-14)

参考文献数

32

被引用文献数

32

---

The interactions of small molecules with proteins (protein–ligand interactions) mediate various biological phenomena including signal transduction and protein transcription and translation. Synthetic compounds such as drugs can also bind to target proteins, leading to the inhibition of protein–ligand interactions. These interactions typically accompany association–dissociation equilibrium according to the free energy difference between free and bound states; therefore, the quantitative biophysical analysis of the interactions, which uncovers the stoichiometry and dissociation constant, is important for understanding biological reactions as well as for rational drug development. Mass spectrometry (MS) has been used to determine the precise molecular masses of molecules. Recent advancements in MS enable us to determine the molecular masses of protein–ligand complexes without disrupting the non-covalent interactions through the gentle desolvation of the complexes by increasing the vacuum pressure of a chamber in a mass spectrometer. This method is called MS under non-denaturing conditions or native MS and allows the unambiguous determination of protein–ligand interactions. Under a few assumptions, MS has also been applied to determine the dissociation constants for protein–ligand interactions. The structural information of a protein–ligand interaction, such as the location of the interaction and conformational change in a protein, can also be analyzed using hydrogen/deuterium exchange MS. In this paper, we briefly describe the history, principle, and recent applications of MS for the study of protein–ligand interactions.

著者

外川 健一

出版者

九州大学

巻号頁・発行日

2002

---

博士論文

著者

水野 和彦 塩谷 繁 藤本 文弘

出版者

日本草地学会

雑誌

日本草地学会誌 (ISSN:04475933)

巻号頁・発行日

vol.43, no.3, pp.316-324, 1997-10-31

被引用文献数

6

---

オーチャードグラスの国内外の延べ16品種を対象に,5年間にわたり延べ17の農業形質を調査し,主にホルスタイン種育成雌牛で評価した嗜好性との関係を解析した。出穂期の嗜好性は品種の出穂茎率と高い相関を示した。栄養生長期の再生草の嗜好性は,その品種の手触りによる茎葉の柔軟性,耐病性(初夏の雲形病,夏の葉腐病,夏から秋のさび病)及び枯れ葉率と比較的高い相関を示し,これらの形質には品種間差異も存在したことから,今後嗜好性の選抜指標として有用と考えられた。葉の形態的形質(葉幅,葉厚,葉長及び鋸歯,毛茸)については,いずれも嗜好性との相関は認められなかった。高嗜好性品種Ludeは,柔軟性が高く,耐病性に優れていた。

著者

堀川 浩洋

出版者

日経BP社

雑誌

日経ビジネス (ISSN:00290491)

巻号頁・発行日

no.1488, pp.116-118, 2009-04-27

---

政府の経済対策の一環で、高速道路がどこまで走っても1000円だなんて、もうかないません。 瀬戸大橋の通行料が今年3月以降、大幅に安くなり、その影響で私たちのカーフェリーを利用して瀬戸内海を渡るお客さんが激減してしまいました。 私たちは、瀬戸内海を挟んで四国の高松港(香川県)と本州の宇野港(岡山県)を1時間で結ぶカーフェリーを1日往復で40便、運航しています。

著者

武田 周一郎

出版者

筑波大学人文社会科学研究科歴史・人類学専攻歴史地理学研究室

雑誌

歴史地理学野外研究 (ISSN:09152504)

巻号頁・発行日

no.17, pp.96-130, 2016-03