著者

Shunta Kihara Kosuke Yamamoto Atsushi Hisatomi Yuh Shiwa Chia-Cheng Chu Kanako Takada Michel Ouyabe Babil Pachakkil Hidehiko Kikuno Naoto Tanaka Hironobu Shiwachi

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.37, no.2, pp.ME21062, 2022 (Released:2022-05-03)

参考文献数

53

被引用文献数

3

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The bacterial community of water yam (Dioscorea alata L.) cv. A-19 is vital because it may promote plant growth without the need for fertilization. However, the influence of fertilization practices on the composition and proportion of the bacterial community of water yam cv. A-19 has not yet been extensively examined. Therefore, we herein investigated the diversity and composition of the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization using amplicon community profiling based on 16S rRNA gene sequences. No significant difference was detected in the growth of plants cultivated with or without chemical fertilization. Alpha diversity indices were significantly dependent on each compartment, and a decrease was observed in indices from the belowground (rhizosphere and root) to aboveground compartments (stem and leaf). The bacterial composition of each compartment was clustered into three groups: bulk soil, rhizosphere and root, and stem and leaf. Chemical fertilization did not significantly influence the diversity or composition of the water yam cv. A-19 bacterial community. It remained robust in plants cultivated with chemical fertilization. The amplicon community profiling of bacterial communities also revealed the dominance of two bacterial clades, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Burkholderia-Caballeronia-Paraburkholderia clade, with and without chemical fertilization. This is the first study to characterize the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization.

著者

Yusuke Makino Megumi Ueno Yoshimi Shoji Minako Nyui Ikuo Nakanishi Koji Fukui Ken-ichiro Matsumoto

出版者

SOCIETY FOR FREE RADICAL RESEARCH JAPAN

雑誌

Journal of Clinical Biochemistry and Nutrition (ISSN:09120009)

巻号頁・発行日

pp.21-83, (Released:2021-12-25)

参考文献数

9

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The quantitative measurement of free radicals in liquid using an X-band electron paramagnetic resonance (EPR) was systematized. Quantification of free radicals by EPR requires a standard sample that contains a known spin amount/concentration. When satisfactory reproducibility of the sample material, volume, shape, and positioning in the cavity for EPR measurements can be guaranteed, a sample tested and a standard can be directly compared and the process of quantification can be simplified. The purpose of this study was to simplify manual quantitative EPR measurement. A suitable sample volume for achieving a stable EPR intensity was estimated. The effects of different solvents on the EPR sensitivity were compared. The stability and reproducibility of the EPR intensity of standard nitroxyl radical solutions were compared among different types of sample tubes. When the sample tubes, sample volumes, and/or solvents were the same, the EPR intensity was reproduced with an error of 2% or less for μM samples. The quantified sample and the standard sample in the same solvent and the same volume drawn into the same sample tube was able to be directly compared. The standard sample for quantification should be measured just before or after every daily experiment.

著者

Risa Tamagawa-Mineoka Koji Masuda Akiko Yagami Masashi Nakamura Nayu Sato Kayoko Matsunaga Norito Katoh

出版者

Japanese Society of Allergology

雑誌

Allergology International (ISSN:13238930)

巻号頁・発行日

vol.67, no.3, pp.427-429, 2018 (Released:2018-07-28)

参考文献数

13

被引用文献数

8

著者

Kwan-Woo Lee Won-Ho Kim

出版者

The Society of Physical Therapy Science

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.28, no.1, pp.175-180, 2016 (Released:2016-01-30)

参考文献数

40

被引用文献数

8 19

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[Purpose] To investigate the effects of thoracic manipulation and deep craniocervical flexor training on the muscle strength and endurance, range of motion, and the disability index of the neck of patients with chronic nonspecific neck pain. [Subjects and Methods] Forty-six patients with chronic neck pain participated. They received an intervention for 35 minutes a day, three times a week for 10 weeks. Subjects were randomly assigned to one control and two experimental groups: group A (thoracic manipulation combined with deep craniocervical flexor training, n=16), group B (deep craniocervical flexor training, n=15), and group C (active self-exercise as a control group, n=15). Muscle strength and endurance, pain, neck disability index, and range of motion of the cervical and thoracic spine were measured before and after the intervention. [Results] Group A showed significant increases in muscle strength, endurance, and cervical and thoracic range of motion, and significant decreases in the pain and neck disability index, compared with groups B and C. [Conclusion] Although deep craniocervical flexor training is effective at improving neck function, thoracic manipulation combined with deep craniocervical flexor training was a more effective intervention for pain relief and improving the range of motion, muscle function, and neck disability of patients with nonspecific chronic neck pain.

著者

Ting-Ting HU Chun-Mei LU Han LI Zai-Xiao ZHANG Yun-Hui ZHAO Juan LI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.9, pp.1027-1032, 2017-09-10 (Released:2017-09-10)

参考文献数

28

被引用文献数

11 18

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The overuse of organophosphorus pesticides on cotton production is a big concern in China today. Therefore, developing methods for the rapid screening and confirming of pesticide residues in textiles has become a top public health security priority. Here, a method was established for the rapid screening and quantifying of 11 kinds of organophosphorus pesticides (ethoprophos, coumaphos, profenofos, diazinon, ethion, parathion, phosalone, quinalphos, dicrotophos, azinphos methyl, and tribuphos) in textiles by high-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS). Rapid screening and quantifying could be completed by using software of Peakview and MultiQuant. Samples were extracted by the method of modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and analyzed in the positive mode with MS detection. The results showed that the limits of detection were between 0.1 and 5.0 ng g−1, with correlation coefficients above 0.9990. The recoveries were in the range of 70.3 – 109.8%, with relative standard deviations from 5.1 to 16.4%. This method is accurate and simple, which can be used in the rapid screening and quantitative analysis of 11 kinds of organophosphorus pesticides in textiles.

著者

横谷 明徳 渡辺 立子 秋光 信佳 岡 壽崇 鵜飼 正敏 福永 久典 藤井 健太郎 服部 佑哉 野口 実穂 泉 雄大 Hervé du Penhoat Marie-Anne

出版者

国立研究開発法人量子科学技術研究開発機構

雑誌

基盤研究(B)

巻号頁・発行日

2015-04-01

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X線照射したEGFPプラスミドを“非照射”の細胞導入し、ライブセル観察によりEGFP蛍光の発現速度の低下から難修復性のクラスターDNA損傷が生じていることを示した。また軟X線を照射しながら水和デオキシリボース(dR)からの脱離イオンを測定し、水分子が分子の激しい分解を抑制すること、またその理由がdRから配位水への高速のプロトン移動によることを分子動力学計算により示した。さらに放射線トラックエンドで生じる多数の低速2次電子は、発生位置から数nm以上離れたところに塩基損傷を誘発し、修復過程を経てDNAの2本鎖切断に変換され得るクラスター損傷を生成することを示した。

著者

Fernando LÓPEZ-GATIUS

出版者

The Society for Reproduction and Development

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

vol.67, no.2, pp.135-139, 2021 (Released:2021-04-21)

参考文献数

33

被引用文献数

4

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Since the 1970s, luteolytic doses used for synchronizing estrus in dairy cattle have remained unchanged. This study aimed to evaluate the dose-response effect of prostaglandin F2α (PGF2α), which is used for synchronizing estrus, and subsequent fertility in cows with two or more corpora lutea (CL). The study population consisted of 1,683 cows with a single CL (1CL), 501 cows with multiple CL receiving a single dose of PGF2α (2CL1), and 252 cows with multiple CL receiving a 1.5 × PGF2α dose (2CL1.5). Cows with a single CL (n = 1,245) showed estrus significantly (P < 0.01) earlier (3.01 ± 1.23 days; mean ± SD) than cows with multiple CL (n = 287; 3.33 ± 1.69 days). Using 1CL cows as reference, the odds ratio (OR) for the estrus response in 2CL1 cows was 0.13 (P < 0.0001), whereas the ORs for estrus response and pregnancy of 2CL1.5 cows were 1.8 (P = 0.0001) and 1.7 (P = 0.001), respectively. Based on the results for only the 2CL1 cows, the OR for the estrus response was 0.7 (P = 0.01) for cows producing ≥ 45 kg of milk at treatment, compared to the remaining cows producing < 45 kg of milk. Our results showed that the presence of multiple CL reduced the estrus response to that induced by a single PGF2α dose and milk production was inversely associated with this response, whereas an increased PGF2α dose improved the estrus response. Therefore, an increase in the standard PGF2α dose is recommended.

著者

Yasuda Shumpei P Vogel Peter Tsuchiya Kimiyuki Han Sang-Hoon Lin Liang-Kong Suzuki Hitoshi

出版者

National Research Council Canada

雑誌

Canadian Journal of Zoology (ISSN:00084301)

巻号頁・発行日

vol.83, no.11, pp.1411-1420, 2005-11

被引用文献数

50

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We examined sequence variation in the mitochondrial cytochrome b gene (1140 bp, n = 73) and control region (842–851 bp, n = 74) in the Eurasian harvest mouse (Micromys minutus (Pallas, 1771)), with samples drawn from across its range, from Western Europe to Japan. Phylogeographic analyses revealed region-specific haplotype groupings combined with overall low levels of inter-regional genetic divergence. Despite the enormous intervening distance, European and East Asian samples showed a net nucleotide divergence of only 0.36%. Based on an evolutionary rate for the cytochrome b gene of 2.4%·(site·lineage·million years)–1, the initial divergence time of these populations is estimated at around 80 000 years before present. Our findings are consistent with available fossil evidence that has recorded repeated cycles of extinction and recolonization of Europe by M. minutus through the Quaternary. The molecular data further suggest that recolonization occurred from refugia in the Central to East Asian region. Japanese haplotypes of M. minutus, with the exception of those from Tsushima Is., show limited nucleotide diversity (0.15%) compared with those found on the adjacent Korean Peninsula. This finding suggests recent colonization of the Japanese Archipelago, probably around the last glacial period, followed by rapid population growth.

著者

Victor Benno Meyer-Rochow Carsten H. G. Müller Magnus Lindström

出版者

JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY

雑誌

Applied Entomology and Zoology (ISSN:00036862)

巻号頁・発行日

vol.41, no.1, pp.117-122, 2006 (Released:2006-04-10)

参考文献数

33

被引用文献数

6 8

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In order to solve the long-standing problem as to whether the eyes of Scutigera coleoptrata were functional and responded to flashes of light, we carried out electrophysiological recordings from them with glass electrodes pushed into the eye's retina. The electroretinograms were typical cornea-negative responses, similar in shape and duration to those seen in other arthropods, including those with cryptic lifestyles. We located two sensitivity peaks: one in the vicinity to light of 448 nm wavelength and a second 0.25–0.5 log unit lower one in the ultraviolet region (UV) of the spectrum around 350 nm and we expect at least two kinds of visual pigments to be present. We can only speculate on the surprisingly well-developed UV-sensitivity and suggest that it could serve the animal as an alarm colour to avoid open, illuminated spaces or to detect exits from concealed hiding places in soil crevices and from under boulders.

著者

Fumiko Kihara-Negishi Naoki Ohkura Yuka Takahashi Toshiyuki Fujita Yasuhiro Nakamura Keiji Maruyama Tetsuta Oshitari Shinji Yamaguchi

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.45, no.4, pp.528-533, 2022-04-01 (Released:2022-04-01)

参考文献数

40

被引用文献数

4

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Brown adipose tissue (BAT) specifically regulates energy expenditure via heat production. Nobiletin (NOB), a natural polymethoxylated flavone present in citrus fruits, can activate thermogenesis in the BAT of high-fat diet-induced obese mice. The activity of BAT is directly regulated by β-adrenergic stimulation. In this study, we report the effects of NOB on BAT activation using β-adrenergic agonists. We observed that when HB2 brown adipocyte cell lines are stimulated with β-adrenergic agonists, NOB enhances the expression of uncoupling protein 1 (UCP1), which is associated with the mitochondrial energy metabolism in these cells. Moreover, NOB increases the mRNA expression of the brown adipokines neuregulin-4 (Nrg4) and fibroblast growth factor-21 (FGF-21) and the secretion of FGF-21. These results suggest that NOB can enhance the thermogenic functions of brown adipocytes and promote brown adipokine secretion due to enhanced β-adrenergic stimulation. In addition, 3′-demethyl nobiletin (3′-DMN), an NOB CYP-enzyme metabolite, can increase UCP1 mRNA expression. Both NOB and 3′-DMN significantly promoted mitochondrial membrane potential in HB2 adipocytes following β-adrenergic stimulation. Therefore, we believe that NOB could be a promising candidate for activating BAT under β-adrenergic stimulation and preventing the onset of obesity.

著者

渡邊 裕也 山田 陽介 吉田 司 横山 慶一 三宅 基子 山縣 恵美 山田 実 吉中 康子 木村 みさか Kyoto-Kameokaスタディグループ

出版者

日本運動疫学会

雑誌

運動疫学研究 (ISSN:13475827)

巻号頁・発行日

pp.2014, (Released:2020-12-08)

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背景:長寿社会において,最も深刻な社会問題の1つにサルコペニアおよびフレイルがある。高齢者の自立と生活の質を維持するためには,これらを予防することが重要である。本研究では,地域在住高齢者を対象に,自己管理式の包括的介護予防プログラム(Comprehensive geriatric intervention program:CGIP)が身体機能および骨格筋量に及ぼす効果を調査した。我々は,CGIPを自宅で実施する群(自宅型)と自宅での実施に加えて週に一度の集団指導を行う群(教室型)の介入効果を比較した 。 方法:526名の参加者を,居住地区に基づいて2群(教室型 251名,自宅型275名)のいずれかに無作為に割り付けた。低負荷レジスタンストレーニング,身体活動量の増加,口腔機能の改善,栄養ガイドで構成されるCGIPを12週間実施した。参加者全員に,プログラムの説明を含む90分の講義を2回受講するよう促した。参加者にはトレーニングツール(3軸加速度内蔵活動量計,アンクルウエイト,ゴムバンド)と日誌が提供された。教室型介入群は毎週90分のセッションに参加し,その他の日には自身でプログラムを実施した。一方,自宅型介入群はプログラム実施方法の説明のみを受けた。12週間の介入前後に,膝伸展筋力,通常および最大歩行速度,Timed up and go(TUG)テスト,大腿前部筋組織厚などの身体機能を測定し,Intention-to-treat法を用いて分析した。 結果:526名の高齢者のうち,517名(教室型243名 74.0±5.4歳 女性57.2%,自宅型274名 74.0±5.6 女性58.8%)が研究対象として組み入れられた。9名(教室型 8名,自宅型 1名)は介入前の測定に参加していなかったため,解析から除外された。いずれの介入も膝伸展筋力(教室型18.5%,自宅型10.6%),正常歩行速度(教室型3.7%,自宅型2.8%),大腿前部筋組織厚(教室型3.2%,自宅型3.5%)を有意に改善した。なお,膝伸展筋力は教室型でより大きな改善が認められた(P=0.003)。最大歩行速度(教室型 4.7%,自宅型1.8%,P=0.001)およびTUGテスト(教室型 -4.7%,自宅型 -0.2%,P<0.001)は教室型介入群のみで有意に改善した。 結論:本介入プログラムはサルコペニア,フレイルの予防に有効であった。両介入後,ほとんどの身体機能と大腿前部筋組織厚は改善した。自宅型介入は費用対効果が高く,大規模高齢者集団におけるサルコペニア,フレイルの予防に貢献できるかもしれない。

著者

Chika Takai-Yamashita Emiko Sato Masayoshi Fuji

出版者

Hosokawa Powder Technology Foundation

雑誌

KONA Powder and Particle Journal (ISSN:02884534)

巻号頁・発行日

vol.37, pp.233-243, 2020-01-10 (Released:2020-02-29)

参考文献数

37

被引用文献数

7 15

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The NMR-based solvent relaxation technique, a non-invasive tool to characterize the surface of particles, which are dispersed in a liquid, was applied to characterize the nanoparticles’ aggregation structure. The liquid molecules in a dispersion undergo a rapid exchange between the bound states at the interface and highly mobile free states in a bulk liquid. The relaxation time of the liquid molecules bound on the particle surface is shorter than that of the free states liquid. By detecting how much liquid is bound on the particle surface, the wetted specific surface area (SNMR) can be determined. In this study, it was clarified that the water adsorbed at more than a 1.138 layer from the silica surface can be detected by the NMR and the maximum limitation ranged from 2.160 and 3.336 layers. The model aggregates with an artificial solid neck among the particles were mixed with the silica nanoparticle dispersion. Although the determined SNMR was underestimated compared to SBET from gas adsorption, even a low ratio (5 mass%) of the model aggregates in the dispersion can be detected.

著者

Yoshinori FURUKAWA Ken NAGASHIMA Etsuro YOKOYAMA Shunichi NAKATSUBO Salvador ZEPEDA Izumi YOSHIZAKI Haruka TAMARU Taro SHIMAOKA Takehiko SONE Takao MAKI Asuka YAMAMOTO Toshiyuki TOMOBE Ken-ichiro MURATA Gen SAZAKI

出版者

The Japan Society of Microgravity Application

雑誌

International Journal of Microgravity Science and Application (ISSN:21889783)

巻号頁・発行日

vol.38, no.1, pp.380101, 2021 (Released:2021-01-31)

参考文献数

53

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Microgravity experiments on ice crystal growth in supercooled water without any effect of convection flow were carried out twice in Kibo of the International Space Station as projects called “Ice Crystal 1” and “Ice Crystal 2”. Many video movies recording the growth process of a single ice crystal were analyzed to clarify the pattern formation processes of single ice crystals and the impurity effect on ice crystal growth kinetics. In the Ice Crystal 1 project, ice crystals were grown in supercooled pure D2O and the pattern formation mechanism for the ice crystal was clarified on the basis of crystal plane-dependent growth kinetics between the basal face and the other crystallographic faces. In the Ice Crystal 2 project, ice crystals were grown in supercooled H2O containing a small amount of an antifreeze glycoprotein as an impurity. Growth enhancement and oscillation for the basal faces were observed for the first time, and their mechanisms were clarified on the basis of the crystal plane-dependent impurity effect of an antifreeze glycoprotein. The origin of the antifreeze function of antifreeze glycoproteins was ultimately explained in connection with the basic criterion for the pattern formation of a polyhedral crystal.

著者

Jiyoung PARK Aryung NAM Hae-Beom LEE Seong Mok JEONG Dae-Hyun KIM

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0673, (Released:2022-04-15)

被引用文献数

1

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A 10-year-old intact male Shih Tzu dog presented with hematuria. Double-contrast cystography revealed a polypoid filling defect at the apex of the urinary bladder. Ultrasonography revealed a heterogeneously hypoechoic intramural mass with minimal vascular flow beneath the submucosal layer. After partial cystectomy, a well-demarcated bladder leiomyosarcoma was diagnosed on histopathology. The patient was alive and well without any clinical signs, recurrence, or metastasis at the 29-month follow-up after the surgical removal of the bladder mass. Leiomyosarcoma should be considered as a differential diagnosis if mass-like lesions are observed in the urinary bladder, although this type of malignancy is rare in canines. Histopathological confirmation is important for predicting prognosis and determining further medical plans.

著者

Hsi-Tien Wu Ya-Wen Chuang Cheng-Pu Huang Ming-Huang Chang

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.67, no.1, pp.41-49, 2018 (Released:2018-02-09)

参考文献数

38

被引用文献数

14 20

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Angiotensin converting enzyme II (ACE2), an angiotensin converting enzyme (ACE) homologue that displays antagonist effects on ACE/angiotensin II (Ang II) axis in renin-angiotensin system (RAS), could play a protective role against liver damages. The purpose of this study is to investigate whether inflammation-mediated liver injury could be affected by ACE2 derived pathways in the RAS. Eight-weeks-old wild-type (WT; C57BL/6) and Ace2 KO (hemizygous Ace2-/y) male mice were used to induce liver fibrosis by thioacetamide (TAA) administration (0, 100, and 200 mg/kg BW). The mice administrated with TAA could be successfully induced liver fibrosis in a TAA-dose dependent manner. Compared to WT mice, the results show that Ace2 KO mice have high sensitive, and developed more serious reaction of hepatic inflammation and fibrosis by TAA administration. The physiological and pathological examinations demonstrated higher serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels, infiltration of white blood cells and fibrotic lesions within liver in the Ace2 KO mice. The severe liver damage of Ace2 KO mice were also confirmed by the evidence of higher expression of hepatic inflammation-related genes (IL-6 and Tnf) and fibrosis-related genes (Col1a1, Timp1 and Mmp9). Ace2 gene deficiency could lead to a severe inflammation and collagen remodeling in the liver administrated by TAA, and the responses lead the pathogenesis of liver fibrosis. Our studies provided the main messages and favorable study directions of relationship of Ace2 and liver disease.

著者

Shin SHIMADA Takahiro YOSHIZAWA Yuki TAKAHASHI Yuko NITAHARA-KASAHARA Takashi OKADA Yoshihiro NOMURA Hitoki YAMANAKA Tomoki KOSHO Kiyoshi MATSUMOTO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.69, no.4, pp.407-413, 2020 (Released:2020-11-12)

参考文献数

19

被引用文献数

2 3

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Ehlers–Danlos syndromes (EDSs) are heterogeneous group of heritable connective tissue disorders characterized by joint and skin hyperextensibility as well as fragility of various organs. Recently, we described a new type of EDS, musculocontractual EDS (mcEDS-CHST14), caused by pathogenic variants of the carbohydrate sulfotransferase 14 (CHST14) gene mutation. B6;129S5-Chst14tm1Lex/Mmucd (B6;129-Chst14 KO) mice are expected to be an animal model of mcEDS-CHST14. However, >90% of B6;129-Chst14 KO homozygous (B6;129-Chst14−/−) mice show perinatal lethality. Therefore, improvement of the birth rate of Chst14−/− mice is needed to clarify the pathophysiology of mcEDS-CHST14 using this animal model. Some B6;129-Chst14−/− embryos had survived at embryonic day 18.5 in utero, suggesting that problems with delivery and/or childcare may cause perinatal lethality. However, in vitro fertilization and egg transfer did not improve the birth rate of the mice. A recent report showed that backcrossing to C57BL/6 strain induces perinatal death of all Chst14−/− mice, suggesting that genetic background influences the birthrate of these mice. In the present study, we performed backcrossing of B6;129-Chst14 KO mice to a BALB/c strain, an inbred strain that shows lower risks of litter loss than C57BL/6 strain. Upon backcrossing 1 to 12 times, the birth rate of Chst14−/− mice was improved with a birth rate of 6.12–18.64%. These results suggest that the genetic background influences the birth rate of Chst14−/− mice. BALB/c congenic Chst14−/− (BALB.Chst14−/−) mice may facilitate investigation of mcEDS-CHST14. Furthermore, backcrossing to an appropriate strain may contribute to optimizing animal experiments.

著者

濵園 侑美 Marrese-Taylor Edison 石垣 達也 宮尾 祐介 小林 一郎 高村 大也

雑誌

2022年度 人工知能学会全国大会(第36回)

巻号頁・発行日

2022-04-07

著者

Yoshio KIKU Yuya NAGASAWA Fuyuko TANABE Kazue SUGAWARA Atsushi WATANABE Eiji HATA Tomomi OZAWA Kei-ichi NAKAJIMA Toshiro ARAI Tomohito HAYASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.78, no.9, pp.1505-1510, 2016 (Released:2016-10-01)

参考文献数

27

被引用文献数

15 18

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Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations. Three of these genes encode chemokines (IL-8, CXCL6 and CCL2) functioning as chemoattractant molecules for neutrophils and macrophages. These results suggest that the initial inflammatory response of SA infection in mammary gland may be related with LTA induced chemokine genes.

著者

Michiko Takahashi Kei Wada Syun-ichi Urayama Yuichi Masuda Keizo Nagasaki

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.37, no.5, pp.ME21075, 2022 (Released:2022-04-09)

参考文献数

26

被引用文献数

2

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Heterocapsa circularisquama RNA virus (HcRNAV) is the only dinoflagellate-infecting RNA virus that has been isolated to date. We herein investigated the diversity of the major capsid protein gene of HcRNAV and related viruses using degenerate PCR and in silico ana­lyses. Diverse sequences related to HcRNAV were successfully amplified from marine sediments. Amplicons contained conserved and variable regions; the latter were predicted to be located on the outer surface of the capsid. Our approach provides insights into the diversity of viruses that are difficult to isolate in the environment and will enhance rapidly growing metagenome sequence repositories.

著者

Ken Okumura Hirofumi Tomita Michikazu Nakai Eitaro Kodani Masaharu Akao Shinya Suzuki Kenshi Hayashi Mitsuaki Sawano Masahiko Goya Takeshi Yamashita Keiichi Fukuda Hisashi Ogawa Toyonobu Tsuda Mitsuaki Isobe Kazunori Toyoda Yoshihiro Miyamoto Hiroaki Miyata Tomonori Okamura Yusuke Sasahara for the J-RISK AF Research Group

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.85, no.8, pp.1254-1262, 2021-07-21 (Released:2021-07-21)

参考文献数

22

被引用文献数

1 15

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Background:Recently, identification of independent risk factors for ischemic stroke in Japanese non-valvular atrial fibrillation (NVAF) patients was made by analyzing the 5 major Japanese registries: J-RHYTHM Registry, Fushimi AF Registry, Shinken Database, Keio interhospital Cardiovascular Studies, and the Hokuriku-Plus AF Registry.Methods and Results:The predictive value of the risk scheme in Japanese NVAF patients was assessed. Of 16,918 patients, 12,289 NVAF patients were analyzed (mean follow up, 649±181 days). Hazard ratios (HRs) of each significant, independent risk factor were determined by using adjusted Cox-hazard proportional analysis. Scoring system for ischemic stroke was created by transforming HR logarithmically and was estimated by c-statistic. During the 21,820 person-years follow up, 241 ischemic stroke events occurred. Significant risk factors were: being elderly (aged 75–84 years [E], HR=1.74), extreme elderly (≥85 years [EE], HR=2.41), having hypertension (H, HR=1.60), previous stroke (S, HR=2.75), type of AF (persistent/permanent) (T, HR=1.59), and low body mass index <18.5 kg/m2(L, HR=1.55) after adjusting for oral anticoagulant treatment. The score was assigned as follows: 1 point to H, E, L, and T, and 2 points to EE and S (HELT-E2S2score). The C-statistic, using this score, was 0.681 (95% confidence interval [CI]=0.647–0.714), which was significantly higher than those using CHADS2(0.647; 95% CI=0.614–0.681, P=0.027 for comparison) and CHA2DS2-VASc scores (0.641; 95% CI=0.608–0.673, P=0.008).Conclusions:The HELT-E2S2score may be useful for identifying Japanese NVAF patients at risk of ischemic stroke.