著者

Reeko SATO Saori KOBAYASHI Yuya ABE Hiroaki KAMISHINA Shinichi Oda Jun YASUDA Juso SASAKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1204140815, (Released:2012-04-24)

被引用文献数

1

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This study reported detailed clinical effects of bovine lactoferrin on two canine littermates (1 female and 1 male) with familial neutrophil dysfunction and an investigation of their genetic background. Clinical signs caused by severe upper respiratory bacterial infections were observed in these dogs. Oral administration of bovine lactoferrin for a long duration improved their clinical signs (severe uveitis in the female dog and coughing from pneumonia in the male dog). Their backcross dogs that have the same father didn't show clinical signs of bacterial infection. Neutrophil function tests revealed that the backcross dogs didn't have any disorders. It is likely that abnormal clinical signs are associated with neutrophil dysfunction in the colony and the mother dog of these cases might be the genetic carrier of this dysfunction.

著者

Seong-Sung KWAK Seung-A CHEONG Yubyeol JEON Sang-Hwan HYUN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1204140816, (Released:2012-04-24)

被引用文献数

14 16

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We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNT-derived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (P<0.05) increased blastocyst formation and total cell number in blastocysts compared to the control (12.3% and 41.4 vs. 9.0% and 34.7, respectively). However, there was no effect on cleavage rate. The number of cells in the inner cell mass and trophectoderm were significantly higher in the pGM-CSF treatment group (6.0 and 43.0, respectively) compared to the control (4.4 and 31.9, respectively). Treatment with 10 ng/ml pGM-CSF significantly increased POU5F1 and Cdx2 mRNA expression in blastocysts. In addition, Bcl-2, Dnmt1, and proliferating cell nuclear antigen (PCNA) mRNA expression were up-regulated in blastocysts in the pGM-CSF supplemented group compared to the control. These results suggest that pGM-CSF improves the quality and developmental viability of porcine SCNT embryos by regulating transcription factor expression.

著者

林 英夫

出版者

日本行動計量学会

雑誌

行動計量学 (ISSN:03855481)

巻号頁・発行日

vol.37, no.2, pp.127-145, 2010 (Released:2011-01-22)

参考文献数

98

被引用文献数

2 1

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The main purpose of this article is to provide a diverse range of positive evidence that refutes groundless arguments against mail surveys. A versatile set of methodological studies are employed to clarify key factors: return rate, length of time required to conduct surveys, cost requirements, answers by proxy respondents, and ability to obtain straightforward answers. Consequently, the author shows that mail surveys are recognized by most respondents as a survey method that is easy to respond to, simple to participate in, and convenient for filling out and submitting a questionnaire. Furthermore, this work introduces several successful cases of converting methodology from personal interviews or drop-off/pick-up surveys to mail surveys, as well as using these methods together. In particular, the author emphasizes the need for further discussion to clarify the definitions of technical terms, to concretely describe the survey processes used for research papers, and to eventually standardize the quality of survey results so that systematic reviews can be conducted sometime in the very near future.

著者

大畑 健次 小西 慶幸 瀬藤 光利

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.50, no.2, pp.080-083, 2010 (Released:2010-03-30)

参考文献数

15

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Imaging mass spectrometry (IMS) visualizes the distribution of a wide range of biomolecules in cells and tissue sections. IMS can analyze a variety of biomolecules including unexpected metabolites, without labels in a non-targeted way. We developed AP-MALDI (atmospheric pressure matrix assisted laser desorption/ionization) which is equipped with an atmospheric pressure ion-source chamber. It allows us to analyze flesh samples with minimal loss of intrinsic water or volatile compounds.

著者

Kensuke Ishitani

出版者

The Japan Society for Industrial and Applied Mathematics

雑誌

JSIAM Letters (ISSN:18830609)

巻号頁・発行日

vol.4, pp.13-16, 2012 (Released:2012-04-16)

参考文献数

6

被引用文献数

5 5 2

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This paper presents a new methodology to compute VaR in the portfolio credit loss model. The Wavelet Approximation can be useful to compute non-smooth distributions, often arising in small or concentrated portfolios. We contribute to this technique by extending the Wavelet Approximation for Vasicek one-factor model to multi-factor model. Key features of our new algorithm are: (i) a finite series expansion of the wavelet scaling coefficients, (ii) Wynn's epsilon-algorithm to accelerate convergence of those series, and (iii) an efficient spline interpolation to calculate the Laplace transforms. We illustrate the effectiveness of our algorithm through numerical examples.

著者

石川 裕

出版者

社団法人 プラズマ・核融合学会

雑誌

プラズマ・核融合学会誌 (ISSN:09187928)

巻号頁・発行日

vol.79, no.8, pp.759-764, 2003 (Released:2005-09-28)

参考文献数

4

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This paper introduces an overview of the SCore cluster system software running on the Linux operating system. SCore utilizes PC cluster hardware efficiently and provides its users a high-performance parallel programming environment. This paper also presents brief installation information for the end-users.

著者

佐藤 大樹

出版者

日本菌学会

雑誌

日本菌学会大会講演要旨集 日本菌学会第55回大会

巻号頁・発行日

pp.38, 2011 (Released:2012-02-23)

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2011年4月から5月にかけて筑波山中腹の3箇所の沢でフタバコカゲロウ(Baetiella japonica)を採集した.このカゲロウは大きめの石や岩盤の表面にしがみついており,左手で水をよけ,現れた幼虫を鋭利なピンセットでつまみとり氷冷して研究室に持ち帰った.採集された複数の個体において,肛門から伸び出た菌糸が観察された.5月23日に採集された個体では,21頭中4頭が肛門から菌糸を伸ばしていた.菌体の基部は半球形に寄主の後腸クチクラに食い込んでおり,周囲は褐色を呈していた.菌体は主軸があり,肛門から伸び出した後に隔壁部分から一箇所あたり2-3本に分枝し,これを繰り返した.菌糸先端付近は,6-14μm間隔で隔壁に区切られた胞子形成細胞を形成し,続いて斜めにトリコスポア(trichospore:離脱生の胞子嚢)を形成した.トリコスポアは細長い円筒形でカラーはなく,長さ50-62.4μm幅4.0-4.6μmであった.同様の菌体基部,分枝様式を持った菌体において,菌糸の先端部分が癒合し,中間に紡錘形の接合子の形成が認められた(高さ8.0-9.4μm幅38.0-42.0μm).水生昆虫の肛門から菌体が伸び出すトリコミケテス類は,レゲリオミケス科(Legeriomycetaceae) のOrphella, Pteromaktron, Zygopolarisの3属である.Orphella属とは,トリコスポアの形態が異なり,Pteromaktronとは分枝のタイプが異なる.Zygoporarisとは,トリコスポア形成様式が似ているが,接合子の形態が異なる.従って,本属を新属と判断した.トリコミケテス類は観察に適した良い状態の標本を採集することが困難である場合が多いが,今回は,胞子形成が始まったばかりの菌体をスライドガラス上に1滴の沢の水とともに5℃で追培養を行い,トリコスポアの形成過程を約10日間連続観察できた.レゲリオミケス科のように分枝する菌体を持つトリコミケテス類は,分離培養までは達成できなくても,追培養による形態形成観察は有用な手段であると考えられた.

著者

若林 一郎

出版者

Osaka Urban Living and Health Association

雑誌

生活衛生 (ISSN:05824176)

巻号頁・発行日

vol.48, no.1, pp.387-395, 2004 (Released:2005-01-27)

参考文献数

60

被引用文献数

1

著者

下瀬川 徹 伊藤 鉄英 中村 太一 宮川 宏之 中村 光男 丹藤 雄介 廣田 衛久 佐藤 晃彦 神澤 輝実 清水 京子 佐田 尚宏 丸山 勝也 大原 弘隆 成瀬 達 石黒 洋 片岡 慶正 保田 宏明 大野 隆真 五十嵐 久人 木原 康之 山口 貞子 村上 裕子 畑迫 実葉香 山雄 健次 乾 和郎 峯 徹哉

出版者

一般社団法人 日本膵臓学会

雑誌

膵臓 (ISSN:09130071)

巻号頁・発行日

vol.25, no.6, pp.617-681, 2010 (Released:2011-01-27)

参考文献数

142

被引用文献数

8 3

著者

渡辺 法男 安村 幹央 中川 千草 立山 健一郎 安田 公夫

出版者

日本緩和医療学会

雑誌

Palliative Care Research (ISSN:18805302)

巻号頁・発行日

vol.2, no.2, pp.310-312, 2007 (Released:2007-11-07)

参考文献数

10

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【目的】オピオイド鎮痛薬は単剤治療が基本であるが, モルヒネとフェンタニルパッチ (FP) を併用することにより, 疼痛, 咳嗽・呼吸困難の症状緩和を得ることができた症例を経験したので報告する. 【症例】50歳代, 女性, S状結腸がん, 肝・肺転移. 疼痛に加え咳嗽・呼吸困難があったため, 硫酸モルヒネを開始し, 症状緩和を得た. 将来的に経口摂取が困難になることを考慮し, FPへの変更を計画した. しかしながら, フェンタニルの咳嗽・呼吸困難に対する効果は確立していないため, 咳嗽・呼吸困難に対しては少量の硫酸モルヒネを継続し, 疼痛に対してはFPにて調節を行い, 最後までモルヒネとフェンタニルの両成分を併用することにより, 疼痛, 咳嗽・呼吸困難の症状緩和を得ることができた. 【結論】経口摂取困難で, 疼痛に加え, 咳嗽・呼吸困難を有する症例に対して, 少量のモルヒネとFPの併用は, 安定した症状緩和を得るうえで有用であると考える.

著者

Yoshitake Yamada Shigeo Okuda Masaharu Kataoka Akihiro Tanimoto Yuichi Tamura Takayuki Abe Tomonori Okamura Keiichi Fukuda Toru Satoh Sachio Kuribayashi

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.1204091687, (Released:2012-04-12)

参考文献数

24

被引用文献数

26 33

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Background: Because few have reported the prognostic significance of cardiac magnetic resonance imaging (CMR) for idiopathic pulmonary arterial hypertension (IPAH), in this study we evaluated the value of CMR measurements as a prognostic predictor of IPAH before starting intravenous prostacyclin therapy. Methods and Results: A total of 121 consecutive CMR studies for evaluating right ventricular (RV) function were reviewed. Forty-one patients were diagnosed with IPAH and served as the study group. Factors, such as age, sex, New York Heart Association functional class (NYHAFC), 6-min walk test, plasma brain natriuretic peptide level, serum uric acid level and CMR measurements were analyzed as predictors of first hospitalization and death. The mean follow-up period was 1,350±769 days. Nine patients were hospitalized because of heart failure, and 4 patients died from cardiopulmonary causes. The univariate analyses suggested that the left ventricular (LV) mass index, the left and right ventricular end-diastolic volume indices (LVEDVI, RVEDVI), the LV and RV end-systolic volume indices (LVESVI, RVESVI) and NYHAFC predicted the risk for hospitalization and that RVEDVI, RVESVI and NYHAFC predicted mortality. The multivariate analyses suggested that RVEDVI and NYHAFC are independent predictors of both hospitalization and mortality. The effects of RVEDVI and NYHAFC on hospitalization were not substantially affected by the concomitant medication. Conclusions: In IPAH patients, the RVEDVI predicts both hospitalization for right heart failure and mortality before initiating intravenous prostacyclin therapy.

著者

Masaji MASE Kikuyasu NAKAMURA Fujiko MINAMI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1204110811, (Released:2012-04-20)

被引用文献数

38 51

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Nine fowl adenoviruses (FAdVs) isolated from chickens with inclusion body hepatitis (IBH) in Japan from 2009 to 2010 were characterized serologically and genetically. These isolates were all neutralized by antisera against the SR-48 strain (FAdV-2). Phylogenetic analysis based on the part of the hexon gene that included the L1 region revealed that all isolates were almost identical except one isolate in 2009. This suggests a common ancestor for the FAdVs obtained from chickens with IBH in Japan in 2010.

著者

Elias GEBRU Dereje DAMTE Seung-Jin LEE Joong-Su LEE Young-Hoan KIM Seung-Chun PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1204120812, (Released:2012-04-20)

被引用文献数

10 15

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The in vitro activity of 15 antimicrobial agents against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella spp. and Streptococcus canis from dogs was investigated. For Staphylococcus spp., the highest frequency of resistance was observed for penicillin, followed by ampicillin, tetracycline, and chloramphenicol. The highest frequency of resistance in E. coli isolates was recorded for tetracycline and streptomycin. Pasteurella spp. and S. canis had the highest resistance rate for tetracycline and chloramphenicol. Most isolates showed full susceptibility to low-level resistance to colistin, florfenicol and fluoroquinolones. Further studies using larger number of isolates from both healthy and diseased dogs would provide a broader picture of antimicrobial resistance at a national level and promote prudent use of antimicrobial agents in companion animals.

著者

Atsushi KIMURA Shigeru SATO Takuma KATO Kentaro IKUTA Norio YAMAGISHI Keiji OKADA Hitoshi MIZUGUCHI Kazunori ITO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1204120813, (Released:2012-04-20)

被引用文献数

17 22

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To assess the relationship between pH and temperature in the ruminal bottom fluid, circadian changes were monitored using cows fed a control diet (C diet) or a rumen acidosis-inducing diet (RAI diet) by using a wireless radio-transmission pH- measurement system. These two parameters were measured simultaneously at 10-min intervals on day 14 after commencement of feeding. Compared to the mean ruminal pH for 60 min immediately after the morning feeding (0 hr), significantly lower pH was noted 3–13 hr later (P<0.05) and 4–19 hr later (P<0.01) in cows fed the C and RAI diets, respectively, although the reduction in the latter was much higher than that in the former. In contrast, significantly higher ruminal temperature was found at 8 and 12–14 hr later (P<0.05) and 6, 8, and 10–19 hr later (P<0.01) in cows fed the C and RAI diets, respectively. A significant negative correlation was observed between the lowest ruminal pH and its corresponding ruminal temperature in cows fed the C and RAI diets (r=-0.722 and -0.650, P<0.01, respectively), suggesting active fermentation and volatile fatty acid production in the rumen. However, ruminal pH profiles may not be predictable by measuring only ruminal temperature because decreases in ruminal pH were preceded by increases in ruminal temperature, and circadian changes in pH and temperature were associated with ruminal fermentation.

著者

Naoki HAYASHI Satoru MONZEN Koichi ITO Tsuyoshi FUJIOKA Yukio NAKAMURA Ikuo KASHIWAKURA

出版者

Journal of Radiation Research Editorial Committee

雑誌

Journal of Radiation Research (ISSN:04493060)

巻号頁・発行日

vol.53, no.2, pp.195-201, 2012 (Released:2012-04-17)

参考文献数

28

被引用文献数

8

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The present study aimed to estimate the clonogenic and differentiation potential of induced pluripotent stem (iPS) cells exposed to ionizing radiation. Compared with mouse hematopoietic stem/progenitor cells, iPS cells were less sensitive to radiation. To examine the effect of ionizing radiation on the early differentiation pathway of iPS cells, we assessed embryoid body (EB) formation. Although EB formation was observed at all radiation doses, EB diameter decreased in a radiation dose-dependent manner. At the same time, we analyzed the expression of genes specific to differentiation in the initial iPS cells and cells of EB. The expression of the endoderm marker Afp increased remarkably in cells of EB derived from non-irradiated iPS cells; however, in irradiated cells, this expression significantly decreased in a radiation dose-dependent manner. Further, the expressions of the pluripotent stem cell markers Nanog and Oct-4 and the early mesoderm marker Brachyury significantly decreased. The results of the present study suggest that radiosensitivity with regard to gene expression differs at various stages in the early differentiation pathways of iPS cells that lead to the formation of the 3 germ layers; the sensitivity is the highest in the genes expressed during the differentiation pathways of iPS cells, leading to the formation of the endoderm.

著者

小林 由紀子 赤星 和人 原 行弘 辻内 和人 岡村 陽子

出版者

社団法人 日本リハビリテーション医学会

雑誌

リハビリテーション医学 (ISSN:0034351X)

巻号頁・発行日

vol.42, no.11, pp.778-782, 2005 (Released:2006-09-22)

参考文献数

16

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In the rehabilitation of traumatic brain injury, emotional disorders such as a quick temper, depression and irritability are a critical problem, as well as cognitive dysfunctions such as impairment of attention and memory. In addition, it is rare that they are completely isolated and cognitive dysfunction and emotional disorders tend to have an influence on each other. In other words, the stress caused by cognitive dysfunction induces an aggressive emotional disturbance, and unrest of mood accelerates cognitive dysfunctions such as memory and judgment. Therefore, it is important to consider their emotional states when we start rehabilitation for patients with traumatic brain injury. We had a valuable experience in early rehabilitation of two patients with traumatic brain injury. We had very good results by thinking about the emotional disorder first, and thereafter training for the cognitive dysfunction. Case 1, a young male, was a computer programmer. After suffering a traumatic brain injury, he could not think logically and he had many emotional problems with his mother. Case 2 was a middle-aged housewife. She became depressive and negative towards rehabilitation, because of her memory problems post traumatic brain injury. We administered a rehabilitation regime which attached great importance to treating the emotional disorders affecting these two patients. As a result, in the early phase, we were able to make progress in cognitive rehabilitation as well as improving their emotional problems by our intervention, and they overcame their cognitive dysfunctions and resumed their normal daily lives in a month.

著者

矢吹 淳 小出 和欣 小出 由紀子

出版者

動物臨床医学会

雑誌

動物臨床医学 (ISSN:13446991)

巻号頁・発行日

vol.20, no.1, pp.25-29, 2011-03-20 (Released:2012-04-04)

参考文献数

12

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10歳齢のゴールデン・レトリバーが3日前からの食欲低下,嘔吐および白色の下痢を主訴に来院し,各種検査の結果,総胆管閉塞が疑われた。入院にて3日間の内科的治療を行ったが,改善が認められず,CT検査後に外科的治療を行った。CT検査では胆嚢頸部に結石を認めた。開腹手術にて胆石を摘出し,胆嚢切除を行ったが,大十二指腸乳頭部に炎症ならびに狭窄が認められたため,総胆管ステント留置術を実施した。術後,速やかに黄疸は改善され,術後32日目に内視鏡検査を行い,鏡視下にてステントを抜去した。ステント抜去後の経過も良好で,術後1261日に別疾患で死亡するまで閉塞性黄疸の再発は認められなかった。本症例より,総胆管ステント留置術は,短期的な胆汁流出路の確保が必要な症例に対して有用である可能性が示唆された。

著者

小川 純也 小川 浩子 福山 泰広 長谷川 剛 下江 弘美 平松 佳子 栢森 康司

出版者

動物臨床医学会

雑誌

動物臨床医学 (ISSN:13446991)

巻号頁・発行日

vol.20, no.1, pp.19-23, 2011-03-20 (Released:2012-04-04)

参考文献数

5

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犬前十字靱帯断裂に対して脛骨粗面を前方に転位することによる膝関節の安定ができるTTA手術は,その手術手技が比較的簡単で,そのうえ術後の成績も安定しているため今現在では世界中で行われるようになっている。その一方で本邦ではまだあまり一般的には行われていない。本院では,約7年前,まだこの手術法が確立する前からこの手術を始める機会を得られ,その結果数例においてではあるが術後の合併症を経験し,その発生の原因の究明や予防に努め,今現在では安定した手術結果を納めている。今回は,本院における全ての手術成績と合併症を示しその原因と対応を紹介する事で,今後日本においてこの手術を始める時の礎となることを期待している。

著者

中道 潤 安川 邦美 田端 克俊 森下 啓太郎 福井 健太 植野 孝志 下田 哲也

出版者

動物臨床医学会

雑誌

動物臨床医学 (ISSN:13446991)

巻号頁・発行日

vol.20, no.1, pp.7-11, 2011-03-20 (Released:2012-04-04)

参考文献数

6

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動脈管開存症(PDA)による重度の心不全の犬において,貧血と血小板減少症および脾臓の腫瘤病変が認められた。内科治療により心不全の改善を図った後,脾臓摘出術を実施した。術後一旦全身状態は改善したが,貧血,血小板減少症,低アルブミン血症,肝不全,腎不全が進行し死亡した。病理組織学的検査により血球貪食性組織球肉腫と診断した。本症例の貧血および血小板減少症の原因は,脾臓の腫瘍組織における細血管障害性溶血性貧血と腫瘍細胞の血球貪食によるものとが考えられたが,脾臓摘出後も貧血および血小板減少症が進行したことから,腫瘍細胞による貪食が血球減少の主な原因であると考えられた。

著者

岩泉 正和 大谷 雅人 津田 吉晃 平岡 宏一 宮本 尚子 高橋 誠 津村 義彦

出版者

日本森林学会

雑誌

日本森林学会大会発表データベース 第123回日本森林学会大会

巻号頁・発行日

pp.A01, 2011 (Released:2012-02-29)