著者

朝岡 寛史 真名瀬 陽平 野呂 文行 ASAOKA Hiroshi MANASE Youhei NORO Fumiyuki

出版者

障害科学学会

雑誌

障害科学研究 = Japanese Journal of Disability Sciences (ISSN:18815812)

巻号頁・発行日

vol.39, pp.133-140, 2015-03-31

著者

Akihiko Oka Shunji Ishihara Hironobu Mikami Hiroki Sonoyama Tsuyoshi Mishiro Hiroshi Tobita Kousaku Kawashima Tatsuya Miyake Norihisa Ishimura Kenji Furuta Yoshikazu Kinoshita Masayoshi Nishina

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.58, no.17, pp.2485-2494, 2019-09-01 (Released:2019-09-01)

参考文献数

41

被引用文献数

6

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As a rarely recognized foreign body in the upper gastrointestinal tract, rice cake frequently requires endoscopic removal. We herein report six patients with characteristic sonography, computed tomography (CT), spectroscopy, endoscopy, and histological findings. A review of all published cases, including ours, revealed that retained rice cake in the stomach typically shows the following: abdominal pain (93.3%), mucosal injury (93.3%) with bleeding (42.9%); high-density (120-206 Hounsfield units) CT findings; and indication for endoscopy (80%). In the esophagus, hot, toasted rice cake causes thermal injury. Primary physicians should be aware of this popular-food-induced, but rare, disorder.

著者

Matsuura Hiroshi Yazaki Ikuko Okino Tatsufumi

出版者

Springer Japan

雑誌

Fisheries Science (ISSN:09199268)

巻号頁・発行日

vol.75, no.3, pp.777-783, 2009-05

被引用文献数

25

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Larval metamorphosis inducers of the sea cucumber Apostichopus japonicus were screened from physiologically active compounds. Doliolaria larvae completed their metamorphosis to juveniles in 120 hours when treated with 5-10 μM of dopamine and L-DOPA, and 50 μM of L-adrenaline and L-noradrenaline. Doliolaria larvae had to be exposed to dopamine or L-DOPA for at least 24 h. D1-like dopamine receptor antagonists SKF87566 and LE300 (10 μM) inhibited metamorphosis by dopamine. However, the D2-like dopamine receptor antagonists sulpiride and nemonapride (10 μM) did not inhibit the effect of dopamine. The results suggest that D1-like dopamine receptors are involved in larval metamorphosis of the sea cucumber A. japonicus.

著者

Nobuyuki Okahashi Shuichi Kawana Junko Iida Hiroshi Shimizu Fumio Matsuda

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.1, pp.A0073, 2019-08-30 (Released:2019-08-30)

参考文献数

15

被引用文献数

1 18

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Isotope labeling measurements using mass spectrometry can provide informative insights on the metabolic systems of various organisms. The detailed identification of carbon positions included in the fragment ions of dicarboxylic and tricarboxylic acids in central carbon metabolism is needed for precise interpretation of the metabolic states. In this study, fragment ions containing the carbon backbone cleavage of dicarboxylic and tricarboxylic in the Krebs cycle were investigated by using gas chromatography (GC)-electron ionization (EI)-MS and GC-EI-MS/MS. The positions of decarboxylation in the dicarboxylic and tricarboxylic acids were successfully identified by analyses using position-specific 13C-labeled standards prepared by in vitro enzymatic reactions. For example, carboxyl groups of C1 and C6 of trimethylsilyl (TMS)- and tert-butyldimethylsilyl (TBDMS)-derivatized malic and citric acids were primarily cleaved by EI. MS/MS analyses were also performed, and fragment ions of TBDMS-citric and α-ketoglutaric acids (αKG) with the loss of two carboxyl groups in collision-induced dissociation (CID) were observed.

著者

Hiroshi Nogami Jun-ichiro Yagi Shin-ya Kitamura Peter Richard Austin

出版者

The Iron and Steel Institute of Japan

雑誌

ISIJ International (ISSN:09151559)

巻号頁・発行日

vol.46, no.12, pp.1759-1766, 2006 (Released:2006-12-21)

参考文献数

25

被引用文献数

35 55

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The iron and steelmaking industry has been receiving social pressure to reduce energy consumption and environmental load as recent increase in the social awareness on environmental and resource problems. The ironmaking system consumes more than a half of overall energy input to the steelwork and its improvement is expected as a countermeasure for such problems. Numerous attempts through improving the blast furnace operation have been made. This paper analyzes material and energy balances of ironmaking system that consists of hot stove, coke oven, CDQ, sintering and blast furnace. The operation statuses of the blast furnace with natural gas injection, metallic charging and top gas recycling that have been obtained by the kinetic-based numerical simulations are applied to this analysis. The results suggested that the metallic charging to blast furnace decreases both energy input and CO2 emission. The natural gas injection operation decreases the CO2 emission from the iron making system while the decrease in the energy input is small. The top gas recycling operation increases the CO2 emission due to the scrubbed CO2 from the recycled top gas.

著者

Kazuo Omori Naoto Katakami Shoya Arakawa Yuichi Yamamoto Hiroyo Ninomiya Mitsuyoshi Takahara Taka-aki Matsuoka Hiroshi Tsugawa Masahiro Furuno Takeshi Bamba Eiichiro Fukusaki Iichiro Shimomura

出版者

Japan Atherosclerosis Society

雑誌

Journal of Atherosclerosis and Thrombosis (ISSN:13403478)

巻号頁・発行日

pp.52506, (Released:2020-01-25)

参考文献数

32

被引用文献数

15

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Aim: An identification of the high-risk group of atherosclerotic cardiovascular disease (CVD) is important in the management of patients with diabetes. Metabolomics is a potential tool for the discovery of new biomarkers. With this background, we aimed to identify metabolites associated with atherosclerosis in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 176 patients with T2DM who have never had a CVD event and 40 who were survivors of coronary artery disease (CAD) events were enrolled. Non-targeted metabolome analysis of fasting plasma samples was performed using gas chromatography coupled with mass spectrometry (GC/MS) highly optimized for multiple measurement of blood samples. First, metabolites were screened by analyzing the association with the established markers of subclinical atherosclerosis (i.e., carotid maximal intima-media thickness (max-IMT) and flow-mediated vasodilation (FMD)) in the non-CVD subjects. Then, the associations between the metabolites detected and the history of CAD were investigated. Result: A total of 65 annotated metabolites were detected. Non-parametric univariate analysis identified inositol and indoxyl sulfate as significantly (p<0.05) associated with both max-IMT and FMD. These metabolites were also significantly associated with CAD. Moreover, inositol remained to be associated with CAD even after adjustments for traditional coronary risk factors. Conclusions: We identified novel biomarker candidates for atherosclerosis in Japanese patients with T2DM using GC/MS-based non-targeted metabolomics.

著者

Manabu NEMOTO Toru KANNO Hiroshi BANNAI Koji TSUJIMURA Takashi YAMANAKA Hiroshi KOKADO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.11, pp.1889-1891, 2017 (Released:2017-11-17)

参考文献数

17

被引用文献数

17

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A vaccine for equine coronavirus (ECoV) is so far unavailable. Bovine coronavirus (BCoV) is antigenically related to ECoV; it is therefore possible that BCoV vaccine will induce antibodies against ECoV in horses. This study investigated antibody response to ECoV in horses inoculated with BCoV vaccine. Virus neutralization tests showed that antibody titers against ECoV increased in all six horses tested at 14 days post inoculation, although the antibody titers were lower against ECoV than against BCoV. This study showed that BCoV vaccine provides horses with antibodies against ECoV to some extent. It is unclear whether antibodies provided by BCoV vaccine are effective against ECoV, and therefore ECoV challenge studies are needed to evaluate efficacy of the vaccine in the future.

著者

大西 浩志 Hiroshi ONISHI

出版者

東京理科大学

雑誌

理大科学フォーラム : 東京理科大学科学教養誌 (ISSN:13461206)

巻号頁・発行日

vol.35, no.3, pp.34-39, 2018-06

著者

Masaaki Minami Hiroshi Takase Mineo Nakamura Toshiaki Makino

出版者

International Research and Cooperation Association for Bio & Socio-Sciences Advancement

雑誌

Drug Discoveries & Therapeutics (ISSN:18817831)

巻号頁・発行日

vol.13, no.6, pp.335-342, 2019-12-31 (Released:2020-01-20)

参考文献数

30

被引用文献数

4

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Foodborne diseases have become a worldwide problem that threatens public health and welfare. Enteropathogenic Escherichia coli (EPEC) is one of major pathogens of moderate to severe diarrhea. The increased prevalence of EPEC strains that produce extended spectrum β-lactamase (ESBL) has deepened the problem. The fruit of Lonicera caerulea var. emphyllocalyx (LCE) has been used as a traditional food preservative and medicine in northern temperate zones such as Hokkaido Island, Japan. In this study, we investigated the antibacterial effect of LCE fruit extract (LCEE) against EPEC. The antibacterial activities of LCEE were examined by bacterial growth, time-kill curve, soft-agar motility, electron microscopy, and 96 well-microplate biofilm assays. We also investigated the bacterial mRNA expression of biofilm-associated genes (fliC, csgA, and fimA) by quantitative real-time PCR assays. LCEE was found to suppress the growth, time-kill curve, and spread of EPEC. It also reduced the biofilm formation in a dose-dependent manner. Morphological analysis using transmission and scanning electron microscopy revealed that LCEE diminished the function of flagella resulting in reduced motility and biofilm formation. The mRNA expression of all three biofilm associated genes was downregulated under LCEE treatment. Extracts of the fruit of LCE inhibit the motility and biofilm formation of EPEC as a result of the inhibition of flagella development and function. We propose LCEE as a therapeutic candidate for the effective therapy of EPEC-associated infectious diseases.

著者

瀬名 秀明 石黒 浩 中島 秀之 松原 仁 Hideaki Sena Hiroshi Ishiguro Hideyuki Nakashima Hitoshi Matsubara

雑誌

人工知能学会誌 = Journal of Japanese Society for Artificial Intelligence (ISSN:09128085)

巻号頁・発行日

vol.26, no.1, pp.55-62, 2011-01-01

著者

Yoshifumi Saisho Kinsei Kou Kumiko Tanaka Takayuki Abe Hideaki Kurosawa Akira Shimada Shu Meguro Toshihide Kawai Hiroshi Itoh

出版者

The Japan Endocrine Society

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

vol.58, no.4, pp.315-322, 2011 (Released:2011-04-29)

参考文献数

17

被引用文献数

46 72

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Type 2 diabetes is a progressive disease and most patients with type 2 diabetes eventually need insulin therapy. The objective of this study was to clarify C-peptide immunoreactivity (CPR), a marker of beta cell function, as a predictor of requirement for insulin therapy. We conducted a retrospective study of 579 consecutive subjects with type 2 diabetes who were admitted to our hospital from 2000 to 2007 and were able to be followed up for at least 6 months after discharge. Fasting and postprandial serum CPR and urinary CPR levels had been measured during admission. Information about insulin therapy at the last visit was obtained from medical records. At the last visit, 364 subjects (62.9%) were treated with insulin. Mean interval between discharge and the last visit was 4.5 ± 2.3 years. Serum and urine CPR levels at baseline were significantly associated with insulin treatment at the last visit (P

著者

Sato Hiroshi 佐藤 暢

巻号頁・発行日

1998

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Thesis (Ph. D. in Science)--University of Tsukuba, (A), no. 1863, 1998.3.23

著者

Takuo Kubota Masanori Adachi Taichi Kitaoka Kosei Hasegawa Yasuhisa Ohata Makoto Fujiwara Toshimi Michigami Hiroshi Mochizuki Keiichi Ozono

出版者

The Japanese Society for Pediatric Endocrinology

雑誌

Clinical Pediatric Endocrinology (ISSN:09185739)

巻号頁・発行日

vol.29, no.1, pp.25-42, 2020 (Released:2020-01-09)

参考文献数

79

被引用文献数

19

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Achondroplasia (ACH) is a skeletal dysplasia that presents with limb shortening, short stature, and characteristic facial configuration. ACH is caused by mutations of the FGFR3 gene, leading to constantly activated FGFR3 and activation of its downstream intracellular signaling pathway. This results in the suppression of chondrocyte differentiation and proliferation, which in turn impairs endochondral ossification and causes short-limb short stature. ACH also causes characteristic clinical symptoms, including foramen magnum narrowing, ventricular enlargement, sleep apnea, upper airway stenosis, otitis media, a narrow thorax, spinal canal stenosis, spinal kyphosis, and deformities of the lower extremities. Although outside Japan, papers on health supervision are available, they are based on reports and questionnaire survey results. Considering the scarcity of high levels of evidence and clinical guidelines for patients with ACH, clinical practical guidelines have been developed to assist both healthcare professionals and patients in making appropriate decisions in specific clinical situations. Eleven clinical questions were established and a systematic literature search was conducted using PubMed/MEDLINE. Evidence-based recommendations were developed, and the guidelines describe the recommendations related to the clinical management of ACH. We anticipate that these clinical practice guidelines for ACH will be useful for healthcare professionals and patients alike.

著者

Hiroyuki Kajiura Kyoko Hiwasa-Tanase Hiroshi Ezura Kazuhito Fujiyama

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.35, no.4, pp.375-379, 2018-12-25 (Released:2018-12-31)

参考文献数

17

被引用文献数

1 4

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Miraculin is a promising protein with taste-modifying properties. Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation. For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized. Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin. Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope. These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.

著者

渡辺 洋 WATANABE Hiroshi

出版者

岩手大学人文社会科学部

雑誌

歴史と文化

巻号頁・発行日

pp.191-204, 1981-02-20

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行動主義の文学といえば、日本では昭和九年から十年にかけての一時期、衆目を集めながら短命に終った一種の文学運動として理解されている。実際、「行動主義文学」は華々しい論争を展開したわりに実体に乏しく、とりわけ実作化の面で見るべき作品が少なかったことは事実である。しかし、短期間であったとはいえ当時の文学界に投じた波紋は大きく、その主張、理論、活動を無視することもできない。元来「行動主義」は「大戦後佛蘭西の思想、文学の主流をなしていた懐疑・不安・否定の傾向に反発して抬頭したものである」という。戦争という悲劇がヨーロッパ全土を非人間的世界の集合体に一変させ、社会に対する、否、人間存在に対する不安を人々の心に植えつけたことはたしかである。そしてこの暗い影は文学の世界にも当然波及していった。名目だけの戦勝国フランスではその傾向が一層顕著であった。フランスは戦後、経済の面で驚異的な繁栄を謡歌したが、それは束の間の出来事にすぎず、文学の世界はぬぐいきれぬ不安と絶望の雲に厚く覆われていた。こうした社会環境を背景に生まれたのが「行動主義文学」であるといわれている。さらに厳密にいえば、一九二七年ラモン・フェルナンデスによって提唱された「行動的ヒューマニズム」(Humanisme de l'action)に端を発したもので、必らずしも体系的にまとまった文学理論、あるいは運動ではなかった。換言するなら、当時フランスで発表された文学作品に共通して認められた思想、様式、文体などの総称であり、従来のダダイズムや超現実主義の絶望的、懐疑的傾向を否定し、意識的に不安や絶望の克服を目標に掲げた積極的なひとつの姿勢であるといえる。つまり、人間の価値の権威と実存を回復しようとする人間性把趣に関する新しい試みであった。フェルナンデスは、「個的人間をその全体性と独自の現実の上に見るためにはただ行動的角度においてのみ可能である」と主張した。これは時期的にいって絶好の提言であった。実際、フランス文学史上に、今日なおその名をとどめている多くの著名な作家たちによってこの主張は支持されたのである。これをいわゆる「行動主義」として日本に紹介したのが、長年フランスに滞在し昭和六年、帰国した小松清であった。ちょうど文学者の「能動精神」が盛んに叫ばれ出した時でもあり、「行動主義」は日本の文学界に少なからぬ反響を巻き起した。しかし、それほどまでに騒がれ論議されながら、なぜ日本において「行動主義」が短命で終り、実作化に成功しなかったのか。この小論では行動主義の文学といわれている実際の作品を通してその点について考察し、同時にフランスと日本の「行動主義」の文学を比較検討してみたい。

著者

Hiroshi Kuwahata Takeshi Yamaguchi

出版者

The Japan Society of Vacuum and Surface Science

雑誌

e-Journal of Surface Science and Nanotechnology (ISSN:13480391)

巻号頁・発行日

vol.14, pp.231-236, 2016-12-24 (Released:2016-12-24)

参考文献数

25

被引用文献数

1 3

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An atmospheric-pressure argon (Ar) plasma jet was generated with a frequency of 10 kHz, an applied voltage of 10 kV, and an Ar gas flow rate of 10 L/min. When an aluminum (Al) thin film deposited on a glass substrate was irradiated with the Ar plasma jet in air for 5 s, Al was removed. The removal rate was ∼700 nm/s. Because the streamers in the plasma reached the Al thin film, Al was removed and a mark was formed on the film, which was referred to as a removal mark. The shape of the removal mark depended on the irradiation distance: it was ring-shaped with an inner diameter of ∼5 mm and an outer diameter of ∼6 mm for irradiation distances of 2–7 mm, and disc-shaped with a diameter of ∼5 mm for irradiation distances of 10–20 mm. The dependence of the shape of the removal mark on the irradiation distance suggests that the distribution of the streamers in the plasma depended on the irradiation distance. The streamers inside the quartz tube were concentrated along the inner wall. In contrast, the streamers outside the tube were distributed in a ring shape with an inner diameter of ∼5 mm and an outer diameter of ∼6 mm for irradiation distances of 2–7 mm, but they converged then diverged to form a smaller disc shape with a diameter of ∼5 mm for irradiation distances of 10–20 mm. [DOI: 10.1380/ejssnt.2016.231]

著者

Hiroshi Ueda

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.42, no.11, pp.1773-1782, 2019-11-01 (Released:2019-11-01)

参考文献数

67

被引用文献数

4 10

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Currently, only a few medicines have been approved for use in the clinical treatment of chronic pain, but they are not fully satisfying due to their side effects. From the view that radical treatment, rather than simply treating symptoms, is more important in addressing life-long chronic pain, we have been investigating translational research for a mechanism-based medicine to treat pain. Through the characterization of various types of peripheral and central neuropathic pain in mice, we discovered that lysophosphatidic acid (LPA) plays roles in definitive mechanisms of the development and maintenance of neuropathic pain. We found LPA1 receptor- and LPA3 receptor-mediated amplification of LPA production could be a key mechanism underlying the initiation and maintenance of this pain. We have developed stress-induced fibromyalgia models, and have revealed that LPA1 receptor-signaling also plays key roles in the mechanism. Throughout these studies, we found that LPA plays a key role in pain memory, and that LPA1 receptor- and LPA3 receptor-antagonists could reverse the established pain, and thereby cure the disease source of pain.

著者

Ryo Nakabayashi Tomoko Nishizawa Tetsuya Mori Hiroshi Sudo Isao Fujii Takashi Asano Kazuki Saito

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.19.1002a, (Released:2019-12-18)

参考文献数

6

被引用文献数

8

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Asparaptine, a conjugate of L-arginine and asparagusic acid, was found in green asparagus (Asparagus officinalis) using ultrahigh-resolution metabolomics for sulfur-containing metabolites (S-metabolites), called S-omics. Asparaptine has been shown to inhibit the activity of angiotensin-converting enzyme. Larger amounts of this S-metabolite are therefore required for further analysis; however, there are limitations that asparagus is a perennial plant and its spears, wherein asparaptine accumulates, can be mainly harvested at the spring to summer season. In order to overcome these, we prepared a callus and suspension cell line from green asparagus. Untargeted metabolome analysis using liquid chromatography-tandem mass spectrometry was performed in the materials as well as spears and three calluses derived from wild type Asparagus. The analysis demonstrated that the amount of asparaptine in the callus derived from the green asparagus was more than the others per mg dry weight. The suspension cell line treated with methyljasmonate showed the induction of asparaptine, suggesting that the asparaptine production is modifiable under appropriate culture conditions. The described materials can be utilized for the production of asparaptine and in integrated metabolomics to study the biosynthesis of this S-metabolite, which is currently unknown.

著者

Kanako Sekimoto Motoshi Sakakura Hiroshi Hike Takatomo Kawamukai Teruhisa Shiota Mitsuo Takayama

出版者

The Mass Spectrometry Society of Japan

雑誌

Mass Spectrometry (ISSN:2187137X)

巻号頁・発行日

vol.8, no.1, pp.A0075, 2019-10-25 (Released:2019-10-25)

参考文献数

36

被引用文献数

1

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Herein, a dark-current discharge state created by combining argon flow with a needle electrode in ambient air is described that has an ionization efficiency and mechanism comparable to those of conventional helium direct analysis in real time (DART), without requiring dopants and DART glow discharge. Using this method, polar compounds such as α-amino acids were ionized in the dark-current argon discharge via (de)protonation, molecular anion formation, fragmentation, (de)protonation with the attachment of oxygen, deprotonation with hydrogen loss and negative ion attachment. In contrast, nonpolar compounds (e.g., n-alkanes) were detected as positive ions via hydride abstraction and oxidation. Major background ions observed were H3O+(H2O)n, O2·+, O2·−(H2O)n and CO3·−. These results indicate that the present dark-current discharge efficiently generates resonance-state argon with an internal energy of ∼14.2 eV, higher than that of the well-known metastable state (∼11.6 eV). It is therefore suggested that ionization reactions occurring there can be attributed to the Penning ionization of atmospheric H2O and O2 by resonance-state argon, analogous to helium DART.

著者

村井 寛志 Murai Hiroshi 方 志恒 崔 喜植 五野井 郁夫 Gonoi Ikuo 後田多 敦 Shiitada Atsushi 林 泉忠

出版者

神奈川大学アジア研究センター

雑誌

神奈川大学アジア・レビュー (ISSN:21885184)

巻号頁・発行日

vol.05, pp.76-98, 2018-03-20

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