著者

Kouko HAMAMOTO Yasuharu MIZUNO Masaki KATO Norio YAMAGISHI Kazuhisa FURUHAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0220, (Released:2012-11-13)

被引用文献数

3 4

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A quantitative assay method by LC/ESI-MS/MS for the simultaneous determination of MCP in cattle plasma was developed and validated. The chromatographic separation was carried out using a multimode column (2 mm × 150 mm, 3 μm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). MCP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and m/z 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5–500 ng/ml (R2>0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96–103% and a coefficient of variation was less than 6.5%. Plasma MCP concentrations after 0.4 mg/kg intravenous administration to 12 cattle were determined by the LC-MS/MS method.

著者

Adrian Patalinghug YBAÑEZ Thillaiampalam SIVAKUMAR Badgar BATTSETSEG Banzragch BATTUR Khukhuu ALTANGEREL Kotaro MATSUMOTO Naoaki YOKOYAMA Hisashi INOKUMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0361, (Released:2012-11-13)

被引用文献数

4 37

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Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA fragments of an Anaplasma sp. closely related to A. ovis (with 95.0% identity) were detected using a different nPCR method based on groEL gene. The phylogenetic analyses based on the Msp5, groEL and 16S rRNA genes demonstrated that A. marginale isolates in Mongolia were not divergent from the isolates distributed in other countries. The present study successfully established a new nPCR assay that can detect A. marginale, and reported the first molecular detection and characterization of A. marginale and an Anaplasma sp. closely related to A. ovis in Mongolian cattle populations.

著者

Takami Kakuda

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.25, no.12, pp.1513-1518, 2002 (Released:2002-12-01)

参考文献数

61

被引用文献数

65 121 133

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The neuroprotective effects of theanine and catechins contained in green tea are discussed. Although the death of cultured rat cortical neurons was induced by the application of glutamic acid, this neuronal death was suppressed with exposure to theanine. The death of hippocampal CA1 pyramidal neurons caused by transient forebrain ischemia in the gerbil was inhibited with the ventricular preadministration of theanine. The neuronal death of the hippocampal CA3 region by kainate was also prevented by the administration of theanine. Theanine has a higher binding capacity for the AMPA/kainate receptors than for NMDA receptors, although the binding capacity in all cases is markedly less than that of glutamic acid. The results of the present study suggest that the mechanism of the neuroprotective effect of theanine is related not only to the glutamate receptor but also to other mechanisms such as the glutamate transporter, although further studies are needed. One of the onset mechanisms for arteriosclerosis, a major factor in ischemic cerebrovascular disease, is probably the oxidative alteration of low-density lipoprotein (LDL) by active oxygen species. The oxidative alterations of LDL were shown to be prevented by tea catechins. Scavenging of ·O2− was also exhibited by tea catechins. The neuroprotective effects of theanine and catechins contained in green tea are a focus of considerable attention, and further studies are warranted.

著者

Yuji Hirai Toshio Yoshihara Toshiko Motoji Kyoichi Totsuka

出版者

一般社団法人 日本内科学会

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.51, no.20, pp.2989-2990, 2012 (Released:2012-10-15)

参考文献数

2

著者

松崎 達 中澤 敦 大塚 征爾 前田 憲男 米井 嘉一 稲垣 恭孝 鈴木 修 桐生 恭好 水野 嘉夫 小川 健二

出版者

一般財団法人 日本消化器病学会

雑誌

日本消化器病学会雑誌 (ISSN:04466586)

巻号頁・発行日

vol.98, no.3, pp.300-306, 2001 (Released:2008-02-26)

参考文献数

20

被引用文献数

3 3

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症例は47歳男性,直腸炎型の潰瘍性大腸炎(以下UC)で通院中に突然,右下肢腫脹・疼痛を認めて入院,ドプラエコーで下大静脈から膝窩静脈におよぶ広汎な静脈血栓症と診断.本邦のUC患者ではこれまで18例の深部静脈血栓症合併例が報告されているが,検索しえた限りでは直腸炎型での合併は初めてであった.抗凝固療法と血栓溶解療法の併用により深部静脈血栓症にともなう症状のみならず,UCの臨床症状の改善を認めた.

著者

西岡 淑雄

出版者

日本英学史学会

雑誌

英学史研究 (ISSN:03869490)

巻号頁・発行日

vol.1993, no.25, pp.87-101, 1992 (Released:2009-10-07)

参考文献数

20

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Steamship lines between San Francisco and Yokohama were opened in 1867 by the Pacific Mail Steamship Company, the Colorado being the first steamer that arrived in Yokohama.The steamers on this line were the Colorado, the Great Republic, the America, the Japan, and the China. They were wooden paddle steamers, among which the America was destroyed by the fire on Aug. 24, 1872 at Yokohama.Later, the City of Tokio and the City of Peking, iron ships with propellers, were added to the line up.A large number of passengers Japanese as well as foreign crossed the Pacific. Those who went over to America and Europe using this line for the purpose of studying abroad contributed to the civilization of a new Japan.We can find the list of passengers in the columns of English newspapers But their names are often misspelt by the editors of the paper. So it is sometimes puzzling who the names refer to.

著者

Hirohito OGAWA Rajesh CHAHOTA Kenji OHYA Tsuyoshi YAMAGUCHI Hideto FUKUSHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0367, (Released:2012-11-12)

被引用文献数

2 7

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Beak and feather disease virus (BFDV) is a causative agent of psittacine beak and feather disease (PBFD), which shows a characteristic feather disorder in psittacine birds. In the present study, the subclinical infection rate of PBFD in imported and domestically bred psittacine birds was investigated by polymerase chain reaction. As a result, 126 of 402 birds (31.3 %) were found to be BFDV positive. The DNA sequences of the part of open reading frame (ORF) C1 were determined for 16 BFDV-positive randomly selected samples. One of 16 samples was found to have a mixed infection, and 5 different BFDV sequences were obtained from a single African grey parrot. In phylogenic analysis, almost BFDV sequences included in each genetic cluster of phylogenic tree belonged to the same psittacine subfamily. BFDV derived from African grey parrot was closely related to the BFDV derived from cockatoos by way of exception. The natural habitat of the African grey parrot and cockatoos is different, therefore the possibility of interspecies cross infection through the bird trade is suggested from the exceptional BFDV sequences.

著者

伊藤 信輔 井上 望 平野 実

出版者

一般社団法人 日本めまい平衡医学会

雑誌

Equilibrium Research (ISSN:03855716)

巻号頁・発行日

vol.45, no.3, pp.281-283, 1986 (Released:2009-06-05)

参考文献数

7

被引用文献数

1

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Slow eye movement induced by a vestibulo-ocular reflex was enhanced or inhibited in various postures of rabbits. A tonic neck reflex functions to help pursuit eye movement in both active and passive postures.

著者

Qing LI Yong FAN Xiaofang SUN Yanhong YU

出版者

日本繁殖生物学会

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

pp.2012-109, (Released:2012-11-09)

被引用文献数

2 13

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The ectopic expression of transcription factors for reprogramming human somatic cells to a pluripotent state represents a valuable resource for the development of in vitro-based models for human disease and has great potential in regenerative therapies. However, the majority of studies have used skin fibroblasts to generate induced pluripotent stem cells (iPSCs) that typically require the enforced expression of several transcription factors, thereby posing a mutagenesis risk by the insertion of viral transgenes. To reduce this risk, iPSCs have been generated with OCT4 and KLF4 from human neural stem cells that endogenously express the remaining reprogramming factors. However, human neural stem cells are rare and difficult to obtain. Here, we show that iPSCs can be generated from human amniotic fluid cells (hAFCs) with two transcription factors: OCT4 and KLF4. Furthermore, iPSCs can be readily derived from hAFCs in a feeder-free conditions, thereby eliminating the potential variability caused by using feeder cells. Our results indicate that hAFCs represent an accessible source of cells that can be reprogrammed into pluripotent stem cells with two Yamanaka factors. Therefore, hAFCs may become a preferred cell type in the future for safe reprogramming without any exogenous genetic material.

著者

古本 英晴

出版者

日本認知科学会

雑誌

認知科学 (ISSN:13417924)

巻号頁・発行日

vol.7, no.3, pp.210-222, 2000-09-01 (Released:2008-10-03)

参考文献数

49

被引用文献数

1

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Although many cases have been reported on impairment of tool use and behavioral disturbance due to brain damage, the precise brain mechanisms regulating human behavior are still unknown.In this report, an outline of behavioral abnormalities caused by brain damage, including utilization behavior (UB), imitation behavior (IB), and apraxia are presented. Next, a case of UB and IB elicited only by a key person is reported, and interpretation of his symptoms is discussed. Lastly, the general structure of information processing in the human brain for tool use and behavior are discussed. I speculate that there are two distinct systems in the human brain, that is, a control system and a substantial information processing system. The former corresponds to attention and emotion. This distinction should have importance when considering tool use and behavior in human beings.A right-handed, 70 year-old man suffered bilateral anterior cerebral artery occlusion that resulted in bilateral frontal lobe infarction. He was inert, and his attention was impaired. He showed typical UB and IB which were elicited only by his chief physician and only in examination situations. Both UB and IB were lacking in his daily life and were never elicited by his family members or other medical personnel. Another report also gives evidence of cases in which a key person is required for UB and/or IB to be elicited.Generally, an object could be considered to always show its meaning as a tool, which implies that its affordace emerges from the object itself. The behavior presented by the key person also can be regarded as a releaser for the patient to imitate behavior. Thus his essential impairment can be attributed to failure to grasp the correct relation of the meaning of objects or of behaviors and situations. This should be called a type of semantic impairments.On the other hand, the patient's inertia and impaired attention is indicative of emotional disturbance. In fact, his cerebral lesions involve some parts of the limbic system. This suggests that he lost active perception which resulted in his being very easily captured by external stimuli. His unique symptoms should not be attributed to the traditional explanation in which UB and/or IB emerge from simple disinhibition of the parietal lobe function from the frontal lobe function.

著者

吉田 信行

出版者

公益社団法人 日本畜産学会

雑誌

日本畜産学会報 (ISSN:1346907X)

巻号頁・発行日

vol.8, pp.305-322, 1935 (Released:2008-03-10)

参考文献数

47

著者

Tomoshige BABA Atsushi SHIMIZU Tamio OHMURO Naohiro UCHIDA Kumiko SHIBATA Masahiko NAGATA Kinji SHIROTA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.71, no.1, pp.79-82, 2009 (Released:2009-02-05)

参考文献数

12

被引用文献数

2 8 2

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An adult, Japanese domestic cat presented with bilateral swelling, distortion, and erythema of ears and deformation of the limbs. Biopsy of the pinnae confirmed auricular chondritis. These lesions and the cat's general condition subsequently deteriorated, and the cat died. At necropsy, chondral changes were present in the pinnae, costae, larynx, trachea, and limbs. Histopathologically, these chondral tissues showed marked deformation with lymphocytic inflammation. The limb joint inflammation was associated with chondral erosion, deformation of subchondral bones with pannus, and thinning of cancellous bones. These lesions were consistent with the diagnostic criteria for human relapsing polychondritis. However, the cat had erosive arthritis, which appeared to be different from human relapsing polychondritis.

著者

高橋 行雄

出版者

公益社団法人 日本薬理学会

雑誌

日本薬理学雑誌 (ISSN:00155691)

巻号頁・発行日

vol.133, no.6, pp.325-331, 2009 (Released:2009-06-12)

参考文献数

11

被引用文献数

3

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薬理学研究から得られる経時データに対して,様々な統計手法が適用されている.しかし,適切な統計解析法なのかが明確にされていないために,反復測定分散分析(Repeated measures ANOVA)を行い,薬剤因子と時間因子の交互作用に有意な差があれば,時点ごとに多重比較法により輪切り的に群間の平均値を比較する方法が標準的になっている.今回,経時的に測定された血圧データを用いて,降圧剤の薬効評価の観点からその問題点について検討した.反復測定分散分析において,投与前値を含む実データを使うか,投与前値からの差のデータを使うかによって分散分析表の見方が全く異なることを示した.経時データのエンドポイントとして,1)効果が最大となる時点,2)曲線下面積,3)薬効の発現時点,4)投与前値までの回復を取り上げ,設定のための着眼点について例示した.投与前値をどのように考慮するかについて,1)投与開始後のデータのみを用いた方がよい場合,2)投与前値を共変量としてよい場合,共変量としてはならない場合に分けて述べた.投与中止後の投与前値への血圧の回復を判定する場合には,有意差検定より差の信頼区間を用いる方が望ましいことを例示した.

著者

村田 厚生 越智 啓太 森若 誠

出版者

一般社団法人 日本人間工学会

雑誌

人間工学 (ISSN:05494974)

巻号頁・発行日

vol.47, no.5, pp.190-197, 2011-10-15 (Released:2011-12-20)

参考文献数

18

被引用文献数

1

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視線データからディスプレイ上の視線位置を表示する手法として, 適応的移動平均法を提案して,移動平均法(10点),注視点直接表示とパフォーマンスを比較し,さらにカーソルを非表示にした場合のパフォーマンスへの影響を明らかにした.コマンド名の表示領域から隣接する選択領域に直接視線が入ることで選択したと見なすI-QGSM(Improved-Quick Glance Selection Method)法をメニュー選択法として用い,メニュー選択作業を被験者に実施させた.作業完了時間,エラー率,作業しやすさ・眼の疲労度の主観評価の観点から,提案した適応的移動平均法が望ましいことが示された.カーソル表示と非表示条件での作業完了時間とエラー率には,ほとんど差がなかった.また,主観評価を通して,カーソルを表示する場合よりカーソルを表示しない場合の方が,操作性の評価が高くなり,眼への疲労度も低いことがわかった.

著者

板谷 正紀

出版者

特定非営利活動法人 日本レーザー医学会

雑誌

日本レーザー医学会誌 (ISSN:02886200)

巻号頁・発行日

vol.28, no.2, pp.146-159, 2007-07-15 (Released:2008-07-16)

参考文献数

31

被引用文献数

2 1

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光干渉断層計(OCT)は,さまざまな眼底疾患の病態の理解を促進した.OCTは,また,網膜厚や網膜神経線維厚を計測することができ,早期緑内障診断,疾患進行のモニター,治療効果の評価に有用である.最近,フーリエドメイン(スペクトラルドメインまたはフリークエンシードメインともいう)検出により撮影速度および撮影感度が,著しく改善した.撮影速度は,標準のタイムドメインOCT(TD-OCT)に比べ40~100倍速い.スペクトラルドメインOCT(SD-OCT)は,網膜病変の3次元解析を可能にする.すなわち,網膜硝子体界面の3次元可視化や網膜内部構造の包括的観察が可能になる.SD-OCTは,網膜神経線維層,網膜内層,または視細胞外節などの網膜層構造をセグメンテーションすることを可能とし,その肥厚や菲薄化をモニターできる.SD-OCTは,ドルーゼン,網膜色素上皮剥離,漿液性網膜剥離のセグメンテーションを可能とし,疾患の進行や治療効果の評価をTD-OCTより精密に行うことが可能となる.SD-OCTは,3次元画像を,眼科診療で使用する眼底カメラや蛍光眼底造影のイメージとレジストレーションすることが可能になる.このように,SD-OCTは,眼底疾患や緑内障の診断における光干渉断層計の潜在力を著しく高めるであろう.

著者

田中 宏暁

出版者

一般社団法人日本体力医学会

雑誌

体力科学 (ISSN:0039906X)

巻号頁・発行日

vol.54, no.1, pp.39-41, 2005 (Released:2006-12-01)

参考文献数

5

被引用文献数

2 1 1

著者

村田 厚生 林 和也 森若 誠

出版者

一般社団法人 日本人間工学会

雑誌

人間工学 (ISSN:05494974)

巻号頁・発行日

vol.47, no.4, pp.127-138, 2011-08-15 (Released:2011-10-05)

参考文献数

13

被引用文献数

1 3

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視線入力インターフェースの研究が行われているが,実用化には,視線入力における優れたスクロール操作方法の提案が必要不可欠になる.スクロール位置とスクロール速度の関係を非線形化したスクロール方法の提案がなされていない,ブラウザでのスクロール領域の位置の影響が明らかにされていないといった課題がある.本研究では,ウェブページでの情報検索を想定した課題を用いて,スクロール方法(スクロールアイコン法,オートスクロール法,オートスクロール改良法)のパフォーマンス,使いやすさの主観評価を比較し,最適なスクロール方法を明らかにした.その結果,スクロール領域でのカーソル位置とスクロール速度の関係を非線形にしたオートスクロール改良法(2次式組み合わせ),オートスクロール改良法(2次式)の作業完了時間が短く,エラー率が低く,操作性の主観評価も高いことが示された.

著者

柴田 均

出版者

公益社団法人 日本農芸化学会

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.77, no.6, pp.573-575, 2003-06-01 (Released:2008-11-21)

参考文献数

9

著者

Hiroyuki TANI Tomoyo NABETANI Kazumi SASAI Eiichiroh BABA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.67, no.4, pp.363-368, 2005 (Released:2005-05-06)

参考文献数

26

被引用文献数

2 3

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The immune responses of hypothyroid dogs to canine thyroglobulin (cTg) were evaluated for the proliferative ability of peripheral blood mononuclear cells (PBMC). PBMC from three hypothyroid dogs with high titers of thyroglobulin autoantibody (TgAA) and 3 clinically normal dogs were cultured with 5, 10, or 20 μg/ml of cTg for 72 hr. The proliferative responses of the cells were determined by the level of incorporated BrdU. The numbers of cells expressing Thy-1, CD4, CD8 and IgG in the PBMC were counted by the immunofluorescence method. Proliferative responses to cTg were observed in the cells from hypothyroid dogs. The number of cells expressing IgG and CD8 in the hypothyroid dogs tended to be high compared with the clinically normal dogs. The CD4+ cells in cultures from hypothyroid dogs increased depending upon the amount of cTg. There was a significant (P<0.05) positive correlation between the number of CD4+ cells and the concentration of cTg in the cultures from hypothyroid dogs. These findings suggest a possible relationship between canine hypothyroidism and cellular immunity. Loss of self tolerance to thyroid antigens in CD4+ T cells may play an important role in the development of canine hypothyroidism.

著者

Nobuyuki FUJIWARA Hideyoshi KAWASAKI Ryotaro YABE Dale J. CHRISTENSEN Michael P. VITEK Takuya MIZUNO Koichi SATO Takashi OHAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0366, (Released:2012-11-05)

被引用文献数

9 18

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Lymphoma is one of the most common malignant tumors in canine. Chemotherapy results in a high rate of remission; however, relapse and clinical drug resistance are usually seen within a year. Protein phosphatase 2A (PP2A) acts as a tumor suppressor and plays a critical role in mammalian cell transformation. Increased protein levels of SET, endogenous PP2A inhibitor, have been reported to correlate with poor prognosis in human leukemia. Here, we test the potential therapeutic role for a SET antagonist in canine lymphoma. We observed SET protein levels increased in multiple canine lymphoma cell lines compared with primary peripheral blood cells. A novel SET antagonist OP449 increased PP2A activity and effectively killed SET high-expressing canine lymphoma cells, but not SET low-expressing cells. Caspase-3 activation and enhanced Annexin V positive staining were observed after OP449 treatment, suggesting apoptotic cell death by OP449. Consistent with this, pan-caspase inhibitor Z-VAD-FMK blocked OP449 induced cell death. These data demonstrated the potential therapeutic application of SET antagonists for canine lymphoma.