著者

Noemi FUSAKI Hiroshi BAN Akiyo NISHIYAMA Koichi SAEKI Mamoru HASEGAWA

出版者

日本学士院

雑誌

Proceedings of the Japan Academy, Series B (ISSN:03862208)

巻号頁・発行日

vol.85, no.8, pp.348-362, 2009 (Released:2009-10-17)

参考文献数

34

被引用文献数

363 1071 613

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Induced pluripotent stem cells (iPSC) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is an efficient solution for generating safe iPSC. Sendai-viral human iPSC expressed pluripotency genes, showed demethylation characteristic of reprogrammed cells. SeV-derived transgenes were decreased during cell division. Moreover, viruses were able to be easily removed by antibody-mediated negative selection utilizing cell surface marker HN that is expressed on SeV-infected cells. Viral-free iPSC differentiated to mature cells of the three embryonic germ layers in vivo and in vitro including beating cardiomyocytes, neurons, bone and pancreatic cells. Our data demonstrated that highly-efficient, non-integrating SeV-based vector system provides a critical solution for reprogramming somatic cells and will accelerate the clinical application.(Communicated by Kumao TOYOSHIMA, M.J.A.)

著者

Shuhei Maeda Yusuke Urabe Kazuto Takemura Tamaki Yasuda Youichi Tanimoto

出版者

(公社)日本気象学会

雑誌

SOLA (ISSN:13496476)

巻号頁・発行日

vol.12, pp.17-21, 2016 (Released:2016-02-11)

参考文献数

19

被引用文献数

2 10

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We investigated features of the atmosphere and ocean to seek a possible candidate that suppressed the growth of the El Niño event in 2014. In the boreal summer-fall season, equatorially antisymmetric sea surface temperature (SST) anomalies with a positive (negative) sign to the north (south) of the equator prevailed in the central and eastern tropical Pacific. In association with the SST anomalies, cumulus convective activity was enhanced in the region of the climatological Intertropical Convergence Zone (ITCZ). Anomalous southerly surface winds flowing across the equator toward the ITCZ induced upward latent heat flux anomalies and lowered SST in the near-equatorial region. These coherent spatial patterns between SST, wind, and latent heat flux anomalies suggested that the wind-evaporation-SST (WES) feedback sustained the suppression of the El Niño growth. A linear baroclinic model experiment indicated that the enhanced convective heating in the ITCZ also contributed to sustain the anomalous surface southerlies across the equator by the intense meridional atmospheric circulation over the equator. These results indicate that the anomalous southerlies across the equator sustained by the WES feedback and intense convective heating in the ITCZ contributed to the suppression of the El Niño growth.

著者

Hiroyuki Niimi Masami Watanabe Hiroaki Serizawa Takato Koba Ikuo Nakamura Masahiro Mii

出版者

日本育種学会

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.65, no.5, pp.396-402, 2015 (Released:2015-12-19)

参考文献数

28

被引用文献数

2 10

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Optimum conditions for obtaining tetraploid were investigated in raphanobrassica, the intergeneric hybrid between radish (Raphanus sativus) and kale (Brassica oleracea var. acephala) by treating in vitro plants with an anti-mitotic agent, amiprophosmethyl (APM). Initially, no tetraploids but hexaploids and octaploids were induced by the treatments. Although the leaves of these polyploids of raphanobrassica showed chlorosis during subcultures in in vitro conditions, the chlorosis could be successfully prevented by the ethylene inhibitors, both AVG and AgNO3. Based on this result, AVG was added into medium used for the culture after the chromosome doubling treatment, which subsequently resulted in increased survival rates of the treated plant materials as well as increased production rates of polyploids including tetraploid. These polyploid plants showed obviously different characters from the original diploid plant. The tetraploid plant had bigger sizes in shoot, flower and leaf, and more number of leaves than the diploid. On the other hand, the hexaploid and octaploid plants had smaller sizes in shoots and leaves, and less number of leaves than the diploid. Concentration of glucosinolates, functional substances of Brassicaceae crops, did not significantly differ between diploid and tetraploid of raphanobrassica, but reduced in hexaploid and octaploid.

著者

Tohru OKADA Tadashi KAMADA Hiroshi TSUJI Jun-etsu MIZOE Masayuki BABA Shingo KATO Shigeru YAMADA Shinji SUGAHARA Shigeo YASUDA Naoyoshi YAMAMOTO Reiko IMAI Azusa HASEGAWA Hiroshi IMADA Hiroki KIYOHARA Kenichi JINGU Makoto SHINOTO Hirohiko TSUJII

出版者

Journal of Radiation Research 編集委員会

雑誌

Journal of Radiation Research (ISSN:04493060)

巻号頁・発行日

vol.51, no.4, pp.355-364, 2010 (Released:2010-07-29)

参考文献数

49

被引用文献数

130

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In June 1994, the world's first clinical center offering carbon ion radiotherapy opened at the National Institute of Radiological Science (NIRS), Japan. Among several types of ion species, carbon ions were chosen for cancer therapy because they were judged to have the most optimal properties in terms of superior physical and biological characteristics. As of March 2010, 5,196 patients have been registered for carbon ion radiotherapy. Clinical results have shown that carbon ion radiotherapy has the potential to provide a sufficient radiation dose to the tumor, while having acceptable morbidity in the surrounding normal tissues. Tumors that appear to respond favorably to carbon ions include locally advanced tumors as well as histologically non-squamous cell tumor types such as adenocarcinoma, adenoid cystic carcinoma, malignant melanoma, hepatoma, and bone/soft tissue sarcoma. By taking advantage of the unique properties of carbon ions, treatment with small fractions within a short treatment period has been successfully carried out for a variety of tumors. This means that carbon ion radiotherapy can offer treatment for larger numbers of patients than is possible with other modalities over the same time period.

著者

Yasuharu Mamiya Miyuki Hiratsuka Masao Murata

出版者

日本線虫学会

雑誌

日本線虫学会誌 (ISSN:09196765)

巻号頁・発行日

vol.35, no.2, pp.63-70, 2005-12-25 (Released:2011-08-11)

参考文献数

8

被引用文献数

3 4 8

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Wood-decay fungi, Pleurotus ostreatus, Lentinula edodes, Neolentinus lepideus, Trichaptum abietinum and Cryptoporus volvatus, were tested for their ability to affect the pinewood nematode population in wood by inoculation experiments. Logs of 1m long and branches of 30cm long, cut from healthy pine trees, were inoculated with P. ostreatus and the pinewood nematode. After inoculation, increases in nematode population in wood were compared with those in logs and branches which were inoculated with nematodes alone. Population in wood inoculated with the fungus was significantly less than that in wood without fungus inoculation. Throughout inoculation experiments of other wooddecay fungi, it was demonstrated that L. edodes, N lepideus and T abietinum also showed their ability to control nematode population in wood. C. volvatus were not recognized for their ability to control the nematode population in wood. Inoculation of P. ostreatus and L. edodes to branches cut from dead pine trees killed by the pinewood nematode did not cause decrease in nematode population in dead wood. Fungi might be unable to grow in dead wood because of lack of food resources, such as parenchyma cells which had been destroyed by the pinewood nematode. Jpn. J. Nematol. 35 (2), 63-70 (2005).

著者

又野 豊 吉光 雅志 後藤 善則

出版者

一般社団法人 日本消化器内視鏡学会

雑誌

日本消化器内視鏡学会雑誌 (ISSN:03871207)

巻号頁・発行日

vol.55, no.8, pp.2228-2229, 2013 (Released:2013-08-28)

参考文献数

4

著者

Hiroko Tabunoki Hidemasa Bono Katsuhiko Ito Takeshi Yokoyama

出版者

バイオ&ソーシャル・サイエンス推進国際研究交流会

雑誌

Drug Discoveries & Therapeutics (ISSN:18817831)

巻号頁・発行日

pp.2016.01011, (Released:2016-02-07)

参考文献数

48

被引用文献数

1 34

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Bombyx mori (silkworm) is the most famous lepidopteran in Japan. B. mori has long been used in the silk industry and also as a model insect for agricultural research. In recent years, B. mori has attracted interest in its potential for use in pathological analysis of model animals. For example, the human macular carotenoid transporter was discovered using information of B. mori carotenoid transporter derived from yellow-cocoon strain. The B. mori carotenoid transport system is useful in human studies. To develop a human disease model, we characterized the human homologs of B. mori, and by constructing KAIKO functional annotation pipeline, and to analyze gene expression profile of a unique B. mori mutant strain using microarray analysis. As a result, we identified a novel molecular network involved in Parkinson's disease. Here we describe the potential use of a spontaneous mutant silkworm strain as a human disease model. We also summarize recent progress in the application of genomic information for annotation of human homologs in B. mori. The B. mori mutant will provide a clue to pathological mechanisms, and the findings will be helpful for the development of therapies and for medical drug discovery.

著者

Takayuki Masuo Masatoshi Ishida Masaki Nishimura Tomoyuki Akai

出版者

公益社団法人 地盤工学会

雑誌

Japanese Geotechnical Society Special Publication (ISSN:21888027)

巻号頁・発行日

vol.2, no.70, pp.2391-2395, 2016-01-31 (Released:2016-01-29)

参考文献数

4

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We have developed gas-permeable/waterproof sheet (GPWS), comprising a micro-porous sheet which is waterproof and have gas permeability, coated with nonwovens to protect and reinforce it, and carried out many experiments in both laboratories and fields. GPWS has been applied as cover sheet for temporary storage sites of decontamination waste due to the Fukushima nuclear accident. On the other hand, geomembrane which is waterproof but gas-impermeable has also employed as cover sheet of the decontamination waste in conjunction with gas-vent pipes. In this study, temperature and oxygen-concentration of the decontaminated wastes, over which GPWS and geomembrane were installed respectively, were experimentally measured in real temporary storage site to compare the influence of weather condition. As the results, it was demonstrated that GPWS can achieve sufficient performances as the cover sheet in that both temperature and oxygen-concentration, closely relating with the consistency of gas permeability in the cover system, was less influenced by weather condition.

著者

寺岡 行雄

出版者

日本森林学会

雑誌

日本森林学会大会発表データベース 第121回日本森林学会大会

巻号頁・発行日

pp.279, 2010 (Released:2010-03-12)

著者

永井 雅人 大平 哲也 安村 誠司 高橋 秀人 結城 美智子 中野 裕紀 章 文 矢部 博興 大津留 前田 正治 高瀬 佳苗 福島県「県民健康調査」グループ

出版者

日本公衆衛生学会

雑誌

日本公衆衛生雑誌 (ISSN:05461766)

巻号頁・発行日

vol.63, no.1, pp.3-10, 2016 (Released:2016-01-29)

参考文献数

24

被引用文献数

2

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目的 東日本大震災による避難者において,生活習慣病が増加していることが報告されている。避難による生活環境の変化に伴い,身体活動量が減少したことが原因の一つとして考えられる。しかしながら,これまで避難状況と運動習慣との関連は検討されていない。そこで,福島県民を対象とした福島県「県民健康調査」より,避難状況と運動習慣の関連を検討した。方法 震災時に原発事故によって避難区域に指定された13市町村に居住していた,平成 7 年 4 月 1 日以前生まれの37,843人を解析対象者とした。避難状況は震災時の居住地(13市町村),避難先(県内避難・県外避難),現在の住居形態(避難所または仮設住宅,借家アパート,親戚宅または持ち家)とした。また,本研究では自記式質問票にて運動を「ほとんど毎日している」または「週に 2~4 回している」と回答した者を「運動習慣あり」と定義した。統計解析は,運動習慣がある者の割合を性・要因別(震災時の居住地,避難先,住居形態)に集計した。また,standard analysis of covariance methods を用いて,年齢,および震災時の居住地,避難先,住居形態を調整した割合も算出した。結果 運動習慣がある者の調整割合は,震災時の居住地別に男性:27.9~46.5%,女性:27.0~43.7%,と男女それぞれ18.6%ポイント,16.7%ポイントの差が観察された。避難先別では,男性で県外(37.7%),女性で県内(32.1%)においてより高かったが,その差は小さく男性:2.2%ポイント,女性:1.8%ポイントであった。住居形態別では,男女ともに借家アパート居住者が最も低く,避難所または仮設住宅居住者が最も高かった(男性:38.9%,女性:36.7%)。避難所または仮設住宅居住者に比し,借家アパート居住者で男性:5.4%ポイント,女性:7.1%ポイント,親戚宅または持ち家居住者で男性:2.0%ポイント,女性:4.2%ポイント,それぞれ低かった。結論 避難区域に指定された13市町村に居住していた者の運動習慣がある者の割合は,震災時の居住地および住居形態によって異なっていた一方,県内避難者と県外避難者との間では同程度であった。とくに借家アパートに居住している者における割合が低く,孤立した人々を対象とした新たな生活習慣病予防対策を立案・実行することが必要である。

著者

石橋 克彦

出版者

公益社団法人 日本地震学会

雑誌

地震 第2輯 (ISSN:00371114)

巻号頁・発行日

vol.30, no.3, pp.369-374, 1977-12-05 (Released:2010-03-11)

参考文献数

26

被引用文献数

3

著者

宍倉 正展 原口 強 宮内 崇裕

出版者

公益社団法人 日本地震学会

雑誌

地震 第2輯 (ISSN:00371114)

巻号頁・発行日

vol.53, no.4, pp.357-372, 2001-06-15 (Released:2010-03-11)

参考文献数

32

被引用文献数

1 8

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Two major historical earthquakes of the 1703 Genroku Kanto Earthquake (M 8.2) and the 1923 Taisho Kanto Earthquake (M 7.9) occurred along the Sagami Trough, where the Philippine Sea Plate subducts beneath the North American Plate. As a result of the coseismic crustai movements associated with these earthquakes and with the repeated characteristic earthquakes of two types (the Genroku-type Earthquake and the Taisho-type Earthquake), Holocene emerged shoreline topography has developed along the southern coast of the Boso Peninsula. We investigated the emerged shoreline topography and beach sediments in the Iwai Lowland with help of a new sampling method using the long geo-slicer to clarify the timing and recurrence interval of the Taisho-type Earthquake. A series of ten narrow emerged beach ridges, which are named the BR-I to BR-X in descending order, are distributed below the highest emerged shore platform, 15.5m high above sea level in the lowland. At least eleven times of coseismic events, probably the Taisho-type Earthquake, can be recognized from the number of the emerged shoreline topography. Based on the 14C ages and sedimentary sequences related to the BR-I-IX, the Taisho-type Earthquake occurred at least three times between 6825-6719 cal yrs BP and 5304-5055 cal yrs BP. After the other three non-dated events succeeded, four events are confirmed at 3300-3100 cal yrs BP, before 2306-2118 cal yrs BP, 1292-1264 cal yrs BP and around 900 cal yrs BP. The lowest BR-X was judged to be emerged during the 1923 event by comparing between the topographic map issued in 1886 and the present one. Analyzing intermittent emergent age of beach ridges, the recurrence interval of the Taisho-type Earthquake is within 990 years, and is 380 years at shortest.

著者

Manabu Hagiwara

出版者

(社)電子情報通信学会

雑誌

IEICE Communications Express (ISSN:21870136)

巻号頁・発行日

vol.5, no.2, pp.49-51, 2016 (Released:2016-02-03)

参考文献数

3

被引用文献数

7

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A short proof for the s-deletion error-correction property of Helberg codes is given.

著者

佐藤 克明

出版者

日本臨床免疫学会

雑誌

日本臨床免疫学会会誌 (ISSN:09114300)

巻号頁・発行日

vol.26, no.4, pp.188-189, 2003-08-31 (Released:2009-02-13)

著者

Ippei Watanabe Kentaro Yamada Akira Aso Okio Suda Takashi Matsumoto Takaaki Yahiro Kamruddin Ahmed Akira Nishizono

出版者

国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.66, no.1, pp.17-21, 2013 (Released:2013-01-22)

参考文献数

23

被引用文献数

4 12

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A mass rabies vaccination of dogs has been conducted annually in Japan over the last 60 years. To assess both current levels of rabies virus-neutralizing antibody (VNA) in dogs and the rationale for current vaccination procedures, we used a rapid fluorescent focus inhibition test to determine VNA levels in 756 dogs that had visited animal hospitals in Japan. We found that 51.1% of the dogs that had received 1 rabies vaccination had protective VNA levels (≥0.5 IU/ml) with a geometric mean of 0.61 IU/ml. In contrast, 97.8% of the dogs that had been vaccinated at least twice had protective VNA levels with a geometric mean of 7.86 IU/ml. Furthermore, 97.9–100% of the dogs vaccinated at least twice retained protective VNA levels into the second year after the last vaccination. Although VNA levels in the dogs vaccinated at least twice tended to decline 2 years after the last vaccination, 78.9% retained protective VNA levels. Thus, the current rabies vaccination schedule provides adequate protection, but the registration system and vaccination schedule needs to be improved to ensure that increased numbers of dogs are vaccinated against rabies.

著者

後藤 聖太 斎藤 恭一

出版者

公益社団法人 日本アイソトープ協会

雑誌

RADIOISOTOPES (ISSN:00338303)

巻号頁・発行日

vol.65, no.1, pp.7-14, 2016-01-15 (Released:2016-01-22)

参考文献数

11

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東京電力福島第一原子力発電所の1~4号機取水口前の海水エリアに,原子炉建屋などから漏出した汚染水の一部が流れ込んで16万トンの海水が汚染されている。この汚染海水の処理には,吸着材をそのまま投入・浸漬後に回収する方式が「簡便,確実,そして安全」で有効である。港湾内汚染水から放射性セシウムやストロンチウムを除去するために,組み紐に成型した吸着繊維がその海水エリアに試験投入・浸漬されている。本稿では,セシウム除去用の吸着繊維の作製法や性能について解説する。

著者

古川 綾 浅田 美子 森 貴幸 井上 岳 厚田 幸一郎

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.136, no.2, pp.251-258, 2016 (Released:2016-02-01)

参考文献数

12

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The Asheville Project® began in 1996 in Asheville, North Carolina, where community pharmacists, in collaboration with physicians, provide health coaching to patients with lifestyle diseases to accomplish their improved self-management of the disease. The project has now widely expanded across the United States. With periodical coaching by pharmacists, according to the reports of these programs, patients have been reported to show improvements in self-management and laboratory data, including the number of doctor visits, medication adherence and the number of foot examinations. Economically, the total medical costs for this disease have decreased 34% over a 5-year period by complying with the Asheville Project. In implementing this model in Japan, various questions, such as the feasibility for busy pharmacists to expend 30-60 min for meeting individually with patients, effective collaboration between pharmacy and physician, patients' acceptance of support by pharmacists to modify their behavior, etc. had to be answered. Thus, we developed a program entitled, “A Health Coaching Program by Community Pharmacists in a Collaborative Practice,” aimed at preventing the aggravation of lifestyle diseases; we evaluated its feasibility for the above mentioned concerns. The content of this coaching program has been prepared with reference to the Asheville Project® and with the support of Kitasato University School of Pharmacy and the Iowa Pharmacy Association, USA. We herein introduce this coaching program, as well as what the pharmacists have learned through this program.

著者

川上 憲人 安村 誠司 春日 文子

出版者

公益財団法人 日本学術協力財団

雑誌

学術の動向 (ISSN:13423363)

巻号頁・発行日

vol.19, no.11, pp.11_67-11_67, 2014-11-01 (Released:2015-03-06)

著者

Yusuke KIMURA Ryuji FUKUSHIMA Atsushi HIRAKAWA Masayuki KOBAYASHI Noboru MACHIDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0373, (Released:2016-01-28)

被引用文献数

1 12

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Examination of our necropsy records for the period 2005 to 2014 yielded 41 cases of the endomyocardial form of restrictive cardiomyopathy among 327 cats with evidence of heart disease, and here, we reviewed their epidemiological and clinical features. The medical data obtained retrospectively included signalment, presenting complaints, findings of physical examination, results of various diagnostic tests, methods of treatment and survival times. Except for one Chinchilla Persian cat, all were domestic short-haired cats. The mean age at death was 7.3 ± 4.5 years (median, 6 years; range, 4 months to 19 years), and males accounted for 61% (25/41) of the total. Dyspnea was the most common clinical sign, being evident in 83% (35/41) of the cats. Hind limb paresis or paralysis due to aortic thromboembolism was evident in 41% (17/41). Arrhythmias of atrial origin were common. Echocardiography commonly revealed left atrial or biatrial enlargement with severe endocardial thickening of the left ventricle. Most of the affected cats had a poor outcome; the disease duration ranged from 1 to 977 days, and the median survival period was 30 days.

著者

間 宏

出版者

日本社会学会

雑誌

社会学評論 (ISSN:00215414)

巻号頁・発行日

vol.12, no.1, pp.61-66, 1961-09-30 (Released:2009-11-11)