著者

唐川 正典 胡田 光信 依藤 進 井口 松三

出版者

THE JAPANESE CIRCULATION SOCIETY

雑誌

日本循環器學誌 (ISSN:00471828)

巻号頁・発行日

vol.19, no.7, pp.321-325, 1955-10-20 (Released:2008-04-14)

参考文献数

8

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The authors at first made formula from which assumed normal cardiac size could be calculated in reference to body-weight and length. Actual cardiac size/assumed normal cardiac size was called dilatation-gradient : actual cardiac size was measured by planimeter in its shadow in the direct postero-anterior projection of X-ray. Relatiohship between circulation time and dilatation-gradient was investigated in cases of congestive heart failure and non-congestive cardiac disease, and linear relationship was demonstrated between them. This does not mean that prolongation of real circulation time (decrease of blood velocity) is due to dilatation of heart. Because circulation-time, clinically used, shows not only the blood velocity but also the degree how much the drug injected is diluted by blood.

著者

杉山 尚 鈴木 桂伍

出版者

一般社団法人 日本温泉気候物理医学会

雑誌

日本温泉気候学会雑誌 (ISSN:03694240)

巻号頁・発行日

vol.19, no.4, pp.318-330, 1956-04-25 (Released:2010-08-06)

参考文献数

21

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The authors have studied the effect of the single bathing in the hot spring on the time required for the blood circulation and its relation to the character of the spring and the age, the disease and the blood pressure of the patient, and obtained the following results:1) The circulation time in healthy persons is shortened immediately after the bathing both in the simple carbonate spring (37°C) and in “Unagi-yu” (alkaline sulphur spring, 44°C), but it is prolonged one hour after the bathing, being gradual in the simple carbonate spring bathing (37°C) and rapid in the alkaline spring bathing (44°C).2) As almost similar phenomena are seen in the case of fresh water bathing (37°C and 44°C), they seem to be due to the thermal action of the bathing water.3) Generally speaking, the bathing in the hot spring tends to lower both the maximal and the minimal blood pressures, and the fall keeps on going even one hour after the bathing. It is a characteristic feature of the hot spring bathing that the duration of the fall of the blood pressure is longer in the hot spring bathing than in the fresh water bathing.4) The fall in the blood pressure induced by the bathing in “Unagi-yu” (alkaline sulphur spring) is somewhat irregular and rapid, but that induced by the bathing in the simple carbonate spring is gradual.5) No changes are observed in the pulse after the bathing both in the simple carbonate spring and in the fresh water at 37°C, but a remarkable increase is observed immediately after the bathing in “Unagi-yu” end in the fresh water at 44°C. It returns to the normal pulse after one hour.6) The shortening of the circulation time induced by the hot spring bathing is more remarkably observed in persons with low blood pressure and in younger persons, and the fall in the blood pressure is more remarkable in persons with high blood pressure and in older persons.7) The shortening of the circulation time induced by the hot spring bathing is more remarkable in persons suffering from articular rheumatism than in healthy persons.The return to the normal circulation time is quickest in healthy persons, followed by those suffering from diseases of the stomach, and it is clearly slow in persons suffering from tuberculosis and from rheumatism.

著者

山崎 和博 巻渕 有哉 申 富饒 長谷川 修

出版者

日本神経回路学会

雑誌

日本神経回路学会誌 (ISSN:1340766X)

巻号頁・発行日

vol.17, no.4, pp.187-196, 2010-12-05 (Released:2011-02-07)

参考文献数

19

被引用文献数

1

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自己増殖型ニューラルネットワーク(SOINN)は,追加学習可能なオンライン教師なし学習手法であり,事前にネットワークの構造を決定する必要がないほか,高いノイズ耐性を有し,計算が軽いなどの特長がある.SOINN は特に実世界のデータ処理に有効であり,画像や音声などのパターンの学習·認識や,実環境でオンライン·リアルタイムに稼動する知能ロボットなどに効果的に活用できる.本稿では,SOINN のアルゴリズムを紹介した後,パラメータの調整に関する指針やソフトウェア実装の解説を行なう.

著者

Hye-Sook CHANG Hiroaki KAMISHINA Keijiro MIZUKAMI Yasuyuki MOMOI Masaaki KATAYAMA Mohammad Mahbubur RAHMAN Mohammad Mejbah UDDIN Akira YABUKI Moeko KOHYAMA Osamu YAMATO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.795-798, 2013 (Released:2013-07-01)

参考文献数

9

被引用文献数

9 26

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Canine degenerative myelopathy is an adult-onset, progressive neurodegenerative disease that occurs in multiple dog breeds, particularly Pembroke Welsh Corgis. Recently, a degenerative myelopathy-associated mutation of the canine SOD1 gene was identified as c.118G>A (p.E40K). In the present study, genotyping assays using conventional and real-time PCR methods were developed, and a preliminary genotyping survey was performed on 122 randomly selected Pembroke Welsh Corgis without any degenerative myelopathy-related clinical signs to determine the current allele frequency in Japan. Both of the assays provided clear-cut genotyping. The survey demonstrated the frequencies of the G/G wild-type, G/A heterozygote and A/A homozygote to be 9.0, 42.6 and 48.4%, respectively, indicating that the prevalence of the mutant A allele (69.7%) in Pembroke Welsh Corgis is extremely high in Japan.

著者

Aki FUJIWARA-IGARASHI Yuko GOTO-KOSHINO Hiroyuki MOCHIZUKI Shingo MAEDA Yasuhito FUJINO Koichi OHNO Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.733-742, 2013 (Released:2013-07-01)

参考文献数

45

被引用文献数

6 11

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The p16, p15 and p14 genes are widely known as tumor suppressor genes in human medicine. Although a large number of genetic and epigenetic aberrations in these genes have been reported in human malignancies, canine malignancies have not been well analyzed on the aberrations of these genes. In this study, the full-length complementary DNA (cDNA) of the canine p16 gene was cloned using the 5’ and 3’ rapid amplification of cDNA ends methods. Based on the sequence data, primers specific for p16, p15 and p14 were designed. Using these primers, the expression of p16, p15 and p14 mRNAs could be individually evaluated by reverse transcriptase polymerase chain reaction. Genomic aberrations were also examined using genomic polymerase chain reaction. Two of the 6 canine lymphoid tumor cell lines did not express detectable levels of p16, p15 and p14 mRNAs, and wide-ranging deletions in the p15-p14-p16 genomic locus were suspected. Wide-ranging deletions were also speculated in 2 of 14 dogs with T-cell lymphoid tumors. On the other hand, similar failure of amplification suggesting wide-ranging deletions were not observed in any of the 14 dogs with B-cell lymphoma. Deletion of the p15-p14-p16 genomic locus could be one of the molecular aberrations in canine lymphoid tumor cells.

著者

Katsuhito SONE Hideo AKIYOSHI Junichiro SHIMIZU Zheng CAO Yijyun LI Toshiyuki TANAKA Akiyoshi HAYASHI Shunji SUGII Fumihito OHASHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.685-691, 2013 (Released:2013-07-01)

参考文献数

29

被引用文献数

1 6

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Pulmonary surfactant protein A (SP-A) is used as a biomarker to understand the clinical features of pulmonary diseases and associated prognostic indices in human medicine. This study was conducted to investigate whether or not serum SP-A concentration can be used as a biomarker for identifying pulmonary parenchymal diseases in dogs. Thirty-two dogs with pulmonary parenchymal diseases, 34 with nonrespiratory diseases and 57 healthy dogs were included. Serum SP-A concentration was measured in all dogs using sandwich enzyme linked immunosorbent assay with an anti-dog SP-A polyclonal antibody. Median serum SP-A concentration in healthy dogs was ng/ml, whereas that in dogs with aspiration pneumonia (n=11), primary lung tumors (n=9) and blunt traumatic lung injury (BTLI; n=12) was 3.1, 7.2 and 2.6 ng/ml, respectively; these values were significantly higher than those in healthy dogs. The serum SP-A concentration in dogs with nonrespiratory diseases was comparable with that in healthy dogs. No correlation was observed between the serum SP-A and plasma C-reactive protein concentrations in dogs with aspiration pneumonia and BTLI. There was a significant correlation between the serum SP-A concentration and thoracic radiographic changes in dogs with BTLI. These findings suggest that the serum SP-A concentration may be a useful clinical biomarker of alveolar damage that can be used for differential diagnosis of pulmonary parenchymal diseases and nonrespiratory diseases in dogs.

著者

Sungjin CHOI Jongil LEE Kazuyo IGAWA I-Li LIU Muneki HONNAMI Shigeki SUZUKI Ryohei NISHIMURA Ung-Il CHUNG Nobuo SASAKI Manabu MOCHIZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.721-726, 2013 (Released:2013-07-01)

参考文献数

29

被引用文献数

1 2

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In this study, we aimed to determine the effect of trehalose coating and the optimal dose of basic fibroblast growth factor (bFGF), an osteoinductive protein, loaded onto tailor-made bone implants for implant-induced bone formation in vivo. We fabricated tailor-made α-tricalcium phosphate bone implants (11 mm diameter with 2 parallel cylindrical holes). bFGF 0, 1, 10, 100 or 200 μg/implant was incorporated into implants with and without a trehalose coating, and these were subsequently implanted into dogs to correct temporal bone defects of the same size and shape. Four weeks after implantation, we analyzed the bone implants and surrounding tissues by using micro-computed tomography imaging and histological analyses, as well as gross evaluation. No significant difference in new bone formation was observed between implants with and without a trehalose coating at any of the bFGF doses. Bone implants with 100 and 200 μg bFGF showed significantly more new bone formation at the implant site and within the cylindrical holes of the implants than those without bFGF (Pin vivo, probably due to the presence of blood proteins and electrolytes at the implant site.

著者

Yasushi KATAOKA Chieko ITO Aya KAWASHIMA Miki ISHII Satoko YAMASHIRO Kazuki HARADA Hiroki OCHI Takuo SAWADA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.749-753, 2013 (Released:2013-07-01)

参考文献数

32

被引用文献数

3 14

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The purpose of the present study was to determine the prevalence of antibiotic-resistant enterococci in dogs and cats subjected to differing antibiotic pressures, and the prevalence of vancomycin resistance genes in isolates from these animals. Enterococci were isolated from fecal samples of 65 healthy dogs and 29 healthy cats brought to animal hospitals, from rectal swabs of 73 puppies and 15 kittens from five breeders and two pet shops, and from fecal samples of 20 dogs and 9 cats that were treated with antibiotics in Nippon Veterinary and Life Science University Animal Medical Center. The rates of resistance to ampicillin among isolates from the kitten–puppy group and healthy dog–cat group were 6.8 and 4.3%, respectively. In contrast, the rates of resistance to ampicillin in enterococci from the treatment group under antibiotic pressure were 37.5%. There was a significant difference between the antibiotic-treated group and the untreated group (PPvanA or vanB were not detected in any groups. Therefore, contamination of VRE in dogs and cats is still considered to be minimal in Japan.

著者

Ko KOJIMA Yasuhito FUJINO Yuko GOTO-KOSHINO Koichi OHNO Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.727-731, 2013 (Released:2013-07-01)

参考文献数

21

被引用文献数

4 8

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Lymphoid malignancies, such as leukemia, and many types of lymphoma are common and severe disorders in dogs. Since shortening remission duration caused by resistance to chemotherapy often becomes clinically critical problems, development of novel and effective therapy should be required. The present study investigated the status of NF-κB and effect of its inhibitor, bortezomib, in six canine neoplastic lymphoid cell lines. NF-κB p65 and p50 were detected in the nuclear fraction of GL-1, CLBL-1 and CL-1, suggesting that NF-κB was constitutively activated in the cells. NF-κB p65 was detected in the cytoplasmic fraction of UL-1 and Ema. After incubation with bortezomib, NF-κB p50 and p65 became undetectable in the nuclear fraction of GL-1, CLBL-1 and CL-1, and CLBL-1, respectively, and p65 was clearly degraded in the cytoplasmic fraction of CLBL-1 and CL-1. Bortezomib inhibited the proliferation of all cell lines except Nody-1 in a concentration-dependent manner. The results indicated that constitutive activation of NF-κB could contribute to the proliferation of canine neoplastic lymphoid cells, and bortezomib would have suppressive effects on the NF-κB activation and the proliferation of neoplastic lymphoid cells in dogs.

著者

Ayumi FUJIMOTO Sakurako NEO Chinatsu ISHIZUKA Takashi KATO Kazuhito SEGAWA Shinpei KAWARAI Kikumi OGIHARA Masaharu HISASUE Ryo TSUCHIYA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.6, pp.831-835, 2013 (Released:2013-07-01)

参考文献数

22

被引用文献数

1 7

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The characteristics of surface antigens in canine hepatocellular carcinoma (cHCC) have not been clarified. The objective of this study was to investigate surface antigens, which are considered as stem/progenitor or cancer cell markers, in cHCC cell lines. Expression of various antigens including CD29, CD34, CD44, CD90, CD133 and Dlk-1 was assessed in four cHCC cell lines by flow cytometry. CD44, CD133 and Dlk-1 expression was detectable in all cell lines, and three cell lines expressed CD29. These results indicate that CD29, CD44, CD133 and Dlk-1 have potential as suitable markers in cHCC identification, suggesting that these findings will contribute to the establishment of an early diagnostic tool for the identification of hepatocellular maturation processes.

著者

鏡味 洋史

出版者

日本建築学会

雑誌

日本建築学会技術報告集 (ISSN:13419463)

巻号頁・発行日

vol.13, no.25, pp.347-350, 2007-06-20 (Released:2008-08-01)

参考文献数

9

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The regions of West and Northwest off Hokkaido are situated on the boundary of two major plates of Okhotsk and Eurasian, and an occurrence of repeating earthquake is expected. The author had revealed details of damages due to the Off-Kamuimisaki Earthquaken of 1940 which was the largest one occurred in these regions. Continuing to the previous paper, literature surveys were carried out through documents and newspaper articles to the damaging earthquakes in these regions. damages due to other earthquakes were listed and discussed.

著者

亀田 純 木村 学 O. A. MELINIKOV 早坂 康隆 橋本 善孝 坂島 隆彦 新井 孝志 芳野 極 鈴木 紀毅

出版者

公益社団法人 東京地学協会

雑誌

地学雑誌 (ISSN:0022135X)

巻号頁・発行日

vol.109, no.2, pp.235-248, 2000-04-25 (Released:2010-11-18)

参考文献数

21

被引用文献数

4 8

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The region from Sakhalin in Russia to the eastern margin of the Japan Sea has been regarded as a convergent plate boundary zone between the Eurasia and the North America Plates because large earthquakes and active crustal movements are prominent in this zone. These activities along the eastern margin of the Japan Sea to southern Sakhalin are almost consistent with the expected relative plate motion deduced from the Euler pole which is estimated from the magnetic anomaly lineations in the Atlantic Ocean, but the modern tectonic aspect in northern Sakhalin is inconsistent; e.g., 1995 Neftegorsk Earthquake is one of the typical events.As a result of a structural and tectonic study in northern Sakhalin, the NE-SW compressive tectonic feature since the Late Miocene was clarified. In the northernmost area of the Schmidt Peninsula, the early Cretaceous ophiolite thrusts upon the Late Cretaceous sedimentary rocks. The ophiolite has an overturned sequence: serpentinites, gabbros, basaltic rocks, and hemipelagic sediments in descending order. The Cretaceous sediments form a map-scale and NW vergent synclinorium. All the deformation structures in outcrop scale, such as micro-folds, minor reverse and normal faults, axial plane cleavages and bedding slips, are consistent with a large scale folding. These structural relationships suggest only one event of deformation, which appears to be linked to overthrusting of the ophiolitic rocks.A structural investigation of the southeastern part of the Schmidt Peninsula revealed that the Middle Miocene sediments composed of siltstone and sandstone are folded with wavelength of several tens to several hundred meters. Their fold axes trend in the NW direction, which indicates that deformation under the NE compression is the same as the deformation in the northernmost area. The thickness of sedimentary layers are constant everywhere in folding. This fact indicates that the timing of the deformation is after sedimentation, that is, after the Middle Miocene. This deformation event is consistent with modern activity in northern Sakhalin. Therefore, the modern tectonic framework might have started in the Late Miocene time.A plate tectonic model indicates that the modern relative motion between the Eurasia and the North America Plate started at about 11 m. y. ago. The Late Miocene onset of the modern tectonic framework in northern Sakhalin occurred at almost the same time. The most reliable model to explain the discrepancy of sense of movement from the Eurasia-North America retative motion, may be the “extrusion” of the Okhotsk Block toward the Pacific Ocean. The dextral extrusion boundary in northern Sakhalin may be traced along theeastern coast of Sakhalin to the northern edge of the Kuril Basin, where active seismicityhas been observed although a detailed study of focal mechanisms and other tectonic aspects is needed in the future.

著者

堤 浩之 Andrei I. KOZHURIN Mihail I. STREUTSOV 植木 岳雪 鈴木 康弘 渡辺 満久

出版者

公益社団法人 東京地学協会

雑誌

地学雑誌 (ISSN:0022135X)

巻号頁・発行日

vol.109, no.2, pp.294-301, 2000-04-25 (Released:2010-11-18)

参考文献数

10

被引用文献数

3 6

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We have mapped a series of north-trending faults along the northeastern coast of Sakhalin, Russia, based on aerial photograph interpretation and field observations. These faults include the Upper Pil'tun fault which ruptured during the 1995, Mw 7.0 Neftegorsk earthquake, and the Ekhabi-Pil'tun and Garomay faults east of the surface ruptures. The coseismic displacement associated with the 1995 earthquake shows that the Upper Pil'tun fault is a right-lateral strike-slip fault. Right-lateral stream offsets as much as 80m demonstrate that similar earthquakes have occurred repeatedly along the fault in the late Quaternary. The fault extends for 10 km further to the south beyond the southern termination of the 1995 surface breaks, which is consistent with the coseismic ground deformation detected by SAR interferometry. The Ekhabi-Pil'tun fault trends N10° W to N10°E with a series of fault scarps down-to-the-west across low-relief hilly terrain eroded byperiglacial processes. Right-laterally offset streams and lake shores suggest thatthe Ekhabi-Pil'tun fault is also a right-lateral strike-slip fault. A 3-m-deep trench across the fault north of the Sabo River contains geologic evidence of two episodes of pre -historic surface faulting. The most recent event occurred after 4000 yr B.P. and the penultimate earthquake occurred at about 6200 yr B.P. The interval between the events is greater than 2000 years, which is significantly longer than that obtained for the Upper Pil'tunfault by previous trench excavations.

著者

西尾 正輝 田中 康博 阿部 尚子 島野 敦子 山地 弘子

出版者

日本音声言語医学会

雑誌

音声言語医学 (ISSN:00302813)

巻号頁・発行日

vol.48, no.3, pp.215-224, 2007-07-20 (Released:2010-06-22)

参考文献数

76

被引用文献数

3

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dysarthria263例 (言語治療実施群187例と言語治療を実施しなかった対照群76例) を対象とし, 言語治療成績について検討し以下の結果を得た.1.脳血管障害, 脊髄小脳変性症, パーキンソン病に起因する言語治療実施群では言語治療前後で比較して有意に明瞭度が改善したが, 対照群では有意差は認められなかった.2.脳血管障害に起因する言語治療実施群では, 重症度にかかわりなく有意な明瞭度の改善が認められ, 重症化するほど, 改善の程度が大きくなる傾向が認められた.また, 病期にかかわりなく有意な明瞭度の改善が認められた.3.ALSに起因する言語治療実施群では言語治療前後で比較して有意差は認められなかった.軽度例は経時的に明瞭度がほぼ確実に低下し, 重度例のほとんどは最重度の段階で停滞した.以上の結果に基づいて, dysarthriaの臨床において有効な言語治療手法について検討を加えた.

著者

野中 福次

出版者

公益社団法人 日本アイソトープ協会

雑誌

RADIOISOTOPES (ISSN:00338303)

巻号頁・発行日

vol.4, no.2, pp.37-43, 1956-04-30 (Released:2010-07-21)

参考文献数

12

著者

Tomonori Tatsumi

出版者

理学療法科学学会

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.25, no.5, pp.545-552, 2013-05-25 (Released:2013-06-29)

参考文献数

39

被引用文献数

2 7

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[Purpose] The world of competitive sports has its own unique subculture which at times works towards covering up psychological problems faced by athletes with injuries. The purpose of this study was to develop an “Athletic Injury Psychological Acceptance Scale (AIPAS)” to screen athletes for serious psychological problems resulting from injury. [Subjects] A total of 189 subjects responded to the survey, of which 168 (mean age= 19.93 years; average number of days unable to participate in sports= 71.84 days, SD = 88.01 days) valid responses were subjected to analysis. [Methods] A provisional version of the AIPAS was created from question items based on face-to-face subject interviews and content validity testing by specialists. In order to test criterion-related validity of the AIPAS, subjects were asked to complete indices that would serve as an external criterion. For this purpose, indices that measure athletic rehabilitation dedication and time perspective were designed. [Results] Item analysis of the provisional AIPAS was conducted to confirm the discrimination of each item. Exploratory factor analysis identified “Self-motivation” and “Focus on the Present” as two factors of the provisional scale. Confirmatory factor analysis supported these results. The Cronbach’s alpha was used to measure the internal consistency. Since α=0.81, the reliability of the scale was confirmed. A significant correlation was found between AIPAS and external indices, indicating criterion-related validity. [Conclusion] AIPAS is a reliable and valid scale composed of two subscales.

著者

北原 曜

出版者

公益社団法人 砂防学会

雑誌

砂防学会誌 (ISSN:02868385)

巻号頁・発行日

vol.54, no.5, pp.92-101, 2002-01-15 (Released:2010-04-30)

参考文献数

45

被引用文献数

3

著者

五名 美江 蔵治 光一郎

出版者

日本森林学会

雑誌

日本森林学会誌 (ISSN:13498509)

巻号頁・発行日

vol.94, no.5, pp.214-222, 2012-10-01 (Released:2012-11-22)

参考文献数

13

被引用文献数

1 3

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本研究は東京大学演習林生態水文学研究所穴の宮試験流域を対象とし, 単独流域法を適用して, ハゲ山に砂防植栽を施して森林再生を開始した期間 (前期: 1935∼1946年) と, それから65年が経過して森林に被覆され, 土壌が回復途上にある期間 (後期: 2000∼2011年) で, 森林の洪水緩和機能の指標の一つである降雨量と直接流出量との関係の違いを定量的に明らかにすることを目的とした。その結果, 後期と比べて前期の方が, 同じ降雨量に対して推定直接流出量が大きく, その差は, 200, 300, 400 mmの降雨量の降雨に対してそれぞれ16.0, 25.8, 33.5 mmと推定された。前期と後期の差は, 初期水分条件が乾燥の場合や, 最大降雨強度が大きい場合により明瞭に現れた。例えば, 初期水分条件が乾燥の場合, 後期と比べて前期は, 200, 300, 400 mmの降雨に対して推定直接流出量がそれぞれ19.1, 29.1, 36.6 mm大きく, 最大降雨強度が大きい場合, 後期と比べて前期は, 200, 300, 400 mmの降雨に対して推定直接流出量がそれぞれ36.3, 56.7, 71.3 mm大きかった。一方, 土壌が湿潤な場合および最大降雨強度が小さい場合は, 両者の差は小さかった。

著者

芳野 恭士 岸 由紀乃 金高 隆 古賀 邦正

出版者

公益社団法人 日本栄養・食糧学会

雑誌

日本栄養・食糧学会誌 (ISSN:02873516)

巻号頁・発行日

vol.65, no.5, pp.221-227, 2012 (Released:2012-10-26)

参考文献数

28

被引用文献数

1

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サラシア属植物の一種であるSalacia reticulataの葉と幹の水抽出物について,そのマウスI型アレルギー抑制作用を検討した。S. reticulataの葉および幹の水抽出物を,200もしくは400 mg/kg体重の投与量でマウスに経口投与した場合,マウスI型アレルギーを有意に抑制した。I型アレルギーマウスにおける腹壁中のインターリューキン-4やインターリューキン-10,さらには免疫グロブリンEの濃度の上昇もまた,これらの抽出物の投与により抑制された。S. reticulataの葉水抽出物中の有効成分の一部として(-)-エピカテキン,また,その幹水抽出物中の有効成分として,マンギフェリンが考えられる。したがって,S. reticulataの葉と幹は,I型アレルギーを予防するのに適した食品素材であると考えられる。

著者

Chitwadee PHITHAKROTCHANAKOON Verawat CHAMPREDA Sei-ichi AIBA Kusol POOTANAKIT Sutipa TANAPONGPIPAT

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.77, no.6, pp.1262-1268, 2013-06-23 (Released:2013-06-23)

参考文献数

40

被引用文献数

23 8

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Short-chain-length medium-chain-length polyhydroxyalkanoate (SCL-MCL PHA) copolymers are promising as bio-plastics with properties ranging from thermoplastics to elastomers. In this study, the hybrid pathway for the biosynthesis of SCL-MCL PHA copolymers was established in recombinant Escherichia coli by co-expression of β-ketothiolase (PhaARe) and NADPH-dependent acetoacetyl-CoA reductase (PhaBRe) from Ralstonia eutropha together with PHA synthases from R. eutropha (PhaCRe), Aeromonas hydrophila (PhaCAh), and Pseudomonas putida (PhaC2Pp) and with (R)-specific enoyl-CoA hydratases from P. putida (PhaJ1Pp and PhaJ4Pp), and A. hydrophila (PhaJAh). When glycerol supplemented with dodecanoate was used as primary carbon source, E. coli harboring various combinations of PhaABCJ produced SCL-MCL PHA copolymers of various monomer compositions varying from C4 to C10. In addition, polymer property analysis suggested that the copolymers produced from this recombinant source have thermal properties (lower glass transition and melting temperatures) superior to polyhydroxybutyrate homopolymer.