著者

Kampei SHIMIZU Mika KUSHAMAE Tomohiro AOKI

出版者

The Japan Neurosurgical Society

雑誌

Neurologia medico-chirurgica (ISSN:04708105)

巻号頁・発行日

vol.59, no.7, pp.257-263, 2019 (Released:2019-07-15)

参考文献数

48

被引用文献数

6 13

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Considered with a poor outcome of subarachnoid hemorrhage due to rupture of intracranial aneurysms (IAs), treatment interventions to prevent rupture of the lesions are mandatory for social health. As treatment option is limited to surgical manipulations, like microsurgical clipping, endovascular coiling or deployment of flow diverter, and these surgical interventions have a potential risk of complications in nature, a proper selection of rupture-prone IAs among ones incidentally found is essential. Today, a rupture risk in each case is estimated by several factors like patient characteristics and morphological ones of each lesion. However, unfortunately, an IA without treatment sometimes unexpectedly ruptures resulting in a devastating outcome or an IA surgically treated is turned out to have a thick wall. To achieve more efficient treatment interventions, the development of a novel diagnostic modality is required. Here, mainly through the accumulation of experimental findings, the crucial contribution of macrophage-mediated chronic inflammatory responses to IA progression have been revealed, making macrophage being a promising target for a diagnosis. If we could non-invasively visualize accumulation of macrophages in lesions, this imaging technique ‘macrophage imaging’ may enable a qualitative evaluation of IAs to stratify rupture-prone ‘dangerous’ lesions among many stable ones. Thereby, a development of macrophage imaging makes an indication of surgical interventions being more accurate and also greatly facilitates a development of a novel medical therapy if used as a surrogate marker.

著者

鈴木 正昭 テップンポン マリーワン モラクン ポンレック 五十嵐 孝典

出版者

Japanese Society for Tropical Agriculture

雑誌

熱帯農業 (ISSN:00215260)

巻号頁・発行日

vol.26, no.2, pp.55-62, 1982-06-01 (Released:2010-03-19)

参考文献数

7

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タイ国の河川に繁茂するホテイアオイ (Eichhornia cyassipes, Solms) の実用的な堆肥化方法を検討した.すなわち, ホテイアオイ, 野草, 稲わら, 水牛糞などを主な材料とした各種の堆肥を製造し, その性状を経時的に調べて, 品質や熟度等を論じた.得られた結果は以下のように要約される.1.ホテイアオイだけを材料として堆肥を製造すると, 品温は42℃に達しただけであったが, 水牛糞や稲わらを混じて堆肥を製造すると容易に50℃以上の高温を得ることができた.2.ホテイアオイは窒素, リン酸, カリ等の養分に富むため, これを他の資材と混用することにより堆肥の品質が向上した.3.稲わらを堆肥化する場合にホテイアオイを混用すると, 水牛糞や石灰窒素を用いるのと同等の効果を発揮した.4.ホテイアオイは堆肥資材として良好であり, タイ国の農民に勧めうる.また, その適切な製造法について述べた.5. (付録) タイの中央平原と東北部で集めたホテイアオイを比較すると, 前者は後者よりも植物養分の含量が高い傾向を示した.茎葉部と根部について養分含量を比較すると, 茎葉部では窒素, リン酸, カリ, カルシウム, マグネシウム, 銅などが根部よりも高かったが, マンガンや亜鉛は根部の方が高かった.

著者

武田 昂樹 中川 朋 山田 晃正 小西 健 奥山 正樹 西嶌 準一

出版者

日本臨床外科学会

雑誌

日本臨床外科学会雑誌 (ISSN:13452843)

巻号頁・発行日

vol.76, no.6, pp.1314-1319, 2015 (Released:2015-12-28)

参考文献数

16

被引用文献数

1 3

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症例は40歳台,男性.201X年2月,1週間来の腹痛と嘔吐の増悪を主訴に当院を受診.来院時,腹部全体の圧痛・反跳痛を認めた.腹部X線画像で上行結腸周囲に多数の点状石灰化像を認めた.小腸niveau像を認めたが,free airは認めなかった.腹部造影CT検査では,右結腸静脈の石灰化に加え,上行結腸の腸管壁の造影不良と壁肥厚を認めたため,腸管壊死と診断して,緊急開腹手術を行った.開腹時所見では,上行結腸から横行結腸の中央部に腸管壊死を認め,結腸部分切除を行った.病理検査では,粘膜面の潰瘍形成,静脈血管壁の石灰化と上行結腸粘膜下層の広範な線維化領域を認め,特発性腸間膜静脈硬化症による虚血性大腸炎と診断した.術後経過は良好で,術後16病日に退院となった.本症例は10年以上の漢方薬(梔子柏皮湯®)の服用歴があり,病因との関連性が示唆された.現在,漢方薬内服を中止して経過観察中である.

著者

村田 祐樹 大見 卓司 倉持 梨恵子

出版者

一般社団法人 日本アスレティックトレーニング学会

雑誌

日本アスレティックトレーニング学会誌 (ISSN:24326623)

巻号頁・発行日

vol.5, no.2, pp.115-121, 2020-04-30 (Released:2020-06-05)

参考文献数

5

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青少年のスポーツにおいて,アスレティックトレーナー(AT)が関与することの重要性についての認識が高まっている.本邦でも一部の高校では,課外活動の質を向上させるために専任のATを雇用している.しかし,高校におけるATの業務内容とATが関与することの効果を示した報告はほとんどない.本研究の目的は,高校のATがスポーツ現場でどのような業務を行っているかをインタビューおよび自己開示によって明らかにすることであった.2名の私立学校に勤務するATが本研究に参加した.勤務形態は,1名が専任であり,もう1名が非常勤であった.対象者の主な業務は,スポーツ外傷・障害の評価,救急処置,リコンディショニング,スポーツ外傷・障害の予防であった.それらに加えて,スポーツ外傷・障害の予防と管理について,生徒,コーチ,教諭,養護教諭などに啓発することは,学校スポーツにおけるATの重要な役割であった.本研究は,学校現場でのATの介入事例を2例のみ示したものであり,高校の専任ATに関して更に多くの情報が必要である.したがって,高校においてATが関与する状況を明らかにするためには,体系的な調査を実施する必要があると考えられた.

著者

三島 徳三

出版者

Japan Society for Distributive Sciences

雑誌

流通 (ISSN:09149937)

巻号頁・発行日

vol.1992, no.5, pp.31-39, 1992 (Released:2010-08-05)

著者

Hiroyuki TAKAO Kenichiro SAKAI Hidetaka MITSUMURA Teppei KOMATSU Ichiro YUKI Kohei TAKESHITA Kenichi SAKUTA Toshihiro ISHIBASHI Teppei SAKANO Yuchih YEH Kostadin KARAGIOZOV Marc FISHER Yasuyuki IGUCHI Yuichi MURAYAMA

出版者

The Japan Neurosurgical Society

雑誌

Neurologia medico-chirurgica (ISSN:04708105)

巻号頁・発行日

vol.61, no.4, pp.260-267, 2021 (Released:2021-04-15)

参考文献数

18

被引用文献数

7

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Since smartphone applications are revolutionizing telemedicine, a new application specifically for stroke care (JOIN) was designed. Addition of the JOIN smartphone application to the stroke treatment workflow in our hospital was assessed. JOIN has key functions that may improve the care of stroke patients, including the ability to (1) exchange information such as patient data and medical images in real-time throughout the entire process of patient management; (2) track each step of the protocol from door to discharge; and (3) facilitate real-time interaction of all team members via text, audio, and a video chat system. Two periods, 2.7 years before the implementation of JOIN (Pre-JOIN) with 37 patients and 2.2 years after (Post-JOIN) with 54 patients, were compared, and the workflow for all 91 patients who had a cerebral infarction and were treated with tissue plasminogen activator (tPA) and/or thrombectomy between October 2012 and July 2017 was reviewed. There were noticeable reductions in overall patient management time, including times for door-to-imaging, starting tPA treatment, and endovascular intervention with JOIN. Staff members were unanimously satisfied with JOIN, due to the increased efficiency of information exchange and the ability for real-time discussions with different professionals when needed. No significant changes in patient outcomes (as assessed by modified Rankin Scale [mRS] scores) at 3 months and in the total cost for the treatment were observed. A smartphone-based application with the capability of sharing information instantaneously among healthcare professionals facilitated time-sensitive, acute care of ischemic stroke patients.

著者

稲垣 直樹

出版者

公益社団法人 日本薬理学会

雑誌

日本薬理学雑誌 (ISSN:00155691)

巻号頁・発行日

vol.131, no.1, pp.22-27, 2008 (Released:2008-01-11)

参考文献数

30

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近年,アレルギー疾患患者の数は増加の一途をたどっており,アレルギーを克服するためのより確実な戦略の構築が望まれている.自然免疫の機構についての解析が飛躍的に進み,微生物産物を認識する受容体および細胞内シグナル伝達経路が明らかになってきた.自然免疫の機構は獲得免疫の誘導にも役割を演じることから,アレルギー克服の手がかりが得られる可能性を有する.アレルギー疾患は背景にTh2細胞優位なTh1/Th2バランスを有するとされており,バランスを矯正することがアレルギー克服につながると考えられる.また,制御性T細胞の誘導あるいは移入が免疫応答の過剰発現であるアレルギーの制御に有用であると推定される.アレルギーの発症,進展,増悪には種々の遺伝因子が関与するが,遺伝因子の発現制御もアレルギー克服に役立つと思われる.一方,強力なアレルギー性炎症抑制効果を発揮するステロイドなど,既存の薬物の効果的な使用方法も検討されている.

著者

小野 伴忠

出版者

日本酪農科学会

雑誌

酪農科学・食品の研究 (ISSN:03850218)

巻号頁・発行日

vol.43, no.4, pp.A-65-A-71, 1994 (Released:2015-10-31)

参考文献数

54

著者

Ana Mafalda Abrantes Beatriz Nogueira-Garcia Mariana Alves Dúlio Teixeira Passos Dulce Brito Fausto J. Pinto Daniel Caldeira

出版者

The Japanese Circulation Society

雑誌

Circulation Reports (ISSN:24340790)

巻号頁・発行日

vol.3, no.8, pp.457-464, 2021-08-10 (Released:2021-08-10)

参考文献数

22

被引用文献数

5

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Background:Recent studies have revealed the benefits of using colchicine, a drug with anti-inflammatory properties, in coronary artery disease (CAD). This study systematically reviewed the benefits and risks of low-dose colchicine in patients with CAD.Methods and Results:We searched for randomized controlled trials (RCTs) in MEDLINE, the Cochrane Central Register of Controlled Trials (CENTRAL), and Web of Science databases (March 2020). Efficacy and safety outcomes were evaluated. Estimates are expressed as risk ratios (RRs) and 95% confidence intervals (95% CIs). Heterogeneity was assessed with I2test. Confidence in the pooled evidence was appraised using the GRADE framework. Colchicine reduced the rate of major adverse cardiovascular events (RR 0.65; 95% CI 0.49–0.86; 6 RCTs; I2=50%; 11,718 patients; GRADE, moderate confidence), acute coronary syndrome (RR 0.64; 95% CI 0.46–0.90; I2=47%; 7 RCTs; 11,955 patients; GRADE, very low confidence), stroke (RR 0.49; 95% CI 0.30–0.78; I2=0%; 6 RCTs; 11,896 patients; GRADE, moderate confidence), and cardiovascular interventions (RR 0.61; 95% CI 0.42–0.89; I2=40%; 4 RCTs; 11,284 patients; GRADE, high confidence). Colchicine did not increase the risk of adverse events, except for gastrointestinal events (RR 1.54; 95% CI 1.11–2.13; I2=72%; 9 RCTs; 12,374 patients; GRADE, very low confidence).Conclusions:Low-dose colchicine in patients with CAD is associated with beneficial effects on prognosis, although an increased risk of gastrointestinal events was confirmed.

著者

井若 和久 上月 康則 山中 亮一 田邊 晋 村上 仁士

出版者

Japan Society of Civil Engineers

雑誌

土木学会論文集B2(海岸工学) (ISSN:18842399)

巻号頁・発行日

vol.67, no.2, pp.I_1261-I_1265, 2011 (Released:2011-11-09)

参考文献数

16

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We examined values and applications of earthquake and tsunami stone monuments in Tokushima. The results of the study are as follows; (1) the purposes of stone utilization were to hand down records and lessons of earthquake and tsunami to posterity for its "presence", "conspicuousness" and "durability". (2) We recognized three cultural values of earthquake and tsunami stone monuments in Tokushima such as academic material, disaster prevention education material and disaster cultural asset. (3) We found that about 70% of earthquake and tsunami stone monuments in Tokushima have problems of their location and decipherment, and "teaching" function decreased remarkably. On the other hand, some stone monuments signs of their locations and/or explanations in modern Japanese were newly set up. (4) We collected the application cases of the earthquake and tsunami stone monuments in local municipals, areas and primary and junior high schools.

著者

中西 浩一 吉川 徳茂

出版者

一般社団法人 日本小児腎臓病学会

雑誌

日本小児腎臓病学会雑誌 (ISSN:09152245)

巻号頁・発行日

vol.25, no.2, pp.127-131, 2012-11-30 (Released:2012-12-22)

参考文献数

9

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繊毛は細胞表面から突出する小器官であり,気管などに存在する運動性の繊毛と,ほぼすべての細胞に存在する非運動性の一次繊毛が存在する。一次繊毛の役割は長らく不明であったが,近年,一次繊毛とその関連構造物の遺伝子変異により腎嚢胞,肝臓・胆管異常,内臓逆位,多指症,脳梁低形成,認知障害,網膜色素変性症,頭蓋・骨格異常,糖尿病など多岐にわたる異常を生じることが明らかになった。これら一群の繊毛機能不全疾病を称して繊毛病(ciliopathy)と呼ぶ。腎徴候を呈する繊毛病としては,常染色体優性および劣性多発性嚢胞腎,ネフロン癆,Joubert syndrome,Bardet-Biedl syndrome,Meckel-Gruber syndromeなどがある。繊毛病の多くは腎嚢胞を合併するため,腎嚢胞は繊毛病の診断上重要である。

著者

村上 正人

出版者

一般社団法人 日本心身医学会

雑誌

心身医学 (ISSN:03850307)

巻号頁・発行日

vol.61, no.5, pp.434-442, 2021 (Released:2021-07-01)

参考文献数

22

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リウマチ性疾患は自己免疫, アレルギーなどの免疫応答の異常による慢性炎症性疾患であり, 機能性リウマチ性疾患である線維筋痛症は慢性疼痛, 心身症のモデル的疾患である.いずれも遺伝的要因, 環境要因, 心身のストレス要因などが発症と経過に関係し, 病態理解と診断, 治療のためには心身医学的な視点を必要とする疾患である. これらの疾患は人種, 民族を超えて共通の病態を呈していながら, 疾患のとらえ方や対応はさまざまであり, 世界的な流れをみるには国際的な情報交換や情報発信が重要である. 筆者はリウマチ学, 心身医学の専門的立場からリウマチ性疾患, 特に線維筋痛症の臨床に携わってきたが, 米国留学, 国際学会への出席, ドイツ心身医学会や他国の研究者との相互交流などを通して多くの心身医学的知見を得た. 日本では厚生労働省研究班, 日本線維筋痛症学会や研究会, 患者会が設立され, それぞれに心身医学的視点が重要視されるなど, 国際的にもユニークな展開をみせているが, これらの成果の国外への情報発信は十分とはいえない. 今後はより多くの研究者の参画を得て日本の心身医学の国際化を図りたい.

著者

村上 正人 金 外淑

出版者

一般社団法人 日本内科学会

雑誌

日本内科学会雑誌 (ISSN:00215384)

巻号頁・発行日

vol.108, no.10, pp.2077-2087, 2019-10-10 (Released:2020-10-10)

参考文献数

11

被引用文献数

1

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線維筋痛症は,臨床検査や画像検査で異常所見が認められないため,自覚症状から診断せざるを得ないリウマチ性疾患の代表的な疾患であり,慢性疼痛のモデル的疾患でもある.中高年の女性に多く発症し,全身の筋肉や腱等の結合組織の痛みを中心に多彩な心身の愁訴を有するために,十分な鑑別診断が必要であるが,近年注目され始めた「機能性身体症候群」の概念が線維筋痛症の病態を理解するうえで有用である.リウマチ性疾患のなかでの線維筋痛症の位置付けや機能性身体症候群との関わり,診断と治療について論ずる.

著者

近藤 真前 清水 謙祐 五島 史行 北原 糺 今井 貴夫 橋本 誠 下郡 博明 池園 哲郎 中山 明峰

出版者

一般社団法人 日本めまい平衡医学会

雑誌

Equilibrium Research (ISSN:03855716)

巻号頁・発行日

vol.75, no.6, pp.489-497, 2016-12-31 (Released:2017-02-01)

参考文献数

29

被引用文献数

2 2

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Introduction: The vertigo symptom scale-short form (VSS-sf), which has three factors, the vestibular-balance symptom with long duration, the vestibular-balance symptom with short duration, and the autonomic symptom, was developed for measurement of the therapeutic effect in vestibular diseases. However, clinical use of the VSS-sf has not been reported in Japan, and there are very few analyses using scores of the factors or each item of the VSS-sf. The aims of this study is to report both clinical use of the VSS-sf in Japan and analyses using scores of the factors or each item of the VSS-sf. Methods: Participants included both adult inpatients and outpatients with either non-central dizziness/vertigo or vertebrobasilar insufficiency which occurred more than one month before, diagnosed by expert neuro-otologists. Participants completed three questionnaires: the VSS-sf, the dizziness handicap inventory, and the hospital anxiety and depression scale (HADS). We conducted a multiple regression analysis with the scores of the three factors of the VSS-sf, to evaluate how much influence there was from vestibular and autonomic symptoms on any handicap due to dizziness. We analyzed the scores of each item of the VSS-sf to examine profiles of the symptoms in major vestibular diseases. Results: The results of 159 participants were analyzed. Standard partial regression coefficients of anxiety, depression, and the vestibular-balance symptom with long duration were significant, however, those of the vestibular-balance symptom with short duration and the autonomic symptom were not. Most frequent autonomic symptoms were headache, chill/flashes, and palpitation in Ménière's disease, benign paroxysmal positional vertigo, vestibular neuronitis, and psychogenic dizziness. Conclusion: The VSS-sf can be conducted without major problems in Japan, and may be useful for patients with vestibular diseases, not only to measure therapeutic effect but also to analyze the influence of, or relation between the vestibular-balance symptom/autonomic symptoms and other clinical variables.

著者

佐伯 尚志 岩本 伸一

出版者

The Institute of Electrical Engineers of Japan

雑誌

電気学会論文誌B(電力・エネルギー部門誌) (ISSN:03854213)

巻号頁・発行日

vol.112, no.9, pp.765-772, 1992-09-20 (Released:2008-12-19)

参考文献数

11

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When analyzing dynamical stability in power systems, there exist various errors. First, there are analyzing errors which occur in linearlizing non-linear systems on a particular operating condition. Secondly, there are adjusting errors of the parameter values which are the time constants and the gains of the control systems. However, the conventional representative techniques, such as the eigen value method, the Routh-Hurwitz method and the Nyquist method, cannot deal with these kinds of errors. Using these representative methods, if the parameters vary, we have to recalculate the whole system stability. In order to solve this kind of problem, this paper proposes a use of the Kharitonov theorem which is one of the robust control theories.

著者

加登岡 大希 崎山 直夫 瀬能 宏

出版者

神奈川県立生命の星・地球博物館(旧神奈川県立博物館)

雑誌

神奈川自然誌資料 (ISSN:03889009)

巻号頁・発行日

vol.2019, no.40, pp.59-65, 2019 (Released:2019-09-01)

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Morphological changes accompanying the growth of the shortspine spurdog, Squalus mitsukurii (Squaliformes: Squalidae) were examined in ten specimens of 304.1 to 952.6 mm in total length (TL), collected from Sagami and Suruga bays, Japan. The caudal fin of specimens less than 330 mm TL has a broad white margin on the upper lobe, and the lower lobe is overall white. The upper lobe changes from having a rounded tip to a pointed tip at total lengths between about 450 to 500 mm, and the white posterior margin of the caudal fin also narrows in specimens of this size. The dermal denticles on the anterior part of the first dorsal fin in smaller specimen are arranged with a space between each one, bearing a tricuspid leaf-like portion and a stalk at the base. On the other hand, the dermal denticles change to a rhomboidal shape and become arranged in a muriform pattern without spaces between them in larger specimens. In comparison with Squalus sp. 2 specimens less than 324.1 mm TL, it is suggested that S. mitsukurii specimens of the same size are distinguished in having the second dorsal spine less covered with dermal denticles.

著者

宇治 康直 秋山 哲一

出版者

一般社団法人 日本建築学会

雑誌

日本建築学会技術報告集 (ISSN:13419463)

巻号頁・発行日

vol.27, no.66, pp.1021-1026, 2021-06-20 (Released:2021-06-20)

参考文献数

5

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This research investigates elements of uncertainty (EOU) remaining after demolition work starts, through observation of the design and construction processes of an exclusively-owned dwelling unit of a condominium with a design–bid–build method. Design revision was assumed for designers to deal with EOU as of the construction contract. There was a case where EOU found after the demolition of interior brought design revision and additional construction, which influenced the construction period and cost. It is thinkable that issues caused by EOU found during the construction work increase. A contract needs to be thought for such risks among stakeholders.

著者

有馬 雄祐

出版者

日本建築学会

雑誌

日本建築学会環境系論文集 (ISSN:13480685)

巻号頁・発行日

vol.86, no.785, pp.680-691, 2021-07-30 (Released:2021-07-30)

参考文献数

48

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Studies about well-being (WB) have been used to build an interdisciplinary research area centered on positive psychology and economics of happiness. WB research is characterized by active use of subjective data about one’s life, called subjective wellbeing (SWB), as indicators of the quality of an individual’s life or their society. SWB has various domains, including ones related to cognitive and emotional well-being, and each has different determinants. For example, life satisfaction, which is the cognitive aspect of SWB, is strongly correlated with income, while emotional well-being has a relatively strong correlation with health and social relationships. There are various theories about SWB’s composition, and the OECD has defined three basic domains of SWB: life evaluation (life satisfaction), emotion (affect, mood), and eudaimonia. Conventional research for assessing residential environments has used “housing satisfaction” as a subjective indicator of housing quality, which belongs to the “cognitive evaluation” domain. However, based on the findings of WB research, it can be inferred that there are diverse subjective domains related to housing quality. Therefore, in the current study, we attempted to construct home-related subjective well-being (HOME-SWB) based on the OECD’s SWB definition: “home satisfaction,” “positive emotions at home,” “negative emotions at home,” and “eudaimonia derived from home.” “Home satisfaction” is the cognitive aspect of HOME-SWB, which is similar to the conventional subjective indicator, housing satisfaction. “Positive emotions at home” includes the frequency of positive emotional experiences at home, such as feeling happy, cheerful, or joyful, while “negative emotions at home” includes the frequency of negative emotional experiences, such as feeling depressed, stressed out, or lonely at home. “Eudaimonia derived from home” indicate to what extent residents obtain experiences of eudaimonic well-being from their homes, such as self-esteem and the sense that life is worth living. The purpose of this study is to investigate the current status of HOME-SWB among 4,000 residents in the Tokyo area and the determinants of each domain of HOME-SWB using ordinary least squares (OLS) regression analysis. Our assessment shows that HOME-SWB is closely related to demographics; for example, the relationship between home satisfaction and age is a U-shaped curve, which is similar to the well-known relationship between life satisfaction and age. Therefore, we conducted OLS regression by controlling demographic variables, including gender, age, and household income, and the results show that each domain of HOME-SWB has unique relationships with them. For example, the size of a house strongly affects home satisfaction but not positive emotions or eudaimonic aspects. Having a nice view from windows or a high level of thermal insulation has a relatively strong effect on emotional HOME-SWB. Proactive ways of living in a home, such as being picky about furniture and the interior of one’s home or frequently redecorating rooms, enhance the eudaimonic aspects, such as self-esteem and optimism. When we use conventional subjective information to measure housing satisfaction as an indicator of housing quality, it is noted that the importance of the housing elements that strongly affect cognitive well-being, such as the size of a house, are overestimated, while the importance of elements that have an impact on the emotional and eudaimonic aspects of HOME-SWB are underestimated. There are various subjective domains related to housing quality; therefore, we can conclude that we must measure various domains of HOME-SWB when assessing home-related well-being based on residents’ subjective information.

著者

興津 舜也 金光 香保子 浅野 聡

出版者

一般社団法人 日本建築学会

雑誌

日本建築学会技術報告集 (ISSN:13419463)

巻号頁・発行日

vol.27, no.66, pp.937-942, 2021-06-20 (Released:2021-06-20)

参考文献数

14

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The report introduces the present condition and problem of descriptions about the treatment of disaster hazard areas in basic guideline for urban renaissance and guideline for city planning. The targets are 4 prefectures in Tokai region. As the result of analysis, it has become clear that treatments of disaster hazard areas were different from each municipality because of directionality about the exclusion of guideline for city planning and kinds of disasters. In the future, not excluded areas need to be considered excluding and if impossible, disaster prevention measures need to be set in location normalization plan.

著者

Takuma ARIIZUMI Shiro MURATA Sotaro FUJISAWA Masayoshi ISEZAKI Naoya MAEKAWA Tomohiro OKAGAWA Takumi SATO Eiji OISHI Akira TANENO Satoru KONNAI Kazuhiko OHASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.83, no.4, pp.558-565, 2021 (Released:2021-04-09)

参考文献数

24

被引用文献数

8

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Poultry red mites (PRMs, Dermanyssus gallinae) are harmful ectoparasites that affect farmed chickens and cause serious economic losses in the poultry industry worldwide. Acaricides are used for PRM control; however, some PRMs have developed acaricide-resistant properties, which have indicated the need for different approaches for PRM control. Therefore, it is necessary to elucidate the biological status of PRMs to develop alternative PRM control strategies. Quantitative polymerase chain reaction (qPCR) allows analysis of the biological status at the transcript level. However, reference genes are preferable for accurate comparison of expression level changes given the large variation in the quality of the PRM samples collected in each farm. This study aimed to identify candidate reference genes with stable expression levels in the different blood feeding states and life stages of PRMs. First, we selected candidates based on the following criteria: sufficient expression intensity and no significant expression difference between fed and starved states. We selected and characterized seven candidate reference genes. Among them, we evaluated the gene expression stability between the starved and fed states using RefFinder; moreover, we compared their expression levels in each life-stage and identified two reference genes, Elongation factor 1-alpha (ELF1A)-like and apolipophorins-like. Finally, we evaluated the utility of the candidates as reference genes, and the use of ELF1A-like and apolipophorins-like successfully normalized ATP synthase subunit g -like gene expression. Thus, ELF1A-like and apolipophorins-like could be suitable reference genes in PRMs.